CN112143648A

CN112143648A - Poultry cell culture device and poultry cell culture method

Info

Publication number: CN112143648A
Application number: CN202011057652.0A
Authority: CN
Inventors: 张增荣; 蒋小松; 杜华锐; 杨朝武; 李晴云; 邱莫寒; 余春林; 彭涵; 熊霞; 杨礼; 胡陈明; 夏波; 宋小燕; 陈家磊
Original assignee: SICHUAN ANIMAL HUSBANDRY SCIENCE RESEARCH INSTITUTE
Current assignee: SICHUAN ANIMAL HUSBANDRY SCIENCE RESEARCH INSTITUTE
Priority date: 2020-09-30
Filing date: 2020-09-30
Publication date: 2020-12-29

Abstract

A poultry cell culture device and a poultry cell culture method relate to the field of poultry biotechnology. The culture device comprises a base, a support plate, a first culture dish, a second culture dish, a third culture dish, a transmission gear and a driving rack. Two backup pads are parallel, the interval sets up and all install in the base. First culture dish, second culture dish and third culture dish all locate between two backup pads and range upon range of setting, and first culture dish has first rack, and the third culture dish has the second rack. The transmission gear is rotatably arranged on the supporting plate, and the first rack and the second rack are meshed with the transmission gear. The driving rack is matched with the supporting plate in a sliding mode and meshed with the transmission gear. It can provide enough big culture space and enough quantity culture unit, and it is very convenient to fetch, shift, allotment, and the security is high. The culture method is not easy to pollute the culture environment, is very convenient to take, transfer and allocate, has high safety, and is beneficial to further improving the culture quality and the culture efficiency.

Description

Poultry cell culture device and poultry cell culture method

Technical Field

The invention relates to the field of poultry biotechnology, in particular to a poultry cell culture device and a poultry cell culture method.

Background

In the poultry cell culture work, the culture amount is generally larger, and the amount of the culture medium used is also generally larger. The process of using the existing equipment for culture occupies a large space, and the processes of taking, transferring and allocating are not convenient enough, and the safety is not high enough. A culture apparatus with a large unit quantity, convenient operation and high operation safety is urgently needed.

In view of this, the present application is specifically made.

Disclosure of Invention

The first purpose of the invention is to provide a poultry cell culture device which has a simple structure, is convenient to use, can provide enough culture space and enough culture units, is not easy to pollute the culture environment in the operation process, is very convenient to take, transfer and allocate, has high safety, and is beneficial to further improving the culture quality and the culture efficiency.

The second purpose of the invention is to provide a poultry cell culture method, which has simple process, convenient execution, difficult pollution to culture environment, convenient taking, transferring and allocating, high safety and contribution to further improving the culture quality and the culture efficiency.

The embodiment of the invention is realized by the following steps:

a poultry cell culture apparatus, comprising: the culture dish comprises a base, a support plate, a first culture dish, a second culture dish, a third culture dish, a transmission gear and a driving rack.

Two backup pads are parallel, the interval sets up and all install in the base. First culture dish, second culture dish and third culture dish are all located between two backup pads, and second culture dish detachably installs in the backup pad, and first culture dish is located one side of keeping away from the base of second culture dish, and the third culture dish is located one side that is close to the base of second culture dish. The first culture dish and the third culture dish are slidably fitted to the support plate. The first culture dish has a first rack and the third culture dish has a second rack.

The transmission gear is rotatably arranged on the supporting plate, and the first rack and the second rack are meshed with the transmission gear. The driving rack is matched with the supporting plate in a sliding mode and meshed with the transmission gear to indirectly drive the first culture dish and the third culture dish to slide out.

Furthermore, the base is provided with a rotating shaft, the driving rack is arc-shaped, and the driving rack is fixedly connected with the rotating shaft through a connecting sheet. The circle center of the arc corresponding to the driving rack is positioned on the central axis of the rotating shaft. The inner side of the driving rack is meshed with the driving gear.

Further, the poultry cell culture device also comprises an outer shell, the base is slidably matched with the inner bottom wall of the outer shell, and the outer shell is provided with an opening for the base to slide out. The rotating shaft is provided with a first extension arm extending along the radial direction of the rotating shaft and a second extension arm extending along the circumferential direction of the rotating shaft, and the second extension arm is connected with the first extension arm. The inner bottom wall of the outer shell is provided with a matching groove matched with the second extension arm.

The base has a first sliding stop and a second sliding stop. When the base is located at the first sliding stop point, the base is accommodated in the outer shell. When the base is positioned at the second sliding dead point, the base extends out of the opening, and the second extension arm is aligned with the matching groove.

The driving rack is provided with a first rotation stop point and a second rotation stop point along the rotating shaft. When the driving rack is positioned at the first rotation stop point, the first culture dish, the second culture dish and the third culture dish are arranged in a stacked mode and are all contained between the supporting plates. When the driving rack is located the second rotation stop point, the first culture dish and the third culture dish slide out from between the supporting plates along the opposite direction, and the second extension arm is matched with the matching groove.

Furthermore, the connecting sheet is fan-shaped, the rotating shaft is also provided with a reference rod which extends along the radial direction of the rotating shaft, the reference rod and the connecting sheet are fixedly connected through a connecting rod which is arc-shaped, and the outer walls of the connecting rod, the connecting sheet and the reference rod are coplanar. When the driving rack is positioned at the first rotation stop point, the reference rod is parallel to the inner bottom wall of the outer shell and arranged along the sliding direction of the base, and the reference rod is attached to the inner bottom wall of the outer shell.

Furthermore, the outer shell is also provided with a screw rod, a movable sleeve and a retaining ring. The bottom wall body of shell body has the inner chamber, and the lead screw is rotationally installed in the inner chamber, and the tip of lead screw runs through to the outside of shell body and installs adjusting hand wheel. The movable sleeve is arranged in the inner cavity and sleeved on the screw rod, and the movable sleeve is in transmission fit with the screw rod. Along the axial direction of the screw rod, the movable sleeve is movably matched with the outer shell. Along the circumference of the screw rod, the movable sleeve is fixedly matched with the outer shell.

The outer wall of the movable sleeve is provided with an adjusting rack arranged along the axial direction of the movable sleeve, the inner bottom wall of the outer shell is provided with a notch communicated with the inner cavity, and the retaining ring is rotatably matched with the notch. The retaining ring is an open arc-shaped ring, the retaining ring is provided with external teeth, and the retaining ring is meshed with the movable sleeve so as to be used for driving the retaining ring to be detachably buckled on the reference rod.

Further, the reference bar has a fitting recess for fitting with the snap ring, the fitting recess being formed by an outer wall thereof recessed in a radial direction thereof. The length of the concave matching part is the same as the axial length of the retaining ring along the length direction of the reference rod.

Further, the backup pad includes first plate body and second plate body, and first plate body and second plate body are parallel, the interval sets up, and the two is fixed connection in the base, and the second plate body is located the inboard of first plate body. The transmission gear is arranged between the first plate body and the second plate body, the first rack and the second rack both penetrate through the second plate body, and the driving rack penetrates through the first plate body.

Further, the tops of the first plate body and the second plate body are fixedly connected through the first connecting block. Still fixedly connected with second connecting block between first plate body and the second plate body, the second fixed block is located the region between first culture dish and the third culture dish.

Further, first culture dish, second culture dish and third culture dish all are on a parallel with the base setting, and the sliding direction of the two of first culture dish and third culture dish all is on a parallel with the base setting.

A method for culturing poultry cells by using the poultry cell culture device comprises the following steps: and rotating the adjusting hand wheel to enable the retaining ring to exit from the matching concave part, and sliding the base to a second sliding stop point. The driving rack is rotated to a second rotation stop point, the first culture dish and the third culture dish slide out from the supporting plate along the opposite direction, and the second extension arm is matched with the matching groove to fix the base. And adding culture medium and primary cells into the first culture dish, the second culture dish and the third culture dish, and adjusting the culture environment to a preset environment. And rotating the driving rack to a first rotation stop point, sliding the base to a first sliding stop point, and rotating the adjusting hand wheel to enable the retaining ring to be buckled into the matching concave part so as to fix the base.

The embodiment of the invention has the beneficial effects that:

in the use process of the poultry cell culture device provided by the embodiment of the invention, the first culture dish, the second culture dish and the third culture dish are limited by the two support plates, and the first culture dish, the second culture dish and the third culture dish are arranged in a stacking mode under normal conditions, so that the three culture dishes can be arranged more compactly, more culture units can be conveniently arranged, and more culture spaces are provided.

On the other hand, when needing to carry out corresponding processing or operation to culture solution or sample, drive the rack through the operation, can make originally first culture dish, second culture dish and the third culture dish in the laminating state stagger each other to provide the space of stepping down that can operate each culture unit, make taking, shifting, allotment to each culture unit very convenient.

In addition, whole operation process need not each culture dish of direct touch, and the backup pad can also play the guard action to each culture dish, greatly reduced in the operation process culture unit receive the possibility of polluting. This makes the overall safety of use higher.

The poultry cell culture device further improves the isolation degree of the sample (culture unit) in the operation process, effectively ensures the safety of the culture unit, improves the smoothness degree and the simplification degree of the operation, and optimizes and improves the culture quality and the culture efficiency of the cells.

Generally, the poultry cell culture device provided by the embodiment of the invention has the advantages of simple structure, convenience in use, capability of providing a large enough culture space and a sufficient number of culture units, difficulty in polluting a culture environment in the operation process, convenience in taking, transferring and allocating, high safety and contribution to further improving the culture quality and the culture efficiency. The poultry cell culture method provided by the embodiment of the invention has the advantages of simple flow, convenient execution, difficult pollution to culture environment, convenient taking, transferring and allocating, high safety and contribution to further improving the culture quality and the culture efficiency.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic structural view of a poultry cell culture apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of the structure of a plate stack of the poultry cell culture apparatus of FIG. 1;

FIG. 3 is a schematic view of the external configuration of the stacked assembly of culture dishes of FIG. 2 at a first rotation stop point;

FIG. 4 is a schematic view of the outer structure of the stacked assembly of culture plates of FIG. 2 at a second stop point of rotation;

FIG. 5 is a schematic diagram of the internal structure of the stacked group of plates of FIG. 3;

FIG. 6 is a schematic diagram of the internal structure of the stacked set of plates of FIG. 4;

FIG. 7 is a schematic diagram of the internal structure of the stacked group of plates of FIG. 3 from another perspective;

FIG. 8 is a schematic view of the assembly of the stacked culture dish assemblies with the outer housing;

FIG. 9 is an enlarged view of area A of FIG. 8;

FIG. 10 is a schematic view of the buckle in a first operating state;

FIG. 11 is a schematic view of the second operating state of the buckle.

Icon: a poultry cell culture apparatus 1000; a base 100; a rotating shaft 110; a connecting piece 120; a first extension arm 130; a second extension arm 140; a reference bar 150; a fitting recess 151; a connecting rod 160; a support plate 200; a first plate 210; a second plate 220; the first connection block 230; a second connection block 240; a first culture dish 300; a first rack 310; a second culture dish 400; a third culture dish 500; a second rack 510; a transmission gear 600; a first ring gear 610; a second ring gear 620; a drive rack 700; an outer housing 800; a mating groove 810; a lead screw 820; a movable sleeve 830; an adjustment rack 840; a retaining ring 850; an inner cavity 860; the hand wheel 870 is adjusted.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

The terms "first," "second," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Examples

Referring to fig. 1 to 7, the present embodiment provides a poultry cell culture apparatus 1000. The poultry cell culture apparatus 1000 includes: a base 100, a support plate 200, a first culture dish 300, a second culture dish 400, a third culture dish 500, a transmission gear 600 and a driving rack 700.

The two support plates 200 are arranged in parallel, and the two support plates 200 are parallel and spaced and are both fixedly mounted on the base 100.

The first culture dish 300, the second culture dish 400 and the third culture dish 500 are all arranged between the two support plates 200, the second culture dish 400 is detachably mounted on the support plates 200, the first culture dish 300 is positioned on one side of the second culture dish 400 far away from the base 100, and the third culture dish 500 is positioned on one side of the second culture dish 400 close to the base 100. The third culture dish 500 has a gap with the base 100. There is also a gap between adjacent three of the first, second and

third culture dishes

300, 400 and 500.

Both sides of the first culture dish 300 and the third culture dish 500 are slidably fitted to the support plate 200. The first culture dish 300 has a first rack 310 and the third culture dish 500 has a second rack 510.

The transmission gear 600 is rotatably installed on the support plate 200, the transmission gear 600 is correspondingly disposed in an area between the first culture dish 300 and the third culture dish 500, the teeth of the first rack 310 and the second rack 510 are oppositely disposed, the first rack 310 and the second rack 510 are disposed in parallel and at intervals, the transmission gear 600 is located between the first rack 310 and the second rack 510, and the first rack 310 and the second rack 510 are both meshed with the transmission gear 600.

The driving rack 700 is slidably fitted to the support plates 200 and engaged with the transmission gear 600 to indirectly drive the first and

third culture dishes

300 and 500 to slide out or drive the first and

third culture dishes

300 and 500 to slide back between the two support plates 200.

In the use, first culture dish 300, second culture dish 400 and third culture dish 500 are spacing by two backup pads 200, and under the general condition, the three is put with range upon range of mode, can place compacter, is convenient for place more culture unit, provides more culture space.

On the other hand, when the culture solution or the sample needs to be correspondingly processed or operated, the driving rack 700 is operated, so that the first culture dish 300, the second culture dish 400 and the third culture dish 500 which are originally in the laminated state are staggered with each other, and an abdicating space capable of operating each culture unit is provided, so that the taking, transferring and allocating of each culture unit are very convenient.

In addition, whole operation process need not each culture dish of direct touch, and the backup pad 200 can also play the guard action to each culture dish, greatly reduced in the operation process culture unit receive the possibility of polluting. This makes the overall safety of use higher.

The poultry cell culture apparatus 1000 further improves the degree of "isolation" of the sample (culture unit) during the operation process, effectively ensures the safety of the culture unit, and improves the smoothness and simplification degree of the operation, so that the culture quality and the culture efficiency of the cells are optimized and improved.

Generally, poultry cell culture apparatus 1000 simple structure, convenient to use can provide enough big cultivation space and enough quantity culture unit to be difficult to cause the pollution to the cultivation environment in operation process, take, shift, allotment are very convenient, and the security is high, is favorable to further promoting to cultivate quality and cultivation efficiency.

Further, referring to fig. 8 to 9, the base 100 is provided with a rotating shaft 110, the driving rack 700 is arc-shaped, and the driving rack 700 and the rotating shaft 110 are fixedly connected by a connecting piece 120.

The plane of the driving rack 700 is perpendicular to the rotating shaft 110, and the center of the arc corresponding to the driving rack 700 is located on the central axis (i.e. the rotating axis) of the rotating shaft 110. The driving rack 700 is toothed at a side thereof adjacent to the rotation shaft 110, and the driving rack 700 is engaged with the driving gear at a side thereof adjacent to the rotation shaft 110 (i.e., an inner side thereof).

The poultry cell culture apparatus 1000 further comprises an outer casing 800, the outer casing 800 is in a square frame shape, the base 100 is slidably fitted to the inner bottom wall of the outer casing 800, and an opening for sliding out the base 100 is formed in the front end wall of the outer casing 800. The plurality of bases 100 are arranged at regular intervals in the width direction of the outer case 800, and the sliding directions of the plurality of bases 100 are arranged in parallel.

The rotation shaft 110 has a first extension arm 130 extending along a radial direction thereof and a second extension arm 140 extending along a circumferential direction thereof, and an end of the second extension arm 140 is connected to an end of the first extension arm 130 away from the rotation shaft 110. The inner bottom wall of the outer case 800 is formed with a fitting groove 810 for fitting with the second extension arm 140.

The base 100 has a first sliding stop and a second sliding stop. When the base 100 is located at the first sliding stop, the base 100 is received in the outer housing 800. When the base 100 is at the second sliding stop, the base 100 slides to the opening and extends outward from the opening, and the second extending arm 140 is aligned with the engaging groove 810.

The driving rack 700 has a first rotation stop point and a second rotation stop point along the rotation shaft 110. When the driving rack 700 is located at the first rotation stop point, the first petri dish 300, the second petri dish 400 and the third petri dish 500 are arranged in a stacked manner and are all accommodated between the supporting plates 200. When the driving rack 700 is located at the second rotation dead point, the first culture dish 300 and the third culture dish 500 slide out from between the supporting plate 200 along the opposite direction, and the second extension arm 140 rotates along with the rotating shaft 110 to be matched with the matching groove 810, so that the base 100 is locked by using the second extension arm 140, the base 100 can be stably matched with the second rotation dead point, the base 100 is prevented from being displaced, and the specific operation of each culture unit is more convenient.

Through the above design, the outer case 800 can protect each of the stacked groups of culture dishes to ensure that the culture process is not affected by the outside. When a specific operation is required for a certain dish stacking group, the corresponding base 100 is slid from the first sliding stop point to the second sliding stop point, and the driving rack 700 is rotated from the first rotating stop point to the second rotating stop point, so that the corresponding first and

third dishes

300 and 500 can be unfolded, and each culture unit can be operated.

After the operation is finished, resetting according to the reverse sequence.

In this embodiment, the connecting piece 120 is fan-shaped, the rotating shaft 110 further has a reference rod 150 extending along the radial direction thereof, the reference rod 150 and the connecting piece 120 are fixedly connected by an arc-shaped connecting rod 160, and the outer walls of the connecting rod 160, the connecting piece 120 and the reference rod 150 are coplanar. The center of the arc corresponding to the connecting rod 160 is also located on the central axis of the rotating shaft 110.

When the driving rack 700 is located at the first rotation dead point, the reference bar 150 is parallel to the inner bottom wall of the outer housing 800 and is disposed along the sliding direction of the base 100, and the reference bar 150 is attached to the inner bottom wall of the outer housing 800.

The connecting pieces 120, the reference bars 150, and the connecting bars 160 are located outside the support plate 200, and are used for adjustment while facilitating the application of an urging force when sliding the base 100.

Further, please refer to fig. 10 to 11, the outer housing 800 further has a screw 820, a movable sleeve 830 and a retaining ring 850.

The bottom wall of the outer shell 800 is provided with an inner cavity 860, the screw 820 is rotatably mounted in the inner cavity 860, and the end of the screw 820 penetrates to the outside of the outer shell 800 and is provided with an adjusting hand wheel 870. The movable sleeve 830 is disposed in the inner cavity 860 and sleeved on the lead screw 820, and the movable sleeve 830 is in transmission fit with the lead screw 820.

The movable sleeve 830 is movably engaged with the outer housing 800 along the axial direction of the screw 820. The movable sleeve 830 is fixedly matched with the outer shell 800 along the circumferential direction of the screw 820.

The outer wall of the movable sleeve 830 is provided with an adjusting rack 840 arranged along the axial direction, the inner bottom wall of the outer shell 800 is provided with a notch communicated with the inner cavity 860, and the retaining ring 850 is rotatably matched with the notch.

The retaining ring 850 is an open arc-shaped ring, the retaining ring 850 has external teeth, and the retaining ring 850 is engaged with the movable sleeve 830 for driving the retaining ring 850 to be detachably fastened to the reference rod 150.

Specifically, the adjusting handwheel 870 is rotated to drive the movable sleeve 830 to move along the axial direction of the screw 820, so that the adjusting rack 840 is used for driving the retaining ring 850 to rotate. When the base 100 is located at the first sliding dead point, the retaining ring 850 can be rotated in the notch by rotating the adjusting hand wheel 870, so that the retaining ring 850 is fastened to the reference rod 150 through the opening thereof (the state is changed from the state shown in fig. 11 to the state shown in fig. 10), thereby fixing the base 100 by fixing the reference rod 150. On one hand, the base 100 is ensured to be stably matched in the outer shell 800, and the sample loss caused by accidental sliding of the base 100 is avoided; on the other hand, the reference rod 150 is locked by the retaining ring 850, and the reference rod 150 is attached to the inner bottom wall of the outer case 800, thereby ensuring that the driving rack 700 is stably positioned at the first rotation stop point, and preventing the first petri dish 300 and the third petri dish 500 from sliding and causing sample loss.

It should be noted that the second extension arm 140 also has the function of preventing the driving rack 700 from rotating accidentally, and when the base 100 is located at the first sliding dead point, if the driving rack 700 rotates, the second extension arm 140 will abut against the inner bottom wall of the outer casing 800, so as to prevent the driving rack 700 from moving. The second extension arm 140 cooperates with the clasp 850 to greatly improve overall state stability and safety.

In the present embodiment, the reference bar 150 has a fitting recess 151 for fitting with the snap ring 850, the fitting recess 151 being formed by an outer wall thereof recessed in a radial direction thereof. The length of the recessed mating portion is the same as the axial length of the snap ring 850 along the length of the reference bar 150. With this design, after the snap ring 850 is fitted to the fitting recess 151, not only can the reference bar 150 be secured to the inner bottom wall of the outer case 800, but also the base 100 can be prevented from sliding.

Further, the supporting plate 200 includes a first plate 210 and a second plate 220, the first plate 210 and the second plate 220 are disposed in parallel and at an interval, and both are fixedly connected to the base 100, and the second plate 220 is located at an inner side of the first plate 210. The first plate 210 and the second plate 220 are both disposed perpendicular to the base 100.

A transmission gear 600 is provided between the first plate 210 and the second plate 220, the transmission gear 600 having a first ring gear 610 and a second ring gear 620.

The first, second, and

third culture dishes

300, 400, and 500 are all disposed parallel to the base 100, and the sliding directions of both the first and

third culture dishes

300 and 500 are disposed parallel to the base 100. Both sides of the first culture dish 300 are provided with first racks 310, and the first racks 310 are disposed along the sliding direction of the first culture dish 300. Both sides of the third culture dish 500 are provided with second racks 510, and the second racks 510 are arranged along the sliding direction of the third culture dish 500.

The second plate 220 is provided with a sliding slot for the first rack 310 and the second rack 510 to slide, and the sliding slot runs through two side panels of the second plate 220, so that the first rack 310 and the second rack 510 can extend from one side of the second plate 220 far away from the first plate 210 to one side of the second plate 220 close to the first plate 210 through the sliding slot. Along the length direction of first rack 310 and second rack 510, the spout has still run through the front and back both ends end wall of second plate body 220, can regard as the spout and divide into three plates with second plate body 220.

The first rack 310 and the second rack 510 are engaged with the second ring gear 620 of the transmission gear 600.

The top portions of the first board 210 and the second board 220 are fixedly connected by a first connection block 230, and the bottom portions of the first board 210 and the second board 220 are connected to the base 100. A second connecting block 240 is fixedly connected between the first plate 210 and the second plate 220, and the second fixing block is located in a region between the first culture dish 300 and the third culture dish 500. That is, the three blocks of the second plate 220 are fixed by the first connection block 230, the second connection block 240, and the base 100, respectively.

The first plate body 210 is provided with a circular arc-shaped rack slot for the driving rack 700 to slide, and the rack slot penetrates through the first plate body 210, so that the driving rack 700 can extend to one side of the first plate body 210 close to the second plate body 220 and be engaged with the first gear ring 610 of the transmission gear 600.

Through the design, flexible transmission and control among all parts are realized. In addition, the first plate 210 and the second plate 220 can protect the transmission components, thereby ensuring smooth transmission.

This embodiment also provides a method for culturing poultry cells using the poultry cell culture apparatus 1000 described above, including: the adjustment hand wheel 870 is rotated to withdraw the retaining ring 850 from the fitting recess 151, and the base 100 is slid to the second sliding stop point. The driving rack 700 is rotated to the second rotation dead point, the first and

third culture dishes

300 and 500 are slid out in opposite directions from between the support plates 200, and the second extension arms 140 are fitted to the fitting grooves 810 to fix the base 100. The first, second and

third culture dishes

300, 400 and 500 are filled with culture medium and primary cells, and the culture environment is adjusted to a predetermined environment. The driving rack 700 is rotated to the first rotation stop point, the base 100 is slid to the first sliding stop point, and the adjusting hand wheel 870 is rotated to make the retaining ring 850 snap into the fitting recess 151, thereby fixing the base 100.

Details of other operations have been described above, and are not described herein.

To sum up, poultry cell culture apparatus 1000 simple structure, convenient to use can provide enough big cultivation space and enough quantity culture unit to be difficult to cause the pollution to the cultivation environment in operation process, take, shift, allotment are very convenient, and the security is high, is favorable to further promoting to cultivate quality and cultivation efficiency. The poultry cell culture method has simple flow, convenient execution, difficult pollution to the culture environment, convenient taking, transferring and allocating, high safety and contribution to further improving the culture quality and the culture efficiency.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A poultry cell culture apparatus, comprising: the culture device comprises a base, a support plate, a first culture dish, a second culture dish, a third culture dish, a transmission gear and a driving rack;

the two supporting plates are arranged in parallel and at intervals and are both arranged on the base; the first culture dish, the second culture dish and the third culture dish are arranged between the two support plates, the second culture dish is detachably arranged on the support plates, the first culture dish is positioned on one side, far away from the base, of the second culture dish, and the third culture dish is positioned on one side, close to the base, of the second culture dish; the first culture dish and the third culture dish are both slidably fitted to the support plate; the first culture dish is provided with a first rack, and the third culture dish is provided with a second rack;

the transmission gear is rotatably arranged on the supporting plate, and the first rack and the second rack are meshed with the transmission gear; the driving rack is matched with the supporting plate in a sliding mode and meshed with the transmission gear to indirectly drive the first culture dish and the third culture dish to slide out.

2. The poultry cell culture device according to claim 1, wherein the base is provided with a rotating shaft, the driving rack is arc-shaped, and the driving rack and the rotating shaft are fixedly connected through a connecting sheet; the circle center of the arc corresponding to the driving rack is positioned on the central axis of the rotating shaft; the inner side of the driving rack is meshed with the driving gear.

3. The poultry cell culture apparatus of claim 2, further comprising an outer housing, wherein the base is slidably engaged with an inner bottom wall of the outer housing, and wherein the outer housing defines an opening through which the base slides out; the rotating shaft is provided with a first extension arm extending along the radial direction of the rotating shaft and a second extension arm extending along the circumferential direction of the rotating shaft, and the second extension arm is connected with the first extension arm; the inner bottom wall of the outer shell is provided with a matching groove used for matching with the second extension arm;

the base is provided with a first sliding stop point and a second sliding stop point; when the base is positioned at the first sliding stop point, the base is accommodated in the outer shell; when the base is positioned at the second sliding dead point, the base extends out of the opening, and the second extension arm is aligned with the matching groove;

the driving rack is provided with a first rotation stop point and a second rotation stop point along the rotating shaft; when the driving rack is positioned at the first rotation stop point, the first culture dish, the second culture dish and the third culture dish are arranged in a stacked mode and are all contained between the supporting plates; when the driving rack is located at the second rotation stop point, the first culture dish and the third culture dish slide out from the supporting plates along opposite directions, and the second extension arm is matched with the matching groove.

4. The poultry cell culture device according to claim 3, wherein the connecting piece is fan-shaped, the rotating shaft further comprises a reference rod extending along the radial direction of the rotating shaft, the reference rod and the connecting piece are fixedly connected by a connecting rod in an arc shape, and the outer walls of the connecting rod, the connecting piece and the reference rod are coplanar; when the driving rack is located at the first rotation stop point, the reference rod is parallel to the inner bottom wall of the outer shell and is arranged along the sliding direction of the base, and the reference rod is attached to the inner bottom wall of the outer shell.

5. The poultry cell culture apparatus of claim 4, wherein the outer housing further has a screw, a movable sleeve and a retaining ring; the bottom wall body of the outer shell is provided with an inner cavity, the screw rod is rotatably arranged in the inner cavity, and the end part of the screw rod penetrates through the outer part of the outer shell and is provided with an adjusting hand wheel; the movable sleeve is arranged in the inner cavity and sleeved on the screw rod, and the movable sleeve is in transmission fit with the screw rod; the movable sleeve is movably matched with the outer shell along the axial direction of the screw rod; the movable sleeve is fixedly matched with the outer shell along the circumferential direction of the screw rod;

the outer wall of the movable sleeve is provided with an adjusting rack arranged along the axial direction of the movable sleeve, the inner bottom wall of the outer shell is provided with a notch communicated with the inner cavity, and the retaining ring is rotatably matched with the notch; the retaining ring is an open arc-shaped ring, the retaining ring is provided with external teeth, and the retaining ring is meshed with the movable sleeve so as to be used for driving the retaining ring to be detachably buckled on the reference rod.

6. The poultry cell culture apparatus according to claim 5, wherein the reference bar has a fitting recess for fitting with the retaining ring, the fitting recess being formed by an outer wall thereof being recessed in a radial direction thereof; the length of the concave matching part is the same as the axial length of the retaining ring along the length direction of the reference rod.

7. The poultry cell culture apparatus of claim 6, wherein the support plate comprises a first plate and a second plate, the first plate and the second plate are parallel and spaced apart and both are fixedly connected to the base, and the second plate is located inside the first plate; the transmission gear is arranged between the first plate body and the second plate body, the first rack and the second rack penetrate through the second plate body, and the driving rack penetrates through the first plate body.

8. The poultry cell culture apparatus of claim 7, wherein the top portions of the first plate and the second plate are fixedly connected by a first connecting block; still fixedly connected with second connecting block between the first plate body with the second plate body, the second fixed block is located first culture dish with the region between the third culture dish.

9. The poultry cell culture apparatus of claim 8, wherein the first, second and third culture dishes are all disposed parallel to the base, and the sliding direction of both the first and third culture dishes are disposed parallel to the base.

10. A poultry cell culture method using the poultry cell culture apparatus according to claim 9, comprising:

rotating the adjusting hand wheel to enable the retaining ring to exit from the matching concave part and slide the base to a second sliding stop point;

rotating the driving rack to the second rotation stop point, sliding the first culture dish and the third culture dish out from between the support plates in opposite directions, and matching the second extension arm to the matching groove to fix the base;

adding culture medium and primary cells into the first culture dish, the second culture dish and the third culture dish, and adjusting the culture environment to a preset environment;

and rotating the driving rack to the first rotation dead point, sliding the base to the first sliding dead point, and rotating the adjusting hand wheel to enable the retaining ring to be buckled into the matching concave part so as to fix the base.