CN101575621A - High concentration fermentation process of 2-keto-D-gluconic acid mixed salt - Google Patents
High concentration fermentation process of 2-keto-D-gluconic acid mixed salt Download PDFInfo
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- CN101575621A CN101575621A CNA2009101481154A CN200910148115A CN101575621A CN 101575621 A CN101575621 A CN 101575621A CN A2009101481154 A CNA2009101481154 A CN A2009101481154A CN 200910148115 A CN200910148115 A CN 200910148115A CN 101575621 A CN101575621 A CN 101575621A
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Abstract
The invention discloses a high concentration fermentation process of 2-keto-D-gluconic acid mixed salt, comprising the following steps: adding the culture medium which contains glucose, corn steep liquor and light calcium carbonate to a fermentation vessel; introducing the bacteria seeding liquid; adding sodium carbonate solution or sodium hydroxide solution in a fed-batch way when the light calcium carbonate in the fermentation vessel runs out; starting to add the glucose solution with the concentration of 300g/L to 500g/L in batches or in fed-batch way when the concentration of the glucose solution in the fermentation culture medium drops to 20g/L to 100g/L; controlling the gross sugar concentration of the fermentation fluid terminal point to 150g/L to 300g/L until the remaining sugar concentration is below 2g/L and the fermentation finishes. The invention solves the problem that the 2-keto-D-calcium gluconate salt is easy to be crystallized, improves the quality of D-iso ascorbic acid and the salt finished products thereof, and puts the discharge of solid waste calcium sulphate waste residue to an end.
Description
Technical field
The present invention relates to the preparation method of D-saccharosonic acid and its esters, specifically a kind of 2-keto-D-gluconic acid mixing salt high concentration fermentation process.
Background technology
The 2-keto-D-gluconic acid is a precursor of producing D-saccharosonic acid and its esters (as sodium D-isoascorbate), D-saccharosonic acid and its esters can be used as food antioxidant, anticorrosion, fresh-keeping, help toner.At present, known indirect fermentative Production D-saccharosonic acid of domestic and international industrial employing and its esters, its technological process is: at first utilize the glucose in the bacterium transforming fermentation culture base to be 2-ketone group-D-calisanin (adopting light calcium carbonate in the fermenting process as neutralizing agent), fermented liquid purifies fermented liquid through sulfuric acid acidation, centrifugal (filtration) etc., to remove calcium ion and other impurity in the fermented liquid, then fermented liquid cryoconcentration to the syrupy shape that purifies is used for the synthetic of SODIUM ISOVITAMIN C.Owing to the defective of fermented liquid purifying treatment method, cause in actual production, existing finished product D-saccharosonic acid and salt quality instability thereof, the many problems such as (mainly being the calcium sulfate waste residue) of solid waste in the above-mentioned technology.The domestic in recent years a kind of fermented liquid ultrafiltration purification process of development use that begins, its method is: 2-ketone group-D-calisanin fermented liquid is directly carried out ultrafiltration (organic membrane or ceramic membrane) handle, filtrate is obtained 2-keto-D-gluconic acid clear liquid through ion-exchange and activated carbon decolorizing.After in former D-saccharosonic acid and its esters production technique, introducing new fermented liquid purifying treatment method, final product quality has obtained large increase, solved the useless admittedly problems of calcium sulfate a large amount of in producing for a long time, but still there is following defective in this novel process: the 2-ketone group of the high density-easy crystallization of D-calisanin fermented liquid, reduced the ultrafiltration yield, and crystal causes damage to ultra-filtration membrane easily, influences ultra-filtration membrane work-ing life.Produce 2-keto-D-gluconic acid sodium fermented liquid with replacement light calcium carbonates such as yellow soda ash or sodium hydroxide as the neutralizing agent of 2-keto-D-gluconic acid, can solve ultrafiltration primary fermentation liquid crystalline problem, but pure 2-keto-D-gluconic acid sodium salt fermentation still exists technology instability, fermentation concentration to hang down the problems such as transformation efficiency is low that reach so far, this method is not adopted by suitability for industrialized production, and above problem has limited promoting the use of of novel process.
Summary of the invention
The purpose of this invention is to provide a kind of 2-keto-D-gluconic acid mixing salt high concentration fermentation process, on the basis that does not influence fermentation conversion rate, solved the easy crystalline problem of 2-keto-D-gluconic acid calcium salt in the fermented liquid.
Technical solution of the present invention is that this technology may further comprise the steps:
1) in fermenting container, adds a certain amount of substratum that contains glucose, corn steep liquor and light calcium carbonate, access can be the thin strain liquid of 2-keto-D-gluconic acid with conversion of glucose, glucose concn is 100g/L-220g/L in the substratum, corn steep liquor concentration is 5g/L-20g/L, and light calcium carbonate content is 10g/L-60g/L; 2) after light calcium carbonate exhausts in the fermenting container, stream adds sodium carbonate solution or sodium hydroxide solution, in coming and the 2-keto-D-gluconic acid that produces in the fermenting process, sodium carbonate solution concentration is 200g/L-300g/L, and concentration of sodium hydroxide solution is 200g/L-400g/L; 3) when glucose concn in the fermention medium drops between the 20g/L-100g/L, beginning adds in batches or stream adds the glucose solution that concentration is 300g/L-500g/L, the total sugaring concentration of control fermented liquid terminal point is lower than the 2g/L fermentation ends up to remaining sugar concentration between 150g/L-300g/L; This zymotechnique carries out under aerobic conditions, and control fermentation oxyty is 10%-50%, and suitable pH value is 4.2-8.0, and suitable temperature is 28 ℃-38 ℃.
Of the present invention can be that the thin strain liquid of 2-keto-D-gluconic acid can adopt Pseudomonas fluorescens, pseudomonas putida, serratia marcescens, spherical Arthrobacter, product ketone Alcaligenes or Yunnan Caulis Spatholobi Erwinia with conversion of glucose.
The present invention is producing with fermentation using bacteria in the process of 2-keto-D-gluconic acid salt, pass through technology controlling and process, make that the product of earlier fermentation is a 2-keto-D-gluconic acid calcium salt, after treating that calcium salt reaches certain content, again technology is adjusted, made that the product that generates in the later fermenting process is a 2-keto-D-gluconic acid sodium salt.The present invention has fundamentally solved the easy crystalline problem of 2-keto-D-gluconic acid calcium salt in the fermented liquid, being easy to the fermented liquid ultrafiltration technology promotes in D-saccharosonic acid and its esters production line, thereby improve the quality of D-saccharosonic acid and its esters finished product, stopped the discharging of useless calcium sulfate waste residue admittedly.
Embodiment
Embodiment 1:
Adopt 10 liters of automatically controlled fermentors, pseudomonas putida liquid strain liquid 350ml is inserted in the fermentation basic medium of 4 liters of glucose that contain 180g/L, 10g/L corn steep liquor, 40g/L light calcium carbonate, initial pH6.8, the 20 hours lime carbonate that ferments exhausts, begin stream and add the sodium carbonate solution that concentration is 220g/L, fermenting process pH is controlled at 5.2.Fermenting began the glucose solution that stream adds 400g/L in 24 hours, the total sugaring concentration of control fermented liquid terminal point 230g/L, and sugared concentration is lower than 2g/L in fermented liquid, fermentation ends.31 ℃ of whole fermentation process controlled temperature, tank pressure 0.05Mpa adjusts rotating speed and air flow and guarantees dissolved oxygen 25%.Fermented liquid terminal point 2-keto-D-gluconic acid concentration 221g/L.
Embodiment 2:
Adopt 100 liters of automatically controlled fermentors, 4 liters of Pseudomonas fluorescens liquid strain liquid are inserted in the fermentation basic medium of 45 liters of glucose that contain 180g/L, 12g/L corn steep liquor, 45g/L light calcium carbonate, initial pH6.7, the 23 hours lime carbonate that ferments exhausts, begin stream and add the sodium carbonate solution that concentration is 240g/L, fermenting process pH is controlled at 5.2.Fermenting began the glucose solution that stream adds 450g/L in 24 hours, the total sugaring concentration of control fermented liquid terminal point 250g/L, and sugared concentration is lower than 2g/L in fermented liquid, fermentation ends.29 ℃ of whole fermentation process controlled temperature, tank pressure 0.05Mpa adjusts rotating speed and air flow and guarantees dissolved oxygen 28%.Fermented liquid terminal point 2-keto-D-gluconic acid concentration 245g/L.
Embodiment 3:
Adopt 1000 liters of automatically controlled fermentors, 38 liters of Pseudomonas fluorescens liquid strain liquid are inserted in the fermentation basic medium of 380 liters of glucose that contain 180g/L, 10g/L corn steep liquor, 48g/L light calcium carbonate, initial pH6.9, the 25 hours lime carbonate that ferments exhausts, begin stream and add the sodium hydroxide solution that concentration is 260g/L, fermenting process pH is controlled at 5.2.Fermenting began the glucose solution that stream adds 500g/L in 24 hours, the total sugaring concentration of control fermented liquid terminal point 260g/L, and sugared concentration is lower than 2g/L in fermented liquid, fermentation ends.31 ℃ of whole fermentation process controlled temperature, tank pressure 0.05Mpa adjusts rotating speed and air flow and guarantees dissolved oxygen 32%.Fermented liquid terminal point 2-keto-D-gluconic acid concentration 252g/L.
The invention is not restricted to these disclosed embodiment; the present invention is with the described scope of soverlay technique scheme; and the various distortion of claim scope and equivalence variation; under the prerequisite that does not depart from technical solution of the present invention, any modification or improvement that those skilled in the art that the present invention did are realized easily all belong to the present invention's scope required for protection.
Claims (2)
- A 2-keto-D-gluconic acid mixing salt high concentration fermentation process, it is characterized in that this technology may further comprise the steps:1) in fermenting container, adds a certain amount of substratum that contains glucose, corn steep liquor and light calcium carbonate, access can be the thin strain liquid of 2-keto-D-gluconic acid with conversion of glucose, glucose concn is 100g/L-220g/L in the substratum, corn steep liquor concentration is 5g/L-20g/L, and light calcium carbonate content is 10g/L-60g/L;2) after light calcium carbonate exhausts in the fermenting container, stream adds sodium carbonate solution or sodium hydroxide solution, and in coming and the 2-keto-D-gluconic acid that produces in the fermenting process, sodium carbonate solution concentration is 200g/L-300G/L, concentration of sodium hydroxide solution are 200g/L-400g/L;3) when glucose concn in the fermention medium drops between the 20g/L-100g/L, beginning adds in batches or stream adds the glucose solution that concentration is 300g/L-500g/L, the total sugaring concentration of control fermented liquid terminal point is lower than the 2g/L fermentation ends up to remaining sugar concentration between 150g/L-300g/L;This zymotechnique carries out under aerobic conditions, and control fermentation oxyty is 10%-50%, and suitable pH value is 4.2-8.0, and suitable temperature is 28 ℃-38 ℃.
- 2, a kind of 2-keto-D-gluconic acid mixing salt according to claim 1 high concentration fermentation process, it is characterized in that described can be that the thin strain liquid of 2-keto-D-gluconic acid can adopt Pseudomonas fluorescens, pseudomonas putida, serratia marcescens, spherical Arthrobacter, product ketone Alcaligenes or Yunnan Caulis Spatholobi Erwinia with conversion of glucose.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2009101481154A CN101575621A (en) | 2009-06-22 | 2009-06-22 | High concentration fermentation process of 2-keto-D-gluconic acid mixed salt |
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| CNA2009101481154A CN101575621A (en) | 2009-06-22 | 2009-06-22 | High concentration fermentation process of 2-keto-D-gluconic acid mixed salt |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104805138A (en) * | 2015-04-07 | 2015-07-29 | 山东西王糖业有限公司 | Fermentation method of high-dry-matter sodium gluconate |
| CN104830712A (en) * | 2015-03-24 | 2015-08-12 | 山东省食品发酵工业研究设计院 | A serratia marcescens strain producing high-purity 2-keto-D-gluconic acid |
| CN105695520A (en) * | 2016-04-21 | 2016-06-22 | 江南大学 | Method for increasing yield of 2-keto-D-gluconic acid produced by fermentation process |
| CN110452862A (en) * | 2019-07-22 | 2019-11-15 | 山东大学 | A kind of pseudomonas fluorescens strain and its application |
-
2009
- 2009-06-22 CN CNA2009101481154A patent/CN101575621A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104830712A (en) * | 2015-03-24 | 2015-08-12 | 山东省食品发酵工业研究设计院 | A serratia marcescens strain producing high-purity 2-keto-D-gluconic acid |
| CN104805138A (en) * | 2015-04-07 | 2015-07-29 | 山东西王糖业有限公司 | Fermentation method of high-dry-matter sodium gluconate |
| CN105695520A (en) * | 2016-04-21 | 2016-06-22 | 江南大学 | Method for increasing yield of 2-keto-D-gluconic acid produced by fermentation process |
| CN105695520B (en) * | 2016-04-21 | 2019-12-24 | 江南大学 | Method for increasing yield of 2-keto-D-gluconic acid produced by fermentation method |
| CN110452862A (en) * | 2019-07-22 | 2019-11-15 | 山东大学 | A kind of pseudomonas fluorescens strain and its application |
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