CN114106990B - Air path system and air path control method of honeycomb carbon dioxide incubator - Google Patents
Air path system and air path control method of honeycomb carbon dioxide incubator Download PDFInfo
- Publication number
- CN114106990B CN114106990B CN202111460176.1A CN202111460176A CN114106990B CN 114106990 B CN114106990 B CN 114106990B CN 202111460176 A CN202111460176 A CN 202111460176A CN 114106990 B CN114106990 B CN 114106990B
- Authority
- CN
- China
- Prior art keywords
- gas
- air
- unit
- incubator
- electromagnetic valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/40—Manifolds; Distribution pieces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a gas circuit system and a gas circuit control method of a honeycomb carbon dioxide incubator. The system includes CO 2 A gas preparation unit; vaporous H 2 O 2 A generating unit; the gas circuit switching unit; an incubator body unit and an exhaust unit. When the culture cabin works, the CO is controlled by the gas circuit switching unit 2 Gas is produced from the CO 2 The gas preparation unit enters the culture cabin and simultaneously blocks the vaporous state H 2 O 2 Entering; when the sterilizing is carried out, the steam state H is discharged by the piezoelectric pump 2 O 2 From the vapour state H 2 O 2 The generating unit is led into the main body unit of the incubator, and then the gas state H is controlled by the gas circuit switching unit 2 O 2 Enter the culture cabin and simultaneously block CO 2 And after the gas enters and reaches the disinfection time requirement, opening the exhaust electromagnetic valve.
Description
Technical Field
The invention relates to an incubator system, in particular to a gas circuit system and a gas circuit control method of a honeycomb carbon dioxide incubator.
Background
With the increasing development of related fields such as biology, regenerative medicine, epidemic prevention and control in recent years, the market demand for a carbon dioxide incubator capable of stably and efficiently culturing cells is increasing. In order to better meet the market demand, the honeycomb carbon dioxide incubator can cultivate a plurality of different batches at the same time. Cell culture pair CO 2 The concentration and temperature requirements are very strict, when the temperature and CO 2 When the concentration is destroyed, the culture efficiency of the cells is seriously affected, and the death of the cells is most seriously caused. The cell culture needs to sterilize and disinfect the interior of the incubator regularly, and in equipment with high requirement on environmental cleanliness, a hydrogen peroxide sterilizing device is most commonly used, and the common sterilizing and disinfecting time is 6-24 hours. Since different cell culture periods are different, if the cabin is opened for long-time sterilization and disinfection, the influence on cell culture is self-evident. And the maximum utilization of the cell incubator can not be realized without sterilization and disinfection.
Disclosure of Invention
To control incubator CO 2 The invention provides a gas circuit system and a gas circuit control method of a honeycomb carbon dioxide incubator. The control loop designed by the invention can accurately control CO 2 Concentration and can realize CO 2 And (3) rapidly switching the concentration and sterilization of hydrogen peroxide.
The invention adopts the following technical means:
a gas circuit system of a honeycomb carbon dioxide incubator, comprising:
CO 2 a gas preparation unit, the CO 2 The gas preparation unit mainly comprises CO 2 Gas cylinder, air pump, gas storage tank, said CO 2 The air cylinder and the air pump are respectively connected with the air storage tank through one air inlet pipeline, and two-position two-way electromagnetic valves are arranged on the air inlet pipeline;
vaporous H 2 O 2 A generation unit for generating the steam state H 2 O 2 The generating unit comprises a hydrogen peroxide generating device and a piezoelectric pump, and the steam state H is 2 O 2 The generating unit is connected with the air inlet of the incubator main body unit;
the gas circuit switching unit, the first air inlet of the gas circuit switching unit and the CO 2 The air outlet of the air preparation unit is communicated with the air outlet of the incubator main body unit through a first air passage pipeline, the second air inlet is communicated with the air outlet of the incubator main body unit through a second air passage pipeline, the air outlet of the air passage switching unit is communicated with the air inlet of the incubator main body unit, the air outlet of the incubator is communicated with the air exhaust unit through an air exhaust pipeline, and the air exhaust pipeline is provided with two-position two-way electromagnetic valves;
the incubator comprises an incubator main body unit, a plurality of incubator control units and a control unit, wherein the incubator main body unit comprises a plurality of incubator cabins;
the exhaust unit is an exhaust electromagnetic valve;
when the culture cabin works, the CO is controlled by the gas circuit switching unit 2 Gas is produced from the CO 2 The gas preparation unit enters the culture cabin and simultaneously blocks the vaporous state H 2 O 2 Entering; when the sterilizing is carried out, the steam state H is discharged by the piezoelectric pump 2 O 2 From the vapour state H 2 O 2 The generating unit is led into the main body unit of the incubator, and then the gas state H is controlled by the gas circuit switching unit 2 O 2 Enter the culture cabin and simultaneously block CO 2 And after the gas enters and reaches the disinfection time requirement, opening the exhaust electromagnetic valve.
Further, the saidThe exhaust unit also comprises H connected behind the exhaust electromagnetic valve 2 O 2 A decomposer.
Further, the CO 2 The gas preparation unit is connected with CO 2 The busbar is communicated with the first air passage pipeline; the air outlet of the incubator main body unit is provided with an H 2 O 2 The busbar is communicated with the second gas path pipeline;
the gas circuit switching unit comprises a plurality of two-position three-way electromagnetic valves which are arranged in parallel, and a first gas inlet and CO of each two-position three-way electromagnetic valve 2 One branch of the busbar output is communicated with the second air inlet and H 2 O 2 And a branch of the busbar output is communicated, and the air outlet of each two-position three-way electromagnetic valve is communicated with a culture cabin.
Further, the CO 2 A two-position two-way electromagnetic valve is arranged between one branch of the busbar output and the corresponding two-position three-way electromagnetic valve.
Further, CO 2 The air inlet pipeline for connecting and communicating the air cylinder with the air storage tank is also provided with CO 2 A filter valve, a filter, and a gas flow rate adjustment meter;
an air filter valve, a filter and a gas flow regulating meter are also arranged on an air inlet pipeline connected and communicated with the air pump and the air storage tank.
The invention also discloses a gas path control method of the honeycomb carbon dioxide incubator, which is realized based on any gas path system and comprises the following steps:
when the culture cabin works, CO 2 The electromagnetic valve of the corresponding air inlet passage is connected after the busbar, and the two-position three-way valve is CO 2 Gas entering, vapour state H 2 O 2 In a blocking state, gas flows into the corresponding culture cabins, and meanwhile, the flow rate adjusting meter controls the flow rate of the gas correspondingly according to different numbers of culture cabins;
when H is 2 O 2 In the case of killing, first pass through H 2 O 2 The generator will be liquid H 2 O 2 Conversion to the vapour state H 2 O 2 Then power is provided by the piezoelectric pump, at the moment, the air inlet electromagnetic valve of the corresponding culture cabin is closed,and H is 2 O 2 The two-position three-way electromagnetic valve behind the busbar is CO 2 Gas blocking, vaporous H 2 O 2 When passing state and reaching the disinfection time requirement, the total exhaust battery valve is opened, and the steam state H 2 O 2 Enter H 2 O 2 Decomposing in a decomposer.
Further, when the culture cabin works, the method also comprises the step of configuring the CO with the required concentration 2 Specifically comprises the following steps:
obtaining CO requiring preparation 2 Concentration;
turning on CO 2 Two-position two-way electromagnetic valves arranged on the air inlet pipeline and the air inlet pipeline, and simultaneously inputting CO into the air storage tank 2 Gas and air, and through CO 2 Concentration sensor feedback CO 2 Concentration variation of (2);
when CO 2 When the concentration of (2) reaches the adjustment threshold value, the CO is adjusted by controlling the opening and closing frequency of the two-position two-way electromagnetic valve 2 The amount of air and air intake is up to CO 2 The concentration of the CO reaches the preparation requirement, and the adjustment threshold is adjusted according to the CO prepared by the requirement 2 Concentration ratio setting.
Compared with the prior art, the invention has the following advantages:
according to different use requirements of each culture cabin, the invention can keep the culture or sterilization state, and meanwhile, other culture cabins are not influenced, thereby perfectly solving the difficult problem of culture and sterilization selectivity and greatly improving the culture efficiency.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings may be obtained according to the drawings without inventive effort to a person skilled in the art.
FIG. 1 is a schematic diagram of a gas circuit control system for a honeycomb carbon dioxide incubator of the present invention.
FIG. 2 is CO 2 A preparation flow chart.
In the figure: 1. CO 2 A gas cylinder; 2. an air pump; 3. an air intake duct; 4. CO 2 A filter valve; 5. an air filter valve; 6. a primary filter; 7. a two-position two-way electromagnetic valve A; 8. a gas flow rate adjustment gauge; 9. a gas storage tank; 10. h 2 O 2 A busbar; 11. a piezoelectric pump A; 12. a secondary filter; 13. a culture cabin; 14. a third stage filter; 15. a two-position two-way solenoid valve B; 16. an exhaust electromagnetic valve; 17. h 2 O 2 A decomposer; 18. a pressure reducing valve; 19. CO 2 A concentration sensor; 20. CO 2 A busbar; 21. a two-position two-way solenoid valve C; 22. a digital flow switch; 23. a one-way valve, 24, a two-position three-way electromagnetic valve A; 25. a PTFE trachea; 26. an incubator body; 27. a piezoelectric pump B; 28. and a hydrogen peroxide generating device.
Detailed Description
It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other. The invention will be described in detail below with reference to the drawings in connection with embodiments. For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides a gas circuit system of a honeycomb carbon dioxide incubator, which mainly comprises: for preparing CO at required concentration 2 CO of gas 2 Gas preparation unit for producing vaporous H 2 O 2 Is in the vapor state H 2 O 2 The generating unit is used for controlling the air passage of the air inlet type according to the use requirement of each culture cabinThe incubator comprises a switching unit, an incubator body unit for performing culture tests and an exhaust unit for exhausting redundant gas in the incubator body unit.
Specifically, CO 2 The gas preparation unit mainly comprises CO 2 Gas cylinder, air pump, gas storage tank, CO 2 The air cylinder and the air pump are respectively connected with the air storage tank through one air inlet pipeline, and two-position two-way electromagnetic valves are arranged on the air inlet pipeline. Vaporous H 2 O 2 The generating unit comprises a hydrogen peroxide generating device and a piezoelectric pump, and is in a vapor state H 2 O 2 The generating unit is connected with the air inlet of the incubator main body unit. The first air inlet of the air circuit switching unit is connected with the CO 2 The gas outlet of the gas preparation unit is communicated with the gas outlet of the incubator main body unit through a first gas path pipeline, the second gas inlet is communicated with the gas outlet of the incubator main body unit through a second gas path pipeline, the gas outlet of the gas path switching unit is communicated with the gas inlet of the incubator main body unit, the gas outlet of the incubator is communicated with the gas exhaust unit through a gas exhaust pipeline, and a two-position two-way electromagnetic valve is arranged on the gas exhaust pipeline. The incubator body unit includes a plurality of culture tanks for performing independent experiments. The exhaust unit is an exhaust electromagnetic valve.
When the culture cabin works, the CO is controlled by the gas circuit switching unit 2 Gas is produced from the CO 2 The gas preparation unit enters the culture cabin and simultaneously blocks the vaporous state H 2 O 2 Entering; when the sterilizing is carried out, the steam state H is discharged by the piezoelectric pump 2 O 2 From the vapour state H 2 O 2 The generating unit is led into the main body unit of the incubator, and then the gas state H is controlled by the gas circuit switching unit 2 O 2 Enter the culture cabin and simultaneously block CO 2 And after the gas enters and reaches the disinfection time requirement, opening the exhaust electromagnetic valve.
As shown in FIG. 1, which is a preferred embodiment of the system of the present invention, the CO of the gas circuit system of the honeycomb carbon dioxide incubator of the present embodiment 2 The gas preparation unit mainly comprises CO 2 Gas cylinder 1, air pump 2, air inlet pipe 3 and CO 2 Filter valve 4, air filter 5, first-stage filter 6, two-position two-way electromagnetic valve A7, gas flow regulating meter 8 and storageA gas tank 9. Wherein the air path sequentially comprises an air pump 2, an air inlet pipeline 3, an air filter 5, a primary filter 6, a two-position two-way electromagnetic valve A and a gas flow regulating meter 8, and CO 2 The gas path sequentially comprises CO 2 The device comprises a gas cylinder 1, a gas inlet pipeline 3, an air filter 5, a primary filter 6, a two-position two-way electromagnetic valve A7, a gas flow regulating meter 8 and a pressure reducing valve 18. And the gas storage tank 9 is provided with CO 2 A concentration sensor 19.
Air outlet of air storage tank and CO 2 The busbar 20 is in communication with the first gas line; the air outlet of the incubator body unit 26 passes through H 2 O 2 The busbar 10 is in communication with a second gas path conduit.
The gas circuit switching unit comprises a plurality of two-position three-way electromagnetic valves 24 which are arranged in parallel, and a first gas inlet and CO of each two-position three-way electromagnetic valve 24 2 One branch of the output of the busbar 20 communicates with the second inlet and H 2 O 2 One branch of the output of the busbar 10 is communicated, and the air outlet of each two-position three-way electromagnetic valve 24 is communicated with one culture cabin 13. Further, CO 2 Each branch of the output of the busbar 20 is provided with a two-position two-way solenoid valve C21, a digital flow switch 22 and a one-way valve 23. A two-stage filter is arranged on the communication pipeline between the air outlet of the two-position three-way electromagnetic valve 24 and the culture cabin. Preferably, the communication pipeline between the air outlet of the two-position three-way electromagnetic valve 24 and the culture cabin is a PTFE air pipe. CO 2 A two-position two-way electromagnetic valve is arranged between one branch of the busbar output and the corresponding two-position three-way electromagnetic valve.
The incubator body 26 includes a plurality of culture chambers inside, each culture chamber 13 has an independent air inlet and air outlet, and the air outlet of the culture chamber 13 is provided with a three-stage filter 14 and a two-position two-way electromagnetic valve B15 on a communicating pipeline of the air outlet and the air discharging unit. Further, the exhaust unit further includes an H connected after the exhaust solenoid valve 16 2 O 2 A decomposer 17.
The invention uses air pump and CO 2 The gas cylinder is used as a gas source and passes through CO on the gas storage tank 2 Real-time concentration value fed back by concentration sensor, controlling on-off frequency of battery valve in air inlet loop and mixingSynthesizing the CO with the required concentration 2 And (3) mixing the gases. When the culture cabin works, CO 2 The electromagnetic valve of the corresponding air inlet passage is connected after the busbar, and the two-position three-way valve is CO 2 Gas entry, vapour H 2 O 2 In the blocking state, the gas flows into the culture cabin through secondary filtration, and the flow rate adjustment meter can control the flow rate of the gas correspondingly according to different numbers of culture cabins; when it is needed H 2 O 2 In the case of killing, first pass through H 2 O 2 The generator will be liquid H 2 O 2 Conversion to the vapour state H 2 O 2 And then the piezoelectric pump is used for providing power to meet the flowing condition. At this time, the air inlet solenoid valve of the corresponding culture cabin is closed, and H 2 O 2 The two-position three-way electromagnetic valve after the busbar is changed into CO 2 Gas blocking, vaporous H 2 O 2 When passing state and reaching the disinfection time requirement, the total exhaust battery valve is opened, and the steam state H 2 O 2 Enter H 2 O 2 In the decomposer, water and oxygen are decomposed. The system can finish CO by opening or closing the corresponding electromagnetic valve according to the same working state of the culture cabin 2 Gas supply and H 2 O 2 And (5) switching sterilization.
The invention also discloses a gas path control method of the honeycomb carbon dioxide incubator, which is realized based on the gas path system and comprises the following steps:
when the culture cabin works, CO 2 The electromagnetic valve of the corresponding air inlet passage is connected after the busbar, and the two-position three-way valve is CO 2 Gas entering, vapour state H 2 O 2 In the blocking state, the gas flows into the corresponding culture cabins, and meanwhile, the flow rate adjusting meter controls the flow rate of the gas correspondingly according to different numbers of culture cabins.
When H is 2 O 2 In the case of killing, first pass through H 2 O 2 The generator will be liquid H 2 O 2 Conversion to the vapour state H 2 O 2 Then the piezoelectric pump is used for providing power, at the moment, the air inlet electromagnetic valve of the corresponding culture cabin is closed, and H 2 O 2 The two-position three-way electromagnetic valve behind the busbar is CO 2 Gas blocking, vaporous H 2 O 2 When passing state and reaching the disinfection time requirement, the total exhaust battery valve is opened, and the steam state H 2 O 2 Enter H 2 O 2 Decomposing in a decomposer.
Further, when the culture cabin works, the method also comprises the step of configuring the CO with the required concentration 2 Specifically comprises the following steps:
obtaining CO requiring preparation 2 Concentration;
turning on CO 2 Two-position two-way electromagnetic valves arranged on the air inlet pipeline and the air inlet pipeline, and simultaneously inputting CO into the air storage tank 2 Gas and air, and through CO 2 Concentration sensor feedback CO 2 Concentration variation of (2);
when CO 2 When the concentration of (2) reaches the adjustment threshold value, the CO is adjusted by controlling the opening and closing frequency of the two-position two-way electromagnetic valve 2 The amount of air and air intake is up to CO 2 The concentration of the CO reaches the preparation requirement, and the adjustment threshold is adjusted according to the CO prepared by the requirement 2 Concentration ratio setting.
The method is further illustrated by the following examples.
To be filled with 5% concentration CO 2 For example, the air pump and CO are first turned on 2 The gas cylinder makes two gases enter the respective pipelines and according to the CO in the gas storage tank 2 The signal fed back by the concentration sensor controls the on-off of the electromagnetic valve, and maintains the entering amount of the two gases at different frequencies until the CO 2 The concentration value was 5%. Specific control frequency: when CO 2 If the concentration is lower than the specified concentration, closing the electromagnetic valve for air to enter and switching on CO 2 Solenoid valve, when CO 2 When the concentration reaches more than 4.5%, the electromagnetic valve frequency is changed to be on for 1S, off for 5S until the gas concentration is changed to be 5%, and the CO is closed 2 An electromagnetic valve; when CO 2 Above a specified concentration, turn off CO 2 The inlet electromagnetic valve is connected with the air inlet electromagnetic valve, when CO 2 When the concentration reaches within 5.5%, the solenoid valve frequency becomes on 1S, 5S off until the gas concentration becomes 5%, and the air solenoid valve is closed. Then the CO is pumped by an air pump of the flow regulating meter 2 Gas introduction designation cultureIn the cabin
Then the gas flows into the busbar, and when the incubator starts to cultivate, the corresponding electromagnetic valve is opened. When culture is needed, the two-position three-way electromagnetic valve is positioned at CO 2 The mixed gas is allowed to enter the state, the mixed gas smoothly flows into the incubator, and the electromagnetic valve for exhausting is in a closed state.
When the hydrogen peroxide sterilizing is started, when the hydrogen peroxide is required to be filled for sterilization, liquid hydrogen peroxide is firstly input into the hydrogen peroxide generator to vaporize the hydrogen peroxide, a PTFE hydrogen peroxide corrosion-resistant air pipe is used, and then gaseous hydrogen peroxide is introduced into the specified culture cabin through an on-off electromagnetic valve. At this time, the two-position three-way electromagnetic valve is connected, CO 2 The mixed gas channel is closed, the vaporous hydrogen peroxide channel is opened, the electromagnetic valve for exhausting is opened, and the piezoelectric pump starts to work, so that the vaporous hydrogen peroxide enters the culture cabin. And controlling the supply amount of the vaporous hydrogen peroxide by a pressure sensor. When the disinfection time is reached, a two-position three-way electromagnetic valve of the main exhaust port is connected, so that the vaporous hydrogen peroxide enters the hydrogen peroxide decomposition device.
The CO of different cabins can be switched at any time by controlling the on-off states of the two-position two-way electromagnetic valve and the two-position three-way electromagnetic valve at different positions in the gas circuit 2 Concentration control or hydrogen peroxide sterilization.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (6)
1. A gas circuit system of a honeycomb carbon dioxide incubator, comprising:
CO 2 a gas preparation unit, the CO 2 The gas preparation unit mainly comprises CO 2 Gas cylinder and air pumpA gas storage tank, the CO 2 The gas cylinder and the air pump are respectively connected with the gas storage tank through a gas inlet pipeline, a two-position two-way electromagnetic valve is arranged on the gas inlet pipeline, and the CO 2 The gas preparation unit is connected with CO 2 The busbar is communicated with the first air passage pipeline;
vaporous H 2 O 2 A generation unit for generating the steam state H 2 O 2 The generating unit comprises a hydrogen peroxide generating device and a piezoelectric pump, and the steam state H is 2 O 2 The generating unit is connected with the air inlet of the incubator main body unit, and the air outlet of the incubator main body unit is connected with the air inlet of the incubator main body unit through H 2 O 2 The busbar is communicated with the second gas path pipeline;
the gas circuit switching unit, the first air inlet of the gas circuit switching unit and the CO 2 The gas outlet of the gas preparation unit is communicated with the gas outlet of the incubator main body unit through a first gas path pipeline, the second gas inlet is communicated with the gas outlet of the incubator main body unit through a second gas path pipeline, the gas outlet of the gas path switching unit is communicated with the air inlet of the incubator main body unit, the gas outlet of the incubator is communicated with the gas exhaust unit through a gas exhaust pipeline, a two-position two-way electromagnetic valve is arranged on the gas exhaust pipeline, the gas path switching unit comprises a plurality of two-position three-way electromagnetic valves which are arranged in parallel, and the first gas inlet of each two-position three-way electromagnetic valve is communicated with CO 2 One branch of the busbar output is communicated with the second air inlet and H 2 O 2 The gas outlet of each two-position three-way electromagnetic valve is communicated with a culture cabin;
the incubator comprises an incubator main body unit, a plurality of incubator control units and a control unit, wherein the incubator main body unit comprises a plurality of incubator cabins;
the exhaust unit is an exhaust electromagnetic valve; when the culture cabin works, the CO is controlled by the gas circuit switching unit 2 Gas is produced from the CO 2 The gas preparation unit enters the culture cabin and simultaneously blocks the vaporous state H 2 O 2 Entering; when the sterilizing is carried out, the steam state H is discharged by the piezoelectric pump 2 O 2 From the vapour state H 2 O 2 The generating unit is led into the main body unit of the incubator, and then the gas state H is controlled by the gas circuit switching unit 2 O 2 Enter into cultureCabin, at the same time block CO 2 And after the gas enters and reaches the disinfection time requirement, opening the exhaust electromagnetic valve.
2. The gas circuit system of a honeycomb carbon dioxide incubator of claim 1, wherein the exhaust unit further comprises an H connected after an exhaust solenoid valve 2 O 2 A decomposer.
3. The gas circuit system of a honeycomb carbon dioxide incubator of claim 1, wherein the CO 2 A two-position two-way electromagnetic valve is arranged between one branch of the busbar output and the corresponding two-position three-way electromagnetic valve.
4. The gas circuit system of a honeycomb carbon dioxide incubator of claim 1, wherein CO 2 The air inlet pipeline for connecting and communicating the air cylinder with the air storage tank is also provided with CO 2 A filter valve, a filter, and a gas flow rate adjustment meter;
an air filter valve, a filter and a gas flow regulating meter are also arranged on an air inlet pipeline connected and communicated with the air pump and the air storage tank.
5. A method for controlling the air path of a honeycomb carbon dioxide incubator, which is realized based on the air path system of any one of claims 1 to 4, and is characterized by comprising the following steps:
when the culture cabin works, CO 2 The electromagnetic valve of the corresponding air inlet passage is connected after the busbar, and the two-position three-way valve is CO 2 Gas entering, vapour state H 2 O 2 In a blocking state, gas flows into the corresponding culture cabins, and meanwhile, the flow rate adjusting meter controls the flow rate of the gas correspondingly according to different numbers of culture cabins;
when H is 2 O 2 In the case of killing, first pass through H 2 O 2 The generator will be liquid H 2 O 2 Conversion to the vapour state H 2 O 2 The power is provided by the piezoelectric pump, and the air inlet electromagnetic of the corresponding culture cabin is provided at the momentThe valve is closed and H 2 O 2 The two-position three-way electromagnetic valve behind the busbar is CO 2 Gas blocking, vaporous H 2 O 2 When passing state, after reaching the disinfection time requirement, the total exhaust electromagnetic valve is opened, and the steam state H 2 O 2 Enter H 2 O 2 Decomposing in a decomposer.
6. The method of claim 5, further comprising configuring the desired concentration of CO when the incubator is in operation 2 Specifically comprises the following steps:
obtaining CO requiring preparation 2 Concentration;
turning on CO 2 Two-position two-way electromagnetic valves arranged on the air inlet pipeline and the air inlet pipeline, and simultaneously inputting CO into the air storage tank 2 Gas and air, and through CO 2 Concentration sensor feedback CO 2 Concentration variation of (2);
when CO 2 When the concentration of (2) reaches the adjustment threshold value, the CO is adjusted by controlling the opening and closing frequency of the two-position two-way electromagnetic valve 2 The amount of air and air intake is up to CO 2 The concentration of the CO reaches the preparation requirement, and the adjustment threshold is adjusted according to the CO prepared by the requirement 2 Concentration ratio setting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111460176.1A CN114106990B (en) | 2021-12-02 | 2021-12-02 | Air path system and air path control method of honeycomb carbon dioxide incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111460176.1A CN114106990B (en) | 2021-12-02 | 2021-12-02 | Air path system and air path control method of honeycomb carbon dioxide incubator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114106990A CN114106990A (en) | 2022-03-01 |
| CN114106990B true CN114106990B (en) | 2023-08-29 |
Family
ID=80366325
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111460176.1A Active CN114106990B (en) | 2021-12-02 | 2021-12-02 | Air path system and air path control method of honeycomb carbon dioxide incubator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114106990B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010154793A (en) * | 2008-12-26 | 2010-07-15 | Sanyo Electric Co Ltd | Co2 incubator |
| CN108641953A (en) * | 2018-05-16 | 2018-10-12 | 余春 | Totally enclosed type intelligent biology production system and production method |
| CN110540937A (en) * | 2019-09-09 | 2019-12-06 | 英诺维尔智能科技（苏州）有限公司 | Multi-compartment unit culture system suitable for GMP (good manufacturing practice) compliance automatic production |
| CN111154638A (en) * | 2020-03-09 | 2020-05-15 | 广西贵港华堂天诺微生物科技有限公司 | Microbial cultivation case is used in biotechnology laboratory |
| WO2021220784A1 (en) * | 2020-04-30 | 2021-11-04 | 株式会社エアレックス | Incubator |
| CN113621507A (en) * | 2021-07-12 | 2021-11-09 | 冰山松洋生物科技(大连)有限公司 | Full-automatic carrying and independent temperature and CO control2Multi-culture-cabin incubator with concentration |
-
2021
- 2021-12-02 CN CN202111460176.1A patent/CN114106990B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010154793A (en) * | 2008-12-26 | 2010-07-15 | Sanyo Electric Co Ltd | Co2 incubator |
| CN108641953A (en) * | 2018-05-16 | 2018-10-12 | 余春 | Totally enclosed type intelligent biology production system and production method |
| CN110540937A (en) * | 2019-09-09 | 2019-12-06 | 英诺维尔智能科技（苏州）有限公司 | Multi-compartment unit culture system suitable for GMP (good manufacturing practice) compliance automatic production |
| CN111154638A (en) * | 2020-03-09 | 2020-05-15 | 广西贵港华堂天诺微生物科技有限公司 | Microbial cultivation case is used in biotechnology laboratory |
| WO2021220784A1 (en) * | 2020-04-30 | 2021-11-04 | 株式会社エアレックス | Incubator |
| CN113621507A (en) * | 2021-07-12 | 2021-11-09 | 冰山松洋生物科技(大连)有限公司 | Full-automatic carrying and independent temperature and CO control2Multi-culture-cabin incubator with concentration |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114106990A (en) | 2022-03-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103910426B (en) | Drinking water disinfecting and purifying plant used for centralized water supply in residential area | |
| US20110027146A1 (en) | Sterilizer | |
| CN113046225A (en) | Gas supply system for cell culture | |
| US20030173309A1 (en) | Process for producing ozone-containing sterilizing water and an apparatus used therefor | |
| CN114106990B (en) | Air path system and air path control method of honeycomb carbon dioxide incubator | |
| CN204569507U (en) | Vehicular ozone water apparatus | |
| GB2607754A (en) | Process and apparatus for production of ozone | |
| CN115386488A (en) | Gas supply system for cell culture box and gas concentration dynamic control method | |
| CN110479123A (en) | Intelligent automatic hydrogen nitrogen matching device | |
| CN115627214A (en) | Three-gas multi-channel gas supply system and method for incubator | |
| CN101868113A (en) | Gas injection system and the method that is used to move gas injection system | |
| RU2596396C1 (en) | Bioreactor with membrane device for gas supply of microorganisms | |
| CN217909767U (en) | Deodorization system | |
| CN215161801U (en) | Hydroxyl radical aqueous solution and drinking water supply system | |
| EP1309515B1 (en) | A process for producing ozone-containing sterilizing water and an apparatus used therefor | |
| CN220132234U (en) | Fermentation culture system of uninterrupted air feed | |
| CN209513550U (en) | A kind of skid-mounted type leak source corrosion experimental device | |
| CN209981180U (en) | Gas automatic control energy-saving system and semiconductor processing equipment | |
| CN208082247U (en) | Ozone water disinfection system with dehydrogenation unit | |
| CN201647989U (en) | Chlorine dioxide generating device | |
| CN221176278U (en) | Integrated application device for producing oxygen and fuel cell by using integrated electrolyzed water | |
| AU2001280225A1 (en) | A process for producing ozone-containing sterilizing water and an apparatus used therefor | |
| TW201217584A (en) | comprising a hydrogen/oxygen dissociator, an ozone generator and a water gas separator | |
| CN110436675A (en) | A kind of oxygen-enriched water preparation apparatus and preparation method | |
| CN217103408U (en) | Disinfection and water purification integrated equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |