CN209513606U - A kind of cell metabolism real-time detection and dynamic tampering devic - Google Patents
A kind of cell metabolism real-time detection and dynamic tampering devic Download PDFInfo
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- CN209513606U CN209513606U CN201821498225.4U CN201821498225U CN209513606U CN 209513606 U CN209513606 U CN 209513606U CN 201821498225 U CN201821498225 U CN 201821498225U CN 209513606 U CN209513606 U CN 209513606U
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- culture dish
- cell metabolism
- time detection
- gas sensor
- tampering devic
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- 230000019522 cellular metabolic process Effects 0.000 title claims abstract description 33
- 238000011897 real-time detection Methods 0.000 title claims abstract description 29
- 238000001514 detection method Methods 0.000 claims abstract description 28
- 239000000243 solution Substances 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000002347 injection Methods 0.000 claims abstract description 9
- 239000007924 injection Substances 0.000 claims abstract description 9
- 239000012530 fluid Substances 0.000 claims abstract description 4
- 239000004033 plastic Substances 0.000 claims abstract description 4
- 229920003023 plastic Polymers 0.000 claims abstract description 4
- 239000007789 gas Substances 0.000 claims description 37
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 238000007689 inspection Methods 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 10
- 238000012545 processing Methods 0.000 description 9
- 239000010410 layer Substances 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 210000004881 tumor cell Anatomy 0.000 description 6
- 239000012472 biological sample Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 230000004060 metabolic process Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 229940120124 dichloroacetate Drugs 0.000 description 3
- JXTHNDFMNIQAHM-UHFFFAOYSA-N dichloroacetic acid Chemical compound OC(=O)C(Cl)Cl JXTHNDFMNIQAHM-UHFFFAOYSA-N 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- LKDRXBCSQODPBY-VRPWFDPXSA-N D-fructopyranose Chemical compound OCC1(O)OC[C@@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-VRPWFDPXSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
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- 238000010835 comparative analysis Methods 0.000 description 2
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- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- ZCXUVYAZINUVJD-AHXZWLDOSA-N 2-deoxy-2-((18)F)fluoro-alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H]([18F])[C@@H](O)[C@@H]1O ZCXUVYAZINUVJD-AHXZWLDOSA-N 0.000 description 1
- 208000010507 Adenocarcinoma of Lung Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 230000000973 chemotherapeutic effect Effects 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 238000011982 device technology Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 230000002414 glycolytic effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 201000005249 lung adenocarcinoma Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000002901 radioactive waste Substances 0.000 description 1
- 230000018406 regulation of metabolic process Effects 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model provides a kind of cell metabolism real-time detection and dynamic tampering devic characterized by comprising including feed liquor unit and detection unit；The feed liquor unit is micro constant flow pump；The detection unit includes culture dish, physiological parameter sensors chip and gas sensor, the culture dish is in without top hollow cylinder, its intracavity bottom is equipped with physiological parameter sensors chip, gas sensor is provided with above the culture dish, the gas sensor is fixed by the bracket in the top of culture dish, the culture dish connects micro constant flow pump by plastics fluid injection hose, and the periphery of the detection unit is enclosed with seal case.The utility model effective solution can not simultaneously a variety of physical signs of dynamic detection, containing radioactivity, be difficult to the problems such as accurate injection intervention experiment process.
Description
Technical field
The utility model relates to a kind of cell metabolism real-time detection and dynamic tampering devics, belong to medicine detector device technology
Field.
Background technique
For research cell, the related biological behaviour (such as cell growth metabolism moves feature) of the biological samples such as tissue,
The method for being widely deployed in vitro culture biological sample in culture dish at present is studied.
For example, cultivated in vitro with tumor cell line be current oncology studies one of important method.Experiment shows swollen
Oncocyte intake metabolite (the especially rate of glucose) is closely related with the biological behaviour of tumour cell, clinically
It is related to the sensibility of tumours of chemotherapeutic, so the process of dynamic monitoring tumour cell intake glucose is to research tumour to Guan Chong
It wants.Consumption of glucose output CO under normal cell aerobic conditions2, oxygen-free environment metabolism is slowly；No matter but the aerobic nothing of tumour cell
Oxygen condition obtains energy by glycolytic and generates lactic acid.For the feature, we can be thin in vitro by real-time monitoring
CO in born of the same parents' metabolite2Concentration, lactic acid concn (i.e. pH) can determine its metabolic pathway.
Glucose aerobic reaction formula: C6H12O6+6O2→6CO2+6H2O+36ATP
Glycolysis reaction formula: C6H12O6→2C3H6O3+2ATP
And existing detection means is that radionuclide method (uses labeling reaction, utilizes radioactive ray in gamma-rays detection product
Dosage is to determine reaction rate).Culture dish (ware one end is arranged inlet, and one end is arranged liquid outlet, and slightly below inlet,
Facilitate liquid to flow) in cultivate tumour cell, using miniflow pump to its be dynamically added radioisotope labeling glucose or its
Analog (such as 18F- fluorodeoxyglucose) solution is placed in cell incubator and cultivates；Utilize the gamma-rays being placed in above ware
Dose radiation in instrument dynamic detection cell, to calculate the glucose that the tumor cell line absorbs in different time points
Amount.The method can not detect multiple markers, and radioactive waste matter is not easy to handle since label limits simultaneously；It is not influencing
In the case of metabolic process, it is difficult injection and intervenes metabolic response and detect.
Utility model content
It is above-mentioned to solve the purpose of this utility model is to provide a kind of cell metabolism real-time detection and dynamic tampering devic
Problem.
The utility model adopts the technical scheme that
A kind of cell metabolism real-time detection and dynamic tampering devic characterized by comprising including feed liquor unit and detection
Unit；The feed liquor unit is micro constant flow pump；The detection unit includes culture dish, physiological parameter sensors chip and gas
Sensor, the culture dish are in without top hollow cylinder, and intracavity bottom is equipped with physiological parameter sensors chip, the culture
Gas sensor is provided with above ware, the gas sensor is fixed by the bracket in the top of culture dish, the culture dish
Micro constant flow pump is connected by plastics fluid injection hose, the periphery of the detection unit is enclosed with seal case.
Further, a kind of cell metabolism real-time detection and dynamic tampering devic of the utility model, also has such spy
Sign: the sensor chip includes light addressing point level sensor and Clark electrode, and light addressing point level sensor detects pH value of solution
Value, Clark electrode detection dissolved oxygen concentration.
Further, a kind of cell metabolism real-time detection and dynamic tampering devic of the utility model, also has such spy
Sign: being in hollow cuboid outside the gas sensor, and bottom is equipped with air inlet and gas outlet.
Further, a kind of cell metabolism real-time detection and dynamic tampering devic of the utility model, also has such spy
Sign: where the gas sensor interior is laterally sequentially arranged with illuminating source, polaroid and light source receiving layer.
Further, a kind of cell metabolism real-time detection and dynamic tampering devic of the utility model, also has such spy
Sign: where the illuminating source is infrared light sources.
Further, a kind of cell metabolism real-time detection and dynamic tampering devic of the utility model, also has such spy
Sign: where the top layer of the gas sensor interior is gas detection signals process layer.
Further, a kind of cell metabolism real-time detection and dynamic tampering devic of the utility model, also has such spy
Sign, further includes: thermostat, the culture dish are placed in thermostat.
Further, a kind of cell metabolism real-time detection and dynamic tampering devic of the utility model, also has such spy
Sign, further includes: SD card, storage sensor signal.
Further, a kind of cell metabolism real-time detection and dynamic tampering devic of the utility model, also has such spy
Sign, further includes: display screen shows physiological parameter.
Further, a kind of cell metabolism real-time detection and dynamic tampering devic of the utility model, also has such spy
Sign: gas sensor, culture dish bottom physiological parameter sensors chip connect with display screen by data line.
The beneficial effect of utility model
The cell metabolism real-time detection and dynamic tampering devic and detection method of the utility model, effective solution can not
Simultaneously a variety of physical signs of dynamic detection, containing radioactivity, be difficult to the problems such as accurate injection intervention experiment process.It will measure in solution
Culture dish is made in same chip, using it bottom of as in the sensor integration of different physiological parameters, and gas is arranged above chip and passes
Sensor.In vitro culture, detection and the intervention of biological sample are carried out in the chip surface.
Detailed description of the invention
Fig. 1 is the overall structure block diagram of the utility model；
Fig. 2 is the structural schematic diagram that the utility model uses gas sensor；
Fig. 3 is the top view for the culture dish that the utility model uses；
Fig. 4 is the semi-cutaway for the culture dish that the utility model uses；
Fig. 5 is the utility model overhaul flow chart.
Accompanying drawing number: culture dish 1, gas sensor 2, micro constant flow pump 3, control mainboard 4, SD card 5, display screen 6, into
Port 7, gas outlet 8, illuminating source 9, polaroid 10, light source receiving layer 11, signal processing layer 12, sensor chip 13, sealing
Case 14.
Specific embodiment
Illustrate specific embodiment of the present utility model below in conjunction with attached drawing.
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
As shown in Figures 1 to 5, cell metabolism real-time detection and dynamic tampering devic, comprising: feed liquor unit, detection unit,
Data processing and display unit.
As shown in Figure 1, feed liquor unit uses micro constant flow pump 3.
Detection unit includes culture dish 1, physiological parameter sensors chip 13 and gas sensor 2.
As shown in figure 3, culture dish 1 is in without top hollow cylinder, as shown in figure 4,1 intracavity bottom of culture dish is equipped with life
Manage parameter sensors chip 13.
Physiological parameter sensors chip 13 includes light addressing point level sensor and Clark electrode, and wherein light addressing point passes
Sensor is for detecting solution ph, and Clark electrode is for detecting dissolved oxygen concentration.
The top of culture dish 1 is provided with gas sensor 2, and gas sensor 2 is fixed by the bracket in the upper of culture dish 1
Side.
As shown in Fig. 2, being in hollow cuboid outside gas sensor 2, bottom is equipped with air inlet 7 and gas outlet 8, in
Portion is laterally sequentially arranged with illuminating source 9, polaroid 10 and light source receiving layer 11, and top is gas detection signals process layer 12,
Gas sensor 2 utilizes the light of gas with various molecule absorption specific wavelength using infrared ray as measuring medium using infrared absorption method
Wave property is absorbed the size of intensity by light wave to determine the concentration of the gas molecule.
Culture dish 1 connects micro constant flow pump 3 by plastics fluid injection hose,
The periphery of detection unit is enclosed with seal box 14.
When being detected, it needing for seal box 14 to be placed in thermostat, thermostat can use constant incubator,
Or thermostat water bath.
Data processing and display unit include control mainboard 4, SD card 5 and display screen 6, and SD card 5 is inserted in control mainboard 4,
Control mainboard 4 is connect with the sensor chip 13 of display screen 6 and gas sensor 2,1 bottom of culture dish by data line, is passed through
The various physiologic information data storages of collection are shown in display screen 6 in SD card 5, while by physiological signal after signal processing
On.Signal processing herein can be completed using existing filter, and those skilled in the art is from existing various filters
Processing to physiological signal can be realized with suitable model is chosen in the signal handling equipments such as amplifier.Such as using
SevenExcellenceTMS975pH/ conductivity/dissolved oxygen/ion concentration measurement instrument signal processing system accordingly believed
Number processing.
The test interference method of cell metabolism real-time detection and dynamic tampering devic, as shown in Figure 5, comprising the following steps:
S1. it by cell inoculation to be measured in culture dish 1, while configuring culture solution and intervening anti-metabolite agent；
S2. after cell is adherent, the culture solution in culture dish 1 is sucked out, then culture dish 1 is placed in seal box, and will
Seal box is placed in constant temperature bath；
S3. start micro constant flow pump 3 and quantitatively accurately inject configured culture solution；
S4. start detection unit and signal acquisition unit, with 3~10 seconds/time of detection cell metabolism physiology mistakes of fixed cycle
Journey, every physiologic information is by gas sensor 2,1 bottom sensor chip of culture dish in the gas information and solution discharged
13 capture and form digital signal, obtain cell base metabolic information, store and show via data processing and display unit；
S5. after the completion of information record, other reagent interventions metabolism is injected to 1 quantification of culture dish, while pressing above-mentioned step
Rapid S2-S4 records every terms of information；
S6. cell metabolism can be shown that reagent imitates the regulation of metabolism by comparative analysis by the physiologic information of patients before and after intervention
Fruit.
Specifically, by taking human lung adenocarcinoma cell metabolism as an example, process is as shown in Figure 5.It will be to be measured in specific environment
Cell monolayer is inoculated in culture dish 1, while being configured culture solution and being intervened anti-metabolite agent, and reagent is with DCA (dichloroacetate)
Example, places it in the micro constant flow pump 3 of feed liquor unit；After cell is adherent, original culture solution is sucked out and is placed in culture dish 1
In seal box, starts micro constant flow pump 3 and quantitatively accurately inject configured culture solution；Start detection unit and signal acquisition list
Member detects cell metabolism physiology course, items in the gas information and solution discharged with 3~10 seconds/time of fixed cycle
Physiologic information is captured by gas sensor 2, culture dish bottom sensor chip 13 and forms digital signal, obtains cell base generation
It thanks to information, stores and show via control and display module.
After the completion of cell base metabolic information record, to the injection DCA reagent intervention metabolism of culture dish quantification, press simultaneously
Above-mentioned steps record every terms of information；Reagent can be obtained to generation by comparative analysis by the physiologic information of patients before and after intervention cell metabolism
The regulating effect thanked.
The utility model provide a kind of non-marked can real-time detection biological sample a variety of physiologic informations and intervene its generation
The device thanked.The utility model effective solution can not simultaneously dynamic detection a variety of physical signs, containing radioactivity, be difficult to it is accurate
The problems such as injection intervention experiment process.By the sensor integration for measuring different physiological parameters in solution in same chip, it is with it
Culture dish is made in bottom, and gas sensor is arranged above chip.In vitro culture, the inspection of biological sample are carried out in the chip surface
It surveys and intervenes.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (10)
1. a kind of cell metabolism real-time detection and dynamic tampering devic characterized by comprising
Including feed liquor unit and detection unit；
The feed liquor unit is micro constant flow pump；
The detection unit includes culture dish, physiological parameter sensors chip and gas sensor,
The culture dish is in without top hollow cylinder, and intracavity bottom is equipped with physiological parameter sensors chip, the culture dish
Top be provided with gas sensor, the gas sensor is fixed by the bracket in the top of culture dish,
The culture dish connects micro constant flow pump by plastics fluid injection hose, and the periphery of the detection unit is enclosed with seal box
Body.
2. a kind of cell metabolism real-time detection according to claim 1 and dynamic tampering devic, it is characterised in that:
The physiological parameter sensors chip includes light addressing point level sensor and Clark electrode, light addressing point level sensor inspection
Survey solution ph, Clark electrode detection dissolved oxygen concentration.
3. a kind of cell metabolism real-time detection according to claim 1 and dynamic tampering devic, it is characterised in that:
It is in hollow cuboid outside the gas sensor, bottom is equipped with air inlet and gas outlet.
4. cell metabolism real-time detection according to claim 3 and dynamic tampering devic, it is characterised in that:
Wherein, the gas sensor interior is laterally sequentially arranged with illuminating source, polaroid and light source receiving layer.
5. cell metabolism real-time detection according to claim 4 and dynamic tampering devic, it is characterised in that:
Wherein, the illuminating source is infrared light sources.
6. cell metabolism real-time detection according to claim 4 and dynamic tampering devic, it is characterised in that:
Wherein, the top layer of the gas sensor interior is gas detection signals process layer.
7. cell metabolism real-time detection according to claim 1 and dynamic tampering devic, which is characterized in that further include:
Thermostat, the culture dish are placed in thermostat.
8. cell metabolism real-time detection according to claim 1 and dynamic tampering devic, which is characterized in that further include:
SD card, storage sensor signal.
9. cell metabolism real-time detection according to claim 1 and dynamic tampering devic, which is characterized in that further include:
Display screen shows physiological parameter.
10. cell metabolism real-time detection according to claim 1 and dynamic tampering devic, it is characterised in that:
Gas sensor, culture dish bottom physiological parameter sensors chip connect with display screen by data line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821498225.4U CN209513606U (en) | 2018-09-13 | 2018-09-13 | A kind of cell metabolism real-time detection and dynamic tampering devic |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821498225.4U CN209513606U (en) | 2018-09-13 | 2018-09-13 | A kind of cell metabolism real-time detection and dynamic tampering devic |
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| Publication Number | Publication Date |
|---|---|
| CN209513606U true CN209513606U (en) | 2019-10-18 |
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| CN201821498225.4U Expired - Fee Related CN209513606U (en) | 2018-09-13 | 2018-09-13 | A kind of cell metabolism real-time detection and dynamic tampering devic |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108918451A (en) * | 2018-09-13 | 2018-11-30 | 上海健康医学院 | A kind of cell metabolism real-time detection and dynamic tampering devic and test interference method |
-
2018
- 2018-09-13 CN CN201821498225.4U patent/CN209513606U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108918451A (en) * | 2018-09-13 | 2018-11-30 | 上海健康医学院 | A kind of cell metabolism real-time detection and dynamic tampering devic and test interference method |
| CN108918451B (en) * | 2018-09-13 | 2024-02-13 | 上海健康医学院 | Real-time detection and dynamic intervention device and test intervention method for cell metabolism |
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Granted publication date: 20191018 |