SU800192A1 - Anaerobic cultivator - Google Patents

Description

(54) ANAEROBIC CULTIVATOR

The invention relates to microbiology, in particular to the work with aaaerobic microorganisms. Closest to the proposed one is a cultivator that contained a kraika tank, vessels with microorganisms placed in it and a gas supply nozzle l. Its disadvantages include the fact that it is massive and inconvenient in operation, since it accommodates few cultivation vessels. Gas is replaced in it by chemical absorption or vacuuming, which leads to rapid corrosion of metal parts and wear to the apparatus. With a large amount of work, many such cultivators are required, which is not always possible due to their high cost and bulkiness. The purpose of the invention is to simplify the design. The goal is achieved by the fact that the neck of the container has a transverse groove for fastening the lid in it, and at least one longitudinal groove for the gas to escape, while the lid is made of an elastic, gas-tight material. FIG. 1 shows an anaerobic cultivator, general view; FIG. 2 is a section A-A of FIG. one; on fng. 3 - the neck of the anaerobic cultivator; in fig. 4 - longitudinal groove in the closed state; in fig. 5 - longitudinal groove in the opened state; on f. 6, section B-B in FIG. five; in fig. 7 cover. The anaerobic cultivator consists of an eiiflcocTH 1 and an elastic gas-tight cover 2. It has a neck 3 and a branch pipe 4. The shaft 2 is fixed in the transverse groove 5 of the neck 3 with a rubber compression ring 6. The branch pipe 4 of the rubber plug has a plug 7 through which the flowline has passed through a section of the flow pattern and passed through the wall. tube “” of a rubber tube 9, a case for introducing gas, (for sealing) with glass plow 10. Petri dishes 11 with microorganemia crops are placed on a stand 12. Into anaerobic cultivator insert a test tube 13 with a chemical indicator oxygen but. The anaerobic cultivator is provided with cylindrical glass vessels 14, into which oxygen absorbers can be placed. In the case of cylindrical vessels 14, a carbon dioxide generator 15 is mounted, for which it has a psmänsch: ipo6; ipKa 6 with a height of 7-10 cm,

The container 1 may be made of glass, plexiglass, or some other gas-tight material. If the container 1 is made of elastic plastic, then its neck 3 should be strengthened on the inner side with a metal or plastic ring 17,

The neck 3 in the upper part (with a vertical plane section) IMEBT has the shape of a circle 18, which passes B below. The thinner wall of the tank 1 ,, and the thickness reduction occurs on the outside of the neck, which contributes to the formation of a transverse groove 5 on the neck, the surface, at the level across the groove 5, the neck 3 has a longitudinal groove 19, which, together with the adjacent cap 2, performs the role of a safety valve.

Krnacka 2 has a flat upper part 20, vertical side walls 21 and horizontal floors 22.

Stand 12 is made of two equal halves.

Anaerobic cultivator works as follows.

Container 1 is sown with crops 11, a body of oxygen that can be placed in cylinders 14 or directly to the bottom of tank 1, tube 13 with an oxygen indicator and carbon dioxide generator 15. Capacity 1 is closed with a cap 2, the latter is secured with a ring b, and air is displaced from an anaerobic cultivator by blowing a harmless way — they grow al microbes with gas (nitrogen, hydrogen, propane, etc.). through the tube 9. The displaced air under the pressure of the injected gas is drawn out through the longitudinal groove 19 under the cover 2. The cover 2 is inflated under the influence of increasing pressure, its wall above the neck 3 is lifted and the entrance to the longitudinal groove D9 is opened. through which excess gas escapes (the gas path is indicated by the arrow). After reducing the pressure, the longitudinal groove 19 is closed again and the system becomes sealed.

The carbon dioxide generator 15 is assembled immediately before it is introduced into the anaerobic cultivator. For this, several pieces of marble are put into cylinder 14, water is poured in and then test tube 16 filled with concentrated hydrochloric acid is inserted. The water level should be higher than the top edge of the tube 16. Such a system ensures the early release of carbon dioxide and maintains an increased pressure in the anaerobic cultivator, which prevents the penetration of acid-like air into the cultivator through possible looseness in the joints for several days.

The anaz prepared in this way;: An obsolete cultivator is placed in a conventional thermostat for growing a crop.

The proposed device can be three models ;: 36, 10 and 6 Petri dishes. The latter model is designed to work with anaerobic boxes and does not have a tube. In addition to cultivating anaerobes, the device can be used to store anaerobic cultures in museums, as well as to store pre-prepared anaerobic culture media. A lysobic cultivator can also be used to germinate microbes that require a special gaseous medium for their growth, for example, brucella, which are used for their growth in carbon dioxide.

Closing an anaerobic cultivator does not require large expenditures and costs less than the cost of currently used devices with a similar purpose. The use of glass or plastics for the manufacture of the proposed device makes it even more economically 3 compared to metal devices:; which are easily and quickly subjected to corrosion, are difficult to clean and are therefore unhygienic in operation. The simplicity of the design makes the anaerobic cultivator convenient for handling, allowing it to be easily prepared for work and quickly completed with crops. The use of the proposed device will allow mass studies of anaerobic microorganisms in virtually any conditions.

Claims (1)

1. Microaerostat MI. Model 752, Price List No. 28-02-22. The Ministries of Medical Industry of the USSR Headquarters Medical Equipment and the Leningrad Order of Lenin and the Order of the October Revolution of the Revolution Production Association Krasnogvardeets, 1972. Xy VififXlpfV XXW i. r A If ) -h) and 7 / i , tf