CN111187712A

CN111187712A - Aseptic liquid transfer device and application thereof

Info

Publication number: CN111187712A
Application number: CN202010050062.9A
Authority: CN
Inventors: 王军峰
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-01-16
Filing date: 2020-01-16
Publication date: 2020-05-22

Abstract

The invention discloses an aseptic liquid transfer device and application thereof, wherein the aseptic liquid transfer device comprises a transfer bottle and a transfer port; the transfer bottle is provided with two openings and a conduit; the adapter is of a flange structure, the tubular axis of the adapter is composed of two sections, the inner diameter of the inner tube of the first section is smaller and is close to the outer diameter of the catheter, and the inner diameter of the inner tube of the second section is larger; the outer port of the guide pipe faces the side wall direction of the guide pipe, two annular sealing grooves are formed in the position close to the outer port, the guide pipe on the outer side of the switching bottle penetrates through the first section of inner pipe of the switching port, a sealing gland is fixed at the tail end of the guide pipe, when the guide pipe is pulled to one side of the switching bottle, the sealing gland is in edge contact with the first section of inner pipe of the switching port, the outer port of the guide pipe is sealed, and when the switching bottle is pushed to one side of the switching port, the outer port of the guide pipe extends out of the first section of inner pipe. The aseptic liquid-transfering device can realize on-line sterilization, is suitable for aseptic inoculation in biological process, and can also be used for aseptic sampling in biological process.

Description

Aseptic liquid transfer device and application thereof

Technical Field

The invention relates to a sterile liquid transfer device and application thereof, in particular to a disposable sterile liquid transfer device and application thereof, and belongs to the technical field of biology.

Background

At present, the biological culture process belongs to pure culture, and the inoculation and sampling process needs strict sterile environment, so that on one hand, a sample is prevented from being polluted, and on the other hand, the culture environment in a bioreactor is prevented from being polluted, so that a sampling and inoculation device capable of sterilizing on line is particularly needed. However, at present, the sampling device and the inoculation device are complex in structure, inconvenient to carry and complex to operate.

Disclosure of Invention

The invention aims to solve the technical problems of more valve pipelines, complex structure, difficult cleaning after use and the like of the sampling or inoculating device, and provides the device which can be used for inoculating and sterile sampling and can be sterilized on line, and a plurality of valves are saved by integrally designing the transfer bottle and the transfer port of the sterile liquid transfer device, so the device is light and portable; and through processing switching bottle and switching mouth into disposable consumptive material, disinfect in advance, connect this device to a tee bend through the clamp during use and carry out online sterilization, then just simply push away this device with the hand, alright realize the function to a plurality of valve operations before, consequently to sample and inoculation, the operation is very simple and direct.

The technical scheme of the invention is as follows:

an aseptic liquid transfer device comprises a transfer port of a transfer bottle, wherein the transfer bottle is provided with two openings and a conduit, one opening is a breathing port, an air filter is arranged on the breathing port, the other opening is a liquid transfer port and is sealed by a cover, the conduit is provided with two ports, an inner port is arranged inside the transfer bottle and is close to the bottom of the transfer bottle, an outer port is arranged outside the transfer bottle, and the opening faces the side wall direction of the transfer bottle; 2 annular sealing grooves are formed in the part, outside the adapter bottle, of the guide pipe, sealing rings are placed in the sealing grooves, the first sealing groove is a sealing groove close to the adapter bottle, and the second sealing groove is a sealing groove far away from the adapter bottle; the volume of the transfer bottle is 5mL-5000 mL.

The adapter is a flange structure, and its tubulose axle center comprises two sections, and first section inner tube internal diameter is less, slightly is greater than the external diameter of pipe, and second section inner tube internal diameter is great, both sides around the adapter divides, and the one side that the internal diameter is less is the front side, and the one side that the internal diameter is great is the rear side.

The outer end port of the conduit is positioned between the second sealing groove and the sealing gland, and the shortest distance from the center of the outer end port to the side wall of the transfer bottle positioned on the front side of the transfer port is greater than the axial length of the first section of inner tube of the transfer port.

The adapter bottle is characterized in that a section of spring is sleeved on an external guide pipe of the adapter bottle, the tail end of the guide pipe penetrates through the front side of an adapter port, a sealing gland is fixed at the tail end of the guide pipe, and the outer diameter of the sealing gland is between the inner diameter of a first section of inner pipe and the inner diameter of a second section of inner pipe of the adapter port; the adapter and the adapter bottle are combined into a whole by the method.

The plane of the annular sealing groove on the guide pipe is vertical to the axial direction of the guide pipe, and the distance between the planes of the two sealing grooves is 5mm-200 mm.

After the adapter bottle and the adapter port are combined through the catheter, the spring in the middle is in a compressed state, the spring force can enable the adapter bottle and the adapter port to be separated towards two sides until the sealing gland at the tail end of the catheter is in contact with the edge of the first section of the inner pipe in the adapter port, and the initial state of the device is shown in figure 4.

When the sterile liquid transfer device is in an initial state, the shortest distance between the first sealing groove and the side wall of the adapter bottle positioned on the front side of the adapter port is required to be not less than the shortest distance between the side wall of the adapter bottle positioned on the position and the side surface of the front side of the adapter port and not more than the shortest distance between the first sealing groove and the second sealing groove, and the shortest distance between the first sealing groove and the second sealing groove is required to be less than the axial length of the first section of inner tube of the adapter port.

The settings can ensure that the first sealing groove cannot move out of the adapter when the tail end of the catheter moves towards one side of the adapter bottle, and the first sealing groove cannot reach the position of the second sealing groove in the initial state when the catheter moves away from one side of the adapter bottle, so that the outer port of the catheter cannot be polluted; inside the switching mouth, dispose a packing ring at the second section inner tube, can further promote this device's leakproofness.

The materials of the transfer bottle and the transfer port are glass, metal, silica gel, resin, waterproof paperboards, plastics or rubber and the like.

The aseptic liquid transfer device is filled into an aseptic bag after being sterilized, or is filled into a packaging bag and then sterilized, is independently stored, and is immediately used after being unpacked; when sampling or inoculating, fix the switching mouth on the flange on the sampling pipeline or inoculation pipeline, when pushing the switching bottle to switching mouth one side with the hand, the outer port of pipe stretches out from switching mouth first section inner tube to make the switching bottle communicate with the exterior space (figure 1).

The sterile liquid transfer device can be used for inoculation or process sampling of a fermentation tank, inoculation or sampling in a cell culture process, sampling of water for injection, sampling of liquid food, sampling of cosmetics, sampling of blood products, sampling of clean gas and the like.

The use method of the sterile liquid transfer device for the sampling process is specifically shown in fig. 5:

firstly, taking a tee joint, connecting a first tee joint in the tee joint with a sampling pipe of a device to be sampled, connecting a second tee joint in the tee joint with a discharge valve 8, and then connecting a third tee joint in the tee joint with a switching port flange plate through a clamp;

opening a steam valve 6 on a sampling pipe of the device to be sampled, slowly opening a discharge valve 8 at the lower end of the tee joint to allow a small amount of steam to be emitted so as to keep a certain steam pressure in the sampling pipe, allowing the steam to enter the tee joint from top to bottom along the pipe, performing high-temperature sterilization on the outer side surface at the rear side of the adapter port, closing the steam valve 6 after 10 minutes, opening a liquid discharge valve 7 of the device to be sampled, and closing the discharge valve 8 after liquid flows out;

then, the transfer bottle is pushed towards the direction of the transfer port by hand, when the transfer bottle is contacted with the transfer port, the pipe port already extends out of the first section of the inner pipe of the transfer port, so that the transfer bottle is communicated with the sampling pipeline, the culture solution can enter the transfer bottle, when the volume of the culture solution meets the requirement, the external force for pushing the transfer bottle is removed, at the moment, the transfer bottle returns to the initial position under the action of the spring, and the transfer bottle is sealed;

then, closing a liquid discharging valve 7 of the device to be sampled, opening a discharge valve 8, opening a steam valve 6, removing the culture solution in the pipeline by using steam, and closing the steam valve 6;

and after sampling is finished, opening the clamps of the tee joint and the adapter, and taking down the sterile liquid transfer device. Then, in the sterile room, the liquid transfer port of the transfer bottle is opened, and the culture solution is taken out from the corresponding outlet-use instrument.

The use method of the sterile liquid transfer device for the inoculation process comprises the following steps:

firstly, taking out the sterile liquid transfer device in a sterile room, opening a liquid transfer port, transferring the seed liquid to be inoculated into a transfer bottle, then sealing the liquid transfer port by using a cover, and then bringing the sterile liquid transfer device to the side of a fermentation tank to be inoculated; when the transfer bottle is in contact with the transfer port, the transfer bottle is communicated with the sampling pipeline, and at the moment, the seed liquid can enter the reactor under the action of pressure to realize the function of inoculation.

The invention has the beneficial technical effects that:

according to the aseptic liquid transfer device, the transfer bottle and the transfer port of the aseptic liquid transfer device are integrally designed, so that a plurality of valves are omitted, and the aseptic liquid transfer device is simple in structure and convenient to carry;

in addition, the aseptic liquid transfer device provided by the invention has the advantages that the disposable consumable material is processed by combining the transfer bottle and the transfer port, the sterile consumable material is bagged after being sterilized in advance, and the sterile consumable material is taken out and directly connected to the tee joint when being used, so that one-time sterilization operation is reduced, cleaning is avoided, the operation time is saved, and the operation cost is reduced;

furthermore, the sterile liquid transfer device can be used for sampling and inoculating, so that the application range is enlarged.

Therefore, the invention has the advantages of simple structure, convenient operation, obvious reduction of sampling cost, wide application and the like.

Drawings

FIG. 1 is a schematic view of an adapter bottle of the sterile liquid transfer device in a communicating state;

FIG. 2 is a cross-sectional view of the adapter;

FIG. 3 is a schematic diagram showing the dimensions of the catheter outside the adapter bottle;

FIG. 4 is a schematic view of the sterile liquid transfer apparatus in an initial state;

fig. 5 is a schematic view of the general structure of the sterile liquid transfer device of example 2.

Detailed description of the preferred embodiments

The invention is further illustrated, but not limited, by the following specific examples in connection with the accompanying drawings.

Example 1

The structure and the size of a specific sterile liquid transfer device are shown in fig. 1, and the sterile liquid transfer device comprises a transfer bottle 1 and a transfer port 2, wherein the volume of the transfer bottle is 200 mL;

as shown in fig. 1, the transfer bottle 1 is provided with two openings at the upper part of the bottle wall of the transfer bottle 1, one opening is a breathing port 13, the breathing port 13 is used for installing an air filter 4, the other opening is a liquid transfer port 12, and the transfer bottle 1 is sealed by a cover 5, and is also provided with a conduit 11, the conduit is provided with two outlets, wherein the first outlet is positioned in the transfer bottle, the outlet is close to the bottom of the transfer bottle, the outer port 14 of the conduit faces the side of the conduit, the tail end of the conduit is provided with a sealing gland 15, one section of the conduit close to the outer port is provided with 2 sealing grooves 16, and sealing rings with corresponding sizes are placed in the sealing grooves;

the adapter port 2 is arranged on the right side of the adapter bottle 1;

the adapter 2 is shown in fig. 2 specifically, and includes an adapter rear side 21, a flange 22, a gasket 23, a second section of inner tube 24, and a first section of inner tube 25; the section of the adapter is in a T shape, the outer diameter of the front side is 18mm, the axial length is 20mm, and the outer diameter of the rear side is 50 mm;

1 spring 3 is arranged between the adapter bottle and the adapter port, the natural length of the spring is 42mm, one side of the spring contacts with the side wall of the adapter bottle, and the other side of the spring passes through the smaller outer diameter of the adapter port and contacts with the flange plate;

the inner diameter of the conduit 11 is 6mm, the outer diameter of the conduit is 10mm, the inner diameter of the inner tube of the first section of the adapter is 11mm, the length of the inner tube of the first section of the adapter is 30mm, the inner diameter of the inner tube of the second section of the adapter is 18mm, and the length of the inner tube of the second section of the adapter is 8 mm;

the outer diameter of the sealing gland 15 of the conduit 11 (refer to fig. 1 and 3) is 16mm, the thickness is 5mm, the length of the outer conduit of the adapter bottle is 45mm (excluding the thickness of the sealing gland), wherein the shortest distance between the first sealing groove and the side wall of the adapter bottle positioned at the front side of the adapter port is 15mm (indicated by a distance a in fig. 3), the distance between the center of the first sealing groove and the center of the second sealing groove is 18mm (indicated by a distance b in fig. 3), the distance between the second sealing groove and the center of the outer port of the conduit is 6mm (indicated by a distance c in fig. 3), and the distance between the center of the outer port of the conduit and the outer side of the sealing gland of the conduit is 11mm (indicated by a distance d in; the inner diameter of the outer port is 6 mm;

the material of the adapter bottle 1 and the adapter port 2 is polypropylene.

Example 2

An aseptic liquid transfer device can be used in the inoculation stage of a fermentation process (see figure 5):

firstly, taking out the sterile liquid transfer device in a sterile room, opening a liquid transfer port, transferring the seed liquid to be inoculated into a transfer bottle, then sealing the liquid transfer port by using a cover, and then bringing the sterile liquid transfer device to the side of a fermentation tank to be inoculated;

taking a tee joint, and connecting a first tee joint in the tee joint with a inoculation pipe of the fermentation tank; then connecting the second of the three-way valve with a discharge valve 8; then, a third tee in the tee is connected with the adapter flange plate through a clamp;

opening a steam valve 6 on a inoculation pipe of the fermentation tank to be inoculated, slowly opening a discharge valve 8 at the lower end of the tee joint to allow a small amount of steam to flow out so as to keep a certain steam pressure in the inoculation pipe, allowing the steam to enter the tee joint from top to bottom along the pipe and diffuse to a transfer port of the sterile liquid transfer device, sterilizing the outer side surface of the rear side of the transfer port at high temperature by the steam, closing the discharge valve 8 after 20 minutes, closing the steam valve 6, opening an inoculation valve 7 of the fermentation tank to be inoculated, and connecting compressed air to an air filter of a transfer bottle;

then, the switching bottle is pushed to the direction of the switching port by hand until the outer wall of the switching bottle is contacted with the outer side surface of the front side of the switching port, the pipe port extends out of the switching port, so that the switching bottle is communicated with an inoculation pipeline, at the moment, seed liquid is transferred into a reactor from the switching bottle under the action of pressure, when the inoculation volume meets the requirement, the switching bottle is not pushed any more, at the moment, the switching bottle moves to the opposite direction under the action of a spring until the switching bottle returns to the initial position, at the moment, the outer port of the pipe retracts into the switching port, a sealing gland is contacted with the edge of the first section of inner pipe of the switching port, the switching bottle is sealed, then, the inoculation valve 7 is closed, the discharge valve 8 is opened, the steam valve 6 is opened, at the moment, the liquid in the pipeline;

and after inoculation is completed, opening the clamp connected with the tee joint and the adapter flange plate, and taking down the sterile liquid transfer device. The rest is the same as in example 1.

Compared with the currently used device, the whole process saves about 1 hour of high-pressure steam sterilization time, and 0.2 hour of device cleaning time; except that save time, the operation of this device is also simpler, only need with the hand push change bottle just can realize the function of preceding to a plurality of valve operations, convenient to carry when business turn over aseptic chamber moreover.

In summary, the present invention provides a liquid transfer device capable of performing online sterilization, and more particularly, to a disposable sterile liquid transfer device, which has the advantages of simple structure, easy operation, significantly reduced sampling cost, and wide application compared with the existing similar devices, and is very suitable for online sterile sampling and inoculation operations in microbial fermentation and cell culture processes.

The foregoing is merely an example of the embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.

Claims

1. An aseptic liquid transfer device is characterized by comprising a transfer bottle and a transfer port;

the adapter bottle is provided with 2 openings and a conduit, one opening is a breathing port, an air filter is arranged on the breathing port, the other opening is a liquid transfer port and is sealed by a cover, the conduit is provided with two ports, the inner port is arranged in the adapter bottle, the opening is close to the bottom of the adapter bottle, the outer port is arranged outside the adapter bottle, and the opening faces the side wall direction of the conduit; 2 annular sealing grooves are formed in the part, outside the adapter bottle, of the guide pipe, sealing rings are placed in the sealing grooves, the first sealing groove is a sealing groove close to the adapter bottle, and the second sealing groove is a sealing groove far away from the adapter bottle;

the adapter is of a flange structure, the tubular axle center of the adapter is composed of two sections, the inner diameter of the inner tube of the first section is smaller and is close to the outer diameter of the guide tube, the inner diameter of the inner tube of the second section is larger, the adapter is divided into a front side and a rear side, the side with the smaller inner diameter is the front side, and the side with the larger inner diameter is the rear side;

the adapter is sleeved on the guide pipe through a tubular shaft center of the adapter, the front side of the adapter faces the adapter bottle, a sealing gland is fixed at the tail end of the guide pipe, and the outer diameter of the sealing gland is between the inner diameter of the first section of inner pipe and the inner diameter of the second section of inner pipe of the adapter.

2. An aseptic liquid transfer device as defined in claim 1, wherein the plane of the annular sealing groove on said tube is perpendicular to the length of the tube, and the distance between the planes of the two sealing grooves is 5mm-150 mm.

3. An aseptic liquid transfer device as defined in claim 1, wherein said adaptor is sleeved on the guide tube through its tubular axis, and when the sealing gland at the end of the guide tube is in contact with the edge of the first section of inner tube in the adaptor, the shortest distance between the first sealing groove and the side wall of the adaptor bottle near the front side of the adaptor is not less than the shortest distance between the side wall of the adaptor bottle at the position and the side surface of the front side of the adaptor, and is not more than the shortest distance between the first sealing groove and the second sealing groove.

4. An aseptic liquid transfer device as defined in claim 1, wherein the shortest distance between said first and second sealing grooves is less than the axial length of the inner tube of the first section of the adaptor port.

5. An aseptic liquid transfer device as defined in claim 1, wherein said outer end of said conduit is positioned between said second sealing groove and said sealing gland, and the shortest distance from the center of said outer end to the side wall of the transfer bottle in front of said transfer port is greater than the axial length of the inner tube in the first section of said transfer port.

6. An aseptic liquid transfer device as defined in claim 1, wherein the tube between said adapter vial and said adapter port has a spring which is compressed when the gland contacts the rim of the inner tube in the first section of the adapter port.

7. An aseptic liquid transfer device as defined in claim 1, wherein said transfer bottle has a volume of 5mL to 5000 mL.

8. An aseptic liquid transfer device as defined in claim 1, wherein said adaptor bottle and adaptor port are made of glass, metal, silicone, resin, waterproof cardboard, plastic or rubber.

9. A sterile liquid transfer device as defined in claim 1 for inoculation or sampling of fermentation broth in a biological fermentation process, inoculation or sampling in a cell culture process, sampling of water for injection, sampling of liquid food products, sampling of cosmetics, sampling of blood products or sterile sampling of clean gas.