Living cells culture apparatus
Technical field
It is the utility model is related to a kind of biological cell culture apparatus, more particularly to a kind of for long-term observation under the microscope
The culture apparatus of active state in cell.
Background technology
Currently, the movement for observing molecule in living cells under high magnification microscope has been indispensable in cell biology
Step, this technology are mutual between the observation and cell of the protein labeling tracking of cell biology, cellular morphology variation
Effect provides valuable real time information, has quickly pushed the development of cell biology.But, it is generally the case that it is high-end micro-
Mirror can just give the cell incubator prepared and nearby used dedicated for microscope carrier.Such cell incubator can only be adapted to
Specific microscope type;Such cell incubator volume is bigger than normal, complicated characteristic so that its nothing on simple microscope
Method close friend is adapted to.The installation and removal of such cell incubator are all extremely complex, though have in laboratory more adaptation it is micro-
Mirror, incubator can not also move general between each microscope.Currently, most of scientific research institutions and laboratory be equipped with it is micro-
Mirror can not be compatible with incubator, and be adapted to the microscope of incubator and its limited and sufficiently expensive, so leading to big portion at home
Point R&D institution can not all be observed living cells using microscope in the environment of a suitable cells survival.
Invention content
The goal of the invention of the utility model be in order to overcome the shortcomings of that background technology is paid, provide it is a kind of it is simple in structure, at
This relatively low, preferable living cells culture apparatus of versatility.
The technical solution of the utility model is:A kind of living cells culture apparatus, including Tissue Culture Dish, Tissue Culture Dish are logical
The container I that pipeline connection one fills distilled water is crossed, heating device is additionally provided on container I, container I also has appearance by pipeline connection
Device II, container II are connected to air pump and carbon dioxide air source respectively by pipeline.
The utility model has the beneficial effects that:When carrying out the experiment of the micro- sem observation of living cells, Tissue Culture Dish is put
It is placed under microscope, air is passed into heating and moistening in container I after being mixed in container II with carbon dioxide, be then directed to just
Under the microscope in observed Tissue Culture Dish, required temperature, humidity and nutrient are provided for cell.It is being not equipped with
Cell incubator is not adapted to the condition that living cells observation is provided under the microscope of cell incubator, greatly reduces experimental cost,
Improve quality of experiments.And use can be moved between more microscopes, greatly improve the flexibility of experiment.
Description of the drawings
The utility model is further illustrated with reference to the accompanying drawings and examples.
Fig. 1 is the structural schematic diagram of the utility model;
Wherein:1, Tissue Culture Dish, 2, ware lid with holes, 3,7,11,9, hose, 4, container I, 5, distilled water, 6, thermostatted water
Bath, 8, container II, 10, flow velocity valve I, 12, flow velocity valve II, 13, carbon dioxide air source, 14, air pump.
Specific implementation mode
The present embodiment as shown in Figure 1, a kind of living cells culture apparatus, including Tissue Culture Dish(1), Tissue Culture Dish
(1)It is equipped with ware lid with holes(2), in ware lid with holes(2)On pass through hose(3)It is communicated with container I(4), container I is equipped with steaming
Distilled water(5)Conical flask.
Container I(4)It is arranged in water bath with thermostatic control, the temperature range of water bath with thermostatic control is 35-40 DEG C, preferably 37 DEG C.
There are two intercommunicating pores, one of intercommunicating pore to pass through hose for setting on the conical flask bottle stopper of container I(3)With culture dish
Connection, another intercommunicating pore pass through hose(7)It is communicated with container II(8).
The container II(8)For the conical flask for setting there are three intercommunicating pore, first through-hole passes through hose(7)With container I
(4)Connection;Second through-hole passes through hose(9)Access air pump(14), in hose(9)On be provided with flow velocity valve I(10);Third
A through-hole passes through hose(11)Access carbon dioxide air source(13), in hose(11)On be provided with flow velocity valve II(12).
The distilled water(5)For the medical distilled water of this field routine, pH value is between 6.0-7.0, conductivity 0.4-
0.6US/CM, 10-15 megaohms of resistivity/CM.
In test, by the Tissue Culture Dish equipped with living cells(1)It is placed on microscopical objective table, is starting microscope
Before observation and record, air pump is opened respectively(14)And carbon dioxide air source(13), flow velocity valve I(10)With flow velocity valve II(12)
The velocity ratio for adjusting air and carbon dioxide respectively is 95:5.5% carbon dioxide and 95% air are in container II(8)In fill
After dividing mixing, mixed gas keeps 5 m of general gas flow3The speed of/s enters container I(4).In container I(4)In, mixed gas obtains
Sufficient humidity has been arrived, and has been heated to 37 degrees centigrades, from hose(3)It is passed directly to Tissue Culture Dish(1)In, to tie up
Hold health and the survival of living cells.After the system of waiting for settles out, experimenter can begin to use micro- sem observation culture dish
In living cells.