CN105519494B - A kind of experiment supports seedling device and its test method with foster worm - Google Patents

A kind of experiment supports seedling device and its test method with foster worm Download PDF

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Publication number
CN105519494B
CN105519494B CN201610058033.0A CN201610058033A CN105519494B CN 105519494 B CN105519494 B CN 105519494B CN 201610058033 A CN201610058033 A CN 201610058033A CN 105519494 B CN105519494 B CN 105519494B
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honeydew
supported
seedling
worm
chassis
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CN105519494A (en
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王爱英
唐健
刘淑华
杨保军
罗举
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China National Rice Research Institute
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China National Rice Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A kind of experiment supports seedling device and its test method with foster worm, belongs to insect experimental rig and test method technical field.Including chassis, seedling cup, bucket cover and honeydew measurement bag are supported, chassis is opened upper end structure, and chassis side wall top sets hanging hole, and tray bottom sets discharge outlet, in discharge outlet plastic plug set to seal;Iron wire both ends pass through hanging hole by chassis split into grid space;Fixed placement supports seedling cup in each grid, supports and a notch is at least set at the top of seedling cup, supports the stalk of planting plants or blade in seedling cup and is placed on gap portions;Insect to be measured is placed in honeydew measurement bag, gathers on honeydew measurement bag and aperture is set, honeydew measurement bag is fixed on the plant haulm or blade for being placed on gap portions during use;Seedling cup top cooperation bucket cover is supported, screen window lid is equipped at the top of bucket cover.A kind of above-mentioned experiment supports seedling device and its test method with foster worm, and the apparatus structure is simple, easy to make, highly practical.

Description

A kind of experiment supports seedling device and its test method with foster worm
Technical field
The invention belongs to insect experimental rig and test method technical fields, are specially that a kind of experiment supports seedling device with foster worm And its test method.
Background technology
Plant resources and insect resources are the huge resources banks of the mankind, in recent years biological medicine, food, health products, The fields such as feed and chemical industry play huge effect, further to excavate plant and the insect one side beneficial to the mankind, avoid Harm caused by it is, it is necessary to carry out molecular biology, the research of ecology waits etc..Plant and insect carry out molecular biology During the research of aspect, material utilization amount is few, but requirement cannot finely have other interference, other experimental studies are also required to artificially control Plant processed and the environment or influence factor of insect growth development.Existing a variety of foster seedlings support the method for worm, and there are various Defect most importantly can not effectively avoid external environment to the interference for examination plant and insect and artificial control insect and plant The inside and outside environment of growth.Nowadays extensively using the measure of honeydew among the ecological study of the hemipterans such as plant hopper As the indirect method for obtaining test worm food ingestion, most important problem is to connect worm difficulty in the assay method of honeydew, and test worm is not allowed The easily feeding on ready plant.Food materials of the more sturdy plant of forefathers' more options as test worm, material are not easy to cultivate, and increase Add workload, and easily cause test worm escape.
The content of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to design a kind of experiment of offer to be supported with foster worm The technical solution of seedling device and its test method, it is simple in structure, it is at low cost, it is easy to use.
A kind of experiment supports seedling device with foster worm, it is characterised in that including chassis, supports seedling cup, bucket cover and honeydew measurement Bag, chassis are opened upper end structure, and chassis side wall top sets hanging hole, and tray bottom sets discharge outlet, modeling is set in discharge outlet Rubber plug seals;Iron wire both ends pass through hanging hole by chassis split into grid space;Fixed placement supports seedling cup in each grid, supports seedling cup Top at least sets a notch, supports the stalk of planting plants or blade in seedling cup and is placed on gap portions;In honeydew measurement bag It places insect to be measured, gathers on honeydew measurement bag and aperture is set, honeydew measurement bag, which is fixed on, during use is placed on gap portions On plant haulm or blade;Seedling cup top cooperation bucket cover is supported, screen window lid is equipped at the top of bucket cover.
A kind of experiment supports seedling device with foster worm, it is characterised in that supports and 2-6 notch is set at the top of seedling cup.
A kind of experiment supports seedling device with foster worm, it is characterised in that supports seedling bottom of a cup portion and sets through hole.
A kind of experiment supports seedling device with foster worm, it is characterised in that and it supports seedling cup top inner wall and is threaded, bucket cover bottom Corresponding screw thread, supports seedling cup, bucket cover threaded inside portion.
A kind of experiment supports seedling device with foster worm, it is characterised in that tear line is set on honeydew measurement bag, honeydew is surveyed The opening for determining bag sets a lengthy motion picture, a short-movie, and a long on piece sets adhesive sticker.
The test method of seedling device is supported in a kind of experiment with worm is supported, it is characterised in that is comprised the following steps:
1)It will each support and culture medium planting plants are used in seedling cup, then iron wire both ends are passed through into hanging hole by chassis split into grid Nutrient solution is injected in space in chassis;The foster seedling cup of fixed placement one in each grid, foster Miao Beishang are equipped with bucket cover, bucket cover Top is equipped with screen window lid;It supports and a notch is at least set at the top of seedling cup, support the stalk or blade of planting plants in seedling cup It is placed on gap portions;In use, placing insect to be measured in honeydew measurement bag, gathering on honeydew measurement bag sets aperture, honeydew Bag is measured to be fixed in the stalk or blade of plant;Experiment is supported the assembling of seedling device with foster worm and is finished;
2)It assembled experiment with foster worm is supported into seedling device is put into incubator and cultivate, required every test is set to join Number tears honeydew measurement bag after the test, takes out the insect to be measured in honeydew measurement bag, honeydew measurement is carried out with electronic balance Or research insect life table.
The test method of seedling device is supported in a kind of experiment with worm is supported, it is characterised in that step 2)In:It is set in incubator Put cultivation temperature, light application time and CO2Concentration, research cultivation temperature, light application time and CO2Concentration sends out plant and insect growth The influence educated.
The test method of seedling device is supported in a kind of experiment with worm is supported, it is characterised in that step 2)In:In chassis Heavy metal or trace element are added in nutrient solution, studies its influence to plant or insect.
A kind of above-mentioned experiment supports seedling device and its test method with foster worm, which is suitable for the insects such as planthopper, aphid Raising and research, suitable for the cultivation and research of the crops such as rice, corn, wheat.This is supported worm and supports seedling device using plastics It is made, it is cost-effective, can be disposable, reduce cross contamination;This is supported worm and supports the big of the foster seedling cup of seedling device, chassis and bucket cover Small, highly, often disk quantity etc. can need be adjusted according to experiment, can industrialize, standardize, seriation manufacture, structure Simply, it is easy to make, it is highly practical.
Description of the drawings
Fig. 1 is the chassis structure schematic diagram of the present invention;
Fig. 2 is the iron wire structure diagram of the present invention;
Fig. 3 is the foster seedling cup structure schematic diagram one of the present invention;
Fig. 4 is the foster seedling cup structure schematic diagram two of the present invention;
Fig. 5 is the bucket cover structure schematic diagram of the present invention;
Fig. 6 is the honeydew measurement bag structure diagram of the present invention;
In figure:1- chassis, 101- hanging holes, 102- discharge outlet, 103- plastic plugs, 2- iron wires, 3- support seedling cup, 301- notches, 302- through holes, 4- barrels of covers, 401- screen windows lid, 5- honeydew measurements bag, 501- apertures, 502- tear lines, 503- adhesive stickers, 504- Lengthy motion picture, 505- short-movies.
Specific embodiment
Below in conjunction with Figure of description, the invention will be further described.
As shown in the figure, the experiment supports seedling device with foster worm, including chassis 1, seedling cup 3, bucket cover 4 and honeydew measurement bag 5, bottom are supported Disk 1 is opened upper end structure, and 1 side wall upper part of chassis sets hanging hole 101, and 1 bottom of chassis sets discharge outlet 102, in discharge outlet 102 Plastic plug 103 is set to seal;2 both ends of iron wire pass through hanging hole 101 by 1 split into grid space of chassis;Fixation is put in each grid Foster seedling cup 3 is put, 3 top of seedling cup is supported and one notch 301 is at least set, support the stalk of planting plants or blade in seedling cup 3 and be placed on 301 position of notch;Insect to be measured is placed in honeydew measurement bag 5, gathers on honeydew measurement bag 5 and aperture 501 is set, honeydew during use Measure bag 5, which is fixed on, to be placed on the plant haulm or blade at 301 position of notch;3 top cooperation bucket cover 4 of seedling cup is supported, bucket cover 4 pushes up Portion is equipped with screen window lid 401.It supports 3 top of seedling cup and 2-6 notch 301 is set.It supports 3 bottom of seedling cup and through hole 302 is set.Support seedling 3 top inner walls of cup are threaded, and 4 bottom internal of bucket cover corresponds to screw thread, foster seedling cup 3,4 threaded of bucket cover, during available for supporting worm Prevent the cross contamination between insect escape and different insects strain, bucket cover 4 is transparent material, and the screen window lid 401 at top can Ventilative light transmission does not interfere with plant and insect growth development.Each foster seedling cup 3 has independent bucket cover 4, can one cuvette of a cuvette Insect is raised, is conveniently replaceable no worm seedling, and fluke is convenient.Tear line 502 is set on honeydew measurement bag 5, and honeydew measurement bag 5 is opened One lengthy motion picture 504, a short-movie 505 at mouthful are set, adhesive sticker 503 is set on a lengthy motion picture 504.Fixed placement supports seedling cup in each grid 3, it is ensured that support the steady of seedling cup 3;The discharge outlet 102 of 1 bottom of chassis has plastic plug 103 to seal when flat, can before replacement nutrient solution It extracts plastic plug 103 and discharges nutrient solution extra in chassis 1.This is supported worm and supports there are two types of the foster seedling cups 3 of seedling device, and a kind of bottom has Hole, a kind of bottom is non-porous, can add in matrix in the porose foster seedling cup in bottom, nutrition is drawn from chassis 1 by the hole of bottom Liquid ensures the growth of culture plant;Medium culture plant can be added in the non-porous foster seedling cup 3 in bottom.Honeydew measurement bag 5 is opened One lengthy motion picture 504, a short-movie 505 at mouthful are set, adhesive sticker 503 is set on a lengthy motion picture 504.It is preferred that the opening for supporting seedling cup 3 has Four notches 301, an at least planting on culture medium and stretches out culture medium, the stalk or blade of plant during honeydew measurement It is placed at notch 301, insect to be measured will be put into honeydew measurement bag 5(Generally planthopper or aphid etc.), during use behind one Short-movie 505 licks to surround stalk, before a lengthy motion picture 504 lick and cling, honeydew measurement bag 5 is fixed in stalk, and honeydew is surveyed Determine have tear line 502 on bag 5, experiment terminates the insects to be measured such as peelable taking-up brown paddy plant hopper, and honeydew measurement is carried out with electronic balance. It is covered with intensive aperture 501 on honeydew measurement bag 5, can prevents from measuring bag cohesion collection steam interference honeydew measurement.Plant and insect Carry out molecular biology in terms of research when, material utilization amount is few, but requirement cannot finely have other interference, this support worm support seedling device Incubator can be put into, nutrient solution is applicable in or worm is supported in culture medium nursery, it is ensured that environment is consistent, and nutrition is consistent, excludes other interference Factor can obtain satisfactory plant and for examination insect, and convenient follow-up progress genome, proteomics etc. is ground Study carefully.This is supported worm and supports the research that seedling device can also be used for insect life table, measures the hatching rate of insect, egg laying amount, survival rate, adult Service life etc. can also add in heavy metal or trace element in nutrient solution or culture medium, study its influence to plant or insect.It should Foster worm supports seedling device and is conveniently put into progress different temperatures, light application time, CO in incubator2The factors such as concentration give birth to plant and insect The research of the influence of long development etc..This is supported worm and supports raising and research that seedling device is suitable for the insects such as planthopper, aphid, fits For the cultivation and research of the crops such as rice, corn, wheat.The foster worm supports seedling device and can be finished with plastic, cost-effective, can It is disposable, cross contamination is reduced, this is supported worm and supports the foster seedling cup 3 of seedling device, the size on chassis 1 and bucket cover 4, often height, disk Quantity etc. can need to be adjusted according to experiment, can industrialize, standardize, seriation manufacture, simple in structure, making side Just, it is highly practical.
The test method of seedling device is supported with foster worm using the experiment, is comprised the following steps:
1)It will each support and culture medium planting plants are used in seedling cup 3, then chassis 1 will be split through hanging hole 101 in 2 both ends of iron wire Into mesh space, nutrient solution is injected in chassis 1;The foster seedling cup 3 of fixed placement one in each grid, supports and is equipped on seedling cup 3 Bucket cover 4,4 top of bucket cover are equipped with screen window lid 401;It supports 3 top of seedling cup and one notch 301 is at least set, support interior kind of seedling cup 3 The stalk or blade for planting plant are placed on 301 position of notch;In use, insect to be measured, honeydew measurement are placed in honeydew measurement bag 5 It gathers on bag 5 and aperture 501 is set, honeydew measurement bag 5 is fixed in the stalk or blade of plant;Experiment supports seedling device group with foster worm It installs complete;
2)It assembled experiment with foster worm is supported into seedling device is put into incubator and cultivate, required every test is set to join Number tears honeydew measurement bag 5 after the test, takes out the insect to be measured in honeydew measurement bag 5, honeydew survey is carried out with electronic balance Fixed or research insect life table.
Above-mentioned steps 2)In:Cultivation temperature, light application time and CO2 concentration, research cultivation temperature, illumination are set in incubator The influence that time and CO2 concentration develop plant and insect growth.
Above-mentioned steps 2)In:Heavy metal or trace element are added in nutrient solution in chassis 1, studies it to plant or elder brother The influence of worm.

Claims (7)

1. a kind of test the test method that seedling device is supported with foster worm, it is characterised in that seedling device is supported using foster worm and is tested, it should Device includes chassis(1), support seedling cup(3), bucket cover(4)With honeydew measurement bag(5), chassis(1)For opened upper end structure, chassis (1)Side wall upper part sets hanging hole(101), chassis(1)Bottom sets discharge outlet(102), discharge outlet(102)Interior setting plastic plug (103)Sealing;Iron wire(2)Both ends pass through hanging hole(101)By chassis(1)Split into grid space;Fixed placement in each grid Support seedling cup(3), support seedling cup(3)Top at least sets a notch(301), support seedling cup(3)The stalk or blade of interior planting plants It is placed on notch(301)Position;Honeydew measurement bag(5)It is interior to place insect to be measured, honeydew measurement bag(5)Upper gather sets aperture (501), honeydew measurement bag during use(5)It is fixed on and is placed on notch(301)On the plant haulm or blade at position;Support seedling cup (3)Top coordinates bucket cover(4), bucket cover(4)Top is equipped with screen window lid(401);
Method comprises the following steps:
1)Seedling cup will each be supported(3)It is interior to use culture medium planting plants, then by iron wire(2)Both ends pass through hanging hole(101)By chassis(1) Split into grid space, chassis(1)Interior injection nutrient solution;The foster seedling cup of fixed placement one in each grid(3), support seedling cup(3) On be equipped with bucket cover(4), bucket cover(4)Top is equipped with screen window lid(401);Support seedling cup(3)Top at least sets one Notch(301), support seedling cup(3)The stalk or blade of interior planting plants are placed on notch(301)Position;In use, honeydew measurement Bag(5)It is interior to place insect to be measured, honeydew measurement bag(5)Upper gather sets aperture(501), honeydew measurement bag(5)It is fixed on plant Stalk or blade on;Experiment is supported the assembling of seedling device with foster worm and is finished;
2)It assembled experiment with foster worm is supported into seedling device is put into incubator and cultivate, required every test parameters is set, Honeydew measurement bag is torn after the test(5), take out honeydew measurement bag(5)Interior insect to be measured carries out honeydew with electronic balance Measure or study insect life table.
2. the test method of seedling device is supported in a kind of experiment as described in claim 1 with worm is supported, it is characterised in that step 2)In:Training It supports and cultivation temperature, light application time and CO is set in case2Concentration, research cultivation temperature, light application time and CO2Concentration is to plant and elder brother The influence of worm growth and development.
3. the test method of seedling device is supported in a kind of experiment as described in claim 1 with worm is supported, it is characterised in that step 2)In: Chassis(1)Heavy metal or trace element are added in interior nutrient solution, studies its influence to plant or insect.
4. the test method of seedling device is supported in a kind of experiment as described in claim 1 with worm is supported, it is characterised in that supports seedling cup(3)Top Portion sets 2-6 notch(301).
5. the test method of seedling device is supported in a kind of experiment as described in claim 1 with worm is supported, it is characterised in that supports seedling cup(3)Bottom Portion sets through hole(302).
6. the test method of seedling device is supported in a kind of experiment as described in claim 1 with worm is supported, it is characterised in that supports seedling cup(3)Top Portion's inner wall is threaded, bucket cover(4)Bottom internal corresponds to screw thread, supports seedling cup(3), bucket cover(4)Threaded.
7. the test method of seedling device is supported in a kind of experiment as described in claim 1 with worm is supported, it is characterised in that honeydew measurement bag (5)Upper setting tear line(502), honeydew measurement bag(5)Opening set a lengthy motion picture(504), a short-movie(505), a lengthy motion picture (504)Upper setting adhesive sticker(503).
CN201610058033.0A 2016-01-28 2016-01-28 A kind of experiment supports seedling device and its test method with foster worm Active CN105519494B (en)

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Publication number Priority date Publication date Assignee Title
CN106614403A (en) * 2016-12-30 2017-05-10 福建省农业科学院水稻研究所 Rice planthopper breeding and observing apparatus and use method of same
CN106993584A (en) * 2017-04-01 2017-08-01 安徽科技学院 A kind of kaoliang aphid life table experimental method
CN108287009A (en) * 2018-04-27 2018-07-17 福建省农业科学院植物保护研究所 A kind of planthopper honeydew amount determining device and its application method

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