CN109507178A - A kind of environment-friendly microbial indole test instrument - Google Patents
A kind of environment-friendly microbial indole test instrument Download PDFInfo
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- CN109507178A CN109507178A CN201811586049.4A CN201811586049A CN109507178A CN 109507178 A CN109507178 A CN 109507178A CN 201811586049 A CN201811586049 A CN 201811586049A CN 109507178 A CN109507178 A CN 109507178A
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- ether
- glass bucket
- fermentation
- boiling tube
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention discloses a kind of environment-friendly microbial indole test instrument, including the Boiling tube that ferments, matching is fitted with test tube plug in the fermentation Boiling tube, and the top of fermentation Boiling tube is extended at the top of test tube plug, the fluid nutrient medium of identification bacterium is loaded in fermentation Boiling tube, sealing sliding sleeve is equipped with ether and collects horn mouth in the fermentation Boiling tube, ether collects bell-mouthed top and is fixedly installed with top as the glass bucket of opening setting, and the first circular hole is offered in the bottom interior wall of glass bucket, glass bucket collects horn mouth with ether by the first circular hole and is connected, sealing sliding sleeve is equipped with bottom cover in the glass bucket.The present invention has rational design, it is easy to operate, convenient for indole reagent is instilled in glass bucket, and then it is less by human factor, and then the phenomenon that can more accurately observing experimental result, reducing false negative, since ether layer and fermentation liquid efficiently separate, and then pollution of the ether to environment can be reduced, meet the use demand of personnel.
Description
Technical field
The present invention relates to microorganism indole test instrument technical field more particularly to a kind of environment-friendly microbial indole tests
Instrument.
Background technique
It is one of the experiment that must be done that in microbial physiology biochemical test, indoles, which generates experiment, when originally carrying out indole test
Indoles that tryptophan generates is extracted because ether is added and is enriched to ether layer, could be added later to dimethylamino benzene first
Aldehyde effect, generates red rosindol, and since the indoles amount of generation is limited, what is be not enriched with or be enriched with is bad, to observe
Good experimental result is all very difficult, in this way after the amount of dropwise addition ether, dropwise addition multiple oscillation, and the standing 1-3 after oscillation divides
Clock is all the technology that must be held very well, with greater need for very small when Paradimethylaminobenzaldehyde especially is added dropwise after ether layering
The indoles that careful slowly the adding just along test tube wall of the heart can guarantee extracting to ether layer will not re-mix in fermentation liquid, also
It can guarantee that indoles and Paradimethylaminobenzaldehyde effect produce red compound rosindol, never shake test tube at this time,
Ether layer is destroyed, otherwise red positive findings are also difficult to occur.
Existing biology indole test instrument is not easy to instill indole reagent in test tube, be easy there are still some shortcomings
By human factor cause false negative as a result, and be not easy to separate ether, in turn result in the pollution of environment, be not able to satisfy
The use demand of personnel, therefore we have proposed a kind of environment-friendly microbial indole test instrument for solving the above problems.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of environment-friendly microbial indole test instrument.
A kind of environment-friendly microbial indole test instrument proposed by the present invention, including fermentation Boiling tube, the fermentation Boiling tube
Interior matching is fitted with test tube plug, and the top of fermentation Boiling tube is extended at the top of test tube plug, is loaded with mirror in the Boiling tube that ferments
Determine the fluid nutrient medium of bacterium, sealing sliding sleeve is equipped with ether and collects horn mouth in the fermentation Boiling tube, and ether collects horn mouth
Top be fixedly installed with top as the glass bucket of opening setting, and the first circular hole is offered in the bottom interior wall of glass bucket, glass bucket is logical
It crosses the first circular hole to be connected with ether collection horn mouth, sealing sliding sleeve is equipped with bottom cover in the glass bucket, and the top of bottom cover is installed
There is switch lever, switch lever is matched with bottom cover, and the top of switch lever extends to the top open part of glass bucket, institute
It states sliding sleeve in fermentation Boiling tube and is equipped with support hard plug, and the fixation of hard plug is supported to be set on glass bucket, the top of the glass bucket
Portion side is rotatably equipped with upper cover, and the side of upper cover is fixedly installed with block, and a lateral roof of the glass bucket offers bayonet, and
Bayonet is matched with block.
Preferably, the ether collects fixing sleeve on horn mouth and is equipped with the first sealing ring, and the outside of the first sealing ring with
The side walls connection of fermentation Boiling tube.
Preferably, fixing sleeve is equipped with the second sealing ring, and the side wall in the outside of the second sealing ring and glass bucket on the bottom cover
It is slidably connected.
Preferably, first through hole, first through hole and the fixed suit of glass bucket are offered at the top of the support hard plug.
Preferably, the outside of the support hard plug is connect with the side walls of fermentation Boiling tube.
Preferably, the top side of the fermentation Boiling tube is fixedly installed with bent shape connection sheet, and the top of connection sheet
Portion and the other side of upper cover are fixedly mounted.
Preferably, the side wall movable contact in the outside of the test tube plug and fermentation Boiling tube.
Compared with prior art, the beneficial effects of the present invention are:
By fermentation Boiling tube, test tube plug, ether collect horn mouth, glass bucket, bottom cover, the second sealing ring, support hard plug,
First through hole, switch lever, upper cover, bayonet, block, connection sheet are matched with the first sealing ring, by strain to be identified to liquid
Heat insulating culture is carried out in body culture medium, then removes test tube plug, and ether is and then collected into horn mouth and the Boiling tube that ferments
Bayonet alignment presses down on upper cover, and upper cover drives glass bucket down position mobile, and glass bucket drives ether to collect horn mouth down position
It is mobile, so that ether collects horn mouth slowly at the liquid level of nearly fluid nutrient medium, upper cover is and then opened, switch manipulation is passed through
Bar opens bottom cover, is added dropwise few drops of ether by glass bucket channel, seals upper cover oscillation and fermentation Boiling tube, leads to after ether enters glass bucket
Switch lever closure bottom cover is crossed, ether layer is extracted, indole reagent can directly be added dropwise in glass bucket, and then can be more accurate
The yin and yang attribute for judging indole test collects horn mouth, glass bucket, upper cover, bayonet, card by fermentation Boiling tube, test tube plug, ether
Gear, connection sheet and fluid nutrient medium match, and go out ether layer by the extracted by ether in glass bucket, press down on upper cover, prevent second
Ether volatilization, since ether layer and fermentation liquid efficiently separate, and then can reduce pollution of the ether to environment, be conducive to making for personnel
With.
The present invention has rational design, easy to operate, convenient for indole reagent is instilled in glass bucket, so that it is less by human factor,
And then the phenomenon that can more accurately observing experimental result, reducing false negative, since ether layer and fermentation liquid efficiently separate, into
And pollution of the ether to environment can be reduced, meet the use demand of personnel.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of environment-friendly microbial indole test instrument proposed by the present invention;
Fig. 2 is a kind of schematic cross-sectional view of environment-friendly microbial indole test instrument proposed by the present invention;
Fig. 3 is the schematic cross-sectional view of part A in Fig. 2;
Fig. 4 is the schematic cross-sectional view of part B in Fig. 2;
Fig. 5 is the schematic cross-sectional view of C portion in Fig. 3.
In figure: 1 fermentation Boiling tube, 2 test tube plugs, 3 ether collect horn mouth, 4 glass buckets, 5 bottom covers, 6 second sealing rings, 7
Support hard plug, 8 first through hole, 9 switch levers, 10 upper covers, 11 bayonets, 12 blocks, 13 connection sheets, 14 fluid nutrient mediums, 15
First sealing ring.
Specific embodiment
Combined with specific embodiments below the present invention is made further to explain.
Embodiment
With reference to Fig. 1-5, the present embodiment proposes a kind of environment-friendly microbial indole test instrument, including fermentation Boiling tube 1, hair
Matching is fitted with test tube plug 2 in ferment Boiling tube 1, and the top of test tube plug 2 extends to the top of fermentation Boiling tube 1, big examination of fermenting
The fluid nutrient medium 14 of identification bacterium is loaded in pipe 1, sealing sliding sleeve is equipped with ether and collects horn mouth 3, second in fermentation Boiling tube 1
The top that ether collects horn mouth 3 is fixedly installed with top as the glass bucket 4 of opening setting, and offers in the bottom interior wall of glass bucket 4
First circular hole, glass bucket 4 collect horn mouth 3 with ether by the first circular hole and are connected, and sealing sliding sleeve is equipped with bottom cover 5 in glass bucket 4,
The top of bottom cover 5 is equipped with switch lever 9, and switch lever 9 is matched with bottom cover 5, and the top of switch lever 9 extends to
The top open part of glass bucket 4, ferment Boiling tube 1 in sliding sleeve be equipped with support hard plug 7, and support hard plug 7 fixation be set in
On glass bucket 4, the top side of glass bucket 4 is rotatably equipped with upper cover 10, and the side of upper cover 10 is fixedly installed with block 12, glass bucket 4
One lateral roof offers bayonet 11, and bayonet 11 is matched with block 12, is collected by fermentation Boiling tube 1, test tube plug 2, ether
Horn mouth 3, glass bucket 4, bottom cover 5, the second sealing ring 6, support hard plug 7, first through hole 8, switch lever 9, upper cover 10, bayonet
11, block 12, connection sheet 13 are matched with the first sealing ring 15, will carry out heat preservation training in strain to be identified to fluid nutrient medium 14
It supports, then removes test tube plug 2, ether is and then collected into horn mouth 3 and is aligned with the bayonet for the Boiling tube 1 that ferments, is pressed down on
Upper cover 10, upper cover 10 drive 4 down position of glass bucket mobile, and glass bucket 4 drives ether to collect the movement of 3 down position of horn mouth, so that
Ether collects horn mouth 3 slowly at the liquid level of nearly fluid nutrient medium 4, and then opens upper cover 10, is beaten by switch lever 9
Bottom cover 5 is opened, is added dropwise few drops of ether by 4 channel of glass bucket, 10 oscillation and fermentation Boiling tube 1 of upper cover is sealed, after ether enters glass bucket 4
It is closed bottom cover 5 by switch lever 9, ether layer is extracted, indole reagent can directly be added dropwise in glass bucket 4, and then can be more
The yin and yang attribute for accurately judging indole test collects horn mouth 3, glass bucket 4, upper cover by fermentation Boiling tube 1, test tube plug 2, ether
10, bayonet 11, block 12, connection sheet 13 and fluid nutrient medium 14 match, and go out ether layer by the extracted by ether in glass bucket 4,
Upper cover 10 is pressed down on, prevents ether from volatilizing, since ether layer and fermentation liquid efficiently separate, and then ether can be reduced to environment
Pollution, be conducive to the use of personnel, the present invention has rational design, easy to operate, convenient for indole reagent is instilled in glass bucket 4, into
And it is less by human factor, the phenomenon that and then can more accurately observing experimental result, reduce false negative, due to ether layer with
Fermentation liquid efficiently separates, and then can reduce pollution of the ether to environment, meets the use demand of personnel.
In the present embodiment, ether collects fixing sleeve on horn mouth 3 and is equipped with the first sealing ring 15, and the first sealing ring 15 is outer
Side is connect with the side walls of fermentation Boiling tube 1, and fixing sleeve is equipped with the second sealing ring 6 on bottom cover 5, and the second sealing ring 6 is outer
Side is connect with the side walls of glass bucket 4, and the top of support hard plug 7 offers first through hole 8, and first through hole 8 and glass bucket 4 are fixed
Suit supports the outside of hard plug 7 and the side walls of fermentation Boiling tube 1 to connect, the fixed peace of the top side of fermentation Boiling tube 1
Equipped with bent shape connection sheet 13, and the top of connection sheet 13 and the other side of upper cover 10 are fixedly mounted, the outside of test tube plug 2 with
The side wall movable contact of fermentation Boiling tube 1 passes through fermentation Boiling tube 1, test tube plug 2, ether and collects horn mouth 3, glass bucket 4, bottom cover
5, the second sealing ring 6, support hard plug 7, first through hole 8, switch lever 9, upper cover 10, bayonet 11, block 12, connection sheet 13
It is matched with the first sealing ring 15, heat insulating culture will be carried out in strain to be identified to fluid nutrient medium 14, then take test tube plug 2
Under, ether is and then collected into horn mouth 3 and is aligned with the bayonet for the Boiling tube 1 that ferments, upper cover 10 is pressed down on, upper cover 10 drives glass
4 down positions of bucket are mobile, and it is mobile that glass bucket 4 drives ether to collect 3 down position of horn mouth, so that ether collects horn mouth 3 slowly
To the liquid level of nearly fluid nutrient medium 4, upper cover 10 is and then opened, bottom cover 5 is opened by switch lever 9, it is logical by glass bucket 4
Road is added dropwise few drops of ether, seals 10 oscillation and fermentation Boiling tube 1 of upper cover, is closed after ether enters glass bucket 4 by switch lever 9
Bottom cover 5 extracts ether layer, indole reagent can directly be added dropwise in glass bucket 4, and then can more accurately judge indole test
Yin and yang attribute is collected horn mouth 3 by fermentation Boiling tube 1, test tube plug 2, ether, glass bucket 4, upper cover 10, bayonet 11, block 12, is connected
Contact pin 13 and fluid nutrient medium 14 match, and go out ether layer by the extracted by ether in glass bucket 4, press down on upper cover 10, prevent
Ether volatilization, since ether layer and fermentation liquid efficiently separate, and then can reduce pollution of the ether to environment, be conducive to personnel's
It uses, the present invention has rational design, and it is easy to operate, convenient for indole reagent is instilled in glass bucket 4, so that it is less by human factor, into
And the phenomenon that can more accurately observing experimental result, reducing false negative, since ether layer and fermentation liquid efficiently separate, in turn
Pollution of the ether to environment can be reduced, the use demand of personnel is met.
In the present embodiment, in use, routinely sterile working is inoculated with strain to be identified to preparatory sterilizing and cooling hair
Heat insulating culture is carried out in fluid nutrient medium 14 in ferment Boiling tube 1, is then removed the test tube plug 2 to ferment in Boiling tube 1, immediately
By ether collect horn mouth 3 with ferment Boiling tube 1 bayonet be aligned, press down on upper cover 10, upper cover 10 drive glass bucket 4 sending out
Down position is mobile in ferment Boiling tube 1, and glass bucket 4 drives the slide over side walls for supporting hard plug 7 in fermentation Boiling tube 1, while glass
Bucket 4 drives ether to collect, and the down position in fermentation Boiling tube 1 of horn mouth 3 is mobile, and ether collects horn mouth 3 and drives the first sealing
Circle 15 fermentation Boiling tube 1 slide over side walls so that ether collects horn mouth 3 slowly at the liquid level of nearly fluid nutrient medium 4,
And then upper cover 10 is opened, upper cover 10 drives block 12 to separate with bayonet 11, switch lever 9 is and then manipulated, so that switch
Control stick 9 opens bottom cover 5, is added dropwise few drops of ether by 4 channel of glass bucket, seals 10 oscillation and fermentation Boiling tube 1 of upper cover, and stand
1-3 minutes, after ether enters glass bucket 4, bottom cover 5 is closed by switch lever 9, indole reagent is directly added dropwise in glass bucket 4,
So that extract ether layer, since ether layer and fermentation liquid have efficiently separated, and then the influence that need not consider oscillation etc., downwards
Upper cover 10 is pressed, upper cover 10 drives block 12 to clamp with 11 phase of bayonet, prevents ether volatilization pollution environment, has red appearance, i.e.,
For the indole test positive, redfree is occurred being that indole test is negative, is concentrated in the ether in glass bucket, do not had to again due to product
Fall into a long wait, and sensitivity increases substantially, and then can more accurately observe experimental result, meets the use of personnel
Demand.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (7)
1. a kind of environment-friendly microbial indole test instrument, including fermentation Boiling tube (1), the interior matching of the fermentation Boiling tube (1) is inserted
The top of fermentation Boiling tube (1) is extended to equipped with test tube plug (2), and at the top of test tube plug (2), is contained in fermentation Boiling tube (1)
There is the fluid nutrient medium (14) of identification bacterium, which is characterized in that sealing sliding sleeve is collected equipped with ether in the fermentation Boiling tube (1)
Horn mouth (3), ether, which is collected, is fixedly installed with top as the glass bucket (4) of opening setting at the top of horn mouth (3), and glass bucket (4)
Bottom interior wall on offer the first circular hole, glass bucket (4) is connected by the first circular hole with ether collection horn mouth (3), described
Sealing sliding sleeve is equipped with bottom cover (5) in glass bucket (4), is equipped with switch lever (9) at the top of bottom cover (5), switch lever (9)
It is matched with bottom cover (5), the top of switch lever (9) extends to the top open part of glass bucket (4), the fermentation Boiling tube
(1) interior sliding sleeve is equipped with support hard plug (7), and hard plug (7) fixation is supported to be set on glass bucket (4), the glass bucket (4)
Top side is rotatably equipped with upper cover (10), and the side of upper cover (10) is fixedly installed with block (12), the side of the glass bucket (4)
Top offers bayonet (11), and bayonet (11) is matched with block (12).
2. a kind of environment-friendly microbial indole test instrument according to claim 1, which is characterized in that the ether collects loudspeaker
Fixing sleeve is equipped with the first sealing ring (15) on mouth (3), and the side wall in the outside of the first sealing ring (15) and fermentation Boiling tube (1)
It is slidably connected.
3. a kind of environment-friendly microbial indole test instrument according to claim 1, which is characterized in that on the bottom cover (5)
Fixing sleeve is equipped with the second sealing ring (6), and the outside of the second sealing ring (6) is connect with the side walls of glass bucket (4).
4. a kind of environment-friendly microbial indole test instrument according to claim 1, which is characterized in that the support hard plug
(7) it is offered at the top of first through hole (8), first through hole (8) and glass bucket (4) fixed suit.
5. a kind of environment-friendly microbial indole test instrument according to claim 1, which is characterized in that the support hard plug
(7) outside is connect with the side walls of fermentation Boiling tube (1).
6. a kind of environment-friendly microbial indole test instrument according to claim 1, which is characterized in that the fermentation Boiling tube
(1) top side is fixedly installed with bent shape connection sheet (13), and the top of connection sheet (13) and upper cover (10) is another
Side is fixedly mounted.
7. a kind of environment-friendly microbial indole test instrument according to claim 1, which is characterized in that the test tube plug (2)
Outside and fermentation Boiling tube (1) side wall movable contact.
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CN201811586049.4A CN109507178B (en) | 2018-12-25 | 2018-12-25 | Environment-friendly microbial indole tester |
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CN201811586049.4A CN109507178B (en) | 2018-12-25 | 2018-12-25 | Environment-friendly microbial indole tester |
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CN109507178B CN109507178B (en) | 2021-03-19 |
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WO2014051037A1 (en) * | 2012-09-28 | 2014-04-03 | 積水メディカル株式会社 | Tube body, blood collection tube, and method for manufacturing tube body |
CN106520539A (en) * | 2016-12-05 | 2017-03-22 | 菏泽学院 | Multi-function instrument used for microbe acid and gas producing experiment and sulfur-containing amino acid detection experiment |
CN207227429U (en) * | 2017-09-14 | 2018-04-13 | 成都康辉生物科技有限公司 | A kind of fungi preservation device easy to operation |
CN108392224A (en) * | 2018-02-08 | 2018-08-14 | 江南大学附属医院 | A kind of mobile type endocrine takes checking device integrated box |
CN108721961A (en) * | 2018-06-12 | 2018-11-02 | 广州市雷德生物科技有限公司 | A kind of filter device of blood |
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2018
- 2018-12-25 CN CN201811586049.4A patent/CN109507178B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014051037A1 (en) * | 2012-09-28 | 2014-04-03 | 積水メディカル株式会社 | Tube body, blood collection tube, and method for manufacturing tube body |
CN106520539A (en) * | 2016-12-05 | 2017-03-22 | 菏泽学院 | Multi-function instrument used for microbe acid and gas producing experiment and sulfur-containing amino acid detection experiment |
CN207227429U (en) * | 2017-09-14 | 2018-04-13 | 成都康辉生物科技有限公司 | A kind of fungi preservation device easy to operation |
CN108392224A (en) * | 2018-02-08 | 2018-08-14 | 江南大学附属医院 | A kind of mobile type endocrine takes checking device integrated box |
CN108721961A (en) * | 2018-06-12 | 2018-11-02 | 广州市雷德生物科技有限公司 | A kind of filter device of blood |
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