CN208776740U - High dissolved oxygen glass shaking flask - Google Patents
High dissolved oxygen glass shaking flask Download PDFInfo
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- CN208776740U CN208776740U CN201820515003.2U CN201820515003U CN208776740U CN 208776740 U CN208776740 U CN 208776740U CN 201820515003 U CN201820515003 U CN 201820515003U CN 208776740 U CN208776740 U CN 208776740U
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- shaking flask
- strip
- dissolved oxygen
- bottle
- bottleneck
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Abstract
The utility model discloses a kind of high dissolved oxygen glass shaking flask, including bottleneck, body and bottom of bottle, the bottleneck is circle, bottleneck lower end extends downwardly and the body to form circular cone figure is gradually expanded, the body lower end is formed with circular bottom of bottle, which forms a plurality of strip-shaped baffles of the internal protrusion of oriented shaking flask.The bottleneck is the design of wide-mouth diameter, and the diameter of bottleneck is not less than the 1/3 of body maximum gauge.High dissolved oxygen glass shaking flask provided by the utility model, bottom of bottle are formed with a plurality of strip-shaped baffles of protrusion, are conducive to increase cell dissolved oxygen amount, to achieve the effect that improve cell culture amount；A further improvement is that the bottleneck of simple glass shaking flask is changed to the design of wide-mouth diameter, it can increase the ventilation of shaking flask, be conducive to further increase dissolved oxygen amount.
Description
Technical field
The utility model relates to the experimental instrument and equipments of field of biological pharmacy, and in particular to a kind of height for cell culture
Dissolved oxygen glass shaking flask.
Background technique
Currently, commonly there are three types of the shaking flasks of specification in laboratory: the first is the 2.5L volume for doing High Cell Density And High Expression
High density is overfulfiled a production target shaking flask, is for second the shaking flask that 5L volume is used to cultivate mammalian cell, the third is the glass of 3L volume
Shaking flask.The shaking flask culture cell maximum capacity of these three specifications is 1L；And the first and be all plastics shaking flask second, all belong to
In consumables, using that will scrap after ten times, second is disposable for the first；And first two bottle unit price is expensive, one
A bottle is at 400 yuan or so；Currently, the shaking flask design with capacity does not have one kind that can reach 2L cell culture amount.
Utility model content
Technical problem to be solved by the utility model is to provide a kind of high dissolved oxygen glass shaking flasks, can promote dissolved oxygen
Amount improves cell culture amount.
In order to solve the above technical problems, the utility model provides a kind of high dissolved oxygen glass shaking flask comprising bottleneck, body and
Bottom of bottle, the bottleneck are circle, and the bottleneck lower end extends downwardly and be gradually expanded the body to form circular cone figure, the body
Lower end is formed with circular bottom of bottle, and the bottom of bottle forms a plurality of strip-shaped baffles of the internal protrusion of oriented shaking flask.
Specifically, a plurality of strip-shaped baffles are respectively positioned in the different diametrical directions at round bottle bottom, and two neighboring
Angle between strip-shaped baffle is equal.
Preferably, the strip-shaped baffle is three, and circle is directed toward by the circumference at round bottle bottom in the direction of three strip-shaped baffles
The heart, the angle between three strip-shaped baffles is 120 °.
Preferably, the strip-shaped baffle is four, and circle is directed toward by the circumference at round bottle bottom in the direction of four strip-shaped baffles
The heart, the angle between four strip-shaped baffles is 90 °.
Specifically, the height of shown strip-shaped baffle is h, 0.1mm≤H3≤10mm.Preferably, 0.2mm≤H3≤1mm.
Specifically, the bottleneck is the design of wide-mouth diameter, the diameter of bottleneck is not less than the 1/3 of body maximum gauge.Preferably,
The diameter of the bottleneck is not less than the 2/5 of body maximum gauge.
Specifically, the volume specification of the shaking flask is 5L.
Preferably, the bottleneck diameter is 100mm, and bottom of bottle diameter is 250mm, and strip-shaped baffle height is 0.5mm.
High dissolved oxygen glass shaking flask provided by the utility model, bottom of bottle are formed with a plurality of strip-shaped baffles of protrusion, favorably
In increasing cell dissolved oxygen amount, to achieve the effect that improve cell culture amount；A further improvement is that by simple glass shaking flask
Bottleneck is changed to the design of wide-mouth diameter, can increase the ventilation of shaking flask, is conducive to further increase dissolved oxygen amount.
Detailed description of the invention
Fig. 1 is the schematic perspective view of an embodiment of the present invention.
Fig. 2 is the lateral plan of an embodiment of the present invention.
Fig. 3 is the bottom of bottle bottom view of an embodiment of the present invention.
Sign flag illustrates in attached drawing:
1 is bottleneck；2 be body；3 be bottom of bottle；4 be strip-shaped baffle；H1 is bottleneck height；H2 is shaking flask height；H3 is item
Shape height of baffle plate；R1 is bottom of bottle diameter；R2 is body maximum gauge；R3 is bottleneck diameter；L is the length of strip-shaped baffle；θ is phase
The angle of adjacent strip-shaped baffle.
Specific embodiment
Clear, complete description is carried out to the technical solution of the utility model below in conjunction with attached drawing, it is clear that described
Embodiment is a part of the embodiment of the utility model, instead of all the embodiments.Based on the embodiments of the present invention,
Those of ordinary skill in the art's every other embodiment obtained without making creative work belongs to this
The range of utility model protection.
As shown in Figure 1, the stereochemical structure signal of the specific embodiment for the high dissolved oxygen glass shaking flask of the utility model
Figure.The high dissolved oxygen glass shaking flask includes 3 three parts of bottleneck 1, body 2 and bottom of bottle；Wherein, bottleneck 1 is circle, the lower end of bottleneck 1
Extend downwardly and be gradually expanded the body 2 to form circular cone figure, the lower end of body 2 is formed with circular bottom of bottle 3, bottom of bottle 3 it is upper
Surface forms a plurality of strip-shaped baffles 31 of the internal protrusion of oriented shaking flask.In a particular embodiment, a plurality of bar shaped gears
Plate 31 is respectively positioned in the different diametrical directions at round bottle bottom 3, and the angle theta between two neighboring strip-shaped baffle 31 is equal.
As shown in Fig. 2, being the lateral plan of an embodiment of the present invention.The diameter of bottleneck 2 is R3, which is wide
Bore design, bottleneck diameter R3 are not less than the 1/3 of body maximum gauge R2, and bottom of bottle diameter is R1, and bottom of bottle diameter R1 is generally smaller
In body maximum gauge R2.In a preferred embodiment, bottleneck diameter R3 is not less than the 2/5 of body maximum gauge R2.One
In kind specific embodiment, bottleneck diameter R3 is 100mm, and body maximum gauge R2 is 250mm, and bottom of bottle diameter R1 is 235mm.
As shown in Fig. 2, it is H1 that bottleneck 2, which has certain height, entire shaking flask height is H2, and strip-shaped baffle 31 is by bottom of bottle
Surface bulge, it may have certain height H3, wherein 1mm≤H3≤10mm.In a preferred embodiment, 3mm≤H3≤
8mm.In a kind of specific embodiment, H3 5mm, H1 75mm, H2 275mm.
As shown in figure 3, being the bottom of bottle bottom view of an embodiment of the present invention, there are three items for the tool of bottom of bottle 3 shown in figure
Shape baffle 31, the length of strip-shaped baffle 31 are L, and L is usually the half for being set smaller than flat diameter R1.Three strip-shaped baffles
The center of circle is directed toward by the circumference at round bottle bottom 3 in direction, and bottom of bottle is divided into three fans by three 31 place diametrical directions of strip-shaped baffle
Shape region, the angle theta between three strip-shaped baffles 31 is 120 °.
In other implementations, the number of the strip-shaped baffle 31 of bottom of bottle 3 may be four, five, six etc., more
The center of circle is directed toward by the circumference at round bottle bottom 3 in the direction of a strip-shaped baffle, and bottom of bottle can be divided into multiple by multiple strip-shaped baffles 31
Fan-shaped region, for example, the angle theta between four strip-shaped baffles 31 is 90 °.
The volume specification of high dissolved oxygen glass shaking flask provided by the utility model is preferably 5L, can reach the training of 2L cell
Amount is supported, far more than the cell culture amount of existing shaking flask.
The following are the high dissolved oxygen shaking flasks of the utility model and routine culture shake flat experiment comparing result to compare
1. experimental design:
Material: LB culture medium, IPTG, shaking table, AKTA
Method:
1) it is inoculated with: from plate picking monoclonal bacterial strain, being inoculated into LB of the 200ml with a certain concentration screening resistance antibiotic
In fluid nutrient medium, 30 DEG C, 200rpm is incubated overnight.
2) amplification cultivation: being transferred in the culture shaking flask of design scheme volume for second day, respectively in 5L shaking flask cultivate 1L,
0.5L and 1L is cultivated in 1.4L and 2L, 3L shaking flask.It transfers seed overnight by 1:100,37 cultures grow into OD600 to bacterium is dense
0.6~0.8 induction, the final concentration of 1mM of IPTG, 37 DEG C are continued culture 4 hours, harvest.
3) it purifies: cracking buffer is added by 1:5, cell is resuspended, high-pressure homogenization is broken, centrifugal filtration, loading, purifying.
2. experimental result:
| Shaking flask | Volume of culture | Yield (mg/L) |
| 5L flask | 1L | 94.9 |
| 5L flask | 1.4L | 94.3 |
| 5L flask | 2L | 98.7 |
| 3L flask | 0.5L | 98.0 |
| 3L flask | 1L | 94.2 |
3. interpretation of result
From experimental result it is known that the 5L shaking flask of the utility model design can carry out 2L prokaryotic cell culture, at this
In the case of kind large volume culture, it is normal that dissolved oxygen can achieve cell growth, and protein expression result and conventional shake flask results are always.
4. the popularization and use of shaking flask
Existing shaking flask has been generalized to the use of many projects, expresses for the protokaryon system of various recombinant proteins and antibody.
Nearly 100 multiple protein has been expressed, expression volume has been risen 500, effect is stable always, and cell density is attained by normal bacterium more
It is dense.
In conclusion the various embodiments described above and attached drawing are only the preferred embodiment of the utility model, not to limit
Determine the protection scope of the utility model, within the spirit and principle of the utility model, any modification for being made equally is replaced
It changes, improve, all should include within the protection scope of the present utility model.
Claims (9)
1. a kind of high dissolved oxygen glass shaking flask, including bottleneck, body and bottom of bottle, which is characterized in that the bottleneck is circle, the bottle
Mouth lower end extends downwardly and is gradually expanded the body to form circular cone figure, and the body lower end is formed with circular bottom of bottle, described
Bottom of bottle forms a plurality of strip-shaped baffles of oriented shaking flask internal protrusion；The bottleneck is the design of wide-mouth diameter, and the diameter of bottleneck is not small
In the 1/3 of body maximum gauge；The strip-shaped baffle is by bottom of bottle surface bulge.
2. high dissolved oxygen glass shaking flask as described in claim 1, which is characterized in that a plurality of strip-shaped baffles are respectively positioned on circle
In the different diametrical directions of bottom of bottle, and the angle between two neighboring strip-shaped baffle is equal.
3. high dissolved oxygen glass shaking flask as claimed in claim 2, which is characterized in that the strip-shaped baffle is three, three bar shapeds
The center of circle is directed toward by the circumference at round bottle bottom in the direction of baffle, and the angle between three strip-shaped baffles is 120 °.
4. high dissolved oxygen glass shaking flask as claimed in claim 2, which is characterized in that the strip-shaped baffle is four, four bar shapeds
The center of circle is directed toward by the circumference at round bottle bottom in the direction of baffle, and the angle between four strip-shaped baffles is 90 °.
5. high dissolved oxygen glass shaking flask as described in claim 1, which is characterized in that the height of the strip-shaped baffle is H3, and
0.1mm≤H3≤10mm。
6. high dissolved oxygen glass shaking flask as claimed in claim 5, which is characterized in that 0.2mm≤H3≤1mm.
7. high dissolved oxygen glass shaking flask as described in claim 1, which is characterized in that the diameter of the bottleneck is maximum not less than body
The 2/5 of diameter.
8. high dissolved oxygen glass shaking flask as described in claim 1, which is characterized in that the volume specification of the shaking flask is 5L.
9. such as the described in any item high dissolved oxygen glass shaking flasks of claim 1-8, which is characterized in that the bottleneck diameter is 100mm,
Bottom of bottle diameter is 250mm, and strip-shaped baffle height is 0.5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820515003.2U CN208776740U (en) | 2018-04-11 | 2018-04-11 | High dissolved oxygen glass shaking flask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820515003.2U CN208776740U (en) | 2018-04-11 | 2018-04-11 | High dissolved oxygen glass shaking flask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208776740U true CN208776740U (en) | 2019-04-23 |
Family
ID=66145727
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820515003.2U Active CN208776740U (en) | 2018-04-11 | 2018-04-11 | High dissolved oxygen glass shaking flask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208776740U (en) |
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2018
- 2018-04-11 CN CN201820515003.2U patent/CN208776740U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: Room 2481, Building 1150 Lanfeng Road, Fengxian District, Shanghai, 2010 Patentee after: Shanghai Yaoming Biomedical Co., Ltd. Address before: Room 2481, Building 1150 Lanfeng Road, Fengxian District, Shanghai, 2010 Patentee before: Shanghai Yaoming Kangde Biomedical Co., Ltd. |
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| CP01 | Change in the name or title of a patent holder |