CN110423690A - A kind of cell factory pipe-line system - Google Patents
A kind of cell factory pipe-line system Download PDFInfo
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- CN110423690A CN110423690A CN201910727689.0A CN201910727689A CN110423690A CN 110423690 A CN110423690 A CN 110423690A CN 201910727689 A CN201910727689 A CN 201910727689A CN 110423690 A CN110423690 A CN 110423690A
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- pipe
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- nutrient solution
- culture
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
Abstract
The invention discloses a kind of cell factory pipe-line systems, it is related to technical field of cell culture, it includes fluid reservoir and culture tank, culture tank is provided with several, and supply pipe is communicated between any culture tank and fluid reservoir, any supply pipe is provided with electric flow control valve close to the side of culture tank, any supply pipe is provided with feedback control flow sensor close to the side of culture tank in electric flow control valve, and feedback control flow sensor is electrically connected with corresponding electric flow control valve and controls electric flow control valve；In real work, fluid reservoir actually enters the speed of culture tank to the maximum feed speed of each supply pipe not less than nutrient solution.The present invention helps to ensure that fluid reservoir to the feed speed of each culture tank nutrient solution and the consistency of supply amount, and the advantages of help to ensure that the training quality of cell.
Description
Technical field
The present invention relates to technical field of cell culture, more particularly, to a kind of cell factory pipe-line system.
Background technique
Cell culture refers to simulated in vivo environment (sterile, preference temperature, pH value and certain nutritional condition etc.) in vitro,
Make cells survival, growth, breeding and a kind of method for maintaining primary structure and function.
Currently, being the same cell of mass production, generally requiring several culture tanks in the industrialized production to cell
Production is cultivated the cells simultaneously.In actual job, to guarantee to cultivate the consistency of cell in several culture tanks, often
Unified configuration is carried out to nutrient solution, and nutrient solution is placed in a total fluid reservoir；Then, pipeline point is passed through by fluid reservoir
The supplying nutrition liquid not into culture tank.
However, the pressure that is initially supplied to that fluid reservoir gives each pipeline nutrient solution is consistent, due to culture tank volume compared with
Greatly, part culture tank is often farther away apart from fluid reservoir, seeks so as to cause supplying to the farther away culture tank of partial distance fluid reservoir
The pressure of nutrient solution becomes smaller, i.e., becomes smaller to the speed of part culture tank supplying nutrition liquid, and staff is mesotrophic to culture tank
It fills into and is usually completed by way of timing, and then cause the supply amount to part culture tank nutrient solution less, seriously affect training
The consistency of cell is supported, to seriously reduce the training quality of part cell, there are places to be modified.
Summary of the invention
In view of the above technical problems, present invention aims at a kind of cell factory pipe-line system is proposed, by the first feedback
It controls flow sensor and controls the control of the first electric flow control valve, and then the flow velocity of nutrient solution in supply pipe is controlled automatically
System thus helps to ensure that fluid reservoir to the feed speed of each culture tank nutrient solution and the consistency of supply amount, and facilitates
Guarantee the training quality of cell.
Above-mentioned technical purpose of the invention has the technical scheme that
A kind of cell factory pipe-line system, including fluid reservoir and culture tank, the culture tank are provided with several, and any described
Supply pipe is communicated between culture tank and fluid reservoir, which is characterized in that any supply pipe is equal close to the side of culture tank
It is provided with electric flow control valve, any supply pipe is provided with instead in electric flow control valve close to the side of culture tank
Feedback control flow sensor, the feedback control flow sensor are electrically connected with corresponding electric flow control valve and control electronic
Flow control valve；In real work, fluid reservoir actually enters training not less than nutrient solution to the maximum feed speed of each supply pipe
Support the speed of tank.
By using above-mentioned technical proposal, in real work, supply pipe is measured to culture from feedback control flow sensor
The pressure of tank supplying nutrition liquid, and control electric flow control valve so that pressure from supply pipe to culture tank supplying nutrition liquid and
Flow velocity reaches standard value, and makes each supply pipe flow velocity of supplying nutrition liquid into culture tank consistent.In this way, have
Help guarantee fluid reservoir to the feed speed of each culture tank nutrient solution and the consistency of supply amount, and helps to ensure that cell
Training quality.
The present invention is further arranged to: filter membrane is provided in the culture tank, and the filter membrane is by culture tank points
It is divided into culture chamber and drain chamber, the lower wall of the drain chamber is provided with and communicated with drain pipe, is connected on the drain pipe
There is control valve.
By using above-mentioned technical proposal, in culture tank when Additional nutrient solution, staff can open control valve,
Culture tank is discharged in split-off nutrient solution in the middle part of culture tank, and stops cell to be discharged nutrient canal by filter membrane；Then, by new nutrition
Liquid is supplied through supply pipe into culture tank.In this way, it helps to ensure that the concentration of nutritional ingredient in nutrient solution, and helps
Operation is normally cultivated in guarantee cell.
The present invention is further arranged to: one end connection of the supply pipe far from culture tank is in culture chamber far from drain pipe
Side.
By using above-mentioned technical proposal, one end connection by supply pipe far from culture tank is in culture chamber far from drain pipe
Side, occur to reduce a large amount of nutritional ingredients in the case where drain chamber deposition, facilitate promoted cell culture to nutrition
The utilization rate of liquid.
The present invention is further arranged to: the control valve is the first solenoid valve, is provided with high liquid level in the culture tank and opens
It closes and low liquid level switch, the supply pipe is provided with second solenoid valve close to the side of culture tank, the high level switch and low
Both liquid-level switches are electrically connected with second solenoid valve, and the high level switch and low liquid level switch jointly control the second electricity
Magnet valve；The low liquid level switch is electrically connected with the first solenoid valve and controls the first solenoid valve；When needing to supplement in culture tank
When nutrient solution, staff opens the first solenoid valve, and culture tank is discharged in the part nutrient solution in culture tank；When being sought in culture tank
When nutrient solution liquid level reaches low liquid level switch contact, the first solenoid valve of low liquid level switch control is closed, the second control valve of control is opened,
At this point, culture tank stops discharge nutrient solution, supply pipe supplying nutrition liquid into culture tank；When nutrient solution liquid level reaches in culture tank
When high level switch contact, supply pipe stops the supplying nutrition liquid into culture tank.
By using above-mentioned technical proposal, high level switch, low liquid level switch, the first solenoid valve and second solenoid valve connection
It closes the discharge of nutrient solution in control culture tank and fills into, greatly facilitate staff to the discharge of nutrient solution in culture tank and fill into
Operation, and help to ensure that the consistency of the discharge rate He the amount of filling into of nutrient solution in culture tank, guarantee that cell is normal in culture tank
Growth.
The present invention is further arranged to: the Jar opening sealer of the fluid reservoir has closing cap, and the upper connection of the closing cap is set
It is equipped with snorkel, third solenoid valve is provided on the snorkel, the snorkel is provided with and communicated with far from one end of closing cap
Air filter.
By using above-mentioned technical proposal, when fluid reservoir fills into nutrient solution to culture tank, staff can open
One solenoid valve, so that with the decline of liquid level and ventilating in fluid reservoir, and by air filter in entrance fluid reservoir
Air is filtered, and helps to ensure that fluid reservoir normally supplies operation to culture tank nutrient solution, is reduced external impurities and is entered storage
The case where flow container pollution nutrient solution, occurs.
The present invention is further arranged to: the stirring dress for stirring nutrient solution in fluid reservoir is provided on the fluid reservoir
It sets.
By using above-mentioned technical proposal, nutrient solution in fluid reservoir is stirred by agitating device, helps to ensure that battalion
The uniformity of nutritional ingredient in nutrient solution, and help to ensure that nutrient solution fills into the consistency of nutritional ingredient in each culture tank.
The present invention is further arranged to: the agitating device includes air blower, the first connecting tube and the second connecting tube, described
Air blower, the first connecting tube and the second connecting tube are arranged at the outside of culture tank, one end of first connecting tube and air blast
The connection of machine bleeding point, the other end of first connecting tube is connected to the upside of fluid reservoir, and first communicating pipe and liquid storage
Tank connectivity part is higher than the height of nutrient solution in fluid reservoir；One end of second communicating pipe is connected to the blowing mouth of air blower, institute
The other end for stating for the second communicating pipe is connected to the downside of fluid reservoir；Also, the height of the air blower is higher than nutrition in fluid reservoir
The height of liquid.
By using above-mentioned technical proposal, in real work, the air on the upside of fluid reservoir is extracted by air blower, and by air
It is delivered to the downside of fluid reservoir, air-impingement nutrient solution, and gas floating agitation nutrient solution, to realize to nutrition in fluid reservoir
The stirring operation of liquid.
The present invention is further arranged to: the agitating device further includes stirring pipe group, and the stirring pipe group includes in several
Root is interconnected the branch pipe of setting, and the upper of the branch pipe is provided with and communicated with gas distribution pipe in several, and the stirring pipe group with
Second communicating pipe was connected to far from one end of air blower suction opening.
By using above-mentioned technical proposal, by several gas distribution pipes in stirring pipe group simultaneously to the nutrient solution in fluid reservoir
It is stirred, facilitates the uniformity for promoting nutritional ingredient in nutrient solution.
The present invention is further arranged to: the gas distribution pipe is located at the downside of branch pipe and is directed toward the bottom wall of fluid reservoir.
By using above-mentioned technical proposal, in real work, the gas through gas distribution pipe discharge will impact fluid reservoir first
Bottom wall, to reduce the case where generation of fluid reservoir bottom wall precipitates；Also, after nutrient solution is completely exhausted out in fluid reservoir, stir
Stirring pipe group can be discharged in the nutrient solution for mixing Guan Zuzhong under the blowing of air-flow, facilitate reduction nutrient solution and be retained in stirring pipe group
In situation occur, and help to facilitate staff to the cleaning operation of stirring pipe group.
The present invention is further arranged to: any supply pipe is provided with heater close to the side of culture tank.
It can be by heater to the nutrition Jing Guo supply pipe when being in cold snap by using above-mentioned technical proposal
Liquid is heated, and is reduced low temperature nutrient solution and is entered in culture tank the case where influencing cell growing environment generation, helps to ensure that thin
Born of the same parents normally grow operation in culture tank.
Compared with prior art, beneficial effects of the present invention are as follows:
(1) control of the first electric flow control valve is controlled by the first feedback control flow sensor, and then to seeking in supply pipe
The flow velocity of nutrient solution carries out automatically controlling, and thus helps to ensure that feed speed and supply of the fluid reservoir to each culture tank nutrient solution
The consistency of amount, and help to ensure that the training quality of cell；
(2) nutrition in culture tank is jointly controlled by high level switch, low liquid level switch, the first solenoid valve and second solenoid valve
It the discharge of liquid and fills into, greatly facilitate staff to the discharge of nutrient solution in culture tank and fills into operation, and help to ensure that
The consistency of the discharge rate He the amount of filling into of nutrient solution in culture tank guarantees that cell is normally grown in culture tank；
(3) air blower, the first connecting tube, the second connecting tube, stirring pipe group and gas distribution pipe are comprehensively utilized, is realized in fluid reservoir
The stirring operation of nutrient solution, greatly promotes the uniformity of nutritional ingredient in nutrient solution, and helps to ensure that fluid reservoir to each training
Support the consistency of tank supplying nutrition liquid nutritional ingredient.
Detailed description of the invention
Fig. 1 is the system diagram of one major embodiment cell factory pipe-line system structure of embodiment；
Fig. 2 is the schematic diagram of one major embodiment culture jar structure of embodiment；
Fig. 3 is the schematic diagram of embodiment one major embodiment the first solenoid valve and second solenoid valve control circuit；
Fig. 4 is the schematic diagram of one major embodiment liquid storage tank structure of embodiment；
Fig. 5 is the schematic diagram of two major embodiment liquid storage tank structure of embodiment.
Appended drawing reference: 1, fluid reservoir；2, culture tank；21, filter membrane；22, chamber is cultivated；23, drain chamber；24, drain
Pipe；25, the first solenoid valve；26, high level switch；27, low liquid level switchs；3, supply pipe；31, electric flow control valve；32, anti-
Feedback control flow sensor；33, second solenoid valve；34, heater；4, agitating device；41, air blower；42, the first connecting tube；
43, the second connecting tube；44, stirring pipe group；45, branch pipe；46, gas distribution pipe；5, the first circuit；51, air switch；6, the second electricity
Road；61, the self-locking module of relay；62, relay coil；63, relay normally open contact；7, supply pump；71, perfusion tube；8, it seals
Close lid；81, snorkel；82, third solenoid valve；83, air filter.
Specific embodiment
Below with reference to examples and drawings, the present invention is described in further detail, but embodiments of the present invention are not only
It is limited to this.
Embodiment:
Referring to Fig. 1 and Fig. 4, a kind of cell factory pipe-line system, including fluid reservoir 1 and culture tank 2, culture tank 2 are provided with several
It is a, and it is communicated with supply pipe 3 between any culture tank 2 and fluid reservoir 1, any supply pipe 3 is all provided with close to the side of culture tank 2
It is equipped with electric flow control valve 31, any supply pipe 3 is provided with instead in electric flow control valve 31 close to the side of culture tank 2
Feedback control flow sensor 32, feedback control flow sensor 32 are electrically connected with corresponding electric flow control valve 31 and control electricity
Dynamic flow control valve 31.Also, the agitating device 4 for stirring nutrient solution in fluid reservoir 1 is provided on fluid reservoir 1.Wherein, instead
Controller is electrically connected between feedback control flow sensor 32 and electric flow control valve 31.
In real work, the nutrient solution in fluid reservoir 1 is stirred by agitating device 4, guarantees nutrient solution in fluid reservoir 1
The uniformity of nutritional ingredient；Then, fluid reservoir 1 can also pass through liquid storage by drawing liquid pump to 2 supplying nutrition liquid of culture tank
To supplying nutrition liquid in culture tank 2, (air pressure i.e. in fluid reservoir 1 is higher than in culture tank 2 pressure difference between tank 1 and culture tank 2
Air pressure or fluid reservoir 1 in hydraulic pressure be higher than culture tank 2 in hydraulic pressure), and fluid reservoir 1 to each supply pipe 3 maximum supply
The speed of culture tank 2 is actually entered not less than nutrient solution to speed.When fluid reservoir 1 fills into nutrient solution to culture tank 2, by feeding back
It controls flow sensor 32 and measures flow of the supply pipe 3 to 2 supplying nutrition liquid of culture tank, and transmit a signal to controller, by
Controller controls electric flow control valve 31, so that supply pipe 3 reaches pressure from mark to 2 supplying nutrition liquid of culture tank and flow velocity
Quasi- value, helps to ensure that fluid reservoir 1 to the feed speed of each 2 nutrient solution of culture tank and the consistency of supply amount.Wherein,
Each culture tank 2 and corresponding supply pipe 3 are identical in the mounting means of the cell factory pipe-line system, now with wherein one group into
Row illustrates.
Referring to Fig. 1 and Fig. 2, filter membrane 21 is provided in culture tank 2, filter membrane 21 will be divided into culture chamber in culture tank 2
Room 22 and drain chamber 23, the lower wall of drain chamber 23 are provided with and communicated with drain pipe 24, and the first electricity is communicated on drain pipe 24
Magnet valve 25.Also, one end connection of the supply pipe 3 far from culture tank 2 is in culture side of the chamber 22 far from drain pipe 24.When to training
When supporting Additional nutrient solution in tank 2, staff can open the first solenoid valve 25, and 2 middle part split-off nutrient solution of culture tank is discharged and is trained
Tank 2 is supported, and stops cell to be discharged nutrient canal by filter membrane 21；Then, new nutrient solution is supplied through supply pipe 3 to culture tank
In 2, the concentration of nutritional ingredient in nutrient solution is helped to ensure that.
Referring to figs. 2 and 3, further, control valve is the first solenoid valve 25, is provided with high level switch in culture tank 2
26 and low liquid level switch 27, supply pipe 3 is provided with second solenoid valve 33 close to the side of culture tank 2, high level switch 26 and low
Both liquid-level switches 27 are electrically connected with second solenoid valve 33, and high level switch 26 and low liquid level switch 27 jointly control the
Two solenoid valves 33；Low liquid level switch 27 and the first solenoid valve 25 are electrically connected and control the first solenoid valve 25.When needs are to culture
In tank 2 when Additional nutrient solution, staff opens the first solenoid valve 25, and culture tank is discharged in the part nutrient solution in culture tank 2
2；When nutrient solution liquid level reaches 27 contact of low liquid level switch in culture tank 2, low liquid level switch 27 controls the first solenoid valve 25 and closes
It closes, control the opening of second solenoid valve 33, at this point, culture tank 2 stops discharge nutrient solution, supply pipe 3 supplies nutrition into culture tank 2
Liquid；When nutrient solution liquid level reaches 26 contact of high level switch in culture tank 2, the second control valve is closed, and supply pipe 3 stops to training
Support supplying nutrition liquid in tank 2；Later, staff can close feedback control flow sensor 32 and electric flow control valve
31。
Specifically, the control circuit for the first solenoid valve 25 is the first circuit 5, the first circuit 5 includes air switch 51.
In ladder circuit figure, the both ends of the first circuit 5 are connected respectively in left and right bus, the first solenoid valve 25 and air switch 51
It is connected in series, low liquid level switch 27 is in parallel with air switch 51.
Control circuit for second solenoid valve 33 is second circuit 6, and second circuit 6 includes the self-locking module 61 of relay.
The self-locking module 61 of relay includes relay coil 62, relay normally open contact 63, starts switch and shutdown switch.Trapezoidal
It in circuit diagram, starts switch, shutdown switch and relay coil 62 and be electrically connected in series, the both ends difference of the circuit
It is correspondingly connected in left and right bus, relay normally open contact 63 is arranged in parallel with starting switch；Also, second solenoid valve 33 and relay
Device normally opened contact 63 is connected.Wherein, low liquid level switch 27 is to start switch, and high level switch 26 is shutdown switch, and low liquid level
Switch 27 is open type switch, and high level switch 26 is normally closed switch.
Referring to fig. 4, agitating device 4 includes air blower 41, the first connecting tube 42 and the second connecting tube 43.Air blower 41,
One connecting tube 42 and the second connecting tube 43 are arranged at the outside of culture tank 2, and one end of the first connecting tube 42 and air blower 41 are taken out
Port connection, the other end of the first connecting tube 42 is connected to the upside of fluid reservoir 1, and the first connecting tube 42 is connected to fluid reservoir 1
Place is higher than the height of nutrient solution in fluid reservoir 1.One end of second connecting tube 43 is connected to the blowing mouth of air blower 41, the second connection
The other end of pipe 43 is connected to the downside of fluid reservoir 1.Also, the height of air blower 41 is higher than the height of nutrient solution in fluid reservoir 1.
In real work, the air of 1 upside of fluid reservoir is extracted by air blower 41, and delivers the air to the downside of fluid reservoir 1, by air
Nutrient solution is impacted, and is floated by gas and stirs nutrient solution.
Agitating device 4 further includes stirring pipe group 44, and stirring pipe group 44 includes the branch pipes 45 for being in several settings that are interconnected,
The upper of branch pipe 45 is provided with and communicated with gas distribution pipe 46 in several, and stirring pipe group 44 and the second communicating pipe take out far from air blower 41
The one end in air port is connected to.Gas distribution pipe 46 is located at the downside of branch pipe 45 and is directed toward the bottom wall of fluid reservoir 1.In real work, through gas distribution
The gas that pipe 46 is discharged will impact first the bottom wall of fluid reservoir 1, to reduce the case where generation of 1 bottom wall of fluid reservoir precipitates；
Also, after nutrient solution is completely exhausted out in fluid reservoir 1, the nutrient solution in stirring pipe group 44 can be discharged under the blowing of air-flow to be stirred
Mix pipe group 44.
Referring to Fig. 1, enter the generation of the situation in culture tank 2 to reduce low temperature nutrient solution under cold snap, supply pipe 3 is close
Having heaters 34 is arranged in the side of culture tank 2.It, can be by 34 pairs of the heater battalion Jing Guo supply pipe 3 when being in cold snap
Nutrient solution is heated.
Embodiment two:
Referring to Fig. 5, a kind of cell factory pipe-line system, the difference with embodiment one is: the downside of fluid reservoir 1 is provided with confession
To pump 7, the liquid pumping hole of supply pump 7 is connected to the bottom of fluid reservoir 1, and the leakage fluid dram of supply pump 7 is provided with and communicated with perfusion tube 71, is appointed
One corresponding connection is connected in parallel on perfusion tube 71 to the supply pipe 3 of culture tank 2 far from one end of culture tank 2.In real work, supply
The nutrient solution in fluid reservoir 1 is extracted to pump 7 to supply through supply pipe 3 into culture tank 2.
The Jar opening sealer of fluid reservoir 1 has closing cap 8, and snorkel 81 is provided with and communicated on closing cap 8, is set on snorkel 81
It is equipped with third solenoid valve 82, snorkel 81 is provided with and communicated with air filter 83 far from one end of closing cap 8.When fluid reservoir 1 is right
When culture tank 2 fills into nutrient solution, staff can open third solenoid valve 82, so that with the decline of liquid level in fluid reservoir 1
And ventilate, and the air entered in fluid reservoir 1 by 83 Duis of air filter is filtered, it reduces external impurities and enters liquid storage
The case where pollution nutrient solution of tank 1, occurs.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of cell factory pipe-line system, including fluid reservoir (1) and culture tank (2), the culture tank (2) are provided with several
It is a, and supply pipe (3) are communicated between any culture tank (2) and fluid reservoir (1), which is characterized in that any confession
It is provided with electric flow control valve (31) to pipe (3) close to the side of culture tank (2), any supply pipe (3) is electronic
Flow control valve (31) is provided with feedback control flow sensor (32), the feedback control close to the side of culture tank (2)
Flow sensor (32) is electrically connected with corresponding electric flow control valve (31) and controls electric flow control valve (31)；
In real work, fluid reservoir (1) actually enters culture not less than nutrient solution to the maximum feed speed of each supply pipe (3)
The speed of tank (2).
2. a kind of cell factory pipe-line system according to claim 1, which is characterized in that setting in the culture tank (2)
Have filter membrane (21), and the filter membrane (21) will be divided into culture chamber (22) and drain chamber (23), institute in culture tank (2)
The lower wall for stating drain chamber (23) is provided with and communicated with drain pipe (24), is communicated with control valve on the drain pipe (24).
3. a kind of cell factory pipe-line system according to claim 2, which is characterized in that the supply pipe (3) is far from training
One end connection of tank (2) is supported in side of culture chamber (22) far from drain pipe (24).
4. a kind of cell factory pipe-line system according to claim 2, which is characterized in that the control valve is the first electromagnetism
Valve (25), high level switch (26) and low liquid level switch (27) are provided in the culture tank (2), and the supply pipe (3) is close
The side of culture tank (2) is provided with second solenoid valve (33), and both the high level switch (26) and low liquid level switch (27) are
It is electrically connected with second solenoid valve (33), and the high level switch (26) and low liquid level switch (27) jointly control the second electromagnetism
Valve (33)；
The low liquid level switch (27) and the first solenoid valve (25) is electrically connected and controls the first solenoid valve (25)；
When needing to Additional nutrient solution in culture tank (2), staff opens the first solenoid valve (25), will be in culture tank (2)
Part nutrient solution be discharged culture tank (2)；It is low when nutrient solution liquid level reaches low liquid level switch (27) contact in culture tank (2)
Liquid-level switch (27) controls the first solenoid valve (25) and closes, controls the second control valve opening, at this point, culture tank (2) stops discharge
Nutrient solution, supply pipe (3) supplying nutrition liquid in culture tank (2)；When nutrient solution liquid level reaches high level switch in culture tank (2)
(26) when contact, the second control valve is closed, and supply pipe (3) stops the supplying nutrition liquid in culture tank (2).
5. a kind of cell factory pipe-line system according to claim 1, which is characterized in that the tank mouth of the fluid reservoir (1)
It is sealed with closing cap (8), is provided with and communicated with snorkel (81) on the closing cap (8), is provided on the snorkel (81)
Third solenoid valve (82), the snorkel (81) are provided with and communicated with air filter (83) far from the one end of closing cap (8).
6. a kind of cell factory pipe-line system according to claim 1, which is characterized in that be arranged on the fluid reservoir (1)
There is the agitating device (4) for stirring fluid reservoir (1) interior nutrient solution.
7. a kind of cell factory pipe-line system according to claim 6, which is characterized in that the agitating device (4) includes
Air blower (41), the first connecting tube (42) and the second connecting tube (43), the air blower (41), the first connecting tube (42) and second
Connecting tube (43) is arranged at the outside of culture tank (2), one end of first connecting tube (42) and air blower (41) bleeding point
Connection, the other end of first connecting tube (42) is connected to the upside of fluid reservoir (1), and first communicating pipe and fluid reservoir
(1) connectivity part is higher than the height of fluid reservoir (1) interior nutrient solution；
One end of second communicating pipe is connected to the blowing mouth of air blower (41), the other end and liquid storage of second communicating pipe
The downside of tank (1) is connected to；
Also, the height of the air blower (41) is higher than the height of fluid reservoir (1) interior nutrient solution.
8. a kind of cell factory pipe-line system according to claim 7, which is characterized in that the agitating device (4) is also wrapped
It includes stirring pipe group (44), the stirring pipe group (44) includes in the branch pipe (45) of several settings that are interconnected, the branch pipe
(45) upper is provided with and communicated with gas distribution pipe (46) in several, and the stirring pipe group (44) and the second communicating pipe are far from air blast
One end of machine (41) suction opening is connected to.
9. a kind of cell factory pipe-line system according to claim 8, which is characterized in that the gas distribution pipe (46) is located at branch
It manages the downside of (45) and is directed toward the bottom wall of fluid reservoir (1).
10. a kind of cell factory pipe-line system according to claim 1, which is characterized in that any supply pipe (3) is leaned on
The side of nearly culture tank (2) is provided with heater (34).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910727689.0A CN110423690B (en) | 2019-08-08 | 2019-08-08 | Cell factory pipeline system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910727689.0A CN110423690B (en) | 2019-08-08 | 2019-08-08 | Cell factory pipeline system |
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| CN110423690B CN110423690B (en) | 2022-08-30 |
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Citations (5)
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|---|---|---|---|---|
| JPH10230297A (en) * | 1997-02-17 | 1998-09-02 | Ebara Corp | Treatment of bottom sludge and device therefor |
| CN102286374A (en) * | 2011-07-26 | 2011-12-21 | 江苏农林职业技术学院 | Plant tissue liquid automatic intermittent submerged circulative culture device |
| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
| CN204162717U (en) * | 2014-10-17 | 2015-02-18 | 漯河医学高等专科学校 | A kind of microorganism continuous culture device |
| CN207767172U (en) * | 2018-01-16 | 2018-08-28 | 山东农业大学 | A kind of nutrient solution suitable for water planting is quantitative automatic to add system |
-
2019
- 2019-08-08 CN CN201910727689.0A patent/CN110423690B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10230297A (en) * | 1997-02-17 | 1998-09-02 | Ebara Corp | Treatment of bottom sludge and device therefor |
| CN102286374A (en) * | 2011-07-26 | 2011-12-21 | 江苏农林职业技术学院 | Plant tissue liquid automatic intermittent submerged circulative culture device |
| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
| CN204162717U (en) * | 2014-10-17 | 2015-02-18 | 漯河医学高等专科学校 | A kind of microorganism continuous culture device |
| CN207767172U (en) * | 2018-01-16 | 2018-08-28 | 山东农业大学 | A kind of nutrient solution suitable for water planting is quantitative automatic to add system |
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| CN110423690B (en) | 2022-08-30 |
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Address after: 201500 building 11, No. 10, Lane 855, Jinzheng Road, Jinshan Industrial Zone, Jinshan District, Shanghai Patentee after: Shanghai Qingsai Biotechnology Co.,Ltd. Patentee after: SHANGHAI JIEWEI BIOLOGICAL ENGINEERING Co.,Ltd. Address before: 201500 building 11, No. 10, Lane 855, Jinzheng Road, Jinshan Industrial Zone, Jinshan District, Shanghai Patentee before: Shanghai Qingsai Biotechnology Co.,Ltd. Patentee before: SHANGHAI JIEWEI BIOLOGICAL ENGINEERING Co.,Ltd. |