CN216955410U

CN216955410U - Device for extracting apoplast body fluid from plant root system

Info

Publication number: CN216955410U
Application number: CN202220123879.9U
Authority: CN
Inventors: 杨继硕; 王云鹏; 邓晟; 魏利辉; 王楠; 李思雨; 滕辉
Original assignee: Huaiyin Institute of Technology; Jiangsu Academy of Agricultural Sciences
Current assignee: Huaiyin Institute of Technology; Jiangsu Academy of Agricultural Sciences
Priority date: 2022-01-18
Filing date: 2022-01-18
Publication date: 2022-07-12
Anticipated expiration: 2032-01-18

Abstract

The utility model discloses a device for extracting apoplasm body fluid from a plant root system, which comprises a pipe body, a collection bin, an upper cover, a separation net and an opening and closing mechanism, wherein the collection bin is arranged on the pipe body; the collecting bin is detachably connected to the bottom end of the pipe body, and the upper cover is clamped at the top of the pipe body; the separation net is detachably connected inside the pipe body; the opening and closing mechanism comprises a claw, a knob and a ratchet wheel; the clamping jaw is hinged to the pipe body, an elastic piece is connected between the clamping jaw and the pipe body, the knob is rotatably connected to the outer wall of the pipe body, and the ratchet wheel is connected to the knob; the clamping jaws are clamped on the upper cover so that the upper cover is tightly buckled on the top end of the pipe body; when the ratchet wheel rotates, the claw is pushed to be separated from the upper cover; the outer wall of the top end of the tube body is provided with an annular step groove, and the bottom end of the upper cover is clamped in the step groove; the upper cover is connected with the pipe body through a hinge. The utility model is provided with the separation net, and the root system is placed on the separation net, thereby avoiding the trouble that solid and liquid need to be separated after centrifugation, and obtaining clean and pollution-free extraplasmic body fluid.

Description

Device for extracting apoplasm fluid from plant root system

Technical Field

The utility model relates to the technical field of biology, in particular to a device for extracting apoplast body fluid from a plant root system.

Background

Apoplast, refers to a system consisting of cell walls, intercellular spaces and ducts at the periphery of plant cell protoplasts. The apoplast is a channel for transporting water and solute in the plant body, is commonly present in organs such as roots, stems, leaves and the like of the plant, and is continuous in the whole plant. Apoplast is a living space with important physiological functions that can store and transform substances, accumulate and utilize nutrients, interact and signal conduction between plants and microorganisms, and make adaptive response to environmental stress. After the root system of the plant is infected by external pathogens, the root system of the plant can secrete some proteins into apoplast body fluid, pathogenic bacteria can secrete some proteins into apoplast body fluid, the proteins are the most direct acting proteins, and the analysis of the action and interaction mechanism of the proteins is particularly important for researching the pathogenic mechanism of the pathogenic bacteria, so that clean and pollution-free apoplast body fluid is extracted for mass spectrometry. The existing extraction device needs further separation after solid-liquid mixing and centrifugation after extraction, is troublesome to use and cannot obtain clean and pollution-free extraplasmic body fluid.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a device for extracting apoplast body fluid from a plant root system, which is provided with a separation net, wherein the root system is placed on the separation net, so that the trouble that solid and liquid need to be separated after centrifugation is avoided, clean and pollution-free apoplast body fluid is obtained, and the problems in the background art are solved.

In order to achieve the purpose, the utility model provides the following technical scheme:

a device for extracting apoplasm body fluid from a plant root system comprises a pipe body, a collection bin, an upper cover, a separation net and an opening and closing mechanism; the collecting bin is detachably connected to the bottom end of the pipe body, and the upper cover is clamped at the top of the pipe body; the separation net is detachably connected inside the pipe body; the opening and closing mechanism comprises a claw, a knob and a ratchet wheel; the clamping jaw is hinged to the pipe body, an elastic piece is connected between the clamping jaw and the pipe body, the knob is rotatably connected to the outer wall of the pipe body, and the ratchet wheel is connected to the knob; the clamping jaws are clamped on the upper cover so that the upper cover is tightly buckled at the top end of the pipe body; when the ratchet wheel rotates, the claw is pushed to be separated from the upper cover.

According to a further improvement scheme of the utility model, an annular step groove is formed in the outer wall of the top end of the tube body, and the bottom end of the upper cover is clamped in the step groove; the upper cover is connected with the pipe body through a hinge, and an annular flange is arranged at the bottom end of the upper cover.

The utility model has the further improvement scheme that the outer wall of the top of the pipe body is connected with three support seats, the clamping jaws are hinged on the support seats, and the tops of the clamping jaws are clamped on a flange at the bottom of the upper cover.

The utility model has the further improvement scheme that the elastic piece is a spring, one end of the spring is connected to the inner side of the upper part of the clamping jaw, and the other end of the spring is connected with the outer wall of the pipe body.

The utility model has the further improvement scheme that the knob is in threaded fit with the outer wall of the pipe body, the ratchet wheel is fixedly connected to the top of the knob, and the bottom end of the claw is in contact with the ratchet wheel.

The further improvement scheme of the utility model is that the outer wall of the tube body above the knob is connected with a cover plate, and the cover plate is provided with a notch for the clamping jaw to pass through.

The utility model has the further improvement scheme that the collecting bin is in an inverted cone-shaped structure, and the collecting bin is in threaded fit with the bottom end of the pipe body.

The utility model has the further improvement scheme that a protruded fixing table is arranged in the middle of the pipe body, a fixing clamp is connected to the fixing table, the other end of the fixing clamp is clamped in a clamping groove on a separation net, and a plurality of infiltration holes are formed in the separation net.

The further improvement scheme of the utility model is that the pore size of the infiltration pores is 70-200 um.

The utility model has the beneficial effects that:

firstly, the device for extracting the extraplasmic body fluid from the plant root system is provided with the separation net, and the root system is placed on the separation net, so that the trouble that solid and liquid need to be separated after centrifugation is avoided, and clean and pollution-free extraplasmic body fluid is obtained.

Secondly, according to the device for extracting the apoplast body fluid from the plant root system, the pipe body is in threaded connection with the collecting bin, and the clean and pollution-free apoplast body fluid can be obtained only by screwing off the collecting bin after centrifugation is finished, so that the trouble of dumping the liquid after the root system is cleaned is avoided.

Thirdly, the separation net of the device for extracting the extraplasmic body fluid from the plant root system is detachable, and the separation net can be removed when not needed.

Fourthly, the device for extracting the apoplast body fluid from the plant root system is provided with the opening and closing mechanism, so that the experiment personnel can operate the device with one hand while the upper cover is covered, and the upper cover can be opened by rotating the knob, thereby facilitating the operation of the experiment personnel.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is an overall sectional view of the present invention.

Fig. 3 is a partially enlarged view of a portion a in fig. 2.

Fig. 4 is a schematic view of the collecting bin structure of the present invention.

FIG. 5 is a schematic view of the ratchet structure of the present invention.

Fig. 6 is a schematic view of the structure of the separation net of the present invention.

Fig. 7 is a partial cross-sectional view of the present invention.

In the figure: 1-pipe body, 101-support, 102-fixed table, 103-fixed clamp, 2-collection bin, 3-upper cover, 301-flange, 302-hinge, 4-separation net, 401-percolation hole, 402-clamping groove, 5-opening and closing mechanism, 501-claw, 502-knob, 503-ratchet, 504-elastic piece, 6-cover plate and 601-notch.

Detailed Description

The utility model is further elucidated with reference to the drawings and the embodiments.

Example 1: as shown in fig. 1 to 7, a device for extracting apoplast body fluid from a plant root system comprises a pipe body 1, a collection bin 2, an upper cover 3, a separation net 4 and an opening and closing mechanism 5; the collecting bin 2 is detachably connected to the bottom end of the pipe body 1, and the upper cover 3 is clamped to the top of the pipe body 1; the separation net 4 is detachably connected inside the pipe body 1; the opening and closing mechanism 5 comprises a claw 501, a knob 502 and a ratchet 503; the jaw 501 is hinged to the pipe body 1, an elastic piece 504 is connected between the jaw 501 and the pipe body 1, the knob 502 is rotatably connected to the outer wall of the pipe body 1, and the ratchet 503 is connected to the knob 502; the clamping jaws 501 are clamped on the upper cover 3, so that the upper cover 3 is tightly buckled on the top end of the pipe body 1; when the ratchet 503 rotates, the claw 501 is pushed to be separated from the upper cover 3; the outer wall of the top end of the pipe body 1 is provided with an annular step groove, and the bottom end of the upper cover 3 is clamped in the step groove; the upper cover 3 is connected with the pipe body 1 through a hinge 302, and the bottom end of the upper cover 3 is provided with an annular flange 301; the outer wall of the top of the pipe body 1 is connected with three support seats 101, the clamping jaws 501 are hinged on the support seats 101, and the tops of the clamping jaws 501 are clamped on the flanges 301 at the bottoms of the upper covers 3; the elastic piece 504 is a spring, one end of the spring is connected to the inner side of the upper part of the clamping jaw 501, and the other end of the spring is connected with the outer wall of the pipe body 1; the knob 502 is in threaded fit with the outer wall of the pipe body 1, the ratchet 503 is fixedly connected to the top of the knob 502, and the bottom end of the claw 501 is in contact with the ratchet 503; the outer wall of the pipe body 1 above the knob 502 is connected with a cover plate 6, and the cover plate 6 is provided with a notch 601 for the claw 501 to penetrate through; the collecting bin 2 is of an inverted cone-shaped structure, and the collecting bin 2 is in threaded fit with the bottom end of the pipe body 1; a protruded fixing table 102 is arranged in the middle of the pipe body 1, a fixing clip 103 is connected to the fixing table 102, the other end of the fixing clip 103 is clipped in a clipping groove 402 on the separation net 4, and a plurality of percolation holes 401 are arranged on the separation net 4; the pore size of the infiltration holes 401 is 70-200 um; in this embodiment, the pore size of the percolation well 401 is 70 um.

Wherein, body 1, collection storehouse 2, upper cover 3 and opening and shutting mechanism 5 are the injection molding, and separation net 4 comprises frame and dictyosome, and the dictyosome is the nylon material, and draw-in groove 402 sets up on the frame.

The specific working principle of the utility model is as follows: when extraplasmic body fluid needs to be extracted, an experimenter pushes the separation net 4 into the pipe body 1, and the separation net 4 moves downwards to enable the fixing clamp 103 to penetrate into the clamping groove 402 to be clamped with the separation net 4; when the roots need to be placed, an experimenter holds the pipe body 1 with one hand, pushes the knob 502 to enable the knob 502 to rotate, the knob 502 drives the ratchet wheel 503 to rotate, the ratchet wheel 503 loosens the lower part of the jaw 501, the jaw 501 pushes the upper part of the jaw 501 under the action of the spring, the upper cover 3 is loosened by the upper part of the jaw 501, at the moment, the upper cover 3 can be opened, then the roots are placed on the separation net 4, the upper cover 3 is covered, the knob 502 is rotated reversely, the knob 502 drives the ratchet wheel 503 to rotate, the ratchet wheel 503 pushes the lower part of the jaw 501, and the jaw 501 compresses the spring and simultaneously clamps the upper cover 3; the device is put into a centrifuge for centrifugation, and the collection bin 2 is screwed off after centrifugation, so that clean and pollution-free extraplasmic body fluid can be obtained.

The above embodiments are merely illustrative of the technical concepts and features of the present invention, and the purpose of the embodiments is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims

1. A device for extracting apoplast body fluid from a plant root system is characterized in that: comprises a pipe body (1), a collection bin (2), an upper cover (3), a separation net (4) and an opening and closing mechanism (5); the collecting bin (2) is detachably connected to the bottom end of the pipe body (1), and the upper cover (3) is clamped to the top of the pipe body (1); the separation net (4) is detachably connected to the inside of the pipe body (1); the opening and closing mechanism (5) comprises a claw (501), a knob (502) and a ratchet wheel (503); the clamping jaw (501) is hinged to the pipe body (1), an elastic piece (504) is connected between the clamping jaw (501) and the pipe body (1), the knob (502) is rotatably connected to the outer wall of the pipe body (1), and the ratchet wheel (503) is connected to the knob (502); the clamping jaw (501) is clamped on the upper cover (3) so that the upper cover (3) is tightly buckled on the top end of the pipe body (1); when the ratchet wheel (503) rotates, the claw (501) is pushed to be separated from the upper cover (3).

2. A device for extracting extra-corporeal fluid from a plant root system as claimed in claim 1, in which: the outer wall of the top end of the pipe body (1) is provided with an annular step groove, and the bottom end of the upper cover (3) is clamped in the step groove; the upper cover (3) is connected with the pipe body (1) through a hinge (302), and an annular flange (301) is arranged at the bottom end of the upper cover (3).

3. A device for extracting extra-corporeal fluid from a plant root system as claimed in claim 2, in which: body (1) top outer wall is connected with three support (101), and jack catch (501) articulate on support (101), and jack catch (501) top joint is on flange (301) of upper cover (3) bottom.

4. A device for extracting extra-corporeal fluid from a plant root system as claimed in claim 3, in which: the elastic piece (504) is a spring, one end of the spring is connected to the inner side of the upper part of the clamping jaw (501), and the other end of the spring is connected with the outer wall of the pipe body (1).

5. A device for extracting extra-corporeal fluid from a plant root system as claimed in claim 3, in which: the knob (502) is in threaded fit with the outer wall of the pipe body (1), the ratchet wheel (503) is fixedly connected to the top of the knob (502), and the bottom end of the clamping jaw (501) is in contact with the ratchet wheel (503).

6. A device for extracting extra-corporeal fluid from a plant root system as claimed in claim 3, in which: the outer wall of the pipe body (1) above the knob (502) is connected with a cover plate (6), and the cover plate (6) is provided with an opening (601) for the clamping jaw (501) to penetrate through.

7. A device for extracting extra-corporeal fluid from a plant root system as claimed in claim 1, in which: collect storehouse (2) and be back taper structure, and collect storehouse (2) screw-thread fit in the bottom of body (1).

8. A device for extracting extra-corporeal fluid from a plant root system as claimed in claim 1, in which: the pipe body (1) middle part is provided with convex fixed station (102), is connected with fixing clip (103) on fixed station (102), and the other end joint of fixing clip (103) is equipped with a plurality of infiltration hole (401) in draw-in groove (402) on separation net (4).

9. An apparatus for extracting apoplasmic body fluid from a plant root system as claimed in claim 8, wherein: the pore size of the infiltration holes (401) is 70-200 um.