CN106337065A - Technological method for producing sodium gluconate by enzyme method - Google Patents
Technological method for producing sodium gluconate by enzyme method Download PDFInfo
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- CN106337065A CN106337065A CN201610710741.8A CN201610710741A CN106337065A CN 106337065 A CN106337065 A CN 106337065A CN 201610710741 A CN201610710741 A CN 201610710741A CN 106337065 A CN106337065 A CN 106337065A
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- glucoseoxidase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
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Abstract
The invention provides an efficient enzyme method for producing sodium gluconate, comprising the following steps: carrying out an enzymatic reaction by adding catalase and glucose oxidase into a reaction tank containing glucose as a raw material; adding glucose oxidase to maintain enzyme activity of glucose oxidase in the reaction solution at 2000U/ml-2500U/ml, and stopping the reaction when content of glucose is lower than 3g/L; and crystallizing, separating and drying obtain a sodium gluconate finished product. In comparison with enzyme addition amount in the prior art, enzyme addition amount in the method at least can be reduced by 10%.
Description
Technical field
The present invention relates to biochemical engineering field is and in particular to a kind of process of Production by Enzymes sodium gluconate.
Background technology
Sodium gluconate calls d- sodium gluconate, and industrially purposes is quite varied, can be applicable to food, medicine, change
The industries such as work, water process, are used as high-efficient retarder, high efficiency water reducing agent etc. in Concrete Industry.
At present, the production method of sodium gluconate mainly has microbe fermentation method, electrolysis and catalytic oxidation, two enzymes method
Etc. method.Due to the mild condition of microbial method, energy-conservation is substantially widely adopted.Gluconic acid and its salt are (as gluconic acid
Sodium, calcium gluconate etc.) fermenting and producing when mainly carried out using mold fermentation, its process be fermentation tank add mass propgation base
Afterwards, access seed liquor to be fermented, the method for its ferment control mainly control cell concentration, ph, temperature, fermentation tank rotating speed,
The aspects such as dissolved oxygen concentration (do), residual sugar, concentration, the selection-breeding with excellent species can tolerate higher concentration of substrate,
But the addition due to mass propgation base and by-product bring very burden to the extraction in later stage, directly influence the matter of product
Amount and conversion ratio.
Because every batch fermentation will maintain certain cell concentration, and is often to carry out a batch fermentation to cultivate a collection of bacterium
Kind, consumption of glucose in yeast culture and sweat, so that fermentation yield is reduced.And adopt Production by Enzymes sodium gluconate, it is
By adding glucoseoxidase and catalase in glucose, glucose is urged through glucoseoxidase under certain condition
Change and react generation gluconic acid and hydrogen peroxide with oxygen and water, hydrogen peroxide resolve under catalatic acting on water with
Oxygen, gluconic acid and sodium hydroxide reaction generate sodium gluconate.Enzyme process advantage is that transformation efficiency is high, and the production time is short, produces
Product purity is high, process is simple.The current shortcoming of Production by Enzymes sodium gluconate is that in addition enzyme will produce, and enzyme is relatively costly, and
During Production by Enzymes sodium gluconate, enzyme activity can reduce, and will stop when reducing reaction to a certain extent, so enzyme addition
Larger.For example, taking current this area Novozymes Company of Production by Enzymes dominant company as a example, it is Fructus Vitis viniferae that the said firm produces enzyme addition
Carbohydrate oxidase 3.5kg/t dry, catalase-3 .5kg/t dry.
In sum, current this area lacks the optimization method of Production by Enzymes sodium gluconate, that is, in the urgent need to a kind of section
Save enzyme addition, reduce production cost, the method simultaneously again improving throughput rate.
Content of the invention
In view of this, the invention provides a kind of method of efficient Production by Enzymes sodium gluconate, comprise the following steps:
1), add glucose in retort;
2), catalase and glucoseoxidase is added to carry out enzymatic reaction in retort;
3), stream plus glucoseoxidase, make the enzyme activity of glucoseoxidase in reactant liquor maintain 2000u/ml-2500u/
Ml, terminates to react when glucose content is less than 3g/l;
4) enzyme denaturing, by the sodium gluconate obtaining decolouring, filters, crystallizes, separating, being dried, obtaining sodium gluconate finished product.
Preferably, in the method for the present invention efficient Production by Enzymes sodium gluconate, described step 1) described in glucose be
300g/l-350g/l.
Preferably, in the method for the present invention efficient Production by Enzymes sodium gluconate, described step 1) in be also added into froth breaking
Agent.
Preferably, in the method for the present invention efficient Production by Enzymes sodium gluconate, described step 2) the middle described mistake adding
The enzyme activity of hydrogen oxide enzyme is 37.5 ten thousand u/ml, and the enzyme activity of described glucoseoxidase is 873.6 ten thousand u/ml.
Preferably, in the method for the present invention efficient Production by Enzymes sodium gluconate, described step 2) condition be stream plus
35% sodium hydroxide controls ph5.5-6.0, controls rotating speed and air quantity to maintain dissolved oxygen 30%-40% in tank.
Preferably, in the method for the present invention efficient Production by Enzymes sodium gluconate, described step 3) middle control Fructus Vitis viniferae glycosyloxy
The enzyme activity changing enzyme is to start when enzyme activity is reduced to 2100u/ml-2600u/ml in the method for 2000u/ml-2500u/ml
Stream plus glucoseoxidase (87.36 ten thousand u/ml).
Preferably, in the method for the present invention efficient Production by Enzymes sodium gluconate, described step 4) described in enzyme denaturing bar
Part is the 80 DEG C of enzyme denaturing 30min that heat up.
Preferably, in the method for the present invention efficient Production by Enzymes sodium gluconate, described step 4) described in decolour for making
Use activated carbon decolorizing.
Preferably, in the method for the present invention efficient Production by Enzymes sodium gluconate, described step 4) described in the side filtered
Method is the plate-and-frame filtration removal of impurity;The method of described crystallization is concentrated into more than 70% content, crystallisation by cooling for 105-115 DEG C;Described
Crystal is separated with liquid by detached method for seperator;The method of described drying is fluid bed drying.
From the foregoing, it will be observed that the method for the present invention efficient Production by Enzymes sodium gluconate, at least have the advantage that
1) method of the present invention can greatly save enzyme addition, reduces production cost;
2) simultaneously, throughput rate can be improved again.
3) method of the present invention for prior art enzyme addition (as glucoseoxidase 3.5kg/t dry, mistake
Hydrogen oxide enzyme 3.5kg/t dry), at least can reduce by 10%.
Specific embodiment
Further technical scheme is illustrated it should be understood that being only this below below by way of specific embodiment
Bright exemplary illustration, is not limited to the protection domain of the claims in the present invention.
Embodiment 1:
The first step, adds 30l reactant liquor wherein glucose content 300g/l in 50l tank, adds the defoamer of 6ml;
Second step, is separately added into catalase-3 1.5ml (addition is 3.5kg/t dry), glucoseoxidase in tank
10ml (in tank enzyme activity be more than 2500u/ml) starts to react, and flows the sodium hydroxide Jia 35% and controls ph5.5-6.0, control rotating speed with
Air quantity maintains dissolved oxygen 30%-40% in tank.
3rd step, stream plus glucoseoxidase, the content of glucoseoxidase in detection reactant liquor, when enzyme activity is reduced to
Start stream when 2100u/ml plus glucoseoxidase (87.36 ten thousand u/ml) makes the enzyme activity of glucoseoxidase in reactant liquor tie up
Hold in 2000u/ml.Terminate to react when glucose content is less than 3g/l.Reaction duration 20 hours, glucoseoxidase is total
Usage amount is 28ml (being equivalent to addition is 3.1kg/t dry).
4th step, enzyme denaturing, decolouring: heat up 80 DEG C of enzyme denaturing 30min, activated carbon decolorizing.
5th step, filtration: the plate-and-frame filtration removal of impurity.
6th step, crystallization: 105-115 DEG C is concentrated into mass content more than 70%, crystallisation by cooling.
7th step, separation, drying: crystal is separated by seperator with liquid, then fluid bed drying, obtain sodium gluconate
Product.Sodium gluconate purity is 99.4%, and yield is 96.2%;With the comparative example addition (glucose mentioned in background technology
Oxidase 3.5kg/t dry) to compare, glucoseoxidase addition saves 11% (addition is 3.1kg/t dry).
Embodiment 2:
The first step, adds 30l reactant liquor wherein glucose content 350g/l in 50l tank, adds the defoamer of 6ml;
Second step, is separately added into catalase-3 6.75m1 (37.5 ten thousand u/m1), glucoseoxidase 10ml (tank in tank
Interior enzyme activity is more than 2500u/m1) start to react, flow the sodium hydroxide Jia 35% and control ph5.5-6.0, control rotating speed and air quantity dimension
Hold dissolved oxygen 30%-40% in tank.
3rd step, stream plus glucoseoxidase, the content of glucoseoxidase in detection reactant liquor, when enzyme activity is reduced to
Start stream when 2600u/ml plus glucoseoxidase (87.36 ten thousand u/ml) makes the enzyme activity of glucoseoxidase in reactant liquor tie up
Hold in 2500u/ml.Terminate to react when glucose content is less than 3g/l.Reaction duration 18.5 hours, glucoseoxidase
Total usage amount is 31ml (addition is 2.95kg/t dry).
4th step, enzyme denaturing, decolouring: heat up 80 DEG C of enzyme denaturing 30min, activated carbon decolorizing.
5th step, filtration: the plate-and-frame filtration removal of impurity.
6th step, crystallization: 105-115 DEG C is concentrated into mass content more than 70%, crystallisation by cooling.
7th step, separation, drying: crystal is separated by seperator with liquid, then fluid bed drying, obtain sodium gluconate
Product.Sodium gluconate purity is 99.5%, and yield is 96.8%, and glucoseoxidase addition saves 15.65% (with background skill
The comparative example addition 3.5kg/t dry mentioned in art is compared, and the total usage amount of glucoseoxidase is 2.95kg/t dry).
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of method of efficient Production by Enzymes sodium gluconate, comprises the following steps:
1), add glucose in retort;
2), catalase and glucoseoxidase is added to carry out enzymatic reaction in retort;
3), stream plus glucoseoxidase, make the enzyme activity of glucoseoxidase in reactant liquor maintain 2000u/ml-2500u/ml,
Terminate to react when glucose content is less than 3g/l;
4) enzyme denaturing, by the sodium gluconate obtaining decolouring, filters, crystallizes, separating, being dried, obtaining sodium gluconate finished product.
2. method according to claim 1 is it is characterised in that described step 1) described in glucose be 300g/l-350g/
l.
3. method according to claim 1 is it is characterised in that described step 1) in be also added into defoamer.
4. method according to claim 3 is it is characterised in that the amount of described defoamer is 0.2 ‰ weight.
5. method according to claim 1 is it is characterised in that described step 2) in the described catalatic enzyme that adds
Living is 37.5 ten thousand u/ml, and the enzyme activity of described glucoseoxidase is 873.6 ten thousand u/ml.
6. method according to claim 1 is it is characterised in that described step 2) condition be the sodium hydroxide that stream Jia 35%
Control ph5.5-6.0, control rotating speed and air quantity to maintain dissolved oxygen 30%-40% in tank.
7. method according to claim 1 is it is characterised in that described step 3) in control the enzyme activity of glucoseoxidase to exist
The method of 2000u/ml-2500u/ml is: starts stream when enzyme activity is reduced to 2100u/ml-2600u/ml with Fructus Vitis viniferae glycosyloxy
Change enzyme.
8. method according to claim 1 is it is characterised in that described step 4) described in the condition of enzyme denaturing be to heat up 80 DEG C
Enzyme denaturing 30min.
9. method according to claim 1 is it is characterised in that described step 4) described in decolour be to be taken off using activated carbon
Color.
10. method according to claim 1 is it is characterised in that described step 4) described in the method that filters be sheet frame mistake
The method filtering crystallization described in impurity is concentrated into more than 70% content, crystallisation by cooling for 105-115 DEG C;Described detached method is
Crystal is separated by seperator with liquid;The method of described drying is fluid bed drying.
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Cited By (7)
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CN106868065A (en) * | 2017-03-31 | 2017-06-20 | 山东福洋生物科技有限公司 | The continuous producing method and device of a kind of sodium gluconate |
CN107012176A (en) * | 2017-04-21 | 2017-08-04 | 山东大学 | A kind of method of the conversion production sodium gluconate by material, enzyme method of cellulose biomass |
CN109022506A (en) * | 2018-08-17 | 2018-12-18 | 青岛中科潮生生物技术有限公司 | The method that sodium gluconate is prepared using lignocellulosic |
CN112029806A (en) * | 2020-09-10 | 2020-12-04 | 河北乐开节能科技股份有限公司 | Method for producing sodium gluconate through normal-pressure enzymatic oxidation |
CN113249413A (en) * | 2020-07-08 | 2021-08-13 | 山东福洋生物科技股份有限公司 | Method for producing sodium gluconate by recycling aspergillus niger mycelia |
CN116515914A (en) * | 2023-06-25 | 2023-08-01 | 山东福洋生物科技股份有限公司 | Production method for improving quality of sodium gluconate finished product |
CN116855550A (en) * | 2023-09-04 | 2023-10-10 | 山东福洋生物科技股份有限公司 | Method for producing sodium gluconate by enzyme method |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106868065A (en) * | 2017-03-31 | 2017-06-20 | 山东福洋生物科技有限公司 | The continuous producing method and device of a kind of sodium gluconate |
CN107012176A (en) * | 2017-04-21 | 2017-08-04 | 山东大学 | A kind of method of the conversion production sodium gluconate by material, enzyme method of cellulose biomass |
CN107012176B (en) * | 2017-04-21 | 2021-01-15 | 山东大学 | Method for producing sodium gluconate by using cellulose biomass as raw material through enzymatic conversion |
CN109022506A (en) * | 2018-08-17 | 2018-12-18 | 青岛中科潮生生物技术有限公司 | The method that sodium gluconate is prepared using lignocellulosic |
CN113249413A (en) * | 2020-07-08 | 2021-08-13 | 山东福洋生物科技股份有限公司 | Method for producing sodium gluconate by recycling aspergillus niger mycelia |
CN112029806A (en) * | 2020-09-10 | 2020-12-04 | 河北乐开节能科技股份有限公司 | Method for producing sodium gluconate through normal-pressure enzymatic oxidation |
CN116515914A (en) * | 2023-06-25 | 2023-08-01 | 山东福洋生物科技股份有限公司 | Production method for improving quality of sodium gluconate finished product |
CN116855550A (en) * | 2023-09-04 | 2023-10-10 | 山东福洋生物科技股份有限公司 | Method for producing sodium gluconate by enzyme method |
CN116855550B (en) * | 2023-09-04 | 2023-11-17 | 山东福洋生物科技股份有限公司 | Method for producing sodium gluconate by enzyme method |
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