CN216141554U - Cell culture bottle - Google Patents

Abstract

The utility model provides a cell culture bottle, which comprises a bottle cap (100) and a bottle body (200), the bottle cap (100) comprises a cap body (110) and a cap (120), the bottle body (200) comprises a bottle mouth (210) and a bottle body (220) which are integrated into a whole, wherein the cap body (110) is connected with the bottle mouth (210) and the cap (120) at two ends respectively, and the cap (120) is provided with an inner tube (122) on an inner surface thereof, an outer tube (125) on an outer surface thereof, and a hose (126) connected to the outer tube (125), wherein the lumens of the inner tube (122) and the outer tube (125) both extend through the cap (120), the flexible tube (126) having a closed free end, and wherein an annular channel (LC) is formed around the inner tube (122), the annular channel (LC) communicates with the lumen of the outer tube (125).

Description

Cell culture bottle

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture bottle suitable for culturing peripheral blood mononuclear cells.

Background

Peripheral blood is blood other than bone marrow, and mononuclear cells in peripheral blood refer to cells having a single nucleus in peripheral blood, and the mononuclear cells include two types of cells, one type is lymphocytes and the other type is monocytes. Lymphocytes are further divided into two types, one being B lymphocytes and the other being T lymphocytes. B lymphocyte has humoral immunity function, under the stimulation of antigen, B lymphocyte morphological deformation can be converted into plasma cell, and the plasma cell can produce secretory antibody, thereby exerting humoral immunity function; t lymphocytes have strong cellular immune function, and can directly kill pathogenic microorganisms or aged cells entering human bodies. Monocytes are one type of human leukocytes, which escape from the blood to tissues and become phagocytic cells, which can phagocytose senescent cells and cell debris, as well as various pathogenic microorganisms that enter the body. Moreover, the mononuclear cells cultured by peripheral blood separation have the characteristics of high proliferation speed, safety, durability, adaptability, systematicness and the like, compared with the traditional methods such as radiotherapy and the like, the mononuclear cells can stimulate the systemic anti-tumor effect through active immunity, have wider action range and are particularly suitable for multiple focuses or malignant tumors with wide metastasis. Therefore, it is a trend in the medical field to culture mononuclear cells isolated from peripheral blood and to treat diseases using the cultured mononuclear cells. However, in the existing culture bottles for culturing the mononuclear cells, the bottle cap is required to be opened repeatedly or a tube is required to be inserted into the bottle repeatedly when the mononuclear cells are inoculated, the culture medium is supplemented, and the mononuclear cells are harvested, so that the operations of inoculation, solution supplementation and harvesting processes are complicated, and the risk of contamination of the mononuclear cells by external bacterial viruses is increased.

Therefore, there is a need in the art for a simple and convenient method for reducing the risk of contamination of mononuclear cells with external bacterial viruses.

SUMMERY OF THE UTILITY MODEL

In order to solve the above-mentioned problems in the prior art, the present invention provides a cell culture bottle comprising a bottle cap and a bottle body, wherein the bottle cap comprises a cap body and a cap, the bottle body comprises a mouth and a body which are integrated, wherein,

the cap body is connected at both ends to the mouth and the cap, respectively, and

the cap is provided with an inner tube on an inner surface thereof, an outer tube on an outer surface thereof, and a flexible tube connected to the outer tube, wherein lumens of the inner and outer tubes both extend through the cap, the flexible tube having a closed free end, and wherein an annular channel is formed around the inner tube, the annular channel communicating with the lumen of the outer tube.

According to an alternative embodiment of the utility model, the annular channel communicates with the lumen of the outer tube at the inner surface of the cap.

According to an alternative embodiment of the utility model, the inner tube and the annular channel extend through the mouth into the body.

According to an alternative embodiment of the present invention, the bottle cap is integrated with the bottle body.

According to an alternative embodiment of the utility model, the body has a first and a second flat side wall opposite to each other, wherein the mouth extends away from the plane of the first side wall.

According to an alternative embodiment of the utility model, in a cross-sectional view perpendicular to the first and second side walls, the side of the mouth close to the second side wall extends from the second side wall in a direction away from the plane of the first side wall.

According to an alternative embodiment of the utility model, the hose is inserted into the outer tube and the joint region of the hose and the outer tube is sealed by means of a potting compound.

According to an alternative embodiment of the utility model, the outer tube is inserted into the hose and the outside of the hose is provided with an elastic tie.

According to an alternative embodiment of the utility model, the outer tube is provided with an annular ridge on its outer surface, which annular ridge is inserted into the hose.

According to an alternative embodiment of the utility model, the elastic band is aligned radially with the annular ridge.

The utility model may be embodied in the form of exemplary embodiments shown in the drawings. It is to be noted, however, that the drawings are designed solely for purposes of illustration and that any variations which come within the teachings of the utility model are intended to be included within the scope of the utility model.

Drawings

The drawings illustrate exemplary embodiments of the utility model. These drawings should not be construed as necessarily limiting the scope of the utility model, wherein:

FIG. 1 is a perspective view of a cell culture flask according to an embodiment of the present invention;

FIG. 2 is an exploded view of the cell culture flask shown in FIG. 1;

FIG. 3 is a partial cross-sectional view of the cell culture flask taken along line III-III in FIG. 1;

FIG. 4 is a partial cross-sectional view of the cell culture flask taken along line IV-IV in FIG. 1; and

FIG. 5 is an exploded view of a cell culture flask according to another embodiment of the present invention.

Detailed Description

Further features and advantages of the present invention will become apparent from the following description, which proceeds with reference to the accompanying drawings. Exemplary embodiments of the utility model are illustrated in the drawings and the various drawings are not necessarily drawn to scale. This invention may, however, be embodied in many different forms and should not be construed as necessarily limited to the exemplary embodiments set forth herein. Rather, these exemplary embodiments are provided only to illustrate the present invention and to convey the spirit and substance of the utility model to those skilled in the art.

The present invention is directed to a cell culture flask suitable for culturing mononuclear cells (e.g., lymphocytes, monocytes) separated from peripheral blood, the cell culture flask generally comprising a flask cap and a flask body, wherein the bottle cap can be detachably or inseparably fixed to the bottle body, and after being fixed to the bottle body, the culture medium required for culturing the mononuclear cells can be conveyed into the bottle body through the pipeline on the bottle cap (also referred to as a fluid replacement operation in the text), and the cultured mononuclear cells are extracted from the bottle body through a pipeline on the bottle cap (also referred to as a harvesting operation in the text), and the liquid supplementing operation and the harvesting operation do not need to take down the bottle cap from the bottle body, thereby simplifying the operation process, but also avoids contamination of the cultured mononuclear cells during removal and reinstallation of the cap. In addition, when the operations of fluid replacement and harvesting are not performed, that is, during the culture of the mononuclear cells, the free end of the tubing is closed, which reduces the risk of contamination of the mononuclear cells by contaminants entering the vial through the tubing. In addition, in order to prevent pressure from being accumulated in the bottle body during the fluid infusion operation and thus prevent the culture medium from entering the bottle body, and in order to prevent a vacuum from being formed in the bottle body during the harvest operation and thus prevent the cultured mononuclear cells from leaving the bottle body, the inside of the bottle body can be also ventilated with the outside through the air holes in the bottle cap so as to balance the air pressure in the bottle body and the outside air pressure, thereby enabling the fluid infusion operation and the harvest operation to be smoothly performed. Further, the bottle cap is further provided with an inner tube extending from the gas hole toward the bottle body, the inner tube being capable of separating liquid (e.g., culture medium and mononuclear cells) entering and exiting the bottle cap from gas, thereby preventing the liquid from leaking to the outside of the bottle body through the gas hole. In conclusion, with the cell culture flask according to the present invention, the operations of culturing, replenishing, and harvesting during the production of mononuclear cells can be performed easily and smoothly, and the risk of contamination of the mononuclear cells can be reduced, thereby producing high-quality mononuclear cells in a simple and reliable manner.

Various embodiments of the cell culture flask according to the present invention are described in detail below with reference to the respective drawings. In the following description, "axial" will be used in its ordinary meaning in the art to refer to the direction of the axis of the ring-shaped structure, while "radial" will be used in its ordinary meaning in the art to refer to the direction of the diameter or radius of a circle centered on the axis, whereas "proximal" will be used herein to refer to the side or end axially closer to the body, and "distal" will be used to refer to the side or end axially further from the body. It is to be understood, however, that these terms, which are indicative of relative orientations, are merely intended to more intuitively convey the concept and teaching of the present invention in connection with the drawings, and should not be construed as limiting the scope of the present invention in any way.

Referring to fig. 1-4, there are shown perspective, exploded and cross-sectional views of a cell culture bottle generally comprising a bottle cap 100 and a bottle body 200, the bottle cap 100 being detachably connectable (e.g., via threads, snap-fit structures, etc.) to the bottle body 200, the bottle body 200 being adapted to store mononuclear cells and culture medium thereof, according to one embodiment of the present invention. In this case, the bottle cap 100 may be coupled to the bottle body 200 after separating mononuclear cells from peripheral blood and inoculating the mononuclear cells in the bottle body 200, so as to prevent contaminants from entering the bottle body through the mouth of the bottle body 200 and to prevent the culture medium and the mononuclear cells from leaking to the outside of the bottle body 200 through the mouth. Alternatively, the bottle cap 100 may be inseparably attached to the bottle body 200 (e.g., by welding, bonding, etc.) or integrally manufactured with the bottle body 200 to be integral therewith. In this case, the liquid may be transferred into and out of the bottle body 200 through a pipe on the bottle cap 100 as described below.

Referring to fig. 2-4, which illustrate exploded and cross-sectional views of a cell culture flask according to the present invention, a cap 100 of the cell culture flask is shown to mainly include a cap body 110 and a cap 120.

The cap body 110 is described in more detail below in conjunction with the drawings, and as shown in fig. 2-4, the cap body 110 has a generally annular structure including a proximal end 111 and a distal end 112 spaced apart in an axial direction, and an intermediate portion 113 connecting the proximal end 111 to the distal end 112, wherein the proximal end 111 forms an opening 111o for receiving the mouth 210 of the bottle 200 such that the mouth 210 can be inserted into the proximal end 111 to connect with the proximal end 111, and the distal end 112 forms an opening 112o intended to be covered by the cap 120. In particular, as shown in fig. 2, the inner surface of the proximal end 111 may be provided with internal threads that may be matched with external threads formed on the mouth 210 so that the bottle cap 100 may be screw-coupled to the mouth 210, and the outer surface of the proximal end 111 may be formed with engagement planes that may be engaged with a torque wrench so that the bottle cap 100 may be screwed to the mouth 210 by the torque wrench.

In particular, as shown in fig. 3 and 4, the cap body 110 may further include a stop ring 114 protruding radially inward from the inner surface of the proximal end 111, and the stop ring 114 may abut the mouth 210, thereby preventing the mouth 210 from moving further toward the cap 120, that is, preventing the mouth 210 from moving further toward the inside of the bottle cap 100. The above configuration is advantageous, and it should be noted that, firstly, the engagement area between the retainer ring 114 and the mouth 210 is an area where there is a possibility of leakage, the torque required to continue screwing the bottle cap 100 after the retainer ring 114 abuts the mouth 210 increases, so that by using a torque wrench, the torque required to screw the bottle cap 100 onto the mouth 210 can be controlled, and by screwing the bottle cap 100 onto the mouth 210 with a proper torque, a proper seal can be achieved between the retainer ring 114 and the mouth 210, thereby preventing leakage from occurring through the engagement area between the retainer ring 114 and the mouth 210, and also preventing excessive torque from screwing the bottle cap 100 onto the mouth 210, thereby protecting the bottle cap 100 and the mouth 210 from being damaged by excessive torque. In particular, a positioning mark may be provided on the cap body 110 or the cap 120 described later, and the positioning mark and the bottle body 200 may assume a specific relative position after the cap body 110 is screwed to a proper torque, so that, at the time of a subsequent inspection, it may be determined whether the cap body 110 is screwed to a proper torque through the relative position of the positioning mark and the bottle body 200, so as to determine whether a proper sealing between the bottle cap 100 and the bottle mouth 210 has been achieved.

In particular, as shown in fig. 3 and 4, the side of the stopper ring 114 facing the mouth 210 is formed with a groove that can receive the end of the mouth 210 and can receive an O-ring 115, and the O-ring 115 is pressed by the stopper ring 114 and the mouth 210 to be elastically deformed, and thus, seals the engagement area between the stopper ring 114 and the mouth 210. Due to the presence of the O-ring 115, leakage at the interface between the retainer ring 114 and the spout 210 is more reliably prevented.

In particular, as shown in fig. 2-4, the radial dimension (e.g., diameter or radius) of the proximal end 111 is less than the radial dimension of the distal end 112, which causes the intermediate portion 113 to have a funnel shape that extends radially inward or tapers as the proximal end 111 is approached, i.e., the intermediate portion 113 has a shape that tapers (in other words, tapers) from the distal end 112 toward the proximal end 111, which configuration is advantageous as described in further detail below in connection with the cap 120.

In particular, as shown in fig. 3 and 4, the distal end 112 is formed of two annular bodies that are radially spaced apart such that the distal end 112 is an annular structure having a U-shaped cross-section, which configuration is also advantageous as described in further detail below in connection with the cap 120.

The cap 120 is described in more detail below in conjunction with the drawings, and as shown in fig. 2-4, the cap 120 has a generally T-shaped structure including a cover plate 121 for covering the opening 112o of the distal end 112 of the cap body 110 and an inner tube 122 extending inwardly or proximally from the cover plate 121 (i.e., protruding from an inner surface of the cover plate 121), wherein the cover plate 121 may be, for example, heat-pressure welded, ultrasonically welded, or bonded to the distal end 112 of the cap body 110. As shown in particular in fig. 2 to 4, and in particular in the enlarged detail in fig. 3, the cover plate 121 is provided along its periphery with an annular structure having an inverted U-shaped section cooperating, more particularly mutually embedded, with the distal end 112 of the U-shaped section, so as to form a meandering joining region. This configuration is advantageous because the joint area of the cover plate 121 and the distal end 112, where leakage is likely to occur, can be more reliably avoided by arranging the joint area to have a meandering shape. The lumen of the inner tube 122 forms a cylindrical passage GC, and the cylindrical passage GC extends through the cover plate 121. In this configuration, after the bottle cap 100 is fixed to the mouth 210 of the bottle body 200, the inside of the bottle body 200 may communicate with the outside through the cylindrical passage GC, in other words, the inside of the bottle body 200 may be ventilated with the outside through the cylindrical passage GC, so as to prevent the pressure from being accumulated or a vacuum from being generated inside the bottle body 200. Therefore, the cylindrical passage GC may also be referred to as a gas passage GC.

In particular, as shown in fig. 2 and 3, the cap 120 may further include a hydrophobic gas-permeable membrane 123 covering the distal end of the gas channel GC, which hydrophobic gas-permeable membrane 123 may be, for example, heat-pressure welded, ultrasonically welded, or adhered to the cover plate 121. Since the hydrophobic vent membrane 123 may allow air to pass through but not liquid to pass through, it may be possible to prevent the liquid inside the bottle body 200 from leaking to the outside through the inner tube 122, and more particularly, the hydrophobic vent membrane 123 may also prevent microorganisms from passing through, and thus, it may also be possible to prevent the culture inside the bottle body 200 from being contaminated by the external microorganisms through the inner tube 122. In particular, as shown in fig. 2-4, the cap 120 may further include a protective cover 124 covering the hydrophobic vent membrane 123, the protective cover 124 may be provided with a plurality of vent holes and may be fixed to the cover plate 121, for example, in a snap-fit manner (as shown in fig. 3 and 4), in this configuration, the protective cover 124 may help to position the hydrophobic vent membrane 123 to prevent the hydrophobic vent membrane 123 from falling off accidentally, and may protect the hydrophobic vent membrane 123 from being accidentally damaged, and in addition, since the protective cover 124 is provided with a plurality of vent holes, the air permeability of the hydrophobic vent membrane 123 may not be affected.

In particular, as shown in fig. 3 and 4, the inner tube 122 extends from the cover plate 121 towards the proximal side or the interior to be inserted into the cover body 110 and the cover body 110 surrounds the inner tube 122, so that an annular channel (also referred to as an annular cavity) LC is defined between the inner tube 122 and the cover body 110, in other words, an annular channel LC surrounding the inner tube 122 is formed within the bottle cap 100, as described in further detail below, for communicating the interior of the bottle body 200 with the exterior liquid, and thus, the annular channel LC may also be referred to as a liquid channel LC, and as shown, the inner tube 122 spaces the liquid channel LC from the gas channel GC, thereby achieving gas-liquid separation, that is, the liquid in the liquid channel LC does not flow into the gas channel GC, and the gas in the gas channel GC does not flow into the liquid channel LC. In particular, the inner tube 122 extends proximally or inwardly into the proximal end 111, such that upon securing the bottle cap 100 to the mouth 210, the inner tube 122, the gas channel GC and the liquid channel LC will all extend into the mouth 210. More particularly, the inner tube 122 extends into the bottle body 200 (more particularly, the bottle body 220) through the mouth 210, such that the inner tube 122, the gas channel GC, and the liquid channel LC will all extend into the bottle body 200 after the bottle cap 100 is secured to the mouth 210. In this configuration, since the length of the inner tube 122 is long, the gas channel GC can be more reliably isolated from the liquid channel LC.

As shown in fig. 2 and 4, the cap 120 may further include an outer tube 125 extending distally or outwardly from the cover plate 121 (that is, protruding from a distal or outer surface of the cover plate 121), a lumen of the outer tube 125 forming an additional liquid channel LC ', and the additional liquid channel LC' extending through the cover plate 121. In addition, the outer tube 125 is located radially outside the inner tube 122, and thus, the additional liquid channel LC 'is open to the liquid channel LC after passing through the cap plate 121 (i.e., the additional liquid channel LC' communicates with the liquid channel LC at the inner surface of the cap plate 121, i.e., the inner surface of the cap 120), not to the gas channel GC. In this configuration, the liquid channel LC may communicate with the outside through the additional liquid channel LC ', and more particularly, after the bottle cap 100 is fixed to the mouth 210 of the bottle body 200, a culture medium may be injected into the liquid channel LC through the additional liquid channel LC ' to perform a fluid replacement operation, and a culture may also be taken out of the bottle body 200 through the additional liquid channel LC ' to perform a harvest operation, all without removing the bottle cap 100 from the mouth 210. Thus, this configuration also allows the use of a cell culture bottle in which the bottle cap 100 and the bottle body 200 are inseparably coupled or integrally manufactured, which can obviously more reliably prevent or even completely prevent leakage at the junction area between the bottle cap 100 and the bottle body 200, as compared to a cell culture bottle in which the bottle cap 100 and the bottle body 200 are detachably coupled.

As shown in fig. 2-4, the cap 120 may further include a hose 126 inserted into the outer tube 125 from a distal end of the outer tube 125, and in particular, a glue seal may be injected between the hose 126 and the outer tube 125 to prevent leakage at a junction area between the hose 126 and the outer tube 125. The lumen of the flexible tube 126 communicates with the lumen of the outer tube 125, i.e., the additional liquid channel LC ', so that, when a fluid replacement operation is performed, a culture medium can be injected into the flexible tube 126, the culture medium can enter the bottle body 200 along the flexible tube 126, the additional liquid channel LC ', and the liquid channel LC, and, when a harvest operation is performed, the culture in the bottle body 200 can exit the bottle body 200 along the liquid channel LC, the additional liquid channel LC ', and the flexible tube 126. It is worth mentioning that the fluid replacement operation and the harvesting operation are both time-consuming and short operations, that is, the cell culture flask is under the culturing operation most of the time, and the free end of the hose 126 is closed in order to prevent contaminants from entering the flask 200 through the hose 126 and prevent the liquid in the flask 200 from leaking to the outside through the hose 126. That is, the free end of the tube 126 is closed during the culturing of the mononuclear cells, and the tube 126 may be connected to other tubes through an aseptic tube connector so as to transfer the material into and out of the bottle 200 through the tube 126 using, for example, a peristaltic pump, during the fluid replacement operation and the harvesting operation. Under this configuration, the sterility requirements of the mononuclear cell culture can be met to the maximum extent and leakage is prevented. In particular, as shown in fig. 4, the cap 120 may include two sets of external pipes 125 and hoses 126, wherein one set of external pipes 125 and hoses 126 serves as a liquid inlet pipe set, and the other set of external pipes 125 and hoses 126 serves as a liquid outlet pipe set, and more particularly, a label such as "inlet" or "outlet" may be further provided on the cover plate 121 to help an operator distinguish the liquid inlet pipe set from the liquid outlet pipe set.

In particular, as shown in fig. 3 and 4, the O-ring 115 may be radially spaced from the liquid passage LC by the radially inner side of the stopper ring 114, and thus, the liquid in the liquid passage LC may be prevented from eroding the O-ring 115, thereby more reliably ensuring the sealing between the bottle cap 100 and the bottle mouth 210.

In particular, as shown in fig. 4, the lumen of the outer tube 125, i.e., the additional liquid channel LC', is axially aligned with the middle portion 113, and as described above, the middle portion 113 has a funnel shape. In this configuration, during the refilling operation, the liquid from the additional liquid channel LC' will flow onto the intermediate portion 113 so as to cause a reduction in the flow rate thereof, and then directed by the intermediate portion 113 toward the inner tube 122, whereupon the liquid will flow under inertia onto the outer surface of the inner tube 122, and flows into the bottle body 200 along the outer surface of the inner tube 122, so that the arrangement can make the liquid flow into the bottle body 200 more stably and uniformly, while the bottle cap 110 may be tilted downward during the harvesting operation, the liquid in the bottle body 200 will flow along the liquid channel LC toward the outer tube 125 instead of along the gas channel GC toward the hydrophobic gas-permeable membrane 123 due to the isolation of the inner tube 122, that is, the above arrangement allows the fluid to flow out of the bottle 200 more stably and uniformly even during the harvesting operation because the fluid flows along the reverse path to the fluid infusion operation.

Referring to fig. 5, which shows an exploded view of a bottle cap 100 of a cell culture bottle according to another embodiment of the present invention, the embodiment shown in fig. 5 has the same configuration as the embodiment shown in fig. 1 to 4 except that a flexible tube 126 is connected to an outer tube 125. As shown in fig. 5, the hose 126 may be fitted over the outer tube 125, or the outer tube 125 may be inserted into the hose 126, and the cap 120 may further include an elastic band 127 fitted over the outer tube 126 to bind the hose 126 to the outer tube 125. In particular, the outer tube 125 may be provided with an annular ridge 128 protruding radially from its outer surface. The annular ridge 128 may deform the hose 126 to a greater extent than other portions of the outer tube 125, thereby improving the seal at the interface between the outer tube 125 and the hose 126. More particularly, the elastic band 127 may be radially aligned with the annular ridge 128, which allows a greater degree of deformation of the elastic band 127 to occur as well, further improving the seal of the engagement area between the outer tube 125 and the hose 126.

The body 200 of the cell culture bottle according to an embodiment of the present invention will be described in detail with reference to fig. 1 to 4. As shown, the vial body 200 may generally include a mouthpiece 210 and a body 220 connected with the mouthpiece 210, the body 220 may have a pair of generally flat sidewalls, i.e., a first sidewall 221 and a second sidewall 222, opposite each other, which allows the cell culture flask to be smoothly placed on a support (e.g., a table or a console, etc.) in such a manner that the first sidewall 221 or the second sidewall 222 abuts against a surface of the support. In particular, the spout 210 extends from the body 220 in a direction away from the plane in which the first sidewall 221 lies, and more particularly, as seen in a cross-sectional view taken along a plane perpendicular to the first and second sidewalls 221 and 222 and also passing through the axis of the cylindrical spout 210, for example, in fig. 3, the side of the spout 210 proximate the second sidewall 222 extends from the second sidewall 222 in a direction away from the plane in which the first sidewall 221 lies. In this configuration, when the cell culture bottle is horizontally positioned with the first sidewall 221 against the support surface, the liquid within the bottle body 200 is deposited on the first sidewall 221 and the mouth 210 is above the first sidewall 221, which may prevent the liquid within the bottle body 200 from flowing into the mouth 210 for performing a culturing operation and/or a fluid replacement operation, whereas when the cell culture bottle is horizontally positioned with the second sidewall 222 against the support surface, the liquid within the bottle body 200 is deposited on the second sidewall 222 and a portion of the mouth 210 is below the second sidewall 222, which may facilitate the liquid within the bottle body 200 to flow into the mouth 210 for performing a harvesting operation.

Alternative but non-limiting embodiments of the cell culture flask according to the utility model are described in detail above with the aid of the accompanying drawings. Modifications and additions to the techniques and structures, as well as re-combinations of features in various embodiments, which do not depart from the spirit and substance of the disclosure, will be readily apparent to those of ordinary skill in the art as they are deemed to be within the scope of the utility model. Accordingly, such modifications and additions that can be envisaged within the teachings of the present invention are to be considered as part of the present invention. The scope of the present invention includes equivalents known at the time of filing and equivalents not yet foreseen.

Claims (10)

1. A cell culture bottle comprising a bottle cap (100) and a bottle body (200), the bottle cap (100) comprising a cap body (110) and a cap (120), the bottle body (200) comprising a mouth (210) and a body (220) in one piece, wherein,

the cap body (110) is connected at both ends to the mouth (210) and the cap (120), respectively, characterized in that,

the cap (120) is provided with an inner tube (122) on its inner surface, an outer tube (125) on its outer surface, and a flexible tube (126) connected to the outer tube (125), wherein the lumens of the inner tube (122) and the outer tube (125) both extend through the cap (120), the flexible tube (126) having a closed free end, and wherein an annular channel (LC) is formed around the inner tube (122), the annular channel (LC) communicating with the lumen of the outer tube (125).

2. The cell culture flask according to claim 1, wherein the annular channel (LC) communicates with the lumen of the outer tube (125) at the inner surface of the cap (120).

3. The cell culture flask according to claim 1, wherein the inner tube (122) and the annular channel (LC) extend through the mouth (210) into the body (220).

4. The cell culture flask according to claim 1, wherein the bottle cap (100) is integrated with the flask body (200).

5. The cell culture flask according to claim 1, wherein the body (220) has a first side wall (221) and a second side wall (222) that are planar and opposite to each other, wherein the mouth (210) extends away from the plane of the first side wall (221).

6. The cell culture flask according to claim 5, wherein, in a plane perpendicular to the first sidewall (221) and the second sidewall (222), a side of the mouth (210) close to the second sidewall (222) extends from the second sidewall (222) towards a direction away from the plane of the first sidewall (221).

7. The cell culture flask according to any of claims 1 to 6, wherein the flexible tube (126) is inserted into the outer tube (125) and the joint area of the flexible tube (126) and the outer tube (125) is sealed by glue injection.

8. The cell culture flask according to any one of claims 1 to 6, wherein the outer tube (125) is inserted into the flexible tube (126), and an elastic band (127) is provided around the outside of the flexible tube (126).

9. The cell culture flask according to claim 8, wherein the outer tube (125) is provided with an annular ridge (128) on its outer surface, the annular ridge (128) being inserted into the flexible tube (126).

10. The cell culture flask according to claim 9, wherein the elastic band (127) is aligned radially with the annular ridge (128).

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