CN115627214A - Three-gas multi-channel gas supply system and method for incubator - Google Patents

Three-gas multi-channel gas supply system and method for incubator Download PDF

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CN115627214A
CN115627214A CN202211336491.8A CN202211336491A CN115627214A CN 115627214 A CN115627214 A CN 115627214A CN 202211336491 A CN202211336491 A CN 202211336491A CN 115627214 A CN115627214 A CN 115627214A
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gas
gas supply
chamber
air
culture
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肖冬根
钱俊
曾维俊
陈瑞涛
闫雪松
孙海旋
曹旭刚
赵振英
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Jinan Guoke Medical Engineering Technology Development Co ltd
Suzhou Institute of Biomedical Engineering and Technology of CAS
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Jinan Guoke Medical Engineering Technology Development Co ltd
Suzhou Institute of Biomedical Engineering and Technology of CAS
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract

The invention discloses a three-gas multi-channel gas supply system and a method for an incubator, wherein the system comprises: the gas source module comprises three gas source units for respectively and independently providing CO 2 、O 2 、N 2 Three pure gases; the gas mixing module comprises three mass flow controllers respectively connected with the three gas source units, a mixing cavity connected with the three mass flow controllers, and a pressure sensor for detecting gas pressure in the mixing cavity; the culture chamber gas supply module comprises a main gas supply pipeline connected with the mixing cavity and N gas supply pipelines arranged in parallel on the main gas supply pipelineAn air supply unit; and a gas detection module. The invention can realize the on-line dynamic mixing and the quick concentration adjustment of three gases, and has the advantages of quick response, stable and accurate concentration control and simple control; the culture room provided with the plurality of air supply channels is expandable, and the culture rooms at the tail ends of the plurality of air supply channels are independent from each other and do not influence each other during operation; the invention can ensure that the gas concentration in each culture chamber is in a required range.

Description

Three-gas multi-channel gas supply system and method for incubator
Technical Field
The invention relates to the field of cell culture, in particular to a three-gas multi-channel gas supply system and a three-gas multi-channel gas supply method for an incubator.
Background
The mixed gas is one of the necessary conditions for the in vitro culture and survival of living cells, and the main components of the mixed gas are oxygen, carbon dioxide and nitrogen. Oxygen can support cellular respiration, producing energy for cell growth and synthesizing various components for cell growth. Carbon dioxide is both a cell metabolite and a component required for cell growth and propagation, and the main function of carbon dioxide in cell culture is to adjust and maintain the pH value of the culture solution, and the proper pH value of most cells is 7.2-7.4, and deviation from the range has harmful effect on cell culture. In the case of in vitro culture of embryonic cells, to ensure the optimal development of the embryo, the ratio of the main gas mixture is 6% 2 、5％O 2 And 89% of N 2 。
The living cell culture is generally carried out in an incubator, and at present, the incubator has two gas supply modes, one is CO 2 Supplying CO as a single gas source to the incubator 2 Controlling the CO in the initial air in the tank 2 The proportion, typically the final mixed gas, is 5% CO 2 And 95% air, thus O 2 And N 2 The relative proportion of the components is fixed, and the cell culture medium is suitable for conventional cell culture and has good sealing performance; another is to convert CO 2 、N 2 And air (or O) 2 ) The mixed gas is used as a gas source and is supplied into the incubator, so that the strict requirements of In Vitro Fertilization (IVF) embryonic cells, stem cell culture, organoid culture and the like can be met, and the application range is wider.
The methods for supplying the mixed gas to the incubator are generally two types:
the first method is to mix the three gases in a predetermined ratio and compress them in a gas tank, and to supply the mixed gas directly from a single gas tank to the incubator. The method only needs a single mixed gas source, has simple structure, can directly purchase premixed gas, is more convenient to use, but has the problems that the proportion of the premixed gas is fixed and unchangeable, the gas is delaminated again in the storage and use processes, the gas supply concentration is unstable, and the like, and has certain limitation in use.
The second is that the three air sources are directly mixed according to the set proportion and supply air to the incubator. For example, patent CN113046225A discloses a solution for controlling CO separately using 3 mass flow controllers 2 、O 2 And N 2 The three gases enter the mixing cavity, are supplied to the culture chamber from the mixing cavity through the air pump and return to the mixing cavity for circulation. The method needs to provide three gases simultaneously, needs a blending and gas concentration regulation and control device, so that the system is more complex, but can regulate and change CO in real time 2 And O 2 Is suitable for various environments. However, this solution also has the following problems: (1) The air input of each culture chamber is controlled by an air pump, and the flow is inaccurate; (2) The method of gas circulation has high requirement on the sealing performance of the culture chamber; (3) When the cover of any culture room is opened, air is mixed, the influence on the air supply concentration of all culture rooms is large, and the independence among different channels cannot be realized; (4) The solution of discharging the original gas by introducing a new gas cannot be completely replacedThe former gas causes long adjustment time of system concentration and slow response; and (5) the system control is complex.
Therefore, there is a need for improvements in the art to provide a more reliable solution.
Disclosure of Invention
The technical problem to be solved by the present invention is to provide a three-gas multi-channel gas supply system and method for an incubator, aiming at the defects in the prior art.
In order to solve the technical problems, the invention adopts the technical scheme that: a three gas, multi-channel gas supply system for an incubator, comprising:
a gas source module including three gas source units for independently providing CO 2 、O 2 、N 2 Three pure gases;
the gas mixing module comprises three mass flow controllers, a mixing cavity and a pressure sensor, wherein the input ends of the three mass flow controllers are respectively connected with the three gas source units, the mixing cavity is connected with the output ends of the three mass flow controllers, and the pressure sensor is used for detecting the gas pressure in the mixing cavity;
cultivate room air feed module, its include with main air supply line that hybrid chamber is connected and parallelly connected the setting be in N air supply unit on the main air supply line, N air supply unit is used for independently providing CO for N cultivates the room 2 、O 2 And N 2 The gas supply unit comprises a sub gas supply pipeline connected with the main gas supply pipeline, a gas supply proportional valve and a gas supply flow sensor which are sequentially arranged on the sub gas supply pipeline along the gas flow direction, and the output end of the sub gas supply pipeline is communicated to the culture chamber;
and the gas detection module is communicated with the main gas supply pipeline and is arranged in parallel with the N gas supply units, and the gas detection module comprises a detection pipeline connected with the main gas supply pipeline and a detection proportional valve, a detection flow sensor, an oxygen concentration sensor, a carbon dioxide concentration sensor and a one-way valve which are arranged on the detection pipeline in sequence along the airflow direction.
Preferably, a pressure stabilizing valve is arranged on the main air supply pipeline at the downstream of the mixing cavity, and the air supply module and the air detection module of the culture chamber are both arranged at the downstream of the pressure stabilizing valve.
Preferably, the gas source unit comprises a gas storage container, a gas source pipeline communicating the gas storage container and the mass flow controller, and a gas source control valve arranged on the gas source pipeline.
Preferably, the opening and closing of the mass flow controller is feedback-controlled by a detection result of the pressure sensor, and when the pressure sensor detects that the pressure in the mixing chamber is lower than P1, the mass flow controller is opened and supplies gas into the mixing chamber at a flow rate of Q1; when the pressure sensor detects that the pressure in the mixing chamber is higher than P2, the mass flow controller is closed; wherein P1< P2.
Preferably, the detection proportional valve adopts an operation mode of intermittent opening, so that the oxygen concentration sensor and the carbon dioxide concentration sensor intermittently sample and detect the concentrations of oxygen and carbon dioxide in the gas output by the mixing cavity, when the concentration of oxygen or carbon dioxide exceeds a set range, the mass flow controller is opened to supplement new gas into the mixing cavity through the gas source module, and replace the original gas in the mixing cavity until the concentrations of oxygen and carbon dioxide in the gas discharged from the mixing cavity are both within the set range.
Preferably, a first filter is further disposed on the main air supply pipeline downstream of the mixing cavity, and the culture chamber air supply module and the gas detection module are both disposed downstream of the first filter.
Preferably, a second filter is further disposed between the supply air proportional valve and the supply air flow sensor.
Preferably, a humidifier is further arranged on the main gas supply pipeline downstream of the mixing chamber, and the culture chamber gas supply module and the gas detection module are both arranged downstream of the humidifier;
preferably, an ultraviolet sterilizer is further arranged on the main air supply pipeline at the downstream of the mixing cavity, and the culture room air supply module and the gas detection module are both arranged at the downstream of the ultraviolet sterilizer.
Preferably, the system supplies gas to the N culture chambers in the incubator by:
s1, setting the working pressure range of the mixing cavity to be P1-P2, and when the pressure sensor detects that the pressure in the mixing cavity is lower than P1, starting the mass flow controllers on the same air source unit and supplying air to the mixing cavity at the flow rate of Q1; when the pressure sensor detects that the pressure in the mixing cavity is higher than P2, the mass flow controller is closed, and the current air source unit stops supplying air to the mixing cavity;
s2, when the culture chamber is in a closed state, controlling the air supply proportional valve on the air supply unit corresponding to the culture chamber to supply air to the culture chamber at a flow q0;
when the culture chamber is in an open-cover state, closing a gas supply proportional valve on a gas supply unit corresponding to the culture chamber to stop gas supply of the culture chamber, after the culture chamber is closed, controlling the gas supply proportional valve on the gas supply unit corresponding to the culture chamber to control the gas supply flow to be qs, purging the culture chamber for time ts, and then controlling the flow q0 to supply gas to the culture chamber, wherein qs is more than q0;
s3, when the oxygen concentration sensor and the carbon dioxide concentration sensor detect that the concentration of oxygen or carbon dioxide in the gas output by the mixing cavity exceeds a set range Ct or the Ct needs to be changed, controlling the detection proportional valves and all gas supply proportional valves to output gas at a flow q1 so as to discharge the gas in the mixing cavity, simultaneously controlling three mass flow controllers to be opened and inputting gas into the mixing cavity according to the required gas concentration proportion, and continuing for a time t1 so as to replace the original gas in the mixing cavity; then the three mass flow controllers are closed, all the gas supply proportional valves supply gas to the corresponding culture chambers at the flow rate q0, and simultaneously the detection proportional valves recover the working mode of intermittent opening, wherein q1 is greater than q0;
and S4, when a certain culture room does not culture, controlling the air supply proportional valve on the air supply unit corresponding to the culture room to be closed so as to stop supplying air.
The invention has the beneficial effects that:
the invention can realize the on-line dynamic mixing and the quick concentration adjustment of three gases, and has the advantages of quick response, stable and accurate concentration control and simple control;
the culture room provided by the invention is provided with a plurality of gas supply channels and is expandable, and the culture rooms at the tail ends of the plurality of gas supply channels are independent from each other and do not influence each other during operation;
the invention can ensure that the gas concentration in each culture chamber is in the required range.
Drawings
FIG. 1 is a schematic diagram of a three-gas multi-channel gas supply system for an incubator according to the present invention.
Description of reference numerals:
1-an air source module; 10-gas storage container; 11-gas source pipeline; 12-gas source control valve;
2, a gas mixing module; 20-mass flow controller; 21-a mixing chamber; 22-a pressure sensor;
3-a gas detection module; 30-detecting a pipeline; 31-detection of the proportional valve; 32-a detection flow sensor; 33-oxygen concentration sensor; 34-carbon dioxide concentration sensor; 35-a one-way valve;
4-culture room air supply module; 40-a main gas supply line; 41-air supply proportional valve; 42-supply air flow sensor;
5-a pressure stabilizing valve;
6-culture chamber.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1
As shown in fig. 1, the present embodiment provides a three-gas multi-channel gas supply system for an incubator, comprising:
the gas source module (1) is provided with a gas source,it comprises three gas source units for respectively and independently providing CO 2 、O 2 、N 2 Three pure gases;
the gas mixing module 2 comprises three Mass Flow Controllers (MFCs) with input ends respectively connected with the three gas source units, a mixing cavity 21 connected with output ends of the three Mass Flow controllers 20 (MFC 1, MFC2 and MFC 3), and a pressure sensor 22 for detecting gas pressure in the mixing cavity 21;
the air supply module 4 of the culture room 6 comprises a main air supply pipeline 40 connected with the mixing cavity 21 and N air supply units arranged on the main air supply pipeline 40 in parallel, wherein the N air supply units are used for independently providing CO for the N culture rooms 6 2 、O 2 And N 2 The gas supply unit comprises a sub gas supply pipeline connected with the main gas supply pipeline 40, a gas supply proportional valve 41 and a gas supply flow sensor 42 which are sequentially arranged on the sub gas supply pipeline along the gas flow direction, and the output end of the sub gas supply pipeline is communicated to the culture chamber 6;
and the gas detection module 3 is communicated with the main gas supply pipeline 40 and is arranged in parallel with the N gas supply units, and the gas detection module 3 comprises a detection pipeline 30 connected with the main gas supply pipeline 40, and a detection proportional valve 31, a detection flow sensor 32, an oxygen concentration sensor 33, a carbon dioxide concentration sensor 34 and a one-way valve 35 which are sequentially arranged on the detection pipeline 30 along the gas flow direction. The oxygen concentration sensor 33 and the carbon dioxide concentration sensor 34 are used to detect other concentrations discharged from the mixing chamber 21, and the check valve 35 is open to the atmosphere.
In this embodiment, a pressure stabilizing valve 5 is arranged on the main gas supply line 40 at the downstream of the mixing cavity 21, and the gas supply module 4 and the gas detection module 3 of the culture chamber 6 are both arranged at the downstream of the pressure stabilizing valve 5. The pressure stabilizing valve 5 is used for ensuring the pressure at the front end of the air supply to ensure the stability of the air supply.
In this embodiment, the air source unit includes an air storage container 10, an air source pipeline 11 for communicating the air storage container 10 with the mass flow controller 20, and an air source control valve 12 (valve 1, valve 2, and valve 3) disposed on the air source pipeline 11.
Wherein, the opening and closing of the mass flow controller 20 is feedback controlled by the detection result of the pressure sensor 22, when the pressure sensor 22 detects that the pressure in the mixing cavity 21 is lower than P1, the mass flow controller 20 is opened, and supplies gas into the mixing cavity 21 with the flow rate of Q1; when the pressure sensor 22 detects that the pressure in the mixing chamber 21 is higher than P2, the mass flow controller 20 is closed; wherein P1< P2. For example, in one embodiment, P1 is 100kPa, P2 is 180kPa, and Q1 is 2000sccm.
Wherein, the detection proportional valve 31 adopts the working mode of intermittent opening, so as to intermittently sample and detect the concentration of oxygen and carbon dioxide in the gas output by the mixing cavity 21 through the oxygen concentration sensor 33 and the carbon dioxide concentration sensor 34, when the concentration of oxygen or carbon dioxide exceeds the set range, the mass flow controller 20 is opened to supplement new gas into the mixing cavity 21 through the gas source module 1, and replace the original gas in the mixing cavity 21 until the concentration of oxygen and carbon dioxide in the gas discharged from the mixing cavity 21 is in the set range.
In the preferred embodiment, a first filter (e.g., a HEPA filter) is also provided on main gas supply line 40 downstream of mixing chamber 21 to ensure the cleanliness of the gas entering each channel, and growth chamber 6 is downstream of both gas supply module 4 and gas detection module 3.
In the preferred embodiment, a second filter (e.g., a HEPA filter) is also provided between supply air proportional valve 41 and supply air flow sensor 42 to ensure the cleanliness of the air entering each growth chamber 6.
In the preferred embodiment, a humidifier is also provided on main gas supply line 40 downstream of mixing chamber 21 to humidify the gas entering growth chamber 6, and growth chamber 6 gas supply module 4 and gas detection module 3 are both downstream of the humidifier;
in the preferred embodiment, a UV sterilizer is also provided on main gas supply line 40 downstream of mixing chamber 21 to sterilize the gas entering growth chamber 6, and gas supply module 4 and gas detection module 3 for growth chamber 6 are both downstream of the UV sterilizer.
In a preferred embodiment, the gas from the cultivation room 6 is uniformly recovered or discharged through a channel to prevent the influence of the exhaust gas on the external environment or to ensure the safety in a high oxygen supply state.
Example 2
The present embodiment provides the method for supplying gas to N culture chambers 6 in an incubator by using the three-gas multi-channel gas supply system for an incubator of embodiment 1, specifically:
s1, setting the working pressure range of a mixing cavity 21 to be P1-P2 (such as 100-180 kPa), and when a pressure sensor 22 detects that the pressure in the mixing cavity 21 is lower than 100kPa, a mass flow controller 20 on the same gas source unit is opened and supplies gas into the mixing cavity 21 at the flow rate of Q1 (such as 2000 sccm); when the pressure sensor 22 detects that the pressure in the mixing chamber 21 is higher than 180kPa, the mass flow controller 20 is closed, and the front air supply unit stops supplying air to the mixing chamber 21;
s2, a normal mode: when the culture chamber 6 is in a closed state, the gas supply proportional valve 41 of the gas supply unit corresponding to the culture chamber 6 controls the supply of gas to the culture chamber 6 at a flow rate q0 (for example, 30 sccm);
purging mode: when the culture chamber 6 is in an open-lid state, the gas supply proportional valve 41 on the gas supply unit corresponding to the culture chamber 6 is closed to stop the gas supply to the culture chamber 6, and when the culture chamber 6 is closed, the gas supply proportional valve 41 on the gas supply unit corresponding to the culture chamber 6 controls the gas supply flow rate to be qs (for example, 300 sccm), purges the culture chamber 6 for a time ts (for example, 3 min), and then controls the flow rate q0 (for example, 30 sccm) to supply gas to the culture chamber 6;
s3, when the oxygen concentration sensor 33 and the carbon dioxide concentration sensor 34 detect that the concentration of oxygen or carbon dioxide in the gas output from the mixing chamber 21 exceeds a set range Ct or the Ct needs to be changed, controlling the detection proportional valve 31 and all the gas supply proportional valves 41 to output gas at a large flow q1 (for example, 300 sccm) so as to discharge the gas in the mixing chamber 21, and simultaneously controlling the three mass flow controllers 20 to be opened and input gas into the mixing chamber 21 according to the required gas concentration proportion for a time t1 (for example, 3 min) so as to replace the original gas in the mixing chamber 21, wherein in the process, the total flow of the three mass flow controllers 20 should be consistent with the total flow of the downstream detection proportional valves 31 and gas supply proportional valves 41 (for example, 1 detection proportional valve 31, 6 gas supply proportional valves 41, the flow of each proportional valve is 300sccm, and the total flow of the MFC is 2100 sccm); then, the three mass flow controllers 20 are closed, all the gas supply proportional valves 41 supply gas to the corresponding culture chambers 6 at a flow rate q0 (for example, 30 sccm), and the operation mode that the proportional valves 31 are intermittently opened is detected;
and S4, when a certain culture chamber 6 does not culture, controlling the air supply proportional valve 41 on the air supply unit corresponding to the culture chamber 6 to be closed so as to stop supplying air and save air.
While embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields of application of the invention, and further modifications may readily be effected by those skilled in the art, so that the invention is not limited to the specific details without departing from the general concept defined by the claims and the scope of equivalents.

Claims (10)

1. A three gas multichannel air supply system for incubator, comprising:
a gas source module including three gas source units for independently providing CO 2 、O 2 、N 2 Three pure gases;
the gas mixing module comprises three mass flow controllers, a mixing cavity and a pressure sensor, wherein the input ends of the three mass flow controllers are respectively connected with the three gas source units, the mixing cavity is connected with the output ends of the three mass flow controllers, and the pressure sensor is used for detecting the gas pressure in the mixing cavity;
cultivate room air feed module, its include with main air supply line that the hybrid chamber is connected and parallelly connected the setting be in N air feed unit on the main air supply line, N air feed unit is used for independently providing CO for N cultivates the room 2 、O 2 And N 2 The gas supply unit comprises a sub gas supply pipeline connected with the main gas supply pipeline, a gas supply proportional valve and a gas supply flow sensor which are sequentially arranged on the sub gas supply pipeline along the gas flow direction, and the output end of the sub gas supply pipeline is communicated to the culture chamber;
and the gas detection module, its with main gas supply line intercommunication and with the parallelly connected setting of a N air feed unit, the gas detection module include with the detection pipeline that main gas supply line connects and set gradually along the air current direction detect detection proportional valve, detection flow sensor, oxygen concentration sensor, carbon dioxide concentration sensor and check valve on the pipeline.
2. The three-gas multi-channel gas supply system for the incubator according to claim 1, wherein a pressure stabilizing valve is provided on the main gas supply pipeline at a downstream of the mixing chamber, and the incubator gas supply module and the gas detection module are both at a downstream of the pressure stabilizing valve.
3. The system of claim 2, wherein the gas source unit comprises a gas container, a gas source pipeline connecting the gas container and the mass flow controller, and a gas source control valve disposed on the gas source pipeline.
4. A biogas multi-channel gas supply system for an incubator according to claim 3, wherein the opening and closing of the mass flow controller is feedback-controlled by the detection result of the pressure sensor, and when the pressure sensor detects that the pressure in the mixing chamber is lower than P1, the mass flow controller is opened and supplies gas into the mixing chamber at a flow rate of Q1; when the pressure sensor detects that the pressure in the mixing chamber is higher than P2, the mass flow controller is closed; wherein P1< P2.
5. The multi-channel three-gas supply system for the incubator according to claim 4, wherein the detection proportional valve adopts an intermittently opened working mode to intermittently sample the concentrations of oxygen and carbon dioxide in the gas output from the mixing chamber through the oxygen concentration sensor and the carbon dioxide concentration sensor, and when the concentration of oxygen or carbon dioxide exceeds a set range, the mass flow controller is opened to supplement new gas into the mixing chamber through the gas source module and replace the original gas in the mixing chamber until the concentrations of oxygen and carbon dioxide in the gas output from the mixing chamber are both within the set range.
6. The system of claim 5, wherein a first filter is further disposed on the main gas supply line downstream of the mixing chamber, and the chamber gas supply module and the gas detection module are both downstream of the first filter.
7. The three-gas multi-channel gas supply system for the incubator according to claim 6, wherein a second filter is further provided between the gas supply proportional valve and the gas supply flow sensor.
8. The system of claim 7, wherein a humidifier is further provided on the main gas supply line downstream of the mixing chamber, and the chamber gas supply module and the gas detection module are both downstream of the humidifier.
9. The three-gas multi-channel gas supply system for the incubator according to claim 8, wherein an ultraviolet sterilizer is further provided on the main gas supply line downstream of the mixing chamber, and the incubator gas supply module and the gas detection module are both downstream of the ultraviolet sterilizer.
10. The biogas supply system for the incubator of claim 9, wherein the system supplies gas to N chambers in the incubator by:
s1, setting the working pressure range of the mixing cavity to be P1-P2, and when the pressure sensor detects that the pressure in the mixing cavity is lower than P1, starting the mass flow controllers on the same air source unit and supplying air to the mixing cavity at the flow rate of Q1; when the pressure sensor detects that the pressure in the mixing cavity is higher than P2, the mass flow controller is closed, and the front air source unit stops supplying air to the mixing cavity;
s2, when the culture chamber is in a closed state, controlling the air supply proportional valve on the air supply unit corresponding to the culture chamber to supply air to the culture chamber at a flow q0;
when the culture chamber is in an open-cover state, closing a gas supply proportional valve on a gas supply unit corresponding to the culture chamber to stop gas supply of the culture chamber, after the culture chamber is closed, controlling the gas supply proportional valve on the gas supply unit corresponding to the culture chamber to control the gas supply flow to be qs, purging the culture chamber for time ts, and then controlling the flow q0 to supply gas to the culture chamber, wherein qs is more than q0;
s3, when the oxygen concentration sensor and the carbon dioxide concentration sensor detect that the concentration of oxygen or carbon dioxide in the gas output by the mixing cavity exceeds a set range Ct or the Ct needs to be changed, controlling the detection proportional valves and all gas supply proportional valves to output gas at a flow q1 so as to discharge the gas in the mixing cavity, simultaneously controlling three mass flow controllers to be opened and inputting gas into the mixing cavity according to the required gas concentration proportion, and continuing for a time t1 so as to replace the original gas in the mixing cavity; then the three mass flow controllers are closed, all the gas supply proportional valves supply gas to the corresponding culture chambers at the flow rate q0, and simultaneously the detection proportional valves recover the working mode of intermittent opening, wherein q1 is greater than q0;
and S4, when a certain culture room does not culture, controlling the air supply proportional valve on the air supply unit corresponding to the culture room to be closed so as to stop supplying air.
CN202211336491.8A 2022-10-28 2022-10-28 Three-gas multi-channel gas supply system and method for incubator Pending CN115627214A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116606966A (en) * 2023-07-20 2023-08-18 中国科学院苏州生物医学工程技术研究所 Air inlet control method for three-gas supply system of cell incubator

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116606966A (en) * 2023-07-20 2023-08-18 中国科学院苏州生物医学工程技术研究所 Air inlet control method for three-gas supply system of cell incubator
CN116606966B (en) * 2023-07-20 2023-10-20 中国科学院苏州生物医学工程技术研究所 Air inlet control method for three-gas supply system of cell incubator

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