CN114112534A - Intelligent sampling and detecting device suitable for microorganism suspension and solid culture medium - Google Patents
Intelligent sampling and detecting device suitable for microorganism suspension and solid culture medium Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
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- Immunology (AREA)
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- Hydrology & Water Resources (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses an intelligent sampling and detecting device suitable for microorganism suspension and solid culture medium, which comprises an intelligent sample detecting system, a three-dimensional sterilizing bin, a multi-dimensional sampling period controller, a synchronous sampling controller, an image collecting system, a strain identifying device, a sample diluting system and an information storage system; the method comprises the steps that a sample is firstly detected whether to be infected with bacteria or not through a sample intelligent detection system, the sample which is not infected with the bacteria firstly identifies strains and then carries out image acquisition, sampling is carried out according to the set sampling period and sampling time, the sample is diluted according to the detection requirement, the sample is detected, and finally all data are stored into an information storage system; the invention can replace the traditional manual sampling, reduce the contamination rate in the detection process, and the strain identification system can lock the target system and eliminate the mixed bacteria, thereby being more beneficial to eliminating the probability of contamination in the fermentation culture process.
Description
Technical Field
The invention relates to the field of microbial fermentation, in particular to an intelligent sampling and detecting device suitable for microbial suspension and solid culture medium.
Background
The microbial fermentation culture technology is an important research direction at present, during the microbial fermentation culture process, the problems of growth and metabolism of thalli and the influence of different fermentation conditions on microbial fermentation are involved, the fermentation mechanism is complex, parameters of fermentation liquor and products of the fermentation liquor need to be measured, and the measurement result has great significance on the research result.
Because the detection technical conditions are limited, the sampling and detection of the microorganism suspension and the solid culture medium are difficult to be carried out simultaneously, the sampling process basically depends on manual work, mechanical automatic sampling is rarely utilized, the sample detection needs to be carried out in a laboratory after the manual sampling is finished, the strict aseptic operation cannot be ensured, and the sample is wasted if the microorganism is infected; the manual sampling and detection process has limitations, and the high efficiency and accuracy of sampling are difficult to guarantee. Therefore, there is a need for an intelligent sampling and detection device for aseptic sampling of microbial suspensions and solid fermentation processes.
Disclosure of Invention
The invention aims to provide an intelligent sampling and detecting device suitable for microorganism suspension and solid culture medium, so as to ensure that no bacteria and impurities are contaminated in the sampling process, ensure that the detected result is not polluted by microorganisms in the environment, and ensure the accuracy of the measured result and the high efficiency of the detection process.
In order to realize the purpose of the invention, the technical scheme adopted by the invention is as follows:
the utility model provides an intelligence sample and detection device suitable for microorganism suspension and solid medium, sample intellectual detection system include three-dimensional sterilization storehouse, three-dimensional sterilization storehouse be located the equipment rear portion, the ultraviolet radiation lamp is installed in three-dimensional sterilization storehouse top left side, ethanol water spraying system is installed to three-dimensional sterilization storehouse right side, ethanol water spraying system 4 alcohol shower nozzles and alcohol accumulator are equipped with, 4 alcohol shower nozzles be located four angles in three-dimensional sterilization storehouse top, the alcohol accumulator be located the equipment outside, be connected with the alcohol shower nozzle through the rubber tube.
The bottom in three-dimensional sterilization storehouse be equipped with the heater, the heater be equipped with waste pipe way, the top of heater is the bottom in three-dimensional sterilization storehouse promptly, is equipped with netted baffle and is convenient for hold the instrument of required sterilization, the top of heater adopts the back taper design, closely laminates with three-dimensional sterilization bulkhead, and the drain is installed at the back taper top, three-dimensional sterilization storehouse waste pipe way need be through inside the heater, just can arrange to waste liquid collection device, waste pipe adopts high temperature resistant stainless steel T venturi tube through the heater part, and the other end is connected with heater waste pipe way, and the control valve of high temperature resistant high pressure is installed at T venturi tube both ends, and other parts adopt high temperature resistant and acid and alkali's rubber pipeline.
The heater be equipped with high temperature steam pipe way, high temperature steam pipe way install the bottom left and right sides in three-dimensional sterilization storehouse, high temperature steam pipe way 8 air jets altogether, 8 air jets equidistance distribute in three-dimensional sterilization storehouse bottom left and right sides, three-dimensional sterilization storehouse front side install the door of taking the sealing washer, the sealing washer of door adopts anti high temperature material, the door outside is equipped with manipulator II and is responsible for sending into three-dimensional sterilization storehouse with the instrument of required sterilization to and be responsible for the instrument of having gone out the fungus and take out.
Integrated sampling probe include multi-functional sampling tube and self-adaptation sample brush head, multi-functional sampling tube be located the multi-functional sampling tube support in three-dimensional sterilization storehouse in left side, the three-dimensional sterilization storehouse left side wall of multi-functional sampling tube support perpendicular to, the thick one end of multi-functional sampling tube can be connected with manipulator I, manipulator I internally mounted has rubber tube and pressure sensor, confirms the sample volume according to the size of pressure, and the maximum sample volume is no longer than multi-functional sampling tube volume, manipulator I be located the slide bar at equipment top, the slide bar on install slider and spring, manipulator I passes through slider and spring and sliding bar connection, manipulator I on install control scheme, control scheme and operating system be connected, through operating panel control.
Self-adaptation sample brush head adopt the silica gel material, self-adaptation sample brush head be located three-dimensional sterilization storehouse right side self-adaptation sample brush head frame, self-adaptation sample brush head frame adopt the wall-hanging to hang in three-dimensional sterilization storehouse right side wall, self-adaptation sample brush head frame include the trapezoidal shelf of S-shaped couple, the S-shaped couple fix on trapezoidal shelf, self-adaptation sample brush head be on a parallel with trapezoidal shelf and place on the S-shaped couple.
The intelligent detection system's operation host computer, liquid crystal display, the operation host computer include control panel, control panel including total control, mechanical hand control switch, integrated sampling probe control switch, three-dimensional sterilization storehouse control switch, multidimension sample cycle controller switch, sample volume controller switch, sampling point controller switch, multizone sample disk controller switch, sample detection device switch, image acquisition system switch, sample dilution system switch, control panel connecting device's circuit adopt bilateral control, the manipulator remove the circuit for planning the circuit, integrated sampling probe control switch be connected with the pressure sensor in the manipulator I.
The multi-angle sample device is positioned on a multi-zone sample disc, and the multi-zone sample disc comprises a shake flask fixing device at the upper part of the left side, a flat plate fixing device at the lower part of the left side, the multi-angle sample device at the upper part of the right side and a test tube fixing frame at the lower part of the right side; the bottom of the multi-angle sample device is provided with an angle controller, the control angle of the angle controller is four gears of 30 degrees, 45 degrees, 60 degrees and 90 degrees, and the required degrees are input on a control panel to control the gears to change the angle of the multi-angle sample device; the bottom of the test tube fixing frame of the multi-region sample disc is provided with a rotator for controlling the direction, and the rotators are connected with a control panel through a circuit and rotate along the clockwise direction; the shake flask fixing device comprises screws and extensible iron wire mesh-shaped fixers, the flat plate fixing device comprises screws and extensible buckle fixers, and the multi-angle sample device and the test tube fixing frame are made of stainless steel materials.
The camera and the illuminating lamp of the image acquisition system are arranged at the bottom of the multi-zone sample tray.
The strain identification system and the sample dilution system are connected with the host; the strain identification system compares the picture shot by the image acquisition system with the characteristic information of the target strain in the information storage system to judge whether strains other than the target strain are generated; the volume of the deionized water volumetric flask and the physiological saline volumetric flask of the sample dilution system are both 500ml, the deionized water volumetric flask and the physiological saline volumetric flask are added by adopting a method of adding solution, a liquid sensor is arranged in the volumetric flask, and when the volume of the solution is less than 100ml, an alarm gives an alarm.
The invention has the beneficial effects that:
the invention is an intelligent sampling and detecting device suitable for microorganism suspension and solid culture medium, which can replace the traditional manual sampling and has small risk of contamination in the detection process; the sterilization system can achieve better sterilization effect aiming at the sterilization method of the mould and the fungus, and the method adopts the mode of externally connecting the solution, so that the solution is easy to replace; the drying performance of the three-dimensional sterilization bin can be ensured by the design of the sewage discharge pipeline of the heater; the integrated sampling probe is divided into a multifunctional sampling tube and a self-adaptive sampling brush head, so that the requirements of shaking bottle sampling and flat plate sampling can be met; the multi-angle sample device is suitable for reading scales at 30 degrees, 45 degrees, 60 degrees and 90 degrees, and is more suitable for reading when storing fermentation liquor; the invention can also detect the reducing sugar content of the fermentation sample and measure the biomass of the thallus; the invention can also collect images in the fermentation process, the camera can automatically focus, and the taken pictures can be automatically added with watermarks and dates and made into slides, thereby being more beneficial to observing the growth condition of thalli; the strain identification system can lock a target system and eliminate mixed bacteria, and is more favorable for eliminating the probability of bacterial contamination in the fermentation culture process; the sample dilution system adopts a gradient dilution method, is diluted by deionized water and normal saline, and can be selected according to experiment requirements.
Drawings
To further illustrate the technical solutions in the embodiments of the present invention, the drawings in the following embodiments will be briefly described. The drawings are only some embodiments of the invention.
FIG. 1 is a schematic flow chart of an intelligent sampling and detection device for microorganism suspension and solid culture medium in the embodiment of the invention.
FIG. 2 is a flow chart of the multi-dimensional sampling period controller according to an embodiment of the invention.
FIG. 3 is a flow chart of a synchronous sampling controller system according to an embodiment of the present invention.
Detailed Description
For further detailed description of the present invention, the detailed description will be made on the embodiments of the present device with reference to the accompanying drawings, and the embodiments described are only a partial example of the operation of the present device.
As shown in figure 1, an intelligent sampling and detecting device suitable for microorganism suspension and solid culture medium comprises an intelligent sample detecting system, a three-dimensional sterilizing bin, a multi-dimensional sampling period controller, a synchronous sampling controller, an image acquisition system, a strain identification device, a sample diluting system, an information storage system, a multi-region sample disc and a multi-angle sample device.
As shown in fig. 2, the multi-dimensional sampling period controller is initialized by the system, sets a sampling flag, calibrates the time of the multi-dimensional sampling period controller after success, updates the data of the multi-dimensional sampling period controller, sets a sampling period after success, selects a working mode, and enters a sleep state after completion of working; the working modes comprise a fermentation liquor sampling mode, a flat plate sampling mode and a test tube sampling mode.
As shown in fig. 3, after the synchronous sampling controller system is initialized, a sampling signal is set, the sampling signal includes a sampling point and a sampling amount, and the system receives and analyzes the sampling signal, transmits a correct sampling signal to the host, and performs a sampling operation.
Preferably, the intelligent detection device comprises: the system comprises an operation host, a liquid crystal display screen, a data input device, a manipulator, a master control switch, an integrated sampling probe control switch, a three-dimensional sterilization bin control switch, a multi-dimensional sampling period controller switch, a sampling volume controller switch, a sampling point controller switch, a multi-region sample disk controller switch, an image acquisition system switch and a sample dilution system switch; through intellectual detection system control sampler, external instrument adopts the rubber tube as defeated appearance pipe, and defeated appearance pipe's introduction port is Y shape interface, and the fermentation liquor can external two kinds of instruments detect simultaneously.
Preferably, the image acquisition system comprises: the camera with the automatic focusing function adopts antibacterial waterproof materials, is an H-shaped camera capable of stretching, can automatically focus, shoots multiple groups of pictures with watermarks according to shooting requirements, selects the clearest picture to be stored in an information storage system according to pixels and resolution ratio, and can make the stored picture into an electronic slide after the whole fermentation process is finished, thereby visually displaying the whole fermentation culture process.
Preferably, the species identification device comprises: the strain screening mechanism judges strains according to the information in the database, analyzes whether a culture medium is infected with the strains or not, and if so, sends the infected strains to the three-dimensional sterilization bin for sterilization treatment.
Preferably, the sample dilution system comprises: the sample dilution system dilutes a sample by a gradient dilution method according to a set dilution multiple, and the diluted sample is sent to a sample detection system for detection.
Preferably, the multi-angle sampler comprises: scale marks are marked at four angles of 30 degrees, 45 degrees, 60 degrees and 90 degrees, the read scales correspond to the horizontal liquid level scale marks of the inclination angle, the upper end of the multi-angle sample device is square, the lower end of the multi-angle sample device is conical, and the scale marks range from 0ml to 10ml and are accurate to 0.01 ml.
Example 1
The concrete implementation mode of taking the Aureobasidium pullulans fermentation liquid out of the fermentation bottle is as follows:
placing an aureobasidium pullulans fermentation culture medium to be sampled on a multi-region sample tray, setting the sampling period to be 0-9 days, the sampling time to be 10:00 per day and the sampling amount to be 500 mu l, adjusting a sampling point according to the volume of fermentation liquor, establishing a related folder for data storage after successful setting, and only selecting the parameters of the folder after the initialization of subsequent sampling equipment; the intelligent detection device checks whether the fermentation liquor is infected with bacteria, if the infected bacteria is conveyed to the three-dimensional sterilization bin for sterilization, the fermentation liquor image collection is carried out without the infected bacteria, the sampling operation is carried out, the obtained sample is placed in a sample device and is conveyed to a sample dilution system for dilution, 300 mu l of the fermentation liquor is diluted by 10 times, the diluted fermentation liquor is conveyed to a sample detection system for biomass and sugar content detection, the fermentation liquor is sterilized after the detection is finished, and the data of the whole detection process is stored in a folder named by strains and fermentation time and is stored in an information storage system.
Example 2
The specific implementation mode of taking the Mortierella raman from a plate culture dish is as follows:
when the direction of the self-adaptive sampling brush head (13) is vertical to the direction of the multi-area sample disc (7), the self-adaptive sampling brush head (13) is used for sampling spores in a solid plate culture dish, the self-adaptive sampling brush head (13) is in contact with spore colonies for 3 seconds, and the self-adaptive sampling brush head (13) adsorbed with the spores is transferred to a dilution system to dilute spore suspension after sampling is completed. Sterilizing a multi-region sample disc by absolute ethyl alcohol, setting a sampling period to be 0-8 days, setting the sampling time to be 10:00 per day, when an adaptive sampling brush head is vertical to the direction of the multi-region sample disc, sampling spores in a solid plate culture dish, checking whether the solid plate culture dish of the Mortierella marburgensis is infected with bacteria by an intelligent detection device, if the infected bacteria is sent to a three-dimensional sterilization bin for sterilization, carrying out the acquisition of images of the Mortierella marburgensis plate without being infected with the bacteria, keeping the adaptive brush head in contact with spore colonies for 3 seconds, transferring the adaptive sampling brush head absorbed with the spores after sampling to a dilution system for dilution of spore suspension, preparing the spore suspension, sending the spore suspension to a sample detection system for biomass and sugar content detection, sterilizing the spore suspension after detection, storing the data of the whole detection process in a folder named by strains and fermentation time, and stored in the information storage system.
Example 3
The specific embodiment of taking the Sporobolomyces from the slant culture medium is as follows:
sterilizing a multi-region sample tray by using absolute ethyl alcohol, setting a sampling period to be 0-5 days, setting a sampling time to be 10:00 per day, placing an inclined plane culture medium on the multi-region sample tray in an inclined way of 45 degrees, setting the height from the lower side of the inclined plane culture medium to the multi-region sample tray to be 5cm, checking whether the inclined plane culture medium of the sporotrichinella throws is infected with bacteria by using an intelligent detection device, if the infected bacteria are sent to a three-dimensional sterilization bin for sterilization, carrying out image acquisition on the inclined plane culture medium of the sporotrichinella throws when the infected bacteria do not exist, setting a sampling point according to 45 degrees, and controlling a sampling needle to incline to 45 degrees by using a mechanical arm to be parallel to the direction of a test tube; the sampling needle is stretched into the test tube to scrape bacterial colony, the sampled sample is set inside the sample container, the adaptive sampling brush head with sporogenous yeast colony is diluted with normal saline to produce sporogenous yeast suspension, which is then fed to the sample detecting system for biomass and sugar content detection.
Claims (10)
1. An intelligent sampling and detection device suitable for microbial suspensions and solid media, characterized in that it comprises an integrated sampling probe (1); the apparatus comprises a multi-angle sampler (2); the device comprises a sample intelligent detection system (3); the device comprises a three-dimensional sterilization bin (4); the apparatus comprises a multi-dimensional sampling period controller (5); the apparatus comprises a synchronous sampling controller (6); the device comprises a multi-zone sample tray (7); the device comprises an image acquisition system (8); the device comprises a strain identification device (9); the apparatus comprises a sample dilution system (10); an information storage system (11); a multifunctional sampling tube (12); an adaptive sampling brush head (13); a multi-mode sterilization function (14).
2. The intelligent sampling and detection device suitable for the microorganism suspension and the solid culture medium is characterized in that the integrated sampling probe (1) of the device is divided into two types, both adopt high-temperature and high-pressure resistant materials, and can be detached and replaced, one type is a multifunctional sampling tube (12) suitable for the fermentation suspension, the multifunctional sampling tube (12) of the device is positioned in a left sampling tube rack in the three-dimensional sterilization bin (4), and the sampling tube rack is vertical to the left side wall of the three-dimensional sterilization bin (4); the multifunctional sampling tube (12) of the device is in a water drop shape, the interior of the multifunctional sampling tube is divided into three small 3ml grids, the inner wall of the multifunctional sampling tube is made of hydrophobic materials, the external part of the multifunctional sampling tube (12) is marked with a No. 1 grid, a No. 2 grid and a No. 3 grid in the anticlockwise direction, the multifunctional sampling tube (12) adopts a three-point sampling method, namely samples are taken at three positions of the surface, the middle and the bottom of a suspension, and then are sequentially stored in the No. 1 grid, the No. 2 grid and the No. 3 grid of the multifunctional sampling tube (12); a pressure sensor with the precision of 0.1% FS is arranged on a multifunctional sampling tube (12) of the device and used for sampling control, the sampling volume is calculated according to the pressure when the multifunctional sampling tube (12) extends into a liquid surface, the precision of the sampling volume is controlled under the same pressure, the thick end of the multifunctional sampling tube (12) of the device is connected with a manipulator, the scale range of the multifunctional sampling tube (12) is 0-10ml, and zero scale marks are positioned at the thin end of the multifunctional sampling tube (12); the other is a self-adaptive sampling brush head (13) suitable for a plate culture dish and a slant culture medium, and the self-adaptive sampling brush head (13) is positioned on a sampling brush head frame at the right side in the three-dimensional sterilization bin (4); the sampling device can be provided with 10 integrated sampling probes (1) at one time, the 10 integrated sampling probes (1) are connected by a series connection method or a parallel connection method, the series connection method is only suitable for taking 0-10 probes of the same kind, the parallel connection method is simultaneously suitable for two probes, namely 0-5 multifunctional sampling tubes (12) and 0-5 self-adaptive sampling brush heads (13), when the parallel connection method is adopted, the two probes must be both odd numbers or even numbers, and the maximum number of each probe is less than or equal to 5.
3. The intelligent sampling and detection device for microorganism suspension and solid culture medium as claimed in claim 1, characterized in that the multi-angle sampler (2) of the device has calibration lines at four angles of 30 °, 45 °, 60 °, 90 °; the multi-angle sampler (2) upper end of device is for the square form lower extreme to be conical, and the square four sides of multi-angle sampler (2) have four scales, and zero scale mark is the same and the scale scope is 0-10ml accurate to 0.01ml, and sample test tube front side is the scale of perpendicular to horizontal plane, and sample test tube right side is the scale of slope 60, and sample test tube rear side is the scale of slope 45, and sample test tube left side is the scale of slope 30, and the scale of reading corresponds inclination's horizontal liquid level scale mark.
4. An intelligent sampling and testing device for microbial suspensions and solid media as claimed in claim 1, said intelligent testing system (3) being characterized by: the intelligent detection system comprises an operation host, a liquid crystal display screen, a data entry device, a manipulator, a master control switch, an integrated sampling probe (1) control switch, a three-dimensional sterilization bin (4) control switch, a multi-dimensional sampling period controller (5) switch, a sampling quantity controller switch, a sampling point controller switch, a multi-zone sample disk controller (8) switch, an image acquisition system (8) switch and a sample dilution system (10) switch, wherein when the intelligent detection system (3) detects the content of glucose and ethanol, an external biosensor is adopted, a sample conveying pipe is adopted to convey liquid to be detected to the external biosensor, and a Y-shaped interface is adopted at the sample conveying pipe at a sample inlet; the sample conveying pipe of the external instrument can be externally connected with other instruments according to the detection requirement.
5. The intelligent sampling and detection device suitable for microorganism suspension and solid fermentation according to claim 1, characterized in that the multi-mode sterilization function (14) of the three-dimensional sterilization chamber (4) of the device comprises an ultraviolet sterilization mode and a high-pressure steam sterilization mode, the top of the three-dimensional sterilization chamber (4) of the device is provided with an ultraviolet irradiation lamp with the length of 20cm at a position 2cm away from the left side wall, and an ethanol spraying system is arranged at a position 2cm away from the right side wall; the device adopts 30W ultraviolet radiation lamp in ultraviolet sterilization mode, the wavelength of ultraviolet is 253.6nm, and the ultraviolet radiation dose is 325.9J/m2，The temperature of the three-dimensional sterilization bin (4) is controlled to be 31.5 ℃, and the humidity is 50 percent; the spraying system directly uses 75.5% ethanol for sterilization for 5min aiming at bacteria, adopts a gradient sterilization method aiming at mould, and needs to use 70.5% ethanol for sterilization for 5min, then use 73.0% ethanol for sterilization for 5min, and then use 75.5% ethanol for sterilization for 5min, wherein the humidity of the three-dimensional sterilization chamber (4) is 50%; the high-pressure steam sterilization mode of the device is suitable for sterilization of agar culture media, fermentation liquor and the integrated sampling probe (1), high-temperature steam pipelines are installed on the left side and the right side of the bottom of the three-dimensional sterilization bin (4), a bin gate with a sealing ring is arranged on the front side, a closed space is formed during sterilization, and the initial temperature is 121.5 ℃ and the pressure is 101.35 kPa.
6. The intelligent sampling and detection device for microorganism suspension and solid culture medium according to claim 1, characterized in that the synchronous sampling controller (6) of the device comprises a sampling point and a sampling amount control, the front side of the multi-angle sampler (2) of the device is provided with a scale vertical to the horizontal plane, the right side of the multi-angle sampler (2) is provided with a scale inclined by 60 degrees, the rear side of the sample test tube is provided with a scale inclined by 45 degrees, and the left side of the sample test tube is provided with a scale inclined by 30 degrees; scales are selected according to the volume of fermentation liquor in the multi-angle sample device (2), the volume of the fermentation liquor is from high to low, the corresponding scales are selected from 30 degrees to 90 degrees, and the precision range of the sampling volume of the fermentation liquor is 0.0005-20 ml; the height from the lower side of the test tube which is inclined upwards by 45 degrees to the multi-zone sample tray (7) is 5cm, a sampling point is set according to 45 degrees, and a mechanical arm controls the sampling needle (13) to incline by 45 degrees and be parallel to the direction of the test tube; the height of a self-adaptive sampling brush head (13) from a multi-region sample disc (7) is 4cm, the bottom of a fermentation bottle is attached to the multi-region sample disc (7), a mechanical arm controls a multifunctional sampling tube (12) to vertically extend into the fermentation bottle along with the multi-region sample disc (7), a vertical distance three-point sampling method is adopted for sampling, the sampling tube starts to sense the height of fermentation liquor for the first time and samples when dropping to 2.5cm from the multi-region sample disc, the sampling tube senses the height of the fermentation liquor for the second time and samples when dropping to 1.8cm from the multi-region sample disc, the sampling tube senses the height of the fermentation liquor for the third time and samples when dropping to 0.9cm from the multi-region sample disc, and the sampling rate is 2.5 ml/s; when the height of the fermentation liquid level is less than 1.8cm of the multi-zone sample tray, the multifunctional sampling tube (12) repeatedly blows and sucks 3 times to uniformly mix the fermentation liquid and then samples once; when the direction of the self-adaptive sampling brush head (13) is vertical to the direction of the multi-area sample disc (7), the self-adaptive sampling brush head (13) is used for sampling spores in a solid plate culture dish, the self-adaptive sampling brush head (13) is in contact with spore colonies for 3 seconds, and the self-adaptive sampling brush head (13) adsorbed with the spores is transferred to a dilution system to dilute spore suspension after sampling is completed.
7. An intelligent sampling and testing device for microorganism suspension and solid media according to claim 1, characterized in that the multi-zone sample tray (7) of the device is divided into four 0.16m2The upper part of the left side is a shaking bottle fixing device, the lower part of the left side is a solid plate culture dish fixing device, the upper part of the right side is a multi-angle sample device (2), and the lower part of the right side is a test tube fixing frame; the scale range of the multi-angle sampler (2) is 0-10ml, the precision of 0-1ml is 0.01ml, the precision of 0-10ml is 0.1ml, a zero scale mark is positioned at the bottom of the front end of the sampler (2), and the first sampler on the left side is a reference sampler (13); the circulator of control direction is installed to the test tube mount bottom of multizone sample dish (7), conveniently reads different inclination's scale, and test tube mount hack lever adopts 2 flexible pipe control test tube height and gradient, and inclination is 30 °, 45 °, 60 °, 90 °, 180 °, and 180 is the state of not depositing the sample.
8. The intelligent sampling and detection device for microorganism suspension and solid culture medium as claimed in claim 1, wherein the image acquisition system (8) of the device is characterized in that the camera can automatically focus, the type of bacteria is selected on the host, the watermark of the type of bacteria and the shooting date is generated on the shot picture, then the picture is classified and stored according to the type of bacteria, the types of bacteria are stored according to the time sequence by adopting the principle of proximity, and a folder named by the bacteria is established; the camera and the illuminating lamp are arranged at the bottom of the multi-zone sample disc (7), according to the set shooting period and the shooting position, the shooting position can be arranged at the bottom of the fermentation bottle, the front and the back of the fermentation bottle and the surface of the culture medium, the camera adopts an antibacterial waterproof material and is a telescopic H-shaped camera, the telescopic length is 20cm-100cm, the rotation angle is 0-360 degrees, the camera can automatically focus, the fermentation broth of the culture medium can extend into the culture medium when being shot, when a plate culture dish and a test tube culture medium are shot, bacterial colonies can be shot in an amplifying way, 5 groups of pictures can be continuously shot at the same position within 2 seconds, after being screened by an image acquisition system (8), the clearest picture is automatically selected and stored according to pixels and resolution ratio, after the fermentation process is finished, the stored pictures are made into electronic slides according to the shooting time sequence, the process that the bacteria at the position grow from small size to decline can be observed during playing, and then sent to the information storage system (11).
9. An intelligent sampling and testing device for microorganism suspension and solid culture medium as claimed in claim 1, wherein the strain identification system (9) of said device can screen the sample culture medium, automatically eliminate the mixed bacteria except the target strain, and the contaminated culture medium is sent to the three-dimensional sterilization chamber (4) for sterilization; the device comprises an information storage device (11) and a data entry device, wherein a hard disk is stored; the color, density and diameter of a microbial suspension or the diameter, the degree of swelling, the edge shape, the surface smoothness, the surface color, the transparency and other strain information of a colony on solid culture are automatically introduced into an information storage device (11) through an image recognition system, manual editing and modification can be carried out after the automatic introduction, an information base named by the strain name is established, the type of the strain is recognized according to the information base, and the type of an integrated sampling probe (1) is selected according to the physical form of a sample; before sampling, sampling interval time is set through a multi-dimensional sampling period controller (5), the intelligent sample detection system (3) detects the concentration of glucose, the concentration of xylose, the concentration of lactose, the concentration of ethanol and the concentration of glutamic acid after sampling the microorganism suspension, and respectively draws a relation graph of the concentration of glucose, the concentration of xylose, the concentration of lactose, the concentration of ethanol and the concentration of glutamic acid in the suspension with time according to a plurality of sampling results and records the relation graph in an information base.
10. The intelligent sampling and testing device for microorganism suspension and solid culture medium as claimed in claim 1, wherein: a sample dilution system (10) of the device comprising a volumetric flask of deionized water and a volumetric flask of physiological saline, the saline concentration of the physiological saline being 0.84%; a sample dilution system (10) of the device manually sets a dilution multiple on a host, the set dilution multiple is led into a data entry device, a sample is diluted by deionized water according to the set dilution multiple, the sample is uniformly dispersed in a water phase by vibration, a gradient dilution method is adopted, 9.9ml of deionized water is added into 0.1ml of the sample to prepare a 1:10 diluted sample, the diluted sample of 1:10 and 9.9ml of deionized water are added after the uniform vibration to prepare a 1:100 diluted sample, the target diluted sample is subjected to gradient dilution according to requirements, and the target diluted sample is sent to a sample detection system (3) for detection.
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