CN201272809Y - Microorganism resistance test apparatus for plasma sterilizing validation - Google Patents
Microorganism resistance test apparatus for plasma sterilizing validation Download PDFInfo
- Publication number
- CN201272809Y CN201272809Y CNU2008201642621U CN200820164262U CN201272809Y CN 201272809 Y CN201272809 Y CN 201272809Y CN U2008201642621 U CNU2008201642621 U CN U2008201642621U CN 200820164262 U CN200820164262 U CN 200820164262U CN 201272809 Y CN201272809 Y CN 201272809Y
- Authority
- CN
- China
- Prior art keywords
- tank
- resistance test
- plasma sterilization
- test apparatus
- verification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The utility model relates to a microbiological resistance test device for plasma sterilization tests. The technical problem to be solved includes providing a microbiological resistance test device and a method thereof for plasma sterilization tests, aiming to research on sterilization resistance of standard microbiological strains used after the hydrogen oxide plasma sterilization test through the device and the method, thereby comparing and selecting strains with strongest resistance as the standard test strains. The technical scheme for solving the problem includes that a resistance detecting device and a temperature sensor II are mounted in a test chamber, a neutralizing agent filler and a sterilizing medium filler are connected to the external of the test chamber, the neutralizing agent filler is communicated with the resistance detecting device through a sealing joint on the test chamber wall by a connecting duct I, and the sterilizing medium filler is connected to an atomizing nozzle arranged on the upper side of the wall of a vacuum tank through a micro-electromagnetic valve by a connecting duct II. The utility model can be applied to hydrogen peroxide plasma sterilization tests.
Description
Technical field
The utility model relates to the drag experimental installation, especially a kind of microorganism resistance test apparatus that is used for verification of plasma sterilization.Not only be applicable to the hydrogen peroxide plasma sterile test, also be applicable to the sterilization drag test of the microbial strains of multiple medical cryogenic gas sterilising method checking.
Background technology
The hydrogen peroxide plasma temperature sterilization is present up-to-date in the world a kind of medical temperature sterilization technology, compare with other conventional medical sterilization technology, have characteristics such as low temperature is quick, energy-conserving and environment-protective, economic security, can be used for clinical departments such as hospital operating room, Supply House, sight glass chamber various medical devices (comprising metallic weapon and nonmetal apparatus) is carried out quick temperature sterilization processing.
As medical sterilizing device, need carry out verification experimental verification to the sterilization usefulness of this equipment and technological method, this just need carry out proof test by the selected the strongest standard bacterial classification of a kind of drag of correlation test.And selected which kind of bacterial classification, then need to measure multiple microbial strains the drag index D value of this sterilising method (is killed the needed time of 90% microorganism, the D value is big more, illustrate that this bacterial classification is strong more to the drag of this sterilising method), thereby the usefulness proof test is used to sterilize relatively and select the strongest bacterial classification of drag as the standard test bacterial classification.Because hydrogen peroxide plasma temperature sterilization technology is a new technology, at present, still there is not a kind of easy, reliable testing apparatus to carry out the drag test of above-mentioned checking bacterial classification.
The utility model content
The technical problems to be solved in the utility model is: a kind of microorganism resistance test apparatus that is used for verification of plasma sterilization is provided, be intended to by this device carrying out the research of the relevant drag of sterilizing through the used standard microorganism bacterial classification in hydrogen peroxide plasma sterilization test back, thereby relatively and select the strongest bacterial classification of drag as the standard test bacterial classification.
The technical scheme that the utility model adopted is: the microorganism resistance test apparatus that is used for verification of plasma sterilization, have vacuum tank and hermatic door, thereby form the experimental tank chamber, inwall in the experimental tank chamber is equipped with discharge electrode and is connected with coupling tuner and plasma exciatiaon power supply with concentric cable by the sealing feed-in part on the tank body, and the plasma exciatiaon power supply is connected with electric apparatus control apparatus again; The sidewall of described vacuum tank is provided with and vacuumizes interface, this interface is connected with vacuum pump by the high vacuum flapper valve, the other end connecting circuit control device of vacuum pump, also is furnished with the inflation interface on the described tank skin, this interface is connected the other end connecting circuit control device of high vacuum flapper valve by the high vacuum flapper valve with air filter; Also equipped electrothermic constant temperature equipment and vacuum detecting transmitter on the outer side wall of described vacuum tank, it is characterized in that described experimental tank is indoor is equipped with the drag proofing unit and is furnished with temperature sensor II, be connected to neutralizing agent charging point and sterile medium charging point in the outside of experimental tank chamber, described neutralizing agent liquid-adding device is communicated with the drag proofing unit by the sealed interface of linking conduit I on the experimental tank locular wall, and described sterile medium filling apparatus is connected to the dispersive nozzle that is located at vacuum tank tank skin top by linking conduit II through miniature electromagnetic valve.
Described drag proofing unit has the mechanical drive that is fixed on middle part, experimental tank chamber, this device is by direct-current machine, wheel box, turn-screw, driving nut, motion bar, axle sleeve and overcoat are formed, outer putting in its vertical fixing is fixed with lower fixed frame and at the one end movable block down is housed, be fixed with upper holder on the motion bar of mechanical drive and movable block and micro valve be housed at the one end, motion bar outside on upper holder is with spring and on its top gland is housed, and is provided with fixed briquetting and active spill spin block between this gland and micro valve.
Described electrothermic constant temperature equipment is made up of the hot plate and the thermal insulation layer that are located at the vacuum tank outside, and is furnished with temperature sensor and temperature controller.
Described vacuum tank is made by stainless steel, titanium alloy or aluminium alloy, is shaped as drum shape or rectangle, vertical or horizontal placement, and volume is 30～150L.
Described discharge electrode is made by stainless steel, titanium alloy or aluminium alloy porous plate, with the tank skin gap be 5～50mm, and insulate with tank skin.
Described neutralizing agent charging point comprises 1～5 syringe and liquid feeding carriage, has the substratum that contains neutralizing agent of 5～10ml in each syringe.
Described sterile medium charging point comprises syringe and liquid feeding carriage.
Near described neutralizing agent charging point and the sterile medium charging point temperature sensor I is housed.
The beneficial effects of the utility model are: the utility model provides a kind of microorganism resistance test apparatus and method for the technical identification of hydrogen peroxide plasma temperature sterilization, can optimize the strongest bacterial classification of drag easy, reliably by this testing apparatus, thereby carry out the experimental study of relevant sterilising effect checking as the standard test bacterial classification.
Description of drawings
Fig. 1 is that structure of the present utility model is always schemed.
Fig. 2 is the structure iron (rubber seal plug is in sealed state) of a kind of state of drag proofing unit in the utility model.
Fig. 3 is the structure iron (rubber seal plug is in opened condition) of the another kind of state of drag proofing unit in the utility model.
Embodiment
As shown in Figure 1-Figure 3, present embodiment has vacuum tank 1 and hermatic door 2, thereby form experimental tank chamber 3, inwall in the experimental tank chamber is equipped with discharge electrode 4 and is connected with coupling tuner 9 and plasma exciatiaon power supply 10 with concentric cable by the sealing feed-in part on the tank body, and the plasma exciatiaon power supply is connected with electric apparatus control apparatus 12 again.Described discharge electrode 4 is made by metallic porous sheets such as stainless steel, titanium alloy or aluminium alloys, with the tank skin gap be 5～50mm, and with tank skin insulation, so-called plasma exciatiaon discharge is exactly to pass through discharge electrode 4, under the control of program in the glow discharge of the indoor formation plasma body of test tank.Output power of power supply is adjustable in 50～500W scope, and discharge time, control accuracy was ± 0.1 second.
The sidewall of described vacuum tank 1 is provided with and vacuumizes interface 6, and this interface is connected the other end connecting circuit control device 12 of vacuum pump by high vacuum flapper valve 8 with vacuum pump 7; Also be furnished with inflation interface 24 on the described tank skin, this interface is connected the other end connecting circuit control device 12 of high vacuum flapper valve 8 by high vacuum flapper valve 8 with air filter 11.Vacuum control unit is opened vacuum pump and each valve by time variable control, can make fast to vacuumize in the jar to reach specified vacuum value (30～300Pa).Being provided with of inflation interface 24 can make testing laboratory recover concora crush in a short period of time, so that opening encapsulation door and take out withstand voltage test tube 21 fast.
Also equipped electrothermic constant temperature equipment 5 and vacuum detecting transmitter 13 on the outer side wall of described vacuum tank 1, this electrothermic constant temperature equipment 5 is made up of the hot plate and the thermal insulation layer that are located at vacuum tank 1 outside, and be furnished with temperature sensor and temperature controller, the warranty test chamber wall temperature is controlled in ℃ scope of room temperature～80.The temperature display precision is 0.1 ℃.
Drag proofing unit 20 is installed in the described experimental tank chamber 3 and is furnished with temperature sensor II14-2.Described drag proofing unit 20 has the mechanical drive 23 that is fixed on 3 middle parts, experimental tank chamber, this device is by direct-current machine 23-1, wheel box 23-2, turn-screw 23-3, driving nut 23-4, motion bar 23-5, axle sleeve 23-6 and overcoat 23-7 form, on the overcoat 23-7 of its vertical fixing, be fixed with lower fixed frame 20-9 and movable block 20-1 down be housed at the one end, on the motion bar 23-5 of mechanical drive 23, be fixed with upper holder 20-8 and movable block 20-2 and micro valve 20-3 be housed at the one end, motion bar 23-5 outside on upper holder 20-8 is with spring 20-7 and on its top gland 20-6 is housed, and is provided with fixed briquetting 20-4 and active spill spin block 20-5 between this gland and micro valve 20-3.
In order accurately to finish sterilization process, eliminate the subsequent affect of sterile medium to microorganism, need be set based on the test requirement at regular time and quantity at the withstand voltage neutralizing agent of in vitro annotating, therefore, this example is connected to neutralizing agent charging point 16 in the outside of experimental tank chamber 3, and described neutralizing agent liquid-adding device is communicated with the drag proofing unit by the sealed interface of linking conduit I15-1 on the experimental tank locular wall.Described neutralizing agent charging point 16 comprises 5 syringes and liquid feeding carriage, has the substratum that contains neutralizing agent of 5～10ml in each syringe.When the drag test stopped, program was opened the micro valve 20-3 on the withstand voltage test tube sealing plug automatically, neutralizing agent is joined in vitro, and submergence bacterium sheet was to reach the purpose that stops bactericidal reaction.
Sterile medium of the present utility model is a hydrogen peroxide liquid, becomes gaseous state again and evenly spreads need join the indoor and atomizing of experimental tank at regular time and quantity in sterilization process after, realizes the purpose of killing microorganisms.Therefore, be connected to sterile medium charging point 17 in the outside of experimental tank chamber 3, this sterile medium charging point 17 is connected to the dispersive nozzle 19 that is located at vacuum tank tank skin top by linking conduit II15-2 through miniature electromagnetic valve 18.Described sterile medium charging point 17 comprises syringe and liquid feeding carriage.The time requirement that is set based on the test is controlled automatic liquid feeding by miniature electromagnetic valve, and time control can be accurate to 0.1 second, and this sterile medium charging point can be controlled at the hydrogen peroxide injection rate in 0.1～5ml scope accurately adjustable.
Described vacuum tank 1 is made by metallic substance such as stainless steel, titanium alloy or aluminium alloys, is shaped as drum shape or rectangle, vertical or horizontal placement, and volume is 30～150L; Be furnished with metal or nonmetal vacuum-sealing door 2, it is convenient to open;
Near described neutralizing agent charging point 16 and the sterile medium charging point 17 temperature sensor I14-1 is housed.
Electric apparatus control apparatus 12 in this example constitutes control circuit board by micro-chip unicircuit and color liquid crystal touch display screen, constitute automatic running gear by functional devices such as rly. and alternating current contactors again, by the program software in the singlechip chip, realize the automatic operating of complete assembly system.Described program software is the basic demand according to microbiology sterilising plant drag test, in conjunction with the utility model apparatus features and function, and the specific routine software of being worked out, and realize the automatic operation of complete assembly by single-chip computer control system.
Present embodiment is a kind of microorganism resistance test method that is used for verification of plasma sterilization, has comprised following steps:
1) the substratum 10mL that will contain neutralizing agent is one by one in 5 (5 of this examples are one group) syringes of suction, be installed in respectively on the liquid feeding carriage, with the silicon rubber linking conduit I15-1 of internal diameter 2mm the interface of sealed interface on syringe, the vacuum tank 1 and the rubber seal plug 22 on the withstand voltage test tube 21 is connected, 5 test organisms sheets are put into respectively in 5 withstand voltage test tubes 21, cover rubber seal plug 22.
Requirement according to " disinfection technology standard " is made the bacterium sheet with test strain gemma suspension.Require the bacterium amount 1 * 10
6～5 * 10
6The CFU/ sheet need carry out enumeration.
2) the withstand voltage test tube 21 that the test organisms sheet will be housed is installed on the lower fixed frame 20-9 of drag proofing unit 20, clamp with following movable block 20-1, rubber seal plug 22 is used movable block 20-2 and upper holder 20-8 fixes, described silicon rubber linking conduit I15-1 is connected with micro valve 20-3.
3) with disinfectant H
2O
2In the syringe of suction sterile medium charging point 17, being installed on the liquid feeding carriage, is that the tetrafluoroethylene linking conduit II15-2 of 1.5mm is connected on the miniature electromagnetic valve 18 with internal diameter.
4) close the hermatic door 2 of experimental tank chamber 3, on testing apparatus control touch-screen, set 0～999 second grouping time, each bacterial classification is divided into 10 groups according to germicidal action time length tests every group of 5 samples.
5) open vacuum pump 7 and vacuumize, open the rubber seal plug 22 on the withstand voltage test tube 21.
6) when vacuum tightness reaches 50～150Pa, close the sealing plug of withstand voltage test tube, open plasma exciatiaon power supply 10 and carried out pre-plasma glow discharge 1～10 minute.
7) open miniature electromagnetic valve 18, add disinfectant H
2O
2And vaporization, keep diffusion in 1～10 minute.
8) sealing plug of opening withstand voltage test tube carries out bactericidal reaction and timing, and the reaction times was controlled at 0～999 second, immediately closed the sealing plug of withstand voltage test tube, open micro valve 20-3 simultaneously, make neutralizing agent submergence bacterium sheet, the time control accuracy is 0.1 second, stopped reaction.
9) be evacuated down to 50～150Pa once more, open plasma exciatiaon power supply 10, plasma discharge 1～10 minute.
10) close plasma exciatiaon power supply 10, open inflation interface 24, the inflation concora crush.
11) open hermatic door 2, take out the withstand voltage test tube of band sealing plug.
12) sterilization finishes, and gets 3 at random 5 bacterium sheet the insides and carries out the enumeration of bacterium sheet.
Enumeration is exactly to calculate the logarithmic value of organizing average survival gemma on the sample each action time.With action time is X-coordinate (X), and the logarithmic value of survival gemma is ordinate zou (Y), calculates gemma survival and the regression equation (Y=a+bX) of action time.Calculate the degree of correlation of each practical measurement value and linear regression equation,, calculate and reduce 90% gemma needed action time of D value according to the gained linear regression equation.
Set different program parameters, can carry out the drag test under multiple bacterial classification, the multiple condition.Changing other sterile medium composition of adding, can be liquid (as formaldehyde, Peracetic Acid etc.), also can be gas (oxyethane, oxygen, carbonic acid gas etc.), can carry out the sterilising effect simultaneous test after different media forms plasma body.
Claims (8)
1, a kind of microorganism resistance test apparatus that is used for verification of plasma sterilization, have vacuum tank (1) and hermatic door (2), thereby form experimental tank chamber (3), inwall in the experimental tank chamber is equipped with discharge electrode (4) and is connected with coupling tuner (9) and plasma exciatiaon power supply (10) with concentric cable by the sealing feed-in part on the tank body, and the plasma exciatiaon power supply is connected with electric apparatus control apparatus (12) again; The sidewall of described vacuum tank (1) is provided with and vacuumizes interface (6), this interface is connected with vacuum pump (7) by high vacuum flapper valve (8), the other end connecting circuit control device (12) of vacuum pump, also is furnished with inflation interface (24) on the described tank skin, this interface is connected the other end connecting circuit control device (12) of high vacuum flapper valve by high vacuum flapper valve (8) with air filter (11); Also equipped electrothermic constant temperature equipment (5) and vacuum detecting transmitter (13) on the outer side wall of described vacuum tank (1), it is characterized in that: drag proofing unit (20) is installed in the described experimental tank chamber (3) and is furnished with temperature sensor II (14-2), be connected to neutralizing agent charging point (16) and sterile medium charging point (17) in the outside of experimental tank chamber (3), described neutralizing agent liquid-adding device is communicated with the drag proofing unit by the sealed interface of linking conduit I (15-1) on the experimental tank locular wall, and described sterile medium filling apparatus (17) is connected to the dispersive nozzle (19) that is located at vacuum tank tank skin top by linking conduit II (15-2) through miniature electromagnetic valve (18).
2, the microorganism resistance test apparatus that is used for verification of plasma sterilization according to claim 1, it is characterized in that: described drag proofing unit (20) has the mechanical drive (23) that is fixed on middle part, experimental tank chamber (3), this device is by direct-current machine (23-1), wheel box (23-2), turn-screw (23-3), driving nut (23-4), motion bar (23-5), axle sleeve (23-6) and overcoat (23-7) are formed, on the overcoat (23-7) of its vertical fixing, be fixed with lower fixed frame (20-9) and movable block (20-1) down be housed at the one end, on the motion bar (23-5) of mechanical drive (23), be fixed with upper holder (20-8) and movable block (20-2) and micro valve (20-3) be housed at the one end, motion bar (23-5) outside on upper holder (20-8) is with spring (20-7) and on its top gland (20-6) is housed, and is provided with fixed briquetting (20-4) and active spill spin block (20-5) between this gland and micro valve (20-3).
3, the microorganism resistance test apparatus that is used for verification of plasma sterilization according to claim 1 and 2, it is characterized in that: described electrothermic constant temperature equipment (5) is made up of the hot plate and the thermal insulation layer that are located at vacuum tank (1) outside, and is furnished with temperature sensor and temperature controller.
4, the microorganism resistance test apparatus that is used for verification of plasma sterilization according to claim 1 and 2, it is characterized in that: described vacuum tank (1) is made by stainless steel, titanium alloy or aluminium alloy, be shaped as drum shape or rectangle, vertical or horizontal placement, volume is 30～150L.
5, the microorganism resistance test apparatus that is used for verification of plasma sterilization according to claim 1 and 2, it is characterized in that: described discharge electrode (4) is made by stainless steel, titanium alloy or aluminium alloy porous plate, with the tank skin gap be 5～50mm, and insulate with tank skin.
6, the microorganism resistance test apparatus that is used for verification of plasma sterilization according to claim 1 and 2, it is characterized in that: described neutralizing agent charging point (16) comprises 1～5 syringe and liquid feeding carriage, has the substratum that contains neutralizing agent of 5～10ml in each syringe.
7, the microorganism resistance test apparatus that is used for verification of plasma sterilization according to claim 1 and 2 is characterized in that: described sterile medium charging point (17) comprises syringe and liquid feeding carriage.
8, the microorganism resistance test apparatus that is used for verification of plasma sterilization according to claim 1 and 2 is characterized in that: near described neutralizing agent charging point (16) and the sterile medium charging point (17) temperature sensor I (14-1) is housed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201642621U CN201272809Y (en) | 2008-09-12 | 2008-09-12 | Microorganism resistance test apparatus for plasma sterilizing validation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201642621U CN201272809Y (en) | 2008-09-12 | 2008-09-12 | Microorganism resistance test apparatus for plasma sterilizing validation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201272809Y true CN201272809Y (en) | 2009-07-15 |
Family
ID=40883157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201642621U Expired - Fee Related CN201272809Y (en) | 2008-09-12 | 2008-09-12 | Microorganism resistance test apparatus for plasma sterilizing validation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201272809Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101363003B (en) * | 2008-09-12 | 2012-02-29 | 刘川生 | Microorganism resistance test apparatus and method for verification of plasma sterilization |
| CN115161166A (en) * | 2022-07-27 | 2022-10-11 | 安徽大学 | Experimental device for low temperature plasma inactivation microorganism |
-
2008
- 2008-09-12 CN CNU2008201642621U patent/CN201272809Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101363003B (en) * | 2008-09-12 | 2012-02-29 | 刘川生 | Microorganism resistance test apparatus and method for verification of plasma sterilization |
| CN115161166A (en) * | 2022-07-27 | 2022-10-11 | 安徽大学 | Experimental device for low temperature plasma inactivation microorganism |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107014426A (en) | For making the method and inline sensor device that inline sensor device puts into operation | |
| CN102350003B (en) | Hydrogen peroxide low-temperature sterilizing equipment and use method thereof | |
| CN201272809Y (en) | Microorganism resistance test apparatus for plasma sterilizing validation | |
| CN101363003B (en) | Microorganism resistance test apparatus and method for verification of plasma sterilization | |
| CN210340912U (en) | Microbial cultivation device | |
| CN203634531U (en) | Fast microbial sterilizing effect detection device | |
| US2706702A (en) | Method for culture of specimen | |
| CN214270866U (en) | Be used for clinical laboratory's inspection to cultivate bacterial culture dish with microorganism | |
| Speers et al. | Anaerobic cell culture | |
| Wolfe et al. | A vacuum-vortex technique for preparation of anoxic solutions or liquid culture media in small volumes for cultivating methanogens or other strict anaerobes | |
| CN107118959A (en) | Improved microbiological incubator | |
| CN201473542U (en) | Biologic culture box used for hydrogen peroxide plasma sterilizer | |
| CN203634532U (en) | Low-temperature plasma sterilization visualized monitoring system | |
| CN205616897U (en) | Full -automatic blood culture instrument | |
| CN202283362U (en) | Hydrogen peroxide low temperature sterilization device | |
| CN101701184A (en) | Microbe rapid detection device and detection method thereof | |
| CN102443534B (en) | Cell intermittent hypoxic simulation experiment equipment | |
| CN201581080U (en) | Resistance testing device for low temperature plasma sterilizer | |
| WO2019165231A1 (en) | Automated bioreactor with foam collector | |
| Yang et al. | Development and evaluation of the quick anaero-system-a new disposable anaerobic culture system | |
| CN219907661U (en) | Immune bacterium culture apparatus | |
| CN211179536U (en) | ATP on-line measuring instrument | |
| CN206862907U (en) | A kind of Microbiological detection of foods device | |
| Shahin et al. | Comparative evaluation of anoxomat and conventional anaerobic GasPak jar systems for the isolation of anaerobic bacteria | |
| CN208532789U (en) | A kind of anaerobe separator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090715 Termination date: 20100912 |