CN113621507A - Full-automatic carrying and independent temperature and CO control2Multi-culture-cabin incubator with concentration - Google Patents
Full-automatic carrying and independent temperature and CO control2Multi-culture-cabin incubator with concentration Download PDFInfo
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- CN113621507A CN113621507A CN202110784397.8A CN202110784397A CN113621507A CN 113621507 A CN113621507 A CN 113621507A CN 202110784397 A CN202110784397 A CN 202110784397A CN 113621507 A CN113621507 A CN 113621507A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/44—Multiple separable units; Modules
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
Abstract
The invention provides a full-automatic conveying device capable of independently controlling temperature and CO2A multi-culture-cabin incubator with concentration. The invention comprises a culture box body and a plurality of culture cabins arranged in the culture box body, wherein each culture cabin is in an independent culture space in the culture box, each culture cabin can be automatically and independently carried and output through an instruction sent by a control system, and each culture cabin is matched with a temperature sensor and CO2Concentration sensor, control system based on each temperature sensor and CO2The data transmitted by the concentration sensor respectively control the temperature and CO of each culture cabin2And (4) concentration. The incubator can ensure that 8 culture cabins carry out cell culture work simultaneously, and cultured cells can not be subjected to cross contamination and influence. Can be independently decontaminated, and avoids cell culture andthe defect that the staining can not be carried out simultaneously is overcome, and the cell culture efficiency is effectively improved. Simultaneously ensuring the temperature and CO of other culture cabins in the moving-out process of each culture cabin2The concentration was not changed. Effectively improves the effect of cell culture.
Description
Technical Field
The invention relates to the technical field of cell modulation, in particular to a full-automatic conveying device capable of independently controlling temperature and CO2A multi-culture-cabin incubator with concentration.
Background
CO2The incubator is an advanced instrument for culturing cells, tissues and bacteria, is a key device necessary for developing immunology, oncology, genetics and bioengineering, and is CO2The incubator is improved on the basis of a common incubator and mainly can be added with CO2So as to meet the environment required by the culture of microorganisms. CO widely used in the market at present2The incubator is a single cavity, and only one incubator can be used in the culture processThe culture is carried out integrally, and the condition that culture bodies are mutually crossed and polluted is easy to occur when multi-body culture is carried out, so that the culture effect is influenced; and single cavity CO2When the incubator is used for multi-body culture, CO is generated in the operation process2The concentration and the temperature can be greatly changed, and the culture effect of other cultures is easily influenced; and single cavity CO2When the culture box is used for decontamination, all culture operations need to be stopped, and decontamination cannot be carried out at the same time of culture.
Disclosure of Invention
In view of the above-mentioned problems, it is an object of the present invention to provide a fully automatic transportation system capable of independently controlling temperature and CO2A multi-culture-cabin incubator with concentration. The technical means adopted by the invention are as follows:
full-automatic carrying and independent temperature and CO control2The multi-culture-compartment incubator of concentration comprises: the culture box body and the culture cabins arranged in the culture box body are arranged, each culture cabin is in an independent culture space in the culture box body, each culture cabin can be automatically and independently carried and output through instructions sent by the control system, and each culture cabin is matched with a temperature sensor and CO2Concentration sensor, control system based on each temperature sensor and CO2The data transmitted by the concentration sensor respectively control the temperature and CO of each culture cabin2And (4) concentration.
Further, the culture cabin comprises a culture cabin cavity and a plurality of annular interfaces arranged on the culture cabin cavity, and the annular interfaces are used for being in butt joint with the temperature sensor and the CO2Concentration sensor, CO2Gas input interface and CO2And a gas outlet interface.
Further, the temperature sensor and the CO are completed by controlling the lifting of the platform2Concentration sensor, CO2Gas input interface, CO2The separation and butt joint of the gas discharge interface and the annular interface of the culture cabin, specifically, when the culture cabin is moved out, the control platform is lifted upwards, the temperature sensor and the CO are detected2Concentration sensor, CO2Gas input interface, CO2The gas discharge interface is separated from the annular interface of the culture cabin, so that the culture cabin is not influenced to be moved out;when the culture cabin is moved into the culture cabin, the control platform descends downwards, and the temperature sensor and the CO are connected with the control platform2Concentration sensor, CO2Gas input interface, CO2The gas outlet interface is in butt joint with the annular interface of the culture cabin for adjusting the temperature and CO in the culture cabin2The concentration was monitored.
Furthermore, the annular interface on the control platform is provided with the silica gel pad, and through the decurrent pressure of control platform, guarantee that the annular interface of control platform and cultivation cabin closely docks, guarantee that the cultivation cabin is independent cultivation space.
Furthermore, each culture cabin is automatically and independently conveyed and output through a manipulator.
Further, the culture chamber is filled with CO2The concentration sensor detects CO in the culture chamber2Actual value of concentration, when CO2When the concentration is lower than the set concentration, CO is turned on2Air inlet solenoid valve and CO2Gas exhaust solenoid valve, stored CO of preset concentration2Gas enters the culture cabin under the pressure of the air pump to ensure that CO in the culture cabin2The concentration reaches the set concentration; when culturing CO in the cabin2After the concentration reaches the set concentration, CO is closed2Air inlet solenoid valve and CO2Air exhaust solenoid valve.
Further, the set concentration is CO2When the concentration is lower than the set concentration, CO2Mixing with air in air tank, and respectively feeding mixed CO with set concentration into each culture cabin via air pump2And (4) mixing the gases.
Further, the temperature in the culture cabin is detected by a temperature sensor arranged in the culture cabin, and when the temperature in the culture cabin is higher than or equal to a set temperature, CO is started2Air inlet solenoid valve and CO2Gas exhaust solenoid valve, CO of set concentration2The gas enters the culture cabin under the pressure of the air pump and passes through the CO with the set concentration of the temperature value at the room temperature2The gas enables the temperature in the culture cabin to reach the set temperature; when the temperature in the culture chamber is lower than the set temperature, CO is turned off2Air inlet solenoid valve and CO2The gas exhaust electromagnetic valve is controlled by the set temperature in the incubatorAnd (4) carrying out radiant heating on the culture cabin to enable the temperature in the culture cabin to reach the set temperature.
Further, the set temperature was 37 ℃.
Further, CO2The concentration control comprises the following steps: when the culture chamber is moved in, the CO with the set concentration is quickly filled in2Gas, which converts the air in the equipment into CO with set concentration in 5 seconds2Gas; when culturing CO in the cabin2The control method for continuous gas consumption comprises the following steps: CO in the culture chamber2Concentration of CO less than or equal to the set value2At a concentration of-0.15%, CO2Air inlet solenoid valve and CO2The gas exhaust solenoid valve is opened and maintained at the same time; CO in the culture chamber2Concentration of CO not less than set2And when the concentration is higher than the preset temperature, closing the culture chamber after the time delay of 5 seconds, wherein the temperature in the culture chamber is less than or equal to the set temperature plus 0.1 ℃.
Due to the adoption of the technical scheme, the full-automatic conveying device provided by the invention can be used for carrying out full-automatic conveying and independently controlling the temperature and CO2A multi-culture-cabin incubator with concentration. This incubator can guarantee that 8 cultivate the cabin and carry out cell culture work simultaneously, and the cell of cultivateing can not carry out cross contamination and influence. Can be independently decontaminated, avoids the defect that cell culture and decontamination can not be carried out simultaneously, and effectively improves the efficiency of cell culture. Ensure the temperature and CO of other culture cabins in the process of moving out each culture cabin2The concentration was not changed. Effectively improves the effect of cell culture.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 shows a fully automated transport with independent temperature and CO control according to the present invention2Structural block diagram of a multi-culture-cabin incubator of concentration.
FIG. 2 is a view showing the structure of a culture chamber of the present invention.
FIG. 3 is a block diagram of a control platform according to the present invention.
In the figure: 1. a culture box body; 2. a culture compartment; 3. a delivery group; 4. a control platform; 5. an electromagnetic valve; 21. a culture compartment cavity; 22. a ring interface; 41. a platform body; 42. a temperature sensor; 43. CO22A concentration sensor; 44. a transmission set; 45. a silica gel pad.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in FIG. 1, the embodiment of the invention discloses a fully automatic conveying device capable of independently controlling temperature and CO2The many culture compartments incubator of concentration, this incubator is in a confined space in the work, includes: cultivate box 1 and arrange its inside a plurality of cultivation cabins 2 in, every cultivation cabin all is in independent cultivation space in the incubator, and the instruction that every cultivation cabin accessible control system sent is through the automatic independent transport of transmission group 3 and output, in this embodiment, transmission group optional step motor and manipulator are in order to accomplish the automation of cultivateing the cabin and take out. Each culture cabin is matched with a temperature sensor and CO2Concentration sensor, control system based on each temperature sensor and CO2The data transmitted by the concentration sensor respectively control the temperature and CO of each culture cabin2And (4) concentration. When one culture cabin is in the process of carrying and outputting, no influence is caused on the cell culture of other culture cabins, and the cells cultured in each culture cabin are ensured not to be subjected to cross contamination and influence. In this example, the temperature of the center of the incubator was controlled to a set value of-0.3 ℃ during the operation, the temperature of each culture chamber was controlled to 37 ℃ (or a set temperature), and CO was added to the incubator2The concentration is controlled at 5% (or set temperature), eachThe temperature and CO in other culture cabins cannot be influenced in the moving process of the culture cabin2The change in the concentration value.
As shown in FIG. 2, the culture compartment comprises a culture compartment cavity 21 and a plurality of annular interfaces 22 arranged on the culture compartment cavity, wherein the annular interfaces are used for being connected with a temperature sensor and a CO2Concentration sensor, CO2Gas input interface and CO2And a gas outlet interface.
As shown in FIG. 3, the temperature sensor, CO, is completed by controlling the elevation of the platform 42Concentration sensor, CO2Gas input interface, CO2The gas outlet interface is separated from and connected with the annular interface of the culture cabin, and the control platform 4 comprises a platform body 41, and a temperature sensor 42 and CO which are arranged on the platform body2 A concentration sensor 43 for controlling the lifting of the platform body through a transmission set 44, specifically, when the culture chamber is moved out, the control platform is lifted upwards, and the temperature sensor and CO are detected2Concentration sensor, CO2Gas input interface, CO2The gas discharge interface is separated from the annular interface of the culture cabin, so that the culture cabin is not influenced to be moved out; when the culture cabin is moved into the culture cabin, the control platform descends downwards, and the temperature sensor and the CO are connected with the control platform2Concentration sensor, CO2Gas input interface, CO2The gas outlet interface is in butt joint with the annular interface of the culture cabin for adjusting the temperature and CO in the culture cabin2The concentration was monitored.
The annular interface on the control platform is provided with silica gel pad 45, through the decurrent pressure of control platform, guarantees that the annular interface of control platform and cultivation cabin closely docks, guarantees to cultivate the cabin and be independent cultivation space.
In this embodiment, the transmission set can be a manipulator, and each culture chamber is automatically and independently transported and output by the manipulator.
CO passing through culture cabin2The concentration sensor detects CO in the culture chamber2Actual value of concentration, when CO2When the concentration is lower than the set concentration, CO is turned on2Air inlet solenoid valve 5 and CO2Gas exhaust solenoid valve 5, stored CO of preset concentration2The gas enters the culture cabin under the pressure of the air pumpIn the culture chamber, CO is enabled to be in the culture chamber2The concentration reaches the set concentration; when culturing CO in the cabin2After the concentration reaches the set concentration, CO is closed2Air inlet solenoid valve and CO2Air exhaust solenoid valve. In this example, the set concentration is CO2Concentration less than 5%, CO2Mixing with air in air tank, and respectively introducing mixed 5% CO into each culture chamber via air pump2。
The temperature in the culture cabin is detected by a temperature sensor arranged in the culture cabin, and when the temperature in the culture cabin is higher than or equal to a set temperature, CO is started2Air inlet solenoid valve and CO2Gas exhaust solenoid valve, CO of set concentration2The gas enters the culture cabin under the pressure of the air pump and passes through the CO with the set concentration of the temperature value at the room temperature2The gas enables the temperature in the culture cabin to reach the set temperature; when the temperature in the culture chamber is lower than the set temperature, CO is turned off2Air inlet solenoid valve and CO2The gas exhaust electromagnetic valve is used for carrying out radiant heating on the culture cabin through the set temperature in the culture box, so that the temperature in the culture cabin reaches the set temperature. In this example, the set temperature was 37 ℃.
，CO2The concentration control comprises the following steps: when the culture chamber is moved in, the CO with the set concentration is quickly filled in2Gas, which converts the air in the equipment into CO with set concentration in 5 seconds2Gas; the control method when the CO2 gas in the culture cabin is continuously consumed is as follows: CO in the culture chamber2Concentration of CO less than or equal to the set value2At a concentration of-0.15%, CO2Air inlet solenoid valve and CO2The gas exhaust solenoid valve is opened and maintained at the same time; CO in the culture chamber2Concentration of CO not less than set2And when the concentration is higher than the preset temperature, closing the culture chamber after the time delay of 5 seconds, wherein the temperature in the culture chamber is less than or equal to the set temperature plus 0.1 ℃. In this example, the concentration was set to 5%.
When the dye is removed, the culture cabin which is not in the culture state can be randomly selected, and the electromagnetic valve CO corresponding to the selected culture cabin2The air inlet electromagnetic valve is switched to H2O2Supply valve with CO2The gas exhaust solenoid valve opens, so that H2O2Gas circulates in the culture cabin to completely remove the stain; when the culture chamber is used for the culture, the culture chamber has no influence on other culture chambers during culture.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. Full-automatic carrying and independent temperature and CO control2The multi-culture-cabin incubator with the concentration is characterized by comprising an incubator body and a plurality of culture cabins arranged in the incubator body, wherein each culture cabin is in an independent culture space in the incubator, each culture cabin can be automatically and independently carried and output through an instruction sent by a control system, and each culture cabin is matched with a temperature sensor and CO2Concentration sensor, control system based on each temperature sensor and CO2The data transmitted by the concentration sensor respectively control the temperature and CO of each culture cabin2And (4) concentration.
2. The fully automated handling of claim 1 with independent temperature and CO control2The multi-culture-cabin incubator with the concentration is characterized in that the culture cabin comprises a culture cabin cavity and a plurality of annular interfaces arranged on the culture cabin cavity, and the annular interfaces are used for butting a temperature sensor and CO2Concentration sensor, CO2Gas input interface and CO2And a gas outlet interface.
3. The fully automated handling of claim 2 with independent temperature and CO control2The multi-culture-cabin incubator is characterized in that the temperature sensor and CO are completed by controlling the lifting of the platform2Concentration sensor, CO2Gas transmissionInlet port, CO2The separation and butt joint of the gas discharge interface and the annular interface of the culture cabin, specifically, when the culture cabin is moved out, the control platform is lifted upwards, the temperature sensor and the CO are detected2Concentration sensor, CO2Gas input interface, CO2The gas discharge interface is separated from the annular interface of the culture cabin, so that the culture cabin is not influenced to be moved out; when the culture cabin is moved into the culture cabin, the control platform descends downwards, and the temperature sensor and the CO are connected with the control platform2Concentration sensor, CO2Gas input interface, CO2The gas outlet interface is in butt joint with the annular interface of the culture cabin for adjusting the temperature and CO in the culture cabin2The concentration was monitored.
4. The fully automated handling of claim 3 with independent temperature and CO control2The multi-culture-cabin incubator is characterized in that a silica gel pad is arranged on an annular interface on a control platform, the control platform is enabled to be in tight butt joint with the annular interface of a culture cabin through downward pressure of the control platform, and the culture cabin is enabled to be an independent culture space.
5. The fully automated handling of claim 1 with independent temperature and CO control2The multi-culture-cabin incubator is characterized in that each culture cabin is automatically and independently carried and output through a manipulator.
6. The fully automated handling of claim 1 with independent temperature and CO control2The multi-culture-cabin incubator is characterized in that the culture cabins pass through set CO2The concentration sensor detects CO in the culture chamber2Actual value of concentration, when CO2When the concentration is lower than the set concentration, CO is turned on2Air inlet solenoid valve and CO2Gas exhaust solenoid valve, stored CO of preset concentration2Gas enters the culture cabin under the pressure of the air pump to ensure that CO in the culture cabin2The concentration reaches the set concentration; when culturing CO in the cabin2After the concentration reaches the set concentration, CO is closed2Air inlet solenoid valve and CO2Air exhaust solenoid valve.
7. The fully automated handling of claim 6 with independent temperature and CO control2The multi-culture-cabin incubator is characterized in that the set concentration is CO2When the concentration is lower than the set concentration, CO2Mixing with air in air tank, and respectively feeding mixed CO with set concentration into each culture cabin via air pump2And (4) mixing the gases.
8. The fully automated handling of claim 1 with independent temperature and CO control2The multi-culture-cabin incubator is characterized in that the temperature in the culture cabin is detected by a temperature sensor arranged in the culture cabin, and when the temperature in the culture cabin is higher than or equal to a set temperature, CO is started2Air inlet solenoid valve and CO2Gas exhaust solenoid valve, CO of set concentration2The gas enters the culture cabin under the pressure of the air pump and passes through the CO with the set concentration of the temperature value at the room temperature2The gas enables the temperature in the culture cabin to reach the set temperature; when the temperature in the culture chamber is lower than the set temperature, CO is turned off2Air inlet solenoid valve and CO2The gas exhaust electromagnetic valve is used for carrying out radiant heating on the culture cabin through the set temperature in the culture box, so that the temperature in the culture cabin reaches the set temperature.
9. The fully automated handling of claim 8 with independent temperature and CO control2The multi-culture-cabin incubator is characterized in that the set temperature is 37 ℃.
10. The fully automated handling of any of claims 1 to 9 with independent temperature and CO control2The multi-culture-cabin incubator is characterized in that CO2The concentration control comprises the following steps: when the culture chamber is moved in, the CO with the set concentration is quickly filled in2Mixing gas, and converting air in the equipment into CO with set concentration in 5 seconds2Mixing the gas; when culturing CO in the cabin2The control method during continuous consumption comprises the following steps:CO in the culture chamber2Concentration of CO less than or equal to the set value2At a concentration of-0.15%, CO2Air inlet solenoid valve and CO2The gas exhaust solenoid valve is opened and maintained at the same time; CO in the culture chamber2Concentration of CO not less than set2And when the concentration is higher than the preset temperature, closing the culture chamber after the time delay of 5 seconds, wherein the temperature in the culture chamber is less than or equal to the set temperature plus 0.1 ℃.
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| CN202110784397.8A CN113621507A (en) | 2021-07-12 | 2021-07-12 | Full-automatic carrying and independent temperature and CO control2Multi-culture-cabin incubator with concentration |
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Cited By (3)
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| CN114196538A (en) * | 2021-12-02 | 2022-03-18 | 冰山松洋生物科技(大连)有限公司 | Multi-culture-cabin CO2Concentration independent adjusting method and gas circuit system |
| CN114369526A (en) * | 2022-01-26 | 2022-04-19 | 石家庄学院 | Many floras isolated culture system |
| CN114106990B (en) * | 2021-12-02 | 2023-08-29 | 冰山松洋生物科技(大连)有限公司 | Air path system and air path control method of honeycomb carbon dioxide incubator |
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