CN210945622U - Primary culture dish of mesenchymal stem cells - Google Patents
Primary culture dish of mesenchymal stem cells Download PDFInfo
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- CN210945622U CN210945622U CN201920857399.3U CN201920857399U CN210945622U CN 210945622 U CN210945622 U CN 210945622U CN 201920857399 U CN201920857399 U CN 201920857399U CN 210945622 U CN210945622 U CN 210945622U
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Abstract
The utility model relates to a culture dish field, concretely relates to technical field is primary culture dish of mesenchymal stem cell. The primary culture dish of mesenchymal stem cells comprises a dish cover, a dish bottom and a sample holder. The utility model discloses can solve free mesenchymal stem cell in the longer primary culture time and organize the piece to fuse difficult separation closely, it is not hard up that the liquid process of trading leads to organizing the piece, is unfavorable for mesenchymal stem cell's free problem.
Description
Technical Field
The utility model relates to a culture dish field, concretely relates to technical field is primary culture dish of mesenchymal stem cell.
Background
Mesenchymal stem cells are important members of a stem cell family, and the placenta and the umbilical cord of a pregnant woman after delivery are rich in the mesenchymal stem cells. With the development of stem cell cryopreservation technology, more and more parturients choose to cryopreserve stem cells in the placenta and umbilical cord. Most of the existing separation technologies are primary culture in a culture bottle after tissue cutting, and digestion with digestive enzymes is carried out after mesenchymal stem cells are dissociated to a culture container for adherent growth. On one hand, the culture process usually needs 14-18 days, the mesenchymal stem cells free in a longer primary culture time are fused with the tissue block closely and are not easy to separate, the digested cells need to be filtered to obtain the mesenchymal stem cells, and the operation steps are complicated; on the other hand, the liquid changing process sometimes causes the tissue block to loosen, which is not beneficial to the dissociation of the mesenchymal stem cells.
Disclosure of Invention
The utility model provides a primary culture dish of mesenchymal stem cell, above-mentioned technical problem of solution that can be fine realizes high-efficient separation mesenchymal stem cell.
The utility model provides a technical scheme as follows: the primary culture dish of mesenchymal stem cells comprises a dish cover, a dish bottom and a sample holder.
The dish cover is cylindrical, and the specification of the dish cover is pi × (4-5 cm)2× (1-1.5 cm), the top of the capsule cover of the capsule is provided with 1 row or a plurality of clamping grooves, the number of the clamping grooves is 5-10, and the clamping grooves are used for fixing the sample holder.
The dish bottom is cylindrical, and the specification of the dish bottom is pi × (3.9-4.9 cm)2× (2-2.5 cm), scale marks are arranged at the bottom of the dish.
The sample holder comprises a clamping holder, a sample inlet and a free hole, the sample holder is a cylindrical or other regular cylinder, and the specification of the sample holder is pi × 0.75cm2× (1.7-2.2 cm), the sample support card holder is occluded with the vessel cover clamping groove, the free holes are circular or other regular patterns, the number of the free holes is 2-5, and the specification of the free holes is pi × (0.2-0.3 cm)2. The free holes are regularly distributed on the lower half part of the sample holder.
The beneficial effects of the utility model are that, use the utility model discloses the time does not direct contact through the sample holds in the palm the mesenchymal stem cell that realizes tissue and dissociation, enlarges separation efficiency. Cutting tissue piece samples to 1cm3The size is that a tissue block sample is put in through the sample holder inlet, the sample holder card holder is occluded and fixed with the dish cover clamping groove, and the sample holder is 1-3mm away from the dish bottom due to the height difference between the dish cover and the dish bottomIn direct contact. During the culture process, the mesenchymal stem cells are dissociated out, are dissociated into a culture dish outside the sample holder through the dissociating holes, and grow adherent. When the liquid is changed, the tissue block and the free cells are respectively arranged at the bottom of the dish cover sample support and the dish, so that good physical separation is realized. When the mesenchymal stem cells are digested and collected, the tissue blocks and the free cells are respectively arranged at the bottom of the dish cover sample holder and the bottom of the dish, and the mesenchymal stem cells can be obtained by digesting the cells at the bottom of the dish.
Drawings
Fig. 1 is a schematic diagram of a mesenchymal stem cell primary culture dish structure;
fig. 2 is a schematic diagram of a sample holder structure.
Description of reference numerals: a clamping groove 11, a dish cover 12, a sample holder 13, a dish bottom 14 and scale marks 15; a cartridge 131, a sample inlet 132, and a free well 133.
Detailed Description
The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.
Example 1
① sample is added into the sample holder 13 through the sample inlet 132, ② sample holder 13 is fixed on the card slot 11 by the card holder 131, ③ is covered on the dish cover 12, the sample is immersed by the culture solution and the distance between the sample holder 13 and the dish bottom 14 is 1-3mm through the scale mark 15, ④ mesenchymal stem cells are dissociated from the sample holder 13 to the dish bottom 14 through the dissociating hole 133.
Example 2
The whole operation is carried out in a sterile environment.
① cutting umbilical cord to 1cm in biological safety cabinet3The method comprises the steps of measuring, ②, placing an umbilical cord block into a sample holder 13, ③, installing the sample holder 13 with the umbilical cord block, fixing the sample holder 13 in a clamping groove 11, ④, adding a mesenchymal stem cell separation culture medium, ⑤, covering a dish cover 12, observing the umbilical cord block in the sample holder 13 by using a scale mark 15, observing the umbilical cord block in the sample holder 13 by using the scale mark 15, observing the distance between the sample holder 13 and a dish bottom 14 by using the scale mark 15 to be 1-3mm, ⑥, changing liquid on time, culturing and observing the separation condition of the mesenchymal stem cells for 14-18 days, and ⑦, uncovering the dish cover 12, digesting the mesenchymal stem cells in the dish bottom 14.
Example 3
The whole operation is carried out in a sterile environment.
① cutting placenta to 1cm in a biological safety cabinet3The method comprises the steps of measuring the size of a dish, ② placing a placenta block into a sample holder 13, ③ fixing the sample holder 13 with the placenta block in a clamping groove 11, ④ adding a mesenchymal stem cell separation culture medium, ⑤ covering a dish cover 12, observing the placenta block in the sample holder 13 by a scale mark 15, observing the distance between the sample holder 13 and a dish bottom 14 by the scale mark 15 to be 1-3mm, ⑥ changing liquid on time to culture and observe the separation condition of the mesenchymal stem cells for 14-18 days, and ⑦ uncovering the dish cover 12, digesting the mesenchymal stem cells in the dish bottom 14 and collecting the mesenchymal stem cells.
Claims (1)
1. Mesenchymal stem cell primary culture dish, its characterized in that holds in the palm at the bottom of ware lid, ware, the ware caping of ware lid sets up 1 row or a plurality of draw-in groove, draw-in groove quantity is 5 ~ 10, the draw-in groove is used for fixed sample to hold in the palm, be equipped with the scale mark at the bottom of the ware, the scale mark is used for observing the culture medium and floods the sample in holding in the palm, holds in the palm and holds in the palm the interval at the bottom of the ware by the scale mark observation sample, the sample holds in the palm including the card holds in the palm, sample entry, free hole, the card holds in the palm and is fixed in the draw-in groove, its the free hole is regular figure, is.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920857399.3U CN210945622U (en) | 2019-06-10 | 2019-06-10 | Primary culture dish of mesenchymal stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920857399.3U CN210945622U (en) | 2019-06-10 | 2019-06-10 | Primary culture dish of mesenchymal stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210945622U true CN210945622U (en) | 2020-07-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920857399.3U Active CN210945622U (en) | 2019-06-10 | 2019-06-10 | Primary culture dish of mesenchymal stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210945622U (en) |
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2019
- 2019-06-10 CN CN201920857399.3U patent/CN210945622U/en active Active
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