CN203112846U - Screw multi-level adjustable cell culture flask - Google Patents

Screw multi-level adjustable cell culture flask Download PDF

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Publication number
CN203112846U
CN203112846U CN 201320039014 CN201320039014U CN203112846U CN 203112846 U CN203112846 U CN 203112846U CN 201320039014 CN201320039014 CN 201320039014 CN 201320039014 U CN201320039014 U CN 201320039014U CN 203112846 U CN203112846 U CN 203112846U
Authority
CN
China
Prior art keywords
groove
projection
bottle cap
screw
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 201320039014
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Chinese (zh)
Inventor
梁盈
林亲录
钟海雁
吴伟
方婧杰
高宇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Central South University of Forestry and Technology
Original Assignee
Central South University of Forestry and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Central South University of Forestry and Technology filed Critical Central South University of Forestry and Technology
Priority to CN 201320039014 priority Critical patent/CN203112846U/en
Application granted granted Critical
Publication of CN203112846U publication Critical patent/CN203112846U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model discloses a screw multi-level adjustable cell culture flask which comprises a lid and a body with a threaded mouth, wherein threads of the threaded mouth are provided with grooves or protrusions, and the threads of the lid are provided with protrusions matched with the grooves or provided with grooves matched with the protrusions. When the protrusions and the grooves come into contact with each other, sound can be generated, to position and control cell air penetrability. The cell culture flask has the advantages that the cell air penetrability can be favorably operated and uniformly controlled and cell growth state can be stabilized.

Description

Many grades of adjustable Tissue Culture Flasks of a kind of screw socket
Technical field
The utility model relates to the cytobiology field, relates in particular to a kind of CO of being applicable to 2The Tissue Culture Flask of incubator.
Background technology
When carrying out cell cultures, zooblast needs constantly to supply with oxygen and get rid of carbonic acid gas, vegetable cell then needs constantly to supply with carbonic acid gas and gets rid of oxygen, thereby Tissue Culture Flask is extremely important to the discharge of the supply of oxygen or carbonic acid gas and carbonic acid gas or oxygen.In existing Tissue Culture Flask, mainly comprise body, bottleneck and bottle cap, but the following problem of ubiquity:
1. most Tissue Culture Flask does not have ventilating structure, it mainly is the circulation of controlling oxygen and carbonic acid gas by the elasticity of control bottle cap, these gas flow are to enter from all slits that bottle cap and bottleneck join, such mode can be supplied with the gas flow of culturing bottle, but air penetrability is difficult to hold, the living environment instability of cell directly causes the instability of cell growth state.
2. existing Tissue Culture Flask is when carrying out cell cultures, be difficult to get hold of the elasticity of bottle cap, during open culture generally unscrewing half-turn degree of being after screwing again, but air permeable effect instability often, medium pH value and osmotic pressure in the cell bottle can't be kept perfect condition; And during airtight cultivation, again often screw socket bottle cap overtorquing, cause the screw socket stripped thread, destroy the stopping property of culturing bottle.
3. there is the bottle cap of some band breather holes on the market, also has the filter membrane of 0.2 m, prevent that culturing cell is contaminated, the invisible rising of scientific research cost.
The utility model content
At the deficiencies in the prior art part, the utility model aims to provide many grades of adjustable Tissue Culture Flasks of screw socket of a kind of even control cell air penetrability.This Tissue Culture Flask can arrange the air penetrability of cell as required, and easy to operate, cell air penetrability is controlled evenly, the growth conditions of stabilized cell.
For achieving the above object, the technical solution of the utility model is:
Many grades of adjustable Tissue Culture Flasks of a kind of screw socket, comprise bottle cap and the bottle that is threaded bottleneck, the screw thread of described screw thread bottleneck is provided with groove or projection, and the screw thread of bottle cap is provided with the projection that is complementary with this groove or the groove that is complementary with this projection.
Be preferably: the outside screw of described screw thread bottleneck is provided with a plurality of grooves or projection, and the internal thread of bottle cap is provided with projection or a groove that is complementary with this projection that is complementary with this groove; Perhaps the internal thread of described screw thread bottleneck is provided with a plurality of grooves or projection, and the outside screw of bottle cap is provided with projection or a groove that is complementary with this projection that is complementary with this groove.
More preferably: the outside screw of described screw thread bottleneck is provided with three grooves, and the internal thread of bottle cap is provided with a projection that is complementary with this groove; First groove a is arranged on the outside screw of first lap, and second groove b is arranged on the outside screw of circle second from the bottom, and the 3rd groove c is arranged on the outside screw terminal point; Described projection is arranged on the zero position of the internal thread of bottle cap.
Also be preferably: the internal thread of described screw thread bottleneck is provided with three projectioies, and the outside screw of bottle cap is provided with a groove that is complementary with this projection; First protruding a is arranged on the internal thread of first lap, and second protruding b is arranged on the internal thread of circle second from the bottom, and the 3rd protruding c is arranged on the internal thread terminal point; Described groove is arranged on the externally threaded zero position of bottle cap.
More preferably: second groove b or second protruding b are arranged on from circle round 180 ° position of screw thread terminal point.
The thickness of described projection is preferred≤1mm.
Below the utility model is made further explanation
Tissue Culture Flask described in the utility model comprises body and bottle cap, and body is provided with bottleneck, and the bottleneck upper end is bottleneck; The screw thread of described screw thread bottleneck is provided with groove or projection, and the screw thread of bottle cap is provided with the projection that is complementary with this groove or the groove that is complementary with this projection; When projection and contact grooves, can send the sound of " clatter ".The cell air penetrability is 50-70% when preferably arranging first grade; The cell air penetrability is 20-40% in the time of second grade; Third gear is for just screwing, and is airtight.
The adjustable design of third gear described in the utility model is applicable to 25cm 2-225cm 2In the culturing bottle of arbitrary specification, no matter torticollis, straight neck.
Compared with prior art, advantage of the present utility model is:
1, thereby Tissue Culture Flask of the present utility model can be controlled the elasticity control oxygen of bottle cap and the circulation of carbonic acid gas by many grades of adjustings, regulates the air penetrability of cell cultures as required, and the control of cell air penetrability is even, the growth conditions of stabilized cell.
2, Tissue Culture Flask of the present utility model can " clatter " sound by last step judges that the screw socket bottle cap tightens, and can not cause the screw socket stripped thread, guarantees the stopping property of culturing bottle.
3, Tissue Culture Flask of the present utility model both can be used as open culture, can be used as airtight cultivation again, and cost is far below the ventilative culturing bottle of import.
Description of drawings
Fig. 1 is the Bottle cap structure synoptic diagram of the many grades of adjustable Tissue Culture Flasks of a kind of screw socket of embodiment;
Fig. 2 is the thread bottle mouth structure synoptic diagram of the many grades of adjustable Tissue Culture Flasks of a kind of screw socket of embodiment, and wherein second protruding b is dotted line, is illustrated in the back side of this front view, the screw thread terminal point circle round 180 the degree positions;
In the drawings, the 1st, bottle cap, the 2nd, the screw thread bottleneck, the 3rd, first protruding a, the 4th, groove, 5 is second protruding b, 6 is the 3rd protruding c.
Embodiment
Below in conjunction with embodiment and accompanying drawing the utility model is described further.
As depicted in figs. 1 and 2, many grades of adjustable Tissue Culture Flasks of a kind of screw socket, comprise bottle cap 1 and the bottle that is threaded bottleneck 2, the screw thread of described bottle cap 1 is provided with groove 4 or projection, and the screw thread of bottleneck is provided with the projection that is complementary with this groove 4 or the groove 4 that is complementary with this projection.
Used bottle and bottle cap material are with plasma treated macromolecular material, as polystyrene.The projection that three about 0.8mm of thickness are arranged at the bottleneck internal thread, first protruding a (screws in the position of 360 degree) on the screw thread first lap initial by bottleneck, the half-turn that second protruding b circles round at the screw thread terminating point positions of 180 degree (the screw thread terminal point circle round), the 3rd protruding c is on the internal thread terminating point.And have only a groove 4 that is complementary with above-mentioned projection on the corresponding bottle cap outside screw, be arranged on the bottle cap outside screw starting point.
When bottle cap begins to screw in, be screwed into 360 when spending, can run into first protruding a, be first gear, first protruding a falls into groove 4, sends the sound of " clatter ", and the cell air penetrability is about 50-70% when being positioned at herein; Bottle cap continues to screw in, and runs into second protruding b, is second gear, and second protruding b falls into groove 4, sends the sound of " clatter ", and the cell air penetrability is about 20-40% when being positioned at herein; Bottle cap continues to screw in 180 degree, runs into the 3rd protruding c, is third gear, and the 3rd protruding c falls into groove 4, sends the sound of " clatter ", and when being positioned at herein, bottle cap is tightened, stopping property the best.
Above-described embodiment only is in order to set forth a kind of embodiment that principle of work of the present utility model provides, to be not restricted to the data of mark in embodiment and the accompanying drawing.Every based on the utility model, its technical characterictic is simply replaced or reconfigured, and do not break away from the technical scheme of the utility model flesh and blood, all should belong within the scope that the utility model protects.

Claims (6)

1. many grades of adjustable Tissue Culture Flasks of a screw socket, comprise bottle cap and the bottle that is threaded bottleneck, it is characterized in that the screw thread of described screw thread bottleneck is provided with groove or projection, and the screw thread of bottle cap is provided with the projection that is complementary with this groove or the groove that is complementary with this projection.
2. according to many grades of adjustable Tissue Culture Flasks of the described screw socket of claim 1, it is characterized in that, the outside screw of described screw thread bottleneck is provided with a plurality of grooves or projection, and the internal thread of bottle cap is provided with projection or a groove that is complementary with this projection that is complementary with this groove; Perhaps the internal thread of described screw thread bottleneck is provided with a plurality of grooves or projection, and the outside screw of bottle cap is provided with projection or a groove that is complementary with this projection that is complementary with this groove.
3. according to many grades of adjustable Tissue Culture Flasks of the described screw socket of claim 2, it is characterized in that the outside screw of described screw thread bottleneck is provided with three grooves, and the internal thread of bottle cap is provided with a projection that is complementary with this groove; First groove a is arranged on the outside screw of first lap, and second groove b is arranged on the outside screw of circle second from the bottom, and the 3rd groove c is arranged on the outside screw terminal point; Described projection is arranged on the zero position of the internal thread of bottle cap.
4. according to many grades of adjustable Tissue Culture Flasks of the described screw socket of claim 2, it is characterized in that the internal thread of described screw thread bottleneck is provided with three projectioies, and the outside screw of bottle cap is provided with a groove that is complementary with this projection; First protruding a is arranged on the internal thread of first lap, and second protruding b is arranged on the internal thread of circle second from the bottom, and the 3rd protruding c is arranged on the internal thread terminal point; Described groove is arranged on the externally threaded zero position of bottle cap.
5. according to claim 3 or many grades of adjustable Tissue Culture Flasks of 4 described screw sockets, it is characterized in that second groove b or second protruding b are arranged on from circle round 180 ° position of screw thread terminal point.
6. according to many grades of adjustable Tissue Culture Flasks of the described screw socket of one of claim 1-4, it is characterized in that the thickness≤1mm of described projection.
CN 201320039014 2013-01-24 2013-01-24 Screw multi-level adjustable cell culture flask Expired - Fee Related CN203112846U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201320039014 CN203112846U (en) 2013-01-24 2013-01-24 Screw multi-level adjustable cell culture flask

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201320039014 CN203112846U (en) 2013-01-24 2013-01-24 Screw multi-level adjustable cell culture flask

Publications (1)

Publication Number Publication Date
CN203112846U true CN203112846U (en) 2013-08-07

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201320039014 Expired - Fee Related CN203112846U (en) 2013-01-24 2013-01-24 Screw multi-level adjustable cell culture flask

Country Status (1)

Country Link
CN (1) CN203112846U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104722341A (en) * 2013-11-26 2015-06-24 埃佩多夫股份公司 Culture vessel with screw-on lid

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104722341A (en) * 2013-11-26 2015-06-24 埃佩多夫股份公司 Culture vessel with screw-on lid
US9776771B2 (en) 2013-11-26 2017-10-03 Eppendorf Ag Screw cap lidded container
CN104722341B (en) * 2013-11-26 2018-08-21 埃佩多夫股份公司 Screw-cap lidded container

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Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20130807

Termination date: 20150124

EXPY Termination of patent right or utility model