CN106993584A - A kind of kaoliang aphid life table experimental method - Google Patents
A kind of kaoliang aphid life table experimental method Download PDFInfo
- Publication number
- CN106993584A CN106993584A CN201710213362.2A CN201710213362A CN106993584A CN 106993584 A CN106993584 A CN 106993584A CN 201710213362 A CN201710213362 A CN 201710213362A CN 106993584 A CN106993584 A CN 106993584A
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- CN
- China
- Prior art keywords
- aphid
- vessel
- kaoliang
- young
- sorghum
- Prior art date
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Links
- 241001124076 Aphididae Species 0.000 title claims abstract description 75
- 244000046109 Sorghum vulgare var. nervosum Species 0.000 title claims abstract description 23
- 238000002474 experimental method Methods 0.000 title claims abstract description 18
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims abstract description 21
- 239000002689 soil Substances 0.000 claims abstract description 18
- 241000209072 Sorghum Species 0.000 claims abstract description 13
- 240000006394 Sorghum bicolor Species 0.000 claims abstract description 11
- 230000004083 survival effect Effects 0.000 claims abstract description 9
- 238000005266 casting Methods 0.000 claims abstract description 8
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 230000000717 retained effect Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 238000004088 simulation Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000015503 Sorghum bicolor subsp. drummondii Nutrition 0.000 description 1
- 240000003829 Sorghum propinquum Species 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 235000006923 Sorghum x drummondii Nutrition 0.000 description 1
- 244000099500 Sudangras Species 0.000 description 1
- 244000152045 Themeda triandra Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000028514 leaf abscission Effects 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
A kind of kaoliang aphid life table experimental method, mainly there is following steps：Soil is added in vessel；A Sorghum Seedlings are cultivated in soil；Virgin paper sheet is covered in soil surface；Female aphid of kaoliang aphid is connect on Sorghum Seedlings, then female aphid is rejected and stays young aphid；Single seedling list aphid carries out life table data record, every time during observation son aphid, the young aphid survival condition of record, cast off a skin time and number of times, and the removal of casting off a skin that young aphid on virgin paper sheet will be fallen, every time during observation adult, aphid survival condition, production aphid quantity are recorded, and aphid that it is farrowed removes discarding, untill aphid death.In the method that the present invention is provided, Sorghum Seedlings can provide the moisture and nutrient of abundance for the kaoliang aphid in experiment, and for accurate simulation kaoliang aphid, the state of free living provides condition on the sorghum plant of field；Aphid bits of casting off a skin fall on virgin paper sheet, for accurate observation, record；Vessel ensure that casting off a skin for kaoliang aphid does not float；Conveniently can intuitively it be observed by window.
Description
Technical field
The present invention relates to insect life table experimental method, specifically a kind of kaoliang aphid life table experimental method.
Background technology
Life table be study insect grow and biological characteristics experimental method, kaoliang aphid be harm sorghum, Soviet Union
The primary pest of red grass and Sorghum sudanense, at present, kaoliang aphid life table experimental method is tested using excised leaf in culture dish, is adopted
The shortcoming existed with excised leaf is：Water deficient after leaf abscission plant, and nutriment conversion is obstructed, nutrient and moisture with
Fresh and alive plant, which is compared, has certain gap, it is impossible to animation of the accurate simulation kaoliang aphid on sorghum plant.Therefore, in experiment
Room carries out trial test discovery, and kaoliang aphid survival rate in vitro Sorghum Leaves and stem is relatively low.
The content of the invention
It is an object of the invention to provide a kind of kaoliang aphid life table experimental method, this method can be the sorghum in experiment
Aphid provides sufficient moisture and nutrient, and the state of accurate simulation kaoliang aphid free living on field plant can finally obtain standard
True test data.
To achieve the above object, a kind of kaoliang aphid life table experimental method that the present invention is provided has steps of：
A kind of kaoliang aphid life table experimental method, it is characterised in that have steps of：（1）Select open topped and transparent device
Ware, the depth that vessel inner chamber has is 15-20cm, and a window is outputed on the top of vessel, two are outputed in the bottom of vessel
Air-vent above, adds the soil containing Nutrition Soil in vessel, and the height of soil is the 1/4-1/3 of vessel depth of inner cavity；
（2）The sorghum seeds of more than two are embedded in soil, after sorghum emerges, a Sorghum Seedlings are retained by thinning；（3）In soil
Surface covers virgin paper sheet, and causes Sorghum Seedlings to pass through virgin paper sheet；（4）When Sorghum Seedlings length is to 5cm, female aphid of kaoliang aphid is connect, 6 is small
When after by female aphid reject stay young aphid；（5）Single seedling list aphid carries out life table data record, observes within every 12 hours young aphid once, every time
During observation, the young aphid survival condition of record, cast off a skin time and number of times, and the removal of casting off a skin that young aphid on virgin paper sheet will be fallen, work as son
During aphid length to adult, observation in every 24 hours once, when observing every time, records aphid survival condition, production aphid quantity, and it is produced
Young aphid, which removes, to be abandoned, untill aphid is dead.
From the above method, Sorghum Seedlings can provide the moisture and nutrient of abundance for the kaoliang aphid in experiment, be accurate mould
The state of Sorghum propinquum aphid free living on the sorghum plant of field provides condition, it is to avoid kaoliang aphid survival rate is too low in research, and
Cause the problem of experiment can not continue；Aphid bits of casting off a skin can fall on virgin paper sheet, for accurate observation, record；Vessel can
Ensure that casting off a skin for kaoliang aphid is not influenceed by wind inside growth cabinet and float, it is ensured that accurate recording aphid casts off a skin quantity；It is logical
Crossing transparent vessel and window conveniently can intuitively be observed.
The method that the present invention is provided is simple, reliable, implements easy, easy to operate, and can ensure that acquisition accurately experiment number
According to.
Brief description of the drawings
Fig. 1 is the structural representation of vessel used in the present invention.
Embodiment
The present invention is further illustrated with reference to embodiments.
In the present embodiment, selected vessel are made up of commercially available mineral water bottle transformation, and specific way is：By mineral water
Conical portion of the bottle close to bottleneck position is cut off, and retains cylindrical part.
Complete after above-mentioned selection, the present embodiment can be carried out according to the following steps：
（1）, referring to Fig. 1, the depth that the inner chamber of vessel 1 has be 15-20cm, a window 1a is outputed on the top of vessel 1, in device
The bottom of ware 1 is outputed in more than two air-vent 1b, the present embodiment, and the air-vent 1b that the bottom of vessel 1 is outputed is five, in device
High 10cm, a width of 2cm for the window 1a that the top of ware 1 is outputed, add the soil containing Nutrition Soil in vessel, and the height of soil is
The 1/4-1/3 of vessel depth of inner cavity；
（2）, the sorghum seeds of more than two are embedded in soil, it is one high by thinning reservation after sorghum emerges by cultivating
Fine strain of millet seedling；
（3）In Waterlogged soil virgin paper sheet, and Sorghum Seedlings are caused to pass through virgin paper sheet；
（4）When Sorghum Seedlings length is to 5cm, female aphid is connect, female aphid is rejected after 6 hours and stays young aphid；
（5）Single seedling list aphid carries out life table data record, and the young aphid of observation in every 12 hours once, when observing every time, deposit by the young aphid of record
Situation living, cast off a skin time and number of times, and the removal of casting off a skin that young aphid on virgin paper sheet will be fallen, when young aphid length is to adult, every 24
Hour observation once, when observing every time, record aphid survival condition, production aphid quantity, and aphid that it is farrowed removes discarding, up to
Untill aphid is dead.
Claims (2)
1. a kind of kaoliang aphid life table experimental method, it is characterised in that have steps of：（1）Select open topped and transparent
Vessel, the depth that vessel inner chamber has is 15-20cm, and a window is outputed on the top of vessel, two are outputed in the bottom of vessel
Air-vent more than individual, adds the soil containing Nutrition Soil in vessel, and the height of soil is the 1/4-1/ of vessel depth of inner cavity
3；（2）The sorghum seeds of more than two are embedded in soil, after sorghum emerges, a Sorghum Seedlings are retained by thinning；（3）In soil
Earth surface covers virgin paper sheet, and causes Sorghum Seedlings to pass through virgin paper sheet；（4）When Sorghum Seedlings length is to 5cm, female aphid of kaoliang aphid, 6 are connect
Female aphid is rejected after hour and stays young aphid；（5）Single seedling list aphid carries out life table data record, observes within every 12 hours young aphid once, often
During secondary observation, the young aphid survival condition of record, cast off a skin time and number of times, and the removal of casting off a skin that young aphid on virgin paper sheet will be fallen, when
When young aphid length is to adult, observation in every 24 hours once, when observing every time, record aphid survival condition, production aphid quantity, and by its institute
The aphid that farrows, which removes, to be abandoned, untill aphid is dead.
2. kaoliang aphid life table experimental method according to claim 1, it is characterised in that：The air-vent that vessel bottom is outputed
For five, high 10cm, a width of 2cm of the window that vessel top is outputed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710213362.2A CN106993584A (en) | 2017-04-01 | 2017-04-01 | A kind of kaoliang aphid life table experimental method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710213362.2A CN106993584A (en) | 2017-04-01 | 2017-04-01 | A kind of kaoliang aphid life table experimental method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106993584A true CN106993584A (en) | 2017-08-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710213362.2A Pending CN106993584A (en) | 2017-04-01 | 2017-04-01 | A kind of kaoliang aphid life table experimental method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601483A (en) * | 2018-12-11 | 2019-04-12 | 安阳工学院 | A kind of method for breeding making corn leaf aphids life table |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519494A (en) * | 2016-01-28 | 2016-04-27 | 中国水稻研究所 | Insect and seedling culture device for tests and testing method of device |
-
2017
- 2017-04-01 CN CN201710213362.2A patent/CN106993584A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519494A (en) * | 2016-01-28 | 2016-04-27 | 中国水稻研究所 | Insect and seedling culture device for tests and testing method of device |
Non-Patent Citations (2)
| Title |
|---|
| 杜军利: "紫外线(UV-B)辐射对不同色型豌豆蚜生物学特性的影响", 《中国生态农业学报》 * |
| 杜军利等: "不同温度条件下两种色型豌豆蚜的种群参数", 《草业学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601483A (en) * | 2018-12-11 | 2019-04-12 | 安阳工学院 | A kind of method for breeding making corn leaf aphids life table |
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Application publication date: 20170801 |