CN212982940U - Tryptophan fermentation apparatus for producing - Google Patents
Tryptophan fermentation apparatus for producing Download PDFInfo
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- CN212982940U CN212982940U CN202021063654.6U CN202021063654U CN212982940U CN 212982940 U CN212982940 U CN 212982940U CN 202021063654 U CN202021063654 U CN 202021063654U CN 212982940 U CN212982940 U CN 212982940U
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Abstract
A tryptophan fermentation production device comprises a tryptophan fermentation tank, a sugar supplement tank, a material supplement tank, a seed tank, a defoaming agent tank and a belt discharge tank; the tryptophan fermentation tank is respectively communicated with the sugar supplement tank, the seed tank, the defoamer tank and the belt discharge tank through pipelines, and valves are arranged on the pipelines communicated among the tryptophan fermentation tank, the sugar supplement tank, the seed tank, the defoamer tank and the belt discharge tank; the utility model discloses can be according to the particular case among the tryptophan fermentation process, fill sugar, benefit culture medium, supply the bacterial in a flexible way, benefit defoaming agent defoaming, take and put or even put the operation such as, not only avoided high liquid level to escape liquid easily and stir the high problem of energy consumption, can make full use of auxiliary raw and other materials in addition, improve the productivity of bacterial, reduction in production cost reduces the inhibitory action that the result brought, has obvious advantage to improving tryptophan fermentation production technical level.
Description
Technical Field
The utility model relates to a tryptophan fermentation production device belongs to amino acid production technical field.
Background
Tryptophan is one of eight essential amino acids in human and animal life activities, exists in a free state or a combined state in organisms, plays a very important role in the growth, development and metabolism of human and animals, is called as a second essential amino acid, and is widely applied to various aspects such as medicines, foods, feeds and the like.
The main production methods of tryptophan include a chemical synthesis method, an enzymatic catalysis method and a direct fermentation method. The direct fermentation method uses cheap raw materials such as glucose, cane molasses and the like as carbon sources, and utilizes excellent tryptophan production strains to produce tryptophan. The main product in the process of producing tryptophan by biological fermentation is tryptophan, so that the downstream separation and purification process is easier to realize. With the application of recombinant DNA technology in microbial breeding, the production of tryptophan by direct microbial fermentation is a mainstream industrial production method, and the production method gradually becomes a mainstream production method with the advantages of low cost, easy popularization of industrial production and the like.
The technical level of the prior fermentation method for producing tryptophan is greatly developed, in the fermentation process, tryptophan crystals are separated out from fermentation liquor in a large amount of crystallization, the separated crystals easily block an air pipeline, and dissolved oxygen in the fermentation process is reduced to influence the fermentation level of tryptophan. Due to the progress of the strain technology, the activity of the tryptophan high-yield strain is strong, the fermentation liquor is easily at a higher liquid level through the supplement operations of sugar supplement, culture medium supplement and the like in the fermentation process, the fermentation liquor is easy to escape, the stirring power is increased, the fermentation can be ended in advance seriously, the production capacity and the fermentation equipment of the strain cannot be utilized to the maximum extent, and a series of problems of environmental protection, energy waste and the like can be caused. .
Disclosure of Invention
In order to overcome the defects in the prior art, the utility model aims to provide a tryptophan fermentation production device.
In order to achieve the above objects and other related objects, the present invention provides a technical solution: a tryptophan fermentation production device comprises a tryptophan fermentation tank, a sugar supplement tank, a material supplement tank, a seed tank, a defoaming agent tank and a belt discharge tank; the tryptophan fermentation tank is respectively communicated with the sugar supplement tank, the seed tank, the defoaming agent tank and the band discharging tank through pipelines, wherein the pipelines among the tryptophan fermentation tank, the sugar supplement tank, the seed tank and the defoaming agent tank are communicated with an inlet formed in the top of the tryptophan fermentation tank, and the pipeline between the tryptophan fermentation tank and the band discharging tank is communicated with an outlet formed in the bottom of the tryptophan fermentation tank; and simultaneously, the tryptophan fermentation tank is provided with valves on pipelines communicated with the sugar supplementing tank, the material supplementing tank, the seed tank, the defoaming agent tank and the belt discharging tank.
Preferably, the tryptophan fermentation tank, the sugar supplementing tank, the material supplementing tank, the seed tank and the defoaming agent tank are all provided with an air inlet pipeline and an air exhaust pipeline which are communicated with the inside.
Preferably, the tryptophan fermentation tank, the sugar supplement tank, the material supplement tank, the seed tank and the defoaming agent tank are all provided with steam pipelines communicated with the inside.
Preferably, the valve is a manual valve.
Preferably, the valve is an automatic valve.
Preferably, the air inlet pipeline, the air outlet pipeline and the steam pipeline are all provided with control valves.
Because of the application of the technical scheme, compared with the prior art, the utility model the advantage that has as follows:
1. the method can be used for performing sugar supplement, material supplement, strain supplement and foam enemy supplement in the tryptophan fermentation process, can also be used for discharging part of fermentation liquor in the tryptophan fermentation process, can be used for intermittent operation and continuous operation, realizes the continuous or semi-continuous fermentation of the tryptophan, can control the liquid level of the fermentation liquor in a reasonable range, not only avoids the problems that the high liquid level is easy to escape from the liquid and the stirring energy consumption is high, but also can exert the production capacity of strains to the maximum extent, and maximizes the utilization rate of equipment.
2. The crystallized tryptophan in the tryptophan fermentation liquid can be discharged from the fermentation tank in time, the inhibition effect brought by the product is reduced, the yield of the tryptophan is improved, meanwhile, the pipeline blockage caused by precipitation and enrichment of a large amount of tryptophan crystals can be avoided, and the abnormal fermentation condition caused by pipeline blockage is avoided.
3. According to the specific conditions in the fermentation process of tryptophan, the operations of supplementing sugar, supplementing culture medium, supplementing strains, supplementing defoaming agent, and carrying or continuously releasing can be flexibly carried out, so that the raw and auxiliary materials can be fully utilized, the production capacity of the strains is improved, the production cost is reduced, and the method has obvious advantages in improving the production technical level.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
In the attached figures, the tryptophan fermentation tank 1, the sugar supplement tank 2, the material supplement tank 3, the seed tank 4, the defoaming agent tank 5, the belt discharge tank 6, the air inlet pipeline 7, the exhaust pipeline 8 and the steam pipeline 9.
Detailed Description
The following description is provided for illustrative purposes, and other advantages and features of the present invention will become apparent to those skilled in the art from the following detailed description.
Please refer to fig. 1. It should be understood that the structure, ratio, size and the like shown in the drawings attached to the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and are not used for limiting the limit conditions that the present invention can be implemented, so that the present invention has no technical essential meaning, and any structure modification, ratio relationship change or size adjustment should still fall within the scope that the technical content disclosed in the present invention can cover without affecting the function that the present invention can produce and the purpose that the present invention can achieve. Meanwhile, the terms such as "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for convenience of description, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof may be made without substantial technical changes, and the present invention is also regarded as the scope of the present invention.
As shown in figure 1, a tryptophan fermentation production device comprises a tryptophan fermentation tank, a sugar supplement tank, a material supplement tank, a seed tank, a defoaming agent tank and a belt discharge tank; the tryptophan fermentation tank is respectively communicated with the sugar supplement tank, the seed tank, the defoaming agent tank and the band discharging tank through pipelines, wherein the pipelines among the tryptophan fermentation tank, the sugar supplement tank, the seed tank and the defoaming agent tank are communicated with an inlet formed in the top of the tryptophan fermentation tank, and the pipeline between the tryptophan fermentation tank and the band discharging tank is communicated with an outlet formed in the bottom of the tryptophan fermentation tank; meanwhile, valves are arranged on pipelines communicated among the tryptophan fermentation tank, the sugar supplement tank, the seed tank, the defoaming agent tank and the belt discharge tank; the tryptophan fermentation production device designed by the scheme has the following principle: the seeding tank 4 is used for strain amplification culture, after the strain is cultured to the logarithmic growth phase, the strain is inoculated into the tryptophan fermentation tank 1 from the seeding tank 4, and fermentation culture is carried out in the tryptophan fermentation tank 1; in the fermentation culture process, according to the change of a fermentation culture medium and strains and different culture conditions, sterile sugar liquid is supplemented into the tryptophan fermentation tank 1 from the sugar supplement tank 2, other materials such as the culture medium and the like are supplemented into the tryptophan fermentation tank 1 from the sugar supplement tank 3, and fresh viable strains are supplemented into the tryptophan fermentation tank 1 from the seed tank 4; according to the condition of foam generated in the fermentation process, supplementing a defoaming agent from a defoaming agent tank 5 to the tryptophan fermentation tank 1 to eliminate fermentation foam; discharging part of fermentation liquor from the tryptophan fermentation tank 1 to the belt discharge tank 6 by integrating the liquid level condition and the crystal precipitation condition in the fermentation process, wherein continuous belt discharge or intermittent belt discharge can be adopted according to specific conditions;
the preferred embodiment is as follows:
the tryptophan fermentation tank, the sugar supplementing tank, the material supplementing tank, the seed tank and the defoaming agent tank are all provided with an air inlet pipeline 7 and an air exhaust pipeline 8 which are communicated with the inside; the exhaust duct 8 and the intake duct 7 are mainly used for the intake and exhaust operations of the interior of the tank described above.
The tryptophan fermentation tank, the sugar supplementing tank, the material supplementing tank, the seed tank and the defoaming agent tank are all provided with steam pipelines 9 communicated with the inside; the steam pipeline 9 is mainly used for performing air sterilization on the steam used in the tank body; before use, steam is introduced into the hollow tank body through the steam pipeline 9.
The valves on the pipelines for communicating the tryptophan fermentation tank and the sugar supplement tank, the tryptophan fermentation tank and the seed tank, the tryptophan fermentation tank and the antifoaming agent tank and the tryptophan fermentation tank and the belt discharge tank can be manual valves or automatic valves.
Control valves are arranged on the air inlet pipeline 7, the air outlet pipeline 8 and the steam pipeline 9.
The beneficial effect of this embodiment does: the tryptophan fermentation production device can perform operations such as sugar supplement, material supplement, strain supplement, foam enemy supplement and the like in the tryptophan fermentation process, can also discharge part of fermentation liquor in the tryptophan fermentation process, can perform intermittent operation and continuous operation, realizes continuous or semi-continuous fermentation of tryptophan, can control the liquid level of the fermentation liquor within a reasonable range, not only avoids the problems that high liquid level is easy to escape from the liquor and the stirring energy consumption is high, but also can exert the production capacity of strains to the maximum extent, and maximizes the utilization rate of equipment. The tryptophan fermentation production device can also discharge crystallized tryptophan in the tryptophan fermentation liquor from the fermentation tank in time, reduce the inhibition effect brought by the product, improve the yield of the tryptophan, simultaneously avoid pipeline blockage caused by precipitation and enrichment of a large amount of tryptophan crystals, and avoid abnormal fermentation conditions caused by pipeline blockage. Foretell tryptophan fermentation production device can be according to the particular case of tryptophan fermentation in-process, and the operation such as the nimble sugar of carrying on is mended, is mended culture medium, is supplyed the bacterial, is mended defoaming agent defoaming, is taken to put or is put in succession, can make full use of raw and auxiliary materials, improves the productivity of bacterial, reduction in production cost has obvious advantage to improving tryptophan fermentation production technology level.
The above embodiments are merely illustrative of the principles and effects of the present invention, and are not to be construed as limiting the invention. Modifications and variations can be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (6)
1. A tryptophan fermentation production device is characterized in that: comprises a tryptophan fermentation tank, a sugar supplement tank, a material supplement tank, a seed tank, a defoaming agent tank and a belt discharge tank; the tryptophan fermentation tank is respectively communicated with the sugar supplement tank, the seed tank, the defoaming agent tank and the band discharging tank through pipelines, wherein the pipelines among the tryptophan fermentation tank, the sugar supplement tank, the seed tank and the defoaming agent tank are communicated with an inlet formed in the top of the tryptophan fermentation tank, and the pipeline between the tryptophan fermentation tank and the band discharging tank is communicated with an outlet formed in the bottom of the tryptophan fermentation tank; and simultaneously, the tryptophan fermentation tank is provided with valves on pipelines communicated with the sugar supplementing tank, the material supplementing tank, the seed tank, the defoaming agent tank and the belt discharging tank.
2. The fermentation production apparatus of tryptophan according to claim 1, wherein: the tryptophan fermentation tank, the sugar supplementing tank, the material supplementing tank, the seed tank and the defoaming agent tank are all provided with an air inlet pipeline and an air exhaust pipeline which are communicated with the inside.
3. The fermentation production apparatus of tryptophan according to claim 2, wherein: the tryptophan fermentation tank, the sugar supplement tank, the material supplement tank, the seed tank and the defoaming agent tank are all provided with steam pipelines and communicated with the inside.
4. The fermentation production apparatus of tryptophan according to claim 1, wherein: the valve is a manual valve.
5. The fermentation production apparatus of tryptophan according to claim 1, wherein: the valve is an automatic valve.
6. The fermentation production apparatus of tryptophan according to claim 3, wherein: and the air inlet pipeline, the exhaust pipeline and the steam pipeline are all provided with control valves.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021063654.6U CN212982940U (en) | 2020-06-11 | 2020-06-11 | Tryptophan fermentation apparatus for producing |
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| CN202021063654.6U CN212982940U (en) | 2020-06-11 | 2020-06-11 | Tryptophan fermentation apparatus for producing |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114015559A (en) * | 2021-09-08 | 2022-02-08 | 安徽华恒生物科技股份有限公司 | High-efficiency valine semi-continuous fermentation method and complete equipment thereof |
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2020
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114015559A (en) * | 2021-09-08 | 2022-02-08 | 安徽华恒生物科技股份有限公司 | High-efficiency valine semi-continuous fermentation method and complete equipment thereof |
| CN114015559B (en) * | 2021-09-08 | 2023-06-23 | 安徽华恒生物科技股份有限公司 | Efficient valine semi-continuous fermentation method and complete equipment thereof |
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