CN205839028U - A kind of automatic cytological culture apparatus for differentiation of neural stem cells - Google Patents
A kind of automatic cytological culture apparatus for differentiation of neural stem cells Download PDFInfo
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- CN205839028U CN205839028U CN201620417538.7U CN201620417538U CN205839028U CN 205839028 U CN205839028 U CN 205839028U CN 201620417538 U CN201620417538 U CN 201620417538U CN 205839028 U CN205839028 U CN 205839028U
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Abstract
This utility model provides a kind of automatic cytological culture apparatus for differentiation of neural stem cells, including culture tank, nutritional solution storage tank, metabolism waste collection tank, cell culture sample storage tank and cell collecting tank, described culture tank top is provided with cultivation cell outlet, waste liquid outlet and stromal cell outlet, it is provided with cell sample entrance, nutritional solution entrance and stromal cell entrance bottom described culture tank, in described culture tank, is provided with nutrition transmission pipe fitting, spiral cell sample transfer tube and metabolic waste recovery tube.The device that this utility model provides is effectively increased culture efficiency, good culture environment is improve for neural stem cell differentiating cell, Intelligent control, culture efficiency is higher, and the survival rate of noble cells and qualification rate are higher, Intelligent Measurement can be accomplished to cultivating cell, be effectively improved culture effect.
Description
Technical field
This utility model relates to technical field of cell culture, particularly to a kind of for differentiation of neural stem cells from
Dynamicization cell culture system.
Background technology
Stem cell refers to the germinal cell with self renewal and multi-lineage potential, is the cells of origin of body, spy
Can break up under conditions of Ding and become one or more composition tissue or cells of organ.Self renewal refers to that stem cell can
The size of stem cell population is maintained by cell division；Multidirectional Differentiation refers to that stem cell has plasticity, in different environments,
Different histiocytes can be divided into thus constitute the histoorgan of the various complexity of body.Either cell therapy is still organized
Engineering is required for a considerable amount of stem cell, but source of human stem cell is limited, and the plane such as currently common porous plate, square vase cultivates behaviour
Making complexity, growth microenvironment discontinuity changes huge, and cell growth impact is big, and the cell number of results does not reaches the most far away and faces
Bed needs.Meanwhile, directly adding combination of cytokines in cultivating system also can make toxigenic capacity be greatly increased, it is difficult to will be dry thin
Clinical practice is pushed in born of the same parents' treatment to.Therefore, suddenly need one can a large amount of culturing stem cells rapidly in vitro, and will not significantly increase
The device adding toxigenic capacity comes out.
Neural stem cell (Neural Stem Cell, NSCs) is the one of stem cell, and neural stem cell is at neurodevelopment
Play a significant role with repairing in damaged nerve tissue.Neural stem cells transplantation be repair and replace injured cerebral tissue have efficacious prescriptions
Method, can rebuild partial loop and function.In addition neural stem cell can be as genophore, for intracranial tumor and other neural disease
Sick gene therapy, utilizes neural stem cell as gene therapy vector, compensate for the some shortcomings of viral vector.Additionally, it is refreshing
It is alternatively arranged as a kind of cell model to evaluate toxicity and the pharmacological action of new drug through stem cell.In order to meet neural stem cell
Demand, is that particular cell types is (such as neurocyte, muscle cell, islets of langerhans by differentiation of neural stem cells in prior art
Element secretory cell, osteoblast, adipose cell etc.) method in, mostly be interpolation external source chemical reagent, combination of cytokines is sought
Make the microenvironment of stem cell directional differentiation, while toxigenic capacity does not descends according to height, there is also various factor agents and consume in time
And occur in different time points and there is Concentraton gradient, inducible system such as is not sufficiently stable at the shortcoming, and cell survival rate is low simultaneously, stability
Difference, culture efficiency is relatively slow, therefore, is badly in need of a kind of suitable automatic cytological that can be used in differentiation of neural stem cells and cultivates
Device.
Utility model content
In order to solve drawbacks described above present in prior art, this utility model provides and a kind of lures for neural stem cell
Lead the automatic cytological culture apparatus of differentiation.
The concrete technical scheme of this utility model is as follows:
This utility model provides a kind of automatic cytological culture apparatus for differentiation of neural stem cells, including training
Support tank, nutritional solution storage tank, metabolism waste collection tank, cell culture sample storage tank and cell collecting tank,
Described culture tank top is provided with cultivation cell outlet, waste liquid outlet and stromal cell outlet, bottom described culture tank
It is provided with cell sample entrance, nutritional solution entrance and stromal cell entrance, in described culture tank, is provided with nutrition transmission pipe fitting, spiral
Cell sample transfer tube and metabolic waste recovery tube, described nutrition transmission pipe fitting is connected with described nutritional solution entrance；Described metabolism
Waste recovery pipe one end is connected with described waste liquid outlet, and its other end is closed；Described spiral cell sample transfer tube is along described
Culture tank inwall axial-rotation is distributed, and described spiral cell sample transfer tube one end is connected with described cell sample entrance, its
The other end is connected with described cultivation cell outlet, is filled with stromal cell, attached cell or direct growth in described culture tank
Suspension cell；
Described nutritional solution storage tank is connected with the described nutritional solution entrance of described culture tank by the first transmission pipeline, institute
State the first transmission pipeline and be provided with the first transmission pump；Described metabolism waste collection tank is by the second transmission pipeline and described culture tank
Described waste liquid outlet connect, described second transmission pipeline is provided with the first draw-off pump；Described cell culture sample storage tank leads to
Crossing the 3rd transmission pipeline to be connected with the described cell sample entrance of described culture tank, described 3rd transmission pipeline is provided with the second biography
Defeated pump；Described cell collecting tank is connected with the described cultivation cell outlet of described culture tank by the 4th transmission pipeline, and described the
Four transmission pipelines are provided with the second draw-off pump.
Further, described nutrition transmission pipe fitting includes being positioned at the ring pipe bottom described culture tank, on described ring pipe
Connection has some along the axially extended arm of described culture tank, and described ring pipe bottom side is connected with described nutritional solution entrance,
Described arm one end connects with described ring pipe, and its other end is closed, and the tube wall of described ring pipe and described arm is semi-transparent
Film.
Further, this culture apparatus also includes that intelligent control system, described intelligent control system include controller and be arranged on described
Sensor cluster in culture tank, described sensor cluster, described first transmission pump, described first draw-off pump, described second biography
Defeated pump, described second draw-off pump are all connected with described controller；
Temperature sensor that described sensor cluster includes communicating with described controller, pH sensor, pressure transducer
And dissolved oxygen sensor.
Further, described second transmission pipeline is provided with waste liquid detector, described waste liquid detector and described controller
Communicate.
Further, described 4th transmission pipeline is provided with three-way magnetic valve, and described 4th transmission pipeline is by described threeway
Electromagnetic valve connects circulating line, and described circulating line connects with described 3rd transmission pipeline away from one end of described three-way magnetic valve
Logical, described three-way magnetic valve is provided with cell detection instrument, described three-way magnetic valve, described cell detection instrument all with described controller
Connect.
Further, on described 3rd transmission pipeline, one end of close described cell culture sample storage tank is provided with described
The check valve that controller communicates.
Preferably, the tube wall of described spiral cell sample transfer tube and described metabolic waste recovery tube is semipermeable membrane.
The beneficial effects of the utility model are as follows: the device that this utility model provides is effectively increased culture efficiency, for god
The cell broken up through stem cell improves good culture environment, Intelligent control, and culture efficiency is higher, and the one-tenth of noble cells
Motility rate and qualification rate are higher, it is possible to accomplish Intelligent Measurement to cultivating cell, be effectively improved culture effect.
Accompanying drawing explanation
Fig. 1 is the knot of a kind of automatic cytological culture apparatus for differentiation of neural stem cells described in embodiment 1
Composition；
Fig. 2 is a kind of battalion in the automatic cytological culture apparatus of differentiation of neural stem cells described in embodiment 2
Support the structure chart of transmission pipe fitting；
Fig. 3 is the knot of a kind of automatic cytological culture apparatus for differentiation of neural stem cells described in embodiment 2
Composition.
Wherein: 1, culture tank；101, cell outlet is cultivated；102, waste liquid outlet；103, stromal cell outlet；105, cell
Sample entrance port；106, nutritional solution entrance；107, stromal cell entrance；108, spiral cell sample transfer tube；109, metabolism is given up
Thing recovery tube；2, nutritional solution storage tank；3, metabolism waste collection tank；4, cell culture sample storage tank；5, cell collecting tank；6、
First transmission pipeline；7, the first transmission pump；8, the second transmission pipeline；9, the first draw-off pump；10, the 3rd transmission pipeline；11, second
Transmission pump；12, the 4th transmission pipeline；13, the second draw-off pump；14, ring pipe；15, arm；16, controller；17, waste liquid detection
Instrument；18, three-way magnetic valve；19, circulating line；20, cell detection instrument；21, check valve.
Detailed description of the invention
With following example, this utility model is described in further detail below in conjunction with the accompanying drawings.
Embodiment 1
As it is shown in figure 1, this utility model embodiment 1 to provide a kind of automatization for differentiation of neural stem cells thin
Born of the same parents' culture apparatus, including culture tank 1, nutritional solution storage tank 2, metabolism waste collection tank 3, cell culture sample storage tank 4 and thin
Born of the same parents' collecting tank 5,
Culture tank 1 is cultivated for the automatization of differentiation of neural stem cells, and nutritional solution storage tank 2 is used for storing nutrition
Liquid, metabolism waste collection tank 3 is used for storing metabolism waste liquid, and cell culture sample storage tank 4 is received for torage cell sample, cell
The collection tank 5 cell after storing cultivation.
Described culture tank 1 top is provided with cultivation cell outlet 101, waste liquid outlet 102 and stromal cell outlet 103, described
It is provided with cell sample entrance 105, nutritional solution entrance 106 and stromal cell entrance 107 bottom culture tank 1, sets in described culture tank 1
Nutritious transmission pipe fitting, spiral cell sample transfer tube 108 and metabolic waste recovery tube 109, described nutrition transmission pipe fitting with
Described nutritional solution entrance 106 connects；Described metabolic waste recovery tube 109 one end is connected with described waste liquid outlet 102, its other end
Close；Described spiral cell sample transfer tube 108 is distributed along described culture tank 1 inwall axial-rotation, described spiral cell
Sample delivery pipe 108 one end is connected with described cell sample entrance 105, and its other end is connected with described cultivation cell outlet 101,
The suspension cell of stromal cell, attached cell or direct growth it is filled with in described culture tank 1.
Cell sample can be more fed through in culture tank 1 by spiral cell sample transfer tube 108 fully to be trained
Support so that it is fully absorb the nutrition in culture tank 1, improve culture efficiency, be trained motility rate higher.Additionally, passed by nutrition
Defeated pipe fitting can provide sufficient nutrient substance for the cell in culture tank 1.
Described nutritional solution storage tank 2 is connected by the described nutritional solution entrance 106 of the first transmission pipeline 6 with described culture tank 1
Connecing, described first transmission pipeline 6 is provided with the first transmission pump 7；Described metabolism waste collection tank 3 by the second transmission pipeline 8 with
The described waste liquid outlet 102 of described culture tank 1 connects, and described second transmission pipeline 8 is provided with the first draw-off pump 9；Described cell
Culture sample storage tank 4 is connected with the described cell sample entrance 105 of described culture tank 1 by the 3rd transmission pipeline 10, described
3rd transmission pipeline 10 is provided with the second transmission pump 11；Described cell collecting tank 5 is by the 4th transmission pipeline 12 and described cultivation
The described cultivation cell outlet 101 of tank 1 connects, and described 4th transmission pipeline 12 is provided with the second draw-off pump 13.
Can regulate the pressure in culture tube by draw-off pump and transmission pump, cultivating for cell provides a stable cultivation
Environment.
Embodiment 2
This utility model embodiment 2 provides a kind of automatic cytological for differentiation of neural stem cells and cultivates dress
Putting, this embodiment 2 further defines the structure of culture apparatus on the basis of embodiment 1, practical.
As in figure 2 it is shown, the nutrition providing sufficient to give cell to cultivate, the technical program further defines institute
Stating nutrition transmission pipe fitting and include being positioned at the ring pipe 14 bottom described culture tank 1, on described ring pipe 14, connection has some along institute
Stating the axially extended arm of culture tank 1 15, described ring pipe 14 bottom side is connected with described nutritional solution entrance 106, described
Pipe 15 one end connects with described ring pipe 14, and its other end is closed, and the tube wall of described ring pipe 14 and described arm 15 is half
Permeable membrane.Ring pipe 14 is connected with nutritional solution entrance 106 and is passed through nutritional solution, and the nutritional solution in ring pipe 14 is passed through arm 15 again
In, it is ensured that nutritional labeling even density in culture tank 1, the nutrition providing sufficient is cultivated for cell.
As it is shown on figure 3, for the culture technique improving automatization, the technical program further defines, this cultivation
Device also includes that intelligent control system, described intelligent control system include controller 16 and the sensor cluster being arranged in described culture tank 1,
Described sensor cluster, described first transmission pump 7, described first draw-off pump 9, described second transmission pump 11, described second extraction
Pump 13 is all connected with described controller 16；By controller 16 can effectively control first transmission pump the 7, second transmission pump 11,
Being turned on and off of one draw-off pump 9 and the second draw-off pump 13, it might even be possible to for the size of Intelligent adjustment pump.
Temperature sensor that described sensor cluster includes communicating with described controller 16, pH sensor, pressure sensing
Device and dissolved oxygen sensor.The temperature in culture tank 1 can be effectively monitored in temperature sensor, and is transferred to controller 16, for carefully
Born of the same parents cultivate and provide suitable temperature.PH sensor, for detecting the pH value in culture tank 1, is effectively cultivated for cell and is provided suitable
PH value.Pressure transducer, for detecting the pressure in culture tank 1, provides pressure slightly above first to take out by the first transmission pump 7
Take pump 9 swabbing pressure, combine the second transmission pump 11 and coordination of the second draw-off pump 13 simultaneously, the intrinsic pressure holding of culture tank 1 can be made slightly
Superatmospheric, while preventing seepage and external source from polluting, makes part pot liquid be discharged by cell culture tube, can fully protect
The cytokine that card stromal cell secretion provides can abundant and purpose cytosis.
Described second transmission pipeline 8 is provided with waste liquid detector 17, described waste liquid detector 17 and described controller 16 phase
Communication.By its detection to metabolism waste liquid, it is adjusted the feed speed of the first transmission pump 7, keeps the steady of the interior microenvironment of pipe
Fixed；Also by the waste liquid detector 17 detection to metabolism waste liquid, it is judged that its residue nutrient number, when residue nutrient fewer
Time, then the nutritional solution in nutritional solution storage tank 2 can carry out supplementing nutrient, or all, partly change nutritional solution storage tank 2
In nutritional solution, it is ensured that the nutritional solution nutrient entering the first transmission pipeline 6 is sufficient.This waste liquid detector 17 can be existing inspection
Surveying device or detection chip, it can directly be buied from market, does not do too much repeating at this.
Described 4th transmission pipeline 12 is provided with three-way magnetic valve 18, and described 4th transmission pipeline 12 is by described threeway electromagnetism
Valve 18 connects circulating line 19, and described circulating line 19 is away from one end and described 3rd transmission pipeline of described three-way magnetic valve 18
10 connections, described three-way magnetic valve 18 is provided with cell detection instrument 20, and described three-way magnetic valve 18, described cell detection instrument 20 are equal
It is connected with described controller 16.As required, it is also possible between the second draw-off pump 13 and cell collecting tank 5, set up a pair cell
Size, endochylema endoparticle situation, cell proportion etc. carry out the cell detection instrument 20 detected, and cell detection instrument 20 can be a control
Valve processed, be in this utility model limit three-way magnetic valve 18, when cell detection instrument 20 detect cell qualified time, then by cell
Import in cell collecting tank 5, when cell detection instrument 20 detect cell defective time, then cell is directed back to by circulating line 19
In 3rd transmission pipeline 10 so that it is be again introduced into culture tank 1.
For preventing cell from flowing backwards in cell culture sample storage tank 4, in the technical program, define described 3rd transmission
On pipeline 10, the one end near described cell culture sample storage tank 4 is provided with the check valve 21 communicated with described controller 16.
Preferably devising in the technical program, described spiral cell sample transfer tube 108 and described metabolic waste return
The tube wall of closed tube 109 is semipermeable membrane.Described semipermeable membrane be designed to ensure the normal nutrition supply of cell, simultaneously can make training
The nutrient substance such as the cytokine that in foster tank 1, stromal cell secretion provides can enter spiral cell sample transfer tube 108
In, cell sample is played a role.
This utility model is not limited to above-mentioned preferred forms, and anyone can draw under enlightenment of the present utility model
Other various forms of products, no matter but in its shape or structure, make any change, every have same as the present application or phase
The technical scheme of approximation, within all falling within protection domain of the present utility model.
Claims (7)
1. the automatic cytological culture apparatus for differentiation of neural stem cells, it is characterised in that include culture tank
(1), nutritional solution storage tank (2), metabolism waste collection tank (3), cell culture sample storage tank (4) and cell collecting tank (5),
Described culture tank (1) top is provided with cultivation cell outlet (101), waste liquid outlet (102) and stromal cell outlet (103),
Described culture tank (1) bottom is provided with cell sample entrance (105), nutritional solution entrance (106) and stromal cell entrance (107), institute
Nutrition transmission pipe fitting, spiral cell sample transfer tube (108) and metabolic waste recovery tube (109) it is provided with in stating culture tank (1),
Described nutrition transmission pipe fitting is connected with described nutritional solution entrance (106)；Described metabolic waste recovery tube (109) one end is useless with described
Liquid outlet (102) connects, and its other end is closed；Described spiral cell sample transfer tube (108) is along described culture tank (1) inwall
Axial-rotation is distributed, and described spiral cell sample transfer tube (108) one end is connected with described cell sample entrance (105), its
The other end is connected with described cultivation cell outlet (101), is filled with stromal cell, attached cell or straight in described culture tank (1)
Deliver a child long suspension cell；
Described nutritional solution storage tank (2) is by the described nutritional solution entrance of the first transmission pipeline (6) with described culture tank (1)
(106) connecting, described first transmission pipeline (6) is provided with the first transmission pump (7)；Described metabolism waste collection tank (3) is by the
Two transmission pipelines (8) are connected with the described waste liquid outlet (102) of described culture tank (1), and described second transmission pipeline (8) is provided with
First draw-off pump (9)；Described cell culture sample storage tank (4) is by the 3rd transmission pipeline (10) and described culture tank (1)
Described cell sample entrance (105) connects, and described 3rd transmission pipeline (10) is provided with the second transmission pump (11)；Described cell is received
Collection tank (5) is connected with the described cultivation cell outlet (101) of described culture tank (1) by the 4th transmission pipeline (12), and described the
Four transmission pipelines (12) are provided with the second draw-off pump (13).
2. automatic cytological culture apparatus as claimed in claim 1, it is characterised in that described nutrition transmission pipe fitting includes being positioned at
The ring pipe (14) of described culture tank (1) bottom, the upper connection of described ring pipe (14) has some axially prolongs along described culture tank (1)
The arm (15) stretched, described ring pipe (14) bottom side is connected with described nutritional solution entrance (106), described arm (15) one end
Connecting with described ring pipe (14), its other end is closed, and the tube wall of described ring pipe (14) and described arm (15) is semi-transparent
Film.
3. automatic cytological culture apparatus as claimed in claim 1, it is characterised in that this culture apparatus also includes intelligence control system
System, described intelligent control system includes controller (16) and the sensor cluster being arranged in described culture tank (1), described sensor group
Part, described first transmission pump (7), described first draw-off pump (9), described second transmission pump (11), described second draw-off pump (13)
All it is connected with described controller (16)；
Temperature sensor that described sensor cluster includes communicating with described controller (16), pH sensor, pressure transducer
And dissolved oxygen sensor.
4. automatic cytological culture apparatus as claimed in claim 3, it is characterised in that set on described second transmission pipeline (8)
Waste liquid detector (17), described waste liquid detector (17) is had to communicate with described controller (16).
5. automatic cytological culture apparatus as claimed in claim 3, it is characterised in that described 4th transmission pipeline (12) is provided with
Three-way magnetic valve (18), described 4th transmission pipeline (12) connects circulating line (19), institute by described three-way magnetic valve (18)
State circulating line (19) to connect with described 3rd transmission pipeline (10) away from one end of described three-way magnetic valve (18), described threeway
Electromagnetic valve (18) is provided with cell detection instrument (20), and described three-way magnetic valve (18), described cell detection instrument (20) are all with described
Controller (16) connects.
6. automatic cytological culture apparatus as claimed in claim 5, it is characterised in that lean on described 3rd transmission pipeline (10)
One end of nearly described cell culture sample storage tank (4) is provided with the check valve (21) communicated with described controller (16).
7. automatic cytological culture apparatus as claimed in claim 1, it is characterised in that described spiral cell sample transfer tube
And the tube wall of described metabolic waste recovery tube (109) is semipermeable membrane (108).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620417538.7U CN205839028U (en) | 2016-05-09 | 2016-05-09 | A kind of automatic cytological culture apparatus for differentiation of neural stem cells |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620417538.7U CN205839028U (en) | 2016-05-09 | 2016-05-09 | A kind of automatic cytological culture apparatus for differentiation of neural stem cells |
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| CN205839028U true CN205839028U (en) | 2016-12-28 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109471471A (en) * | 2018-11-06 | 2019-03-15 | 山东中锐科技发展有限公司 | PLC self-control frequency-variable temperature controlling instruments applied to artificial environment |
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2016
- 2016-05-09 CN CN201620417538.7U patent/CN205839028U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109471471A (en) * | 2018-11-06 | 2019-03-15 | 山东中锐科技发展有限公司 | PLC self-control frequency-variable temperature controlling instruments applied to artificial environment |
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