CN204232156U - Be beneficial to the cell transportation device maintaining cytoactive - Google Patents
Be beneficial to the cell transportation device maintaining cytoactive Download PDFInfo
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- CN204232156U CN204232156U CN201420671357.8U CN201420671357U CN204232156U CN 204232156 U CN204232156 U CN 204232156U CN 201420671357 U CN201420671357 U CN 201420671357U CN 204232156 U CN204232156 U CN 204232156U
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- Prior art keywords
- cell
- beneficial
- cytoactive
- transportation device
- temperature receiver
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Abstract
The utility model discloses a kind of cell transportation device being beneficial to maintenance cytoactive, comprise casing and cover the lid on described casing, being arranged with storage storage tank in described casing top, in described storage storage tank, being removably connected with cell culture apparatus; Described bottom half and sidepiece are communicated with the cavity be provided with for holding low-temperature receiver, and described casing top is provided with at least one low-temperature receiver inlet being communicated with described cavity, and described low-temperature receiver inlet is sealed with low-temperature receiver seal cover; Described cell culture apparatus comprises and has cavity volume for holding the housing of cell suspension, and described housing upper is respectively equipped with cell inoculation mouth, CO
2aeration filtering device and CO
2concentrations detectors.The utility model is by described CO
2aeration filtering device effectively can block CO
2in contained bacterial micro-organism, guarantee to keep germ-free condition in cell culture apparatus, be beneficial in cell long-distance transport process and maintain cytoactive, cell traffic process is impaired little, is beneficial to strange land and carries out Secondary Culture.
Description
Technical field
The utility model relates to cell technical field of transportation, is specifically related to a kind of cell transportation device being beneficial to maintenance cytoactive.
Background technology
Along with advancing by leaps and bounds of biotechnology, the application of cell is more and more extensive, and long-range cell transport is also can hardly be avoided.Traditional cell means of transportation first cell is cultured to adherent growth in blake bottle, be more directly placed in ice chest by blake bottle and carry out long-distance transport.In this case, owing to can not control the gas composition in blake bottle, cytoactive is badly damaged.After arriving destination, cell often shows the phenomenons such as apoptosis increase, poor growth.Therefore, be necessary to design a kind of conveying arrangement being conducive to maintaining cytoactive, solve Problems existing in this cell long-distance transport process.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of reasonable in design, can reduce the cytoactive extent of damage in transportation, is beneficial to the cell transportation device maintaining cytoactive.
For solving the problems of the technologies described above, the technical solution of the utility model is: be beneficial to the cell transportation device maintaining cytoactive, comprise casing and cover the lid on described casing, being arranged with storage storage tank in described casing top, in described storage storage tank, being removably connected with cell culture apparatus; Described bottom half and sidepiece are communicated with the cavity be provided with for holding low-temperature receiver, and described casing top is provided with at least one low-temperature receiver inlet being communicated with described cavity, and described low-temperature receiver inlet is sealed with low-temperature receiver seal cover; Described cell culture apparatus comprises and has cavity volume for holding the housing of cell suspension, and described housing upper is respectively equipped with cell inoculation mouth, CO
2aeration filtering device and CO
2concentrations detectors.
As the improvement to technique scheme, described CO
2aeration filtering device comprises CO
2gas tube, described CO
2gas tube one end is stretched in the cavity volume of described housing, and the other end is provided with CO
2ventilating control valve, described CO
2gas tube is positioned at described CO
2the inner side of ventilating control valve is provided with CO
2filter.
As the improvement to technique scheme, the outer wall of described casing is coated is provided with insulating.
As the further improvement to technique scheme, described insulating is alumina silicate heat-insulation layer.
As the further improvement to technique scheme, the cross sectional shape of described casing is rectangle or square, and described casing and described lid are by the hinged setting of jointed shaft.
As the further improvement to technique scheme, the cross sectional shape of described casing is circular or oval, and described casing is connected by thread seal with described lid.
The utility model, by adopting technique scheme, has following beneficial effect: by described CO
2aeration filtering device effectively can block CO
2in contained bacterial micro-organism, guarantee to keep germ-free condition in cell culture apparatus, be beneficial in cell long-distance transport process and maintain cytoactive, cell traffic process is impaired little, is beneficial to strange land and carries out Secondary Culture.
Accompanying drawing explanation
The following drawings is only intended to schematically illustrate the utility model and explain, does not limit scope of the present utility model.Wherein:
Fig. 1 is the structural representation of the utility model embodiment lid when opening;
Schematic top plan view when Fig. 2 is the utility model embodiment removing lid;
Fig. 3 is the view of the utility model embodiment lid when closing;
Fig. 4 is the structural representation of cell culture apparatus.
In figure: 1-casing; 11-cavity; 12-low-temperature receiver inlet; 13-low-temperature receiver seal cover; 2-lid; 3-stores storage tank; 4-cell culture apparatus; 41-cavity volume; 42-housing; 43-cell inoculation mouth; 44-CO
2aeration filtering device; 441-CO
2gas tube; 442-CO
2ventilating control valve; 443-CO
2filter; 45-CO
2concentrations detectors; 46-inoculates seal cover; 5-jointed shaft; 6-ice cube.
Embodiment
Below in conjunction with drawings and Examples, set forth the utility model further.In the following detailed description, the mode only by illustrating describes some one exemplary embodiment of the present utility model.Undoubtedly, those of ordinary skill in the art can recognize, when not departing from spirit and scope of the present utility model, can revise by various different mode to described embodiment.Therefore, accompanying drawing is illustrative with being described in essence, instead of for limiting the protection domain of claim.
As shown in Figures 1 to 4, be beneficial to the cell transportation device maintaining cytoactive, comprise casing 1 and cover the lid 2 on described casing 1, be arranged with in described casing 1 top and store storage tank 3, in described storage storage tank 3, be removably connected with cell culture apparatus 4; Bottom described casing 1 and sidepiece be communicated with the cavity 11 be provided with for holding low-temperature receiver, described casing 1 top is provided with two and is communicated with the low-temperature receiver inlet 12 of described cavitys 11, and described low-temperature receiver inlet 12 is sealed with low-temperature receiver seal cover 13; Described cell culture apparatus 4 comprises and has cavity volume 41 for holding the housing 42 of cell suspension, and described housing 42 top is respectively equipped with cell inoculation mouth 43, CO
2aeration filtering device 44 and CO
2concentrations detectors 45, cell inoculation mouth 43 is sealed with inoculation seal cover 46.Wherein, described CO
2aeration filtering device 44 comprises CO
2gas tube 441, described CO
2gas tube 441 one end is stretched in the cavity volume 41 of described housing 42, and the other end is provided with CO
2ventilating control valve 442, described CO
2gas tube 441 is positioned at described CO
2the inner side of ventilating control valve 442 is provided with CO
2filter 443, CO
2be provided with the biological filtering membrane of diameter 0.22 μm in filter 443, effectively can block CO
2in contained bacterial micro-organism.
In order to improve the heat insulation effect of casing 1, can arrange insulating the outer wall of described casing 1 is coated, described insulating can adopt the inorganic material that the thermal conductivity factors such as alumina silicate warming plate are low.
In the present embodiment, the cross sectional shape of described casing 1 is rectangle, and lid 2, by the hinged setting of jointed shaft 5, in transportation, can be overturn sealing and implement casing 1 by described casing 1 and described lid 2.Certainly, the cross sectional shape of described casing can also adopt circle or ellipse, and when adopting circular or oval, described casing and described lid are arranged to Split type structure, with handled easily person seal case.
Be beneficial to the cell transportation device maintaining cytoactive described in the present embodiment, using method is as follows:
Before cell transport, turn on inoculation seal cover 46, cell suspension is seeded in cavity volume 41 by cell inoculation mouth 43, screws up inoculation seal cover 46.Cell culture apparatus 4 is taken out from storage storage tank 3, inoculation seal cover 46 is unscrewed, is placed in the CO of 37 DEG C
2incubator carries out adhere-wall culture 24 hours.After cell attachment, cell culture apparatus 4 is positioned over and stores in storage tank 3.By CO
2gas tube and CO
2gas filling bottle connects (or connecting source of the gas), opens CO
2ventilating control valve 442, starts to fill CO in cavity volume 41
2.Treat CO
2after concentrations detectors 45 detection reaches requirement concentration, close CO
2ventilating control valve 442; CO
2filter 443 effectively can block CO
2in contained bacterial micro-organism, guarantee to keep germ-free condition in cavity volume 41.Afterwards, turning on low-temperature receiver seal cover 13, in cavity 11, pour into ice cube 6, to filling, screwing up low-temperature receiver seal cover 13.Lid 2 is fastened from top and casing 1.After arriving destination, cell culture apparatus 4 can be taken out from storage storage tank 3, after alcohol wipe outer surface, inoculation seal cover 46 be unscrewed, be directly placed in the CO of 37 DEG C
2incubator is cultivated, and row Secondary Culture again when cell state recovers completely, cell traffic process is impaired little, is beneficial to quick growth.
Scope of the present utility model is not by the restriction of described specific embodiments, and described embodiment only for as the single example of illustrating the utility model various aspects, also comprises method and the component of functional equivalent within the scope of the utility model.In fact, except content described herein, those skilled in the art can easily grasp multiple improvement of the present utility model with reference to description above and accompanying drawing.Described improvement also falls in the scope of appended claims.
Claims (8)
1. be beneficial to the cell transportation device maintaining cytoactive, comprise casing and cover the lid on described casing, it is characterized in that: in described casing top, be arranged with storage storage tank, in described storage storage tank, be removably connected with cell culture apparatus; Described bottom half and sidepiece are communicated with the cavity be provided with for holding low-temperature receiver, and described casing top is provided with at least one low-temperature receiver inlet being communicated with described cavity, and described low-temperature receiver inlet is sealed with low-temperature receiver seal cover; Described cell culture apparatus comprises and has cavity volume for holding the housing of cell suspension, and described housing upper is respectively equipped with cell inoculation mouth, CO
2aeration filtering device and CO
2concentrations detectors.
2. be beneficial to the cell transportation device maintaining cytoactive as claimed in claim 1, it is characterized in that: described CO
2aeration filtering device comprises CO
2gas tube, described CO
2gas tube one end is stretched in the cavity volume of described housing, and the other end is provided with CO
2ventilating control valve, described CO
2gas tube is positioned at described CO
2the inner side of ventilating control valve is provided with CO
2filter.
3. be beneficial to the cell transportation device maintaining cytoactive as claimed in claim 2, it is characterized in that: the outer wall of described casing is coated is provided with insulating.
4. be beneficial to the cell transportation device maintaining cytoactive as claimed in claim 3, it is characterized in that: described insulating is alumina silicate heat-insulation layer.
5. the cell transportation device being beneficial to maintenance cytoactive as described in any one of Claims 1-4 claim, is characterized in that: the cross sectional shape of described casing is rectangle or square.
6. be beneficial to the cell transportation device maintaining cytoactive as claimed in claim 5, it is characterized in that: described casing and described lid are by the hinged setting of jointed shaft.
7. the cell transportation device being beneficial to maintenance cytoactive as described in any one of Claims 1-4 claim, is characterized in that: the cross sectional shape of described casing is for circular or oval.
8. be beneficial to the cell transportation device maintaining cytoactive as claimed in claim 7, it is characterized in that: described casing is connected by thread seal with described lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420671357.8U CN204232156U (en) | 2014-11-11 | 2014-11-11 | Be beneficial to the cell transportation device maintaining cytoactive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420671357.8U CN204232156U (en) | 2014-11-11 | 2014-11-11 | Be beneficial to the cell transportation device maintaining cytoactive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204232156U true CN204232156U (en) | 2015-04-01 |
Family
ID=52761735
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420671357.8U Active CN204232156U (en) | 2014-11-11 | 2014-11-11 | Be beneficial to the cell transportation device maintaining cytoactive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204232156U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244415A (en) * | 2015-06-04 | 2016-12-21 | 泷源制造株式会社 | Cell, tissue are transported with without swinging housing |
-
2014
- 2014-11-11 CN CN201420671357.8U patent/CN204232156U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244415A (en) * | 2015-06-04 | 2016-12-21 | 泷源制造株式会社 | Cell, tissue are transported with without swinging housing |
| CN110106065A (en) * | 2015-06-04 | 2019-08-09 | 泷源制造株式会社 | Cell, tissue are transported with without swing shell |
| CN106244415B (en) * | 2015-06-04 | 2020-05-19 | 泷源制造株式会社 | Swing-free case for conveying cell and tissue slice |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |