CN203087160U - Conical air-permeable test tube - Google Patents

Conical air-permeable test tube Download PDF

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Publication number
CN203087160U
CN203087160U CN 201320062994 CN201320062994U CN203087160U CN 203087160 U CN203087160 U CN 203087160U CN 201320062994 CN201320062994 CN 201320062994 CN 201320062994 U CN201320062994 U CN 201320062994U CN 203087160 U CN203087160 U CN 203087160U
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CN
China
Prior art keywords
test tube
air
taper
diameter
screw lid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 201320062994
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Chinese (zh)
Inventor
华玉伟
黄天带
黄华孙
孙爱花
戴雪梅
周权男
方家林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rubber Research Institute Chinese Academy Tropical Agricultural Sciences
Original Assignee
Rubber Research Institute Chinese Academy Tropical Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rubber Research Institute Chinese Academy Tropical Agricultural Sciences filed Critical Rubber Research Institute Chinese Academy Tropical Agricultural Sciences
Priority to CN 201320062994 priority Critical patent/CN203087160U/en
Application granted granted Critical
Publication of CN203087160U publication Critical patent/CN203087160U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model relates to a conical air-permeable test tube, consisting of a spiral cover, an air filtering device, a sealing washer and a conical test tube, wherein an air-permeable groove and an air-permeable hole are formed in the center of the top of the spiral cover; an air filtering membrane and a membrane pressing sheet of the air filtering device are placed in the air-permeable groove; the sealing washer is placed in the spiral cover; and the spiral cover is spirally sleeved on a spiral mouth of the conical test tube. By using the sealing washer and the air filtering device, the conical air-permeable test tube effectively solves the problems that plant growth is influenced and the air-permeable pollution rate is high because of a closed process of cultivating somatic embryos to regenerate plants. The conical air-permeable test tube effectively solves the problems of waste of a culture medium and insufficient in-tube length required by root growth in the process of cultivating somatic embryos to regenerate plants, thus saving the culture medium and reducing the production cost.

Description

A kind of taper test tube of breathing freely
Technical field
The utility model relates to the ventilative test tube of a kind of taper, belongs to field of plant cell engineering technology.
Background technology
Bamboo grows body embryo plant is the plant that directly regenerates and form by somatic embryo, has juvenile form clone from root system, field experiment confirms that its output and growth all are better than producing at present the bud grafting stereotype clone planting material of going up main cultivation, have become the main planting material of natural rubber industry development from now on and application.At present; China has taken the lead in implementing the key technology of bamboo grows body embryo plant scale breeding, finds fungi and germ contamination especially severe in the batch production production process; except culturing room's environment caused, it was of paramount importance reason that body embryo plant regeneration device is not suitable for the cultivation of bamboo grows body embryo plant.
The culture apparatus of existing bamboo grows body embryo plant is a screw-cap test tube, mainly realize managing the isolation of internal and external environment by screwing of lid, its major defect is 1. screw socket seal degree and pollution level positive correlation, and it is tight more that the lid of screw-cap test tube revolves, and seal degree is good more, pollution rate is low more, but to be body embryo plant be cut off oxygen deficiency in the test tube to the demand of oxygen to the problem of thereupon bringing, cause body embryo plant strain growth to be suppressed, even stop growing and death; 2. screw-cap test tube is cylindrical, and medium root system at least can't stretch and crooked, and medium is wasted medium at most, increases production cost; Therefore, need to improve the culture apparatus of bamboo grows body embryo plant.
Summary of the invention
The purpose of this utility model provides a kind of ventilative test tube of taper that is used for bamboo grows body embryo plant regeneration, to solve the problem that bamboo grows body embryo plant incubation is easily polluted and cost is cultivated in reduction.
In order to realize the purpose of this utility model, the ventilative test tube of a kind of taper of the utility model is made up of screw lid, air filter, seal washer and taper test tube.There are ventilating groove and air-vent in screw lid caping center, and ventilating groove is settled the air filtering film and the pressuring film plate of air filter; Seal washer is placed in the screw lid lid, and the screw lid rotation set is in the taper test tube.
Described screw lid external diameter 29~31mm, internal diameter 28.4~28.6mm; The screw lid caping has ventilating groove and air-vent, and ventilating groove is in screw lid top center position, and diameter is 15~17mm, groove depth 1.4~1.6mm, ventilating groove cell wall have three bayonet slot that are evenly distributed, and air-vent is at the ventilating groove core, diameter 2~3mm, air-vent is as ventilation hole; In the screw lid lid internal thread is arranged, be complementary with the external screw thread of taper test tube spiral mouth, but the screw lid rotation set is in the spiral mouth.Screw lid with high temperature resistant PEP as manufacturing materials.
Described air filter comprises air filtering film and pressuring film plate.Air filtering film diameter 15~17mm, thickness 0.11~0.13mm, aperture 0.21~0.23 μ m; Pressuring film plate diameter 15~17mm, thickness 1.2~1.4mm has equally distributed 6 scallop holes, as ventilation hole on the pressuring film plate; There are three bayonet sockets that are evenly distributed at the pressuring film plate edge, and is used with the bayonet slot of ventilating groove, blocks pressuring film plate.The air filtering film is the carbonic acid tunica fibrosa, and pressuring film plate selects for use PC as manufacturing materials.
Described seal washer internal diameter 23~25mm, external diameter 26~28mm, thickness 1.4~1.6mm; Seal washer is placed in the screw lid lid.Seal washer uses silica gel as manufacturing materials.
High 249~the 251mm of described taper test tube, diameter 29~31mm, wall thickness 0.9~1.0mm; The upper port of taper test tube is for there being externally threaded spiral mouth, and the internal thread of external screw thread and screw lid is complementary, and allows the screw lid rotation set in the spiral mouth, spiral mouth external diameter 28.3~28.5mm, internal diameter 25~27mm; The bottom of taper test tube is a conical pipe, the high 99~101mm of conical pipe.The taper test tube with PC or glass as manufacturing materials.
A kind of taper of the utility model test tube of breathing freely is used to cultivate bamboo grows somatic embryo regeneration plant, by the use of seal washer and air filter, efficiently solves airtight plant strain growth, the ventilative high problem of pollution rate of influencing of bamboo grows body embryo plant incubation.Simultaneously, the ventilative test tube of taper is guaranteeing to cultivate under the enough situations of base unit weight, prolonged the space that bamboo grows somatic embryo regeneration plant root stretches, efficiently solve the bamboo grows of test tube cultivation in the past somatic embryo regeneration plant and have the not enough problem of length in waste medium and the required pipe of bamboo grows somatic embryo regeneration plant root growth, save medium, reduce production costs.The ventilative test tube of taper also can be used for the cultivation of other plant or animal tissue or cell, has extensive use.
Description of drawings
Fig. 1: the taper test tube figure that breathes freely
Among the figure: 1. screw lid; 2. taper test tube.
Fig. 2: screw lid figure
Among the figure: 1. screw lid; 3. ventilating groove; 4. air-vent; 5. bayonet slot.
Fig. 3: air filter figure
Among the figure: 6. pressuring film plate; 7. scallop hole; 8. bayonet socket; 9. air filtering film.
Fig. 4: sealing gasket loop graph
Among the figure: 10. seal washer.
Fig. 5: taper test tube figure
Among the figure: 11. external screw threads; 12. conical pipe.
Embodiment
Following examples are used to illustrate the utility model, but are not used for limiting scope of the present utility model.
A kind of taper of the utility model test tube of breathing freely, as Fig. 1 to shown in Figure 5: comprise screw lid (1), taper test tube (2), ventilating groove (3), air-vent (4), bayonet slot (5), pressuring film plate (6), scallop hole (7), bayonet socket (8), air filtering film (9), seal washer (10), external screw thread (11), conical pipe (12).During making, screw lid external diameter 30mm, internal diameter 28.5mm; Ventilating groove is in screw lid top center position, and diameter 16mm, groove depth 1.5mm, cell wall have three bayonet slot that are evenly distributed; Air-vent is at the ventilating groove core, diameter 2.5mm.Air filtering film diameter 16mm, thickness 0.12mm, aperture 0.22 μ m; Pressuring film plate diameter 16mm, thickness 1.3mm has equally distributed 6 scallop holes as ventilation hole on the pressuring film plate, and there are three bayonet sockets that are evenly distributed at the pressuring film plate edge, and uses with the bayonet slot coupling.Seal washer internal diameter 24mm, external diameter 27mm, thickness 1.5mm.The high 250mm of taper test tube, diameter 30mm, wall thickness 1.2mm; Taper test tube upper port is the spiral mouth, spiral mouth external diameter 28.4mm, and internal diameter 26mm, taper test tube bottom is a conical pipe, the high 100mm of conical pipe, wall thickness 1.0mm; The mouth of pipe of taper test tube is the spiral mouth, and external screw thread is arranged, and the external screw thread of spiral mouth and the internal thread of screw lid match, and spiral mouth and screw lid are complementary, but the screw lid rotation set is in the spiral mouth.
The air filtering film selects for use the carbonic acid fiber as manufacturing materials, and screw lid selects for use PEP as manufacturing materials, and seal washer selects for use silica gel as manufacturing materials, and the taper test tube selects for use PC or glass as manufacturing materials, and pressuring film plate selects for use PC as manufacturing materials.
During use, earlier the air filtering film is installed in the ventilating groove of screw lid, cover air-vent, again pressuring film plate is loaded on the top of air filtering film, utilize the bayonet slot of ventilating groove and the bayonet socket of pressuring film plate to screw pressuring film plate, realize the fixing of air filtering film; Then, with the seal washer pad in screw lid, at last with the screw lid rotation set on the taper test tube that installs medium and culture materials, screw; The taper test tube is ventilative by the air-vent and the air filtering film of screw lid, realizes taper breathing freely with aseptic of culture environment in vitro.When adding medium, medium is as long as fill full centrum in vitro partly.
Though above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Claims (5)

1. the ventilative test tube of a taper is characterized in that the ventilative test tube of taper is made up of screw lid, air filter, seal washer and taper test tube; There are ventilating groove and air-vent in screw lid caping center, and ventilating groove is settled the air filtering film and the pressuring film plate of air filter; Seal washer is placed in the screw lid lid, and the screw lid rotation set is in the taper test tube.
2. a kind of taper according to claim 1 test tube of breathing freely is characterized in that air filtering membrane aperture 0.21~0.23 μ m, diameter 15~17mm, thickness 0.11~0.13mm; Pressuring film plate diameter 15~17mm, thickness 1.2~1.4mm has equally distributed 6 scallop holes on the pressuring film plate.
3. a kind of taper according to claim 1 test tube of breathing freely is characterized in that screw lid external diameter 29~31mm, internal diameter 28.4~28.6mm; Ventilating groove is in screw lid top center position, and diameter 15~17mm, groove depth 1.4~1.6mm, ventilating groove cell wall have three bayonet slot that are evenly distributed; Air-vent is at the ventilating groove core, diameter 2~3mm; Screw lid with high temperature resistant PEP as manufacturing materials.
4. a kind of taper according to claim 1 test tube of breathing freely is characterized in that seal washer internal diameter 23~25mm, external diameter 26~28mm, thickness 1.4~1.6mm; Seal washer uses silica gel as manufacturing materials.
5. a kind of taper according to claim 1 test tube of breathing freely is characterized in that the high 249~251mm of taper test tube, diameter 29~31mm, wall thickness 0.9~1.0mm, the high 99~101mm of tapering part.
CN 201320062994 2013-02-04 2013-02-04 Conical air-permeable test tube Expired - Fee Related CN203087160U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201320062994 CN203087160U (en) 2013-02-04 2013-02-04 Conical air-permeable test tube

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201320062994 CN203087160U (en) 2013-02-04 2013-02-04 Conical air-permeable test tube

Publications (1)

Publication Number Publication Date
CN203087160U true CN203087160U (en) 2013-07-31

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Application Number Title Priority Date Filing Date
CN 201320062994 Expired - Fee Related CN203087160U (en) 2013-02-04 2013-02-04 Conical air-permeable test tube

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CN (1) CN203087160U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105104359A (en) * 2015-08-23 2015-12-02 罗丽秀 Test tube suitable for storing and carrying insect living bodies outdoors

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105104359A (en) * 2015-08-23 2015-12-02 罗丽秀 Test tube suitable for storing and carrying insect living bodies outdoors

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20130731

Termination date: 20150204

EXPY Termination of patent right or utility model