CN207713752U - Living cells culture apparatus - Google Patents
Living cells culture apparatus Download PDFInfo
- Publication number
- CN207713752U CN207713752U CN201721779597.XU CN201721779597U CN207713752U CN 207713752 U CN207713752 U CN 207713752U CN 201721779597 U CN201721779597 U CN 201721779597U CN 207713752 U CN207713752 U CN 207713752U
- Authority
- CN
- China
- Prior art keywords
- container
- living cells
- culture dish
- tissue culture
- culture apparatus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of living cells culture apparatus, including Tissue Culture Dish, Tissue Culture Dish fills the container I of distilled water by pipeline connection one, it is additionally provided with heating device on container I, container I also has container II, container II to be connected to respectively with air pump and carbon dioxide air source by pipeline by pipeline connection.When carrying out the experiment of the micro- sem observation of living cells, Tissue Culture Dish is positioned under microscope, air is passed into heating and moistening in container I after being mixed in container II with carbon dioxide, then it is directed in the Tissue Culture Dish being just observed under the microscope, required temperature, humidity and nutrient is provided for cell.
Description
Technical field
It is the utility model is related to a kind of biological cell culture apparatus, more particularly to a kind of for long-term observation under the microscope
The culture apparatus of active state in cell.
Background technology
Currently, the movement for observing molecule in living cells under high magnification microscope has been indispensable in cell biology
Step, this technology are mutual between the observation and cell of the protein labeling tracking of cell biology, cellular morphology variation
Effect provides valuable real time information, has quickly pushed the development of cell biology.But, it is generally the case that it is high-end micro-
Mirror can just give the cell incubator prepared and nearby used dedicated for microscope carrier.Such cell incubator can only be adapted to
Specific microscope type；Such cell incubator volume is bigger than normal, complicated characteristic so that its nothing on simple microscope
Method close friend is adapted to.The installation and removal of such cell incubator are all extremely complex, though have in laboratory more adaptation it is micro-
Mirror, incubator can not also move general between each microscope.Currently, most of scientific research institutions and laboratory be equipped with it is micro-
Mirror can not be compatible with incubator, and be adapted to the microscope of incubator and its limited and sufficiently expensive, so leading to big portion at home
Point R＆D institution can not all be observed living cells using microscope in the environment of a suitable cells survival.
Invention content
The goal of the invention of the utility model be in order to overcome the shortcomings of that background technology is paid, provide it is a kind of it is simple in structure, at
This relatively low, preferable living cells culture apparatus of versatility.
The technical solution of the utility model is：A kind of living cells culture apparatus, including Tissue Culture Dish, Tissue Culture Dish are logical
The container I that pipeline connection one fills distilled water is crossed, heating device is additionally provided on container I, container I also has appearance by pipeline connection
Device II, container II are connected to air pump and carbon dioxide air source respectively by pipeline.
The utility model has the beneficial effects that：When carrying out the experiment of the micro- sem observation of living cells, Tissue Culture Dish is put
It is placed under microscope, air is passed into heating and moistening in container I after being mixed in container II with carbon dioxide, be then directed to just
Under the microscope in observed Tissue Culture Dish, required temperature, humidity and nutrient are provided for cell.It is being not equipped with
Cell incubator is not adapted to the condition that living cells observation is provided under the microscope of cell incubator, greatly reduces experimental cost,
Improve quality of experiments.And use can be moved between more microscopes, greatly improve the flexibility of experiment.
Description of the drawings
The utility model is further illustrated with reference to the accompanying drawings and examples.
Fig. 1 is the structural schematic diagram of the utility model；
Wherein：1, Tissue Culture Dish, 2, ware lid with holes, 3,7,11,9, hose, 4, container I, 5, distilled water, 6, thermostatted water
Bath, 8, container II, 10, flow velocity valve I, 12, flow velocity valve II, 13, carbon dioxide air source, 14, air pump.
Specific implementation mode
The present embodiment as shown in Figure 1, a kind of living cells culture apparatus, including Tissue Culture Dish（1）, Tissue Culture Dish
（1）It is equipped with ware lid with holes（2）, in ware lid with holes（2）On pass through hose（3）It is communicated with container I（4）, container I is equipped with steaming
Distilled water（5）Conical flask.
Container I（4）It is arranged in water bath with thermostatic control, the temperature range of water bath with thermostatic control is 35-40 DEG C, preferably 37 DEG C.
There are two intercommunicating pores, one of intercommunicating pore to pass through hose for setting on the conical flask bottle stopper of container I（3）With culture dish
Connection, another intercommunicating pore pass through hose（7）It is communicated with container II（8）.
The container II（8）For the conical flask for setting there are three intercommunicating pore, first through-hole passes through hose（7）With container I
（4）Connection；Second through-hole passes through hose（9）Access air pump（14）, in hose（9）On be provided with flow velocity valve I（10）；Third
A through-hole passes through hose（11）Access carbon dioxide air source（13）, in hose（11）On be provided with flow velocity valve II（12）.
The distilled water（5）For the medical distilled water of this field routine, pH value is between 6.0-7.0, conductivity 0.4-
0.6US/CM, 10-15 megaohms of resistivity/CM.
In test, by the Tissue Culture Dish equipped with living cells（1）It is placed on microscopical objective table, is starting microscope
Before observation and record, air pump is opened respectively（14）And carbon dioxide air source（13）, flow velocity valve I（10）With flow velocity valve II（12）
The velocity ratio for adjusting air and carbon dioxide respectively is 95：5.5% carbon dioxide and 95% air are in container II（8）In fill
After dividing mixing, mixed gas keeps 5 m of general gas flow3The speed of/s enters container I（4）.In container I（4）In, mixed gas obtains
Sufficient humidity has been arrived, and has been heated to 37 degrees centigrades, from hose（3）It is passed directly to Tissue Culture Dish（1）In, to tie up
Hold health and the survival of living cells.After the system of waiting for settles out, experimenter can begin to use micro- sem observation culture dish
In living cells.
Claims (4)
1. a kind of living cells culture apparatus, including Tissue Culture Dish, it is characterized in that Tissue Culture Dish is filled by pipeline connection one
The container I of distilled water is additionally provided with heating device on container I, and container I also has container II, container II to pass through by pipeline connection
Pipeline is connected to air pump and carbon dioxide air source respectively.
2. living cells culture apparatus as described in claim 1, it is characterized in that the heating device is water bath with thermostatic control, it is described
Container I is positioned in water bath with thermostatic control, and the temperature of the water bath with thermostatic control is maintained at 35-40 DEG C.
3. living cells culture apparatus as described in claim 1, it is characterized in that the pipeline between the container II and air pump
It is equipped with flow velocity valve I, the pipeline of the container II and carbon dioxide air source is equipped with flow velocity valve II.
4. living cells culture apparatus as claimed in claim 3, it is characterized in that the stream between the flow velocity valve I and flow velocity valve II
Speed ratio is 95：5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721779597.XU CN207713752U (en) | 2017-12-19 | 2017-12-19 | Living cells culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721779597.XU CN207713752U (en) | 2017-12-19 | 2017-12-19 | Living cells culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207713752U true CN207713752U (en) | 2018-08-10 |
Family
ID=63056354
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721779597.XU Expired - Fee Related CN207713752U (en) | 2017-12-19 | 2017-12-19 | Living cells culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207713752U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113820843A (en) * | 2021-07-22 | 2021-12-21 | 西安电子科技大学 | High-resolution quantitative phase microscope system based on annular LED illumination |
-
2017
- 2017-12-19 CN CN201721779597.XU patent/CN207713752U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113820843A (en) * | 2021-07-22 | 2021-12-21 | 西安电子科技大学 | High-resolution quantitative phase microscope system based on annular LED illumination |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101985600B (en) | Turnover membrane bioreactor for use in micro-gravity environment | |
| CN104321419A (en) | Cell culture system and cell culture method | |
| CN106318866A (en) | Small multi-purpose cell culturing and testing device | |
| CN106520552B (en) | A kind of biological reactor for cell culture | |
| US20130260450A1 (en) | Bioreactor with lid for easy access to incubation cavity | |
| CN113583939A (en) | Construction method of model for crossing blood brain barrier by adeno-associated virus | |
| CN207713752U (en) | Living cells culture apparatus | |
| US20180291325A1 (en) | In vitro pharmacokinetic-pharmacodynamic device | |
| CN106367346A (en) | Mesenchymal stem cell extraction and perfusion culture system | |
| CN103320494A (en) | Method for measuring invasiveness of multiple cells to cell matrix embedded with endothelial cells by utilizing Transwell | |
| CN103146636A (en) | Hiberarchy microcarrier and preparation method and application thereof | |
| CN101724557B (en) | Polysaccharide composite stent perfusion type liver cell reactor system for use in medicament-induced hepatotoxicity evaluation | |
| CN106635796B (en) | Device and fixed cultural method for cell fixation culture | |
| CN206020212U (en) | A kind of BOD online auto monitoring systems | |
| CN105754856B (en) | A kind of drawer type three-dimensional class blood vessel tissue culture organ chip and its construction method | |
| CN206940914U (en) | Hepatocyte heterogeneity culture apparatus | |
| CN102010822A (en) | Cell culture fluid atomizing supply device | |
| CN101597560A (en) | A kind of device that is used for preparing plating medium | |
| CN113736658B (en) | Long-term large-scale amplification biological reaction system for human umbilical cord mesenchymal stem cells | |
| CN205839028U (en) | A kind of automatic cytological culture apparatus for differentiation of neural stem cells | |
| Pörtner et al. | Cultivation of mammalian cells in fixed-bed reactors | |
| CN109294909A (en) | A kind of cell observation incubator | |
| RU2626526C1 (en) | System of animal and human tissue bio-engineering models creation | |
| CN203683552U (en) | Plate cover structure of cell culture plate | |
| CN102485878B (en) | Multi-cell co-culture apparatus used in simulated weightless environment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210810 Address after: 215000 unit 101b, building 20, zone B, phase II, biomedical industrial park, No. 218, Sangtian street, Suzhou Industrial Park, Jiangsu Province Patentee after: Suzhou Jingrui Biotechnology Co.,Ltd. Address before: 215000 unit 510, building A4, biological nano Park, 218 Xinghu street, industrial park, Suzhou, Jiangsu Province Patentee before: CURE GENETICS Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180810 Termination date: 20211219 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |