CN111269821A - Microorganism culture device and method - Google Patents
Microorganism culture device and method Download PDFInfo
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- CN111269821A CN111269821A CN202010214786.2A CN202010214786A CN111269821A CN 111269821 A CN111269821 A CN 111269821A CN 202010214786 A CN202010214786 A CN 202010214786A CN 111269821 A CN111269821 A CN 111269821A
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- 244000005700 microbiome Species 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 239000011229 interlayer Substances 0.000 claims abstract description 20
- 238000004891 communication Methods 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000009629 microbiological culture Methods 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- ARIWANIATODDMH-AWEZNQCLSA-N 1-lauroyl-sn-glycerol Chemical compound CCCCCCCCCCCC(=O)OC[C@@H](O)CO ARIWANIATODDMH-AWEZNQCLSA-N 0.000 claims description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 3
- ARIWANIATODDMH-UHFFFAOYSA-N Lauric acid monoglyceride Natural products CCCCCCCCCCCC(=O)OCC(O)CO ARIWANIATODDMH-UHFFFAOYSA-N 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 239000007864 aqueous solution Substances 0.000 description 9
- 239000007789 gas Substances 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 238000012136 culture method Methods 0.000 description 3
- 239000011790 ferrous sulphate Substances 0.000 description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000001146 hypoxic effect Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- Bioinformatics & Cheminformatics (AREA)
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- Sustainable Development (AREA)
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Abstract
The invention provides a microorganism culture device and a method, wherein the microorganism culture device comprises a shell and a cover body; the upper end of the hollow first structural member is open, and the lower end of the hollow first structural member is closed; the outer wall of the first structural member is provided with a downward first extension part; the upper end of the hollow second structural member is closed, and the lower end of the hollow second structural member is opened; the inner diameter of the second structural part is not less than the outer diameter of the first structural part, and the second structural part is buckled on the first structural part; a culture medium adapted to be disposed on an inner wall of the upper end of the second structure in an area in communication with the interlayer formed between the upright wall of the first structure and the upright wall of the second structure; the interlayer is adapted to contain an aqueous liquid which isolates the area from the outside; the open ends of the first extension part and the second structural part are connected with a fixing part arranged in the shell. The invention has the advantages of good culture effect and the like.
Description
Technical Field
The invention relates to microorganisms, in particular to a microorganism culture device and a method.
Background
Anaerobic microorganisms are a class of microorganisms that survive in hypoxic or anaerobic environments and are widely distributed in nature. At present, in China, no matter a detection department or an authoritative laboratory, an anaerobic incubator is used, the design is complex, the anaerobic incubator needs to be inflated and deflated for many times when being operated, the real-time observation cannot be carried out, and the size of the conventional anaerobic incubator is larger.
In addition, the air humidity has obvious influence on the metabolic activity of the microorganisms, and the culture humidity is generally required to be more than 80-90% except for the dried microorganisms. Under the culture environment of 35-37 ℃, water in the culture medium is easy to volatilize, even the culture medium is cracked, and the common method is to place a cup of water in the incubator to ensure the humidity required by the growth of bacteria. However, this method is prone to cause uneven distribution of moisture and humidity, resulting in variation between groups, and is prone to overturn.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides the microbial culture device with good culture effect.
The purpose of the invention is realized by the following technical scheme:
microorganism culture apparatus, microorganism culture apparatus includes casing and lid, microorganism culture apparatus still includes:
the upper end of the hollow first structural member is open, and the lower end of the hollow first structural member is closed; the outer wall of the first structural member is provided with a downward first extension part;
the upper end of the hollow second structural part is closed, and the lower end of the hollow second structural part is opened; the inner diameter of the second structural part is not less than the outer diameter of the first structural part, and the second structural part is buckled on the first structural part; a culture medium adapted to be disposed on an inner wall of the upper end of the second structure in an area in communication with the interlayer formed between the upright wall of the first structure and the upright wall of the second structure; the interlayer is adapted to contain an aqueous liquid which isolates the area from the outside;
the opening ends of the first extension part and the second structural part are connected with the fixing part arranged in the shell.
The invention also aims to provide a microorganism culture method with good culture effect, and the aim of the invention is realized by the following technical scheme:
the microorganism culture method of the microorganism culture apparatus comprises the following steps:
(A1) placing a culture medium on the bottom wall of the second structural member with the opening facing upwards;
(A2) buckling a second structural member outside the first structural member with an opening facing downwards, wherein the first structural member and the second structural member are respectively connected with the fixing part;
(A3) injecting water-containing liquid into the shell, wherein the water-containing liquid enters an interlayer between the first structural member and the second structural member, and the generated water vapor enters the area where the culture medium is located; the aqueous liquid isolates the area where the medium is located from the outside.
Compared with the prior art, the invention has the beneficial effects that:
1. the culture effect is good;
the environment of the culture medium is completely isolated from the outside: the liquid in the interlayer isolates the culture medium from the outside, so that the culture medium can be effectively isolated from oxygen, the requirement on a vacuum environment is reduced, and the culture effect is improved;
furthermore, the water-containing liquid absorbs oxygen in the gas in the environment where the interlayer and the culture medium are located, so that the culture effect is further improved;
in addition, the bottom wall of the shell is heated, and heat and moisture humidity are provided by using liquid, so that the temperature and humidity are distributed more uniformly, and the environmental difference is reduced;
2. the structure is simple;
the humidity of the environment where the culture medium is located is adjusted by utilizing the water-containing liquid, the liquid contains a humidity regulator, a reducing agent and the like, the internal gas components can be effectively adjusted, stable moisture humidity is provided, and a complex water vapor providing device in the prior art is replaced.
Drawings
The disclosure of the present invention will become more readily understood with reference to the accompanying drawings. As is readily understood by those skilled in the art: these drawings are only for illustrating the technical solutions of the present invention and are not intended to limit the scope of the present invention. In the figure:
FIG. 1 is a schematic view of the construction of a microorganism culture apparatus according to example 1 of the present invention;
FIG. 2 is a schematic longitudinal sectional view of a part of a microorganism culture apparatus according to example 1 of the present invention;
FIG. 3 is a schematic longitudinal sectional view of a microorganism culture apparatus according to example 2 of the present invention;
FIG. 4 is a schematic cross-sectional view of a microorganism culture apparatus according to example 3 of the present invention.
Detailed Description
Fig. 1-4 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and use the invention. Some conventional aspects have been simplified or omitted for the purpose of teaching the present invention. Those skilled in the art will appreciate that variations or substitutions from these embodiments will be within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Thus, the present invention is not limited to the following alternative embodiments, but is only limited by the claims and their equivalents.
Example 1:
FIGS. 1 to 2 are schematic views showing the construction of a microorganism culture apparatus according to example 1 of the present invention, as shown in FIGS. 1 to 2, comprising:
the housing 11 and the cover 31, which are prior art in the field, and the detailed structure is not described herein;
a first structural member 41 having an open upper end and a closed lower end, the first structural member 41 being hollow; the outer wall of the first structural member 41 has a first extension 412 extending downward, e.g., the first extension 412 extends downward parallel to the central axis direction of the first structural member 41;
a second structural member 42, the upper end of the hollow second structural member 42 is closed, and the lower end is open; the inner diameter of the second structural member 42 is not smaller than the outer diameter of the first structural member 41, and the second structural member is buckled on the first structural member 41; a culture medium 61 is adapted to be arranged on the inner wall of the upper end of the second structure 42, said culture medium 61 being located in an area communicating with an interlayer formed between the standing wall 411 of the first structure 41 and the standing wall 421 of the second structure 42; a gap is formed between the bottom end of the second structural member 42 and the bottom wall of the shell 11;
the fixed parts 51-52, the open ends of the first extension part 412 and the second structural part 42 are connected with the fixed parts 51-52 arranged in the shell; in the embodiment, the fixing part adopts a plurality of rubber pieces, the bottom end of the fixing part is fixed on the bottom wall of the shell, and the upper end of the fixing part is provided with a groove which is suitable for the open ends of the first extension part and the second structural part to be clamped and fixed;
an aqueous liquid, wherein the liquid contains ferrous sulfate and/or sodium chloride, and the solvent is water; the shell is internally provided with an aqueous liquid which enters the interlayer, so that water vapor enters the area where the culture medium is positioned, and the culture is maintained in a proper humidity environment; the liquid is only in the space inside the interlayer and outside the second structural member, and the interlayer is communicated with the space;
the heater heats the bottom wall of the shell, so that liquid in the shell is heated, water vapor enters the area where the culture medium is located, and meanwhile, a certain temperature is maintained.
According to the working method of the microorganism culture apparatus of the embodiment of the present invention, the microorganism culture method comprises the steps of:
(A1) placing a culture medium on the bottom wall of the second structural member with the opening facing upwards;
(A2) detain second structure opening ground outside first structure, first structure and second structure are connected with the fixed part respectively, specifically are: the opening ends of the first extension part and the second structural part are clamped into the upper end groove of the fixing part and are prevented from loosening by virtue of friction force;
(A3) and injecting an aqueous solution into the shell, wherein the aqueous solution enters the interlayer between the first structural member and the second structural member, and the generated water vapor enters the area where the culture medium is positioned, so that the fixed humidity is maintained by means of the preparation of the aqueous solution.
Example 2:
the microorganism culture apparatus of example 2 of the present invention is different from example 1 in that:
as shown in fig. 3, the inner wall of the second structure 42 has a downward second extending portion 422, and the second extending portion 422, the vertical wall 411 of the first structure 41 and the vertical wall 421 of the second structure are sequentially arranged from inside to outside; the bottom end of the second extension 422 is lower than the top end of the vertical wall 411 of the first structural member 41, so that the area where the culture medium is located is still communicated with the interlayer, and moisture in the interlayer enters the area where the culture medium is located.
Example 3:
the microorganism culture apparatus of example 3 of the present invention is different from example 1 in that:
as shown in fig. 4, the fixing portion is formed by a plurality of rubber columns 51-52 separated from each other and fixed on the bottom wall in the housing, so that the first extension portion 412 is clamped outside the rubber column 52, and the lower end opening of the upright wall 42142 of the second structural member is clamped inside the rubber column.
Example 4:
an application example of the microorganism culture apparatus and method according to embodiment 1 of the present invention to culture of facultative anaerobes.
In this application, the housing has a liquid inlet and outlet.
A method of culturing a microorganism comprising the steps of:
(A1) placing a culture medium on the bottom wall of the second structural member with the opening facing upwards;
(A2) detain second structure opening ground outside first structure, first structure and second structure are connected with the fixed part respectively, specifically are: the opening ends of the first extension part and the second structural part are clamped into the upper end groove of the fixing part and are prevented from loosening by virtue of friction force; the cover body is buckled on the shell, so that a closed space is enclosed by the cover body and the shell;
(A3) opening a liquid inlet, injecting aqueous solution into the shell, introducing the aqueous solution into an interlayer between the first structural member and the second structural member, introducing generated water vapor into the area where the culture medium is located, and maintaining fixed humidity by virtue of preparation of the aqueous solution; meanwhile, a small amount of oxygen in the area where the culture medium is located is absorbed by the aqueous liquid; the aqueous liquid is prepared in the following way:
weighing 10-ferrous sulfate and 50-100 g of sodium chloride, dissolving in 1L of water, adding 20-100 mg of glycerol monolaurate, homogenizing at 800-1200 r/min for 1min1, and adding 10-50 g of iron powder.
Example 5:
an application example of the microorganism culture apparatus and method according to embodiment 1 of the present invention to the culture of obligate anaerobes.
In this application, the housing has a liquid inlet and outlet and a gas inlet and outlet.
A method of culturing a microorganism comprising the steps of:
(A1) placing a culture medium on the bottom wall of the second structural member with the opening facing upwards;
(A2) detain second structure opening ground outside first structure, first structure and second structure are connected with the fixed part respectively, specifically are: the opening ends of the first extension part and the second structural part are clamped into the upper end groove of the fixing part and are prevented from loosening by virtue of friction force; the cover body is buckled on the shell, so that a closed space is enclosed by the cover body and the shell;
(A3) closing the gas inlet, the liquid inlet and the liquid outlet, and vacuumizing the closed space by using the gas outlet;
closing the gas outlet, and introducing one or more gases of CO2, nitrogen and hydrogen by using the gas inlet;
opening a liquid inlet, injecting aqueous solution into the shell, introducing the aqueous solution into an interlayer between the first structural member and the second structural member, introducing generated water vapor into the area where the culture medium is located, and maintaining fixed humidity by virtue of preparation of the aqueous solution; meanwhile, a small amount of oxygen in the area where the culture medium is located is absorbed by the aqueous liquid; the aqueous liquid is prepared in the following way:
weighing 10-ferrous sulfate and 50-100 g of sodium chloride, dissolving in 1L of water, adding 20-100 mg of glycerol monolaurate, homogenizing at 800-1200 r/min for 1min1, and adding 10-50 g of iron powder.
Claims (9)
1. Microorganism culture apparatus, microorganism culture apparatus includes casing and lid, its characterized in that: the microbial cultivation apparatus further includes:
the upper end of the hollow first structural member is open, and the lower end of the hollow first structural member is closed; the outer wall of the first structural member is provided with a downward first extension part;
the upper end of the hollow second structural part is closed, and the lower end of the hollow second structural part is opened; the inner diameter of the second structural part is not less than the outer diameter of the first structural part, and the second structural part is buckled on the first structural part; a culture medium adapted to be disposed on an inner wall of the upper end of the second structure in an area in communication with the interlayer formed between the upright wall of the first structure and the upright wall of the second structure; the interlayer is adapted to contain an aqueous liquid which isolates the area from the outside;
the opening ends of the first extension part and the second structural part are connected with the fixing part arranged in the shell.
2. The microbial culture apparatus of claim 1, wherein: the interlayer is communicated with the space outside the second structural member;
the bottom wall of the housing has a heater.
3. The microbial culture apparatus of claim 1, wherein: the inner wall of the second structural member is provided with a downward second extension part, and the second extension part, the first structural member and the second structural member are sequentially arranged from inside to outside.
4. The microbial culture apparatus of claim 3, wherein: the bottom end position of the second extension part is lower than the top end position of the first structural member.
5. The microbial culture apparatus of claim 1, wherein: the fixing part is provided with a groove, and the lower ends of the first extending part and the second structural part are suitable for being clamped in; a gap is formed between the bottom end of the second structural member and the bottom wall of the shell.
6. The microbial culture apparatus of claim 1, wherein: the fixing parts are made of flexible materials, and the lower ends of the first extension parts and/or the second structural parts are clamped between the fixing parts; or the fixing part is clamped inside the lower end of the first extending part and/or the second structural part.
7. The method for culturing microorganisms in the microorganism culture apparatus according to any one of claims 1 to 6, wherein the method comprises:
(A1) placing a culture medium on the inner wall of the second structural member;
(A2) buckling a second structural member outside the first structural member with an opening facing downwards, wherein the first structural member and the second structural member are respectively connected with the fixing part;
(A3) injecting water-containing liquid into the shell, wherein the water-containing liquid enters an interlayer between the first structural member and the second structural member, and the generated water vapor enters the area where the culture medium is located; the aqueous liquid isolates the area where the medium is located from the outside.
8. The method for culturing a microorganism according to claim 7, wherein: the oxygen in the region reacts with species in the liquid.
9. The method for culturing a microorganism according to claim 7, wherein: the liquid has iron powder and glycerol monolaurate.
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| CN202010214786.2A CN111269821B (en) | 2020-03-24 | 2020-03-24 | Microorganism culture device and method |
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| CN202010214786.2A CN111269821B (en) | 2020-03-24 | 2020-03-24 | Microorganism culture device and method |
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Citations (8)
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| JP2006254790A (en) * | 2005-03-17 | 2006-09-28 | Kubota Corp | Culture medium for anaerobic microorganism and culturing method |
| CN102021113A (en) * | 2010-09-21 | 2011-04-20 | 钱正浩 | Portable anaerobic microorganism culturing device |
| CN105385582A (en) * | 2015-12-21 | 2016-03-09 | 山东科技大学 | Novel anaerobic-bacterium culturing dish |
| CN206157150U (en) * | 2016-10-27 | 2017-05-10 | 山东科技大学 | Solid intermediate layer culture dish of anaerobic microorganisms separation |
| CN208293014U (en) * | 2018-04-20 | 2018-12-28 | 珠海京工检测技术有限公司 | A kind of middle-size and small-size oxygen consumption microbial cultivation device |
| CN109825425A (en) * | 2019-02-19 | 2019-05-31 | 江苏中新医药有限公司 | A kind of air-tightness culture dish of generation and maintenance anaerobic or micro- oxygen microenvironment |
| CN208964929U (en) * | 2018-10-19 | 2019-06-11 | 四川农业大学 | A kind of anaerobe experiment culture apparatus |
| CN110760440A (en) * | 2019-12-03 | 2020-02-07 | 焦作大学 | Culture apparatus of anaerobic microorganism |
-
2020
- 2020-03-24 CN CN202010214786.2A patent/CN111269821B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006254790A (en) * | 2005-03-17 | 2006-09-28 | Kubota Corp | Culture medium for anaerobic microorganism and culturing method |
| CN102021113A (en) * | 2010-09-21 | 2011-04-20 | 钱正浩 | Portable anaerobic microorganism culturing device |
| CN105385582A (en) * | 2015-12-21 | 2016-03-09 | 山东科技大学 | Novel anaerobic-bacterium culturing dish |
| CN206157150U (en) * | 2016-10-27 | 2017-05-10 | 山东科技大学 | Solid intermediate layer culture dish of anaerobic microorganisms separation |
| CN208293014U (en) * | 2018-04-20 | 2018-12-28 | 珠海京工检测技术有限公司 | A kind of middle-size and small-size oxygen consumption microbial cultivation device |
| CN208964929U (en) * | 2018-10-19 | 2019-06-11 | 四川农业大学 | A kind of anaerobe experiment culture apparatus |
| CN109825425A (en) * | 2019-02-19 | 2019-05-31 | 江苏中新医药有限公司 | A kind of air-tightness culture dish of generation and maintenance anaerobic or micro- oxygen microenvironment |
| CN110760440A (en) * | 2019-12-03 | 2020-02-07 | 焦作大学 | Culture apparatus of anaerobic microorganism |
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