CN104805138A - Fermentation method of high-dry-matter sodium gluconate - Google Patents
Fermentation method of high-dry-matter sodium gluconate Download PDFInfo
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- CN104805138A CN104805138A CN201510160060.4A CN201510160060A CN104805138A CN 104805138 A CN104805138 A CN 104805138A CN 201510160060 A CN201510160060 A CN 201510160060A CN 104805138 A CN104805138 A CN 104805138A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
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Abstract
The invention belongs to the technical field of sodium gluconate production, and particularly relates to a fermentation method of high-dry-matter sodium gluconate. The fermentation method has the advantages that the growth potential of strains is sufficiently used, and the single tank productivity is improved by improving the dry matter of glucose. Particularly, the feeding manner of high-dry-matter glucose with the mass concentration of gluconate being 48-55% is adopted, the propionic acid output of a single tank is improved, the fermentation period is shortened, and the unit propionic acid output of a fermentation tank is improved.
Description
Technical field
The invention belongs to the technical field that Sunmorl N 60S is produced, be specially high dry value in ferment of sodium gluconate method.
Background technology
Current biological fermentation process has become the main production process of Sunmorl N 60S.The method is the glucose oxidase and the catalase that utilize Aspergillus Niger, and glucose dehydro is obtained Gluconolactone and hydrogen peroxide by glucose oxidase, then is hydrolyzed to gluconic acid further through catalase, and hydrogen peroxide is hydrolyzed into water and oxygen.Flow back to liquid caustic soda to keep certain pH value simultaneously, and obtain Sunmorl N 60S.In biological fermentation glucose process, in fermentation condition, glucose dry matter content affects the production capacity of fermentor tank, and in existing value in ferment of sodium gluconate process, glucose dry concentration is about 37%.
Value in ferment of sodium gluconate process is heat aerobic organisms fermentation, and dissolved oxygen is to most important in fermenting process.
Summary of the invention
The object of the invention is to for above-mentioned Problems existing and provide a kind of high dry value in ferment of sodium gluconate method, present method makes full use of the growth potential of bacterial classification, by improving the dry of glucose, improves single tank production capacity.Be specially the mode that employing glucose quality concentration is the high dry glucose feeding of 48-55%, improve the product acid amount of single tank, shorten fermentation period, improve fermentor tank unit product acid amount.
Technical scheme of the present invention is:
A kind of high dry value in ferment of sodium gluconate method, adopts the mode of high dry glucose feeding, improves the product acid amount of single tank.
The mass concentration that the stream that described high dry glucose refers to employing adds glucose is 48-55%.
This dry concentration 50-52% in the present invention, significantly improves compared with the glucose dry concentration of 37%, is referred to as " high dry " value in ferment of sodium gluconate.
Feature of the present invention also has:
High dry value in ferment of sodium gluconate method of the present invention, detailed step is: step (1) seed and stream add: the Glucose Liquid of sterilization is entered seeding tank, access bacterial classification, and first carry out seed culture, glucose concn is 250-320g/l; And then culture transferring is to fermentor tank, ventilating fermentation, detects mycelia situation in every 4 hours, produces acid amount and glucose residual quantity, and starting stream when mycelia reaches maximum product sour phase, to add mass concentration be 48-55% glucose;
Step (2) is fermented: after glucose feeding to fermentor tank, adjusts pH to 4.50-5.50 with NaOH, keeps temperature 29-31 DEG C, passes into 180-200m
3/ min pressurized air, adds potassium primary phosphate 1.0-1.2kg/T(material per ton and adds 1.0-1.2kg, lower same), primary ammonium phosphate 2.0-2.3kg/T, magnesium sulfate 0.8-1.0kg/T, residual sugar is below 0.2g/dl fermentation ends.
During the fermentation, the moment observes mycelial form, remains that mycelium is produced when glucose concn is 28.5-31.5g/dl, reduces high density produces acid impact on mycelium.
Further preferably, in step (1), the mass concentration that stream adds glucose is 50-52%.
Further preferably, in step (2), adjust pH to 5.0 with NaOH.
Further preferably, in step (2), potassium primary phosphate 1.1kg/T is added.
Further preferably, in step (2), primary ammonium phosphate 2.1kg/T.
Beneficial effect of the present invention is described below:
Shorten fermentation period: it is little that bacterial classification moves into fermentor grown environmental change from seeding tank, and the mycelia adaptive phase shortens.
Improve fermentor tank unit and produce acid amount: (mass concentration is 48-55% because stream adds the higher glucose of concentration, be preferably 50-52%, significantly improve than about 37% of existing value in ferment of sodium gluconate technique), totally can improve the dry of fermentation ends secondary fermentation liquid, can 53-55% be reached, namely improve the production capacity of fermentor tank.
Use rear effect comparison as follows:
Fermentor tank 280 cubes 7 covers, full fermentation period is 40 hours, and single tank puts tank volume 230 cubes, and fermented liquid concentration 30% calculates, and every day produces acid 327.47 tons.
Use the same fermentor tank of high concentration glucose feeding method fermentation method: fermentor tank 280 cubes 7 covers, fermentation period is 38 hours, and single tank puts tank volume 230 cubes, and fermented liquid concentration 32% calculates, and every day produces acid 370.94 tons, improves output 13.27%.
Embodiment
Below by specific embodiment, technical scheme of the present invention is described in detail.
In order to not affect mycelial growth in early stage, the present invention adopts the mode of high dry glucose feeding, improves the product acid amount of single tank.
embodiment 1
High dry value in ferment of sodium gluconate method of the present invention, detailed step is: step (1) seed and stream add: the Glucose Liquid of sterilization is entered seeding tank, access bacterial classification, and first carry out seed culture, glucose concn is 250-320g/l; And then culture transferring is to fermentor tank, ventilating fermentation, detects mycelia situation in every 4 hours, produces acid amount and glucose residual quantity, and starting stream when mycelia reaches maximum product sour phase, to add mass concentration be 50% glucose;
Step (2) is fermented: after glucose feeding to fermentor tank, adjusts pH to 5.0 with NaOH, keeps temperature 29-31 DEG C, passes into 180-200m
3/ min pressurized air, adds potassium primary phosphate 1.1kg/T(material per ton and adds 1.1kg, lower same), primary ammonium phosphate 2.1kg/T, magnesium sulfate 0.8-1.0kg/T, residual sugar is below 0.2g/dl fermentation ends.
During the fermentation, the moment observes mycelial form, remains that mycelium is produced when glucose concn is 28.5-31.5g/dl, reduces high density produces acid impact on mycelium.
Glucose concn 250-320g/l pressed by fermentor tank and seeding tank, after seed tank culture completes, culture transferring is to fermentor tank, because the growing environment of bacterial classification does not too much change, mycelia produces rapidly, within every 4 hours, detect mycelia situation, produce acid amount and glucose residual quantity, mycelia reaches the maximum product acid phase, start stream and increase dry glucose, suitable control flow check adds the amount of glucose, and budget list tank produces acid amount, moment observes mycelial form, remain that mycelium is produced when concentration is low, reduce high density produces acid impact on mycelium, according to budget situation, determine that stream adds the total amount of glucose.
embodiment 2
Starting stream when mycelia reaches maximum products sour phase in step (1), to add mass concentration be 52% glucose; All the other technical characteristics are with embodiment 1.
embodiment 3
Starting stream when mycelia reaches maximum products sour phase in step (1), to add mass concentration be 55% glucose;
Adjust pH to 4.5-5.0 with NaOH in step (2), all the other technical characteristics are with embodiment 1.
embodiment 4
Add potassium primary phosphate 1.0kg/T(material per ton in step (2) and add 1.0kg, lower same), primary ammonium phosphate 2.3kg/T, all the other technical characteristics are with embodiment 1.
embodiment 5
Add potassium primary phosphate 1.2kg/T(material per ton in step (2) and add 1.0kg, lower same), primary ammonium phosphate 2.0kg/T, all the other technical characteristics are with embodiment 1.
Claims (8)
1. a high dry value in ferment of sodium gluconate method, adopts the mode of high dry glucose feeding, improves the product acid amount of single tank.
2. high dry value in ferment of sodium gluconate method according to claim 1, is characterized in that, the mass concentration that the stream that described high dry glucose refers to employing adds glucose is 48-55%.
3. high dry value in ferment of sodium gluconate method according to claim 1, detailed step is: step (1) seed and stream add: the Glucose Liquid of sterilization is entered seeding tank, access bacterial classification, and first carry out seed culture, glucose concn is 250-320g/l; And then culture transferring is to fermentor tank, ventilating fermentation, detects mycelia situation in every 4 hours, produces acid amount and glucose residual quantity, and starting stream when mycelia reaches maximum product sour phase, to add mass concentration be 48-55% glucose;
Step (2) is fermented: after glucose feeding to fermentor tank, adjusts pH to 4.50-5.50 with NaOH, keeps temperature 29-31 DEG C, passes into 180-200m
3/ min pressurized air, adds potassium primary phosphate 1.0-1.2kg/T(material per ton and adds 1.0-1.2kg, lower same), primary ammonium phosphate 2.0-2.3kg/T, magnesium sulfate 0.8-1.0kg/T, residual sugar is below 0.2g/dl fermentation ends.
4. high dry value in ferment of sodium gluconate method according to claim 3, is characterized in that, during the fermentation, keeps mycelium to produce when glucose concn is 28.5-31.5g/dl.
5. high dry value in ferment of sodium gluconate method according to claim 3, is characterized in that, in step (1), the mass concentration that stream adds glucose is 50-52%.
6. high dry value in ferment of sodium gluconate method according to claim 3, is characterized in that, in step (2), adjusts pH to 5.0 with NaOH.
7. high dry value in ferment of sodium gluconate method according to claim 3, is characterized in that, in step (2), add potassium primary phosphate 1.1kg/T.
8. high dry value in ferment of sodium gluconate method according to claim 3, is characterized in that, in step (2), and primary ammonium phosphate 2.1kg/T.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101575621A (en) * | 2009-06-22 | 2009-11-11 | 江苏江山制药有限公司 | High concentration fermentation process of 2-keto-D-gluconic acid mixed salt |
| CN102296102A (en) * | 2011-08-18 | 2011-12-28 | 山东福洋生物科技有限公司 | Control method for gluconate production by microbiological method |
| CN102321689A (en) * | 2011-08-18 | 2012-01-18 | 山东福洋生物科技有限公司 | Semicontinuous fermentation technology of gluconate |
| CN103667375A (en) * | 2013-11-26 | 2014-03-26 | 郑州市中食农产品加工研究院 | Method for preparing sodium gluconate by adopting aspergillus niger fermentation method |
| CN103898171A (en) * | 2014-04-10 | 2014-07-02 | 山东福洋生物科技有限公司 | Efficient fermentation production process of sodium gluconate |
-
2015
- 2015-04-07 CN CN201510160060.4A patent/CN104805138A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101575621A (en) * | 2009-06-22 | 2009-11-11 | 江苏江山制药有限公司 | High concentration fermentation process of 2-keto-D-gluconic acid mixed salt |
| CN102296102A (en) * | 2011-08-18 | 2011-12-28 | 山东福洋生物科技有限公司 | Control method for gluconate production by microbiological method |
| CN102321689A (en) * | 2011-08-18 | 2012-01-18 | 山东福洋生物科技有限公司 | Semicontinuous fermentation technology of gluconate |
| CN103667375A (en) * | 2013-11-26 | 2014-03-26 | 郑州市中食农产品加工研究院 | Method for preparing sodium gluconate by adopting aspergillus niger fermentation method |
| CN103898171A (en) * | 2014-04-10 | 2014-07-02 | 山东福洋生物科技有限公司 | Efficient fermentation production process of sodium gluconate |
Non-Patent Citations (2)
| Title |
|---|
| 孙俊良: "《食品生物技术》", 31 October 2011 * |
| 陈骿声主编: "《有机酸发酵生产技术》", 31 January 1991 * |
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Application publication date: 20150729 |