CN109456892A - A kind of incubator of accelerated cell growth rate - Google Patents
A kind of incubator of accelerated cell growth rate Download PDFInfo
- Publication number
- CN109456892A CN109456892A CN201811518463.1A CN201811518463A CN109456892A CN 109456892 A CN109456892 A CN 109456892A CN 201811518463 A CN201811518463 A CN 201811518463A CN 109456892 A CN109456892 A CN 109456892A
- Authority
- CN
- China
- Prior art keywords
- culture dish
- ware
- dish unit
- chassis
- incubator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000010261 cell growth Effects 0.000 title claims abstract description 35
- 239000007787 solid Substances 0.000 claims description 24
- 230000004888 barrier function Effects 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 14
- 238000007789 sealing Methods 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 11
- 235000015097 nutrients Nutrition 0.000 claims description 11
- 239000002699 waste material Substances 0.000 claims description 7
- 238000012549 training Methods 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 5
- 240000001131 Nostoc commune Species 0.000 abstract description 3
- 235000013817 Nostoc commune Nutrition 0.000 abstract description 3
- 238000004113 cell culture Methods 0.000 description 10
- 239000002609 medium Substances 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 8
- 238000009472 formulation Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 238000009630 liquid culture Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000010876 biochemical test Methods 0.000 description 2
- 229920001971 elastomer Polymers 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 230000000873 masking effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000004308 accommodation Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to Bioexperiment equipment technical fields, more particularly to a kind of incubator of accelerated cell growth rate, including the chassis set gradually from bottom to top and several culture dish units, it is rotatably connected on same connecting column between the chassis and culture dish unit, and the culture dish unit can be moved up and down along the connecting column, the culture dish unit small big boss shape structure under being, the i.e. described culture dish unit includes the biggish ware body of outer diameter and the lesser ware bottom of outer diameter, it can match and connect together between the ware body and ware bottom of adjacent culture dish unit, by different culture dish unit stacked in multi-layers, it can match and be socketed in the chassis positioned at the ware bottom of the culture dish unit of the bottom.The present invention plays multiple firm effect by culture dish unit, connecting column and the chassis of setting boss shape structure, can be effectively prevented multiple Nostoc commune Vanch ware heap poststacks be easy it is at random or the problem of overturn.
Description
Technical field
The invention belongs to Bioexperiment equipment technical fields, and in particular to a kind of incubator of accelerated cell growth rate.
Background technique
Cell is one of essential material of biochemical test, cell culture be then carry out biochemical test necessary means it
One, for accelerated cell growth, different cells needs different dedicated liquid culture solutions.Due to not knowing for Bioexperiment
Property, it may be necessary to multiplicating experiment is done, the cell culture time is longer in addition, therefore experimental implementation person can generally cultivate largely
Cell is spare.The vessel that prior art cell culture uses are culture dish or culture bottle.Culture bottle volume is big, and height is high, right
Culture device accommodation space requires height, therefore the use of culture dish is more extensive.Culture dish diameter range is limited, a culture dish
The cell quantity that can be cultivated is also limited.If necessary to cultivate a large amount of cell, then need using to multiple culture dishes, Duo Gepei
It supports ware heap poststack and concentrates culture in the incubator again, fugitive batter of the culture dish directly stacked is turned over, and stability is bad.The prior art
Also have and multiple culture dishes are stacked and are cultivated with being concentrated in the incubator again after the package such as masking foil, but wrapped up with masking foil etc.
Before, Tissue Culture Dish is also that simple heap is folded, and has no additional fixation device, will also result at random in encapsulation process or overturn
The problem of.
To sum up, problem of the existing technology is that the culture dish that the prior art is used to cultivate cell lacks fixed device, more
A culture dish heap poststack is easy at random or overturns.
Summary of the invention
Of the invention provides a kind of incubator of accelerated cell growth rate, solves the prior art for cultivating cell
Culture dish lacks fixed device, and multiple culture dish heap poststacks are easy at random or the problem of overturn.The stacked placement of the present invention it is more
Different formulations fluid nutrient medium can be held in a culture dish, to adapt to the cell of different stages of growth, pass through different formulations liquid
Culture medium is applied in combination to improve cell growth rate；The different formulations culture medium that a kind of cell culture uses is placed in a tissue culture
It supports in device, the cell of different phase growth can be quickly obtained.
A kind of incubator of accelerated cell growth rate provided by the invention, including the chassis that sets gradually from bottom to top and
Several culture dish units, are rotatably connected on same connecting column between the chassis and culture dish unit, and the training
Supporting ware unit can move up and down along the connecting column, the culture dish unit small big boss shape structure, i.e., described training under being
Supporting ware unit includes the biggish ware body of outer diameter and the lesser ware bottom of outer diameter, energy between the ware body and ware bottom of adjacent culture dish unit
With connecting together, by different culture dish unit stacked in multi-layers, the ware bottom positioned at the culture dish unit of the bottom can match set
It is connected in the chassis.
Preferably, in the incubator of above-mentioned accelerated cell growth rate, the ware body is located at the position on the outside of the ware bottom
It is set to ware body solid part, the ware body solid part of the ware body offers longitudinal ware body jack hole, and the chassis, which is equipped with, to be made
The connecting column wears the chassis solid part connect, and the chassis opposite with the ware body jack hole is offered in the chassis solid part and is worn
Connect hole, the connecting column sequentially passes through all ware body jack hole and chassis jack hole, and by the chassis and culture dish unit
It is rotatably connected.
Preferably, in the incubator of above-mentioned accelerated cell growth rate, the connecting column bottom end is fixed on shown chassis
Chassis solid part on, form a L-shaped brackets.
Preferably, in the incubator of above-mentioned accelerated cell growth rate, the chassis is identical as the ware shape,
For cylindrical shape.
Preferably, in the incubator of above-mentioned accelerated cell growth rate, the upper end of the connecting column, positioned at top
Operating handle is removably arranged with above the culture dish unit.
Preferably, in the incubator of above-mentioned accelerated cell growth rate, between the adjacent culture dish unit, the bottom
Culture dish unit and chassis between be provided with sealing ring, and the sealing ring is set in outside the ware bottom of the culture dish unit
Portion.
Preferably, in the incubator of above-mentioned accelerated cell growth rate, the sealing ring includes one openable soft
Washer and the raised line being arranged along the soft washer inner sidewall length direction, the raised line can be embedded in the ware of adjacent culture dish unit
In gap between body or the gap that can be embedded between ware body and chassis, the soft washer can be by adjacent culture dish unit
Gap package between gap or ware body and chassis between ware body, the both ends of the soft washer are detachably connected by connector
It is connected together.
Preferably, in the incubator of above-mentioned accelerated cell growth rate, positioned at the culture dish unit of top
Culture ware lid is covered on ware body.
Preferably, in the incubator of above-mentioned accelerated cell growth rate, the ware bottom bottom of the culture dish unit is opened up
There is discharge orifice, barriers is inlaid in ware underflow water hole, and the barriers allow liquid reagent or fluid nutrient medium logical
It crosses, does not allow cell to pass through.
Preferably, the incubator of above-mentioned accelerated cell growth rate further includes waste liquid cylinder, the chassis, culture dish unit
It is placed in waste liquid cylinder with connecting column.
Compared with prior art, the incubator of a kind of accelerated cell growth rate of the invention, has the advantages that
1, the present invention plays multiple firm effect by culture dish unit, connecting column and the chassis of setting boss shape structure,
Can be effectively prevented multiple Nostoc commune Vanch ware heap poststacks be easy it is at random or the problem of overturn.Different culture dish units are also in the present invention
It can rotate to different location, inoculating cell is facilitated to work, achieve the purpose that layering culture, thus accelerated cell growth and primary
Property can cultivate the total amount of cell.The device of the invention can move up and down different culture dish units along connecting column, in order to
Take out the cell culture in different culture dishes.
2, in order to further speed up the growth rate of cell, cell culture different phase can be trained using the liquid of different formulations
Base is supported, the present invention is applied in combination to improve cell growth rate by different formulations fluid nutrient medium, and cell is avoided entirely to grow
Stage is all made of single culture medium.Compared with the culture bottle for commonly toppling over method replacement culture medium, the present invention passes through at ware bottom bottom
Portion offers discharge orifice and setting barriers, can accelerate the speed for replacing different fluid nutrient mediums, improves culture medium replacement effect
Rate.
Detailed description of the invention
Structural schematic diagram when Fig. 1 is the incubator expansion of accelerated cell growth rate of the present invention；
Structural schematic diagram when Fig. 2 is the incubator stacking of accelerated cell growth rate of the present invention；
Fig. 3 is structural schematic diagram when sealing ring is unfolded after adjacent culture dish unit connects；
Fig. 4 is structural schematic diagram when sealing ring is closed after adjacent culture dish unit connects.
Description of symbols:
1. chassis, 2. ware bodies, 21. ware bottoms, 211. solid parts, 212. ware body jack holes, 22. barriers, 3. connecting columns,
31. operating handle, 4. culture ware lids, 5. soft washers, 51. raised lines, 52. connectors.
Specific embodiment
With reference to the accompanying drawing, specific embodiments of the present invention will be described in detail, it is to be understood that guarantor of the invention
Shield range is not limited by the specific implementation.
A kind of incubator of accelerated cell growth rate, it is specific as shown in Figs 1-4, including the bottom set gradually from bottom to top
Disk 1 and 2-4 culture dish unit, are rotatably connected on same connecting column 3 between chassis 1 and culture dish unit, and train
Support ware unit can be moved up and down along connecting column 3, culture dish unit under being small big boss shape structure, i.e. culture dish unit include
The biggish ware body 2 of outer diameter and the lesser ware bottom 21 of outer diameter, ware body 2 are connected to ware bottom 21, the ware body 2 and ware of adjacent culture dish unit
It can match and connect together between bottom 21, and then by different culture dish unit stacked in multi-layers, positioned at the culture dish list of the bottom
The ware bottom 21 of member, which can match, to be socketed in chassis 1；In the present invention, connect to stablize different culture dish units and connecting column 3
It connects, the position that ware body 2 is located at 21 outside of ware bottom is ware body solid part 211, and the ware body solid part 211 of ware body 2 offers longitudinal
Ware body jack hole 212, chassis 1, which is equipped with, can make connecting column 3 wear the chassis solid part connect, offer in the solid part of chassis and ware body
The opposite chassis jack hole of jack hole 212, connecting column 3 sequentially pass through all ware body jack hole 212 and chassis jack hole, and will
Chassis 1 and culture dish unit rotational link together.Note: ware body jack hole 212 is not connected to 2 inner space of ware body, chassis
Jack hole is not connected to 1 inner space of chassis, and ware body solid part 211, chassis solid part only connect the use of connecting column 3 for wearing.
In the present invention, culture dish unit is for placing culture solution, solid medium etc. to cultivate cell, the culture of top
Ware unit serves as the effect of lid, plays dust-proof Deng protective effects；Due to culture dish unit be both connected on connecting column 3, then
Culture dish unit is transversely movable to be limited in scope, and moves in a circle mainly around connecting column 3, connecting column 3 can play linking and
The effect of firm culture dish unit；Once stacking is got up between the culture dish unit of boss shape structure, culture dish unit is difficult to a left side
Right shaking, therefore boss shape structure also has the function of firm culture dish unit；Play load-bearing and firm dual work in chassis 1
With.Therefore, the device of the invention can be effectively prevented multiple Nostoc commune Vanch ware heap poststacks be easy it is at random or the problem of overturn.
Different culture dish units can also be rotated to different location in the present invention, as shown in Figure 1, facilitating inoculating cell work
Make, achieve the purpose that layering culture, to accelerated cell growth and can disposably cultivate the total amount of cell.The device of the invention can
Different culture dish units is moved up and down along connecting column 3, to take out the cell culture in different culture dishes.
For the ease of producing and saving, chassis 1 is identical as 2 shape of ware body, is cylindrical shape, and the two has identical knot
The solid part of structure, and 1 bottom of chassis be it is solid, chassis 1 can also be used to culture cell.
3 bottom end of connecting column is fixed on the chassis solid part on chassis 1 by screw connection manner, 3 bottom end of connecting column also can weld or
Person's welding forms a L-shaped brackets in the chassis solid part on chassis 1, and multiple culture dish units are made, stability is more
It is good.
The upper end of connecting column 3 is removably arranged with operating handle 31 above the culture dish unit of top, behaviour
Make handle 31 to be spirally connected and be set in 3 top of connecting column, has carried whole device using operating handle 31, be moved easily device.
In the present invention, culture ware lid 4 is covered on the ware body 2 of the culture dish unit of the bottom.It cultivates in ware lid 4
Being provided with allows connecting column 3 to wear the ware lid jack hole connect, and culture 4 inner roof wall of ware lid can be pasted with the ware body solid part 211 of ware body 2
It closes, ware lid jack hole is located at 211 top of ware body solid part, can cover conjunction ware body 2 well in order to cultivate ware lid 4.
The substances such as liquid in moving process in different culture dish units overflow in order to prevent, we are in adjacent culture dish list
Sealing ring is also provided between member, between the culture dish unit and chassis 1 of the bottom.Sealing ring be rubber or latex circle,
And it is set in outside the ware bottom 21 of culture dish unit；Or sealing ring is including an openable soft washer 5 and along soft washer 5
The raised line 51 of inner sidewall length direction setting, raised line 51 can be embedded between the ware body 2 of adjacent culture dish unit or the bottom
In gap between culture dish unit and chassis 1, soft washer 5 can be by between the ware body 2 of adjacent culture dish unit or most bottom
Gap between the culture dish unit and chassis 1 of layer is wrapped up, and the both ends end of soft washer 5 passes through button, snap-fastener or velcro
Equal connectors 52 are detachably connected.Soft washer 5 and raised line 51 are integrally formed, using rubber, latex or other can rise
To the material of sealing function.After 5 both ends of soft washer link together, the both ends of raised line 51 form an annulus shape just.
In the present invention, 21 bottom of ware bottom of culture dish unit offers discharge orifice, and ware bottom is inlaid with resistance in 21 discharge orifice
Spacing body 22, and barriers 22 allow to pass through containing hydrone, the liquid reagent of ion or fluid nutrient medium, do not allow cell logical
It crosses, such as the filter screen that barriers 22 are 40 μm, 70 μm or 100 μm of mesh diameter etc..
In order to further speed up the growth of cell, cell culture different phase can use the fluid nutrient medium of different formulations,
Culture dish unit positioned at top carries out cell culture using the structure with barriers 22, remaining different culture dish unit is adopted
With the bottom no leakage structure without barriers 22, and remaining different culture dish unit is put into the Liquid Culture of different formulations
The culture dish unit ware bottom 21 of base, top enters in adjacent culture dish unit liquid culture medium below, starts cell training
It supports；When needing replacing culture medium, without toppling over operation, it is only necessary to move up culture dish list containing cell along connecting column 3
Member, the liquid in the culture dish unit after having flowed, remove unused along the culture dish unit that barriers 22 flow into below
The new of new culture dish unit is immersed in culture dish unit ware bottom 21 containing cell and with barriers 22 by culture dish unit again
In culture medium, due to the presence of barriers 22, operation bring loss cell is toppled in reduction.
Based on the above-mentioned culture dish cellular construction with barriers 22, there are also another usage modes by the present invention: all trainings
It supports ware unit and is all made of the structure containing barriers 22, be laminated in the way of Fig. 2, using sealing ring by adjacent culture dish unit
And the gap sealing between chassis, prevent leakage；Each culture dish unit can cultivate cell, first from top layer's culture dish list
Member injection fluid nutrient medium, finally flows into chassis 1, as food steamer；When needing replacing liquid, all cultures are moved up
Ware unit, then the fluid nutrient medium in these culture dish units is flowed out from respective barriers 22, complete to Liquid Culture base flow
Afterwards, fluid nutrient medium in chassis 1 is finally outwelled, the culture solution more renewed, and as each component, continuation cell is laminated in the structure of Fig. 2
Culture operation；So, it can be achieved that the stacked culture of cell high-volume.
In order to facilitate waste liquid is collected, the device of the invention further includes waste liquid cylinder, first by chassis 1, culture dish unit and connection
Column 3 is placed in waste liquid cylinder, is then carrying out discharge fluid nutrient medium operation.
It should be noted that repeating in order to prevent, the present invention describes preferred embodiment, but technology people in the art
Member is once know basic creative concept, then additional changes and modifications can be made to these embodiments.So appended right
It is required that being intended to be construed to includes preferred embodiment and all change and modification for falling into the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (10)
1. a kind of incubator of accelerated cell growth rate, which is characterized in that including the chassis (1) that sets gradually from bottom to top and
Several culture dish units are rotatably connected on same connecting column (3) between the chassis (1) and culture dish unit, and
The culture dish unit can be moved up and down along the connecting column (3), the culture dish unit small big boss shape structure under being,
The i.e. described culture dish unit includes the biggish ware body (2) of outer diameter and the lesser ware bottom (21) of outer diameter, the ware of adjacent culture dish unit
It can match and connect together between body (2) and ware bottom (21), by different culture dish unit stacked in multi-layers, positioned at the training of the bottom
The ware bottom (21) for supporting ware unit, which can match, to be socketed in the chassis (1).
2. the incubator of accelerated cell growth rate according to claim 1, which is characterized in that the ware body (2) is located at
Position on the outside of the ware bottom (21) is ware body solid part (211), and the ware body solid part (211) of the ware body (2) offers vertical
To ware body jack hole (212), the chassis (1) be equipped with the connecting column (3) can be made to wear the chassis solid part connect, the bottom
The chassis jack hole opposite with the ware body jack hole (212) is offered in disk solid part, the connecting column (3) sequentially passes through institute
Some ware body jack holes (212) and chassis jack hole, and the chassis (1) and culture dish unit rotational are linked together.
3. the incubator of accelerated cell growth rate according to claim 2, which is characterized in that connecting column (3) bottom
End is fixed in the chassis solid part on shown chassis (1), forms a L-shaped brackets.
4. the incubator of accelerated cell growth rate according to claim 2, which is characterized in that the chassis (1) and institute
It is identical to state ware body (2) shape, is cylindrical shape.
5. the incubator of accelerated cell growth rate according to claim 1, which is characterized in that the connecting column (3)
Upper end is removably arranged with operating handle (31) above the culture dish unit of top.
6. the incubator of accelerated cell growth rate according to claim 1, which is characterized in that the adjacent culture dish list
It is provided with sealing ring between member, between the culture dish unit of the bottom and chassis (1), and the sealing ring is set in the training
The ware bottom (21) for supporting ware unit is external.
7. the incubator of accelerated cell growth rate according to claim 6, which is characterized in that the sealing ring includes one
A openable soft washer (5) and the raised line (51) being arranged along the soft washer (5) inner sidewall length direction, the raised line
(51) gap that can be embedded between the ware body (2) of adjacent culture dish unit or the seam that can be embedded between ware body (2) and chassis (1)
In gap, the soft washer (5) can be by the gap or ware body (2) and chassis (1) between the ware body (2) of adjacent culture dish unit
Between gap package, the both ends of the soft washer (5) are detachably connected by connector (52).
8. the incubator of accelerated cell growth rate according to claim 1, which is characterized in that described in top
Culture ware lid (4) is covered on the ware body (2) of culture dish unit.
9. the incubator of accelerated cell growth rate according to claim 1, which is characterized in that the culture dish unit
Ware bottom (21) bottom offers discharge orifice, is inlaid with barriers (22) in ware bottom (21) discharge orifice, and the barriers
(22) allow liquid reagent or fluid nutrient medium to pass through, do not allow cell to pass through.
10. the incubator of accelerated cell growth rate according to claim 9, which is characterized in that further include waste liquid cylinder, institute
Chassis (1), culture dish unit and connecting column (3) is stated to be placed in waste liquid cylinder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811518463.1A CN109456892A (en) | 2018-12-12 | 2018-12-12 | A kind of incubator of accelerated cell growth rate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811518463.1A CN109456892A (en) | 2018-12-12 | 2018-12-12 | A kind of incubator of accelerated cell growth rate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109456892A true CN109456892A (en) | 2019-03-12 |
Family
ID=65613175
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811518463.1A Pending CN109456892A (en) | 2018-12-12 | 2018-12-12 | A kind of incubator of accelerated cell growth rate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109456892A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110564599A (en) * | 2019-09-24 | 2019-12-13 | 西安市第四医院 | Anti-pollution formula bacteria culture device of clinical laboratory |
| CN112143648A (en) * | 2020-09-30 | 2020-12-29 | 四川省畜牧科学研究院 | Poultry cell culture device and poultry cell culture method |
| CN116987591A (en) * | 2023-07-13 | 2023-11-03 | 张北国瑞医疗器械有限公司 | Stem cell culture dish piles up equipment |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010042072A1 (en) * | 2008-10-08 | 2010-04-15 | Agency For Science, Technology And Research | Apparatus for culturing anchorage dependent cells |
| JP2010142143A (en) * | 2008-12-17 | 2010-07-01 | Sanyo Electric Co Ltd | Cell culture container and cell culture apparatus |
| CN105132286A (en) * | 2009-07-22 | 2015-12-09 | 康宁有限公司 | Multilayer tissue culture vessel |
| WO2016069892A1 (en) * | 2014-10-29 | 2016-05-06 | Corning Incorporated | Devices and methods for generation and culture of 3d cell aggregates |
| JP2016103984A (en) * | 2014-12-01 | 2016-06-09 | 株式会社日立製作所 | Stack type culture vessel |
| CN205653466U (en) * | 2015-12-31 | 2016-10-19 | 深圳市第二人民医院 | Porous tissue culture dish structure |
| CN206408248U (en) * | 2016-12-30 | 2017-08-15 | 福建泽源生物科技有限公司 | A kind of culture dish |
| CN208151368U (en) * | 2018-04-03 | 2018-11-27 | 张歆 | A kind of biological culture dish |
-
2018
- 2018-12-12 CN CN201811518463.1A patent/CN109456892A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010042072A1 (en) * | 2008-10-08 | 2010-04-15 | Agency For Science, Technology And Research | Apparatus for culturing anchorage dependent cells |
| JP2010142143A (en) * | 2008-12-17 | 2010-07-01 | Sanyo Electric Co Ltd | Cell culture container and cell culture apparatus |
| CN105132286A (en) * | 2009-07-22 | 2015-12-09 | 康宁有限公司 | Multilayer tissue culture vessel |
| WO2016069892A1 (en) * | 2014-10-29 | 2016-05-06 | Corning Incorporated | Devices and methods for generation and culture of 3d cell aggregates |
| JP2016103984A (en) * | 2014-12-01 | 2016-06-09 | 株式会社日立製作所 | Stack type culture vessel |
| CN205653466U (en) * | 2015-12-31 | 2016-10-19 | 深圳市第二人民医院 | Porous tissue culture dish structure |
| CN206408248U (en) * | 2016-12-30 | 2017-08-15 | 福建泽源生物科技有限公司 | A kind of culture dish |
| CN208151368U (en) * | 2018-04-03 | 2018-11-27 | 张歆 | A kind of biological culture dish |
Non-Patent Citations (2)
| Title |
|---|
| AZADEH NILGHAZ ET AL.: "Multilayer cell culture system supported by thread", 《SENSORS AND ACTUATORS B: CHEMICAL》 * |
| 施中凯 等: "一种新型检验科用培养皿架的制作与应用", 《世界最新医学信息文摘》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110564599A (en) * | 2019-09-24 | 2019-12-13 | 西安市第四医院 | Anti-pollution formula bacteria culture device of clinical laboratory |
| CN110564599B (en) * | 2019-09-24 | 2023-01-03 | 西安市第四医院 | Anti-pollution formula bacteria culture device of clinical laboratory |
| CN112143648A (en) * | 2020-09-30 | 2020-12-29 | 四川省畜牧科学研究院 | Poultry cell culture device and poultry cell culture method |
| CN116987591A (en) * | 2023-07-13 | 2023-11-03 | 张北国瑞医疗器械有限公司 | Stem cell culture dish piles up equipment |
| CN116987591B (en) * | 2023-07-13 | 2024-03-29 | 青岛大学附属医院 | Stem cell culture dish piles up equipment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109456892A (en) | A kind of incubator of accelerated cell growth rate | |
| CN105132286B (en) | Multilayer cell culture vessel | |
| CN109868256A (en) | A kind of kidney organ's chip and kidney organ's drug test model | |
| CN101549179B (en) | Perforated brick type filling support type reactor used in artificial liver | |
| JP6588845B2 (en) | Cell culture vessel and jig for fixing cell culture vessel | |
| CN207560911U (en) | A kind of seedlings nursing plate convenient for culturing and transplanting seedlings | |
| CN206559906U (en) | A kind of changeable soles water planting field planting basket | |
| CN115651841B (en) | Device and method for co-culturing multiple cells of retina | |
| CN207235560U (en) | Flowerpot watering save-all | |
| CN206533794U (en) | A kind of blueberry planting unit | |
| CN206945388U (en) | Sample uniform filtration box | |
| CN106665169A (en) | Flowerpot facilitating transplanting | |
| CN209668897U (en) | A kind of complex intensifying to purify water is biological floating bed | |
| CN209405361U (en) | A kind of waste liquid drainage device for CRRT treatment | |
| CN206831150U (en) | Liquid nitrogen canister assembly | |
| CN207443828U (en) | A kind of water planting container | |
| CN112625881A (en) | Negative pressure culture apparatus convenient to collagen draws | |
| CN219099195U (en) | A kind of layered cell culture bottle which is convenient for replenishment and replacement | |
| CN212728374U (en) | Movable flower tissue culture operation table | |
| CN206932884U (en) | A kind of vegetable fertilizer device for supplying | |
| CN207202507U (en) | New green environment protection bookshelf | |
| CN214881581U (en) | There is not zona pellucida embryo culture apparatus | |
| CN207175930U (en) | A kind of multi-functional photosynthetic microbial cultivation device | |
| CN214508295U (en) | Artificial planting and culturing device for south medicine plants | |
| CN207498360U (en) | A kind of rhizobium enrichment culture device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190312 |
|
| RJ01 | Rejection of invention patent application after publication |