CN220537825U - Cell culture device - Google Patents
Cell culture device Download PDFInfo
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- CN220537825U CN220537825U CN202322040243.5U CN202322040243U CN220537825U CN 220537825 U CN220537825 U CN 220537825U CN 202322040243 U CN202322040243 U CN 202322040243U CN 220537825 U CN220537825 U CN 220537825U
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- cell culture
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- 238000004113 cell culture Methods 0.000 title claims abstract description 64
- 239000011521 glass Substances 0.000 claims abstract description 23
- 239000000463 material Substances 0.000 claims description 8
- 239000004793 Polystyrene Substances 0.000 claims description 7
- 229920002223 polystyrene Polymers 0.000 claims description 7
- 238000012856 packing Methods 0.000 claims description 2
- 238000003384 imaging method Methods 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 6
- 238000010186 staining Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 21
- 238000002474 experimental method Methods 0.000 description 6
- 230000009194 climbing Effects 0.000 description 4
- 230000001464 adherent effect Effects 0.000 description 3
- 238000004891 communication Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000002834 transmittance Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000010788 consumable waste Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture device, which comprises a glass slide, wherein a plurality of sunken cell culture areas are arranged on the upper end surface of the glass slide at intervals, an auxiliary device is arranged above the cell culture areas, a plurality of through holes are arranged on the upper end surface of the auxiliary device, the through holes are respectively arranged corresponding to the cell culture areas, gaskets are respectively arranged at the connection parts of the cell culture areas and the through holes, the thickness of the bottom of each cell culture area is 0.17mm, the condition that culture solution is mutually leaked between the cell culture areas after the auxiliary device is buckled on the glass slide can be prevented, and then, the simultaneous culture of multiple types of cells can be realized, the requirements of subsequent cell staining and imaging can be directly met, the operation process is simplified, and the utility model belongs to the field of cell culture and imaging.
Description
Technical Field
The utility model belongs to the field of cell culture and imaging, and particularly relates to a cell culture device.
Background
Cells are fundamental units that make up the morphology and function of organisms, and are composed of many parts, and the exploration of genes, protein functions, and physiological and disease processes by life sciences and medicine is largely based on cell-level expansion.
Through putting the slide in the cell culture plate, soaking in the cell culture medium, let the cell grow on the slide, and then be applied to experiments such as histology, immunohistochemistry, frozen section, cell smear and in situ hybridization, current cell climbing piece has four specifications that the diameter is 8mm, 14mm, 20mm and 25mm, is applicable to 48 orifice plate, 24 orifice plate, 12 orifice plate and 6 orifice plate respectively, when the experiment is accomplished, need take out the slide from the shrinkage pool of cell culture plate with tweezers and seal the back of piece but imaging detection, so only can cultivate a cell alone in every culture dish hole, cell adsorbs only a cell on the climbing piece, and then a climbing piece only can seal on a slide.
Therefore, each glass slide can only carry out staining imaging of one cell type, so that consumable waste is caused, the complaining process involves a plurality of operation steps, the operation difficulty is high, the operation is complex, and the risk of cell pollution exists when the climbing slides are placed and taken out.
Disclosure of Invention
The utility model mainly aims to provide a cell culture device which can realize simultaneous culture and subsequent imaging of multiple cell types and is simple in operation process.
In order to achieve the above purpose, the technical scheme of the utility model is as follows:
the cell culture device comprises a glass slide, a plurality of sunken cell culture areas are arranged on the upper end face of the glass slide at intervals, an auxiliary device is arranged above the cell culture areas, a plurality of through holes are formed in the upper end face of the auxiliary device, the through holes are respectively and correspondingly arranged with the cell culture areas, gaskets are respectively arranged at the joints of the cell culture areas and the through holes, and the thickness of the bottoms of the cell culture areas is 0.17mm.
In a specific embodiment of the present utility model, a cavity capable of accommodating the glass slide is provided in the auxiliary device, and the through holes are respectively communicated with the cavity.
In a specific embodiment of the utility model, a cover plate is arranged above the auxiliary device.
In a specific embodiment of the present utility model, the cover plate is a polystyrene material member.
In a specific embodiment of the present utility model, the inner wall and the bottom surface of each cell culture section are provided with TC treatment layers.
In a particular embodiment of the utility model, the auxiliary device is a polystyrene material member.
In a particular embodiment of the utility model, the slide is a glass member.
One of the above technical solutions of the present utility model has at least one of the following advantages or beneficial effects:
according to the utility model, the plurality of cell culture areas are arranged at intervals on the upper end surface of the glass slide, the auxiliary device is arranged above the cell culture areas, the plurality of through holes are arranged on the upper end surface of the auxiliary device, the through holes are respectively arranged corresponding to the cell culture areas to form a plurality of culture chambers, and further gaskets are respectively arranged at the joints of the cell culture areas and the through holes, so that the condition that culture solutions are mutually exposed between the cell culture areas after the auxiliary device is buckled on the glass slide can be prevented, and further, the simultaneous culture of multiple types of cells can be realized, the bottom thickness of each cell culture area is set to be 0.17mm, thus the requirements of subsequent cell dyeing and imaging can be directly met, and the operation process is simplified.
Drawings
The utility model is further described below with reference to the drawings and examples;
FIG. 1 is an exploded view of one embodiment of the present utility model;
FIG. 2 is a top view of a slide according to one embodiment of the utility model;
FIG. 3 is a cross-sectional view in the direction of slide A-A in accordance with one embodiment of the utility model;
FIG. 4 is a top view of an auxiliary device according to one embodiment of the present utility model;
fig. 5 is a sectional view in the direction B-B of an auxiliary device according to an embodiment of the present utility model.
Detailed Description
Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present utility model and are not to be construed as limiting the present utility model.
In the description of the present utility model, it should be understood that references to orientation descriptions such as upper, lower, front, rear, left, right, etc. are based on the orientation or positional relationship shown in the drawings, are merely for convenience in describing the simplified description of the present utility model, and do not indicate or imply that the apparatus or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present utility model.
In the description of the present utility model, a number means one or more, a number means two or more, and greater than, less than, exceeding, etc. are understood to not include the present number, and above, below, within, etc. are understood to include the present number. The description of the first and second is for the purpose of distinguishing between technical features only and should not be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, features defining "first" and "second" may explicitly or implicitly include one or more features.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the term "connected" should be construed broadly, and for example, it may be a fixed connection or an active connection, or it may be a detachable connection or a non-detachable connection, or it may be an integral connection; may be mechanically connected, may be electrically connected, or may be in communication with each other; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements, indirect communication or interaction relationship between the two elements.
The following disclosure provides many different embodiments, or examples, for implementing different aspects of the utility model.
Referring to fig. 1 to 5, a cell culture device, including slide glass 1, slide glass 1 up end interval is equipped with three cell culture district 3 that are used for holding cell culture liquid, cell culture district 3 is the sunk area, can make mutual isolation between the cell culture district 3, cell culture district 3 top is equipped with auxiliary device 4, auxiliary device 4 up end is equipped with three through-hole 5, each through-hole 5 corresponds the setting with cell culture district 3 respectively, the junction of cell culture district 3 and through-hole 5 is equipped with packing ring 6 respectively, the thickness of each cell culture district 3 bottom is 0.17mm, can directly carry out subsequent dyeing and imaging work, the efficiency of cell biological detection has been improved.
In a specific embodiment of the present utility model, by placing a cell culture solution in the cell culture sections 3 to culture different types of cells in each cell culture section 3, by disposing three through holes 5 corresponding to the three cell culture sections 3, respectively, the three cell culture sections 3 and the three through holes 5 form three culture chambers, respectively, which do not affect each other, and by disposing gaskets 6 at the junctions of the cell culture sections 3 and the through holes 5, respectively, it is possible to prevent the cell culture solution from flowing out of the cell culture sections 3.
In a specific embodiment of the utility model, a cavity 7 capable of accommodating the glass slide 1 is arranged in the auxiliary device 4, the through holes 5 are respectively communicated with the cavity 7, the auxiliary device 4 can be buckled on the glass slide 1 when experiments are carried out through arranging the cavity 7, and further three cell culture areas 3 and three through holes 5 can respectively form three culture chambers for culturing and observing different cell lines.
In a specific embodiment of the present utility model, the glass member of the slide glass 1 can ensure the light transmittance and heat resistance of the slide glass 1, so as to meet the requirement of the subsequent scanning imaging step.
In a specific embodiment of the utility model, TC treatment layers are arranged on the inner wall and the bottom surface of each cell culture zone 3, and the cell culture modes mainly comprise adherent cells and suspension cells, wherein the suspension cells are independent of the surface of a support and grow in a suspension state in a culture solution, such as lymphocytes; adherent cells are those on which the growth of cells must be supported by attachment, on which surface the growth, proliferation, or both of which are dependent on the attachment factor secreted by itself or provided in the medium, is a major part of the animal cells.
In order to introduce hydrophilic factors on the surfaces of the inner walls and the bottom surfaces of the three cell culture regions 3, the inner walls and the bottom surfaces of the three cell culture regions 3 are subjected to surface modification treatment, namely TC treatment, so as to adapt to the growth and propagation of adherent cells.
In a particular embodiment of the utility model, a cover plate 2 is arranged above the auxiliary device 4, the cover plate 2 being similar in size to the auxiliary device 4, so that impurities can be prevented from entering the cell culture zone 3 during the experiment.
In a specific embodiment of the present utility model, the cover plate 2 is made of polystyrene material, so that the cover plate 2 can have good light transmittance and rigidity.
In a specific embodiment of the present utility model, since the polystyrene material member has good light transmittance and rigidity, the auxiliary device 4 is made of polystyrene material, so that the requirement that the auxiliary device 4 needs to be fastened on the glass slide 1 during the experiment can be met, and the observation of the experiment process is facilitated.
While embodiments of the present utility model have been shown and described, it will be understood by those of ordinary skill in the art that: many changes, modifications, substitutions and variations may be made to the embodiments without departing from the spirit and principles of the utility model, the scope of which is defined by the claims and their equivalents.
Claims (7)
1. The utility model provides a cell culture device, includes slide glass (1), its characterized in that, slide glass (1) up end interval is equipped with a plurality of sunken cell culture district (3), cell culture district (3) top is equipped with auxiliary device (4), auxiliary device (4) up end is equipped with a plurality of through-holes (5), each through-hole (5) respectively with cell culture district (3) correspond the setting, cell culture district (3) with the junction of through-hole (5) is equipped with packing ring (6) respectively, each thickness in cell culture district (3) bottom is 0.17mm.
2. The cell culture apparatus of claim 1, wherein: the auxiliary device (4) is internally provided with a cavity (7) capable of accommodating the glass slide (1), and the through holes (5) are respectively communicated with the cavity (7).
3. The cell culture apparatus of claim 2, wherein: a cover plate (2) is arranged above the auxiliary device (4).
4. A cell culture apparatus according to claim 3, wherein: the cover plate (2) is a polystyrene material member.
5. The cell culture apparatus of claim 1, wherein: TC treatment layers are arranged on the inner wall and the bottom surface of each cell culture zone (3).
6. The cell culture apparatus of claim 1, wherein: the auxiliary device (4) is a polystyrene material member.
7. The cell culture apparatus of claim 1, wherein: the glass slide (1) is a glass material component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322040243.5U CN220537825U (en) | 2023-07-31 | 2023-07-31 | Cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322040243.5U CN220537825U (en) | 2023-07-31 | 2023-07-31 | Cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220537825U true CN220537825U (en) | 2024-02-27 |
Family
ID=89962953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322040243.5U Active CN220537825U (en) | 2023-07-31 | 2023-07-31 | Cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220537825U (en) |
-
2023
- 2023-07-31 CN CN202322040243.5U patent/CN220537825U/en active Active
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