CN206396253U - A kind of cell co-culture device - Google Patents

A kind of cell co-culture device Download PDF

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Publication number
CN206396253U
CN206396253U CN201720030185.XU CN201720030185U CN206396253U CN 206396253 U CN206396253 U CN 206396253U CN 201720030185 U CN201720030185 U CN 201720030185U CN 206396253 U CN206396253 U CN 206396253U
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CN
China
Prior art keywords
articulamentum
culture
bag main
cell
culture bag
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201720030185.XU
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Chinese (zh)
Inventor
牟丽莎
蔡志明
张军方
赵成江
王雨
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen Second Peoples Hospital
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Shenzhen Second Peoples Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Shenzhen Second Peoples Hospital filed Critical Shenzhen Second Peoples Hospital
Priority to CN201720030185.XU priority Critical patent/CN206396253U/en
Application granted granted Critical
Publication of CN206396253U publication Critical patent/CN206396253U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model is related to a kind of culture apparatus, is in particular a kind of cell co-culture device, not only can cultivate two kinds of cells simultaneously, and two culture bag main bodys can be used separately, and cell is cultivated using two kinds of different nutrient solutions.Cylinder is in culture bag main body upper end, hole in cylinder is connected with the space in culture bag main body, plug is interference fitted with cylinder, culture bag main body is provided with two, two culture bag main bodys are linked together by articulamentum I with articulamentum II, interface channel is between articulamentum I and articulamentum II, the space of two culture bag body interiors is interconnected with interface channel, one culture bag main body and articulamentum I, there is screen pack I junction of articulamentum II by hot-melt adhesive paste, another culture bag main body and articulamentum I, there is screen pack II junction of articulamentum II by hot-melt adhesive paste, the circular groove on circular projection and articulamentum II on articulamentum I is interference fit, culture bag body interior has pillar by hot-melt adhesive paste.

Description

A kind of cell co-culture device
Technical field
The utility model is related to a kind of culture apparatus, is in particular a kind of cell co-culture device.
Background technology
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology.No matter for Whole biotechnology, or for the biological cloning technology of one of them, cell culture is all an essential mistake Journey, the extensive clone of cell culture inherently cell.Cell culture technology can by a cell by mass propgation into For many cells that are simple unicellular or seldom breaking up, this is the essential link of clone technology, and cell culture is in itself It is exactly the clone of cell.Substantial amounts of cell or its metabolite are obtained by cell culture.Because biological product is all from cell Get, it is possible to which it is most crucial, most basic technology in biotechnology to say cell culture technology.But it is many sometimes for cultivating Cell, the device of the existing few various kinds of cell co-incubations of culture apparatus are planted, so there is this to design.
The content of the invention
The utility model, not only can be thin by two kinds mainly solving the technical problems that provide a kind of cell co-culture device Born of the same parents are cultivated simultaneously, and two culture bag main bodys can be used separately, and cell is cultivated using two kinds of different nutrient solutions.
In order to solve the above technical problems, the utility model is related to a kind of culture apparatus, in particular it is that a kind of cell is total to Culture apparatus, including culture bag main body, cylinder, plug, articulamentum I, pillar, screen pack I, articulamentum II, screen pack II and company Road is connected, not only two kinds of cells can simultaneously be cultivated, and two culture bag main bodys can be used separately, and use two kinds of differences Nutrient solution is cultivated cell.
Circular projection is provided with articulamentum I, circular projection and articulamentum I are an entirety.
Circular groove is provided with articulamentum II.
Culture bag main body is sealing bag, and cylinder is thermally melted in culture bag main body upper end, and the hole in cylinder and culture bag Space connection in main body, plug is interference fitted with cylinder, and culture bag main body is provided with two, and two culture bag main bodys pass through connection Layer I is linked together with articulamentum II, and interface channel is arranged between articulamentum I and articulamentum II, in two culture bag main bodys The space in portion is interconnected with interface channel, and a culture bag main body and the junction of articulamentum I, articulamentum II pass through the company of hot melt Screen pack I is connected to, another culture bag main body has screen pack II with articulamentum I, the junction of articulamentum II by hot-melt adhesive paste, Circular projection on articulamentum I is with the circular groove on articulamentum II to be interference fitted, and culture bag body interior passes through the company of hot melt It is connected to pillar.
As the further optimization of the technical program, the screen pack I described in a kind of cell co-culture device of the utility model Culture cell dia is respectively less than with the filter opening diameter on screen pack II.
As the further optimization of the technical program, the pillar described in a kind of cell co-culture device of the utility model is provided with Two.
As the further optimization of the technical program, the stay material described in a kind of cell co-culture device of the utility model It is duroplasts.
A kind of the utility model cell co-culture device has the beneficial effect that:
A kind of cell co-culture device of the utility model, not only can cultivate two kinds of cells simultaneously, and two cultures Bag main body can be used separately, and cell is cultivated using two kinds of different nutrient solutions.
Brief description of the drawings
The utility model is described in more detail with specific implementation method below in conjunction with the accompanying drawings.
Fig. 1 is a kind of cross section structure diagram of cell co-culture device of the utility model.
Fig. 2 is a kind of structural representation of cell co-culture device of the utility model.
Fig. 3 is a kind of structural representation of articulamentum I of cell co-culture device of the utility model.
Fig. 4 is a kind of structural representation of articulamentum II of cell co-culture device of the utility model.
Fig. 5 is the circular projection and circular groove fit structure schematic diagram of a kind of cell co-culture device of the utility model.
In figure:Culture bag main body 1;Cylinder 2;Plug 3;Articulamentum I 4;Circular projection 4-1;Pillar 5;Screen pack I 6;Connection Layer II 7;Circular groove 7-1;Screen pack II 8;Interface channel 9.
Embodiment
Embodiment one:
Illustrate present embodiment with reference to Fig. 1,2,3,4,5, the utility model is related to a kind of culture apparatus, more specifically Say it is a kind of cell co-culture device, including culture bag main body 1, cylinder 2, plug 3, articulamentum I 4, pillar 5, screen pack I 6, company Layer II 7, screen pack II 8 and interface channel 9 are connect, not only two kinds of cells can simultaneously be cultivated, and two culture bag main bodys can To be used separately, cell is cultivated using two kinds of different nutrient solutions.
Circular projection 4-1 is provided with articulamentum I 4, circular projection 4-1 and articulamentum I 4 are an entirety.
Circular groove 7-1 is provided with articulamentum II 7.
Culture bag main body 1 is sealing bag, and cylinder 2 is thermally melted in the upper end of culture bag main body 1, and the Kong Yupei in cylinder 2 The space connection in bag main body 1 is supported, plug 3 is interference fitted with cylinder 2, and culture bag main body 1 is provided with two, two culture bag main bodys 1 is linked together by articulamentum I 4 with articulamentum II 7, and interface channel 9 is arranged between articulamentum I 4 and articulamentum II 7, and two Space inside individual culture bag main body 1 is interconnected with interface channel 9, a culture bag main body 1 and articulamentum I 4, articulamentum II There is screen pack I 67 junction by hot-melt adhesive paste, another culture bag main body 1 and articulamentum I 4, the junction of articulamentum II 7 There is the circular projection 4-1 on screen pack II 8, articulamentum I 4 to be with the circular groove 7-1 on articulamentum II 7 by hot-melt adhesive paste It is full of cooperation, has pillar 5 by hot-melt adhesive paste inside culture bag main body 1.In use, plug 3 is extracted, by cell to be cultivated from circle Poured into cylinder 2 in culture bag main body 1.During using nutrient solution culture cell of the same race, due to culture bag main body 1 and interface channel 9 it Between be interconnected, make the nutrient solution inside two culture bag main bodys 1 in sustained height, cell to be cultivated poured into two trainings respectively Support in bag main body 1, the effect of screen pack I 6 and screen pack II 8 is, when cultivating different types of cell, to prevent two kinds of cells from mixing Together, poured out after the completion of culture from cylinder 2, the effect of pillar 5 is that two sides for preventing culture bag main body 1 are attached to one Rise, cause part cell not pour out.During using not nutrient solution culture cell of the same race, by circular projection 4-1 and circular groove 7-1 Between coordinate, two culture bag main bodys 1 are separated, it is to avoid two kinds of nutrient solutions mix.
Embodiment two:
Illustrate present embodiment with reference to Fig. 1,2,3,4,5, present embodiment is described further to embodiment one, Described screen pack I 6 is respectively less than culture cell dia with the filter opening diameter on screen pack II 8.
Embodiment three:
Illustrate present embodiment with reference to Fig. 1,2,3,4,5, present embodiment is described further to embodiment one, Described pillar 5 is provided with two.
Embodiment four:
Illustrate present embodiment with reference to Fig. 1,2,3,4,5, present embodiment is described further to embodiment one, The described material of pillar 5 is duroplasts, and the effect support culture bag main body 1 of pillar 5 prevents two sides of culture bag main body 1 from pasting Together, part cell is caused not pour out.
Operation principle of the present utility model is:In use, plug 3 is extracted, cell to be cultivated is poured into training out of cylinder 2 Support in bag main body 1.During using nutrient solution culture cell of the same race, due to being interconnected between culture bag main body 1 and interface channel 9, Make the nutrient solution inside two culture bag main bodys 1 in sustained height, cell to be cultivated is poured into two culture bag main bodys 1 respectively Interior, the effect of screen pack I 6 and screen pack II 8 is, when cultivating different types of cell, to prevent two kinds of cells from mixing, train Poured out after the completion of supporting from cylinder 2, the effect of pillar 5 is that two sides for preventing culture bag main body 1 are sticked together, and causes portion Cell is divided not pour out.During using not nutrient solution culture cell of the same race, it will coordinate between circular projection 4-1 and circular groove 7-1, Two culture bag main bodys 1 are separated, it is to avoid two kinds of nutrient solutions mix.
Certainly, described above is not limitation of the utility model, and the utility model is also not limited to the example above, this skill The variations, modifications, additions or substitutions that the those of ordinary skill in art field is made in essential scope of the present utility model, also belong to In protection domain of the present utility model.

Claims (4)

1. a kind of cell co-culture device, including culture bag main body (1), cylinder (2), plug (3), articulamentum I (4), pillar (5), screen pack I (6), articulamentum II (7), screen pack II (8) and interface channel (9), it is characterised in that:Set on articulamentum I (4) Circular projection (4-1) is equipped with, circular projection (4-1) and articulamentum I (4) are an entirety;
Circular groove (7-1) is provided with articulamentum II (7);
Culture bag main body (1) is sealing bag, and cylinder (2) is thermally melted in culture bag main body (1) upper end, and the hole in cylinder (2) Connected with the space in culture bag main body (1), plug (3) is interference fitted with cylinder (2), culture bag main body (1) is provided with two, two Individual culture bag main body (1) is linked together by articulamentum I (4) with articulamentum II (7), and interface channel (9) is arranged on articulamentum I (4) between articulamentum II (7), the internal space of two culture bag main bodys (1) is interconnected with interface channel (9), a training Support bag main body (1) and articulamentum I (4), the junction of articulamentum II (7) has screen pack I (6) by hot-melt adhesive paste, another training Support bag main body (1) and articulamentum I (4), the junction of articulamentum II (7) has screen pack II (8), articulamentum I by hot-melt adhesive paste (4) the circular projection (4-1) on is with the circular groove (7-1) on articulamentum II (7) to be interference fitted, and culture bag main body (1) is interior There is pillar (5) in portion by hot-melt adhesive paste.
2. a kind of cell co-culture device according to claim 1, it is characterised in that:Described screen pack I (6) and filtering Filter opening diameter on net II (8) is respectively less than culture cell dia.
3. a kind of cell co-culture device according to claim 1, it is characterised in that:Described pillar (5) is provided with two.
4. a kind of cell co-culture device according to claim 1, it is characterised in that:Described pillar (5) material is hard Plastics.
CN201720030185.XU 2017-01-11 2017-01-11 A kind of cell co-culture device Expired - Fee Related CN206396253U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720030185.XU CN206396253U (en) 2017-01-11 2017-01-11 A kind of cell co-culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720030185.XU CN206396253U (en) 2017-01-11 2017-01-11 A kind of cell co-culture device

Publications (1)

Publication Number Publication Date
CN206396253U true CN206396253U (en) 2017-08-11

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Country Status (1)

Country Link
CN (1) CN206396253U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108148759A (en) * 2018-03-02 2018-06-12 河南科技大学 A kind of multipurpose gas-permeable container for attached cell culture/co-cultivation

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108148759A (en) * 2018-03-02 2018-06-12 河南科技大学 A kind of multipurpose gas-permeable container for attached cell culture/co-cultivation
CN108148759B (en) * 2018-03-02 2020-10-30 河南科技大学 Multipurpose ventilating container for adherent cell culture/co-culture

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170811

Termination date: 20180111

CF01 Termination of patent right due to non-payment of annual fee