CN106337066A - Energy-saving and environment-friendly enzymatic sodium gluconate producing new process - Google Patents
Energy-saving and environment-friendly enzymatic sodium gluconate producing new process Download PDFInfo
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- CN106337066A CN106337066A CN201610780520.8A CN201610780520A CN106337066A CN 106337066 A CN106337066 A CN 106337066A CN 201610780520 A CN201610780520 A CN 201610780520A CN 106337066 A CN106337066 A CN 106337066A
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- sodium gluconate
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- heat exchanger
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- AEQDJSLRWYMAQI-UHFFFAOYSA-N 2,3,9,10-tetramethoxy-6,8,13,13a-tetrahydro-5H-isoquinolino[2,1-b]isoquinoline Chemical compound C1CN2CC(C(=C(OC)C=C3)OC)=C3CC2C2=C1C=C(OC)C(OC)=C2 AEQDJSLRWYMAQI-UHFFFAOYSA-N 0.000 title claims abstract description 40
- 239000000176 sodium gluconate Substances 0.000 title claims abstract description 40
- 229940005574 sodium gluconate Drugs 0.000 title claims abstract description 40
- 235000012207 sodium gluconate Nutrition 0.000 title claims abstract description 40
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 8
- 238000000034 method Methods 0.000 title abstract description 23
- 230000008569 process Effects 0.000 title abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 92
- 238000000855 fermentation Methods 0.000 claims abstract description 51
- 230000004151 fermentation Effects 0.000 claims abstract description 51
- 229920002472 Starch Polymers 0.000 claims abstract description 30
- 235000019698 starch Nutrition 0.000 claims abstract description 30
- 239000008107 starch Substances 0.000 claims abstract description 30
- 238000004519 manufacturing process Methods 0.000 claims abstract description 25
- 238000001035 drying Methods 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000005406 washing Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 102000004190 Enzymes Human genes 0.000 claims description 27
- 108090000790 Enzymes Proteins 0.000 claims description 27
- 229940088598 enzyme Drugs 0.000 claims description 27
- 238000005516 engineering process Methods 0.000 claims description 18
- 238000007701 flash-distillation Methods 0.000 claims description 17
- 235000013336 milk Nutrition 0.000 claims description 17
- 239000008267 milk Substances 0.000 claims description 17
- 210000004080 milk Anatomy 0.000 claims description 17
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 15
- 108090000637 alpha-Amylases Proteins 0.000 claims description 11
- 238000001816 cooling Methods 0.000 claims description 11
- 238000001704 evaporation Methods 0.000 claims description 11
- 230000008020 evaporation Effects 0.000 claims description 11
- 238000000746 purification Methods 0.000 claims description 9
- 102000004139 alpha-Amylases Human genes 0.000 claims description 8
- 229940024171 alpha-amylase Drugs 0.000 claims description 8
- 210000000481 breast Anatomy 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000004366 Glucose oxidase Substances 0.000 claims description 6
- 108010015776 Glucose oxidase Proteins 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 229940116332 glucose oxidase Drugs 0.000 claims description 6
- 235000019420 glucose oxidase Nutrition 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 238000009423 ventilation Methods 0.000 claims description 6
- 238000010792 warming Methods 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000002918 waste heat Substances 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 238000009210 therapy by ultrasound Methods 0.000 claims description 4
- 239000006200 vaporizer Substances 0.000 claims description 4
- 241000228245 Aspergillus niger Species 0.000 claims description 3
- 102000016938 Catalase Human genes 0.000 claims description 3
- 108010053835 Catalase Proteins 0.000 claims description 3
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims description 3
- 241000228143 Penicillium Species 0.000 claims description 3
- 239000002202 Polyethylene glycol Substances 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 3
- 108010019077 beta-Amylase Proteins 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 239000013530 defoamer Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 3
- 229910052738 indium Inorganic materials 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229940038504 oxygen 100 % Drugs 0.000 claims description 3
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 3
- 239000008363 phosphate buffer Substances 0.000 claims description 3
- 229920001223 polyethylene glycol Polymers 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000002525 ultrasonication Methods 0.000 claims description 3
- 238000002604 ultrasonography Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 239000013049 sediment Substances 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000003197 catalytic effect Effects 0.000 abstract description 4
- 230000001804 emulsifying effect Effects 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 238000007670 refining Methods 0.000 abstract 1
- NBGBEUITCPENLJ-UHFFFAOYSA-N Bunazosin hydrochloride Chemical compound Cl.C1CN(C(=O)CCC)CCCN1C1=NC(N)=C(C=C(OC)C(OC)=C2)C2=N1 NBGBEUITCPENLJ-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000009923 sugaring Methods 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- IDAGXRIGDWCIET-SDFKWCIISA-L disodium;(2s,3s,4s,5r)-2,3,4,5-tetrahydroxyhexanedioate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O IDAGXRIGDWCIET-SDFKWCIISA-L 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000007210 heterogeneous catalysis Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000011344 liquid material Substances 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- UPMFZISCCZSDND-JJKGCWMISA-M sodium gluconate Chemical compound [Na+].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O UPMFZISCCZSDND-JJKGCWMISA-M 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
Abstract
The invention belongs to the technical field of sodium gluconate production, and particularly relates to an energy-saving and environment-friendly enzymatic sodium gluconate producing new process. The energy-saving and environment-friendly enzymatic sodium gluconate producing new process comprises the following steps: (1) emulsifying and saccharifying starch, comprising a saccharifying substep c; (2) continuously digesting; (3) fermenting, comprising a saccharified liquid fermenting substep b; (4) decolorizing; (5) concentrating; (6) filtering, washing, and drying to obtain a finished product. Compared with the conventional catalytic method and the conventional fermentation method, the energy-saving and environment-friendly enzymatic sodium gluconate producing new process has the remarkable advantages of high safety, and easy extraction and refining of the product; by the energy-saving and environment-friendly enzymatic sodium gluconate producing new process, the quality of sodium gluconate is significantly improved, and the steam consumption of the product can be reduced, so that the production cost of the product is significantly reduced.
Description
Technical field
The invention belongs to the technical field that sodium gluconate produces, it is specifically related to a kind of energy saving and environment friendly Production by Enzymes Portugal
Grape sodium saccharate new technology.
Background technology
Sodium gluconate also known as sodium pentahydyoxycaproate, molecular formula is c6h11o7na.Due to the nontoxic, spy such as heat stability is good
Point, has a wide range of applications at aspects such as building industry, food, medicine.Because sodium gluconate has important purposes, and
In recent years, with food industry, the developing rapidly of building trade, the demand of China's sodium gluconate is increasing, therefore,
The attention such as the production technology of sodium gluconate and technical study improvement.
The production method of sodium gluconate mainly has eletrooxidation method, electrolytic oxidation, heterogeneous catalytic oxidation at present
Method and biological fermentation process.Wherein now it is most commonly used that heterogeneous catalytic oxidation method and biological fermentation process.Heterogeneous catalytic oxidation method is subject to
To the restriction of catalyst efficiency, production cost is higher.Further, since heavy metal has certain toxicity, heterogeneous catalysiss method
The sodium gluconate producing should not be applied to field of food, and application range of products is restricted；Fermentation method has the easy microbiological contamination of strain,
In fermentation liquid, soluble protein is difficult removes, the defect such as operating procedure is many, limits the yield of sodium gluconate product, color and pure
The raising of degree.
The energy consumption of prior art sodium gluconate production process is higher simultaneously, energy utilization rate relatively low it is impossible to meet energy-conservation
The demand of environmental protection.
Content of the invention
Present invention aims to above-mentioned defect and a kind of energy saving and environment friendly Production by Enzymes glucose is provided
Sour sodium new technology, this technique has safe compared to traditional catalysis method and fermentation method, and product is easy to extract and refined
Significant advantage, increases substantially the quality of sodium gluconate, can reduce the steam consumption of product so that product cost simultaneously
It is greatly lowered.
The technical scheme is that a kind of energy saving and environment friendly Production by Enzymes sodium gluconate new technology, walk including following
Rapid: the liquefaction of (1) starch milk, saccharifying: a. liquefaction: to adopt 50-60 DEG C of high-temperature-hot-water first in plate type heat exchanger to raw starch
Breast carries out heat exchange and is heated up to 40-45 DEG C, then raw starch breast is sized mixing to mass fraction as being delivered to liquefaction spray after 40-44%
In emitter, adjusting ph value is 4.8-5.0, is successively sequentially added into 11-13u/g Thermostable α-Amylase and mass fraction is
0.05-0.08% anhydrous calcium chloride carries out the 15-24 minute that liquefies at 80-95 DEG C, simultaneously at ultrasound wave under the conditions of power 240w
Reason 6-10 minute；Add 12-15u/g Thermostable α-Amylase to carry out liquefaction 12-16 at finally adjusting the temperature to 100-110 DEG C to divide
Clock obtains liquefier；
B. flash distillation, cooling: 100-110 DEG C of liquefier entrance flash tank of gained carries out flash distillation and respectively obtain secondary flash vapour and 95-
99 DEG C of flash distillation liquefiers；95-99 DEG C of flash distillation liquefier first passes around plate type heat exchanger heat exchange and is cooled to 80-85 DEG C, Ran Houzai
It is cooled to 58-62 DEG C via plate type heat exchanger heat exchange；
C. saccharifying: 58-62 DEG C of flash distillation liquefier of gained is delivered in saccharifying tank, adjusting ph value at 60 DEG C of constant temperature is 4.0-
4.2, successively add 0.331ausp pullulanase, 218.1u/g beta amylase and 51.32u/g amyloglucosidase successively, enter
Row enzymatic saccharification 3.5-5.5 hour, obtains 58-62 DEG C of saccharified liquid, this saccharified liquid is warming up to 75- through plate type heat exchanger heat exchange
80 DEG C, wherein contained by gained saccharified liquid, glucose quality fraction is 35%-37%；
(2) even disappear: 75-80 DEG C of saccharified liquid of gained being delivered to fermentation even disappear and carry out even disappearing in ejector, the sugar after even disappearing
Change liquid carries out heat exchange through plate type heat exchanger and is cooled to 37-39 DEG C；
(3) ferment: a. enzyme preparation a: penicillium sp and aspergillus niger are placed in container than 1.5:1 according to weight, by every gram of mycelia
The ratio of 3.0ml, adds the phosphate buffer of ph5.1, freezes 15 minutes, room temperature is melted, repeatedly twice at being positioned over -25 DEG C；
Then the purification of ethanol being 70% using volume fraction after ultrasonication 5-8 minute under ultrasonic power 400w, using quality
Fraction is that 14% Polyethylene Glycol secondarily purified obtains purification glucoseoxidase liquid；Purification glucoseoxidase liquid adds
Catalase obtains double enzyme liquids, and wherein catalatic is 0.05g/ml to enzyme amount；Add after taking hydroxyapatite sterilizing
To double enzyme liquids, wherein solid-to-liquid ratio is 1:10；Stand 2-3.5 hour at 3 DEG C, be centrifuged under the conditions of 3500r after natural subsidence
So that impregnated carrier is separated with clear liquid within 10 minutes, obtain impregnated carrier；
B. saccharified liquid fermentation: saccharified liquid is added in fermentation tank, is incubated 38-39 DEG C, is passed through oxygen 0.012m3/ min, rotating speed
100r/min, treats dissolved oxygen stable post-equalization dissolved oxygen 100%；Stop ventilation, pressure release, add impregnated carrier, wherein solid-to-liquid ratio is 1:
20；Recover ventilation, keep tank pressure 0.15 mpa, add defoamer 0.3 ml, keep ph value to be 4.5-5.0, control temperature to exist
38-39 DEG C carries out fermentation and obtains fermentation liquid；38-39 DEG C of fermentation liquid is warming up to 45-50 DEG C through plate type heat exchanger heat exchange；
(4) decolour: using Powdered Activated Carbon ak-220 in temperature 45-50 DEG C, ph value is sending out after heat exchanging under the conditions of 4.5-5.2
Zymotic fluid decolouring 20-35 minute；
(5) concentrate: the fermentation liquid after decolouring enters vaporizer and is evaporated concentrating, and wherein fermentation liquid first passes around evaporation preheater
It is preheated to 60-75 DEG C, evaporation preheater adopts secondary flash vapour waste heat；
(6) filtration, washing, drying, finished product.
In described step (1), the high-temperature-hot-water of plate type heat exchanger is by plate type heat exchanger liquefier heat exchange temperature raising medium water
With plate type heat exchanger saccharified liquid heat exchange temperature raising medium water composition.
In described step (1), the heat exchange low temperature water outlet of plate type heat exchanger is entered in plate type heat exchanger and is carried out with fermentation liquid
Heat exchange.
The solid-to-liquid ratio of the fermentation liquid after Powdered Activated Carbon ak-220 and heat exchange in the decolouring of described step (4) is 1:15.
In described step (1), the temperature of secondary flash vapour is more than 100 DEG C.
In described step (1), ultrasonic Treatment is specially and for ultrasonic variable amplitude bar to insert below starch milk liquid level 2.5-3
Cm, magnetic agitation rotating speed 50%.
The invention has the benefit that energy saving and environment friendly Production by Enzymes sodium gluconate new technology of the present invention is with starch
Breast is material, enzyme method production sodium gluconate, process reagents, ginseng in cooperation between each step for this new technology and each step
The collocation of number and condition has carried out the collocation design of scientific rationalization so that the yield of sodium gluconate improves 37.3-
43.8%.It is specifically described below as follows:
(1) liquefaction sizes mixing raw starch breast to mass fraction is 40-50%, improves starch concentration, reduces in production process
The energy and the consumption of water, through production practices, the starch milk of this concentration makes energy consumption in sodium gluconate production process reduce
23.7-26.1%, water consumption decreases 34.8%-41.6%.
(2) by Thermostable α-Amylase and anhydrous calcium chloride compounding use, first simultaneously for both consumption and addition
Order, addition temperature all make collaborative design afterwards, particularly with Thermostable α-Amylase using different amounts under the conditions of dividing two
Feed postition, ultrasonic Treatment in additional coordinated, beneficial to the liquefaction of the high-concentration starch milk being adopted, reduce and liquefied
Journey viscosity, improves liquefaction process de value；Liquefaction value increases 12.3%-14.8%, and peak viscosity reduces 38.1-40.1%, de
Value increases 63.1-67.4%.
(3) yield rate of saccharifying gained starch sugar is 109.3-112.5%, and wherein glucose yield reaches 96.81-
97.69%.
(4) 95.8-97.1% is reached using light transmittance after Powdered Activated Carbon ak-220 decolouring.
(5) farthest make use of heat energy in whole technique: the indirect steam waste heat after stage flash distillation of being refined sugar by starch
To evaporation feed liquor preheating；Part utilizes liquefier high temperature material heat energy to heat up to saccharifying discharging；Using liquefaction cooling hot water successively
Heat to starch milk, fermentation liquid；Using even disappearing, cooling hot water is heated to starch milk, fermentation liquid successively.Sodium gluconate life first
In the starch sugaring stage produced, preheated to fermentation liquid evaporation feed liquor with the indirect steam waste heat after flash distillation, true using vaporizer
Empty liquefaction indirect steam is inhaled toward evaporation preheater, and parameter is set to: indirect steam >=100 DEG C, evaporation feed liquor temperature 60-75
DEG C, input concentration 30-35%.Draw according to material heat balance, 8-15 DEG C of evaporation feed liquor intensification, be equivalent to minimizing and be used for thing
The steam usage amount of 8-15 DEG C of intensification of material；Secondly the starch sugaring stage that sodium gluconate produces, with 95-99 DEG C of liquefaction liquid material with
60 ± 3 DEG C of saccharifying feed liquids carry out plate type heat exchanger heat exchange, mass flow equity heat exchange, and heat exchanger area presses sugar liquid flow every 1
m3/ h converts plate type heat exchanger 2.5, and after heat exchange, liquefaction liquid temp is down to 80-85 DEG C by 95-99 DEG C, and sugar liquid temperature is by 60 ± 2
DEG C rise to 75-80 DEG C, saccharifying feed liquid finally raises 15-20 DEG C, having saved fermentation and even disappear and having used quantity of steam；Sodium gluconate life again
In the starch sugaring stage produced, first enter andante and change to heat to the starch milk before liquefaction with liquefier 50-60 DEG C of hot water of lowering the temperature, then liquid
Change cooling hot water to be concatenated entering andante heat exchange with 37-39 DEG C of fermentation liquid, comprehensive two heat exchange, starch milk and fermentation liquid are final
Accumulative intensification reaches >=12 DEG C；Last sodium gluconate produce in fermentation stage even disappear 50-60 DEG C of hot water of cooling first to liquefaction before
Starch milk enter andante and change to heat, then connecting the cooling hot water that disappears and be concatenated with fermentation liquid 37-39 DEG C and entering andante heat exchange, comprehensive two
Platen heat exchange, starch milk and fermentation liquid finally add up intensification and reach >=10 DEG C.
In sum, the present invention energy saving and environment friendly Production by Enzymes sodium gluconate new technology compared to traditional catalysis method and
Fermentation method has safe, and product is easy to extract and refined significant advantage, increases substantially the quality of sodium gluconate, with
When fully utilize liquefaction flash indirect steam during sodium gluconate produces, High-temperature Liquefaction liquid and liquefaction cooling hot water and fermentation
Connect the heat of the generation that disappears, the steam consumption of product can be reduced so that product cost is greatly lowered.Using the life of this new technology
Malaga sodium saccharate, ton finished product steam consumption reduces 0.28 ton, according to annual capacity 1 year steam saving of 100,000 tons of line computations
28000 tons, reduce entreprise cost, decrease carbon emission, thus being conducive to the protection of environment, obvious using this invention effect.
Brief description
Fig. 1 is heat energy in energy saving and environment friendly Production by Enzymes sodium gluconate new technology described in the specific embodiment of the invention
Using process route chart.
Specific embodiment
Below by embodiment, the present invention will be described in detail.
Described energy saving and environment friendly Production by Enzymes sodium gluconate new technology, comprises the following steps:
(1) starch milk liquefaction, saccharifying: a. liquefaction: adopt 50-60 DEG C of high-temperature-hot-water that raw material is formed sediment first in plate type heat exchanger
Powder breast carries out heat exchange and is heated up to 40-45 DEG C, then raw starch breast is sized mixing to mass fraction and is delivered to liquefaction for after 40-44%
In ejector, adjusting ph value is 4.8-5.0, is successively sequentially added into 11-13u/g Thermostable α-Amylase and mass fraction is
0.05-0.08% anhydrous calcium chloride carries out the 15-24 minute that liquefies at 80-95 DEG C, simultaneously at ultrasound wave under the conditions of power 240w
Reason 6-10 minute；Add 12-15u/g Thermostable α-Amylase to carry out liquefaction 12-16 at finally adjusting the temperature to 100-110 DEG C to divide
Clock obtains liquefier；
B. flash distillation, cooling: 100-110 DEG C of liquefier entrance flash tank of gained carries out flash distillation and respectively obtain secondary flash vapour and 95-
99 DEG C of flash distillation liquefiers；95-99 DEG C of flash distillation liquefier first passes around plate type heat exchanger heat exchange and is cooled to 80-85 DEG C, Ran Houzai
It is cooled to 58-62 DEG C via plate type heat exchanger heat exchange；
C. saccharifying: 58-62 DEG C of flash distillation liquefier of gained is delivered in saccharifying tank, adjusting ph value at 60 DEG C of constant temperature is 4.0-
4.2, successively add 0.331ausp pullulanase, 218.1u/g beta amylase and 51.32u/g amyloglucosidase successively, enter
Row enzymatic saccharification 3.5-5.5 hour, obtains 58-62 DEG C of saccharified liquid, this saccharified liquid is warming up to 75- through plate type heat exchanger heat exchange
80 DEG C, wherein contained by gained saccharified liquid, glucose quality fraction is 35%-37%；
(2) even disappear: 75-80 DEG C of saccharified liquid of gained being delivered to fermentation even disappear and carry out even disappearing in ejector, the sugar after even disappearing
Change liquid carries out heat exchange through plate type heat exchanger and is cooled to 37-39 DEG C；
(3) ferment: a. enzyme preparation a: penicillium sp and aspergillus niger are placed in container than 1.5:1 according to weight, by every gram of mycelia
The ratio of 3.0ml, adds the phosphate buffer of ph5.1, freezes 15 minutes, room temperature is melted, repeatedly twice at being positioned over -25 DEG C；
Then the purification of ethanol being 70% using volume fraction after ultrasonication 5-8 minute under ultrasonic power 400w, using quality
Fraction is that 14% Polyethylene Glycol secondarily purified obtains purification glucoseoxidase liquid；Purification glucoseoxidase liquid adds
Catalase obtains double enzyme liquids, and wherein catalatic is 0.05g/ml to enzyme amount；Add after taking hydroxyapatite sterilizing
To double enzyme liquids, wherein solid-to-liquid ratio is 1:10；Stand 2-3.5 hour at 3 DEG C, be centrifuged under the conditions of 3500r after natural subsidence
So that impregnated carrier is separated with clear liquid within 10 minutes, obtain impregnated carrier；
B. saccharified liquid fermentation: saccharified liquid is added in fermentation tank, is incubated 38-39 DEG C, is passed through oxygen 0.012m3/ min, rotating speed
100r/min, treats dissolved oxygen stable post-equalization dissolved oxygen 100%；Stop ventilation, pressure release, add impregnated carrier, wherein solid-to-liquid ratio is 1:
20；Recover ventilation, keep tank pressure 0.15 mpa, add defoamer 0.3 ml, keep ph value to be 4.5-5.0, control temperature to exist
38-39 DEG C carries out fermentation and obtains fermentation liquid；38-39 DEG C of fermentation liquid is warming up to 45-50 DEG C through plate type heat exchanger heat exchange；
(4) decolour: using Powdered Activated Carbon ak-220 in temperature 45-50 DEG C, ph value is sending out after heat exchanging under the conditions of 4.5-5.2
Zymotic fluid decolouring 20-35 minute；
(5) concentrate: the fermentation liquid after decolouring enters vaporizer and is evaporated concentrating, and wherein fermentation liquid first passes around evaporation preheater
It is preheated to 60-75 DEG C, evaporation preheater adopts secondary flash vapour waste heat；
(6) filtration, washing, drying, finished product.
In described step (1), the high-temperature-hot-water of plate type heat exchanger is by plate type heat exchanger liquefier heat exchange temperature raising medium water
With plate type heat exchanger saccharified liquid heat exchange temperature raising medium water composition.
In described step (1), the heat exchange low temperature water outlet of plate type heat exchanger is entered in plate type heat exchanger and is carried out with fermentation liquid
Heat exchange.
The solid-to-liquid ratio of the fermentation liquid after Powdered Activated Carbon ak-220 and heat exchange in the decolouring of described step (4) is 1:15.
In described step (1), the temperature of secondary flash vapour is more than 100 DEG C.
In described step (1), ultrasonic Treatment is specially and for ultrasonic variable amplitude bar to insert below starch milk liquid level 2.5-3
Cm, magnetic agitation rotating speed 50%.
Wherein heat energy utilization situation in detail is as follows:
(1) starch milk mixing up is preheated through plate type heat exchanger, and temperature rises 12-18 DEG C, rises to through liquefaction ejector
125 DEG C, subsequently into flash tank liquefier instantaneous temperature reduction to 95-99 DEG C, the indirect steam devaporation device lightening is to gluconic acid
Sodium solution preheats, and sodium gluconate solution raises 15 DEG C.
(2) after flash tank, 95-99 DEG C of liquefier enters plate type heat exchanger and 60 ± 2 DEG C of saccharified liquids carry out heat exchange,
Mass flow equity heat exchange, after heat exchange, liquefaction liquid temp is down to 80-85 DEG C by 95-99 DEG C, and sugar liquid temperature is risen to by 60 ± 3 DEG C
75-80 DEG C, saccharifying feed liquid finally raises 15-20 DEG C.
(3) 80-85 DEG C of liquefier is cooled to 60 ± 3 DEG C through plate type heat exchanger by cold water, enters saccharifying tank, cold water is through changing
After heat, temperature rises to 50-60 DEG C, and hot water fully enters plate type heat exchanger after concentrating, and hot water out enters plate from plate type heat exchanger
Formula heat exchanger, completes to starch milk and fermentation liquid heating.
(4) sugar liquid, after the preliminary heating of plate type heat exchanger, enters fermentation and connects the ejector that disappears, even disappear and rise to 110 DEG C, sterilize
Terminate to enter plate type heat exchanger cooling, enter fermentation tank after cooling, after cold water enters plate type heat exchanger, temperature rises to 50-60
DEG C, hot water respectively enters plate type heat exchanger, plate type heat exchanger in order.Final starch milk and fermentation liquid add up intensification reach >=
22℃.
Produce sodium gluconate with this device with production method, ton finished product steam consumption reduces 0.28 ton, according to annual output
28000 tons of 1 year steam saving of 100,000 tons of line computations of ability, obvious using this invention effect.
Claims (6)
1. a kind of energy saving and environment friendly Production by Enzymes sodium gluconate new technology, comprises the following steps:
(1) starch milk liquefaction, saccharifying: a. liquefaction: adopt 50-60 DEG C of high-temperature-hot-water that raw material is formed sediment first in plate type heat exchanger
Powder breast carries out heat exchange and is heated up to 40-45 DEG C, then raw starch breast is sized mixing to mass fraction and is delivered to liquefaction for after 40-44%
In ejector, adjusting ph value is 4.8-5.0, is successively sequentially added into 11-13u/g Thermostable α-Amylase and mass fraction is
0.05-0.08% anhydrous calcium chloride carries out the 15-24 minute that liquefies at 80-95 DEG C, simultaneously at ultrasound wave under the conditions of power 240w
Reason 6-10 minute；Add 12-15u/g Thermostable α-Amylase to carry out liquefaction 12-16 at finally adjusting the temperature to 100-110 DEG C to divide
Clock obtains liquefier；
B. flash distillation, cooling: 100-110 DEG C of liquefier entrance flash tank of gained carries out flash distillation and respectively obtain secondary flash vapour and 95-
99 DEG C of flash distillation liquefiers；95-99 DEG C of flash distillation liquefier first passes around plate type heat exchanger heat exchange and is cooled to 80-85 DEG C, Ran Houzai
It is cooled to 58-62 DEG C via plate type heat exchanger heat exchange；
C. saccharifying: 58-62 DEG C of flash distillation liquefier of gained is delivered in saccharifying tank, adjusting ph value at 60 DEG C of constant temperature is 4.0-
4.2, successively add 0.331ausp pullulanase, 218.1u/g beta amylase and 51.32u/g amyloglucosidase successively, enter
Row enzymatic saccharification 3.5-5.5 hour, obtains 58-62 DEG C of saccharified liquid, this saccharified liquid is warming up to 75- through plate type heat exchanger heat exchange
80 DEG C, wherein contained by gained saccharified liquid, glucose quality fraction is 35%-37%；
(2) even disappear: 75-80 DEG C of saccharified liquid of gained being delivered to fermentation even disappear and carry out even disappearing in ejector, the sugar after even disappearing
Change liquid carries out heat exchange through plate type heat exchanger and is cooled to 37-39 DEG C；
(3) ferment: a. enzyme preparation a: penicillium sp and aspergillus niger are placed in container than 1.5:1 according to weight, by every gram of mycelia
The ratio of 3.0ml, adds the phosphate buffer of ph value 5.1, frost 15 minutes at being positioned over -25 DEG C, and room temperature is melted, and repeatedly two
Secondary；Then the purification of ethanol being 70% using volume fraction after ultrasonication 5-8 minute under ultrasonic power 400w, adopts
Mass fraction is that 14% Polyethylene Glycol secondarily purified obtains purification glucoseoxidase liquid；In purification glucoseoxidase liquid
Catalase is added to obtain double enzyme liquids, wherein catalatic is 0.05g/ml to enzyme amount；After taking hydroxyapatite sterilizing
Add to double enzyme liquids, wherein solid-to-liquid ratio is 1:10；Stand 2-3.5 hour at 3 DEG C, after natural subsidence under the conditions of 3500r
Centrifugation makes impregnated carrier separate with clear liquid in 10 minutes, obtains impregnated carrier；
B. saccharified liquid fermentation: saccharified liquid is added in fermentation tank, is incubated 38-39 DEG C, is passed through oxygen 0.012m3/ min, rotating speed
100r/min, treats dissolved oxygen stable post-equalization dissolved oxygen 100%；Stop ventilation, pressure release, add impregnated carrier, wherein solid-to-liquid ratio is 1:
20；Recover ventilation, keep tank pressure 0.15 mpa, add defoamer 0.3 ml, keep ph value to be 4.5-5.0, control temperature to exist
38-39 DEG C carries out fermentation and obtains fermentation liquid；38-39 DEG C of fermentation liquid is warming up to 45-50 DEG C through plate type heat exchanger heat exchange；
(4) decolour: using Powdered Activated Carbon ak-220 in temperature 45-50 DEG C, ph value is sending out after heat exchanging under the conditions of 4.5-5.2
Zymotic fluid decolouring 20-35 minute；
(5) concentrate: the fermentation liquid after decolouring enters vaporizer and is evaporated concentrating, and wherein fermentation liquid first passes around evaporation preheater
It is preheated to 60-75 DEG C, evaporation preheater adopts secondary flash vapour waste heat；
(6) filtration, washing, drying, finished product.
2. according to claim 1 energy saving and environment friendly Production by Enzymes sodium gluconate new technology it is characterised in that described step
(1) in, the high-temperature-hot-water of plate type heat exchanger is by plate type heat exchanger liquefier heat exchange temperature raising medium water and plate type heat exchanger sugar
Change liquid heat exchange temperature raising medium water composition.
3. according to claim 2 energy saving and environment friendly Production by Enzymes sodium gluconate new technology it is characterised in that described step
(1) in, the heat exchange low temperature water outlet of plate type heat exchanger enters in plate type heat exchanger and carries out heat exchange with fermentation liquid.
4. according to claim 1 energy saving and environment friendly Production by Enzymes sodium gluconate new technology it is characterised in that described step
(4) solid-to-liquid ratio of the fermentation liquid after Powdered Activated Carbon ak-220 and heat exchange in decolouring is 1:15.
5. according to claim 1 energy saving and environment friendly Production by Enzymes sodium gluconate new technology it is characterised in that described step
(1) in, the temperature of secondary flash vapour is more than 100 DEG C.
6. according to claim 1 energy saving and environment friendly Production by Enzymes sodium gluconate new technology it is characterised in that described step
(1) in, ultrasonic Treatment is specially and for ultrasonic variable amplitude bar to insert below starch milk liquid level 2.5-3 cm, magnetic agitation rotating speed
50%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109762736A (en) * | 2019-03-21 | 2019-05-17 | 石家庄吉瑞节能技术有限公司 | Liquefying starchiness raw material-saccharification-bactericidal unit |
| CN110441250A (en) * | 2019-06-13 | 2019-11-12 | 吉林大学 | A kind of preparation method that double enzymes are total to fixed copper nanometer floral material and the application in glucose detection |
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| CN103667375A (en) * | 2013-11-26 | 2014-03-26 | 郑州市中食农产品加工研究院 | Method for preparing sodium gluconate by adopting aspergillus niger fermentation method |
| CN104830918A (en) * | 2015-04-07 | 2015-08-12 | 山东西王糖业有限公司 | Novel production method of sodium gluconate |
| CN105002226A (en) * | 2015-06-25 | 2015-10-28 | 山东省食品发酵工业研究设计院 | Method for sterilization of medium for production of sodium gluconate by Aspergillus niger fermentation |
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| CN103667375A (en) * | 2013-11-26 | 2014-03-26 | 郑州市中食农产品加工研究院 | Method for preparing sodium gluconate by adopting aspergillus niger fermentation method |
| CN104830918A (en) * | 2015-04-07 | 2015-08-12 | 山东西王糖业有限公司 | Novel production method of sodium gluconate |
| CN105002226A (en) * | 2015-06-25 | 2015-10-28 | 山东省食品发酵工业研究设计院 | Method for sterilization of medium for production of sodium gluconate by Aspergillus niger fermentation |
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Cited By (3)
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| CN109762736A (en) * | 2019-03-21 | 2019-05-17 | 石家庄吉瑞节能技术有限公司 | Liquefying starchiness raw material-saccharification-bactericidal unit |
| CN110441250A (en) * | 2019-06-13 | 2019-11-12 | 吉林大学 | A kind of preparation method that double enzymes are total to fixed copper nanometer floral material and the application in glucose detection |
| CN110441250B (en) * | 2019-06-13 | 2021-11-09 | 吉林大学 | Preparation method of double-enzyme co-immobilized copper nanoflower material and application of double-enzyme co-immobilized copper nanoflower material in glucose detection |
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