CN103667375A - Method for preparing sodium gluconate by adopting aspergillus niger fermentation method - Google Patents
Method for preparing sodium gluconate by adopting aspergillus niger fermentation method Download PDFInfo
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- CN103667375A CN103667375A CN201310610412.2A CN201310610412A CN103667375A CN 103667375 A CN103667375 A CN 103667375A CN 201310610412 A CN201310610412 A CN 201310610412A CN 103667375 A CN103667375 A CN 103667375A
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Abstract
The invention provides a method for preparing sodium gluconate by adopting an aspergillus niger fermentation method. The method comprises the following steps: (1) refining sugar: using starch such as corn, wheat, rice, sweet potato, potato as raw materials, preparing starch milk, and obtaining saccharification liquid containing glucose through liquification and a saccharify enzyme hydrolysis; (2) fermenting: sending the saccharification liquid into a batching tank through actived carbon decoloring, sheet frame filtering and Pe macromolecule microporous filter filtering, adding auxiliary materials such as NH4H2PO4, KH2PO4, MgSO4.7H2O as a fermentation medium, performing continuous sterilization, and inoculating aspergillus niger to ferment; (3) extracting: performing decoloring, sheet frame filtering and Pe macromolecule microporous filter filtering on the fermented sodium gluconate solution, centrifugally separating supernatant, performing vacuum concentration, stewing, crystallizing, centrifugally separating again so as to obtain sodium gluconate crystal, and drying so as to obtain the sodium gluconate product. The sodium gluconate prepared by adopting the aspergillus niger fermentation method has no heavy metal residual, has no waste water and waste gas, ensures the food safety, and is simple in technology, easy to control and relatively low in cost.
Description
Technical field
The present invention relates to the preparation method of Sunmorl N 60S, particularly adopt fermentation of Aspergillus niger legal system for the method for Sunmorl N 60S.
Background technology
Sunmorl N 60S claims again sodium pentahydyoxycaproate, is a kind of white or light yellow crystalline powder, and the alcohol that is slightly soluble in soluble in water, is insoluble to ether.Sunmorl N 60S is the deep processed product of glucose, is also the basic material of preparing Gluconolactone, gluconate (zinc, copper, ferrous salt) etc., in foodstuffs industry, can be used as accessory substance, solidifying agent, buffer reagent etc.
The turnout of domestic Sunmorl N 60S constantly increased in recent years, and these producers mostly adopt chemical method synthesis of glucose acid sodium, as eletrooxidation method, and electrolytic oxidation, heterogeneous catalytic oxidation method etc.Domestic many producers pay little attention to Sunmorl N 60S quality product, devote exclusive attention to output simply, and product ubiquity water content is high, granularity is less, color and luster and the problem such as product purity is inadequate.Therefore, domestic a large amount of Sunmorl N 60S products are mainly used in building, water treatment and cleaning industry, less for the product of food and medicine.
Chemical process produce Sunmorl N 60S be generally faced with quality wayward, consume energy large, seriously polluted, and in Sunmorl N 60S product, likely have heavy metal contamination, these drawbacks, make that enterprise's production cost is high, product does not reach food safety and medical standard.
Summary of the invention
For the technical problem existing in prior art, the invention provides a kind of fermentation of Aspergillus niger legal system that adopts for the method for Sunmorl N 60S, can avoid introducing in product heavy metal, guarantee food safety, and it is low to have cost, system easy to control the quality, without the advantage of waste water, waste gas.
For solving the problems of the technologies described above, the present invention realizes with following technical proposals: the present invention adopts fermentation of Aspergillus niger legal system for the method for Sunmorl N 60S, comprise the following steps: (1) sugaring: with (corn, wheat, rice, Ipomoea batatas, potato) starch is raw material, preparation 24-26%(butt starch w/%) starch milk, adjust pH 6.2-6.4, add high temperature resistant α-amylase 12-14U/g, add a small amount of CaCl2 simultaneously, consumption is the 0.1-0.3% of dry starch, steam ejection liquefaction, charging pump pressure 0.4-0.5MPa, injection temperature 100-105 ℃, maintain tank temperature 95-105 ℃, 25-30min holds time, reduce by 60 ± 2 ℃ of temperature, adjust pH4.5-4.8, add saccharifying enzyme 100-300U/g, continuously stirring 20-25h, through double-enzyme method, be hydrolyzed into glucose, after filtration, become saccharified liquid, (2) fermentation: saccharified liquid adopts activated carbon decolorizing, activated carbon dosage 2-4%, temperature 80-85 ℃, time 20-25min, pH4.4-4.8, decolouring is by Plate Filtration, remove powdered carbons more than 10 μ m, through Pe high molecular millipore filter device, remove the following activated carbon granule of 10 μ m again, then send into material-compound tank, add auxiliary material NH4H2PO40.5-0.8g/L, KH2PO40.2-0.5g/L, MgSO47H2O0.2-0.5g/L, through continuous sterilization, 115 ℃ maintain 20-30min, lower the temperature 30 ± 2 ℃ and enter fermentor tank, and then access aspergillus niger (our unit's laboratory screening obtains) ferments, fermented liquid is 1:1-1.5 with passing into sterile air amount, 30 ± 2 ℃ of leavening temperatures, stirring velocity 250-300r/min, pH5.8-6.2, (3) extract: the sodium gluconate solution that second step fermentation is obtained decolours, 80 ± 2 ℃ of bleaching temperatures, activated carbon dosage 0.4-0.6%, bleaching time 30-35min, decolouring is filtered removal gac and black-koji mould filament by Plate Filtration and Pe high molecular millipore filter device, centrifugal (3000-4000r/min) separation obtains supernatant liquor, supernatant liquor vacuum concentration is to concentration 60-80%, standing crystallisation by cooling 4-6h, centrifugal (3000-4000r/min) obtains gluconic acid sodium crystal, supernatant liquor turns back in condensing crystal tank, gluconic acid sodium crystal is dried under 60-80 ℃ of condition.
The present invention is with respect to prior art, can avoid introducing heavy metal in product, guarantee food safety, and quality product is easy to control, in production process without waste water, waste gas; The present invention utilizes microbial immobilized technology, by optimizing fermenting process, finds the mode of cheap carbon source, not only reduces production costs, and has also improved production efficiency simultaneously.
Accompanying drawing explanation
Fig. 1 is process flow sheet of the present invention.
embodiment
Embodiment 1: as shown in Figure 1, a kind of fermentation of Aspergillus niger legal system that adopts is for the method for Sunmorl N 60S, comprise the following steps: (1) sugaring: take W-Gum as raw material, preparation 25%(butt starch w/%) starch milk, adjust pH 6.2, add high temperature resistant α-amylase 12U/g, add a small amount of CaCl2 simultaneously, consumption is 0.1% of dry starch, steam ejection liquefaction, charging pump pressure 0.4MPa, 100 ℃ of injection temperatures, maintain 95 ℃ of tank temperature, 30min holds time, reduce temperature 60 C, adjust pH4.5, add saccharifying enzyme 200U/g, continuously stirring 25h, through double-enzyme method, be hydrolyzed into glucose, after filtration, become saccharified liquid, (2) fermentation: saccharified liquid adopts activated carbon decolorizing, activated carbon dosage 2%, 80 ℃ of temperature, time 20min, pH4.5, decolouring is by Plate Filtration, remove powdered carbons more than 10 μ m, through Pe high molecular millipore filter device, remove the following activated carbon granule of 10 μ m again, then send into material-compound tank, add auxiliary material NH4H2PO40.5g/L, KH2PO40.4g/L, MgSO47H2O0.4g/L, through continuous sterilization, 115 ℃ maintain 30min, lower the temperature 30 ℃ and enter fermentor tank, and then access aspergillus niger (our unit's laboratory screening obtains) ferments, fermented liquid is 1:1-1.5 with passing into sterile air amount, 30 ℃ of leavening temperatures, stirring velocity 250r/min, pH5.8, (3) extract: the sodium gluconate solution that second step fermentation is obtained decolours, 80 ℃ of bleaching temperatures, activated carbon dosage 0.5%, bleaching time 30min, decolouring is filtered removal gac and black-koji mould filament by Plate Filtration and Pe high molecular millipore filter device, centrifugal (3500r/min) separation obtains supernatant liquor, supernatant liquor vacuum concentration is to concentration 65%, standing crystallisation by cooling 6h, centrifugal (3500r/min) obtains gluconic acid sodium crystal, supernatant liquor turns back in condensing crystal tank, gluconic acid sodium crystal is dried under 60 ℃ of conditions, now Sunmorl N 60S productive rate can reach 91.5%.
Embodiment 2: as shown in Figure 1, a kind of fermentation of Aspergillus niger legal system that adopts is for the method for Sunmorl N 60S, comprise the following steps: (1) sugaring: take W-Gum as raw material, preparation 25%(butt starch w/%) starch milk, adjust pH 6.2, add high temperature resistant α-amylase 12U/g, add a small amount of CaCl2 simultaneously, consumption is 0.2% of dry starch, steam ejection liquefaction, charging pump pressure 0.4MPa, 105 ℃ of injection temperatures, maintain 100 ℃ of tank temperature, 30min holds time, reduce temperature 60 C, adjust pH4.5, add saccharifying enzyme 300U/g, continuously stirring 25h, through double-enzyme method, be hydrolyzed into glucose, after filtration, become saccharified liquid, (2) fermentation: saccharified liquid adopts activated carbon decolorizing, activated carbon dosage 3%, 85 ℃ of temperature, time 20min, pH4.6, decolouring is by Plate Filtration, remove powdered carbons more than 10 μ m, through Pe high molecular millipore filter device, remove the following activated carbon granule of 10 μ m again, then send into material-compound tank, add auxiliary material NH4H2PO40.8g/L, KH2PO40.4g/L, MgSO47H2O0.4g/L, through continuous sterilization, 115 ℃ maintain 30min, lower the temperature 30 ℃ and enter fermentor tank, and then access aspergillus niger (our unit's laboratory screening obtains) ferments, fermented liquid is 1:1-1.5 with passing into sterile air amount, 30 ℃ of leavening temperatures, stirring velocity 260r/min, pH5.8, (3) extract: the sodium gluconate solution that second step fermentation is obtained decolours, 80 ℃ of bleaching temperatures, activated carbon dosage 0.5%, bleaching time 30min, decolouring is filtered removal gac and black-koji mould filament by Plate Filtration and Pe high molecular millipore filter device, centrifugal (3500r/min) separation obtains supernatant liquor, supernatant liquor vacuum concentration is to concentration 70%, standing crystallisation by cooling 6h, centrifugal (3500r/min) obtains gluconic acid sodium crystal, supernatant liquor turns back in condensing crystal tank, gluconic acid sodium crystal is dried under 60 ℃ of conditions, now Sunmorl N 60S productive rate can reach 93.8%.
Embodiment 3: as shown in Figure 1, a kind of fermentation of Aspergillus niger legal system that adopts is for the method for Sunmorl N 60S, comprise the following steps: (1) sugaring: take W-Gum as raw material, preparation 25%(butt starch w/%) starch milk, adjust pH 6.4, add high temperature resistant α-amylase 14U/g, add a small amount of CaCl2 simultaneously, consumption is 0.3% of dry starch, steam ejection liquefaction, charging pump pressure 0.5MPa, 105 ℃ of injection temperatures, maintain 100 ℃ of tank temperature, 30min holds time, reduce temperature 60 C, adjust pH4.8, add saccharifying enzyme 300U/g, continuously stirring 25h, through double-enzyme method, be hydrolyzed into glucose, after filtration, become saccharified liquid, (2) fermentation: saccharified liquid adopts activated carbon decolorizing, activated carbon dosage 3%, 85 ℃ of temperature, time 20min, pH4.6, decolouring is by Plate Filtration, remove powdered carbons more than 10 μ m, through Pe high molecular millipore filter device, remove the following activated carbon granule of 10 μ m again, then send into material-compound tank, add auxiliary material NH4H2PO40.8g/L, KH2PO40.5g/L, MgSO47H2O0.5g/L, through continuous sterilization, 115 ℃ maintain 30min, lower the temperature 30 ℃ and enter fermentor tank, and then access aspergillus niger (our unit's laboratory screening obtains) ferments, fermented liquid is 1:1-1.5 with passing into sterile air amount, 32 ℃ of leavening temperatures, stirring velocity 270r/min, pH6.0, (3) extract: the sodium gluconate solution that second step fermentation is obtained decolours, 80 ℃ of bleaching temperatures, activated carbon dosage 0.5%, bleaching time 30min, decolouring is filtered removal gac and black-koji mould filament by Plate Filtration and Pe high molecular millipore filter device, centrifugal (3500r/min) separation obtains supernatant liquor, supernatant liquor vacuum concentration is to concentration 75%, standing crystallisation by cooling 6h, centrifugal (3500r/min) obtains gluconic acid sodium crystal, supernatant liquor turns back in condensing crystal tank, gluconic acid sodium crystal is dried under 60 ℃ of conditions, now Sunmorl N 60S productive rate can reach 95.2%.
Claims (10)
1. one kind adopts fermentation of Aspergillus niger legal system for the method for Sunmorl N 60S, it is characterized in that comprising the following steps: (1) sugaring: (corn, wheat, rice, Ipomoea batatas, the potato) starch of take is raw material, preparation 24-26%(butt starch w/%) starch milk, adjust pH 6.2-6.4, add high temperature resistant α-amylase 12-14U/g, add a small amount of CaCl simultaneously
2, the 0.1-0.3% that consumption is dry starch, steam ejection liquefaction, charging pump pressure 0.4-0.5MPa, injection temperature 100-105 ℃, maintains tank temperature 95-105 ℃, and 25-30min holds time, reduce by 60 ± 2 ℃ of temperature, adjust pH4.5-4.8, add saccharifying enzyme 100-300U/g (starch butt relatively), continuously stirring 20-25h, through double-enzyme method, be hydrolyzed into glucose, after filtration, become saccharified liquid, (2) fermentation: saccharified liquid adopts activated carbon decolorizing, activated carbon dosage 2-4%, temperature 80-85 ℃, time 20-25min, pH4.4-4.8, decolouring is by Plate Filtration, remove powdered carbons more than 10 μ m, through Pe high molecular millipore filter device, remove the following activated carbon granule of 10 μ m again, then send into material-compound tank, add auxiliary material NH
4h
2pO
40.5-0.8g/L, KH
2pO
40.2-0.5g/L, MgSO
47H
2o 0.2-0.5g/L, through continuous sterilization, 115 ℃ maintain 20-30min, lower the temperature 30 ± 2 ℃ and enter fermentor tank, and then access aspergillus niger (our unit's laboratory screening obtains) ferments, fermented liquid is with to pass into sterile air amount be 1:1-1.5,30 ± 2 ℃ of leavening temperatures, stirring velocity 250-300r/min, pH5.8-6.2, (3) extract: the sodium gluconate solution that second step fermentation is obtained decolours, 80 ± 2 ℃ of bleaching temperatures, activated carbon dosage 0.4-0.6%, bleaching time 30-35min, decolouring is filtered removal gac and black-koji mould filament by Plate Filtration and Pe high molecular millipore filter device, centrifugal (3000-4000r/min) separation obtains supernatant liquor, supernatant liquor vacuum concentration is to concentration 60-80%, standing crystallisation by cooling 4-6h, centrifugal (3000-4000r/min) obtains gluconic acid sodium crystal, supernatant liquor turns back in condensing crystal tank, gluconic acid sodium crystal is dried under 60-80 ℃ of condition.
2. according to the preparation method described in claim 1, it is characterized in that, described starch is one or more in W-Gum, wheat starch, Starch rice, sweet potato starch, potato starch.
3. according to the preparation method described in claim 1, it is characterized in that described saccharified liquid activated carbon decolorizing, activated carbon dosage 2-4%, temperature 80-85 ℃, time 20-25min, pH4.4-4.8.
4. according to the preparation method described in claim 1, it is characterized in that, after described saccharified liquid decolouring, through Plate Filtration, remove powdered carbons more than 10 μ m, then remove the following activated carbon granule of 10 μ m through Pe high molecular millipore filter device.
5. according to the preparation method described in claim 1, it is characterized in that, described fermentation adds auxiliary material NH
4h
2pO
40.5-0.8g/L, KH
2pO
40.2-0.5g/L, MgSO
47H
2o 0.2-0.5g/L.
6. according to the preparation method described in claim 1, it is characterized in that, described bacterial classification is that aspergillus niger is our unit's screening gained.
7. according to the preparation method described in claim 1, it is characterized in that, described fermentation condition is that fermented liquid is 1:1-1.5 with passing into sterile air amount, 30 ± 2 ℃ of leavening temperatures, stirring velocity 250-300r/min, pH5.8-6.2.
8. according to the preparation method described in claim 1, it is characterized in that, the sodium gluconate solution that in described abstraction process, fermentation obtains decolours, 80 ± 2 ℃ of bleaching temperatures, activated carbon dosage 0.4-0.6%, bleaching time 30-35min.
9. according to the preparation method described in claim 1, it is characterized in that, in described abstraction process, sodium gluconate solution decolouring is filtered removal gac and black-koji mould filament by Plate Filtration and Pe high molecular millipore filter device, and centrifugal (3000-4000r/min) separation obtains supernatant liquor.
10. according to the preparation method described in claim 1, it is characterized in that, described crystallization condition be supernatant liquor vacuum concentration to concentration 60-80%, standing crystallisation by cooling 4-6h.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103981227A (en) * | 2014-05-07 | 2014-08-13 | 内蒙古玉王生物科技开发有限公司 | Production method for food-grade sodium gluconate |
| CN104805138A (en) * | 2015-04-07 | 2015-07-29 | 山东西王糖业有限公司 | Fermentation method of high-dry-matter sodium gluconate |
| CN104830918A (en) * | 2015-04-07 | 2015-08-12 | 山东西王糖业有限公司 | Novel production method of sodium gluconate |
| CN105002226A (en) * | 2015-06-25 | 2015-10-28 | 山东省食品发酵工业研究设计院 | Method for sterilization of medium for production of sodium gluconate by Aspergillus niger fermentation |
| CN106337066A (en) * | 2016-08-31 | 2017-01-18 | 诸城东晓生物科技有限公司 | Energy-saving and environment-friendly enzymatic sodium gluconate producing new process |
| CN106701846A (en) * | 2017-01-18 | 2017-05-24 | 华东理工大学 | Method for on-line monitoring and optimization of aspergillus niger fermentation production of sodium gluconate |
| CN106778055A (en) * | 2016-11-29 | 2017-05-31 | 华东理工大学 | The modeling method of value in ferment of sodium gluconate industrial device model |
| CN106868065A (en) * | 2017-03-31 | 2017-06-20 | 山东福洋生物科技有限公司 | The continuous producing method and device of a kind of sodium gluconate |
| CN105002226B (en) * | 2015-06-25 | 2018-08-31 | 山东省食品发酵工业研究设计院 | A method of being applied to fermentation of Aspergillus niger and produces sodium gluconate medium sterilization |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103981227A (en) * | 2014-05-07 | 2014-08-13 | 内蒙古玉王生物科技开发有限公司 | Production method for food-grade sodium gluconate |
| CN104805138A (en) * | 2015-04-07 | 2015-07-29 | 山东西王糖业有限公司 | Fermentation method of high-dry-matter sodium gluconate |
| CN104830918A (en) * | 2015-04-07 | 2015-08-12 | 山东西王糖业有限公司 | Novel production method of sodium gluconate |
| CN105002226A (en) * | 2015-06-25 | 2015-10-28 | 山东省食品发酵工业研究设计院 | Method for sterilization of medium for production of sodium gluconate by Aspergillus niger fermentation |
| CN105002226B (en) * | 2015-06-25 | 2018-08-31 | 山东省食品发酵工业研究设计院 | A method of being applied to fermentation of Aspergillus niger and produces sodium gluconate medium sterilization |
| CN106337066A (en) * | 2016-08-31 | 2017-01-18 | 诸城东晓生物科技有限公司 | Energy-saving and environment-friendly enzymatic sodium gluconate producing new process |
| CN106778055A (en) * | 2016-11-29 | 2017-05-31 | 华东理工大学 | The modeling method of value in ferment of sodium gluconate industrial device model |
| CN106701846A (en) * | 2017-01-18 | 2017-05-24 | 华东理工大学 | Method for on-line monitoring and optimization of aspergillus niger fermentation production of sodium gluconate |
| CN106701846B (en) * | 2017-01-18 | 2020-09-18 | 华东理工大学 | Method for producing sodium gluconate by on-line monitoring and optimizing aspergillus niger fermentation |
| CN106868065A (en) * | 2017-03-31 | 2017-06-20 | 山东福洋生物科技有限公司 | The continuous producing method and device of a kind of sodium gluconate |
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Application publication date: 20140326 |