CN114196541A - Culture plate with filtering function suitable for automatic pipetting system - Google Patents

Culture plate with filtering function suitable for automatic pipetting system Download PDF

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Publication number
CN114196541A
CN114196541A CN202111601795.8A CN202111601795A CN114196541A CN 114196541 A CN114196541 A CN 114196541A CN 202111601795 A CN202111601795 A CN 202111601795A CN 114196541 A CN114196541 A CN 114196541A
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Prior art keywords
culture
plate
hole
culture plate
pipetting system
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CN202111601795.8A
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邢华杨
游明辉
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Hangzhou Aiming Medical Technology Co ltd
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Hangzhou Aiming Medical Technology Co ltd
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Priority to CN202111601795.8A priority Critical patent/CN114196541A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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Abstract

The invention discloses a culture plate with a filtering function and suitable for an automatic pipetting system, which comprises a main plate and a transparent bottom plate, wherein the main plate is fixedly connected with the transparent bottom plate, a plurality of groups of combined holes are arranged in the main plate, the combined holes comprise culture holes and liquid suction holes, and communication channels are arranged at the bottoms of the culture holes and the liquid suction holes. The culture plate can effectively separate suspension cell balls from cell culture solution, and solves the problems of high working strength and complex operation existing in the manual operation of the cell culture plate in the existing organoid automatic culture and drug sensitive screening processes.

Description

Culture plate with filtering function suitable for automatic pipetting system
Technical Field
The invention belongs to the technical field of culture plates, and particularly relates to a culture plate with a filtering function, which is suitable for an automatic pipetting system.
Background
When a cell culture plate is used for organoid and 3D cell ball culture or drug screening, culture waste liquid in culture holes needs to be repeatedly sucked and discarded, and a sample is transferred to a clean and sterile objective table with good light transmittance for imaging identification analysis. And after the identification is finished, transferring the sample back to the culture hole again, and continuing to culture, thereby repeating the steps. If more sample screening state information in the middle section of the culture needs to be obtained, the process is repeated, and the process is discarded, namely plate rotating, plate rotating and discarding. The requirement of screening samples on a large scale and a single and repetitive process increase the working error rate of operators, and the frequent transfer of the samples brings the risk of sample pollution. The automatic liquid changing system greatly accelerates the drug sensitivity test efficiency based on cell culture, but for cells growing in suspension, such as organoid balls, 3D cell balls and the like, the cell balls are mixed with a culture medium, so that the automatic liquid transferring system cannot be used. How to separate the suspension cell balls from the cell culture medium is a key technical difficulty for realizing automatic pipetting.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides the culture plate with the filtering function, which is suitable for an automatic pipetting system, and can effectively solve the problems of high working strength and complex operation of the conventional culture plate.
In order to achieve the purpose, the technical scheme adopted by the invention for solving the technical problems is as follows:
the utility model provides a culture plate with filtering capability suitable for automatic liquid-transfering system, includes mainboard and transparent bottom plate, mainboard and transparent bottom plate fixed connection are provided with a plurality of groups of combination holes in the mainboard, and the combination hole is provided with the intercommunication passageway including cultivateing hole and imbibition hole, cultivation hole and imbibition hole bottom.
In the scheme, the main board and the transparent bottom board are fixed through a welding technology, during cultivation, a sample is added into the cultivation hole, then culture solution is continuously added into the cultivation hole, the culture solution flows into the liquid suction hole through the communication channel, so that the liquid level height in the liquid suction hole is consistent with that in the cultivation hole, after cultivation is finished, the liquid transfer head is inserted into the liquid suction hole for liquid suction, the culture solution in the cultivation hole can be sucked out through the liquid suction hole due to the fact that the cultivation hole is communicated with the liquid suction hole, the required sample can be filtered and retained in the cultivation hole due to the fact that the thickness of the communication channel is small, and the sample cannot block the liquid transfer gun head under the filtering effect of the communication channel; after imbibition is finished, the culture plate is directly placed on an imaging platform for identification and identification.
Furthermore, the liquid suction hole is arranged on one side of the culture hole, and the volume of the liquid suction hole is smaller than that of the culture hole.
Further, two adjacent combination holes are arranged alternately.
In the scheme, two liquid suction holes in two adjacent groups of combined holes are arranged up and down in each transverse row, and two culture holes are respectively arranged on two sides of the two liquid suction holes; the design ensures that the culture plate can realize the filtering function under the condition of keeping the standard specification, thereby improving the use convenience.
Furthermore, the main board is provided with a mark part which is arranged between the culture hole and the liquid suction hole which are communicated with each other.
Further, the identification part is in a convex or concave groove structure.
Among the above-mentioned scheme, set up sign portion between imbibition hole and the cultivation hole that communicates each other, can be convenient for the operator directly perceivedly look over the intercommunicating relation in imbibition hole and cultivation hole, improve the convenience of operation. The mark part is arranged to be of a convex or groove structure, so that the mark part can be directly manufactured in the injection molding process of the main board, and the subsequent operation process is reduced.
Furthermore, an intercepting device is arranged in the culture hole or the communicating channel, and when the intercepting device is arranged in the culture hole, the intercepting device is arranged on one side close to the communicating channel.
Further, the intercepting device comprises at least one upright post.
Among the above-mentioned scheme, intercepting device also makes at mainboard injection moulding in-process, and intercepting device and mainboard integrated into one piece are provided with intercepting device, can be at the imbibition in-process, intercept the sample of cultivateing, avoid the sample to block up the intercommunication passageway, influence the imbibition effect, and the shape of stand can be for toper, cylindrical, cuboid etc.
Further, the culture plate is a 384 culture plate.
Further, the culture well volume was 66. mu.L, the pipette well volume was 30. mu.L, and the thickness of the communicating channel was 0.05 mm.
In the above scheme, because the 384-pore plate has more culture pores, the structure in the application is arranged, the operation convenience can be greatly improved, and the labor amount and the labor intensity of an operator are reduced.
Further, the culture plate with the filtering function is applied to 3D cell culture, organoid culture, suspension cell tissue mass culture or drug screening.
The beneficial effects produced by the invention are as follows:
the culture plate has a filtering function on the basis of meeting the requirement of sample culture, can realize quick suction and disposal of culture solution, and the sample cannot block a suction head. Meanwhile, due to the good light transmission performance of the plate bottom, the culture plate can be directly placed in an imaging area, so that the requirement of imaging identification of a sample is met, the problem of low screening efficiency caused by transfer is solved, and the device can provide more efficient technical support for large-scale screening and the like.
Drawings
FIG. 1 is a schematic top view of the present invention;
FIG. 2 is a schematic bottom view of the present invention;
FIG. 3 is an enlarged view of part A of the present invention;
FIG. 4 is a schematic perspective view of the present invention;
FIG. 5 is an enlarged view of the portion B of the present invention;
reference numerals: 1. a main board; 2. a transparent base plate; 3. a culture well; 4. a liquid suction hole; 5. a communication channel; 6. a marking part; 7. and (4) a column.
Detailed Description
The following detailed description of embodiments of the invention refers to the accompanying drawings.
In one embodiment of the present invention, as shown in fig. 1-3, a filter-function culture plate suitable for an automated pipetting system is provided, which is characterized in that the culture plate comprises a main plate 1 and a transparent bottom plate 2, the main plate 1 is fixedly connected with the transparent bottom plate 2, a plurality of groups of combination holes are arranged in the main plate 1, and optimally, two adjacent groups of combination holes are arranged alternately.
The combined hole comprises a culture hole 3 and a liquid suction hole 4, and a communication channel 5 is arranged at the bottom of the culture hole 3 and the bottom of the liquid suction hole 4. Optimally, the liquid suction hole 4 is arranged at one side of the culture hole 3, and the volume of the liquid suction hole 4 is smaller than that of the culture hole 3. Optimally, the main board 1 is provided with a mark part 6, and the mark part 6 is arranged between the culture hole 3 and the liquid suction hole 4 which are communicated with each other. Preferably, the marking 6 is a protrusion or a groove structure. Optimally, an intercepting device is arranged in the culture hole 3 or the communicating channel 5 and is arranged at one side close to the communicating channel 5. Preferably, the intercepting means comprise at least one upright 7. Optimally, the culture plates are 384 culture plates. Optimally, the culture well 3 has a volume of 66. mu.L and the pipette well 4 has a volume of 30. mu.L. Optimally, the thickness of the communication channel 5 is 0.05 mm.
The use process of the device is as follows: adopt current culture plate to carry out the culture in-process to 3D cell, need relapse abandon the culture waste liquid clean to shift the sample to the aseptic objective table that the light transmissivity is good and carry out the formation of image differentiation, after the differentiation finishes, need shift back again and continue to cultivate in the culture dish, with this repetition, this operation is not only wasted time, and increases real operating personnel's work error rate. In addition, during the culture process, the tissue block is suspended in the culture solution, and when the culture solution is sucked, the suspended tissue block easily blocks the liquid-transferring head, which affects the operation. By adopting the culture plate, the tissue block is only required to be added into the culture hole 3, then the culture solution is continuously added into the culture hole 3, and the culture solution flows into the liquid suction hole 4 through the communicating channel 5, so that the liquid level heights in the liquid suction hole 4 and the culture hole 3 are consistent, and the culture can be carried out; after the culture is finished, the pipetting head is inserted into the liquid washing hole 4 for pipetting, the culture hole 3 is communicated with the liquid aspirating hole 4, the culture liquid in the culture hole 3 can be aspirated out through the liquid aspirating hole 4, the required tissue blocks can be filtered and retained in the culture hole due to the small thickness of the communicating channel 5, and the pipetting head cannot be blocked by a sample under the filtering action of the communicating channel 5; meanwhile, the interception device is arranged, so that the sample can be prevented from blocking the communication channel; after the imbibition is accomplished, directly will cultivate the board place on imaging platform discern the appraisal can, can avoid changeing the board operation, improve the convenience and the efficiency of operation.

Claims (10)

1. The utility model provides a culture plate with filtering capability suitable for automatic liquid-transfering system, its characterized in that, includes mainboard (1) and transparent bottom plate (2), mainboard (1) with transparent bottom plate (2) fixed connection, be provided with a plurality of groups combination hole in mainboard (1), the combination hole is including cultivateing hole (3) and imbibition hole (4), it is provided with intercommunication passageway (5) to cultivate hole (3) and imbibition hole (4) bottom.
2. The culture plate with filter function suitable for an automated pipetting system according to claim 1, wherein the pipette well (4) is provided on one side of the culture well (3), and the pipette well (4) has a volume smaller than the volume of the culture well (3).
3. The filter-enabled culture plate suitable for an automated pipetting system according to claim 2, wherein adjacent two sets of the combination wells are alternately disposed.
4. The filter-enabled culture plate suitable for an automated pipetting system according to claim 1, wherein the main plate (1) is provided with an identification part (6), and the identification part (6) is provided between the culture well (3) and the pipette well (4) which are communicated with each other.
5. The plate with a filter function suitable for an automated pipetting system according to claim 4, wherein the identification portion (6) is a protrusion or a groove structure.
6. The filter-enabled culture plate suitable for an automated pipetting system according to claim 1, wherein an intercepting means is provided in the culture well (3) or the communication channel (5).
7. A filter-enabled culture plate for an automated pipetting system according to claim 6, wherein the intercepting means comprises at least one upright (7).
8. The filter-enabled culture plate suitable for use in an automated pipetting system of claim 1, wherein the culture plate is a 384 culture plate.
9. The culture plate with filter function suitable for use in an automated pipetting system according to claim 8, wherein the culture well (3) has a volume of 66 μ L, the pipette well (4) has a volume of 30 μ L, and the communication channel (5) has a thickness of 0.05 mm.
10. Use of the filter-enabled culture plate for an automated pipetting system according to any one of claims 1 to 9 in 3D cell culture, organoid culture, tissue mass culture in suspension cells or drug screening.
CN202111601795.8A 2021-12-24 2021-12-24 Culture plate with filtering function suitable for automatic pipetting system Pending CN114196541A (en)

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CN202111601795.8A CN114196541A (en) 2021-12-24 2021-12-24 Culture plate with filtering function suitable for automatic pipetting system

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Application Number Priority Date Filing Date Title
CN202111601795.8A CN114196541A (en) 2021-12-24 2021-12-24 Culture plate with filtering function suitable for automatic pipetting system

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CN114196541A true CN114196541A (en) 2022-03-18

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4245042A (en) * 1978-01-26 1981-01-13 Yeda Research And Development Co. Ltd. Device for harvesting cell cultures
CN201933086U (en) * 2011-01-04 2011-08-17 南京市胸科医院 Micro-volume testing plate for primarily testing fast drug susceptibility and bacteria types of combination of tuberculosis and anti-acid mycobacterium
CN207608545U (en) * 2017-12-07 2018-07-13 齐齐哈尔医学院 It is a kind of to be directed to the at the uniform velocity controllable imbibition of 24 porocyte culture plates and chemicals dosing plant
CN112042531A (en) * 2019-06-07 2020-12-08 南宁学院 Plant tissue culture rack capable of quickly taking samples
US20210079329A1 (en) * 2017-07-13 2021-03-18 Greiner Bio-One North America, Inc. Culture plates for imaging
CN214032536U (en) * 2020-07-06 2021-08-24 广州华越肾科再生医学科技有限公司 Cell culture plate without damaging matrigel and cells

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4245042A (en) * 1978-01-26 1981-01-13 Yeda Research And Development Co. Ltd. Device for harvesting cell cultures
CN201933086U (en) * 2011-01-04 2011-08-17 南京市胸科医院 Micro-volume testing plate for primarily testing fast drug susceptibility and bacteria types of combination of tuberculosis and anti-acid mycobacterium
US20210079329A1 (en) * 2017-07-13 2021-03-18 Greiner Bio-One North America, Inc. Culture plates for imaging
CN207608545U (en) * 2017-12-07 2018-07-13 齐齐哈尔医学院 It is a kind of to be directed to the at the uniform velocity controllable imbibition of 24 porocyte culture plates and chemicals dosing plant
CN112042531A (en) * 2019-06-07 2020-12-08 南宁学院 Plant tissue culture rack capable of quickly taking samples
CN214032536U (en) * 2020-07-06 2021-08-24 广州华越肾科再生医学科技有限公司 Cell culture plate without damaging matrigel and cells

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