CN106718031A - A kind of Cordyceps militaris hot weather cultivating technique - Google Patents

A kind of Cordyceps militaris hot weather cultivating technique Download PDF

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Publication number
CN106718031A
CN106718031A CN201611077519.5A CN201611077519A CN106718031A CN 106718031 A CN106718031 A CN 106718031A CN 201611077519 A CN201611077519 A CN 201611077519A CN 106718031 A CN106718031 A CN 106718031A
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China
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temperature
pure water
cordyceps militaris
round pool
hot weather
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CN201611077519.5A
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沙叶涛
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Zhangjiagang Union Biology Research Institute Co Ltd
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Zhangjiagang Union Biology Research Institute Co Ltd
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Priority to CN201611077519.5A priority Critical patent/CN106718031A/en
Publication of CN106718031A publication Critical patent/CN106718031A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of Cordyceps militaris hot weather cultivating technique, comprise the following steps:Sterilizing, inoculation, bacteria developing period culture, cultural hypha, fructification culture and results, round pool is set up in culturing room, pure water is provided with round pool and so that several vial culture mediums are swum on the water surface, pure water is provided with round pool and so that several vial culture mediums are swum on the water surface, the side of round pool is provided with refrigeration machine and pure water is lowered the temperature, and the temperature of vial culture medium is reduced using the low temperature of pure water.Through the above way, Cordyceps militaris hot weather cultivating technique of the present invention, is lowered the temperature using refrigeration machine to pure water, and the temperature by pure water carries out temperature control to vial culture medium, temperature controlled high precision, energy consumption is low, be conducive to the growth of Cordyceps militaris, improve yield rate.

Description

A kind of Cordyceps militaris hot weather cultivating technique
Technical field
The present invention relates to Cordyceps militaris field, more particularly to a kind of Cordyceps militaris hot weather cultivating technique.
Background technology
Cordyceps militaris is a kind of sac fungus, and zoogamy is carried out by heterothallism.Its phorozoon draws up mould for pupa.Its son Ascospore can be formed after entity maturation(Reproduction unit), spore propagates with the wind after distributing, and spore falls on suitable polypide, just Start sprouting and form mycelium.Mycelium one side is constantly developed, and simultaneously starts to be spread in polypide, and then pupa worm will be true Bacterium infects, and decomposes the tissue in pupal cell, using the matter and energy source that the nutrition in pupal cell is grown as it, finally by pupa Internal portion decomposes completely.
Natural Cordyceps militaris yields poorly, complicated component, and the Cordyceps militaris yield of artificial culture is high, and composition is relatively pure.But It is, the summer high temperature weather higher to temperature control requirement during cultivation, the individual growth of influence Cordyceps militaris, and it is sharp Energy consumption with air-conditioning reduction indoor temperature is big, and reaction is slow, and low precision is, it is necessary to modified technique.
The content of the invention
The present invention solves the technical problem of a kind of Cordyceps militaris hot weather cultivating technique is provided, reduce and accurately control The temperature of vial culture medium processed, the yield that lifting hot weather Cordyceps militaris is cultivated.
In order to solve the above technical problems, one aspect of the present invention is:A kind of Cordyceps militaris hot weather is provided Cultivating technique, comprises the following steps:
Sterilizing:Vial culture medium is carried out into sterilization, it is small that vial culture medium is placed on 110 DEG C of high-temperature sterilizations half in pressure cooker When, taken out after then dropping to room temperature, move in transfer room;
Inoculation:To living silkworm chrysalises are put into vial culture medium, liquid spawn is injected, then seal bottle with transparent plastic sheeting Culturing room is moved into after mouthful;
Bacteria developing period culture:Round pool is set up in culturing room, pure water is provided with round pool and several glass are caused Bottle culture medium is swum on the water surface, and the side of round pool is provided with refrigeration machine and pure water is lowered the temperature, at the hair bacterium initial stage Keep dark in culturing room and avoid ventilation, relative air humidity remains 65%, control the temperature of pure water for 18 DEG C, wait to expect Face rises high-temperature to 21 DEG C after covering with mycelia;
Cultural hypha:Cultural hypha early stage is 1 ~ 2 day, and the temperature control of early stage pure water on daytime is 22 DEG C, and night temperatures are 9 DEG C, relative air humidity 70% carries out illumination using 40 watts of incandescent lamps, and light application time is 10 hours;
The cultural hypha later stage is 2 ~ 5 days, and daytime, the temperature control of pure water was 25 DEG C, and night temperatures are 10 DEG C, and air is relatively wet Degree is maintained at 80%, and incandescent lamp using 45 watts carries out illumination, and light application time is 16 hours, and mycelia gradually becomes tangerine by white Yellow, switch to be further cultured for after crocus completely to enter for 2 days 2 days it is out of grass, the temperature of pure water is maintained at 25 DEG C, and air is relatively warm Degree 90%, 45 watts of incandescent lamp carries out the illumination of daily 16 hours;
Fructification culture:5 apertures are pierced on a plastic film, and the temperature control of pure water is in 20 DEG C, air phase in round pool 85% is maintained to humidity, 50 watts of incandescent lamp carries out the illumination of daily 16 hours, treats that fructification head starts to expand and no longer becomes When long, the illumination of daily 18 hours is changed to, pure coolant-temperature gage is kept for 24 DEG C, and relative air humidity keeps 90%;
Harvest:After fructification head expand holding it is constant after, be further cultured for 3 ~ 5 days so that apical head produce ascospore when, table Show that Cordyceps militaris is ripe, remove culturing room, take out Cordyceps militaris.
In a preferred embodiment of the present invention, it is provided with electronic impeller in the round pool and drives the current to carry out heat Exchange.
In a preferred embodiment of the present invention, the water inlet and delivery port of the refrigeration machine be respectively arranged with pipeline and with The both sides of round pool are connected, and the water inlet of the refrigeration machine is additionally provided with water pump.
In a preferred embodiment of the present invention, the wallboard of the round pool is clear glass or acrylic board.
In a preferred embodiment of the present invention, the electronics temperature with refrigeration machine linearly connected is provided with the round pool Degree meter.
The beneficial effects of the invention are as follows:A kind of Cordyceps militaris hot weather cultivating technique that the present invention is pointed out, using refrigeration machine Pure water is lowered the temperature, and temperature by pure water carries out temperature control, temperature controlled precision to vial culture medium Height, energy consumption is low, is conducive to the growth of Cordyceps militaris, improves yield rate, reduces the cost of production.
Specific embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation Example is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, this area is common All other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model of present invention protection Enclose.
The embodiment of the present invention includes:
A kind of Cordyceps militaris hot weather cultivating technique, comprises the following steps:
Sterilizing:Vial culture medium is carried out into sterilization, it is small that vial culture medium is placed on 110 DEG C of high-temperature sterilizations half in pressure cooker When, taken out after then dropping to room temperature, move in transfer room;
Inoculation:To living silkworm chrysalises are put into vial culture medium, liquid spawn is injected, then seal bottle with transparent plastic sheeting Culturing room is moved into after mouthful;
Bacteria developing period culture:Round pool is set up in culturing room, pure water is provided with round pool and several glass are caused Bottle culture medium is swum on the water surface, and the side of round pool is provided with refrigeration machine and pure water is lowered the temperature, the circular water Electronic impeller is provided with pond and drives the current to carry out heat exchange so that the temperature of pure water is more uniform, it is to avoid hot-spot Problem;
Keep dark in culturing room at the hair bacterium initial stage and avoid ventilation, relative air humidity remains 65%, controls the temperature of pure water It is 18 DEG C to spend, and high-temperature is risen after charge level covers with mycelia to 21 DEG C;
Cultural hypha:Cultural hypha early stage is 1 ~ 2 day, and the temperature control of early stage pure water on daytime is 22 DEG C, and night temperatures are 9 DEG C, relative air humidity 70% carries out illumination using 40 watts of incandescent lamps, and light application time is 10 hours, in the water inlet of refrigeration machine Pipeline being respectively arranged with delivery port and being connected with the both sides of round pool, the water inlet of the refrigeration machine is additionally provided with water Pump so that pure water enters refrigeration machine, in feeding round pool after cooling, recycles, low cost;
The cultural hypha later stage is 2 ~ 5 days, and daytime, the temperature control of pure water was 25 DEG C, and night temperatures are 10 DEG C, and air is relatively wet Degree is maintained at 80%, and incandescent lamp using 45 watts carries out illumination, and light application time is 16 hours, and mycelia gradually becomes tangerine by white Yellow, switch to be further cultured for after crocus completely to enter for 2 days 2 days it is out of grass, the temperature of pure water is maintained at 25 DEG C, and air is relatively warm Degree 90%, 45 watts of incandescent lamp carries out the illumination of daily 16 hours, and the wallboard of the round pool is clear glass or acrylic Plate, translucency is good, facilitates the use of illuminating lamp, is conducive to mycelia to receive illumination;
Fructification culture:5 apertures are pierced on a plastic film, and the temperature control of pure water is in 20 DEG C, air phase in round pool 85% is maintained to humidity, 50 watts of incandescent lamp carries out the illumination of daily 16 hours, treats that fructification head starts to expand and no longer becomes When long, the illumination of daily 18 hours is changed to, pure coolant-temperature gage is kept for 24 DEG C, and relative air humidity keeps 90%, the round pool The electronic thermometer with refrigeration machine linearly connected is inside provided with, facilitates the work of refrigeration machine, the temperature of precise control pure water;
Harvest:After fructification head expand holding it is constant after, be further cultured for 3 ~ 5 days so that apical head produce ascospore when, table Show that Cordyceps militaris is ripe, remove culturing room, take out Cordyceps militaris.
In sum, a kind of Cordyceps militaris hot weather cultivating technique that the present invention is pointed out, is adapted to make in summer high temperature weather With, the energy consumption of air-conditioning is reduced, the temperature of precise control vial culture medium coordinates the growth of Cordyceps militaris, lifts yield and finished product Rate.
Embodiments of the invention are the foregoing is only, the scope of the claims of the invention is not thereby limited, it is every to utilize this hair Equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks Domain, is included within the scope of the present invention.

Claims (5)

1. a kind of Cordyceps militaris hot weather cultivating technique, it is characterised in that comprise the following steps:
Sterilizing:Vial culture medium is carried out into sterilization, it is small that vial culture medium is placed on 110 DEG C of high-temperature sterilizations half in pressure cooker When, taken out after then dropping to room temperature, move in transfer room;
Inoculation:To living silkworm chrysalises are put into vial culture medium, liquid spawn is injected, then seal bottle with transparent plastic sheeting Culturing room is moved into after mouthful;
Bacteria developing period culture:Round pool is set up in culturing room, pure water is provided with round pool and several glass are caused Bottle culture medium is swum on the water surface, and the side of round pool is provided with refrigeration machine and pure water is lowered the temperature, at the hair bacterium initial stage Keep dark in culturing room and avoid ventilation, relative air humidity remains 65%, control the temperature of pure water for 18 DEG C, wait to expect Face rises high-temperature to 21 DEG C after covering with mycelia;
Cultural hypha:Cultural hypha early stage is 1 ~ 2 day, and the temperature control of early stage pure water on daytime is 22 DEG C, and night temperatures are 9 DEG C, relative air humidity 70% carries out illumination using 40 watts of incandescent lamps, and light application time is 10 hours;
The cultural hypha later stage is 2 ~ 5 days, and daytime, the temperature control of pure water was 25 DEG C, and night temperatures are 10 DEG C, and air is relatively wet Degree is maintained at 80%, and incandescent lamp using 45 watts carries out illumination, and light application time is 16 hours, and mycelia gradually becomes tangerine by white Yellow, switch to be further cultured for after crocus completely to enter for 2 days 2 days it is out of grass, the temperature of pure water is maintained at 25 DEG C, and air is relatively warm Degree 90%, 45 watts of incandescent lamp carries out the illumination of daily 16 hours;
Fructification culture:5 apertures are pierced on a plastic film, and the temperature control of pure water is in 20 DEG C, air phase in round pool 85% is maintained to humidity, 50 watts of incandescent lamp carries out the illumination of daily 16 hours, treats that fructification head starts to expand and no longer becomes When long, the illumination of daily 18 hours is changed to, pure coolant-temperature gage is kept for 24 DEG C, and relative air humidity keeps 90%;
Harvest:After fructification head expand holding it is constant after, be further cultured for 3 ~ 5 days so that apical head produce ascospore when, table Show that Cordyceps militaris is ripe, remove culturing room, take out Cordyceps militaris.
2. Cordyceps militaris hot weather cultivating technique according to claim 1, it is characterised in that set in the round pool There is electronic impeller and drive the current to carry out heat exchange.
3. Cordyceps militaris hot weather cultivating technique according to claim 1, it is characterised in that the water inlet of the refrigeration machine Pipeline being respectively arranged with delivery port and being connected with the both sides of round pool, the water inlet of the refrigeration machine is additionally provided with water Pump.
4. Cordyceps militaris hot weather cultivating technique according to claim 1, it is characterised in that the wallboard of the round pool It is clear glass or acrylic board.
5. Cordyceps militaris hot weather cultivating technique according to claim 1, it is characterised in that set in the round pool There is the electronic thermometer with refrigeration machine linearly connected.
CN201611077519.5A 2016-11-30 2016-11-30 A kind of Cordyceps militaris hot weather cultivating technique Pending CN106718031A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108925365A (en) * 2018-06-22 2018-12-04 江苏省农业科学院 A kind of Chinese caterpillar fungus culture medium and the Cordyceps militaris inoculation method based on the culture medium

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1271514A (en) * 2000-05-23 2000-11-01 曾树生 Process for preparing mushroom-shaped cordyceps mycelium
CN1650686A (en) * 2005-01-06 2005-08-10 江苏省农业科学院 Solid culturing method of north cateripillar fungus
CN1887049A (en) * 2005-06-28 2007-01-03 兰著碧 Aweto producing process
CN203708882U (en) * 2014-03-04 2014-07-16 云南金雨庄生物科技有限责任公司 Multifunctional liquid strain culturing device
CN104303820A (en) * 2014-09-09 2015-01-28 山东健方生物科技有限公司 Large-scale production method of living silkworm cordyceps militaris

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1271514A (en) * 2000-05-23 2000-11-01 曾树生 Process for preparing mushroom-shaped cordyceps mycelium
CN1650686A (en) * 2005-01-06 2005-08-10 江苏省农业科学院 Solid culturing method of north cateripillar fungus
CN1887049A (en) * 2005-06-28 2007-01-03 兰著碧 Aweto producing process
CN203708882U (en) * 2014-03-04 2014-07-16 云南金雨庄生物科技有限责任公司 Multifunctional liquid strain culturing device
CN104303820A (en) * 2014-09-09 2015-01-28 山东健方生物科技有限公司 Large-scale production method of living silkworm cordyceps militaris

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108925365A (en) * 2018-06-22 2018-12-04 江苏省农业科学院 A kind of Chinese caterpillar fungus culture medium and the Cordyceps militaris inoculation method based on the culture medium

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