CN219363670U

CN219363670U - Detachable culture plate capable of preventing cells from falling off

Info

Publication number: CN219363670U
Application number: CN202320327877.6U
Authority: CN
Inventors: 王丽洋; 叶凯霞; 丁科林; 郑康; 王健; 童道颖
Original assignee: Zhejiang Boyu Biotechnology Co ltd
Current assignee: Zhejiang Boyu Biotechnology Co ltd
Priority date: 2023-02-16
Filing date: 2023-02-16
Publication date: 2023-07-18
Anticipated expiration: 2033-02-16

Abstract

The utility model relates to the technical field of biological culture dishes, and relates to a detachable culture plate for preventing cells from falling, which comprises a cell culture plate base, a culture dish and a culture plate cover plate, wherein the cell culture plate base is in a cuboid dish shape, six culture dish clamping grooves are formed in the cell culture plate base, the culture dish clamping grooves are slightly larger than the culture dish and have the same structure, the culture dish consists of a culture groove and a sample adding buffer groove, the sample adding buffer groove is obliquely connected and arranged at the bottom of the culture groove, and the culture plate cover plate is in fit with and cover on the cell culture plate base. According to the utility model, the cell culture plate base and the culture dish are arranged to be detachable, so that the cyclic utilization of the culture plate main body is realized, and only the culture dish in the groove is replaced; by adding the sample adding buffer groove, the impact force during liquid adding is relieved, and the probability of cell scattering is reduced.

Description

Detachable culture plate capable of preventing cells from falling off

Technical Field

The utility model relates to the technical field of biological culture dishes, in particular to a detachable culture plate capable of preventing cells from falling off.

Background

Cell culture (cell culture) is a method of simulating in vitro an in vivo environment (sterile, suitable temperature, ph, certain nutritional conditions, etc.) to survive, grow, reproduce and maintain a main structure and function, and is an important and common technique in cell biology research methods, by which a large number of cells can be obtained, and by which signal transduction of cells, anabolism of cells, growth and proliferation of cells, etc. can be studied.

The patent number CN217677579U discloses a cell culture dish capable of being disassembled to provide various splicing modes, which comprises a plurality of culture dish split bodies which are disassembled to be connected, wherein each culture dish split body comprises a substrate, a plurality of culture holes are formed in the substrate, a buffer liquid exchange hole is formed in the outer side of each culture hole of the substrate, and the bottom of each buffer liquid exchange hole is communicated with the bottom of each culture hole; any one of the culture dish split bodies is clamped with a plurality of adjacent culture dish split bodies. The buffer liquid exchange hole can be connected with the gun head, and the culture liquid is injected into the buffer liquid exchange hole through the gun head during daily liquid exchange, so that the impact of the liquid on bottom cells during liquid exchange is effectively reduced, the cell floating caused by operation is prevented to a certain extent, and the problems that the cell adhesion is difficult during osteogenesis induction and the cell floating is easy to cause during daily liquid exchange are solved. In addition, the adjacent culture dishes are connected in a split manner through the clamping connection, so that the cell culture dishes are formed, and the experimenters can conveniently splice the adjacent culture dishes in groups according to the experimental design.

The above-mentioned cell culture dish that can dismantle and provide multiple concatenation mode has following problem: the culture dish is not completely independent; in the liquid exchange process, an independent instrument is needed to assist, so that the working efficiency of operators is reduced.

Disclosure of Invention

The utility model provides a detachable culture plate for preventing cells from falling off, which solves the problems in the prior art.

The aim of the utility model can be achieved by the following technical scheme: a removable culture plate for preventing cell shedding comprising: cell culture plate base, culture dish and culture plate apron, cell culture plate base is cuboid form of dish, and inside is provided with six culture dish draw-in grooves, the culture dish draw-in groove is slightly greater than the culture dish, and both structures are unanimous, the culture dish comprises culture tank and application of sample buffer tank, the setting of application of sample buffer tank slope is in culture tank bottom, the culture plate apron agrees with the lid on the cell culture plate base.

Further improvement, the lower extreme connection of application of sample buffer tank is provided with the valve.

Further improved, a composite block is arranged at the opening of the upper end of the sample adding buffer groove.

Further improvements, grooves are also arranged in the cell culture plate base.

Further improvement, be provided with circular location blind hole all around the cell culture board base, integrated into one piece is provided with the locating pin all around the culture board apron lower extreme, circular location blind hole with locating pin quantity is the same and the position is corresponding.

Further improvement, the lower end of the culture plate cover plate is also provided with an annular sealing port which is slightly larger than the dish ports of the culture dishes, and the annular sealing ports and the culture dishes are the same in number and correspond in position.

Further improvement, the upper end of the culture plate cover plate is also provided with a handle.

Compared with the prior art, the utility model has the following beneficial effects:

1) And (3) the assembly and disassembly are carried out: according to the utility model, each culture dish of the cell culture plate is independently separated, and after one culture dish is used, the culture plate can be continuously used by replacing a new culture dish, that is, the culture plate main body can be manufactured by using other materials (ceramic and glass) without affecting microscopic observation, so that the cyclic utilization of the culture plate main body is realized, and only the culture dish in the groove is replaced.

2) Preventing the liquid exchange cells from falling off: in the process of liquid exchange, the adherent cells are easy to wash down by liquid absorption and liquid addition, so that the cell shedding directly affects the follow-up experiment progress.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a schematic diagram of a culture dish clamping groove according to the utility model;

FIG. 3 is a schematic view of the structure of the culture dish according to the present utility model;

FIG. 4 is a schematic view of the structure of the cover plate of the culture plate of the utility model.

In the figure, 1, a cell culture plate base; 11. a culture dish clamping groove; 12. a groove; 13. a circular positioning blind hole; 2. a culture dish; 21. a culture tank; 22. a sample adding buffer groove; 221. a valve; 222. a composite block; 3. a culture plate cover plate; 31. a positioning pin; 32. an annular sealing port; 33. a handle.

Detailed Description

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

In the description of the present utility model, it should be noted that the terms "mounted," "connected," and "coupled" are to be construed broadly, as well as, for example, fixedly coupled, detachably coupled, or integrally coupled, unless otherwise specifically indicated and defined. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

The technical scheme of the utility model is further described below with reference to the examples and the accompanying figures 1-4.

Example 1

As shown in fig. 1 to 4, a detachable culture plate capable of preventing cells from falling off comprises a cell culture plate base 1, a culture dish 2 and a culture plate cover plate 3, wherein the cell culture plate base 1 is in a cuboid dish shape, six culture dish clamping grooves 11 are formed in the cell culture plate base 1, the culture dish clamping grooves 11 are slightly larger than the culture dish 2, the culture dish clamping grooves are identical in structure, the culture dish 2 consists of a culture groove 21 and a sample adding buffer groove 22, the sample adding buffer groove 22 is obliquely connected and arranged at the bottom of the culture groove 21, and the culture plate cover plate 3 is in fit with and covers on the cell culture plate base 1.

The culture plate comprises a base clamping groove and separated culture dishes 2, when the conventional cell culture plate is used, if the culture dishes 2 are invalid due to misoperation (such as wrong reagent addition or wrong reagent amount), a new culture plate is generally replaced for rigorous experiments at the moment to cause loss, each culture dish 2 is independent, if some culture dishes 2 cannot be used for some reasons, the culture dishes 2 can be replaced to be newly arranged on the culture plate, and other usable culture dishes 2 can be continuously used, so that loss can be reduced.

As a further preferable embodiment, a valve 221 is connected to the lower end of the sample loading buffer tank 22, and the valve 221 can be closed after the sample loading is completed to prevent the liquid from flowing back.

As a further preferred embodiment, the opening at the upper end of the sample loading buffer slot 22 is provided with a composite block 222, which can be compressed when inserted into the sample loading buffer slot 22 and restored when pulled out, thereby playing a role in isolating pollution.

By combining the technical scheme, when cells are cultured by the traditional cell culture plate, the adherent cells are easily washed down by liquid suction and liquid adding in the liquid exchange process, so that the cell shedding affects the experiment. The sample adding buffer groove 22 is arranged on the outer side of the independent culture dish, so that liquid slowly flows into the culture dish 2 during liquid adding, the falling rate of adherent cells is reduced, and meanwhile, the pollution risk caused by adding one part is also realized by the composite block 222 and the valve 221 of the sample adding buffer groove 22.

As a further preferred embodiment, the cell culture plate base 1 is also provided with a groove 12, and in use, sterile water is added to relieve evaporation of liquid in the culture wells.

As a further preferred embodiment, the cell culture plate base 1 is provided with round locating blind holes 13 all around, integrated into one piece is provided with locating pins 31 all around at the culture plate apron 3 lower extreme, round locating blind holes 13 with locating pins 31 quantity is the same and the position is corresponding, can prevent through round locating blind holes 13 and locating pins 31 that culture plate apron 3 from taking place the displacement when receiving external force, influences the accuracy of experiment.

As a further preferable embodiment, the lower end of the culture plate cover plate 3 is further provided with an annular sealing port 32, the annular sealing port 32 is slightly larger than the dish 2 ports of the culture dish, the annular sealing ports 32 and the culture dish 2 are the same in number and corresponding in position, sealing protection of the culture dish 2 is achieved through the annular sealing ports 32, and accuracy of experiments is improved.

As a further preferred embodiment, the upper end of the culture plate cover plate 3 is also provided with a handle 33, which facilitates the opening and closing of the culture plate cover plate 3.

The foregoing describes in detail preferred embodiments of the present utility model. It should be understood that numerous modifications and variations can be made in accordance with the concepts of the utility model by one of ordinary skill in the art without undue burden. Therefore, all technical solutions which can be obtained by logic analysis, reasoning or limited experiments based on the prior art by the person skilled in the art according to the inventive concept shall be within the scope of protection defined by the claims.

Claims

1. A removable culture plate for preventing cell shedding comprising: cell culture board base (1), culture dish (2) and culture plate apron (3), cell culture board base (1) is cuboid form of a dish, and inside is provided with six culture dish draw-in grooves (11), culture dish draw-in groove (11) are greater than slightly culture dish (2), and both structures are unanimous, culture dish (2) are constituteed by culture tank (21) and application of sample buffer tank (22), application of sample buffer tank (22) slope connection sets up culture tank (21) bottom, culture plate apron (3) agree with the lid on cell culture board base (1).

2. The detachable culture plate for preventing cell shedding according to claim 1, wherein a valve (221) is connected to the lower end of the sample loading buffer tank (22).

3. The detachable culture plate for preventing cell detachment according to claim 2, characterized in that a composite block (222) is arranged at the opening of the upper end of the sample loading buffer tank (22).

4. A removable culture plate for preventing cell detachment according to claim 3, characterized in that the cell culture plate base (1) is further provided with a recess (12).

5. The detachable culture plate for preventing cell shedding according to claim 4, wherein round positioning blind holes (13) are formed in the periphery of the cell culture plate base (1), positioning pins (31) are integrally formed in the periphery of the lower end of the culture plate cover plate (3), and the number of the round positioning blind holes (13) and the number of the positioning pins (31) are the same and the positions of the round positioning blind holes are corresponding to each other.

6. The detachable culture plate for preventing cell shedding according to claim 5, wherein an annular sealing port (32) is further arranged at the lower end of the culture plate cover plate (3), the annular sealing port (32) is slightly larger than the dish port of the culture dish (2), and the annular sealing ports (32) and the culture dish (2) are the same in number and correspond in position.

7. The detachable culture plate for preventing cell detachment according to claim 6, wherein the upper end of the culture plate cover plate (3) is further provided with a handle (33).