CN201195724Y - Tumour cell drug sensitive test compound checker board - Google Patents
Tumour cell drug sensitive test compound checker board Download PDFInfo
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- CN201195724Y CN201195724Y CNU2008200459510U CN200820045951U CN201195724Y CN 201195724 Y CN201195724 Y CN 201195724Y CN U2008200459510 U CNU2008200459510 U CN U2008200459510U CN 200820045951 U CN200820045951 U CN 200820045951U CN 201195724 Y CN201195724 Y CN 201195724Y
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- checkout
- sensitive test
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Abstract
The utility model discloses a tumor cell drug sensitive test compound checkout console, which comprises a console cover (1) and a checkout console body (3), wherein a plurality of drug sensitive checkout holes (31) are arranged on the checkout console body (3), a drug storage plate (2) is also arranged between the checkout console body and the console cover, drug adding holes (21) which are corresponding to the drug sensitive checkout holes on the checkout console body are arranged on the drug storage plate, drug membrane is arranged on the drug adding holes, and the drug storage plate is covered on the checkout console body. The tumor cell drug sensitive test compound checkout console transfers and arranges the drug membrane of chemotherapy drug to the drug storage plate, tumor cells can naturally grow under the condition that the tumor cells are not affected by drug when in culture, since the drug membrane of the chemotherapy drug is adhered on the drug adding holes of the drug storage plate, the checking means is simplified and standardized, thereby the use of the tumor cell drug sensitive test compound checkout console enables drug sensitive tests to be more convenient, and more objective and more accurate results can be obtained.
Description
Technical field
The utility model relates to the detection technique field, in particular for the cell detection plate of the external drug sensitive test of tumour.
Background technology
In the clinical treatment of tumour, usually adopt " individualized treatment ", one of them important step is the external drug sensitive test of tumour cell, and the external drug sensitive test of tumour cell is that patient's tumour cell is measured antitumor drug susceptibility and chemical sproof a kind of experimental technique in vitro culture and after antitumor drug is handled.This method is avoided the abuse of invalid medicine and reduces toxic side effect that to improving the efficient of chemotherapy huge meaning is arranged.The check-out console that is used as tumor cell culture and drug treating in the test then plays critical effect to the accuracy and the reliability of drug sensitive test.
Existing medicine sensitive detecting plate is to utilize traditional porous cell culture plate to do the detection to the tumour cell effect of tumor cell culture and medicine basically, no matter be laboratory applications or the check-out console on the market in " the tumour drug sensitive test test kit " of supply, its structure formation substantially the same (only changing to some extent aspect what) in the quantity in hole, this class check-out console is formed by detecting plate body and plate lid, detect plate body multirow and the evenly distributed cylindrical vessel of multiple row are set, these containers are called susceptibility and detect the hole, as cell cultures and drug sensitive test container.And the plate lid is to be provided with frame all around by the strip plate, mainly act as the cleaning and the interior humidity of retaining hole that keep plate body.When experiment, tumor cell inoculation adds different antitumor drugs tumour cell is handled in culture hole in each culture hole.Thereby the effect to medicine behind certain hour detects susceptibility and the resistance of judging antitumor drug.Make a general survey of these check-out consoles, two big classes are arranged basically, the first kind is not to be with chemotherapeutics, chemotherapeutics is prepared (requiring with joining with using for anti-medicine changes) voluntarily by operator, owing to generally all must select for use multiple (generally having several) medicine to carry out sensitization test to tens kinds at experimentation, add that every kind of medicine must be mixed with multiple different concns, therefore operation is more complicated, technical requirements height to operator, and the total unavoidable error that produces of manual operation, the reliability of every data of being obtained is subjected to certain limitation by experiment.Second class has chemotherapeutics, this class check-out console is that chemotherapeutics is made earlier through freezing, dry and the quantitative medicine film of solidified is attached to and detects at the bottom of the hole, its major advantage is, simplify procedures, prevent the generation of the human error that above-mentioned first kind check-out console is easy to occur, but it also exists shortcoming: tumour cell is separated from tumor tissues in the vitro detection plate and is cultivated, need the new environment of for some time adaptation and just can reach the self-sow state, such as most of tumour cells need 24 hours the times could adherent growth, also need certain hour ability normal growth after the cell that minority is not adherent such as the leukemia cell's inoculation, in the experimentation of using the second class check-out console, behind the cell and nutrient solution adding for test, the dissolving of medicine film fully just reaches predetermined final concentration, tumour cell does not reach self-sow state (for example attached cell as yet not adherent growth) as yet, just be placed under the effect of chemotherapeutics, chemotherapeutics differs widely to the situation of the effect of tumour cell in this and the body, false-positive result may occur, therefore the reliability of the experimental result that is obtained is restricted equally.The existing structure of visible detection plate has restricted drug sensitive test result's accuracy, the further raising of reliability significantly.
Summary of the invention
The purpose of this utility model provides a kind of drug sensitive test of tumor cell and tests compound check-out console, both can prepare in advance that thereby the patent medicine film prevents from manually to make up a prescription as commercial standard detection plate and the generation of application of sample error with detecting medicine commonly used, again can tumour cell reach self-sow state (for example tumour cell adherent growth) thus in add the generation that medicine is avoided false positive results.
For achieving the above object, the technical solution of the utility model is: a kind of drug sensitive test of tumor cell is tested compound check-out console, comprise and detect plate body and Ban Gai, detect a plurality of susceptibility detections hole is set on the plate body, also have a storage medicine plate between plate body and the plate lid detecting, storage medicine plate be provided with check-out console on susceptibility detect corresponding dosing hole, hole, the dosing hole is provided with the medicine film, storage medicine plate covers on check-out console, and plate covers on storage medicine plate.
Dosing hole on the storage medicine plate is a funnel-form, and the little spoon that holds the medicine film is arranged in the middle of its bottom.
Dosing hole on the storage medicine plate is the soupspoon shape.
Be provided with the correction frame between storage medicine plate and the detection plate body.
The utility model three plates unification when storing become one box-like, require to deposit in and be not damaged with protection medicine film in the refrigerator or pollute.Operation is as follows when experimentizing: take out and detect plate body (storage medicine plate is still deposited in the refrigerator), with separate and cell count the tumour cell of accurate measurement and nutrient solution thereof add the susceptibility that detects plate body and detect in the hole, putting into incubator routinely cultivated 24 hours, make adherent the reaching of tumour cell similar in appearance to the self-sow state for test, can add storage medicine plate this moment by correction position, making funnel on the storage medicine plate or the medicine in the soupspoon be dipped in susceptibility detects in the nutrient solution in hole and dissolving, the concentration that detects the hole Chinese traditional medicine meets design concentration (being prescribed concentration in the product description) exactly, after in incubator, cultivating 2-7 days as required again, measure tumour cell survival and apoptosis (can adopt the whole bag of tricks such as comprising bioluminescence and mtt assay), relevant data is calculated, mapping is again according to the definite medicine to the tumour cell sensitivity of existing standard.
The beneficial effects of the utility model are, it is arranged on the medicine film of chemotherapeutics on the storage medicine plate by the check-out console transfer, like this when cultivating, tumour cell can be under the situation that is not subjected to drug influence self-sow, be attached on the dosing hole of storing the medicine plate owing to quantitative medicine film is made through freezing, dry and curing by chemotherapeutics elder generation again, made detection means simplification and stdn, therefore use of the present utility model, make drug sensitive test more convenient, and have more objective, result more accurately.
Description of drawings
Fig. 1 is the front view of the utility model embodiment 1;
Fig. 2-the 1st, the front view of plate lid among the embodiment 1;
Fig. 2-the 2nd, the vertical view of plate lid among the embodiment 1;
Fig. 3-the 1st, the front view of storage medicine plate among the embodiment 1;
Fig. 3-the 2nd, the vertical view of storage medicine plate among the embodiment 1;
Fig. 3-the 3rd, the synoptic diagram in dosing hole among the embodiment 1;
Fig. 4-the 1st, the front view of detection plate body among the embodiment 1;
Fig. 4-the 2nd, the vertical view of detection plate body among the embodiment 1;
Fig. 5 is the front view of the utility model embodiment 2;
Fig. 6-the 1st, the front view of storage medicine plate among the embodiment 2;
Fig. 6-the 2nd, the vertical view of storage medicine plate among the embodiment 2;
Fig. 6-the 3rd, the synoptic diagram in dosing hole among the embodiment 2;
Fig. 7 is the utility model assembled state synoptic diagram.
Embodiment
Embodiment 1:
Shown in Fig. 1～Fig. 4-2, check-out console is divided into three parts, and Fig. 1 is an overview, and check-out console comprises plate lid 1, storage medicine plate 2 and detects plate body 3, made by the material that is fit to the ordinary cells cultivation.Fig. 2 is a plate lid view, and the plate lid is mainly used in the protection check-out console and is not subjected to external environmental and the interior humidity of retaining hole.Fig. 4 is for detecting the view of plate body, and it is identical with general detection plate body to detect plate body, has row, equally distributed 24,96,384 susceptibility of row to detect the plate in hole 31; This explanation is an example with 24 orifice plates, and that detects plate body also has and the corresponding correction frame of storage medicine plate all around.Fig. 3 is storage medicine plate, on the storage medicine plate funnelform dosing hole 21 is arranged, the position in dosing hole is corresponding with the susceptibility detection hole on detecting plate body, the little spoon 22 that holds the medicine film is arranged in the middle of the bottom of funnel, be added with quantitative chemotherapeutics medicine film in the little spoon, this medicine film has passed through vacuum, drying, curing and fungi-proofing processing, should store in the refrigerator.Storage medicine plate is when detecting plate body and be harmonious, and susceptibility detects the hole and coincide with funnelform dosing hole, because correction is arranged, so not only hole and hole coincide, and its order arranges by design fully, and direction will not be opposite when assurance was harmonious.During storage three plates unifications become one box-like, require to deposit in and be not damaged with protection medicine film in the refrigerator or pollute.
Embodiment 2:
Similar to Example 1, be the structure difference of dosing plate.Shown in Fig. 5～Fig. 6-3, the dosing hole 21 on the storage medicine plate 2 is a soupspoon shape, holds the medicine film in the soupspoon.
Claims (4)
1. a drug sensitive test of tumor cell is tested compound check-out console, comprise plate lid (1) and detect plate body (3), detect a plurality of susceptibility detections hole (31) is set on the plate body (3), it is characterized in that: also have a storage medicine plate (2) between described detection plate body (3) He Bangai (1), storage medicine plate (2) is provided with and detects plate body (3) and goes up susceptibility detection corresponding dosing hole, hole (31) (21), dosing hole (21) is provided with the medicine film, storage medicine plate (2) covers and is detecting on the plate body (3), and plate lid (1) covers on storage medicine plate (2).
2. drug sensitive test of tumor cell according to claim 1 is tested compound check-out console, it is characterized in that: the dosing hole (21) on the described storage medicine plate (2) is a funnel-form, and the little spoon (22) that holds the medicine film is arranged in the middle of the bottom of dosing hole (21).
3. drug sensitive test of tumor cell according to claim 1 is tested compound check-out console, it is characterized in that: the dosing hole (21) on the described storage medicine plate (2) is the soupspoon shape.
4. test compound check-out console according to each described drug sensitive test of tumor cell of claim 1 to 3, it is characterized in that: be provided with the correction frame between described storage medicine plate (2) and the detection plate body (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200459510U CN201195724Y (en) | 2008-04-03 | 2008-04-03 | Tumour cell drug sensitive test compound checker board |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200459510U CN201195724Y (en) | 2008-04-03 | 2008-04-03 | Tumour cell drug sensitive test compound checker board |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201195724Y true CN201195724Y (en) | 2009-02-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008200459510U Expired - Fee Related CN201195724Y (en) | 2008-04-03 | 2008-04-03 | Tumour cell drug sensitive test compound checker board |
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| Country | Link |
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| CN (1) | CN201195724Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103088102A (en) * | 2013-01-09 | 2013-05-08 | 苏州麦克威尔生物医药科技有限公司 | Sterile instant type medicine film and tumor drug sensitivity testing application thereof |
| CN106248922A (en) * | 2016-07-22 | 2016-12-21 | 嘉兴鼎诺生物科技有限公司 | A kind of drug sensitive test of tumor cell detection plate and detection method |
-
2008
- 2008-04-03 CN CNU2008200459510U patent/CN201195724Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103088102A (en) * | 2013-01-09 | 2013-05-08 | 苏州麦克威尔生物医药科技有限公司 | Sterile instant type medicine film and tumor drug sensitivity testing application thereof |
| CN103088102B (en) * | 2013-01-09 | 2015-05-20 | 苏州麦克威尔生物医药科技有限公司 | Sterile instant type medicine film and tumor drug sensitivity testing application thereof |
| EP2944698B1 (en) * | 2013-01-09 | 2018-10-03 | Suzhou Macwell Biomedical Sci & Tech Co. Ltd | Sterile and instantly dissolvable drug membrane and use thereof for testing tumor drug sensitivity |
| CN106248922A (en) * | 2016-07-22 | 2016-12-21 | 嘉兴鼎诺生物科技有限公司 | A kind of drug sensitive test of tumor cell detection plate and detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090218 Termination date: 20160403 |