CN204981851U - Rotary bioreactor - Google Patents

Rotary bioreactor Download PDF

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Publication number
CN204981851U
CN204981851U CN201520729703.8U CN201520729703U CN204981851U CN 204981851 U CN204981851 U CN 204981851U CN 201520729703 U CN201520729703 U CN 201520729703U CN 204981851 U CN204981851 U CN 204981851U
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base
cell culture
rotary bioreactor
culture case
box
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CN201520729703.8U
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曾宪卓
曾明哲
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ISTEM REGENERATIVE MEDICINE SCI-TECH Co Ltd
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Abstract

The utility model provides a rotary bioreactor, including have the base that holds the concave part and with the top cap of this base looks adaptation, the base hold the concave part in be equipped with the detachable cell culture box, and have the cell culture space of rank ladder shape in the cell culture box, still be equipped with on the base and be used for driving this base and use the base axis and carry out the drive arrangement of rotation as the axle. Adopt the utility model discloses an above -mentioned rotary bioreactor compares conventional fermentation cylinder, can satisfy different individualized individual cell culture's a requirement through training the box, and the cultivation process from in a small amount to a large amount of excessively. After above -mentioned structural change, it can greatly reduced to compare on the whole in the stainless steel fermentation cylinder weight of common integral type, drive arrangement such as motor through the conventionality or motor alright in order to make the base carry out the rotation stirring very easily, the cell of having avoided conventional mechanical shock that mode such as turbine formula whirl or stirring rod stirring produced the fermentation cylinder for and shearing force to cause harms.

Description

Rotary bioreactor
Technical field
The utility model belongs to biotechnology equipment technical field, is specifically related to a kind of rotary bioreactor.
Background technology
Bio-reactor is used to carry out device the most frequently used in microorganism culturing, fermentation; Its main body is generally the main formula cylinder made with stainless steel plate, and its volume is at 1m 3to hundreds of m 3.When adopting these common bio-reactors existing to carry out microorganism culturing, the space in reactor is homogeneous form, is all directly to carry out mass propgation after microbe inoculation bacterial classification in reactor.But the initial stage cell concentration of a lot of cell cultures is little, has requirement again to culture density, and common bio-reactor not too can realize cultivating the excessive such function of mass propgation in a small amount simultaneously.
Simultaneously, the container portions of existing common bio-reactor is all by the one-body molded preparation of stainless steel, sterilization, sterilizing and quality inspection etc. operate inconvenience on the one hand, on the other hand due to its overall volume and weight huge, inner stirring can only adopt turbine type eddy flow, the mode of air lift type or stirring rod and so on is carried out, shearing force and the mechanical impact of the increase in stirring easily cause cell injury, and the low angle three-dimensional rotation can not carrying out being best suited for Growth of Cells stirs.
Utility model content
The object that the utility model is implemented is the above-mentioned deficiency overcoming prior art, provide a kind of can from the rotary bioreactor in a small amount to a large amount of excessive cultivations.
In order to realize above-mentioned utility model object, the technical scheme of the utility model embodiment is as follows:
A kind of rotary bioreactor, comprises the base and the top cover suitable with this base that have and hold recess; Be provided with dismountable cell culture case in the accommodation recess of described base, and in this cell culture case, there is the cell culturing space of ladder cylindricality;
Described base is also provided with for driving this base with base center axis line for axle carries out the drive unit of rotation.
Adopt above-mentioned rotary bioreactor of the present utility model, compare conventional fermentor tank, cultivate box by above-mentioned ladder cylindricality and can meet the requirement that different personalized individual cells cultivates, and culturing process is from a small amount to a large amount of excessive.After said structure changes, the stainless steel fermentor tank weight comparing common integral type on the whole can reduce greatly, just base can be made easily to carry out rotation stirring by the drive unit such as motor or motor of routine, the mechanical shock that the modes such as normal fermentation tank turbine type eddy flow or stirring rod stirring that avoid produce and the cell injury that shearing force causes.
Preferably, the inner-wall surface that described base holds recess is provided with draw-in groove, and the outside surface of described cell culture case is provided with the card column with this draw-in groove adaptation.
Preferably, in described accommodation recess, be also provided with the heating in water bath part of spill, and on the inner-wall surface of the concave shaped space of this heating in water bath part with the external shape fits of cell culture case; Described cell culture case is placed in the concave shaped space of described heating in water bath part.
Preferably, described heating in water bath part and base are wholely set.
Preferably, described cell culture case is provided with air inlet port relative to the surface of top cover, and described top cover is provided with the inflation needle with described air inlet port adaptation;
And/or described cell culture case is provided with the liquid filling hole for adding nutrient solution relative to the surface of top cover, described top cover is provided with the liquid feeding syringe needle with described liquid filling hole adaptation.
Preferably, described rotary bioreactor also comprises the stopple coupon be through to from described base outer surface in cell culture case.
Preferably, described stopple coupon is provided with the electric control valve for opening and closing this stopple coupon.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model is described in further detail, in accompanying drawing:
Fig. 1 is the structural representation of the utility model embodiment rotary bioreactor;
Fig. 2 is the structural representation of the cultivation box shown in Fig. 1.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.
The utility model example provides a kind of rotary bioreactor, shown in Figure 1, comprise the spill base 10 and suitable with this base 10 and for covering base 10 top cover 20 with accommodation recess (installed cultivation box 30 owing to holding in recess in figure, therefore do not indicated separately).
Normal and common design based on the comparison, the profile of base 10 can adopt conventional columniform shape, and the profile of such fermentor tank, than compared with normal, compares other improper form, and this cylinder form also compares is convenient to process for processing.The cultivation box 30 for culturing cell is provided with in the accommodation recess of base 10; Certainly, this cultivation box 30 is fixed on by dismountable mode and holds in recess, simultaneously for the ease of the shape adaptation of the shape of stable cultivation box 30 and the accommodation recess of base 10, compare the uncomfortable timing of shape to need with fixed connecting piece by cultivating the firm situation of box 30, the cost of parts and process for processing cost that adopt fixed connecting piece can be reduced.And the cultivation box 30 adopted in this case, its axial cross section shape can be shown in Figure 1, the culture space of cultivating box 30 inner hollow is along with the diameter of column part increases and the ladder cylindrical shape of increase (the trend axially stacked shape formed namely increased gradually by diameter by multiple cylindricalitys), after the amount of adding different substratum, the space spaciousness degree that the cell of different positions has is not identical, especially the part that bottommost post diameters is minimum, the space that its cell has and density maximum, so control as required to add different cultivation liquid measures at different times (but such as commitment cell concentration is little has requirement to culture density again), can realize from excessive to carrying out in a large number in a small amount.
And do not adopt fastening connection piece based on this practicality above-mentioned and directly adopt shape adaptation to carry out cultivating the fixing situation of box 30, the cell culturing space shape of cultivating in box 30 is ladder cylindricality, profile so based on this cultivation box 30 can be arbitrary shape, but produce and the structure of design and box based on normal, this practicality is corresponding is also designed to the ladder cylindricality identical with interior culture space shape by the profile of cultivating box, its profile specifically can be shown in Figure 2, cultivates wall thickness about the 4cm of box 30.Simultaneously, be mounted in the accommodation recess of base 10 owing to cultivating box 30, so can adopt by the accommodation recess inner-wall surface shape of base 10 also corresponding in suitable ladder cylindrical shape (if the profile of cultivating box 30 does not adopt above-mentioned preferred ladder cylindricality, so the accommodation recess of base 10 also correspondence can change; And the mode of this shape adaptation is arranged in the accommodation recess of base 10 in order to what make that cultivation box 30 can be comparatively stable, if shape is not adaptive, so auxiliary connection fastening pieces will be cultivated box 30 and fix, just easy not as the mounting means of above-mentioned shape adaptation), certainly if square column type or Polygonal column shape, the profile of cultivating box 30 after installation is automatically formed with accommodation recess and is fixed; If stepped cylindrical shape, after so installing, in the process of cultivating, (in stirring, concussion etc. process) cultivation box 30 can be unstable from transferring, so can assist the structure some being set and being convenient to fix, on the outside surface cultivating box 30, such as arrange along the card column of cultivating box 30 axis, the corresponding inner-wall surface holding recess arrange draw-in groove that (fixed sturcture of card column, draw-in groove is comparatively common fixed sturcture, so do not carry out indicating and embodying in the accompanying drawing of this case), thus make the fastening of cultivation box 30.Certainly, the mode that above-mentioned post groove is fixing in theory can be arranged on the contrary, the outside surface cultivating box 30 arranges draw-in groove, and the inner-wall surface holding recess arranges card column, but it is thinner on the wall due to cultivation box 30, the stability of cultivating box 30 can be reduced, so do not adopt this kind of mode to carry out in implementing as far as possible after draw-in groove is set.
When adopting the cultivation box 30 of this dismountable ladder cylindricality to carry out cell cultures, this cultivation box 30 is when good seal, can take apart separately after having carried out cell fermentation cultivation each time and carry out cleaning and sterilizing or directly change, the fermentor tank comparing the one-body molded preparation of existing common stainless steel is obviously convenient to the operations such as cleaning, sterilization, sterilizing and quality inspection more.And the fermentor tank of existing routine is when carrying out fermentation culture to the cell of some singularity, such as some metabolites are in acid cell, its acidic metabolite can produce acid attack to stainless inwall, and cause metal ion to dissolve in nutrient solution, these metal ions have some be have Cytotoxic; And when adopting bio-reactor of the present utility model to carry out, according to the different growth requirement of cytotoxicity and cellular affinity, can the cultivation box 30 of differing materials be correspondingly adopted to carry out, when cultivate be the bacterium of above-mentioned acidic metabolite time, the cultivation box 30 that fluoropolymer resin (such as polystyrene material) so just can be adopted to make is cultivated; If some cells needing biocompatible environment well to grow, can carry out by the corresponding cultivation box 30 adopting the biological materials such as chitosan to prepare; Thus realize the requirement meeting different personalized individual cells cultivation.
After adopting the structure of the above-mentioned basic fermentor tank of this practicality, its material and structure are all unnecessary as common stainless steel, base 10 and top cover 20 directly do not contact the bacterium of cultivation, so just, the base 10 of some light-weight metals or hollow sandwich and top cover 20 can be adopted to carry out, after weight reduces greatly, just drive unit 40 can be set in the bottom of base 10, drive it carry out rotation with the axis of cylindrical base 10 for axle thus realize the stirring of cell; Drive unit 40 can realize with conventional motor, motor etc.Such rotation is stirred and is more close to low angle three-dimensional rotation and stirs, and avoids mechanical shock that mode that normal fermentation tank turbine type eddy flow, air lift type or stirring rod stir produces cell and the cell injury that shearing force causes.
Further on the basis of above-mentioned embodiment, the space of above-mentioned cell cultures mainly in cultivation box 30 of this practicality is carried out, its internal space and base 10 separate, and adopts and be directly not too suitable for this kind of situation to the temperature measurement and control that tank body carries out in conventional fermentor tank; Based on this purpose, between cultivation box 30 and accommodation recess, be provided with a heating in water bath part 50; By this heating in water bath part 50, cultivation box 30 heated and preserved, making the homo(io)thermism of its inside.Based on enforceable mode, the shape of this heating in water bath part 50 can be carried out by reference base 10, adopts the design of spill proterties; In the process implemented, above-mentioned cultivation box 30 is placed in the concave shaped space of this heating in water bath part 50, is controlled the temperature in fermentation culture process by the water bath of constant temperature (or alternating temperature).Certainly, based on the mode of this kind of enforcement, in order to the respond well of temperature conduction will be made, cultivation box 30 so can be made to be close in the concave shaped space of heating in water bath part 50, adopt in enforcement and the concave shaped space of heating in water bath part 50 is designed to and the ladder post external shape fits cultivating box 30, then cultivation box 30 is placed in this heating in water bath part 50.
Certainly, known with the fixed form of the accommodation recess of base 10 based on above-mentioned cultivation box 30, the surface may cultivating box 30 have some fixed sturctures (such as card column or connect fastening piece), so in the process of above-mentioned adaptation, the concave shaped space of heating in water bath part 50 is with when cultivating box 30 adaptation, be designed to can for these fixed sturctures through through hole etc., like this can be good fit.
And further, because Heating Water part 50 itself also needs to be fixed in base 10, so still also can fixed connecting piece be adopted, or by the proterties identical adaptation of the configuration design one-tenth of Heating Water part 50 with the accommodation recess of base 10, so direct being placed on by Heating Water part 50 can be simply fixing in base 10.Due in the process implemented, with metal material preparation in the process that this heating in water bath part 50 uses, to facilitate heat conduction, simultaneously because it does not need dismounting, so this heating in water bath part 50 can adopt directly make one with base 10, more stablize, there will not be problems such as loosening wearing and tearing.
The mode of heating in water bath part 50 self-heating is realized due to employing heating circuit cost and supporting power equipment, be unfavorable for implementing in the base 10 closed, so based on above-mentioned purpose, preferably adopt in this case and heating in water bath part 50 design itself is become hollow structure, water storage is used in hollow, on heating in water bath part 50, offer water-in and water outlet simultaneously, and in base 10, the inlet channel docked with water outlet and water-in and outlet conduit are set, just water outlet and fill cycle (as illustrated by the arrows in fig. 1) can be formed in heating in water bath part 50 afterwards, the water being filled with differing temps is required according to the differing temps in cell cultivation process, just good temperature control effect can be realized.
In the foundation structure had at above-mentioned bio-reactor further, in order to further be convenient to the operations such as reinforced, ventilation in culturing process, the surface that above-mentioned cultivation box 30 top is relative with top cover 20 offers and adds pore 31 and liquid filling hole 32, these two holes corresponding simultaneously, the surface that top cover 20 is relative with cultivating box 30 arranges aerating syringe needle 21 and liquid feeding syringe needle 22.Aerating syringe needle 21 aligning of the back header 20 of whole fermentor tank installation adds pore 31, liquid feeding syringe needle 22 closes top cover 20 after aiming at liquid filling hole 32; Then as similarly in usual fermentor tank be connected aerating syringe needle 21 and liquid feeding syringe needle 22 with tracheae with liquid pipe, aerating syringe needle and gas concentration lwevel induction and humidity sensor just can be utilized afterwards to control gas concentration lwevel in reaction vessel and humidity; And in cultivation box 30, add supplemental medium by liquid feeding syringe needle 22.Certainly, in the process implemented, this liquid feeding syringe needle 22 will adopt needles with two holes, and a hole on syringe needle is used for liquid feeding, and another hole is used for exhausted air while liquid feeding, cultivates the air pressure in box 30, prevent high pressure from bursting in order to balance.Further, wanting seal itself and adaptive with syringe needle based on adding pore 31 and liquid filling hole 32, so memory rubber hole can be adopted to prepare in the process implemented, comparing the circumstances dictate meeting this use.
Meanwhile, further for the ease of the cell sampling in fermentation culture process and detection, the utility model rotary bioreactor also comprises one from base 10, is through to the stopple coupon 11 cultivated in box 30; Certainly, for the ease of normal sampling operation can be beneficial to, cultivates box 30 and can be opened in the bottom position cultivating box 30 for the through hole that stopple coupon 11 passes.Further, because stopple coupon 11 is only opened to carry out the operations such as sampling, so arrange a by-pass valve control (this is comparatively common parts, so this case is attached not shown) thereon when needs; Control opening or closing of stopple coupon 11, valve-off in the process of usually cultivating; And when needs sampling waits operation, open valve and make stopple coupon 11 conducting carry out the operations such as sampling.
In the above-described embodiment, top cover 20 self can adopt the hemispherical dome structure of hollow to carry out, inner hollow is used for arranging the conduit component of above-mentioned liquid feeding and aerating, outer thus cause the problems such as the winding easily produced in Stirring process to avoid conduit component to be arranged on rotary bioreactor.
Adopt above-mentioned rotary bioreactor in the process of carrying out cell fermentation cultivation, the operating process completed can according to fixing each parts of correspondence position assembling such as first corresponding above-mentioned pin, holes.Then, by stopple coupon 11, initial incubation liquid and cell are pumped in cultivation box 30, open switch, start cultivation operation according to setting program.In the process of cultivating, carry out gas concentration lwevel, humidity by aerating syringe needle 21 and liquid feeding syringe needle 22 and cultivate interpolation and the control of liquid measure; Counting cells can also be sampled by stopple coupon 11 at any time simultaneously, implement to obtain cell density etc. and be convenient to control the change of cultivation situation; Fermentation culture product is collected by stopple coupon 11 after cultivation completes.
Adopt above-mentioned rotary bioreactor of the present utility model, compare conventional fermentor tank, cultivate box by above-mentioned ladder cylindricality and can meet the requirement that different personalized individual cells cultivates, and culturing process is from a small amount to a large amount of excessive.After said structure changes, the stainless steel fermentor tank weight comparing common integral type on the whole can reduce greatly, just base can be made easily to carry out rotation stirring by the drive unit such as motor or motor of routine, the mechanical shock that the modes such as normal fermentation tank turbine type eddy flow or stirring rod stirring that avoid produce and the cell injury that shearing force causes.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.

Claims (7)

1. a rotary bioreactor, comprises the base and the top cover suitable with this base that have and hold recess; It is characterized in that, be provided with dismountable cell culture case in the accommodation recess of described base, and in this cell culture case, there is the cell culturing space of ladder cylindricality;
Described base is also provided with for driving this base with base center axis line for axle carries out the drive unit of rotation.
2. rotary bioreactor as claimed in claim 1, is characterized in that, the inner-wall surface that described base holds recess is provided with draw-in groove, and the outside surface of described cell culture case is provided with the card column with this draw-in groove adaptation.
3. rotary bioreactor as claimed in claim 1 or 2, is characterized in that, be also provided with the heating in water bath part of spill in described accommodation recess, and the concave shape of this heating in water bath part and the external shape fits of cell culture case;
Described cell culture case is placed in the concave shaped space of described heating in water bath part.
4. rotary bioreactor as claimed in claim 3, it is characterized in that, described heating in water bath part and base are wholely set.
5. rotary bioreactor as claimed in claim 1 or 2, it is characterized in that, described cell culture case is provided with air inlet port relative to the surface of top cover, and described top cover is provided with the inflation needle with described air inlet port adaptation;
And/or described cell culture case is provided with the liquid filling hole for adding nutrient solution relative to the surface of top cover, described top cover is provided with the liquid feeding syringe needle with described liquid filling hole adaptation.
6. rotary bioreactor as claimed in claim 1 or 2, is characterized in that, described rotary bioreactor also comprises the stopple coupon be through to from described base outer surface in cell culture case.
7. rotary bioreactor as claimed in claim 6, is characterized in that, described stopple coupon being provided with the electric control valve for opening and closing this stopple coupon.
CN201520729703.8U 2015-09-18 2015-09-18 Rotary bioreactor Active CN204981851U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434286A (en) * 2016-11-30 2017-02-22 黑龙江省能源环境研究院 Rotary anaerobic fermentation gas collector and using method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434286A (en) * 2016-11-30 2017-02-22 黑龙江省能源环境研究院 Rotary anaerobic fermentation gas collector and using method thereof

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C14 Grant of patent or utility model
GR01 Patent grant
C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20160223

Address after: 518057, Guangdong Province, Nanshan District high tech Zone, South Ring Road, No. 29, No. 02 building, international students, building No. 15

Patentee after: Zeng Xianzhuo

Address before: 518057, Guangdong Province, Nanshan District high tech Zone, South Ring Road, No. 29, No. 02 building, international students, building No. 15

Patentee before: ISTEM REGENERATIVE MEDICINE SCI-TECH CO., LTD.

C56 Change in the name or address of the patentee
CP02 Change in the address of a patent holder

Address after: 518057, room 2, building 10, Shenzhen biological incubation center, No. 302, Nanshan District hi tech, Shenzhen, Guangdong

Patentee after: Zeng Xianzhuo

Address before: 518057, Guangdong Province, Nanshan District high tech Zone, South Ring Road, No. 29, No. 02 building, international students, building No. 15

Patentee before: Zeng Xianzhuo