CN108456620A - A kind of novel methane oxidation bacteria culture test system - Google Patents
A kind of novel methane oxidation bacteria culture test system Download PDFInfo
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- CN108456620A CN108456620A CN201810042502.9A CN201810042502A CN108456620A CN 108456620 A CN108456620 A CN 108456620A CN 201810042502 A CN201810042502 A CN 201810042502A CN 108456620 A CN108456620 A CN 108456620A
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- 238000012360 testing method Methods 0.000 title claims abstract description 58
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 46
- 241000894006 Bacteria Species 0.000 title claims abstract description 21
- 230000003647 oxidation Effects 0.000 title claims abstract description 16
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 230000007246 mechanism Effects 0.000 claims abstract description 26
- 238000002347 injection Methods 0.000 claims abstract description 20
- 239000007924 injection Substances 0.000 claims abstract description 20
- 238000005070 sampling Methods 0.000 claims abstract description 15
- 239000012530 fluid Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000000565 sealant Substances 0.000 claims abstract description 8
- 239000013589 supplement Substances 0.000 claims abstract description 8
- 239000002689 soil Substances 0.000 claims description 12
- 238000003860 storage Methods 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 2
- 238000001802 infusion Methods 0.000 abstract description 4
- 239000000243 solution Substances 0.000 abstract description 3
- 239000012531 culture fluid Substances 0.000 abstract description 2
- 238000013461 design Methods 0.000 abstract description 2
- 239000008236 heating water Substances 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 238000005086 pumping Methods 0.000 abstract description 2
- 239000007789 gas Substances 0.000 description 8
- 230000008859 change Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003345 natural gas Substances 0.000 description 2
- 108010027322 single cell proteins Proteins 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- NLOAOXIUYAGBGO-UHFFFAOYSA-N C.[O] Chemical compound C.[O] NLOAOXIUYAGBGO-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004177 carbon cycle Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000011090 industrial biotechnology method and process Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Computer Hardware Design (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of novel methane oxidation bacteria cultures to test system, it is characterised in that：Including injection device, culture apparatus, sampler and control device；By first will be in test cabinet and entire injection device is evacuated by vacuum pumping pump outside test cabinet in the present invention, it recycles high pressure fluid injection metering pump and liquid booster pump that methane oxidation bacteria culture fluid is injected into test cabinet to be stirred to testing indoor methane oxidation culture solution by rotating mechanism, heating water bath is carried out by water leg and the water-bath circulatory system, by being sampled test in pressure-retaining system, whole process is realized by control system；Sealant supplement cylinder can carry out fluid infusion without opening test cabinet fluid infusion automatically, and the volume of test cabinet is automatically-calibrated；Use modularized design, each unit that can gather use in the present invention；Compact-sized simple to operation, sampling and testing is efficient and convenient.
Description
Technical field
The present invention relates to mushroom culture testing fields more particularly to a kind of novel methane oxidation bacteria culture to test system.
Background technology
Methane-oxidizing bacteria is a kind of special microorganism, it with methane be unique carbon source and the energy, methane-oxidizing bacteria
There is important application value in nature carbon cycle and industrial biotechnology.It is started from using methane production single cell protein
In the 70's, it is the natural gas Protein assay for producing 300 tons per year that Shell Co. Ltd of Britain in 1970 establishes a scale in Britain
Device, 1974, Shell Co. Ltd announced to establish a larger experimental rig in Britain, and prepares in Dutch A Musi
One scale of special red preparation is to produce the development plan of 100,000 tons of devices per year.But in 1976, Shell Co. Ltd determined to stop being somebody's turn to do
Development plan is simultaneously infinitely postponed, at that time the reason of be attributed to：The price of soybean and corn is very low.Norwegian National oil in 1999
Company jointly invests to have built with Nycomed Amer-sham companies of Britain first applies mechanically natural gas production methane oxygen in the world
Change the commercial plant of bacterium single cell protein.This covering device has the ability for producing 10000 tons of bioproteins per year.That produces is unicellular
Albumen has agricultural cooperation in Norway at present containing 70% protein, 12% carbohydrate, 10% fat and 8% minerals
Society sells as feed.
But since the solubility of methane in aqueous solution is low, methane-oxidizing bacteria is low using the efficiency of methane, causes
Methane oxidizing bacteria growth rate is slow, and gained cell concentration is low, therefore it becomes the bottleneck of methane-oxidizing bacteria commercial Application, by force
Change gas-liquid mass transfer be the key that establish one of methane-oxidizing bacteria high density fast culture, traditional solution be increase gas velocity and
Stir speed (S.S.), but increase gas velocity and stir speed (S.S.), on the one hand cause a large amount of foams to generate, reduces reactor utilization ratio, separately
On the one hand a large amount of power can be consumed, cost is increased.
Invention content
The technical problem to be solved in the present invention is to provide it is a kind of can in real time can be with sampling and measuring methane oxidation culture bacterium
System is tested in the novel methane oxidation bacteria culture for cultivating situation.
In order to solve the above technical problems, the technical scheme is that：A kind of novel methane oxidation bacteria culture test system,
Its innovative point is：Including injection device, culture apparatus, sampler and control device；
The injection device includes liquid injecting mechanism and gas injecting mechanism；The liquid injecting mechanism and gas injecting mechanism
It is connected on culture apparatus by pipeline respectively；The liquid injecting mechanism includes high pressure fluid injection metering pump, piston liquid storage container
And sealant supplement cylinder；The high pressure fluid injection metering pump is connected by the first valve with the input terminal of piston liquid storage container, the high pressure injection
The output end of liquid metering pump has been sequentially connected in series the second valve and liquid feed valve, and the output end of the liquid feed valve is connected with culture apparatus；
The sealant supplement cylinder is by third valve in parallel between the second valve and piston liquid storage container；
The gas injecting mechanism includes piston air container unit, liquid booster pump and intake valve；The piston air container
There are several to be mutually in parallel to form the both ends of piston air container group and piston air container unit and be both provided with control for unit
The output end of valve, the liquid booster pump is connected with the input terminal of piston air container group, and one end of the intake valve is connected on
On the output end of piston air container group, the other end of intake valve is connected on culture apparatus；
The culture apparatus includes vacuum device, test cabinet, water leg and rotating mechanism；The vacuum device includes vacuum
Pump and evacuation valve；The vacuum pump is connected with one end of evacuation valve, and the other end of evacuation valve is connected on test cabinet；The water
Folder is set on the outer wall of test cabinet, and water leg is connected with a water-bath circulatory system；The rotating mechanism is arranged in test cabinet
Side and extend in test cabinet and be stirred in test cabinet；
The sampler is connected to by sampling valve on test cabinet, and the sampler includes piston type soil sampler, liquid collecting tank
And ballast pump；One end of the piston type soil sampler is connected with one end of sampling valve, the other end string of the piston type soil sampler
It is associated on ballast pump；The liquid collecting tank is connected in parallel between the output end of sampling valve and piston type soil sampler；
The control device includes servo-control system and PID control system；Described servo-control system one end and PID control system
Unified end series connection, the other end of the servo-control system are connected on test cabinet, and the other end of PID control system is connected to flat
On weighing apparatus pump.
Further, the piston type soil sampler, liquid collecting tank and ballast pump are arranged in the same pressure-retaining system.
Further, the test cabinet is pressure piston cylinder operator, and the test cabinet keeps the volume of test cabinet by piston
And pressure；Temperature detection table is also associated on the test cabinet.
The advantage of the invention is that：
1）In the present invention by outside test cabinet by vacuum pumping pump first by test cabinet and entire injection device is evacuated,
Recycle high pressure fluid injection metering pump and liquid booster pump that methane oxidation bacteria culture fluid is injected into test cabinet and passes through rotating mechanism
It is stirred to testing indoor methane oxidation culture solution, heating water bath is carried out by water leg and the water-bath circulatory system, is passed through
Test is sampled in pressure-retaining system, whole process is realized by control system；Sealant supplement cylinder can carry out fluid infusion automatically and be not necessarily to
Test cabinet fluid infusion is opened, the volume of test cabinet is automatically-calibrated；Use modularized design, each unit that can gather and make in the present invention
With；Compact-sized simple to operation, sampling and testing is efficient and convenient.
Description of the drawings
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Fig. 1 is that the schematic diagram of system is tested in a kind of novel methane oxidation bacteria culture of the present invention.
Specific implementation mode
The following examples can make professional and technical personnel that the present invention be more fully understood, but therefore not send out this
It is bright to be limited among the embodiment described range.
System is tested in a kind of novel methane oxidation bacteria culture as shown in Figure 1, including injection device 1, culture apparatus 2, is taken
Sampling device 3 and control device 4.
Injection device 1 includes liquid injecting mechanism 11 and gas injecting mechanism 12；The liquid injecting mechanism 11 and gas
Injecting mechanism 12 is connected to by pipeline on culture apparatus 2 respectively；The liquid injecting mechanism 11 includes high pressure fluid injection metering pump
13, piston liquid storage container 14 and sealant supplement cylinder 15；The high pressure fluid injection metering pump 13 passes through the first valve 16 and piston liquid storage container
14 input terminal series connection, the output end of the high pressure fluid injection metering pump 13 have been sequentially connected in series the second valve 17 and liquid feed valve 18, institute
The output end for stating liquid feed valve 18 is connected with culture apparatus 2；The sealant supplement cylinder 15 is connected in parallel on the second valve 17 by third valve 19
Between piston liquid storage container 14.
Gas injecting mechanism 12 includes piston air container unit 121, liquid booster pump 122 and intake valve 123；The work
Plug air container unit 121 is mutually in parallel to form piston air container group and piston air container unit 121 with several
Both ends are both provided with control valve, and the output end of the liquid booster pump 122 is connected with the input terminal of piston air container group, described
One end of intake valve 123 is connected on the output end of piston air container group, and the other end of intake valve 123 is connected on culture apparatus
On 2.
Culture apparatus 2 includes vacuum device 21, test cabinet 22, water leg 23 and rotating mechanism 24；The evacuator
Structure 21 includes vacuum pump 211 and evacuation valve 212；The vacuum pump 211 is connected with one end of evacuation valve 212, evacuation valve 212
The other end is connected on test cabinet 22；The water leg 23 is arranged on the outer wall of test cabinet 22, and water leg 23 is followed with a water-bath
Loop system 25 is connected；The rotating mechanism 24 is arranged the side in test cabinet 22 and extends in test cabinet 22 and in test cabinet 22
It is stirred.
Sampler 3 is connected to by sampling valve 31 on test cabinet 22, and the sampling fills 3 and sets including piston type soil sampler
32, liquid collecting tank 33 and ballast pump 34；One end of the piston type soil sampler 32 is connected with one end of sampling valve 31, the piston type
The other end of sampler 32 is connected on ballast pump 34；The liquid collecting tank 33 is connected in parallel on the output end of sampling valve 31 and piston type takes
Between sample device 31.
Control device 4 includes servo-control system 41 and PID control system 42；41 one end of the servo-control system and
42 one end of PID control system is connected, and the other end of the servo-control system 41 is connected on test cabinet 22, PID control system
42 other end is connected on ballast pump 34.
Piston type soil sampler 32, liquid collecting tank 33 and ballast pump 34 are arranged in the same pressure-retaining system.
Test cabinet 22 is pressure piston cylinder operator, and the test cabinet 22 keeps the volume and pressure of test cabinet by piston；Institute
It states and is also associated with temperature detection table on test cabinet 22.
It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, above-described embodiment and explanation
Merely illustrating the principles of the invention described in book, without departing from the spirit and scope of the present invention, the present invention also has
Various changes and modifications, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention
It is defined by the appending claims and its equivalent thereof.
Claims (3)
1. system is tested in a kind of novel methane oxidation bacteria culture, it is characterised in that：Including injection device, culture apparatus, sampling dress
It sets and control device；
The injection device includes liquid injecting mechanism and gas injecting mechanism；The liquid injecting mechanism and gas injecting mechanism
It is connected on culture apparatus by pipeline respectively；The liquid injecting mechanism includes high pressure fluid injection metering pump, piston liquid storage container
And sealant supplement cylinder；The high pressure fluid injection metering pump is connected by the first valve with the input terminal of piston liquid storage container, the high pressure injection
The output end of liquid metering pump has been sequentially connected in series the second valve and liquid feed valve, and the output end of the liquid feed valve is connected with culture apparatus；
The sealant supplement cylinder is by third valve in parallel between the second valve and piston liquid storage container；
The gas injecting mechanism includes piston air container unit, liquid booster pump and intake valve；The piston air container
There are several to be mutually in parallel to form the both ends of piston air container group and piston air container unit and be both provided with control for unit
The output end of valve, the liquid booster pump is connected with the input terminal of piston air container group, and one end of the intake valve is connected on
On the output end of piston air container group, the other end of intake valve is connected on culture apparatus；
The culture apparatus includes vacuum device, test cabinet, water leg and rotating mechanism；The vacuum device includes vacuum
Pump and evacuation valve；The vacuum pump is connected with one end of evacuation valve, and the other end of evacuation valve is connected on test cabinet；The water
Folder is set on the outer wall of test cabinet, and water leg is connected with a water-bath circulatory system；The rotating mechanism is arranged in test cabinet
Side and extend in test cabinet and be stirred in test cabinet；
The sampler is connected to by sampling valve on test cabinet, and the sampler includes piston type soil sampler, liquid collecting tank
And ballast pump；One end of the piston type soil sampler is connected with one end of sampling valve, the other end string of the piston type soil sampler
It is associated on ballast pump；The liquid collecting tank is connected in parallel between the output end of sampling valve and piston type soil sampler；
The control device includes servo-control system and PID control system；Described servo-control system one end and PID control system
Unified end series connection, the other end of the servo-control system are connected on test cabinet, and the other end of PID control system is connected to flat
On weighing apparatus pump.
2. system is tested in a kind of novel methane oxidation bacteria culture according to claim 1, it is characterised in that：The piston type
Sampler, liquid collecting tank and ballast pump are arranged in the same pressure-retaining system.
3. system is tested in a kind of novel methane oxidation bacteria culture according to claim 1, it is characterised in that：The test cabinet
To press piston cylinder operator, the test cabinet to keep the volume and pressure of test cabinet by piston；It is also associated on the test cabinet
Temperature detection table.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810042502.9A CN108456620A (en) | 2018-01-17 | 2018-01-17 | A kind of novel methane oxidation bacteria culture test system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810042502.9A CN108456620A (en) | 2018-01-17 | 2018-01-17 | A kind of novel methane oxidation bacteria culture test system |
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| Publication Number | Publication Date |
|---|---|
| CN108456620A true CN108456620A (en) | 2018-08-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810042502.9A Pending CN108456620A (en) | 2018-01-17 | 2018-01-17 | A kind of novel methane oxidation bacteria culture test system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114292725A (en) * | 2021-11-25 | 2022-04-08 | 浙江大学滨海产业技术研究院 | In-situ culture methane-oxidizing bacteria and pressure-maintaining sampling device and detection system and method thereof |
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|---|---|---|---|---|
| CN101113408A (en) * | 2007-05-31 | 2008-01-30 | 华南农业大学 | Intestinal flora isolated culturing device |
| DE102007063091A1 (en) * | 2007-12-28 | 2009-07-02 | Right-Way-Technologies Gmbh & Co. Kg | Method and device to produce biogas with high methane rate, by dosingly supplying hydrogen-producing bacteria, which are cultured in hydrogen and methane fermenter, and dosingly introducing gaseous hydrogen into methane fermenter |
| CN204162717U (en) * | 2014-10-17 | 2015-02-18 | 漯河医学高等专科学校 | A kind of microorganism continuous culture device |
| CN104777057A (en) * | 2015-03-24 | 2015-07-15 | 中国矿业大学 | Supercritical CO2 injection and coalbed methane enhanced displacement simulation test device |
| US9598936B1 (en) * | 2015-10-12 | 2017-03-21 | China University Of Petroleum (East China) | Apparatus and method for monitoring hydrate decomposition area under different drilling and production processes |
-
2018
- 2018-01-17 CN CN201810042502.9A patent/CN108456620A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101113408A (en) * | 2007-05-31 | 2008-01-30 | 华南农业大学 | Intestinal flora isolated culturing device |
| DE102007063091A1 (en) * | 2007-12-28 | 2009-07-02 | Right-Way-Technologies Gmbh & Co. Kg | Method and device to produce biogas with high methane rate, by dosingly supplying hydrogen-producing bacteria, which are cultured in hydrogen and methane fermenter, and dosingly introducing gaseous hydrogen into methane fermenter |
| CN204162717U (en) * | 2014-10-17 | 2015-02-18 | 漯河医学高等专科学校 | A kind of microorganism continuous culture device |
| CN104777057A (en) * | 2015-03-24 | 2015-07-15 | 中国矿业大学 | Supercritical CO2 injection and coalbed methane enhanced displacement simulation test device |
| US9598936B1 (en) * | 2015-10-12 | 2017-03-21 | China University Of Petroleum (East China) | Apparatus and method for monitoring hydrate decomposition area under different drilling and production processes |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114292725A (en) * | 2021-11-25 | 2022-04-08 | 浙江大学滨海产业技术研究院 | In-situ culture methane-oxidizing bacteria and pressure-maintaining sampling device and detection system and method thereof |
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