CN206396199U - A kind of Microbiological Culture Collection device - Google Patents
A kind of Microbiological Culture Collection device Download PDFInfo
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- CN206396199U CN206396199U CN201621333024.XU CN201621333024U CN206396199U CN 206396199 U CN206396199 U CN 206396199U CN 201621333024 U CN201621333024 U CN 201621333024U CN 206396199 U CN206396199 U CN 206396199U
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- culture collection
- microbiological culture
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Abstract
The utility model discloses a kind of Microbiological Culture Collection device, it is made up of body and lid with inner chamber, the tubular body bottom is concave inward structure, middle and lower part is provided with microporosity separator in body, set above microporosity separator and discoloration silica gel is set below Microbiological Culture Collection sorbing material, microporosity separator, tube mouth outer wall sets external screw thread, cap wall sets internal thread, external screw thread and screw-internal thread fit；The breather hole cover that sealing air-vent is set on air-vent, air-vent above installation air filtration pad, air-vent is opened up at the top of lid, breather hole cover is fixed in lid by rotary shaft；Transparent label box shell is fixed with tube wall.The Microbiological Culture Collection device that the utility model is provided, takes culture presevation and strain and more facilitates, improve the operating efficiency of culture presevation and the persistence of strain label information, for distinguish preservation whether effectively provide simply, efficiently approach.
Description
Technical field
The utility model is related to a kind of Microbiological Culture Collection device.
Background technology
All strains with merit obtained from production practices or Science Institute, including straight from nature
Connect isolated wild-type strain, and the excellent dissociant or engineering strain selected by manual method etc. is all
It is the important germ plasm resource of country.Therefore, culture presevation is the basis of all microorganisms work, and the purpose of culture presevation is to make bacterium
Kind is not dead after being preserved, do not make a variation, not by living contaminants, and keeps its merit, in favor of producing the application with scientific research.
Under normal circumstances, microorganism is easy to morph, and the metabolic speed of its speed made a variation and cell has
Close, typically when microbial metabolism is vigorous, the speed of mutation of strain is just fast, conversely, metabolic activity is faint, speed of mutation is just slow,
Therefore, could be in a timing in preserving process, it is necessary to make state of the metabolism in least active or geo-stationary of microorganism
Interior it is not morphed and keep activity, low temperature, drying and isolation air are the weights for reducing microbial metabolism ability
Factor is wanted, current culture collection process has kind more than 20, is broadly divided into passage method, suspension method, support methods, boulton process, freezing
Method etc..Wherein support methods are the suitable microorganism adsorption of growth is dried on certain carrier, and this carrier source is very
Extensively, such as soil, sand, silica gel, gelatin, wheat bran, magnetic bead or filter paper.
At present common culture presevation form be test tube, culture dish, sandy soil pipe, lyophilized pipe, ampoul tube etc., wherein test tube,
Culture dish floor space is big, broken, is unfavorable for microorganism dormancy, easily pollutes, be unable to anoxic preservation；Sandy soil Guan Xuansha Sha works
Skill is cumbersome, can not preserve for a long time；Easy cleaning packing, lyophilized efficiency is not low for lyophilized pipe；Ampoul tube is broken, switching easily pollution, one
Pipe is used only once, and storage space-consuming is big, and complex steps when opening.While strain is during long-time is preserved, by
It is easily caused that strain label information outside storage device is fuzzy, stained in the effect of condensed water or external force, loses or damage, because
This current Microbiological Culture Collection device generally existing preparation process is cumbersome, it has not been convenient to operate, bacterium easily invades and polluted, bacterium
Plant information flag and preserve imperfection, be difficult that the problems such as whether fungi preservation fails distinguished.
Utility model content
In view of this, the utility model provides a kind of Microbiological Culture Collection device, culture presevation and strain is taken more
Plus conveniently, the operating efficiency of culture presevation and the persistence of strain label information are improved, to distinguish whether preservation effectively carries
Simple, efficiently approach is supplied.
To solve above technical problem, the technical solution of the utility model provides a kind of Microbiological Culture Collection device,
It is made up of body and lid with inner chamber, and the tubular body bottom is concave inward structure, and body can be protected not damaged by external force
It is bad, improve the security of Microbiological Culture Collection.Middle and lower part is provided with above microporosity separator, microporosity separator and set in the body
Put and discoloration silica gel is set below Microbiological Culture Collection sorbing material, microporosity separator, tube mouth outer wall sets external screw thread, lid
Inwall sets internal thread, external screw thread and screw-internal thread fit；Installation air filtration pad on air-vent, air-vent is opened up at the top of lid,
The breather hole cover of sealing air-vent is set above air-vent, and breather hole cover is fixed in lid by rotary shaft；On tube wall
It is fixed with transparent label box shell.
Further, volume markings tag line is provided with the tube wall, microorganism is weighed for auxiliary operator
Bacteria suspension dispensed loading amount during culture presevation.
Further, the lid outer wall is provided with anti-slip veins, prevents operator from occurring to beat when separating lid and body
It is sliding.
Further, raised line is provided with the breather hole cover, is easy to the rotation of breather hole cover, realize the unlatching of air-vent
And closing.
Further, the lid inner tip is provided with sealed bolster, strengthens the sealing of tube mouth and lid, really
Protect microbial preservation sealing tightly, sealed bolster can be made up of flexible exotic material, such as：Polytetrafluoroethylene (PTFE), silicon rubber
Glue, fluorubber, nitrile rubber, neoprene, chloropharin (ether) rubber, ACM etc..
Further, the Microbiological Culture Collection sorbing material is selected from soil, sand, silica gel, gelatin, wheat bran, magnetic bead
And filter paper.
The utility model by setting microporosity separator in body, its micropore only allow hydrone pass through and other materials very
Hardly possible is passed through, therefore the discoloration silica gel being arranged under microporosity separator can keep in body the drying of local space and to body sealing
Tightly whether, whether Microbiological Culture Collection, which fails, is made instruction, so as to reduce the storage ring of Microbiological Culture Collection device
Border is required, is reduced culture presevation room and is kept xeric equipment and medicine input；In body and lid helixseal, lid
The sealed bolster that top is set strengthens its sealing；Lid sets the air-vent with air filtration pad, it is to avoid Microbiological Culture Collection
Device is in process of vacuum drying, after being particularly dried in vacuo, and the miscellaneous bacteria during pressure release is taken out is invaded, and has ensured microbial bacteria
Kind safety, the utility model device after vacuum drying, rotary closing breather hole cover, can make Microbiological Culture Collection device with
Extraneous air completely cuts off, and has ensured drying, the environment of low oxygen content of Microbiological Culture Collection device.Therefore the utility model is more existing
There is technology to need the technology for filling cotton in pipe to compare, the utility model is dispensed and microorganism in Microbiological Culture Collection sample
Strain, which is taken, more to be facilitated.
The utility model is fixed on the clear label box shell on tube wall, with protection against the tide, waterproof, prevents stained, anti-outer
The functions such as power effect, are pasted onto on tag board in insertion label box shell for recording the label paper of strain information, are prevented effectively from
Microorganism fungus kind preserve for a long time during because causing that strain information flag is fuzzy under condensed water or external force effect, lose, flaw
Dirty or damage phenomenon generation.Label paper by set different colours can be used for observably distinguish conserving microorganism bacterial species,
The every terms of information of Microbiological Culture Collection is can record thereon, and with convertibility, it is both practical and convenient.
In the prior art, cumbersome, time-consuming, the inefficiency of the step of staff prepares Microbiological Culture Collection, therewith
Compare, the utility model provide storage device, its Microbiological Culture Collection sorbing material can prepare with scale, while contain into
In the utility model storage device, unified sterilizing takes when using and used, improves operating efficiency.The utility model can be carried out
Large-scale production, with obvious economic benefit.
It should be noted that make the utility model device material can change, can be transparent or semitransparent glass,
Transparent or semi-transparent high-temperature resistance plastice such as high density polyethylene (HDPE) (HDPE), polypropylene (PP), makrolon (PC), acrylic nitrile-butadiene two
Alkene-styrol copolymer etc..Shape, the size of the utility model device can also change simultaneously, can be in cylinder, four
Prismatic and Else Rule and irregular face shaping.
Brief description of the drawings
Fig. 1 is perspective view of the present utility model；
Fig. 2 is left surface structural representation of the present utility model；
Fig. 3 is right flank structural representation of the present utility model；
Fig. 4 is top view of the present utility model in the case of ventilative bore closure；
Fig. 5 is top view of the present utility model in the case of air-vent is opened.
Marginal data：
1-body；2-lid；3-volume markings tag line；4-label box shell；5-anti-slip veins；6-micropore every
Plate；7-Microbiological Culture Collection sorbing material；8-discoloration silica gel；9-internal thread；10-external screw thread；11-sealed bolster；
12-air-vent；13-air filtration pad；14-breather hole cover；15-rotary shaft；16-raised line.
Embodiment
In order that those skilled in the art more fully understands the technical solution of the utility model, with reference to specific implementation
The utility model is described in further detail for mode.
Referring to Fig. 1 to Fig. 5, the utility model provides a kind of Microbiological Culture Collection device, and it is by the pipe with inner chamber
Body 1 and lid 2 are constituted, referring to Fig. 1, and the inner bottom of body 1 is concave inward structure, and body can be protected not by damage, carried
The high security of Microbiological Culture Collection.Middle and lower part is provided with microporosity separator 6 in the body 1, and the top of microporosity separator 6 is set
Microbiological Culture Collection sorbing material 7, the lower section of microporosity separator 6 sets discoloration silica gel 8, and the mouth of pipe outer wall of body 1 sets external screw thread 10,
The inwall of lid 2 sets internal thread 9, and external screw thread 10 coordinates with internal thread 9；The top of lid 2 is opened up pacifies on air-vent 12, air-vent 12
Air filtration pad 13 is filled, air filtration pad 13 can be the multiple material for possessing filtration sterilization function, and the top of air-vent 12 sets close
The breather hole cover 14 of air-vent is sealed, breather hole cover 14 is fixed in lid 2 by rotary shaft 15；It is fixed with the outer wall of body 1
Bright label box shell 4 and volume markings tag line 3, volume markings tag line 3 is used for auxiliary operator and weighs microorganism fungus kind
Bacteria suspension dispensed loading amount during preservation, label box shell 4 is used to place label paper.
Further, anti-slip veins 5 are provided with the outer wall of lid 2, prevent operator from being skidded in separation lid and body.
Further, raised line 16 is provided with breather hole cover 14, is easy to the rotation of breather hole cover, realize the unlatching of air-vent
And closing.
Further, the inner tip of lid 2 is provided with sealed bolster 11, strengthens the sealing of the mouth of pipe of body 1 and lid 2,
Ensure that microbial preservation sealing is tight, sealed bolster can be made up of flexible exotic material, such as：Polytetrafluoroethylene (PTFE), silicon
Rubber, fluorubber, nitrile rubber, neoprene, chloropharin (ether) rubber, ACM etc..
Further, the Microbiological Culture Collection sorbing material 7 is selected from soil, sand, silica gel, gelatin, wheat bran, magnetic bead
And filter paper.
Microorganism fungus kind is carried out respectively with the utility model device and ampoul tube below to illustrate this reality exemplified by preservation
With new simplicity：
First, comparative example：The preservation of ampoul tube filter paper (quoted from《Microbiology Experiment》Fourth edition chief editor Shen Ping Chen XiangDongs)
(1) preparation of filter paper bar：The small bar that filter paper is cut into 0.5cm × 1.2cm is fitted into 0.6cm × 8cm ampoul tube,
Every pipe dress 1-2 pieces, with after on cotton plug through 121 DEG C of sterilizing 30min.
(2) protectant configuration：20% defatted milk is configured, in conical flask or test tube, 112 DEG C of sterilizing 25min.Treat
Several parts are taken out after cooling at random and puts 28 DEG C, 37 DEG C of overnight incubations respectively, then respectively takes 0.2ml to be coated on meat soup flat board or inclined-plane
Upper carry out sterility test, confirms that sterile rear can be used, it is stand-by that remaining protective agent puts 4 DEG C of storages.
(3) Spawn incubation：The strain of preservation is cultivated on suitable slant medium, until its growth is plentiful.Bacterium is hanged
The preparation of liquid：Take sterile absorbent milk 2-3ml to add in strain inclined plane test tube to be saved, lightly scraped lawn with oese
Under, through oscillator shaken well, as bacteria suspension.
(4) sample is dispensed：Bacteria suspension is drawn with sterile dropper (or suction pipe) to drop on the filter paper bar in ampoul tube, every filter
Paper slip about drips 0.5ml, beyond the Great Wall cotton.
(5) dry：Ampoul tube is put into phosphorus pentoxide (or anhydrous calcium chloride) to make in the drier of water absorbing agent, with true
Empty pumping its to dry.
(6) sealing is with preserving：With flame by ampoule channel closure, deposited to 4 DEG C or room temperature.
(7) ampoul tube is taken：During using strain, the ampoul tube file or emery wheel of storage is taken to be drawn in ampoul tube upper end tube wall
Trace, then breaks into two with one's hands or by the calcination on alcolhol burner flame at the top of ampoul tube, the rapid upper cold sterilized water of drop fries ampoul tube front end
Open, knock out the glass of upper end, take out filter paper with aseptic nipper, be put into fluid nutrient medium or a little sterilized water aseptic straw or hair
Tubule is blown and beaten several times, dried object is suctioned out after dissolving quickly, is transferred in appropriate culture medium and cultivates.
This method exist shortcoming be：
(1) cotton is filled in ampoul tube, sample packing process needs sterile working, bacteria suspension is added then after cotton is taken out
Cotton is filled in again, has not only been not easy to operation but also easily microbiological contamination in operation.
(2) vavuum pump is drained in moisture process because ampoul tube can not be fully sealed, and easily causes miscellaneous bacteria intrusion.
(3) because the method microorganism fungus kind holding time is long, easily because being led under condensed water or external force effect during the preservation
Cause fuzzy, stained strain information flag, loss or damage.
(4) ampoul tube sealing with strain in-convenience in use, one is in ampoul tube upper end tube wall cut, so with file or emery wheel
After break into two with one's hands, two be that, by the calcination on alcolhol burner flame at the top of ampoul tube, the rapid upper cold sterilized water of drop blows ampoul tube front end,
User is easily caused by ampoul tube glass scratch or scald, is unfavorable for ensureing the health and safety of human body.
(5) microorganism fungus kind preserves whether pipe fails, and sealing whether there is problem, is difficult to distinguish.
2nd, embodiment：Preserved with the utility model device filter paper
(1) preparation of filter paper bar：The small bar that filter paper is cut into 0.5cm × 1.2cm loads the utility model device microorganism
In culture presevation pipe, often pipe dress 1-2 pieces, are covered tightly after lid through 121 DEG C of sterilizing 30min.
(2) protectant configuration：20% defatted milk is configured, in conical flask or test tube, 112 DEG C of sterilizing 25min.Treat
Several parts are taken out after cooling at random and puts 28 DEG C, 37 DEG C of overnight incubations respectively, then respectively takes 0.2ml to be coated on meat soup flat board or inclined-plane
Upper carry out sterility test, confirms that sterile rear can be used, it is stand-by that remaining protective agent puts 4 DEG C of storages.
(3) Spawn incubation：The strain of preservation is cultivated on suitable slant medium, until its growth is plentiful.
(4) preparation of bacteria suspension：Take sterile absorbent milk 2-3ml to add in strain inclined plane test tube to be saved, use oese
Lightly lawn is scraped, through oscillator shaken well, as bacteria suspension.
(5) sample is dispensed：Bacteria suspension, which is drawn, with sterile dropper (or suction pipe) drops in the utility model device microorganism fungus kind
On filter paper bar in preservation pipe, every filter paper treaty drips 0.5ml, lid is covered tightly, by Microbiological Culture Collection tube wall label box
Label paper in shell is taken out with tag board from label box shell, and the every terms of information of preservation of bacteria strain is recorded on label paper
Afterwards, label paper is put back in label box shell with tag board.
(6) dry：Unscrew breather hole cover, phosphorus pentoxide (or anhydrous chlorine has been put into by Microbiological Culture Collection pipe
Change calcium) make in the drier of water absorbing agent, with vacuum pumping, it is extremely dry.
(7) preserve：Rotary closing breather hole cover, is deposited to 4 DEG C or room temperature.
(8) take：During using strain, Microbiological Culture Collection pipe lid is opened, filter paper is taken out with aseptic nipper, is put into liquid
Body culture medium or the piping and druming of a little sterilized water aseptic straw or capillary several times, make dried object be suctioned out after dissolving quickly, are transferred to suitable
When culture medium in cultivate.
The advantage of this method is：
(1) take culture presevation and strain more to facilitate, reduce injuring unexpectedly for staff, improve support methods
The operating efficiency of conserving microorganism bacterial；
(2) persistence of support methods conserving microorganism bacterial mark and the security of strain are improved；
(3) for identification carrier method conserving microorganism bacterial whether effectively provide simply, efficiently approach.
It the above is only preferred embodiment of the present utility model, it is noted that above-mentioned preferred embodiment should not be regarded
For to limitation of the present utility model, protection domain of the present utility model should be defined by claim limited range.For
For those skilled in the art, do not departing from spirit and scope of the present utility model, can also make some
Improvements and modifications, these improvements and modifications also should be regarded as protection domain of the present utility model.
Claims (6)
1. a kind of Microbiological Culture Collection device, is made up of body and lid with inner chamber, it is characterised in that：In the body
Portion bottom is that middle and lower part is provided with setting Microbiological Culture Collection suction above microporosity separator, microporosity separator in concave inward structure, body
Discoloration silica gel is set below enclosure material, microporosity separator, and tube mouth outer wall sets external screw thread, and cap wall sets internal thread, outside
Screw thread and screw-internal thread fit；Opened up at the top of lid and sealing is set on air-vent, air-vent above installation air filtration pad, air-vent
The breather hole cover of air-vent, breather hole cover is fixed in lid by rotary shaft；Transparent label box is fixed with tube wall
Shell.
2. a kind of Microbiological Culture Collection device according to claim 1, it is characterised in that：Set on the tube wall
There is volume markings tag line.
3. a kind of Microbiological Culture Collection device according to claim 1, it is characterised in that：The lid outer wall is provided with
Anti-slip veins.
4. a kind of Microbiological Culture Collection device according to claim 1, it is characterised in that：Set on the breather hole cover
There is raised line.
5. a kind of Microbiological Culture Collection device according to claim 1, it is characterised in that：The lid inner tip is set
It is equipped with sealed bolster.
6. a kind of Microbiological Culture Collection device according to claim 1, it is characterised in that：The Microbiological Culture Collection
Sorbing material is selected from soil, sand, silica gel, gelatin, wheat bran, magnetic bead and filter paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621333024.XU CN206396199U (en) | 2016-12-06 | 2016-12-06 | A kind of Microbiological Culture Collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621333024.XU CN206396199U (en) | 2016-12-06 | 2016-12-06 | A kind of Microbiological Culture Collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206396199U true CN206396199U (en) | 2017-08-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201621333024.XU Active CN206396199U (en) | 2016-12-06 | 2016-12-06 | A kind of Microbiological Culture Collection device |
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| CN (1) | CN206396199U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108660066A (en) * | 2018-05-24 | 2018-10-16 | 黄河科技学院 | Preclinical medicine pathogen culture ware |
| CN112899166A (en) * | 2021-04-06 | 2021-06-04 | 华中农业大学 | Fungus strain preservation method |
-
2016
- 2016-12-06 CN CN201621333024.XU patent/CN206396199U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108660066A (en) * | 2018-05-24 | 2018-10-16 | 黄河科技学院 | Preclinical medicine pathogen culture ware |
| CN112899166A (en) * | 2021-04-06 | 2021-06-04 | 华中农业大学 | Fungus strain preservation method |
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| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200102 Address after: 641409 Pingquan Town, Jianyang City, Chengdu City, Sichuan Province Patentee after: Sichuan Runer Technology Co., Ltd Address before: 641404, 80 chessboard Road, Chengdu, Sichuan, Jianyang Patentee before: Sichuan Guoguang Agrochemical Co., Ltd. |
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| TR01 | Transfer of patent right |