CN219709461U - Ciliate germplasm automatic preservation device - Google Patents
Ciliate germplasm automatic preservation device Download PDFInfo
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- CN219709461U CN219709461U CN202320257256.5U CN202320257256U CN219709461U CN 219709461 U CN219709461 U CN 219709461U CN 202320257256 U CN202320257256 U CN 202320257256U CN 219709461 U CN219709461 U CN 219709461U
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- 241000223782 Ciliophora Species 0.000 title claims abstract description 53
- 238000004321 preservation Methods 0.000 title claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 79
- 239000002699 waste material Substances 0.000 claims abstract description 33
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims abstract description 25
- 230000000712 assembly Effects 0.000 claims abstract description 14
- 238000000429 assembly Methods 0.000 claims abstract description 14
- 210000003437 trachea Anatomy 0.000 claims abstract description 6
- 230000000149 penetrating effect Effects 0.000 claims description 5
- 230000002265 prevention Effects 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 6
- 230000001954 sterilising effect Effects 0.000 description 9
- 108010066278 cabin-4 Proteins 0.000 description 7
- 239000007789 gas Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 6
- 238000012258 culturing Methods 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- 238000011109 contamination Methods 0.000 description 4
- 238000012864 cross contamination Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 210000004602 germ cell Anatomy 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000005949 ozonolysis reaction Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/90—Protozoa ; Processes using protozoa
Abstract
The utility model discloses an automatic ciliate germplasm preservation device. The preservation device comprises a seed preservation structure, at least three groups of five-way valve assemblies, a ciliate culture assembly and a control system. The seed protection structure comprises a culture solution barrel, a main liquid inlet pipe, a waste liquid tank, a circulating fan and an ozone generator. Wherein, the culture solution barrel is provided with an air inlet, and an air pipe is fixedly connected between the air inlet and the ozone generator. Each set of five-way valve assemblies includes a valve body and a valve spool. Wherein, the valve body is provided with an A port, a B port, a C port, a D port and an E port; the E mouth department fixedly connected with five-way valve trachea of valve body, and five-way valve trachea internal fixation has the HEPA filter. The control system comprises a PLC, a waste liquid cabin electromagnetic valve and a balance port electromagnetic valve arranged on the culture solution barrel. The utility model can reduce the pollution of other non-food bacteria to the ciliate culture system, effectively maintain the stability of the seed-protecting system, reduce the workload of artificial passage seed-protecting and reduce the seed-protecting cost.
Description
Technical Field
The utility model relates to the technical field of ciliate germplasm resource preservation, in particular to an automatic ciliate germplasm preservation device.
Background
Ciliates are single-cell protozoa, have the characteristics of wide distribution and cell nucleus binaryzation, and are important research materials for ecology, cell biology, evolutionary biology and other subjects. Unlike bacteria, fungi and other microbes, most ciliates cannot be stably and conveniently frozen or dried powder preserved, and the ciliate germplasm preservation is carried out by using a traditional container such as a test tube, a culture dish and the like and subculturing in a manual passage mode.
However, conventional seed conservation operations are time-consuming and labor-consuming, and the intermediate process often introduces cross-contamination between different species or other non-food bacterial contamination, causing confusion or loss of the species.
Disclosure of Invention
Based on the above, it is necessary to provide an automatic ciliate germplasm preservation device for solving the problems that when the current seed preservation operation is time-consuming and labor-consuming, cross contamination among different germlines or other non-food bacterial contamination is often introduced in the middle process, so that the germline is disordered or lost.
An automated ciliate germplasm preservation device comprising:
the seed protection structure comprises a culture solution barrel, a main liquid inlet pipe, a waste liquid tank, a circulating fan communicated with the waste liquid tank and an ozone generator communicated with the circulating fan; wherein, the culture solution barrel is provided with an air inlet, and an air pipe is fixedly connected between the air inlet and the ozone generator;
at least three sets of five-way valve assemblies for receiving the culture solution sent through the main liquid inlet pipe; each group of five-way valve assembly comprises a valve body and a valve core positioned in the valve body;
the ciliate culture component receives the culture solution sent by the corresponding five-way valve component; and
the control system comprises a PLC, a waste liquid cabin electromagnetic valve and a balance port electromagnetic valve arranged on the culture solution barrel;
wherein, the valve body is provided with an A port, a B port, a C port, a D port and an E port; the E mouth department fixedly connected with five-way valve trachea of valve body, and five-way valve trachea internal fixation has the HEPA filter.
The preservation device can reduce pollution of other non-food bacteria to the ciliate culture system, effectively maintain stability of the seed preservation system, reduce workload of artificial passage seed preservation and reduce seed preservation cost.
In one embodiment, a barrel cover is movably arranged on the culture solution barrel; the liquid inlet main pipe is fixedly connected to the liquid outlet of the culture liquid barrel.
In one embodiment, a touch screen is fixedly installed on the PLC; the control system also comprises a frequency converter, a relay I and a relay II; the frequency converter is used for controlling the rotating speed of the circulating fan, and the waste liquid cabin electromagnetic valve is fixedly arranged at a waste liquid outlet of the waste liquid cabin; and the first relay and the second relay are used for controlling the opening and closing of the five-way valve assembly.
In one embodiment, the valve core is rotatably installed relative to the valve body; the five-way valve assembly is used for providing pollution prevention protection for the corresponding ciliate culture assembly;
each group of five-way valve assembly further comprises a shell, a motor, a first travel switch, a second travel switch, a pinion, a driving shaft, a large gear and a stop block, wherein the motor is fixedly arranged in the shell, the first travel switch and the second travel switch, the pinion is fixedly arranged at the position of the output end of the motor, the driving shaft is positioned in the shell, the large gear is fixedly arranged on the driving shaft and meshed with the pinion, and the stop block is fixedly arranged on the driving shaft.
Further, when the valve core rotates to the closing position, the stop block touches the first travel switch, at the moment, the first travel switch is disconnected, the motor is powered off and stops rotating, and the valve core stays at the closing position; when the valve core rotates to the opening position, the stop dog touches the travel switch II, at the moment, the travel switch II is disconnected, the motor is powered off and stops rotating, and the valve core stays at the opening position.
Still further, the deflection angle of the stop block is 90 degrees when the stop block moves from the first travel switch to the second travel switch.
In one embodiment, the housing is fixedly mounted at a rear surface location of the valve body; one end of the driving shaft is vertically and movably connected between the valve body and the shell in a penetrating way; the valve core is fixedly arranged at the end position of the driving shaft, which is opposite to the stop block.
Further, a five-way valve liquid inlet pipe is fixedly connected between the port A of the valve body and the liquid inlet main pipe, a five-way valve liquid outlet pipe is fixedly connected between the port B of the valve body and the waste liquid cabin, a culture bottle liquid inlet pipe is fixedly connected between the port C of the valve body and the corresponding ciliate culture assembly, and a culture bottle liquid outlet pipe is fixedly connected between the port D of the valve body and the corresponding ciliate culture assembly.
Still further, each set of the ciliate culturing assemblies comprises a compartment, a culture flask positioned within the compartment;
a rubber plug is fixedly arranged at the bottle opening position of the culture bottle; the culture bottle liquid inlet pipe and the culture bottle liquid outlet pipe are fixedly connected with the corresponding rubber plugs in a penetrating mode.
In one embodiment, in the first state, the valve core is located at an opening position, and at this time, the port a is communicated with the port C, and the port B is communicated with the port D;
in the second state, the valve core is positioned at the closing position, at the moment, the port A is communicated with the port B, and the port C, the port D and the port E are communicated.
Compared with the prior art, the utility model has the beneficial effects that:
the method can effectively improve the quality of the ciliate seed preservation, maintain a stable culture environment, avoid the condition of disordered or lost seed lines caused by cross contamination among different seed lines or other non-food bacterial contamination introduced in the middle process of the traditional seed preservation operation, greatly reduce the manual operation and the labor amount, reduce the seed preservation cost, can be widely applied to a large-scale and automatic seed preservation system, and has excellent popularization prospect.
Drawings
Fig. 1 is a schematic structural view of an automatic ciliate germplasm preserving device according to the present utility model.
Fig. 2 is a schematic diagram of the five-way valve assembly of fig. 1.
Fig. 3 shows a partial side view of fig. 2.
FIG. 4 is a schematic view of the valve cartridge of FIG. 1 rotated to an open position.
FIG. 5 is a schematic view of the valve cartridge of FIG. 1 rotated to a closed position.
Fig. 6 is a block diagram showing a ciliate germplasm automatic preserving apparatus according to the present utility model.
Fig. 7 is a flowchart showing an automated ciliate germplasm preserving apparatus according to the present utility model.
Description of the main reference signs
1. A culture solution barrel; 2. a balance port electromagnetic valve; 3. a liquid inlet main pipe; 4. a waste liquid tank; 5. a circulating fan; 6. an ozone generator; 7. a partition box; 8. a culture bottle; 9. a valve body; 10. a valve core; 11. a housing; 12. a motor; 13. a travel switch I; 14. a travel switch II; 15. a pinion gear; 16. a drive shaft; 17. a large gear; 18. a stop block; 19. a five-way valve liquid inlet pipe; 20. a five-way valve drain pipe; 21. a five-way valve air pipe; 22. HEPA filter.
The foregoing general description of the utility model will be described in further detail with reference to the drawings and detailed description.
Detailed Description
The present utility model will be described in detail with reference to the accompanying drawings.
Referring to fig. 1, the present embodiment provides an automated ciliate germplasm preserving apparatus, which includes a seed retaining structure, three sets of five-way valve assemblies, a ciliate culturing assembly, and a control system. In this embodiment, three sets of two-position five-way valve assemblies are taken as an example for illustration, and in other embodiments, the number of five-way valve assemblies can be adaptively increased according to the actual seed-retaining scale, and the corresponding numbers of ciliate culture assemblies are also increased synchronously.
The seed protection structure comprises a culture solution barrel 1, a main liquid inlet pipe 3, a waste liquid cabin 4, a circulating fan 5 communicated with the waste liquid cabin 4 and an ozone generator 6 communicated with the circulating fan 5. The culture solution barrel 1 is movably provided with a barrel cover, and a filling port of the culture solution is closed based on the barrel cover. The liquid inlet main pipe 3 is fixedly connected to the liquid outlet of the culture liquid barrel 1. Wherein, an air inlet is arranged on the culture solution barrel 1, and an air pipe is fixedly connected between the air inlet and the ozone generator 6. When the balance port electromagnetic valve 2 is opened, the air pressure in the culture solution barrel 1 is equal to the atmospheric pressure, so that on one hand, the positive pressure in the passage can be eliminated, and on the other hand, the liquid in the waste liquid cabin 4 can be smoothly discharged, and the balance port electromagnetic valve 2 is closed during circulation sterilization.
The five-way valve assembly receives the culture solution fed through the main liquid inlet pipe 3 and plays a role in providing anti-pollution protection for the corresponding ciliate culture assembly. With continued reference to fig. 2, each set of five-way valve assembly includes a valve body 9, a valve spool 10 positioned within the valve body 9, a housing 11, a motor 12 fixedly mounted within the housing 11, a first and second travel switches 13 and 14, a pinion 15 fixedly mounted at an output end position of the motor 12, a drive shaft 16 positioned within the housing 11, a large gear 17 fixedly mounted on the drive shaft 16 and engaged with the pinion 15, and a stop 18 fixedly mounted on the drive shaft 16. The shell 11 is fixedly arranged at the position of the rear surface of the valve body 9, one end of the driving shaft 16 is vertically and movably connected between the valve body 9 and the shell 11 in a penetrating way, and the valve core 10 is fixedly arranged at the position of the end part of the driving shaft 16, which is opposite to the stop block 18.
The valve core 10 is rotatably mounted with respect to the valve body 9. In this embodiment, the motor 12 drives the pinion 15 to rotate, and under the meshing action, the pinion 15 drives the bull gear 17 to rotate, so as to achieve the purpose of driving the valve core 10 and the stop block 18 to rotate. When the valve core 10 rotates to the closing position, the stop block 18 contacts the travel switch I13, at this time, the travel switch I13 is disconnected, the motor 12 is powered off and stopped, and the valve core 10 stays at the closing position. When the valve core 10 rotates to the opening position, the stop block 18 contacts the travel switch II 14, at this time, the travel switch II 14 is disconnected, the motor 12 is powered off and stopped, and the valve core 10 stays at the opening position. Referring to fig. 3, the stop 18 is deflected by 90 ° when moving from the first position of the first travel switch 13 to the second position of the second travel switch 14. In this embodiment, the first travel switch 13 or the second travel switch 14 is connected to the motor 12 in series, and the first travel switch 13 and the second travel switch 14 are normally closed switches.
Wherein, opening A mouth, B mouth, C mouth, D mouth and E mouth on the valve body 9. A five-way valve liquid inlet pipe 19 is fixedly connected between the A port of the valve body 9 and the liquid inlet main pipe 3, a five-way valve liquid discharge pipe 20 is fixedly connected between the B port of the valve body 9 and the liquid waste cabin 4, a culture bottle liquid inlet pipe is fixedly connected between the C port of the valve body 9 and the corresponding ciliate culture assembly, and a culture bottle liquid discharge pipe is fixedly connected between the D port of the valve body 9 and the corresponding ciliate culture assembly.
The ciliate culturing assemblies receive the culture fluid fed through the corresponding five-way valve assemblies, and each set of ciliate culturing assemblies comprises a separation box 7 and a culturing bottle 8 positioned in the separation box 7. In this embodiment, the mounting position of the culture flask 8 is higher than that of the culture solution tank 1, so that the overflow phenomenon of the liquid does not occur when the problems such as the blockage of the pipeline or the aging of the sealing member occur. The bottle mouth position of the culture bottle 8 is fixedly provided with a rubber plug, and the culture bottle liquid inlet pipe and the culture bottle liquid outlet pipe are fixedly connected with the corresponding rubber plugs in a penetrating way. The E mouth of the valve body 9 is fixedly connected with a five-way valve air pipe 21, and a HEPA filter 22 is fixedly installed in the five-way valve air pipe 21, in this embodiment, when the culture flask 8 is in a closed state, only the E mouth of the valve body 9 is used for exchanging gas with the outside, and in the process, the effect of preventing pollution caused by invasion of mixed bacteria is achieved based on the HEPA filter 22.
The valve core 10 of this embodiment is provided with a fan-shaped notch, which has the advantages that an outlet E is added, part of ciliates need to consume oxygen during cultivation, and carbon dioxide and other gases can be generated, and when the valve is closed, the culture bottle 8 cannot be completely closed, and needs to be communicated with the outside, so the E-port is added. Referring to fig. 4 in combination, when ciliates are cultured, the five-way valve assembly is in an open state, the valve core 10 is in an open position, and at this time, the port a is communicated with the port C, and the port B is communicated with the port D.
Referring to fig. 5 again, in the long-term cultivation process, the C port and the D port of the valve core 10 may be attached by ciliates, so that sterilization treatment is required, the valve core 10 is turned to the closed position, at this time, the a port and the B port are communicated, the C port, the D port and the E port are communicated, the culture solution tank 1, the five-way valve assembly, the waste solution tank 4, the circulating fan 5, the ozone generator 6 and the pipelines among the parts form a closed circulating loop, and the circulating fan 5 extracts ozone generated by the ozone generator 6 and circulates in the device by means of ozone to achieve the effect of removing pollutants.
The embodiment can maintain the stability of the culture environment based on the treatment, avoid cross contamination among different systems or other non-food bacterial contamination introduced in the middle of the traditional seed preservation operation, and achieve the aim of effectively improving the seed preservation quality of ciliates.
Aiming at the current situation that part of contaminants such as miscellaneous bacteria are attached to the pipe wall, clean water flushing cannot ensure complete removal, liquid bactericide is remained in the pipeline and possibly enters the culture pipe to kill ciliates, the preservation device of the embodiment introduces ozone for circulation sterilization, when the valve core 10 is in the closed position, the culture solution barrel 1, the five-way valve assembly, the waste liquid cabin 4, the circulating fan 5, the ozone generator 6 and the pipeline among the parts form a closed circulation loop, the circulating fan 5 extracts sterilizing gas (ozone) generated by the ozone generator 6, the sterilizing gas (ozone) circulates in the device by virtue of the sterilizing gas (ozone) to achieve the effect of killing the contaminants, and the sterilizing gas is not discharged into a laboratory, has the advantage of not polluting the environment, and is added with liquid after the ozone is automatically degraded.
Referring to fig. 6, the control system includes a PLC, a waste liquid tank solenoid valve, and a balance port solenoid valve 2, a frequency converter, a relay one and a relay two provided on the culture liquid tank 1. The touch screen is fixedly installed on the PLC, in the embodiment, instructions are sent to each execution terminal based on the PLC as a central controller so as to ensure orderly work of seed protection equipment, the touch screen is matched as a man-machine interaction window, and an automatic liquid change button connected with the PLC and independent buttons of each execution mechanism are assembled on the touch screen to send instructions of operators.
A waste tank solenoid valve is fixedly installed at a waste liquid outlet of the waste tank 4 for controlling opening/closing of a waste liquid discharge passage. The relay I and the relay II are used for controlling the opening and closing of the five-way valve assembly. In this embodiment, the rotation speed of the fan 5 is circulated through the frequency converter, the first relay supplies power to the five-way valve assembly in a forward direction, the second relay supplies power to the five-way valve assembly in a reverse direction, the first travel switch 13 and the second travel switch 14 are connected in series therebetween, and in the power supply process, the first travel switch 13 and the second travel switch 14 are triggered by the stop block 18, so that the purpose of stopping the valve core 10 when the power supply is disconnected is achieved.
With continued reference to fig. 7, the preservation device of the present embodiment operates as follows:
s1, opening a barrel cover, and closing the barrel cover after filling culture solution;
s2, opening a five-way valve assembly to culture and preserve ciliate germplasm, and opening a balance port electromagnetic valve 2 to enable the culture solution barrel 1 to be the same as the atmospheric pressure, so that positive pressure is prevented from being generated when the circulating fan 5 is started;
s3, starting a circulating fan 5, and providing negative pressure for the culture bottle by the circulating fan 5 to extract the culture solution in the culture solution barrel 1 until the culture solution in the culture solution barrel 1 is evacuated;
s4, closing the five-way valve assembly to perform ozone circulation sterilization, wherein the culture bottle 8 is in a closed state, and only exchanges gas with the outside through an E port of the valve core 10 and the HEPA filter 22, and the circulating fan 5 continuously operates to pump residual liquid in the pipeline into the waste liquid cabin 4;
s5, closing the circulating fan 5;
s6, opening a waste liquid tank electromagnetic valve, and discharging waste liquid in the waste liquid tank 4;
s7, closing a waste liquid cabin electromagnetic valve, and closing a balance port electromagnetic valve 2 to enable pipelines among the culture liquid barrel 1, the five-way valve assembly, the waste liquid cabin 4, the circulating fan 5, the ozone generator 6 and all parts to form a closed loop;
s8, starting a circulating fan 5, starting an ozone generator 6, and circulating ozone in the loop;
s9, after the set time is reached, the circulating fan 5 is turned off, and the ozone generator 6 is turned off;
so far, the liquid exchange and sterilization are completed, and after the liquid exchange is completed, the control system is automatically locked until the set ozonolysis time is reached.
In summary, the preservation device of the present embodiment has the following advantages compared to the preservation measures of the conventional ciliate germplasm: the preservation device of this embodiment can reduce the pollution of other non-food bacteria to ciliate culture system, effectively maintains the stability of guarantor's kind of system, reduces artificial passaging guarantor's work load and guarantor's kind cost.
The naming of the components involved is based on the functions described in the specification as naming standards, and is not limited by the specific terms used in the present utility model, and other terms may be selected by those skilled in the art to describe the names of the components of the present utility model.
Claims (10)
1. An automated ciliate germplasm preservation device, comprising:
the seed protection structure comprises a culture solution barrel (1), a main liquid inlet pipe (3), a waste liquid tank (4), a circulating fan (5) communicated with the waste liquid tank (4) and an ozone generator (6) communicated with the circulating fan (5); wherein, the culture solution barrel (1) is provided with an air inlet, and an air pipe is fixedly connected between the air inlet and the ozone generator (6);
at least three sets of five-way valve assemblies for receiving the culture solution sent through the main liquid inlet pipe (3); each group of five-way valve assemblies comprises a valve body (9) and a valve core (10) positioned in the valve body (9);
the ciliate culture component receives the culture solution sent by the corresponding five-way valve component; and
the control system comprises a PLC, a waste liquid cabin electromagnetic valve and a balance port electromagnetic valve (2) arranged on the culture solution barrel (1);
wherein, the valve body (9) is provided with an A port, a B port, a C port, a D port and an E port; the E mouth department of valve body (9) fixedly connected with five-way valve trachea (21), and five-way valve trachea (21) internal fixation is equipped with HEPA filter (22).
2. An automated ciliate germplasm preserving apparatus according to claim 1, wherein: a barrel cover is movably arranged on the culture solution barrel (1); the liquid inlet main pipe (3) is fixedly connected to the liquid outlet of the culture liquid barrel (1).
3. An automated ciliate germplasm preserving apparatus according to claim 1, wherein: a touch screen is fixedly arranged on the PLC; the control system also comprises a frequency converter, a relay I and a relay II;
the frequency converter is used for controlling the rotating speed of the circulating fan (5), and the waste liquid cabin electromagnetic valve is fixedly arranged at a waste liquid outlet of the waste liquid cabin (4); and the first relay and the second relay are used for controlling the opening and closing of the five-way valve assembly.
4. An automated ciliate germplasm preserving apparatus according to claim 1, wherein: the valve core (10) is rotatably arranged relative to the valve body (9); the five-way valve assembly is used for providing pollution prevention protection for the corresponding ciliate culture assembly;
each group of five-way valve assembly further comprises a shell (11), a motor (12), a first travel switch (13) and a second travel switch (14), a pinion (15), a driving shaft (16), a large gear (17) and a stop block (18), wherein the motor (12) is fixedly arranged in the shell (11), the first travel switch (13) and the second travel switch (14), the pinion (15) is fixedly arranged at the output end position of the motor (12), the driving shaft (16) is arranged in the shell (11), the large gear (17) is fixedly arranged on the driving shaft (16) and meshed with the pinion (15), and the stop block (18) is fixedly arranged on the driving shaft (16).
5. An automated ciliate germplasm preserving apparatus according to claim 4, wherein: when the valve core (10) rotates to a closing position, the stop block (18) contacts the travel switch I (13), at the moment, the travel switch I (13) is disconnected, the motor (12) is powered off and stopped, and the valve core (10) stays at the closing position;
when the valve core (10) rotates to the opening position, the stop block (18) contacts the travel switch II (14), at the moment, the travel switch II (14) is disconnected, the motor (12) is powered off and stopped, and the valve core (10) stays at the opening position.
6. An automated ciliate germplasm preserving apparatus according to claim 5, characterized in that said stop (18) is deflected by 90 ° when moving from said first (13) to said second (14) position.
7. An automated ciliate germplasm preserving apparatus according to claim 4, wherein: the shell (11) is fixedly arranged at the position of the rear surface of the valve body (9);
one end of the driving shaft (16) is vertically and movably connected between the valve body (9) and the shell (11); the valve core (10) is fixedly arranged at the end position of the driving shaft (16) opposite to the stop block (18).
8. The automatic ciliate germplasm preserving device according to claim 7, wherein a five-way valve liquid inlet pipe (19) is fixedly connected between an A port of the valve body (9) and the liquid inlet main pipe (3), a five-way valve liquid outlet pipe (20) is fixedly connected between a B port of the valve body (9) and the waste liquid tank (4), a culture bottle liquid inlet pipe is fixedly connected between a C port of the valve body (9) and a corresponding ciliate culture component, and a culture bottle liquid outlet pipe is fixedly connected between a D port of the valve body (9) and the corresponding ciliate culture component.
9. An automated ciliate germplasm preserving device according to claim 8, characterized in that each set of said ciliate culture assemblies comprises a compartment box (7), a culture flask (8) located inside said compartment box (7);
a rubber plug is fixedly arranged at the bottle opening position of the culture bottle (8); the culture bottle liquid inlet pipe and the culture bottle liquid outlet pipe are fixedly connected with the corresponding rubber plugs in a penetrating mode.
10. An automated ciliate germplasm preserving device according to claim 1, characterized in that in the first state the valve cartridge (10) is in an open position, in which port a communicates with port C and port B communicates with port D;
in the second state, the valve core (10) is positioned at a closing position, at the moment, the port A is communicated with the port B, and the port C, the port D and the port E are communicated.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210728127X | 2022-06-24 | ||
| CN202210728127.XA CN115044456A (en) | 2022-06-24 | 2022-06-24 | Be applied to automation equipment that large-scale germplasm of ciliate was preserved |
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| CN219709461U true CN219709461U (en) | 2023-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210728127.XA Pending CN115044456A (en) | 2022-06-24 | 2022-06-24 | Be applied to automation equipment that large-scale germplasm of ciliate was preserved |
| CN202320257256.5U Active CN219709461U (en) | 2022-06-24 | 2023-02-20 | Ciliate germplasm automatic preservation device |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210728127.XA Pending CN115044456A (en) | 2022-06-24 | 2022-06-24 | Be applied to automation equipment that large-scale germplasm of ciliate was preserved |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4225924B2 (en) * | 2004-01-19 | 2009-02-18 | 三洋電機株式会社 | Automatic subculture apparatus and subculture method using the same |
| WO2017056142A1 (en) * | 2015-09-28 | 2017-04-06 | 株式会社日立ハイテクノロジーズ | Flow passage module and cell culture apparatus using same |
| WO2017163326A1 (en) * | 2016-03-23 | 2017-09-28 | 株式会社日立製作所 | Culture container and automatic culture device using same |
| CN107725831A (en) * | 2017-11-17 | 2018-02-23 | 成都市同展食品有限公司 | A kind of four-way commutation and self-choke plunger valve |
| JP2021048777A (en) * | 2019-09-20 | 2021-04-01 | 株式会社島津製作所 | Cell culture system and cell culture method |
| CN212560287U (en) * | 2020-05-30 | 2021-02-19 | 双桥（厦门）有限公司 | Feeding and discharging structure of heterogeneous column |
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