CN103898171A - Efficient fermentation production process of sodium gluconate - Google Patents
Efficient fermentation production process of sodium gluconate Download PDFInfo
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- CN103898171A CN103898171A CN201410140262.8A CN201410140262A CN103898171A CN 103898171 A CN103898171 A CN 103898171A CN 201410140262 A CN201410140262 A CN 201410140262A CN 103898171 A CN103898171 A CN 103898171A
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Abstract
The invention relates to an efficient fermentation production process of a sodium gluconate. The efficient fermentation production process comprises the first step of seed culturing, the second step of inoculated fermentation, the third step of continuous fermentation, the fourth step of filtering, the fifth step of decoloring, the sixth step of crystallization, the seventh of separation and drying and the eighth step of packaging. In the third step, namely, the continuous fermentation step, when the online measured concentration of a sodium gluconate reducing sugar is less than or equal to 50g/L according to the terminal stage of fermentation of the second step, a glucose liquid and a nutrient are automatically replenished by 500g/L, and simultaneously, a fermentation liquor is discharged to a buffer tank from the bottom of a fermentation tank, and meanwhile, NaOH (in a content of 350g/L) is also replenished. The continuous fermentation step of the efficient fermentation production process has the advantages that the glucose liquid and the nutrient are replenished under online automatic control, long-term continuous fermentation of the glucose is realized, the time of seed culture and fermentation liquor sterilization is saved, and the production efficiency is improved.
Description
Technical field
The present invention relates to bioengineering field, specifically a kind of Sunmorl N 60S high-efficiency fermenting production technique.
background technology
Gluconic acid is as leavening agent, peptizer, intercalating agent, acidic flavoring agent and be widely used in the industries such as food, medicine, chemical industry, water treatment, building; Gluconic acid salt (as with the synthetic gluconate making of the metal oxide such as sodium, calcium, zinc, ferrous iron) can be used as foodstuff additive and accessory substance.
The production of gluconic acid mainly contains the methods such as microbe fermentation method, electrolytic process and catalyzed oxidation.Due to microbial method, mild condition, is energy-conservationly obviously widely adopted, and wherein microorganism fermentation has again fungi fermentation and fermentation using bacteria technique.Since Boot Shandong (Boutroux) utilizes the oxygenizement of microorganism glucose oxidase can be become to gluconic acid first, gluconic acid and its esters zymotechnique have had obvious progress, as block beautiful (Currie) etc. and utilize mould or aspergillus to set up modern aeration-agitation liquid fermentation technology, make the transformation efficiency of glucose reach 90%.Blom etc. have invented interpolation NaOH, have maintained the method production Sunmorl N 60S of pH value below 6.5, and inversion rate of glucose reaches more than 95%; China's ecliptic shakes etc. are inoculated 10% aspergillus niger seed liquor take glucose content as 30% fermention medium, 6.0~6.5, residual sugar can be down to below 1 g/L by stream hydro-oxidation sodium solution control pH value.In addition, there is report to find that by medium optimization pH value, temperature, fermentor tank rotating speed, substrate glucose concn affect inversion rate of glucose larger, obtain optimal processing parameter.Liu Zhong converges and waits the special pH Temperature Intelligent Control System designing for industrial bulk fermentation, has obtained good fermentation level.In addition, the short stalks of employing mould (Aureobasidium pullulans) such as A Disitaxiadisi (Anastassiadis) etc. in 2002 have been set up the continuous gluconic acid production technology of resting cell and immobilized cell, concentration can reach 26%, and the highest generating rate is 19g/L/h.
Therefore, in the time of gluconic acid and salt (as Sunmorl N 60S, calglucon etc.) fermentative production thereof, mainly adopt mold fermentation to carry out, its fermentation controlling method is mainly to control the methods such as pH, temperature, fermentor tank rotating speed, dissolved oxygen concentration (DO), remaining sugar concentration, and be that a collection of strain fermentation of every cultivation is a collection of, these methods all fail to realize the continous-stable fermentation of gluconic acid series.
In sum, at present this area lacks Sunmorl N 60S continuous ferment process, the method for therefore continuously fermenting in the urgent need to developing the Sunmorl N 60S of a kind of continuous, stable, high yield, less energy-consumption, on-line automatic control.
Summary of the invention
Object of the present invention is just to provide a kind of on-line automatic control and adds substrate glucose and nutrition agent, the Sunmorl N 60S high-efficiency fermenting production technique that can continuously ferment.For achieving the above object, the present invention has adopted following technical scheme:
Sunmorl N 60S high-efficiency fermenting production technique,
The first step, seed culture adds aspergillus niger strain and substratum to carry out seed culture in seed fermentation tank.
Second step, fermentation, 30% Glucose Liquid and nutrition agent are added to fermentor tank sterilizing, cultured seed is added to fermentor tank in 20% ratio, total add-on is to 50% ~ 60% of fermentor tank working volume, exhaust analyzer or mass spectrograph that online examination of glucose concentration stream adds carbon dioxide and oxygen in controller and on-line analysis fermentation tail gas are installed on fermentor tank, meanwhile, the mass flowmeter of inlet and outlet air and the true microorganism cells volume of culture sensor of fermentor tank are also installed.
The 3rd step, continuously ferment, aforementioned second step operation is fermented latter stage, carries out on-line automatic 500g/L Glucose Liquid and the nutrition agent added according to the Sunmorl N 60S concentration of reduced sugar of on-line determination, discharge fermented liquid to surge tank from fermentor tank bottom simultaneously, also add NaOH(content 350g/L simultaneously); Be specially, when in fermented liquid when concentration of reduced sugar≤50g/L, automatically add Glucose Liquid and nutrition agent, discharge fermented liquid simultaneously and make concentration of reduced sugar maintain 30g/L-50g/L, and maintain certain bacterium dense (dry weight 10g/L is to 20g/L) and fermentating liquid volume; Fermentor tank cubic capacity is made as V, and unit is L, and the speed of adding Glucose Liquid and nutrition agent is S1, and the speed of adding NaOH is S2, and fermentation liquid draining speed is S, and maximum liquid amount is controlled at fermentor tank cubic capacity below 83%,
In fermentor tank, 180-220 hours mycelium of fermented liquid fermentation are aging, stop adding Glucose Liquid and nutrition agent, to fermentation ends; Be discharged to fermented liquid in surge tank by fermentor tank and continue to ventilate, stir, adding NaOH, that reducing sugar is utilized is complete.
The 4th step, filters Plate Filtration.
The 5th step, decolouring, activated carbon decolorizing.
The 6th step, crystallization, 120-125 ℃ is concentrated into 70% above content, crystallisation by cooling.
The 7th step, separates, is dried, and separating machine is by crystal and liquid separation, and then fluidised bed drying, obtains Sunmorl N 60S finished product.
The 8th step, packing.
The operation of continuously fermenting of the present invention, Glucose Liquid and nutrition agent are added in on-line automatic control, have realized the long-time continuous fermentation of glucose, save spawn culture, fermented liquid sterilization time, have improved production efficiency.Technological principle of the present invention is equally applicable to the production of the gluconate such as calglucon, Zinc Gluconate.
Accompanying drawing explanation
Fig. 1 is process flow sheet of the present invention.
Embodiment
Further illustrate the present invention below in conjunction with embodiment.
Embodiment 1:
The first step, seed culture: in 15L culture tank, add glucose 2.5kg, corn steep liquor 60g, potassium primary phosphate 5.2g, urea 2g, defoamer 2ml, be settled to 7.5L, 115 ℃ of sterilizings 15 minutes, are then down to 38 ℃, add 250ml eggplant bottle bacteria suspension, control dissolved oxygen (DO) >=30, cultivate 15h and obtain seed liquor.
Second step, fermentation: in 50L fermentor tank, add glucose 4.0kg, magnesium sulfate 2.8g, urea 1.4g, defoamer 2ml, be settled to 13L, 115 ℃ of sterilizings 15 minutes, then be down to 38 ℃, cultured seed is added to fermentor tank in 20% ratio, regulate and control PH5.4 with 350g/L NaOH, start fermentation.
The 3rd step, continuously ferment:
(1), batching: in 30L feed supplement tank, add 500g/L glucose 14kg, corn steep liquor 50g, magnesium sulfate 12.5g, urea 6.8g, defoamer 1ml, is settled to 20L, 115 ℃ of sterilizings 30 minutes, are then down to 38 ℃, it is aseptic that to proceed to serum bottle for subsequent use.
(2), the control of continuously fermenting: aforementioned second step operation fermentation latter stage, carry out on-line automatic 500g/L Glucose Liquid and the nutrition agent added according to the Sunmorl N 60S concentration of reduced sugar of on-line determination, discharge fermented liquid simultaneously, be specially, when concentration of reduced sugar≤50g/L in fermented liquid automatically adds Glucose Liquid and contains nutrition agent, discharge fermented liquid simultaneously, make concentration of reduced sugar in fermented liquid maintain 30g/L between 50g/L, and maintaining the dense 10g/L (dry weight) of being of bacterium and maintaining fermentating liquid volume is 83% of fermentor tank cumulative volume, fermentor tank cubic capacity is made as V, unit is L, the speed of adding Glucose Liquid and nutrition agent is S1, add NaOH(content 350g/L) speed be S2, fermentation liquid draining speed is S,
The 185 hours mycelium that ferment are aging, stop flowing sugaring until fermentation ends.
Continuously ferment and discharge fermented liquid to surge tank simultaneously, continue to ventilate, stir, stream adds NaOH, and that reducing sugar is utilized is complete.
The 4th step, filtration: Plate Filtration.
The 5th step, decolouring: activated carbon decolorizing.
The 6th step, crystallization: 120-125 ℃ are concentrated into 70% above content, crystallisation by cooling.
The 7th step, separation, dry: separating machine is by crystal and liquid separation, and then fluidised bed drying, obtains Sunmorl N 60S finished product.
The 8th step, packing.
Embodiment 2:
The first step, seed culture: in 15L culture tank, add glucose 2.5kg, corn steep liquor 60g, potassium primary phosphate 5.2g, urea 2g, defoamer 2ml, be settled to 7.5L, 115 ℃ of sterilizings 15 minutes, are then down to 38 ℃, add 250ml eggplant bottle bacteria suspension, control dissolved oxygen (DO) >=30, cultivate 15h and obtain seed liquor.
Second step, fermentation: in 50L fermentor tank, add glucose 4.0kg, magnesium sulfate 2.8g, urea 1.4g, defoamer 2ml, be settled to 13L, 115 ℃ of sterilizings 15 minutes, then be down to 38 ℃, cultured seed is added to fermentor tank in 20% ratio, regulate and control PH5.4 with 350g/L NaOH, start fermentation.
The 3rd step, continuously ferment:
, batching: in 30L feed supplement tank, add 500g/L glucose 14kg, corn steep liquor 100g, magnesium sulfate 25g, urea 13.6g, defoamer 2ml, is settled to 20L, 115 ℃ of sterilizings 30 minutes, are then down to 38 ℃, it is aseptic that to proceed to serum bottle for subsequent use.
(2), the control of continuously fermenting: aforementioned second step operation fermentation latter stage, carry out on-line automatic 500g/L Glucose Liquid and the nutrition agent added according to the Sunmorl N 60S concentration of reduced sugar of on-line determination, discharge fermented liquid simultaneously, be specially, when concentration of reduced sugar≤50g/L in fermented liquid adds Glucose Liquid and nutrition agent automatically, discharge fermented liquid simultaneously, make concentration of reduced sugar in fermented liquid maintain 30g/L between 50g/L, and maintaining the dense 20g/L (dry weight) of being of bacterium and maintaining fermentating liquid volume is 83% of fermentor tank cumulative volume, fermentor tank cubic capacity is made as V, unit is L, the speed of adding Glucose Liquid and nutrition agent is S1, add NaOH(content 350g/L) speed be S2, fermentation liquid draining speed is S,
The 218 hours mycelium that ferment are aging, stop flowing sugaring until fermentation ends.
In Continuous Fermentation Processes, discharge fermented liquid to surge tank simultaneously, continue to ventilate, stir, stream adds NaOH, and that reducing sugar is utilized is complete.
The 4th step, filtration: Plate Filtration.
The 5th step, decolouring: activated carbon decolorizing.
The 6th step, crystallization: 120-125 ℃ are concentrated into 70% above content, crystallisation by cooling.
The 7th step, separation, dry: separating machine is by crystal and liquid separation, and then fluidised bed drying, obtains Sunmorl N 60S finished product.
The 8th step, packing.
Claims (1)
1. Sunmorl N 60S high-efficiency fermenting production technique, comprising: the first step, and seed culture adds aspergillus niger strain and substratum to carry out seed culture in seed fermentation tank; Second step, fermentation, 30% Glucose Liquid and nutrition agent are added to fermentor tank sterilizing, cultured seed is added to fermentor tank in 20% ratio, total add-on is to 50% ~ 60% of fermentor tank working volume, exhaust analyzer or mass spectrograph that online examination of glucose concentration stream adds carbon dioxide and oxygen in controller and on-line analysis fermentation tail gas are installed on fermentor tank, meanwhile, the mass flowmeter of inlet and outlet air and the true microorganism cells volume of culture sensor of fermentor tank are also installed; The 3rd step, continuously ferments; The 4th step, filters Plate Filtration; The 5th step, decolouring, activated carbon decolorizing; The 6th step, crystallization, 120-125 ℃ is concentrated into 70% above content, crystallisation by cooling; The 7th step, separates, is dried, and separating machine is by crystal and liquid separation, and then fluidised bed drying, obtains Sunmorl N 60S finished product; The 8th step, packing; It is characterized in that: the 3rd step, continuously ferment, aforementioned second step operation is fermented latter stage, carry out on-line automatic 500g/L Glucose Liquid and the nutrition agent added according to the Sunmorl N 60S concentration of reduced sugar of on-line determination, discharge fermented liquid to surge tank from fermentor tank bottom simultaneously, also add NaOH(content 350g/L simultaneously); Be specially, when in fermented liquid when concentration of reduced sugar≤50g/L, automatically add Glucose Liquid and nutrition agent, discharge fermented liquid simultaneously and make concentration of reduced sugar maintain 30g/L-50g/L, and maintain certain bacterium dense (dry weight 10g/L is to 20g/L) and fermentating liquid volume; Fermentor tank cubic capacity is made as V, and unit is L, and the speed of adding Glucose Liquid and nutrition agent is S1, and the speed of adding NaOH is S2, and fermentation liquid draining speed is S, and maximum liquid amount is controlled at fermentor tank cubic capacity below 83%,
In fermentor tank, 180-220 hours mycelium of fermented liquid fermentation are aging, stop adding Glucose Liquid and nutrition agent, to fermentation ends; Be discharged to fermented liquid in surge tank by fermentor tank and continue to ventilate, stir, adding NaOH, that reducing sugar is utilized is complete.
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104805138A (en) * | 2015-04-07 | 2015-07-29 | 山东西王糖业有限公司 | Fermentation method of high-dry-matter sodium gluconate |
CN105255963A (en) * | 2015-11-11 | 2016-01-20 | 山东福洋生物科技有限公司 | Efficient fermentation control method for gel polysaccharide |
CN106282248A (en) * | 2016-08-08 | 2017-01-04 | 山东福洋生物科技有限公司 | A kind of continuous fermentation production method of sodium gluconate |
CN106337065A (en) * | 2016-08-24 | 2017-01-18 | 山东福洋生物科技有限公司 | Technological method for producing sodium gluconate by enzyme method |
CN106701846A (en) * | 2017-01-18 | 2017-05-24 | 华东理工大学 | Method for on-line monitoring and optimization of aspergillus niger fermentation production of sodium gluconate |
CN112244921A (en) * | 2020-11-11 | 2021-01-22 | 冯文红 | Anastomat |
CN116855550A (en) * | 2023-09-04 | 2023-10-10 | 山东福洋生物科技股份有限公司 | Method for producing sodium gluconate by enzyme method |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103352090A (en) * | 2013-06-18 | 2013-10-16 | 山东福洋生物科技有限公司 | Control method for efficient production of calcium gluconate by microbial fermentation |
-
2014
- 2014-04-10 CN CN201410140262.8A patent/CN103898171A/en active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103352090A (en) * | 2013-06-18 | 2013-10-16 | 山东福洋生物科技有限公司 | Control method for efficient production of calcium gluconate by microbial fermentation |
Non-Patent Citations (1)
Title |
---|
何国庆主编: "《食品发酵与酿造工艺学》", 31 December 2001, 中国农业出版社出版 * |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104805138A (en) * | 2015-04-07 | 2015-07-29 | 山东西王糖业有限公司 | Fermentation method of high-dry-matter sodium gluconate |
CN105255963A (en) * | 2015-11-11 | 2016-01-20 | 山东福洋生物科技有限公司 | Efficient fermentation control method for gel polysaccharide |
CN105255963B (en) * | 2015-11-11 | 2019-03-01 | 山东福洋生物科技有限公司 | Curdlan high-efficiency fermenting control method |
CN106282248A (en) * | 2016-08-08 | 2017-01-04 | 山东福洋生物科技有限公司 | A kind of continuous fermentation production method of sodium gluconate |
CN106337065A (en) * | 2016-08-24 | 2017-01-18 | 山东福洋生物科技有限公司 | Technological method for producing sodium gluconate by enzyme method |
CN106701846A (en) * | 2017-01-18 | 2017-05-24 | 华东理工大学 | Method for on-line monitoring and optimization of aspergillus niger fermentation production of sodium gluconate |
CN106701846B (en) * | 2017-01-18 | 2020-09-18 | 华东理工大学 | Method for producing sodium gluconate by on-line monitoring and optimizing aspergillus niger fermentation |
CN112244921A (en) * | 2020-11-11 | 2021-01-22 | 冯文红 | Anastomat |
CN112244921B (en) * | 2020-11-11 | 2023-08-04 | 冯文红 | Anastomat |
CN116855550A (en) * | 2023-09-04 | 2023-10-10 | 山东福洋生物科技股份有限公司 | Method for producing sodium gluconate by enzyme method |
CN116855550B (en) * | 2023-09-04 | 2023-11-17 | 山东福洋生物科技股份有限公司 | Method for producing sodium gluconate by enzyme method |
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