CN113046225A - Gas supply system for cell culture - Google Patents

Gas supply system for cell culture Download PDF

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Publication number
CN113046225A
CN113046225A CN202110287584.5A CN202110287584A CN113046225A CN 113046225 A CN113046225 A CN 113046225A CN 202110287584 A CN202110287584 A CN 202110287584A CN 113046225 A CN113046225 A CN 113046225A
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gas
mixing
chamber
air
culture
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Inventor
曾维俊
肖冬根
孙海旋
宋宇
赵振英
于福鑫
刘欣瑞
杨森
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Jinan Guoke Medical Engineering Technology Development Co ltd
Suzhou Institute of Biomedical Engineering and Technology of CAS
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Jinan Guoke Medical Engineering Technology Development Co ltd
Suzhou Institute of Biomedical Engineering and Technology of CAS
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Priority to CN202110287584.5A priority Critical patent/CN113046225A/en
Publication of CN113046225A publication Critical patent/CN113046225A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

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  • Health & Medical Sciences (AREA)
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Abstract

The invention discloses a gas supply system for cell culture, which comprises: the device comprises a gas source module, a gas mixing module and a culture chamber gas supply module; the gas mixing module comprises a mass flow controller, a mixing cavity, a concentration sensor and a central controller; cultivate room air feed module include with a plurality of air feed units of a plurality of cultivation room one-to-ones, every air feed unit all include with correspond the air feed pump that the inlet end of cultivation room is connected and with correspond cultivate the check valve that the room's the end of giving vent to anger is connected. The gas supply system for cell culture can realize the on-line dynamic mixing and the rapid concentration adjustment of three gases, and has high gas concentration control stability; the invention can reduce the gas consumption of the gas supply system by recycling the gas exhausted from the culture chamber; the invention can support a plurality of culture chambers to supply air simultaneously and independently, and can not influence the normal work of the air circuit of the system and other culture chambers when the operation of part of the culture chambers is carried out.

Description

Gas supply system for cell culture
Technical Field
The invention relates to the field of cell culture, in particular to a gas supply system for cell culture.
Background
The mixed gas for cell culture is one of the necessary conditions for cell culture and survival in vitro, and the main components of the mixed gas are oxygen, carbon dioxide and nitrogen. Oxygen can support cellular respiration, produce energy for cell growth and synthesize various components required for cell growth. Carbon dioxide is both a metabolite of the cells and a component required for growth and propagation of the cells, and its main role in cell culture is to adjust and maintain the pH of the culture solution, and most cells have a suitable pH of 7.2-7.4, and deviation from this range will have a detrimental effect on cell culture. Taking the in vitro culture of embryo cells as an example, in order to ensure the optimal development state of the embryo, the mixture ratio of the main stream is 5% CO2、5％O2And 90% N2。
At present, two gas supply modes of a cell culture box exist, wherein one mode is to supply CO2Supplying CO as gas source into the incubator2Controlling the CO in the initial air in the tank2The proportion, typically the final mixed gas, is 5% CO2And 95% air, thus O2And N2The relative proportions of (A) and (B) are fixed, and the method is suitable for conventional cell culture; another is to convert CO2、N2And air (or O)2) As gas source, supplying mixed gas to the incubator for CO2And O2The concentration of the compound can be controlled and adjusted, the strict requirements of In Vitro Fertilization (IVF) embryonic cells, stem cell culture and the like can be met, and the application range is wider. The air supply mode of the existing cell culture box generally only supports the air supply of a single large chamber, all cells are cultured in the same space, any culture vessel is taken and placed, the box opening operation is needed, the temperature and the gas environment of the culture chamber are easily damaged, new balance conditions need to be reestablished, potential damage can be caused to the cells, and meanwhile, the air consumption can be increased.
Disclosure of Invention
The present invention aims to solve the above-mentioned problems of the prior art and provide a gas supply system for cell culture.
In order to solve the technical problems, the invention adopts the technical scheme that: a gas supply system for cell culture, comprising: the device comprises a gas source module, a gas mixing module and a culture chamber gas supply module;
the gas mixing module uniformly mixes a plurality of gases provided by the gas source module and then conveys the mixed gases to the culture room gas supply module, the gas mixing module comprises a mass flow controller, a mixing cavity, a concentration sensor and a central controller, each gas is respectively conveyed into the mixing cavity for mixing after controlling the gas inflow through an independent mass flow controller, and the central controller calculates the demand of each gas according to the difference value between the monitoring value of the concentration sensor in the mixing cavity and a preset target value and controls the mass flow controller so as to adjust the gas inflow of each gas;
the culture chamber air supply module comprises a plurality of air supply units which are in one-to-one correspondence with a plurality of culture chambers, each air supply unit comprises an air supply pump connected with the air inlet end of the corresponding culture chamber and a one-way valve connected with the air outlet end of the corresponding culture chamber, the air supply pump is connected with the air supply port of the mixing cavity, and the one-way valve is connected with the air return port of the mixing cavity; the mixed gas in the mixing chamber is discharged from the gas supply port and then enters the culture chamber under the action of the gas supply pump, and the mixed gas flows through the culture chamber and then sequentially enters the check valve and the gas return port to return to the mixing chamber to form a circulating gas path.
Preferably, the gas source module is used for providing a plurality of gases, and includes a plurality of compressed gas source units, each compressed gas source unit includes a compressed gas cylinder, a pressure reducing valve connected to a gas outlet of the compressed gas cylinder, and a filter connected to the pressure reducing valve, and the gas in the compressed gas cylinder is decompressed by the pressure reducing valve and filtered by the filter, and then is input into the mixing chamber.
Preferably, the air source module further comprises an air source unit, the air source unit comprises an air inlet pump and a filter connected with the air inlet pump, and air is filtered by the filter and then input into the mixing cavity under the action of the air inlet pump.
Preferably, the mixing chamber is communicated with the atmosphere through a pressure relief valve, the pressure relief valve is a one-way valve, and when the pressure in the mixing chamber is higher than the atmospheric pressure, the gas in the mixing chamber can be discharged to the atmosphere through the pressure relief valve.
Preferably, the baffle through the crisscross setting of a plurality of intervals in the mixing chamber forms a plurality of sub-chambeies, and a plurality of sub-chambeies form snakelike gas mixing passage end to end in proper order.
Preferably, a plurality of air inlets are arranged on the side wall of the first sub-cavity at the air inlet side of the mixing cavity.
Preferably, a pressure relief opening is formed in the side wall of the last sub-cavity on the air inlet side of the mixing cavity, and the pressure relief valve is arranged on the pressure relief opening.
Preferably, the return air port is communicated with the first sub-chamber, the supply air port is communicated with the last sub-chamber, and the concentration sensor is arranged in the last sub-chamber.
Preferably, the concentration sensor comprises CO2Concentration sensor, O2Concentration sensor, N2One or more of the concentration sensors.
The invention has the beneficial effects that:
the gas supply system for cell culture can realize the on-line dynamic mixing and the rapid concentration adjustment of three gases, and has high gas concentration control stability; the invention can reduce the gas consumption of the gas supply system by recycling the gas exhausted from the culture chamber; the invention can support a plurality of culture chambers to supply air simultaneously and independently, and can not influence the normal work of the air circuit of the system and other culture chambers when the operation of part of the culture chambers is carried out.
Drawings
FIG. 1 is a schematic view showing the construction of a gas supply system for cell culture according to the present invention;
fig. 2 is a schematic structural view of a mixing chamber of the present invention.
Description of reference numerals:
1-gas source module; 10-compressed air source unit; 11-a compressed gas cylinder; 12-a pressure reducing valve; 13-a filter; 14-an air supply unit; 15-an air intake pump; 16-a filter;
2-a gas mixing module; 20-mass flow controller; 21-a mixing chamber; 22-concentration sensor; 23-a central controller; 24-a pressure relief valve; 210-a separator; 211 — the first subchamber; 212 — last subchamber; 213-air inlet; 214 — a pressure relief port; 215-air supply port; 216 — air return port;
3-culture room air supply module; 30-an air supply unit; 31-air supply pump; 32-a one-way valve; 33-culture chamber.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
As shown in fig. 1-2, a gas supply system for cell culture according to the present embodiment includes: the device comprises a gas source module 1, a gas mixing module 2 and a culture chamber gas supply module 3;
the gas mixing module 2 uniformly mixes a plurality of gases provided by the gas source module 1 and then conveys the mixed gases to the culture room gas supply module 3, the gas mixing module 2 comprises a mass flow controller 20, a mixing cavity 21, a concentration sensor 22 and a central controller 23, each gas is respectively conveyed into the mixing cavity 21 to be mixed after controlling the gas inflow through an independent mass flow controller 20, the central controller 23 calculates the required quantity of each gas according to the difference value between the monitoring value of the concentration sensor 22 in the mixing cavity 21 and a preset target value, and controls the mass flow controller 20 to adjust the gas inflow of each gas;
the culture chamber air supply module 3 comprises a plurality of air supply units 30 corresponding to the plurality of culture chambers 33 one by one, each air supply unit 30 comprises an air supply pump 31 connected with the air inlet end of the corresponding culture chamber 33 and a one-way valve 32 connected with the air outlet end of the corresponding culture chamber 33, the air supply pump 31 is connected with an air supply port 215 of the mixing cavity 21, and the one-way valve 32 is connected with an air return port 216 of the mixing cavity 21; the mixed gas in the mixing chamber 21 is discharged from the gas supply port 215 and then enters the culture chamber 33 under the action of the gas supply pump 31, and the mixed gas flows through the culture chamber 33 and then sequentially enters the check valve 32 and the gas return port 216 to return to the mixing chamber 21, so that a circulating gas path is formed.
The gas source module 1 is used for providing a plurality of gases, and includes a plurality of compressed gas source units 10, each compressed gas source unit 10 includes a compressed gas cylinder 11, a pressure reducing valve 12 connected to a gas outlet of the compressed gas cylinder 11, and a filter 13 connected to the pressure reducing valve 12, and the gas in the compressed gas cylinder 11 is decompressed by the pressure reducing valve 12, filtered by the filter 13, and then is input into the mixing chamber 21.
The gas source module 1 has different gas source types according to the concentration requirement of the required mixed gas, when low oxygen (O) is required2Concentration below 20%) can be made of pure CO2Pure N2And air when high oxygen (O) is required2Concentration of more than 20%) environment, pure CO is required2Pure N2And pure O2And (4) forming. In order to fully express the more general technical scheme, the embodiment is described by taking the scheme of the gas supply system of the mixed gas in the low-oxygen culture environment as an example.
In this embodiment, the compressed air source unit 10 includes 2 compressed air source unitsFor supplying pure CO respectively2And pure N2. Further, the air supply module 1 further includes an air supply unit 14, the air supply unit 14 includes an intake pump 15 and a filter 16 connected to the intake pump 15, and the air is filtered by the filter 16 under the action of the intake pump 15 and then is input into the mixing chamber 21. Pure CO2Pure N2And air pressure at the end may be brought to a suitable range after pressure reduction or pumping treatment, ready for entry into the mixing chamber 21.
Pure CO2Pure N2And air respectively pass through respective mass flow controllers 20 and then are introduced into a mixing chamber 21, a concentration sensor 22 is arranged in the mixing chamber 21 and can comprise CO2Concentration sensor, O2Concentration sensor, N2One or more of the concentration sensors, in this embodiment comprising CO2And O2The central controller 23 is in data signal communication with the mass flow controllers 20 and the concentration sensors 22, and the central controller 23 calculates the required amount of each gas according to the difference between the monitoring value of the concentration sensor 22 in the mixing cavity 21 and a preset target value, and then sends an instruction to the corresponding mass flow controller 20 to control the volume of different gases entering the mixing cavity 21, so that the gas components of the mixed gas are dynamically adjusted in real time.
In a preferred embodiment, the mixing chamber 21 is connected to the atmosphere through a pressure relief valve 24, and the pressure relief valve 24 is a one-way valve, so that when the pressure in the mixing chamber 21 is higher than the atmospheric pressure, the gas in the mixing chamber 21 will be discharged to the atmosphere through the pressure relief valve 24. Through the setting of relief valve 24, when the gas mixture concentration dynamic adjustment, can in time discharge unnecessary gas, be favorable to the quick realization of concentration ratio, can also make the internal pressure of whole cultivation gas circuit keep at the level equal with atmospheric pressure in addition, match with concentration sensor 22's service pressure, make the monitoring more accurate, also can not cause high pressure environment to the cell and influence cell development simultaneously.
In a preferred embodiment, the mixing chamber 21 is a closed chamber, a plurality of necessary air vents are reserved, and a plurality of sub-chambers are formed in the mixing chamber 21 by a plurality of partition plates 210 arranged alternately at intervalsThe chamber, a plurality of sub-chamber end to end in proper order form snakelike air mixture way, can promote the intensive mixing of gas mixture in the intracavity. Wherein, the side wall of the first sub-cavity 211 at the air inlet side of the mixing cavity 21 is provided with three air inlets 213 for pure CO2Pure N2And air. A pressure relief port 214 is provided in the side wall of the last subchamber 212 on the intake side of mixing chamber 21, and a pressure relief valve 24 is provided in pressure relief port 214.
Further, wherein, return air port 216 and first sub-chamber 211 intercommunication, the gaseous mixing chamber 21 that gets into through return air port 216 that cultivates the room 33 and return meets with new make-up gas to through snakelike gas mixing path intensive mixing, thereby realize the make full use of gas circuit circulation and gas mixture, reduce the gas loss volume.
Wherein, gas supply port 215 communicates with last subchamber 212, and concentration sensor 22 sets up in last subchamber 212 to monitor the concentration of the gas after mixing, if the concentration has the deviation, central controller 23 will be according to the deviation volume, replenish different kinds of gas in mixing chamber 21.
The culture chamber air supply module 3 comprises a plurality of culture chambers 33, wherein the front end of each culture chamber 33 is connected with an air supply pump 31, the rear end is connected with a one-way valve 32, the air supply pump 31 is communicated with an air supply port 215 of the mixing cavity 21, and the one-way valve 32 is communicated with an air return port 216 of the mixing cavity 21. The mixed gas outputted from the mixing chamber 21 is introduced into each of the culture chambers 33 by the air supply pump 31, passes through the check valve 32, and is returned to the mixing chamber 21, thereby forming a closed-cycle gas path system. The gas supply pump 31 is preferably a positive displacement pump, such as a diaphragm pump, which can be shut off when not in use, and the one-way valve 32 allows the growth chamber 33 gas to pass to the mixing chamber 21, and vice versa. When a user opens a certain culture chamber 33, the air supply pump 31 is closed after the cover opening sensor detects a cover opening signal, the front end air passage of the culture chamber 33 is blocked, because the pressure of the system air passage is slightly higher than the atmospheric pressure, under the action of the one-way valve 32, the internal air is prevented from being discharged to the atmosphere, the rear air passage of the culture chamber 33 can be blocked, and the normal work of the system air passage and other culture chambers is not influenced when the operation of the certain culture chamber is carried out.
While embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields of application of the invention, and further modifications may readily be effected by those skilled in the art, so that the invention is not limited to the specific details without departing from the general concept defined by the claims and the scope of equivalents.

Claims (9)

1. A gas supply system for cell culture, comprising: the device comprises a gas source module, a gas mixing module and a culture chamber gas supply module;
the gas mixing module uniformly mixes a plurality of gases provided by the gas source module and then conveys the mixed gases to the culture room gas supply module, the gas mixing module comprises a mass flow controller, a mixing cavity, a concentration sensor and a central controller, each gas is respectively conveyed into the mixing cavity for mixing after controlling the gas inflow through an independent mass flow controller, and the central controller calculates the demand of each gas according to the difference value between the monitoring value of the concentration sensor in the mixing cavity and a preset target value and controls the mass flow controller so as to adjust the gas inflow of each gas;
the culture chamber air supply module comprises a plurality of air supply units which are in one-to-one correspondence with a plurality of culture chambers, each air supply unit comprises an air supply pump connected with the air inlet end of the corresponding culture chamber and a one-way valve connected with the air outlet end of the corresponding culture chamber, the air supply pump is connected with the air supply port of the mixing cavity, and the one-way valve is connected with the air return port of the mixing cavity; the mixed gas in the mixing chamber is discharged from the gas supply port and then enters the culture chamber under the action of the gas supply pump, and the mixed gas flows through the culture chamber and then sequentially enters the check valve and the gas return port to return to the mixing chamber to form a circulating gas path.
2. The gas supply system for cell culture according to claim 1, wherein the gas source module is used for supplying a plurality of gases, and comprises a plurality of compressed gas source units, each compressed gas source unit comprises a compressed gas cylinder, a pressure reducing valve connected to a gas outlet of the compressed gas cylinder, and a filter connected to the pressure reducing valve, and the gases in the compressed gas cylinders are decompressed by the pressure reducing valves and filtered by the filters and then input into the mixing cavity.
3. The air supply system for cell culture according to claim 2, wherein the air supply module further comprises an air supply unit, the air supply unit comprises an air supply pump and a filter connected with the air supply pump, and air is filtered by the filter and then is input into the mixing cavity under the action of the air supply pump.
4. The gas supply system for cell culture according to claim 1, wherein the mixing chamber is connected to the atmosphere through a pressure release valve, the pressure release valve is a one-way valve, and when the pressure in the mixing chamber is higher than the atmospheric pressure, the gas in the mixing chamber is discharged to the atmosphere through the pressure release valve.
5. The gas supply system for cell culture according to claim 4, wherein a plurality of sub-cavities are formed in the mixing cavity by a plurality of partition plates which are arranged at intervals in a staggered mode, and the plurality of sub-cavities are sequentially connected end to form the snake-shaped mixing gas channel.
6. A gas supply system for cell culture as claimed in claim 5, wherein a plurality of gas inlets are provided on the side wall of the first sub-chamber on the gas inlet side of the mixing chamber.
7. The gas supply system for cell culture according to claim 6, wherein a pressure relief opening is provided on a side wall of the last sub-chamber on the gas inlet side of the mixing chamber, and the pressure relief valve is provided on the pressure relief opening.
8. A gas supply system for cell culture as claimed in claim 7, wherein the gas return port communicates with the first sub-chamber, the gas supply port communicates with the last sub-chamber, and the concentration sensor is disposed in the last sub-chamber.
9. A gas supply system for cell culture as claimed in claim 8, wherein the concentration sensor comprises CO2Concentration sensor, O2Concentration sensor, N2One or more of the concentration sensors.
CN202110287584.5A 2021-03-17 2021-03-17 Gas supply system for cell culture Pending CN113046225A (en)

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CN113862402A (en) * 2021-10-18 2021-12-31 冰山松洋生物科技(大连)有限公司 Gas concentration control method of three-gas incubator
CN114196538A (en) * 2021-12-02 2022-03-18 冰山松洋生物科技(大连)有限公司 Multi-culture-cabin CO2Concentration independent adjusting method and gas circuit system
CN115369189A (en) * 2022-09-02 2022-11-22 广州市华粤行医疗科技有限公司 Air path control method and system for multiple incubators
CN115558593A (en) * 2022-09-02 2023-01-03 广州市华粤行医疗科技有限公司 Medium culture observation device and air path control method thereof
CN116622918A (en) * 2023-07-20 2023-08-22 中国科学院苏州生物医学工程技术研究所 Air inlet control method for two-gas supply system of cell incubator

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CN108587906A (en) * 2018-06-15 2018-09-28 东南大学 A kind of high-precision quickly changes the cell culture apparatus of oxygen environment
CN211570629U (en) * 2019-11-25 2020-09-25 武汉互创联合科技有限公司 Mixing loop of two gases
CN212025366U (en) * 2019-12-24 2020-11-27 青海大学附属医院 Cell tissue culture device capable of accurately controlling gas pressure and concentration

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CN108384714A (en) * 2018-04-23 2018-08-10 苏州欧飞纳米科技有限公司 A kind of gas-dynamic control system of biological cell reactor
CN108587906A (en) * 2018-06-15 2018-09-28 东南大学 A kind of high-precision quickly changes the cell culture apparatus of oxygen environment
CN211570629U (en) * 2019-11-25 2020-09-25 武汉互创联合科技有限公司 Mixing loop of two gases
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113862402A (en) * 2021-10-18 2021-12-31 冰山松洋生物科技(大连)有限公司 Gas concentration control method of three-gas incubator
CN114196538A (en) * 2021-12-02 2022-03-18 冰山松洋生物科技(大连)有限公司 Multi-culture-cabin CO2Concentration independent adjusting method and gas circuit system
CN115369189A (en) * 2022-09-02 2022-11-22 广州市华粤行医疗科技有限公司 Air path control method and system for multiple incubators
CN115558593A (en) * 2022-09-02 2023-01-03 广州市华粤行医疗科技有限公司 Medium culture observation device and air path control method thereof
WO2024046258A1 (en) * 2022-09-02 2024-03-07 广州市华粤行医疗科技有限公司 Medium culture observation apparatus and gas path control method therefor
CN116622918A (en) * 2023-07-20 2023-08-22 中国科学院苏州生物医学工程技术研究所 Air inlet control method for two-gas supply system of cell incubator
CN116622918B (en) * 2023-07-20 2023-11-28 中国科学院苏州生物医学工程技术研究所 Air inlet control method for two-gas supply system of cell incubator

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