CN105519494A - Insect and seedling culture device for tests and testing method of device - Google Patents
Insect and seedling culture device for tests and testing method of device Download PDFInfo
- Publication number
- CN105519494A CN105519494A CN201610058033.0A CN201610058033A CN105519494A CN 105519494 A CN105519494 A CN 105519494A CN 201610058033 A CN201610058033 A CN 201610058033A CN 105519494 A CN105519494 A CN 105519494A
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- honeydew
- seedling
- test
- foster
- arranges
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention relates to an insect and seedling culture device and a testing method of the device and belongs to the technical field of insect testing devices and testing methods. The device comprises a base plate, seedling culture cups, barrel covers and a honeydew measuring bag, wherein the upper part of the base plate is open, hanging holes are formed in upper parts of side walls of the base plate, a water drain port is formed in the bottom of the base plate, and a plastic plug is arranged in the water drain port for sealing; two ends of an iron wire penetrate through the hanging holes to divide the base plate into grid space; one seedling culture cup is fixedly placed in each grid, at least one gap is formed in the top of each seedling culture cup, and stalks or leaves of plants planted in the seedling culture cups are placed at the gaps; to-be-tested insects are placed in the honeydew measuring bag, the honeydew measuring bag is densely covered with small holes, and the honeydew measuring bag is fixedly placed on stalks or leaves of plants at the gaps during usage; upper parts of the seedling culture cups are matched with the barrel covers, and window screen covers are arranged at tops of the barrel covers in a matched manner. The device is simple in structure, convenient to produce and high in practicability.
Description
Technical field
The invention belongs to insect experimental rig and test method technical field, be specially a kind of test and support seedling device and test method thereof with foster worm.
Background technology
Plant resources and insect resources are the huge resources banks of the mankind, huge effect is played in recent years in fields such as biological medicine, food, health products, feed and chemical industry, for excavating plant and the insect one side useful to the mankind further, avoid the harm that it causes, need to carry out molecular biology, the research of ecology etc. aspect.When plant and insect carry out the research of molecular biology aspect, material usage is few, but requires meticulously other can not be had to disturb, and other experimental studies also requirement can manual control plant and the insect growth environment of growing or influence factor.All there is various defect in the method that existing multiple foster seedling supports worm, most importantly cannot effectively avoid external environment to for examination plant and the interference of insect and the inside and outside environment of manual control insect and plant growth.In the middle of the ecological study of the nowadays hemipteran such as plant hopper, the mensuration of extensive use honeydew is as the method indirectly obtaining examination worm food ingestion, and in the assay method of honeydew, topmost problem connects worm difficulty, and worm is not easy to take food on ready plant in examination.The comparatively sturdy plant of forefathers' more options is as the food materials of examination worm, and material is not easily cultivated, and adds workload, and easily causes examination worm to escape.
Summary of the invention
For the above-mentioned problems in the prior art, the object of the invention is to design provides a kind of test to use foster worm to support the technical scheme of seedling device and test method thereof, and its structure is simple, and cost is low, easy to use.
Described one test supports seedling device with foster worm, it is characterized in that comprising chassis, supporting seedling cup, bucket cover and honeydew measurement bag, and chassis is opened upper end structure, and chassis side wall top arranges hanging hole, and tray bottom arranges discharge outlet, arranges plastic plug sealing in discharge outlet; Hanging hole is passed by split into grid space, chassis in iron wire two ends; In each grid, fixed placement supports seedling cup, supports seedling cup top and at least arranges a breach, and stem stalk or the blade of supporting planting plants in seedling cup are placed on gap portions; Place insect to be measured in honeydew measurement bag, honeydew measurement bag gathers arranges aperture, during use honeydew measurement bag be fixed on be placed on gap portions plant haulm or blade on; Support seedling cup top and coordinate bucket cover, bucket cover top is equipped with screen window lid.
Described one test supports seedling device with foster worm, it is characterized in that foster seedling cup top arranges 2-6 breach.
Described one test supports seedling device with foster worm, it is characterized in that arranging through hole bottom foster seedling cup.
Described one test supports seedling device with foster worm, it is characterized in that foster seedling cup top inner wall arranges screw thread, and the corresponding screw thread of bucket cover bottom internal, supports seedling cup, bucket cover threaded.
Described one test supports seedling device with foster worm, and it is characterized in that honeydew measurement bag arranges tear line, the opening part of honeydew measurement bag arranges a lengthy motion picture, a short-movie, and a lengthy motion picture arranges adhesive sticker.
Described a kind of test uses foster worm to support the test method of seedling device, it is characterized in that comprising the following steps:
1) use medium planting plants by each foster seedling cup, then hanging hole is passed by split into grid space, chassis in iron wire two ends, in chassis, inject nutrient solution; The foster seedling cup of fixed placement one in each grid, foster Miao Beishang is equipped with bucket cover, and bucket cover top is equipped with screen window lid; Support seedling cup top and at least arrange a breach, stem stalk or the blade of supporting planting plants in seedling cup are placed on gap portions; During use, place insect to be measured in honeydew measurement bag, honeydew measurement bag gathers arranges aperture, on the stem stalk that honeydew measurement bag is fixed on plant or blade; Test is supported seedling device with foster worm and is assembled complete;
2) test assembled is supported seedling device with foster worm to put into incubator and cultivate, required every test parameters is set, tear honeydew measurement bag after off-test, take out the insect to be measured in honeydew measurement bag, carry out honeydew measurement or research insect life table with electronic balance.
Described a kind of test uses foster worm to support the test method of seedling device, it is characterized in that step 2) in: cultivation temperature, light application time and CO are set in incubator
2concentration, research cultivation temperature, light application time and CO
2the impact that concentration is grown plant and insect growth.
Described a kind of test method tested with supporting worm and support seedling device, is characterized in that step 2) in: add heavy metal or trace element in the nutrient solution in chassis, study its impact on plant or insect.
Above-mentioned one test supports seedling device and test method thereof with foster worm, and this device is applicable to raising and the research of the insect such as rice fulgorid, aphid, is applicable to paddy rice, corn, the cultivation of the crops such as wheat and research.This foster worm is supported seedling device and adopts made of plastic, cost-saving, can single use, reduces cross pollution; This foster worm supports the size of the foster seedling cup of seedling device, chassis and bucket cover, highly, often coils quantity etc. and all can adjust according to test needs, can industrialization, and standardization, seriation manufacture, structure is simple, easy to make, practical.
Accompanying drawing explanation
Fig. 1 is chassis structure schematic diagram of the present invention;
Fig. 2 is iron wire structural representation of the present invention;
Fig. 3 is foster seedling cup structure schematic diagram one of the present invention;
Fig. 4 is foster seedling cup structure schematic diagram two of the present invention;
Fig. 5 is bucket cover structure schematic diagram of the present invention;
Fig. 6 is honeydew measurement bag structural representation of the present invention;
In figure: 1-chassis, 101-hanging hole, 102-discharge outlet, 103-plastic plug, 2-iron wire, 3-support seedling cup, 301-breach, 302-through hole, 4-bucket cover, 401-screen window lid, 5-honeydew measurement bag, 501-aperture, 502-tear line, 503-adhesive sticker, 504-lengthy motion picture, 505-short-movie.
Embodiment
Below in conjunction with Figure of description, the invention will be further described.
As shown in the figure, this test supports seedling device with foster worm, comprises chassis 1, supports seedling cup 3, bucket cover 4 and honeydew measurement bag 5, chassis 1 is opened upper end structure, chassis 1 side wall upper part arranges hanging hole 101, arranges discharge outlet 102 bottom chassis 1, arranges plastic plug 103 and seal in discharge outlet 102; Hanging hole 101 is passed by split into grid space, chassis 1 in iron wire 2 two ends; In each grid, fixed placement supports seedling cup 3, supports seedling cup 3 top and at least arranges a breach 301, and stem stalk or the blade of supporting planting plants in seedling cup 3 are placed on breach 301 position; Place insect to be measured in honeydew measurement bag 5, honeydew measurement bag 5 gathers arranges aperture 501, during use honeydew measurement bag 5 be fixed on be placed on breach 301 position plant haulm or blade on; Support seedling cup 3 top and coordinate bucket cover 4, bucket covers 4 tops and is equipped with screen window lid 401.Support seedling cup 3 top and 2-6 breach 301 is set.Support bottom seedling cup 3 and through hole 302 is set.Support seedling cup 3 top inner wall and screw thread is set, the corresponding screw thread of bucket cover 4 bottom internal, foster seedling cup 3, bucket cover 4 threaded, the cross pollution between insect escape and different insects strain is prevented when can be used for foster worm, bucket cover 4 is transparent material, the ventilative printing opacity of screen window lid 401 at top, can not affect plant and insect growth is grown.Each foster seedling cup 3 has independently bucket cover 4, can raise insect by cuvette one cuvette, be convenient to change without worm seedling, and fluke be convenient.Honeydew measurement bag 5 is arranged tear line 502, the opening part of honeydew measurement bag 5 arranges on lengthy motion picture 504, short-movie 505, lengthy motion picture 504 and arranges adhesive sticker 503.In each grid, fixed placement supports seedling cup 3, can ensure the steady of foster seedling cup 3; Discharge outlet 102 bottom chassis 1 has plastic plug 103 to seal time flat, can extract plastic plug 103 and discharge nutrient solution unnecessary in chassis 1 before changing nutrient solution.The foster seedling cup 3 that this foster worm supports seedling device has two kinds, and a kind of bottom is porose, and a kind of bottom atresia, can add matrix in the foster seedling cup that bottom is porose, draw nutrient solution by the hole of bottom from chassis 1, ensures the growth of cultivating plant; Medium culture plant can be added in the foster seedling cup 3 of bottom atresia.The opening part of honeydew measurement bag 5 arranges on lengthy motion picture 504, short-movie 505, lengthy motion picture 504 and arranges adhesive sticker 503.The opening part of preferably supporting seedling cup 3 all has four breach 301, during honeydew measurement, at least one planting stretches out medium on medium, the stem stalk of plant or blade are placed on breach 301 place, insect to be measured (being generally rice fulgorid or aphid etc.) will be put in honeydew measurement bag 5, after during use, a short-movie 505 licks to surround stem stalk, a lengthy motion picture 504 licks and clings above, honeydew measurement bag 5 is fixed on stem stalk, honeydew measurement bag 5 there is tear line 502, experiment terminates the insects to be measured such as peelable taking-up brown planthopper, carries out honeydew measurement with electronic balance.Honeydew measurement bag 5 is covered with intensive aperture 501, can prevents from measuring in bag and assemble steam interference honeydew measurement.When plant and insect carry out the research of molecular biology aspect, material usage is few, but require meticulously other can not be had to disturb, this foster worm supports seedling device can put into incubator, be suitable for nutrient solution or worm is supported in medium nursery, can ensure that environment is consistent, nutrition is consistent, gets rid of other disturbing factors, can obtain satisfactory plant and supply examination insect, facilitate and follow-uply carry out genome, the research of the aspects such as proteomics.This foster worm supports the research that seedling device also can be used for insect life table, and measure the incubation rate of insect, egg laying amount, survival rate, Life of Adult etc., also can add heavy metal or trace element in nutrient solution or medium, studies its impact on plant or insect.This foster worm is supported seedling device and conveniently puts into incubator and carry out different temperatures, light application time, CO
2the factors such as concentration are on the research of the aspects such as the impact that plant and insect growth grow.This foster worm is supported seedling device and is applicable to rice fulgorid, the raising of the insects such as aphid and research, is applicable to paddy rice, corn, the cultivation of the crops such as wheat and research.This foster worm is supported seedling device and can be finished with plastic, cost-saving, can single use, reduce cross pollution, this foster worm support the foster seedling cup 3 of seedling device, chassis 1 and bucket cover 4 size, highly, often coil quantity etc. and all can adjust according to test needs, can industrialization, standardization, seriation manufacture, structure is simple, easy to make, practical.
Adopt this test to support the test method of seedling device with foster worm, comprise the following steps:
1) use medium planting plants by each foster seedling cup 3, then hanging hole 101 is passed by split into grid space, chassis 1 in iron wire 2 two ends, in chassis 1, inject nutrient solution; The foster seedling cup 3 of fixed placement one in each grid, support on seedling cup 3 and be equipped with bucket cover 4, bucket covers 4 tops and is equipped with screen window lid 401; Support seedling cup 3 top and at least arrange a breach 301, stem stalk or the blade of supporting planting plants in seedling cup 3 are placed on breach 301 position; During use, place insect to be measured in honeydew measurement bag 5, honeydew measurement bag 5 gathers arranges aperture 501, on the stem stalk that honeydew measurement bag 5 is fixed on plant or blade; Test is supported seedling device with foster worm and is assembled complete;
2) test assembled is supported seedling device with foster worm to put into incubator and cultivate, required every test parameters is set, tear honeydew measurement bag 5 after off-test, take out the insect to be measured in honeydew measurement bag 5, carry out honeydew measurement or research insect life table with electronic balance.
Above-mentioned steps 2) in: cultivation temperature, light application time and CO2 concentration are set in incubator, the impact that research cultivation temperature, light application time and CO2 concentration are grown plant and insect growth.
Above-mentioned steps 2) in: add heavy metal or trace element in the nutrient solution in chassis 1, study its impact on plant or insect.
Claims (8)
1. a test supports seedling device with foster worm, it is characterized in that comprising chassis (1), supporting seedling cup (3), bucket cover (4) and honeydew measurement bag (5), chassis (1) is opened upper end structure, chassis (1) side wall upper part arranges hanging hole (101), bottom, chassis (1) arranges discharge outlet (102), arranges plastic plug (103) sealing in discharge outlet (102); Hanging hole (101) is passed by chassis (1) split into grid space in iron wire (2) two ends; In each grid, fixed placement supports seedling cup (3), supports seedling cup (3) top and at least arranges a breach (301), and stem stalk or the blade of supporting seedling cup (3) interior planting plants are placed on breach (301) position; Place insect to be measured in honeydew measurement bag (5), upper the gathering of honeydew measurement bag (5) arranges aperture (501), during use honeydew measurement bag (5) be fixed on be placed on breach (301) position plant haulm or blade on; Support seedling cup (3) top and coordinate bucket cover (4), bucket cover (4) top is equipped with screen window lid (401).
2. a kind of test as claimed in claim 1 supports seedling device with foster worm, it is characterized in that foster seedling cup (3) top arranges 2-6 breach (301).
3. a kind of test as claimed in claim 1 supports seedling device with foster worm, it is characterized in that foster seedling cup (3) bottom arranges through hole (302).
4. a kind of test as claimed in claim 1 supports seedling device with foster worm, it is characterized in that foster seedling cup (3) top inner wall arranges screw thread, and the corresponding screw thread of bucket cover (4) bottom internal, supports seedling cup (3), bucket cover (4) threaded.
5. a kind of test as claimed in claim 1 supports seedling device with foster worm, it is characterized in that honeydew measurement bag (5) arranges tear line (502), the opening part of honeydew measurement bag (5) arranges a lengthy motion picture (504), a short-movie (505), a lengthy motion picture (504) is arranged adhesive sticker (503).
6. adopt a kind of test according to claim 1 to use foster worm to support the test method of seedling device, it is characterized in that comprising the following steps:
1) use medium planting plants by each foster seedling cup (3), then hanging hole (101) is passed by chassis (1) split into grid space in iron wire (2) two ends, in chassis (1), inject nutrient solution; The foster seedling cup (3) of fixed placement one in each grid, support on seedling cup (3) and be equipped with bucket cover (4), bucket cover (4) top is equipped with screen window lid (401); Support seedling cup (3) top and at least arrange a breach (301), stem stalk or the blade of supporting seedling cup (3) interior planting plants are placed on breach (301) position; During use, place insect to be measured in honeydew measurement bag (5), upper the gathering of honeydew measurement bag (5) arranges aperture (501), on the stem stalk that honeydew measurement bag (5) is fixed on plant or blade; Test is supported seedling device with foster worm and is assembled complete;
2) test assembled is supported seedling device with foster worm to put into incubator and cultivate, required every test parameters is set, tear honeydew measurement bag (5) after off-test, take out the insect to be measured in honeydew measurement bag (5), carry out honeydew measurement or research insect life table with electronic balance.
7. a kind of test as claimed in claim 6 uses foster worm to support the test method of seedling device, it is characterized in that step 2) in: cultivation temperature, light application time and CO are set in incubator
2concentration, research cultivation temperature, light application time and CO
2the impact that concentration is grown plant and insect growth.
8. a kind of test as claimed in claim 6 supports the test method of seedling device with supporting worm, it is characterized in that step 2) in: add heavy metal or trace element in the nutrient solution in chassis (1), study its impact on plant or insect.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610058033.0A CN105519494B (en) | 2016-01-28 | 2016-01-28 | A kind of experiment supports seedling device and its test method with foster worm |
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| CN201610058033.0A CN105519494B (en) | 2016-01-28 | 2016-01-28 | A kind of experiment supports seedling device and its test method with foster worm |
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| CN105519494A true CN105519494A (en) | 2016-04-27 |
| CN105519494B CN105519494B (en) | 2018-05-29 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614403A (en) * | 2016-12-30 | 2017-05-10 | 福建省农业科学院水稻研究所 | Rice planthopper breeding and observing apparatus and use method of same |
| CN106993584A (en) * | 2017-04-01 | 2017-08-01 | 安徽科技学院 | A kind of kaoliang aphid life table experimental method |
| CN108287009A (en) * | 2018-04-27 | 2018-07-17 | 福建省农业科学院植物保护研究所 | A kind of planthopper honeydew amount determining device and its application method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2393336Y (en) * | 1999-08-26 | 2000-08-30 | 中国人民解放军军事医学科学院微生物流行病研究所 | Blood supplier for bloodsuckers |
| CN201813732U (en) * | 2010-07-21 | 2011-05-04 | 东北农业大学 | Culturing device for gastrointestinal nematodes |
| CN202664022U (en) * | 2012-08-07 | 2013-01-16 | 云南省农业科学院生物技术与种质资源研究所 | Device suitable for insect feeding and biological observation |
| JP2013111008A (en) * | 2011-11-29 | 2013-06-10 | Hanawa No Kurahashi:Kk | Device for rearing insect |
| CN203262059U (en) * | 2013-05-09 | 2013-11-06 | 北京市农林科学院 | Mini-type insect raising cage for experiments on living plants |
| CN204811555U (en) * | 2015-07-20 | 2015-12-02 | 中国水稻研究所 | Device is simply raised to planthopper short -term |
| CN205511724U (en) * | 2016-01-28 | 2016-08-31 | 中国水稻研究所 | It is experimental with foster seedling device of insect feeding |
-
2016
- 2016-01-28 CN CN201610058033.0A patent/CN105519494B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2393336Y (en) * | 1999-08-26 | 2000-08-30 | 中国人民解放军军事医学科学院微生物流行病研究所 | Blood supplier for bloodsuckers |
| CN201813732U (en) * | 2010-07-21 | 2011-05-04 | 东北农业大学 | Culturing device for gastrointestinal nematodes |
| JP2013111008A (en) * | 2011-11-29 | 2013-06-10 | Hanawa No Kurahashi:Kk | Device for rearing insect |
| CN202664022U (en) * | 2012-08-07 | 2013-01-16 | 云南省农业科学院生物技术与种质资源研究所 | Device suitable for insect feeding and biological observation |
| CN203262059U (en) * | 2013-05-09 | 2013-11-06 | 北京市农林科学院 | Mini-type insect raising cage for experiments on living plants |
| CN204811555U (en) * | 2015-07-20 | 2015-12-02 | 中国水稻研究所 | Device is simply raised to planthopper short -term |
| CN205511724U (en) * | 2016-01-28 | 2016-08-31 | 中国水稻研究所 | It is experimental with foster seedling device of insect feeding |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614403A (en) * | 2016-12-30 | 2017-05-10 | 福建省农业科学院水稻研究所 | Rice planthopper breeding and observing apparatus and use method of same |
| CN106993584A (en) * | 2017-04-01 | 2017-08-01 | 安徽科技学院 | A kind of kaoliang aphid life table experimental method |
| CN108287009A (en) * | 2018-04-27 | 2018-07-17 | 福建省农业科学院植物保护研究所 | A kind of planthopper honeydew amount determining device and its application method |
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| Publication number | Publication date |
|---|---|
| CN105519494B (en) | 2018-05-29 |
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