CN112625980A - Process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum - Google Patents
Process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum Download PDFInfo
- Publication number
- CN112625980A CN112625980A CN202110057679.8A CN202110057679A CN112625980A CN 112625980 A CN112625980 A CN 112625980A CN 202110057679 A CN202110057679 A CN 202110057679A CN 112625980 A CN112625980 A CN 112625980A
- Authority
- CN
- China
- Prior art keywords
- culture
- fermentation
- stage
- percent
- seed culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 67
- 230000004151 fermentation Effects 0.000 title claims abstract description 67
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 title claims abstract description 65
- 241000193744 Bacillus amyloliquefaciens Species 0.000 title claims abstract description 25
- 241000193171 Clostridium butyricum Species 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000003501 co-culture Methods 0.000 title claims abstract description 12
- 238000011218 seed culture Methods 0.000 claims abstract description 49
- 239000001963 growth medium Substances 0.000 claims abstract description 26
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 12
- 239000008103 glucose Substances 0.000 claims abstract description 12
- 229920002472 Starch Polymers 0.000 claims abstract description 11
- 235000019698 starch Nutrition 0.000 claims abstract description 11
- 239000008107 starch Substances 0.000 claims abstract description 11
- 235000000346 sugar Nutrition 0.000 claims abstract description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 6
- 229920002261 Corn starch Polymers 0.000 claims description 4
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 4
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 4
- 239000002518 antifoaming agent Substances 0.000 claims description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 4
- 239000008120 corn starch Substances 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 4
- 239000011565 manganese chloride Substances 0.000 claims description 4
- 235000002867 manganese chloride Nutrition 0.000 claims description 4
- 229940099607 manganese chloride Drugs 0.000 claims description 4
- 229940099596 manganese sulfate Drugs 0.000 claims description 4
- 239000011702 manganese sulphate Substances 0.000 claims description 4
- 235000007079 manganese sulphate Nutrition 0.000 claims description 4
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims 5
- 238000011081 inoculation Methods 0.000 abstract description 12
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 239000002994 raw material Substances 0.000 abstract description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- 238000009776 industrial production Methods 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000282849 Ruminantia Species 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000020912 omnivore Nutrition 0.000 description 1
- 244000054334 omnivore Species 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 210000004767 rumen Anatomy 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/52—Propionic acid; Butyric acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum, which comprises the following steps: the fermentation process is three-stage, and comprises first-stage seed culture, second-stage seed culture and fermentation culture, shake flask seed culture of bacillus amyloliquefaciens and clostridium butyricum, inoculation in a first-stage seed culture medium for first-stage seed culture, then inoculation in a second-stage seed culture medium for second-stage seed culture, and finally inoculation in a fermentation tank culture medium for fermentation culture, wherein the fermentation culture is divided into two stages, the first-stage culture temperature is 30-35 ℃, when the fermentation pH is reduced to 4.5-5.0, reducing sugar is controlled for 10-20 hours, the temperature is raised to 35-37 ℃, the second-stage culture is carried out, and the pH is controlled to 6.0. The invention utilizes starch to produce micromolecular sugar to replace glucose in a fermentation culture medium, thereby reducing the cost of the glucose in the raw material, increasing the production efficiency of butyric acid, and mainly solving the problems of reducing the production cost and improving the fermentation efficiency.
Description
Technical Field
The invention belongs to the technical field of butyric acid fermentation, and particularly relates to a process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum.
Background
Butyric acid is a product of microbial fermentation of carbohydrates in the rumen of ruminants and the colon of omnivores, has a strong bactericidal effect, and is considered as a potential substitute of antibiotics. The combination of butyric acid and antibiotics can improve the curative effect, and a large number of experiments show that the butyric acid has no toxic or side effect and is widely applied to medicines, functional health-care foods and additives of animal feeds. At present, butyric acid fermentation culture still mostly stays at a laboratory stage, the product concentration is basically about 10-30g/L, and the conditions of low fermentation efficiency and high cost exist in industrial production.
At present, the fermentation production of butyric acid is anaerobic culture, and thalli cannot grow or even die in the presence of oxygen in a fermentation environment; in addition, the pH of the cells is critical for optimal growth, and if the pH deviates from the optimal pH, the target product may not be obtained. The fermentation process is three-stage fermentation, and the first-stage seed culture process comprises the following steps: the temperature is 37 ℃, the anaerobic reaction is carried out, the rotating speed is 200rpm/min, the ph is natural, and the culture time is 20-24 h; secondary seed culture: the temperature is 37 ℃, the rotating speed is 200rpm/min, the culture period is 10-24h, the ORP is controlled to be-300 to-500 mv, and the pH is controlled to be 5.90; fermentation culture: the temperature is 37 ℃, the rotation speed is 150-; and (4) detecting the content of butyric acid after fermentation is finished, and adding a proper amount of sodium salt or calcium salt according to the concentration of the butyric acid to obtain the butyrate. The existing butyric acid fermentation technology has more byproducts, lower production efficiency and higher production cost, and is not suitable for modern industrial production.
Disclosure of Invention
The invention aims to provide a process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum, which has the advantages of simple fermentation condition, low cost and stable product quality and is suitable for industrial production.
In order to achieve the purpose, the invention adopts the technical scheme that:
the process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum comprises the following steps: the fermentation process is three-stage, and comprises first-stage seed culture, second-stage seed culture and fermentation culture, wherein the shake flask seed culture of the bacillus amyloliquefaciens and the clostridium butyricum is carried out for 17 hours, the first-stage seed culture is carried out by inoculating the bacillus amyloliquefaciens and the clostridium butyricum with the inoculation amount of 0.25 percent, then the second-stage seed culture is carried out by inoculating the bacillus amyloliquefaciens and the clostridium butyricum with the inoculation amount of 10 percent, the fermentation culture is carried out in a fermentation tank culture medium, the inoculation amount is 20 percent, the fermentation culture is divided into two stages, the culture temperature of the first stage is 30-35 ℃, when the fermentation pH is reduced to 4.5-5.0, the 10-20 hours are controlled, the reducing sugar is heated to 35-37 ℃, the second.
Further, the composition of the primary seed culture medium comprises: 0.5 to 0.6 percent of glucose, 1 to 2 percent of yeast extract powder, 0.5 to 1.0 percent of peptone and 0.05 to 0.2 percent of sodium chloride.
Further, the composition of the secondary seed culture medium comprises: 1 to 2 percent of yeast extract powder, 0.5 to 1 percent of peptone, 0.1 to 0.2 percent of soluble starch, 0.4 to 0.6 percent of glucose, 0.01 to 0.2 percent of manganese chloride, 0.05 to 0.06 percent of sodium chloride and 0.3 to 0.4 percent of sodium acetate.
Further, the composition of the fermenter medium comprises: 20% of starch, 1% of corn starch, 0.1-0.2% of ammonium sulfate, 0.4-0.6% of peptone, 0.1-0.2% of yeast powder, 0.1-0.2% of monopotassium phosphate, 0.2-0.4% of calcium carbonate, 0.07-0.8% of magnesium sulfate, 0.01-0.03% of manganese sulfate and 0.1-0.3% of defoaming agent. Further, the conditions of the primary seed culture are as follows: the culture temperature is 37 ℃, the rotation speed is 200rpm, and the culture time is 24-48 hours.
Further, the conditions of the secondary seed culture are as follows: the culture temperature was 37 ℃, the rotation speed was 200rpm, and the culture time was 24 hours.
Further, the second stage culture conditions of the fermentation culture are as follows: the pressure is 0.01Mpa, the rotating speed is 200rpm, and the culture time is 48 hours.
The invention has the advantages that:
the process for producing butyric acid by co-culture fermentation of the bacillus amyloliquefaciens and the clostridium butyricum provided by the invention comprises the steps of co-culture fermentation of the bacillus amyloliquefaciens and the clostridium butyricum, controlling the temperature to be 30 ℃ at the early stage of fermentation culture, promoting the bacillus amyloliquefaciens to carry out enzymolysis on starch into micromolecule sugar, detecting indexes of total sugar and reducing sugar, and controlling the optimal culture condition of a butyric acid strain at the middle and later stages to carry out butyric acid fermentation.
The invention utilizes starch to produce micromolecular sugar to replace glucose in a fermentation culture medium, thereby reducing the cost of the glucose in the raw material, increasing the production efficiency of butyric acid, and mainly solving the problems of reducing the production cost and improving the fermentation efficiency. The normal process comprises the steps of putting the tank into a fermentation tank with the butyric acid content of 45-50g/L, the acetic acid content of 5g/L and the lactic acid content of 2-3g/L, detecting the butyric acid content of 53-55g/L, the acetic acid content of 7g/L and the lactic acid content of 1-2g/L by HPLC (high performance liquid chromatography), and the experimental result shows that the content of the butyric acid in the tank after the improvement of the process is improved by about 10 percent compared with that in the initial process. The invention has simple fermentation condition, low cost and stable product quality, and is suitable for industrial production.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the specific examples described herein are only for explaining the present invention and are not intended to limit the present invention.
Example 1
The invention discloses a process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum, which comprises the following steps: the fermentation process is three-stage, including first-stage seed culture, second-stage seed culture and fermentation culture. The shake flask seeds of the bacillus amyloliquefaciens and the clostridium butyricum are cultured for 17 hours and are inoculated in a first-level seed culture medium for first-level seed culture, the inoculation amount is 0.25 percent, and the first-level seed culture medium comprises the following components: 0.5% of glucose, 1% of yeast extract powder, 0.5% of peptone and 0.05% of sodium chloride, wherein the conditions of primary seed culture are as follows: the culture temperature was 37 ℃, the rotation speed was 200rpm, and the culture time was 24 hours.
Then inoculating the strain in a secondary seed culture medium for secondary seed culture, wherein the inoculation amount is 10 percent, and the secondary seed culture medium comprises the following components: 1% of yeast extract powder, 0.5% of peptone, 0.1% of soluble starch, 0.4% of glucose, 0.01% of manganese chloride, 0.05% of sodium chloride and 0.3% of sodium acetate. The conditions for secondary seed culture are as follows: the culture temperature was 37 ℃, the rotation speed was 200rpm, and the culture time was 24 hours.
Finally inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the inoculation amount is 20%, and the fermentation tank culture medium comprises the following components: 20% of starch, 1% of corn starch, 0.1% of ammonium sulfate, 0.4% of peptone, 0.1% of yeast powder, 0.1% of monopotassium phosphate, 0.2% of calcium carbonate, 0.07% of magnesium sulfate, 0.01% of manganese sulfate and 0.1% of defoaming agent. The fermentation culture is divided into two stages, the first stage culture temperature is 30 ℃, when the fermentation pH is reduced to 4.5, reducing sugar is controlled for 10 hours, the temperature is raised to 35 ℃, the second stage culture is carried out, the pH is controlled to 6.0, the pressure is 0.01Mpa, the rotating speed is 200rpm, and the culture time is 48 hours.
Example 2
The invention discloses a process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum, which comprises the following steps: the fermentation process is three-stage, including first-stage seed culture, second-stage seed culture and fermentation culture. The shake flask seeds of the bacillus amyloliquefaciens and the clostridium butyricum are cultured for 17 hours and are inoculated in a first-level seed culture medium for first-level seed culture, the inoculation amount is 0.25 percent, and the first-level seed culture medium comprises the following components: 0.6 percent of glucose, 2 percent of yeast extract powder, 1.0 percent of peptone and 0.2 percent of sodium chloride. The conditions for primary seed culture are as follows: the culture temperature was 37 ℃, the rotation speed was 200rpm, and the culture time was 48 hours.
Then inoculating the strain in a secondary seed culture medium for secondary seed culture, wherein the inoculation amount is 10 percent, and the secondary seed culture medium comprises the following components: 2% of yeast extract powder, 1% of peptone, 0.2% of soluble starch, 0.6% of glucose, 0.2% of manganese chloride, 0.06% of sodium chloride and 0.4% of sodium acetate. The conditions for secondary seed culture are as follows: the culture temperature was 37 ℃, the rotation speed was 200rpm, and the culture time was 24 hours.
Finally inoculating the strain into a fermentation tank culture medium for fermentation culture, wherein the inoculation amount is 20%, and the fermentation tank culture medium comprises the following components: 20% of starch, 1% of corn starch, 0.2% of ammonium sulfate, 0.6% of peptone, 0.2% of yeast powder, 0.2% of monopotassium phosphate, 0.4% of calcium carbonate, 0.8% of magnesium sulfate, 0.03% of manganese sulfate and 0.3% of defoaming agent. The fermentation culture is divided into two stages, the first stage culture temperature is 35 ℃, when the fermentation pH is reduced to 5.0, reducing sugar is controlled for 20 hours, the temperature is raised to 37 ℃, the second stage culture is carried out, the pH is controlled to 6.0, the pressure is 0.01Mpa, the rotating speed is 200rpm, and the culture time is 48 hours.
Claims (7)
1. The process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum comprises the following steps: the fermentation process is three-stage, and comprises first-stage seed culture, second-stage seed culture and fermentation culture, and is characterized in that the shaking flask seed culture of the bacillus amyloliquefaciens and the clostridium butyricum is inoculated in a first-stage seed culture medium for first-stage seed culture, then is inoculated in a second-stage seed culture medium for second-stage seed culture, and finally is inoculated in a fermentation tank culture medium for fermentation culture, the fermentation culture is divided into two stages, the first stage culture temperature is 30-35 ℃, when the fermentation pH is reduced to 4.5-5.0, the reducing sugar is controlled for 10-20 hours, the temperature is raised to 35-37 ℃, the second stage culture is carried out, and the pH is controlled to 6.0.
2. The process for producing butyric acid by co-culturing and fermenting bacillus amyloliquefaciens and clostridium butyricum according to claim 1, wherein the composition of the primary seed culture medium comprises: 0.5 to 0.6 percent of glucose, 1 to 2 percent of yeast extract powder, 0.5 to 1.0 percent of peptone and 0.05 to 0.2 percent of sodium chloride.
3. The process for producing butyric acid by co-culturing and fermenting bacillus amyloliquefaciens and clostridium butyricum according to claim 1, wherein the composition of the secondary seed culture medium comprises: 1 to 2 percent of yeast extract powder, 0.5 to 1 percent of peptone, 0.1 to 0.2 percent of soluble starch, 0.4 to 0.6 percent of glucose, 0.01 to 0.2 percent of manganese chloride, 0.05 to 0.06 percent of sodium chloride and 0.3 to 0.4 percent of sodium acetate.
4. The process for producing butyric acid by co-culturing and fermenting bacillus amyloliquefaciens and clostridium butyricum according to claim 1, wherein the composition of the fermentation tank culture medium comprises: 20% of starch, 1% of corn starch, 0.1-0.2% of ammonium sulfate, 0.4-0.6% of peptone, 0.1-0.2% of yeast powder, 0.1-0.2% of monopotassium phosphate, 0.2-0.4% of calcium carbonate, 0.07-0.8% of magnesium sulfate, 0.01-0.03% of manganese sulfate and 0.1-0.3% of defoaming agent.
5. The process for producing butyric acid by co-culturing and fermenting bacillus amyloliquefaciens and clostridium butyricum according to claim 1, wherein the conditions of the primary seed culture are as follows: the culture temperature is 37 ℃, the rotation speed is 200rpm, and the culture time is 24-48 hours.
6. The process for producing butyric acid by co-culturing and fermenting bacillus amyloliquefaciens and clostridium butyricum according to claim 1, wherein the secondary seed culture conditions are as follows: the culture temperature was 37 ℃, the rotation speed was 200rpm, and the culture time was 24 hours.
7. The process for producing butyric acid by co-culture and fermentation of bacillus amyloliquefaciens and clostridium butyricum according to claim 1, wherein the second-stage culture conditions of the fermentation culture are as follows: the pressure is 0.01Mpa, the rotating speed is 200rpm, and the culture time is 48 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110057679.8A CN112625980A (en) | 2021-01-15 | 2021-01-15 | Process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110057679.8A CN112625980A (en) | 2021-01-15 | 2021-01-15 | Process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112625980A true CN112625980A (en) | 2021-04-09 |
Family
ID=75294454
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110057679.8A Pending CN112625980A (en) | 2021-01-15 | 2021-01-15 | Process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112625980A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410539A (en) * | 2022-01-29 | 2022-04-29 | 山东泰山生力源集团股份有限公司 | Preparation method of clostridium butyricum solid fermentation material and fermentation material |
| CN114698729A (en) * | 2022-03-30 | 2022-07-05 | 播恩集团股份有限公司 | Microecological preparation containing 5-methyltetrahydrofolic acid and preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090035812A1 (en) * | 2007-08-03 | 2009-02-05 | National Chung Hsing University | Microbial hydrogen-producing process and system thereof |
| CN103627656A (en) * | 2013-11-06 | 2014-03-12 | 天津科技大学 | Solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans |
| US20160068919A1 (en) * | 2014-09-05 | 2016-03-10 | Green Cellulosity Corporation | Microorganism co-culture system and uses of the same |
| CN108504697A (en) * | 2018-04-20 | 2018-09-07 | 安徽天邦生物技术有限公司 | A kind of clostridium butyricum fermentation process significantly improving butyric acid yield |
| CN109825447A (en) * | 2017-11-23 | 2019-05-31 | 朝阳华星生物工程有限公司 | A kind of fermentation manufacturing technique of high yield clostridium butyricum |
| CN111500508A (en) * | 2020-05-31 | 2020-08-07 | 郑州大学 | Liquid mixed fermentation method of clostridium butyricum and bacillus coagulans |
-
2021
- 2021-01-15 CN CN202110057679.8A patent/CN112625980A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090035812A1 (en) * | 2007-08-03 | 2009-02-05 | National Chung Hsing University | Microbial hydrogen-producing process and system thereof |
| CN103627656A (en) * | 2013-11-06 | 2014-03-12 | 天津科技大学 | Solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans |
| US20160068919A1 (en) * | 2014-09-05 | 2016-03-10 | Green Cellulosity Corporation | Microorganism co-culture system and uses of the same |
| CN109825447A (en) * | 2017-11-23 | 2019-05-31 | 朝阳华星生物工程有限公司 | A kind of fermentation manufacturing technique of high yield clostridium butyricum |
| CN108504697A (en) * | 2018-04-20 | 2018-09-07 | 安徽天邦生物技术有限公司 | A kind of clostridium butyricum fermentation process significantly improving butyric acid yield |
| CN111500508A (en) * | 2020-05-31 | 2020-08-07 | 郑州大学 | Liquid mixed fermentation method of clostridium butyricum and bacillus coagulans |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410539A (en) * | 2022-01-29 | 2022-04-29 | 山东泰山生力源集团股份有限公司 | Preparation method of clostridium butyricum solid fermentation material and fermentation material |
| CN114410539B (en) * | 2022-01-29 | 2024-04-12 | 山东泰山生力源集团股份有限公司 | Preparation method of clostridium butyricum solid fermentation material and fermentation material |
| CN114698729A (en) * | 2022-03-30 | 2022-07-05 | 播恩集团股份有限公司 | Microecological preparation containing 5-methyltetrahydrofolic acid and preparation method and application thereof |
| CN114698729B (en) * | 2022-03-30 | 2023-01-10 | 播恩集团股份有限公司 | Microecological preparation containing 5-methyltetrahydrofolic acid and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110643547B (en) | Brevibacterium flavum for producing L-valine and method for producing L-valine by using same | |
| CN102174602A (en) | Method for producing L-lactic acid through biomass fermentation | |
| CN112625980A (en) | Process for producing butyric acid by co-culture fermentation of bacillus amyloliquefaciens and clostridium butyricum | |
| CN112695061A (en) | L-glutamic acid total nutrient fed-batch high-density fermentation method | |
| CN110982855A (en) | Biotransformation method for efficiently synthesizing gamma-aminobutyric acid | |
| CN103088089B (en) | Method for fermenting acarbose | |
| CN103898181A (en) | Method for producing nosiheptide by virtue of fermentation | |
| CN103789362B (en) | A kind of premashing and simultaneous saccharification and fermentation produce the method for lactic acid | |
| CN101705261B (en) | Preparation method of Gamma-aminobutyric acid | |
| CN104561140B (en) | A kind of method of preparation of citric acid by fermentation | |
| US8227220B2 (en) | Process for the preparation of ethanol from starch | |
| CN106893682B (en) | Method for expanding culture of saccharomycetes by using liquefied mash and application of saccharomycetes and method for fermenting ethanol | |
| CN109136313B (en) | Method for synthesizing 2' -deoxyadenosine by using klebsiella melitensis | |
| CN114196588B (en) | Thermophilic anaerobic clostridium succinate bacterial strain and method for producing succinic acid by using lignocellulose | |
| CN102051385B (en) | Method for producing lactic acid by fermentation of acorn powder | |
| CN105624213B (en) | A method of 2,3- butanediol is produced using microalgae for raw material | |
| CN112626137A (en) | Method for producing butyric acid by cascade fermentation of acetobacter and clostridium butyricum | |
| CN102586366B (en) | Method for producing erythrocin at high yield | |
| CN101643753B (en) | Preparation method for klinint | |
| CN114807098B (en) | Culture method for producing extracellular cellulose degrading enzyme system | |
| CN110923275A (en) | Fermentation and extraction process of glutamic acid | |
| CN104498542A (en) | Method for preparing L-lactic acid employing continuous method in fermentation manner | |
| JP6322187B2 (en) | Ethanol production method from cellulosic biomass | |
| CN112175999B (en) | Fermentation treatment method of corn soaking liquid, liquid phase product obtained by using fermentation treatment method and application of liquid phase product | |
| CN114181859B (en) | Geobacillus stearothermophilus and method for producing lactic acid by using lignocellulose |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |