CN102021113A - Portable anaerobic microorganism culturing device - Google Patents
Portable anaerobic microorganism culturing device Download PDFInfo
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Abstract
The invention discloses a portable anaerobic microorganism culturing device, which comprises a test tube and a seal cover used for sealing the test tube mouth, wherein an inner packed column is arranged inside the test tube, and a closed space formed by the seal cover, the inner packed column and the test tube is filled with an anaerobic microorganism culturing medium. In the technical scheme, the anaerobic microorganism culturing medium is implanted into a sandwiched space between the test tube and the inner packed column, and the closed space formed by the seal cover, the inner packed column and the test tube is filled with the anaerobic microorganism culturing medium; and the anaerobic microorganism can be cultured and grow fast in the culturing medium and the culture condition is easy to control due to small sandwiched space, less residual air, and good nitrogen-blowing treatment and anaerobic effect. When a real monitoring is needed, the seal cover can be simply opened on site so as to insert the culturing medium for inoculation by a needle or an injector, and the operation in an anaerobic tank or an anaerobic glove box is no longer needed. The invention can be used for simply culturing and monitoring the anaerobic microorganism on site, reduces the errors of monitoring results and the time differences of test results, and has high detection rate of anaerobic bacteria.
Description
Technical field
The present invention relates to a kind of anaerobion culture apparatus, be specifically related to a kind of portable anaerobion culture apparatus.
Background technology:
Anaerobion is extensive in distributed in nature, and is of a great variety, and effect also draws attention day by day.The key problem in technology of cultivating anaerobion is that this quasi-microorganism is in the low environment of deoxidation or oxidation-reduction potential.The at present main principle that adopts anaerobism to cultivate has (1) chemical oxygen consumption, after pyrogallol and basic solution effect, forms alkaline gallate, can absorb oxygen and form anaerobic environment in this reaction process; Both contained unsaturated fatty acids in the beef slag and can absorb oxygen, containing gsh again, to form negative redox potential poor; (2) anaerobic jar is to adopt the oxygen of removing someway wherein, for example magnesium and zinc oxide are made the hydrogen producing bag, put into jar and add water reaction generation hydrogen, palladium or platinum are as catalyzer, catalysis Hydrogen and oxygen combine to form water at normal temperatures then can be removed the oxygen in the anaerobic jar of sealing.
The ordinary method of carrying out anaerobic bacteria culture in the laboratory comprises: Hungate rolling tube technique, anaerobic jar technology, anaerobism glove box technology.Also have substratum deoxygenation technology, Steel Wool deoxygenation technology, airbag anaerobic system and anaerobic petri diss technology etc. in addition.Because these anaerobism cultural methods and technical equipment conditional request height, the price comparison costliness operate comparatively loaded down with trivial detailsly, and common laboratory does not satisfy the requirements, and is not suitable for adopting the anaerobic jar cultivation for grass-roots unit; Fresh kitchen meat (beef slag) uses in basic unit also inconvenient; The operation of chemical oxygen consumption method such as pyrogallol is more loaded down with trivial details, and for big in batches, the sample more than batch is difficult to the control condition unanimity.Above-mentioned these several traditional microbial culture methods, being difficult in basic unit popularizes, often the anaerobion test item needs censorship, submitted sample ageing very poor again, at present more and more higher at the requirement of environment and Biosafety, be to realize that the anaerobism pathogenic micro-organism is on-the-spot timely and accurately to detect, just need exploitation a kind of portable, small-sized anaerobism testing tool not only makes the testing conditions unanimity, also needs to improve detection efficiency.Domestic at present, no matter be detection department or authoritative laboratory, all also using traditional anaerobic device to detect, the device that does not also have commercial simple and easy anaerobism to cultivate, the rarely seen report of the research and development of portable efficiently anaerobion culture apparatus.
Summary of the invention:
The purpose of this invention is to provide a kind of small-sized, easy portable anaerobion culture apparatus.
Portable anaerobion culture apparatus of the present invention, it is characterized in that, comprise the sealing cover of test tube and this test tube opening of sealing, also be provided with interior packed column, in the enclosed space that sealing cover, interior packed column and test tube three form, fill full anaerobion substratum in the inside of test tube.
Described test tube is preferably transparent glass test tube, is convenient to observe cultivation results.
In order to save substratum, in the enclosed space that sealing cover, interior packed column and test tube three form, close inboard wall of test tube and sealing cover that the outer wall of interior packed column should be tried one's best, an end of the close test tube opening end of therefore described interior packed column is preferably a little less than the test tube opening.
The preferred bore of described test tube is 13mm, and height is 100mm, and described interior packed column is preferably the cylindrical tube that both ends open is closed, and its bore is 9mm, and height is 95mm.
Described anaerobion substratum is a substratum of cultivating anaerobion, the kind of anaerobion substratum as well known to those skilled in the art and component thereof, anaerobion in this anaerobion substratum in the culture sample send professional institution to do further detection then.Further for convenience of some common enteron aisle anerobes of on-the-spot Preliminary detection, can be according to the Physiology and biochemistry character of these bacteriums, in different portable anaerobion culture apparatuses, fill the selection substratum of differentiating usefulness, according to the positive and the negative reaction of these substratum, tentatively judge detected result.Need in anaerobic jar or in the anaerobism glove box, operate than of the prior art, the physiological and biochemical property of this bacterium of mensuration one by one, portable anaerobion culture apparatus of the present invention, need not in anaerobic jar or anaerobism glove box, to operate, only need directly sample to be inoculated into fast at the scene in the anaerobion substratum in the portable anaerobion culture apparatus, disposable, the very easy identification experiment of finishing, Preliminary detection goes out the kind of anaerobion.Certainly, this culture can be sent professional feeler mechanism to detect in order further to examine.
Using method of the present invention is such, according to the requirement of portable anaerobion culture apparatus of the present invention, and with test tube, sealing cover and the sterilization of interior packed column, configuration anaerobion substratum, and it is stand-by to sterilize.Under aseptic condition, interior packed column is placed test tube, perfusion anaerobion substratum in the interlayer space between interior packed column and test tube, nitrogen flushing is caught up with oxygen, is avoided residual bubble in the filling process, after filling substratum in interlayer, seal up the test tube mouth with sealing cover, make interior filling of enclosed space of sealing cover, interior packed column and test tube expire the anaerobion substratum.During use, turn on sealing cover, adopt acupuncture (aseptic bamboo let) or syringe that sample is inserted the anaerobion inoculation of medium, cover sealing cover, place proper temperature to cultivate, generally through about 24 hours, the anaerobion substratum in the tube wall gap promptly has metachromasia, can tentatively judge detected result.
With the triple sugariron substratum as the anaerobion substratum in the portable anaerobion culture apparatus of the present invention, place common incubator and anaerobic jar to cultivate respectively then with the bacterium in this portable anaerobion culture apparatus cultivation table 1, and with this device.As substratum, with the bacterium in the deep layer slant culture table 1, and place common incubator and anaerobic jar to cultivate respectively, in contrast with the triple sugariron substratum.Experimental result is as shown in table 1.
Table 1 anaerobism culture effect controlled trial table
Annotate: the common incubator of 1, aerobic----; Anaerobism----is cultivated in the anaerobic jar; 2 ,+variable color (red stain Huang, fermentation, growth); ++ variable color (the red stain Huang ferments, growth); +++variable color (red stain Huang, it is fine to ferment, growth); * blackening (sulfide being arranged, growth) 3, large intestine are represented intestinal bacteria, and the sramana represents Salmonellas, secondary molten vice hemolysis vibrion, meat poison expression Clostridium botulinum.
Intestinal bacteria and Salmonellas belong to facultative anaerobe, and the culture effect in common incubator is little with the anaerobic jar difference basically, and the anaerobism of Vibrio parahaemolyticus and Clostridium botulinum requires to belong to strictly anaerobic bacterium than higher.
Adopt portable anaerobion culture apparatus of the present invention to cultivate above-mentioned bacterium, the growth result in common incubator is consistent basically with the effect of anaerobic jar; Traditional deep layer slant culture, not long basically common incubator (under the aerobic conditions) lining Vibrio parahaemolyticus and Clostridium botulinum, in anaerobic jar, Vibrio parahaemolyticus and Clostridium botulinum that deep layer is cultivated could be grown, show thus, portable anaerobion culture apparatus anaerobism of the present invention is effective, can promptly cultivate anerobe in the aerobic environment at common incubator.
The present invention is filled into the anaerobion substratum in the mezzanine space of test tube and interior packed column, and make interior filling of enclosed space of sealing cover, interior packed column and test tube expire the anaerobion substratum, because mezzanine space is little, residual air is few, almost can ignore, it is effective to add that the back anaerobism is handled in nitrogen flushing, anaerobion can be in this anaerobion substratum incubation growth, and fast growth, culture condition are controlled easily.When the monitoring of reality, only need the on-the-spot sealing cover of opening portable anaerobion culture apparatus of the present invention, acupuncture or syringe insert the anaerobion inoculation of medium with sample and get final product, need not in anaerobic jar or anaerobism glove box, to operate, if cleaner place, even the aseptic technique platform all need not, therefore cultivation that can be easy at the scene, monitoring anaerobion, reduced the time difference of the sum of errors detected result of monitoring result, anerobe recall rate height, this interlayer cultural method is effectively novel.
Portable anaerobion culture apparatus of the present invention not only can be used for the monitoring of aquaculture system, also can do the monitoring and the environmental ecology monitoring of anaerobism pathogenic micro-organism in medical microbial detection and food-processing and the storage process, applied widely, cost is low, has social benefit and market outlook widely.
Description of drawings:
Fig. 1 is the split figure of portable anaerobion culture apparatus of the present invention;
Fig. 2 is with the cylindrical tube invisible spectro synoptic diagram of packing into;
Fig. 3 is the overall schematic that portable anaerobion culture apparatus of the present invention assembles.
Wherein 1, transparent glass test tube; 2, cylindrical tube; 3, sealing cover; 4, anaerobion substratum;
Embodiment:
Below be to further specify to of the present invention, rather than limitation of the present invention.
One, the preparation of substratum:
Improvement M9 basic medium: adding sodium formiate to final concentration in the M9 substratum is that 10mmol/l and SODIUMNITRATE to final concentration are 1mmol/l.
1. improve M9+ glucose: add glucose in improvement M9 basic medium, making its quality volume fraction is 0.2% (w/v).
2. improve M9+ sucrose: add sucrose in improvement M9 basic medium, making its quality volume fraction is 0.2% (w/v).
3. improve the M9+ pectinose: add pectinose in improvement M9 basic medium, making its quality volume fraction is 0.2% (w/v).
4. improve the M9+ seminose: add seminose in improvement M9 basic medium, making its quality volume fraction is 0.2% (w/v).
5. improve M9+ fructose: add fructose in improvement M9 basic medium, making its quality volume fraction is 0.2% (w/v).
6. improve the M9+ lactose: add lactose in improvement M9 basic medium, making its quality volume fraction is 0.2% (w/v).
7. improve the M9+ inositol: add inositol in improvement M9 basic medium, making its quality volume fraction is 0.2% (w/v).
8. lactose bile salt medium: contain peptone 20.0g, bovine bile 5.0g, lactose 10.0g, agar 10.0g, purpurum bromocresolis 0.01g in every liter of substratum, surplus is a water, pH7.2～7.4.
9. triple sugariron substratum: contain the phenol red solution 10ml of peptone 20.0g, extractum carnis powder 5.0g, lactose 10.0g, sucrose 10.0g, glucose 1.0g, sodium-chlor 5.0g, ferrous sulfate 0.2g, Sulfothiorine 0.2g, agar 12.0g, 0.2g/100ml in every liter of substratum, surplus is a water.
10. peptone calcium chloride (60g./L) substratum: contain peptone 10.0g, sodium-chlor 60.0g, agar 10.0g, phenol red 0.2g in every liter of substratum, surplus is a water, pH7.2～7.4.
11. improvement MC substratum: contain soy peptone 5.0g, beef extract 5.0g, yeast powder 5.0g, glucose 20.0g, lactose 20.0g, lime carbonate 10.0g, toluylene red 0.05g, agar 10.0g in every liter of substratum, surplus is a water, pH7.2～7.4.
Two, the common typical enteropahtogenic microganism of Jian Ceing:
Intestinal bacteria Escherichia coli; Salmonellas Salmonella lignieres; Vibrio parahaemolyticus Vibrio parahaemolytics; Vibrio cholerae Vibrio choleras; Actinomyces pseudonecrophorus Fusobacterium necrophorum; Fusobacterium aquatile Fusobacterium aquatile; Bifidobacterium adolescentis Bifidobacteriun adolescentis;
Table 2: the Physiology and biochemistry character of common enteric microorganism (according to " the outstanding division bacteria handbook of uncle the 8th edition)
Annotate :+, long (corresponding metachromasia is arranged, represents with detecting 1 in the program); One, do not grow (no metachromasia is not represented with detecting 0 in the program);
D, different serotypes differential responses result (in program, representing) with uncertain 2.
Judge whether be the computer programming language that above-mentioned common typical enteropahtogenic microganism is used:
program?final；
const
n＝7；m＝11；
a:array[1..n，1..m]of?integer＝((1，1，1，1，2，1，0，1，0，0，0)，
(1，1，0，1，2，2，0，0，1，0，0)，
(0，0，0，0，2，2，0，0，1，1，0)，
(1，1，0，1，1，1，0，1，0，0，0)，
(1，0，0，0，0，0，0，0，0，0，0)，
(1，1，1，1，1，1，0，0，0，0，0)，
(1，1，0，0，1，1，0，0，0，0，1))；
quan:array[1..m]ofinteger＝(0，0，0，0，0，0，0，1，1，1，1)；
{(0，0，0，0，0，0，0)，
(0，0，0，0，0，0，0)，
(0，0，0，0，0，0，0)，
(0，0，0，0，0，0，0)，
(0，0，0，0，0，0，0)，
(0，0，0，0，0，0，0))；}
name:array[1..n]of?string＝(′E.coli′，′S.lignieres′，′V.parahaemolytics′，′V.choleras′，
，′F.necrophorum′，′F.aquatile′，′B.adolescentis′)；
var
i，j，k，degree:integer；
Ftang，Fid，Fposs，F4:BOOLEAN；
re:array[1..m]of?integer；
begin
writeln(′please?input?the?result:′)；
for?i:＝1?to?m?do
begin
read(re[i])；
end；
readln；
for?i:＝1?to?n?do
begin
ftang:＝true；
fid:＝true；
fposs:＝true；
for?j:＝1?to?m?do
begin
if(quan[j]＝1)and(a[i，j]＝1)then?fposs:＝false；
if?quan[j]＝0?then
begin
if(a[i，j]<>2)and(re[j]＜a[i，j])then?ftang:＝false；
end?else
begin
if(a[i，j]<>2)and(re[j]＜a[i，j])then?fid:＝false；
end；
end；
if?fid?then
begin
if?ftang?then
begin
if?fposs?then?writeln(′there?may?be′，name[i])
else
writeln(′there?must?be′，name[i])；
end
else
begin
if?fposs?then?writeln(′there?is?probably?not′，name[i])
else
writeln(′there?probably?is′，name[i])；
end；
end?else
begin
writeln(′there?cant?be′，name[i])；
end；
end；
end
Embodiment 1:
Shown in Fig. 1,2 and 3, the portable anaerobion culture apparatus of present embodiment, comprise transparent glass test tube 1, its bore is 13mm, height is 100mm, the sealing cover 3 that also has this transparent glass test tube opening of sealing, sealing cover 3 and transparent glass test tube 1 opening are by being threaded, also has the cylindrical tube 2 that both ends open is closed in the inside of transparent glass test tube 1, its bore is 9mm, height is 95mm, fills full anaerobion substratum 4 in the enclosed space that sealing cover 3, cylindrical tube 2 and transparent glass test tube 1 three form.
With transparent glass test tube 1, sealing cover 3 and cylindrical tube 2 sterilizations, stand-by.Under aseptic condition, cylindrical tube 2 is placed transparent glass test tube 1,11 kinds of substratum (anaerobion substratum) in the interlayer space between cylindrical tube 2 and transparent glass test tube 1 in the above-mentioned table 2 of perfusion, different substratum is poured into different portable anaerobion culture apparatuses respectively, oxygen is caught up with in nitrogen flushing in the filling process, avoid residual bubble, after filling substratum in interlayer, seal up the test tube mouth with sealing cover, and make at sealing cover 3, fill full anaerobion substratum 4 in the enclosed space that cylindrical tube 2 and transparent glass test tube 1 three form, and according to the serial number of the substratum in the table 2.
During use, turn on sealing cover, adopt acupuncture (aseptic bamboo let) or syringe that sample is inserted the anaerobion inoculation of medium, cover sealing cover, place proper temperature to cultivate, generally through about 24 hours, the anaerobion substratum in the tube wall gap promptly has metachromasia, can tentatively judge detected result.
(1) with Salmonellas S.lignieres, adopt acupuncture (aseptic bamboo let) to be inserted in the substratum, place 37 ℃ of temperature to cultivate, the anaerobic bacteria culture base about 24 hours in the tube wall gap promptly has metachromasia, and concrete outcome sees table 3.
Table 3: Salmonellas S.lignieres is through the portable anaerobion culture apparatus detected result of present embodiment
| Numbering | ?1? | 2? | 3? | 4? | 5? | 6? | 7? | 8? | 9? | 10? | 11? |
| The result | ?+? | +? | -? | +? | +? | -? | -? | -? | +? | -? | -? |
Annotate: the substratum in the different portable anaerobion culture apparatuses of numbering representative, wherein 1, improvement M9+ glucose; 2, improvement M9+ sucrose; 3, improvement M9+ pectinose; 4, improvement M9+ seminose; 5, improvement M9+ fructose; 6, improvement M9+ lactose; 7, improvement M9+ inositol; 8, lactose cholate; 9, triple sugariron; 10, peptone calcium chloride (60g/L); 11, improvement MC (corresponding programming form)
The program judged result:
With the experimental observation result in the above table according to :+, long (corresponding metachromasia is arranged, represents with detecting 1 in the program);
One, do not grow (no metachromasia is not represented with detecting 0 in the program); D, different serotypes differential responses result (representing with uncertain 2 in program) insert program, operation:
The result judges: Salmonellas S.lignieres detects correct.
(2) bifidobacterium adolescentis B.adolescentis
Adopt syringe that bifidobacterium adolescentis B.adolescentis is inserted anaerobion inoculation of medium in above-mentioned 11 portable anaerobion devices that contain above-mentioned 11 kinds of substratum, place 37 ℃ of temperature to cultivate, anaerobic bacteria culture base about 24 hours in the tube wall gap promptly has metachromasia, specifically see table 4, can tentatively judge detected result.
Table 4 bifidobacterium adolescentis B.adolescentis is through the portable anaerobion culture apparatus detected result of present embodiment
| Numbering | ?1? | 2? | 3? | 4? | 5? | 6? | 7? | 8? | 9? | 10? | 11? |
| The result | ?+? | +? | -? | -? | +? | +? | -? | -? | -? | -? | +? |
Annotate: the substratum in the different portable anaerobion culture apparatuses of numbering representative, wherein 1, improvement M9+ glucose; 2, improvement M9+ sucrose; 3, improvement M9+ pectinose; 4, improvement M9+ seminose; 5, improvement M9+ fructose; 6, improvement M9+ lactose; 7, improvement M9+ inositol; 8, lactose cholate; 9, triple sugariron; 10, peptone calcium chloride (60g/L); 11, improvement MC (corresponding programming form)
The program judged result:
With the experimental observation result in the above table according to :+, long (corresponding metachromasia is arranged, represents with detecting 1 in the program);
One, do not grow (no metachromasia is not represented with detecting 0 in the program); D, different serotypes differential responses result (representing with uncertain 2 in program) insert program, operation:
The result judges: bifidobacterium adolescentis B.adolescentis detects correct.
(3): the mixed bacteria liquid of intestinal bacteria, Salmonellas, Vibrio parahaemolyticus, bifidobacterium adolescentis.
Adopt acupuncture (aseptic bamboo let) that above-mentioned mixed bacteria liquid is inserted anaerobion inoculation of medium in above-mentioned 11 portable anaerobion devices that contain above-mentioned 11 kinds of substratum, place 37 ℃ of temperature to cultivate, substratum about 24 hours in the tube wall gap promptly has metachromasia, concrete outcome is as shown in table 5, can tentatively judge detected result.
Table 5 mixed bacteria liquid is through the portable anaerobion culture apparatus detected result of present embodiment
| Numbering | ?1? | 2? | 3? | 4? | 5? | 6? | 7? | 8? | 9? | 10? | 11? |
| The result | ?+? | +? | +? | +? | +? | +? | -? | +? | +? | +? | +? |
Annotate: the substratum in the different portable anaerobion culture apparatuses of numbering representative, wherein 1, improvement M9+ glucose; 2, improvement M9+ sucrose; 3, improvement M9+ pectinose; 4, improvement M9+ seminose; 5, improvement M9+ fructose; 6, improvement M9+ lactose; 7, improvement M9+ inositol; 8, lactose cholate; 9, triple sugariron; 10, peptone calcium chloride (60g/L); 11, improvement MC (corresponding programming form)
The program judged result:
With the experimental observation result in the above table according to :+, long (corresponding metachromasia is arranged, represents with detecting 1 in the program);
One, do not grow (no metachromasia is not represented with detecting 0 in the program); D, different serotypes differential responses result (representing with uncertain 2 in program) insert program, operation:
The result judges: intestinal bacteria Salmonellas Vibrio parahaemolyticus bifidobacterium adolescentis detects correct.
Show by the foregoing description, portable anaerobion culture apparatus anaerobism of the present invention is effective, detector efficiency can be effectively improved, and substratum substratum can be provided with according to the physiological and biochemical properties of some common intestinal bacteria, thus can preliminary evaluation anerobe.
Claims (5)
1. portable anaerobion culture apparatus, it is characterized in that, comprise the sealing cover of test tube and this test tube opening of sealing, also be provided with interior packed column, in the enclosed space that sealing cover, interior packed column and test tube three form, fill full anaerobion substratum in the inside of test tube.
2. portable anaerobion culture apparatus according to claim 1 is characterized in that described test tube is transparent glass test tube.
3. portable anaerobion culture apparatus according to claim 1 is characterized in that, an end of the close test tube opening end of described interior packed column is a little less than the test tube opening.
4. portable anaerobion culture apparatus according to claim 1 is characterized in that, the selection substratum of described anaerobion substratum for differentiating that anerobe is used.
5. portable anaerobion culture apparatus according to claim 1 is characterized in that described test tube bore is 13mm, and height is 100mm, and described interior packed column is the cylindrical tube that both ends open is closed, and its bore is 9mm, and height is 95mm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102925615A CN102021113A (en) | 2010-09-21 | 2010-09-21 | Portable anaerobic microorganism culturing device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102925615A CN102021113A (en) | 2010-09-21 | 2010-09-21 | Portable anaerobic microorganism culturing device |
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| CN2010102925615A Pending CN102021113A (en) | 2010-09-21 | 2010-09-21 | Portable anaerobic microorganism culturing device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109706055A (en) * | 2019-01-04 | 2019-05-03 | 南通科技职业学院 | A kind of anaerobe incubator |
| CN111057645A (en) * | 2019-12-31 | 2020-04-24 | 百沃星联(上海)环保科技有限公司 | Microbial storage device for food decomposition and storage method thereof |
| CN111269821A (en) * | 2020-03-24 | 2020-06-12 | 宁波大学 | Microorganism culture device and method |
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| US4140489A (en) * | 1977-02-07 | 1979-02-20 | Lee Sun Y | Test tube for easy enumeration and cultivation of anaerobic and facultatively anaerobic microorganisms |
| CN2237048Y (en) * | 1995-12-22 | 1996-10-09 | 刘德鹏 | Disposable end product biochemical tube |
| CN2528777Y (en) * | 2001-09-25 | 2003-01-01 | 张舜 | Horizontal multifunctional microbial reactor |
| CN101402989A (en) * | 2006-12-04 | 2009-04-08 | 株式会社安泰科拓 | Syringe-shaped microorganism culture device |
| CN201301313Y (en) * | 2008-11-29 | 2009-09-02 | 宁波大学 | Photosynthetic bacteria culturing device |
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| US4140489A (en) * | 1977-02-07 | 1979-02-20 | Lee Sun Y | Test tube for easy enumeration and cultivation of anaerobic and facultatively anaerobic microorganisms |
| CN2237048Y (en) * | 1995-12-22 | 1996-10-09 | 刘德鹏 | Disposable end product biochemical tube |
| CN2528777Y (en) * | 2001-09-25 | 2003-01-01 | 张舜 | Horizontal multifunctional microbial reactor |
| CN101402989A (en) * | 2006-12-04 | 2009-04-08 | 株式会社安泰科拓 | Syringe-shaped microorganism culture device |
| CN201301313Y (en) * | 2008-11-29 | 2009-09-02 | 宁波大学 | Photosynthetic bacteria culturing device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109706055A (en) * | 2019-01-04 | 2019-05-03 | 南通科技职业学院 | A kind of anaerobe incubator |
| CN111057645A (en) * | 2019-12-31 | 2020-04-24 | 百沃星联(上海)环保科技有限公司 | Microbial storage device for food decomposition and storage method thereof |
| CN111269821A (en) * | 2020-03-24 | 2020-06-12 | 宁波大学 | Microorganism culture device and method |
| CN111269821B (en) * | 2020-03-24 | 2023-12-19 | 宁波大学 | Microorganism culture device and method |
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