CN110669668A

CN110669668A - Carbon dioxide incubator

Info

Publication number: CN110669668A
Application number: CN201911019188.3A
Authority: CN
Inventors: 庞逢祥; 杨超; 张博韬; 赵臻; 张立明; 温邵博
Original assignee: Qinhuangdao Zhaojian Biotechnology Co Ltd
Current assignee: Qinhuangdao Zhaojian Biotechnology Co Ltd
Priority date: 2019-10-24
Filing date: 2019-10-24
Publication date: 2020-01-10
Anticipated expiration: 2039-10-24
Also published as: CN110669668B

Abstract

The invention relates to a carbon dioxide incubator, which comprises an incubator body, incubator wheels and a control panel, wherein the incubator body is divided into a plurality of independent cavities, and an outer incubator door is hinged outside each independent cavity; an inner cavity is arranged in each independent cavity; each surface of the inner side of the partition plate of the inner cavity is covered with a heat insulation layer; each inner cavity is provided with an inner glass door and is provided with an independent temperature control system, a carbon dioxide concentration control system, a constant humidity control system and a sterilization and disinfection device. The invention has the beneficial effects that: the culture of different conditions in the same incubator is realized, intercellular cross contamination is avoided, the equipment investment cost of a plurality of incubators is saved, and the heat dissipation function is also increased. And the cells are not influenced by the fluctuation of the external environment due to the intermediate disinfection and sterilization operation in the culture process, the operation is simple, the cell culture is not interrupted, and the cell culture efficiency is improved.

Description

Carbon dioxide incubator

Technical Field

The invention relates to the field of biological incubators, in particular to a carbon dioxide incubator.

Background

The carbon dioxide incubator realizes the in-vitro culture of cells by simulating the formation of a growth environment similar to that in an organism in an incubator body. When the carbon dioxide incubator is used, stable temperature (37 ℃) and stable CO in the incubator body are required₂The level (5%), the constant pH value (pH value: 7.2-7.4) and the higher relative saturation humidity (95%), and is widely applied to the culture of cells, tissues and some special microorganisms.

However, the conventional carbon dioxide incubator cannot simultaneously culture cells under different culture conditions because the internal conditions are uniform. Therefore, to culture cells under different conditions, a plurality of incubators are required, which is costly and takes up a large space. On the other hand, the heating effect of the existing carbon dioxide incubator is concentrated, so that the temperature distribution of the culture area is not uniform, a constant environment is difficult to provide for cell culture, and the feedback of the temperature sensor is influenced. And the carbon dioxide incubator among the prior art only heats the function, because open the chamber door and can cause the cell pollution also do not possess the heat dissipation function, when the inside device of incubator generates heat, the temperature in the incubator will receive the influence, causes the unstability. In addition, when the sterilization and disinfection operation is performed again during the culture process, the cultured cells need to be taken out, and thus the cell culture is interrupted and is hardly affected by the fluctuation of the external environment.

Disclosure of Invention

Technical problem to be solved

The invention provides a carbon dioxide incubator, which solves the problems that in the prior art, a single carbon dioxide incubator is difficult to simultaneously cultivate cells under different culture conditions, the cultured cells are fluctuated and polluted by re-sterilization and disinfection in the opening and closing process and during the culture process of a box door, the heating effect is not uniform, the carbon dioxide incubator does not have a heat dissipation function, and the temperature floats.

(II) technical scheme

In order to achieve the purpose, the invention adopts the main technical scheme that:

a carbon dioxide incubator comprises an incubator body, incubator wheels and a control panel, wherein the incubator body is divided into two parts, the upper part is a regulation and control area, and the lower part is an incubation area; the culture area is divided into a plurality of independent cavities by partition plates;

the side edge of the independent cavity is hinged with an outer box door, an inner cavity is arranged in the independent cavity, the inner cavity and the independent cavity are arranged in a non-contact manner, and the side edge of the inner cavity is hinged with an inner glass door;

each inner cavity is provided with an independent temperature control system, a carbon dioxide concentration control system and a constant humidity control system; the temperature control system, the carbon dioxide concentration control system and the constant humidity control system are integrated in the regulation and control area, and a regulation and control area shell is covered outside the regulation and control area;

the temperature control system comprises copper pipes fixed on the outer sides of all the surfaces of the inner cavity;

a square-shaped plate is arranged between the independent cavity and the inner cavity, and the square-shaped plate and the inner glass door are positioned on the same plane; when the outer box door is in an open state, the independent cavity is still a closed area due to the arrangement of the square-shaped return plate.

The carbon dioxide concentration control system comprises a carbon dioxide pipe joint, a pressure reducing valve and a carbon dioxide conveying pipe; the carbon dioxide conveying pipe comprises a carbon dioxide conveying main pipe and a plurality of carbon dioxide conveying branch pipes;

the pressure reducing valve is arranged on the carbon dioxide conveying main pipe, one end of the carbon dioxide conveying branch pipe is connected with the carbon dioxide conveying main pipe, and the other end of the carbon dioxide conveying branch pipe is connected with the carbon dioxide throttle valve on the outer side of the rear wall surface of each inner cavity;

the pressure reducing valve is arranged in the regulating and controlling area shell, and a pressure regulating window is arranged at the position of the regulating and controlling area shell corresponding to the pressure reducing valve;

furthermore, the carbon dioxide pipe joint is externally connected with a carbon dioxide source.

The constant humidity control system comprises a water injection port, an atomization generator and a mist conveying pipe in the middle of the top of the regulation and control area;

the mist conveying pipe comprises a mist conveying main pipe and a plurality of mist conveying branch pipes, one end of the atomization generator is connected with the water injection port, and the other end of the atomization generator is connected with the mist conveying main pipe; and a mist throttle valve is arranged below each carbon dioxide throttle valve and is connected with the mist conveying branch pipe.

Each surface in each inner cavity is attached with a heat insulation layer; each surface of each inner cavity is provided with a plurality of narrow and long pores.

Furthermore, silicon rubber sealing strips are padded between the inner glass door and the inner cavity and between the outer box door and the independent cavity.

Furthermore, a copper pipe is also fixed on the inner side of each outer box door.

Furthermore, an ultraviolet lamp is arranged on the top wall of each inner cavity.

Furthermore, a ventilation fan is arranged on the rear wall surface of each inner cavity.

Furthermore, two side walls of the inner cavity are provided with laminate supporting plates perpendicular to the bottom surface, and the laminate supporting plates are provided with a plurality of grooves in the horizontal direction.

Furthermore, every the top of interior cavity is provided with the sensor safety cover, be provided with carbon dioxide concentration sensor, humidity transducer, temperature sensor in the sensor safety cover.

Further, the copper pipe is filled with heating liquid or steam.

(III) advantageous effects

The invention has the beneficial effects that:

1. through setting up a plurality of interior cavities, realized cultivateing the culture of different conditions in same incubator, avoided intercellular cross contamination, saved the equipment input cost of a plurality of incubators simultaneously.

2. The culture can be transferred to the inner cavity with proper culture conditions in time, the cells are not influenced by the fluctuation of the external environment due to the intermediate disinfection and sterilization operation in the culture process, the cell culture is not interrupted, and the cell culture efficiency is improved.

3. The carbon dioxide concentration, the temperature, the humidity and the disinfection and sterilization operation of each inner cavity can be independently controlled, and the cost investment is not increased.

4. Through the setting of returning the word shaped plate, realized among the prior art the heat dissipation function that the carbon dioxide incubator did not possess, and easy operation only needs to open outer container door and can realize, needn't worry the pollution problem.

5. Six-side heating can be realized in the culture area of the inner cavity, so that heating is more uniform and faster.

Drawings

FIG. 1 is a schematic view of the overall structure of a carbon dioxide incubator;

FIG. 2 is a schematic view of the overall structure of a carbon dioxide incubator;

FIG. 3 is a rear view of the carbon dioxide incubator;

FIG. 4 is a schematic view of the structure of the inner cavity of the carbon dioxide incubator;

FIG. 5 is a schematic view of the rear side wall of the inner cavity of the carbon dioxide incubator.

[ description of reference ]

101: a control panel; 102: a control button; 103: an outer box door; 104: a separate chamber;

201: a chassis housing; 202: a carbon dioxide pipe joint; 203: a voltage regulating window; 204: a pressure reducing valve; 205: a water injection port; 206: a box wheel; 207: a regulatory region envelope;

301: a glass inner door; 302: an inner cavity; 303: a copper pipe; 304: an ultraviolet lamp; 305: a sensor protection cover; 306: laminating the board; 307: a silicone rubber sealing strip; 308: a laminate support plate;

401: a ventilating fan; 402: a mist throttle valve; 403: a carbon dioxide throttle valve; 404: a mist delivery pipe; 405: a carbon dioxide conveying pipe.

Detailed Description

For the purpose of better explaining the present invention and to facilitate understanding, the present invention will be described in detail by way of specific embodiments with reference to the accompanying drawings.

A carbon dioxide incubator, as shown in figure 1 and figure 2, comprises a box body, a control panel 101 and box wheels 206, wherein the box body is divided into two parts, the upper part is a regulation area, and the lower part is a culture area; the box body is divided into a plurality of independent cavities 104 by partition plates, and the side edge of each independent cavity 104 is hinged with an outer box door 103; an inner cavity 302 is arranged in the independent cavity 104, the inner cavity 302 and the independent cavity 104 are arranged in a non-contact mode, and the side edge of the inner cavity 302 is hinged with an inner glass door 301;

each inner cavity 302 is provided with an independent temperature control system, a carbon dioxide concentration control system and a constant humidity control system; the temperature control system, the carbon dioxide concentration control system and the constant humidity control system are integrated in the regulation and control area, and a regulation and control area shell 207 is covered outside the regulation and control area;

the temperature control system comprises copper pipes 303 fixed on the outer sides of all the surfaces of the inner cavity 302; heating liquid or gas is introduced into the copper pipe 303, and heat is supplied to the inner cavity through heat conduction of the copper pipe. Be equipped with temperature sensor in the interior cavity 302, can in time feed back the inside temperature variation of cavity, and then provide the constant temperature environment.

Further, copper tubes 303 are also fixed to the inner side of the outer box door 103, thus realizing six-sided heating of the culture in the inner cavity 302. Compared with the prior art, six surfaces of the culture cavity are heated to ensure that the heat is distributed uniformly and intensively, the heating process is quicker and more efficient, and the problems that the temperature distribution is uneven in the culture area, and the temperature feedback by the sensor is not representative and the like are avoided.

the pressure reducing valve 204 is arranged on the carbon dioxide conveying main pipe, one end of the carbon dioxide conveying branch pipe is connected with the carbon dioxide conveying main pipe, and the other end of the carbon dioxide conveying branch pipe is connected with the carbon dioxide throttle valve 403 on the outer side of the rear wall surface of each inner cavity 302;

the pressure reducing valve is arranged in the regulating and controlling area shell, and a pressure regulating window 203 is arranged at the position of the regulating and controlling area shell 207 corresponding to the pressure reducing valve 204; a carbon dioxide concentration sensor is arranged in the inner cavity 302;

further, the carbon dioxide pipe joint 202 is externally connected with a carbon dioxide source.

The carbon dioxide pressure reducing valve 204 reduces the carbon dioxide to a proper working pressure; the carbon dioxide exits the pressure relief valve 204 and passes through the carbon dioxide delivery manifold and then through the carbon dioxide delivery manifold associated with each of the internal chambers 302 to the internal chambers 302. The carbon dioxide throttle valve 403 can control the circulation and interruption of carbon dioxide in each inner chamber 302, and is independent of each other, thereby realizing independent control of carbon dioxide in each inner chamber 302. In addition, the carbon dioxide concentration in the culture region can be monitored in real time by the carbon dioxide sensor in each inner cavity 302, and the concentration of carbon dioxide in the culture region can be maintained in a constant state.

The constant humidity control system comprises a water injection port 205 in the middle of the top of the regulation and control area, an atomization generator, a mist conveying pipe 404 and a humidity sensor; a water source is connected outside the water injection port 205;

the mist delivery pipe 404 comprises a mist delivery main pipe and a plurality of mist delivery branch pipes, one end of the atomization generator is connected with the water injection port 205, and the other end of the atomization generator is connected with the mist delivery main pipe; a mist throttle valve 402 is provided below each of the carbon dioxide throttle valves 403, and the mist throttle valve 402 is connected to the mist delivery branch pipe. The atomization generator changes water into mist, which meets the requirement of culture conditions; the mist throttle valve 404 may independently control the replenishment and interruption of water vapor in each internal cavity according to different requirements, thereby maintaining the humidity of one or more internal cavities 302 constant.

Further, an ultraviolet lamp 304 is disposed on a top wall of each of the inner chambers 302. During the cell or tissue culture process, the culture area needs to be sterilized again to prevent the outside environment from polluting the culture. However, since the carbon dioxide incubator in the prior art has only one culture area, the culture is often required to be placed in the external environment when the re-sterilization and disinfection operation is performed in the culture process. Therefore, on one hand, the external environment is difficult to meet the culture conditions, so that the environment fluctuation is caused, and the culture is influenced; on the other hand, the outside environment outside the incubator is likely to contaminate the culture.

In the present invention, the operator can perform the sterilization operation on any inner cavity 302 at any time without worrying about the influence of environmental fluctuation and external pollution in the culture process. The operator only needs to transfer the cell tissue and the like being cultured into another inner cavity with proper carbon dioxide, temperature and humidity and disinfection and sterilization, so that the culture is not interrupted, the culture is not polluted, and the quality of the culture can be improved.

A square-shaped plate 105 is arranged between the independent cavity 104 and the inner cavity 302, and the square-shaped plate 105 and the inner glass door 301 are positioned on the same plane; when the outer box door 103 is in an open state, the independent cavity 104 is still an enclosed space due to the return-shaped plate 105. The square-back plate 105 can prevent foreign substances from entering the cell culture area to cause the pollution of culture substances. When some parts in the incubator are used for a long time and generate heat to enable the temperature to rise, the heat dissipation operation can be achieved only by opening the outer box door 301, external pollution cannot be caused, and due to the six-face heating system, even if the outer box door 301 is opened, heat dissipation cannot cause fluctuation to the environmental condition of the incubator.

Each surface in each inner cavity 302 is attached with a heat insulation layer to ensure the concentration of heat; each surface of each inner cavity is provided with a plurality of narrow and long pores, the pores enable carbon dioxide and water vapor required in the cell culture process to permeate through the pores, and the distribution of the carbon dioxide, the water vapor and the like in the culture environment is uniform.

Further, silicone rubber sealing strips are padded between the inner glass door 301 and the inner cavity 302 and between the outer box door 103 and the independent cavity 104.

Further, a sensor protection cover 305 is arranged on the top of each inner cavity 302, and a carbon dioxide concentration sensor, a humidity sensor and a temperature sensor are arranged in the sensor protection cover 305.

Further, every set up ventilation fan 401 on the interior cavity rear wall, when cell culture, open ventilation fan 401, the air evenly flows in whole box, and then drives carbon dioxide, humidity, heat etc. including in cavity 302 evenly distributed for the culture condition is more idealized.

Furthermore, two side walls of the inner cavity 302 are provided with laminate support plates 308 perpendicular to the bottom surface, and a plurality of grooves in the horizontal direction are formed in the laminate support plates 308. When the number of cells or tissues to be cultured is large, a plurality of laminates 306 can be placed and clamped in the grooves of the laminate supporting plate 308, so that the culture number is increased, and the space utilization rate is improved.

The carbon dioxide incubator in the prior art is only suitable for culturing the cultures of the same type, and if the cultures of different types are placed in the unified incubator, intercellular cross contamination can be caused. The invention arranges a plurality of inner cavities in one incubator, can simultaneously cultivate different types of cultures, does not need to additionally increase the equipment investment cost, simultaneously avoids cross contamination and improves the cultivation efficiency.

The above embodiments are merely illustrative, and not restrictive, of the scope of the invention, and those skilled in the art will be able to make various changes and modifications within the scope of the appended claims without departing from the spirit of the invention.

Claims

1. A carbon dioxide incubator comprises an incubator body, incubator wheels (206) and a control panel (101), and is characterized in that the incubator body is divided into two parts, wherein the upper part is a regulation area, and the lower part is a culture area; the culture area is divided into a plurality of independent cavities (104) by partition plates;

the side edge of the independent cavity (104) is hinged with an outer box door (103), an inner cavity (302) is arranged in the independent cavity (104), the inner cavity (302) and the independent cavity (104) are arranged in a non-contact mode, and the side edge of the inner cavity (302) is hinged with an inner glass door (301);

each inner cavity (302) is provided with an independent temperature control system, a carbon dioxide concentration control system and a constant humidity control system; the temperature control system, the carbon dioxide concentration control system and the constant humidity control system are integrated in the regulation and control area, and a regulation and control area shell (207) is covered outside the regulation and control area;

the temperature control system comprises copper pipes (303) fixed on the outer sides of all the surfaces of the inner cavity (302);

a square-shaped plate (105) is arranged between the independent cavity (104) and the inner cavity (302), and the square-shaped plate (105) and the inner glass door (301) are positioned on the same plane; when the outer box door (103) is in an open state, the independent cavity (104) is still a closed area due to the arrangement of the return-shaped plate (105).

2. A carbon dioxide incubator according to claim 1, wherein the carbon dioxide concentration control system comprises a carbon dioxide pipe connection (202), a pressure reducing valve (204), a carbon dioxide delivery pipe (405); the carbon dioxide conveying pipe (405) comprises a carbon dioxide conveying main pipe and a plurality of carbon dioxide conveying branch pipes;

the pressure reducing valve (204) is arranged on the carbon dioxide conveying main pipe, one end of each carbon dioxide conveying branch pipe is connected with the carbon dioxide conveying main pipe, and the other end of each carbon dioxide conveying branch pipe is connected with a carbon dioxide throttle valve (403) on the outer side of the rear wall surface of each inner cavity (302);

the pressure reducing valve (204) is arranged in the regulating and controlling area shell (207), and a pressure regulating window (203) is arranged at the position of the regulating and controlling area shell corresponding to the pressure reducing valve (204);

the carbon dioxide pipe joint (202) is externally connected with a carbon dioxide source.

3. The carbon dioxide incubator of claim 1, wherein the constant humidity control system comprises a water injection port (205) in the middle of the top of the conditioning area, an atomization generator, a mist delivery pipe (404);

the mist conveying pipe (404) comprises a mist conveying main pipe and a plurality of mist conveying branch pipes, one end of the atomization generator is connected with the water injection port, and the other end of the atomization generator is connected with the mist conveying main pipe; a mist throttle valve (402) is provided below each of the carbon dioxide throttle valves (403), the mist throttle valve (402) being connected to the mist delivery branch pipe.

4. A carbon dioxide incubator according to claim 1, characterised in that a thermal insulating layer is attached to each face within each said inner chamber (302); each surface of each inner cavity (302) is provided with a plurality of narrow and long pores.

And silicon rubber sealing strips are padded between the inner glass door (301) and the inner cavity (302) and between the outer box door (103) and the independent cavity (104).

5. A carbon dioxide incubator according to claim 1, characterised in that a copper tube (303) is also secured to the inside of each outer door (103).

6. The carbon dioxide incubator as recited in claim 1, characterized in that an ultraviolet lamp is provided on a top wall of each of said inner chambers (302).

7. The carbon dioxide incubator as claimed in claim 1, wherein a ventilation fan (401) is provided on a rear wall surface of each of the inner chambers (302).

8. The carbon dioxide incubator as claimed in claim 1, wherein two side walls of the inner chamber (302) are provided with a laminate support plate (308) perpendicular to the bottom surface, and the laminate support plate (308) is provided with a plurality of grooves in the horizontal direction.

9. The carbon dioxide incubator according to claim 1, wherein a sensor protection cover (305) is arranged on the top of each inner cavity (302), and a carbon dioxide concentration sensor, a humidity sensor and a temperature sensor are arranged in the sensor protection cover (305).

10. Carbon dioxide incubator according to claim 1, characterised in that the copper tube (305) is internally ventilated with a heating liquid or steam.