CN208121101U - Small-sized fermentation tank - Google Patents
Small-sized fermentation tank Download PDFInfo
- Publication number
- CN208121101U CN208121101U CN201721325584.5U CN201721325584U CN208121101U CN 208121101 U CN208121101 U CN 208121101U CN 201721325584 U CN201721325584 U CN 201721325584U CN 208121101 U CN208121101 U CN 208121101U
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- CN
- China
- Prior art keywords
- tank body
- feed supplement
- rubber stopper
- fermentor
- turbid
- Prior art date
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- 238000000855 fermentation Methods 0.000 title claims abstract description 36
- 230000004151 fermentation Effects 0.000 title claims abstract description 36
- 239000006052 feed supplement Substances 0.000 claims abstract description 28
- 238000001514 detection method Methods 0.000 claims abstract description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000001301 oxygen Substances 0.000 claims abstract description 15
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 15
- 238000005276 aerator Methods 0.000 claims abstract description 12
- 239000000523 sample Substances 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 5
- 239000011521 glass Substances 0.000 claims description 11
- 238000005273 aeration Methods 0.000 claims description 8
- 229920000742 Cotton Polymers 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 241001052560 Thallis Species 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 abstract description 2
- 239000012531 culture fluid Substances 0.000 abstract 1
- 239000007789 gas Substances 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 238000011218 seed culture Methods 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000009529 body temperature measurement Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a kind of fermentor, including tank body（1）, tank body top center position feed opening（2）, surround equally distributed four detection mouths of feed opening（3）, identical with opacity tube internal diameter than turbid straight tube below tank body（4）, have the function of feeding simultaneously than turbid straight tube, than being furnished with the opacity tube of different turbidity beside turbid straight tube（13）, temp probe is respectively configured in four detection mouths（5）, pH electrode（6）, dissolved oxygen electrode（7）, aerator band air filter（8）, feed supplement pipe（9）, feed supplement pipe pumps by wriggling feed supplement（10）Carry out feed supplement, feed supplement pump connection feed supplement bottle（11）Gas bath device（12）Temperature control is carried out to tank body.The utility model has the advantages that：1. it is easy fermentation, it is easy to use.2. dismounting cleaning is simple.3. seed culture fluid can be provided for 100L fermentor.4. real-time monitoring and feed supplement can be carried out to temperature, pH value, dissolved oxygen, bacterium turbidity simultaneously.
Description
Technical field
The utility model relates to a kind of fermentors, and in particular to a kind of fermentor for bioengineered strain fermentation.
Background technique
Bio-engineering Products each field such as from food to medicine, for Bio-engineering Products mostly from fermentation, fermentation must not
The instrument that can lack is exactly fermentor, in large-scale production, improves yield top fermentation tank and plays an important role.Large fermentation tank body
Product is raised to upper kilolitre from tens, and large fermentation tank can detect each parameter of fermenting and regulate and control in time, but large fermentation tank is past
It is big toward volume, it is not easily shifted, it is expensive, need to configure professional fermentation personnel.Conical flask shaker fermentation is that laboratory is common
Fermentation method can obtain in short term a small amount of fermentation liquid, be unable to feed supplement, and dissolved oxygen and pH are unable to real-time detection and adjustment, fermentation volume
Less, the disadvantages of target product is few.Conical flask shaker fermentation and large fermentation tank fermentation exist very between each state modulator that ferments
Big difference, the utility model compensates for the few disadvantage of conical flask amount of fermentation, also economical and practical, easy to operate.Single fermentation
Available is more than the fermentation liquid of 8L, possesses aerator band air filter, can increase dissolved oxygen well.Tank design avoids
Dead angle, it is convenient for cleaning.Each detector of configuration can detect the other parameters such as pH simultaneously.It is a kind of convenience, economy, efficient small
Type fermentor.
Summary of the invention
The technical problem to be solved by the present invention is to：There is provided one kind can increase dissolved oxygen, to fermentation parameter on-line checking, can feed supplement
The convenient small-sized fermentation tank of economy.
The technical scheme is that：A kind of fermentor, including main tank body, pH electrode, dissolved oxygen electrode, aerator band are empty
Air filter, temp probe, wriggling feed supplement pump, air bath temperature control device, opacity tube.
Tank body material is glass, and with a thickness of 3 ~ 5mm, volume is 10 ~ 20L, and shape is ball-type, and flat, flat diameter is 100
~150mm.Glass transparent is easy to observe thalli growth situation, and thickness is moderate, keeps strength of glass to improve simultaneously and turns the thermal efficiency, ball
Type can be reduced dead angle in tank, flat that tank body can be made to keep stablizing.Feed opening is located at right above tank body, protrudes 100 ~ 150mm of tank body,
45 ~ 50mm of internal diameter is open as ground, and ground outer diameter is 50 ~ 55mm.Cotton plug is configured, extraneous miscellaneous bacteria is stopped to enter while keeping
Airing function.Detection mouth is four, is uniformly distributed around feed opening, and 100 ~ 150mm of tank body, the ground of bore 24/29mm are protruded.
Configuration cotton plug and rubber stopper, rubber stopper are divided into single hole rubber stopper and rubber stopper with double hole.It is high than the lower section that turbid straight tube is located at tank body
In flat 10 ~ 20mm, level protrudes 50 ~ 60mm, thickness 1mm, and 250 ~ 300mm of height clamps band tubing using tongs.Temperature
Degree probe, by single hole rubber stopper be inserted into detection mouth, 0 ~ 100 DEG C of temperature detection range, accuracy ± 1 degree.PH electrode, passes through list
Hole rubber stopper is inserted into detection mouth, detection range 1 ~ 14, accuracy 0.01.Dissolved oxygen electrode is inserted into detection mouth by single hole rubber stopper,
Detection range 1 ~ 100, accuracy 0.01.Aerator band air filter and feed supplement pipe, the pipeline of aeration tube and feed supplement pipe
Pipeline is inserted into rubber stopper with double hole simultaneously.Aerator band air filter is persistently aerated, and power uses aeration form in 0 ~ 1KW
Increase fermentation liquid oxygen content, instead of traditional conical flask shaker fermentation.Feed supplement range of pump flow rates is in 0.1 ~ 100mL/min.It mends
Expect bottle, body is glass, has rubber stopper with double hole, is resistant to high-temperature sterilization.Volume is in 50 ~ 1000mL.Air bath temperature control device will be sent out
Fermentation tank is completely packed into, and has the function of controlling fermentation jar temperature, and front is glass material, facilitates observation, thickness 5mm.Temperature control
4 ~ 60 DEG C of range, fluctuation range ± 1 DEG C.It is characterized in that：On-line checking can be carried out to fermentation pH, dissolved oxygen, temperature parameter simultaneously.
Eliminate the disturbing factor detected under line.It can ferment to aerobic bacteria, aeration stirring form substitutes blade stirring, can avoid silk
Winding of the shape bacterium to blade.Increase the quantity of peristaltic pump, while carrying out blowing and feed supplement, can continuously ferment.
Detailed description of the invention
Fig. 1 is schematic view of the front view of the invention.
Fig. 2 is fermentor top view.
Specific embodiment
Referring to Fig.1, fermentor of the invention, mainly by fermentation can body, mating pH electrode, dissolved oxygen electrode, temperature are visited
Head, peristaltic pump, aerator band air filter, air bath temperature control device, opacity tube composition.It is elucidated further below the present invention.
In conjunction with attached drawing as it can be seen that fermentor of the invention, is made of tank body and related necessary instrument.The specifically used mistake of the present invention
Journey is as follows：Fermentation medium is added by feed inlet, volume is no more than the 2/3 of tank body volume, beyond the Great Wall cotton plug.Aerator band
Air filter connects the pump line that can sterilize and is put into fermentor, and pump line connects rubber stopper, and rubber stopper clogs 24/29 ground, remains
Lower 24/29 ground is clogged with cotton plug.Hose than turbid straight tube is clamped with tongs, entire tank body（Equipped with culture medium）It is put into
Autoclave sterilizing, 121 DEG C of temperature, the time 30 minutes.It is cooled to room temperature after sterilizing.It is feed supplement pipe, aeration tube, single hole rubber stopper, double
Hole rubber stopper individually sterilizes, and 121 DEG C of temperature, the time 30 minutes.Supplemented medium sterilizing is configured, 121 DEG C of temperature, the time 30 divides
Clock.After the calibration of pH detecting electrode, with 75% ethyl alcohol degerming, it is inserted into single hole rubber stopper, single hole rubber stopper clogs detection mouth.Temperature
Degree probe is inserted into single hole rubber stopper, single hole rubber stopper clogs detection mouth using after 75% ethyl alcohol degerming.After dissolved oxygen electrode calibration,
Using 75% ethyl alcohol degerming, it is inserted into single hole rubber stopper, single hole rubber stopper clogs detection mouth.Feed supplement pipe is inserted into rubber stopper with double hole,
Aeration tube is inserted into the same rubber stopper with double hole, and rubber stopper with double hole clogs detection mouth.Using sterile working mode, by seed bacteria suspension
By in feed opening access culture medium.Whole device is put into air bath temperature control device, aerator is opened, adjusts aeration power.
Air bath temperature control device is opened, temperature control is carried out.Temperature-detecting device is opened, carries out temperature measurement, while carrying out to pH, dissolved oxygen
Detection, using opacity tube to reduced turbidity.Feed supplement pump is opened when necessary, and feed supplement is carried out to fermentation liquid.Also openable tongs sampling.
After fermentation, aerator, air bath temperature control device, pH electrode, dissolved oxygen electrode, temp probe, feed supplement pump are closed, fermentation is taken out
Tank takes pH electrode, dissolved oxygen electrode, temp probe, feed supplement pipe, aeration tube together with single hole rubber stopper and rubber stopper with double hole
Under.Tongs are opened, rubber tube is accessed in fermentation liquid collection vessel, collect fermentation liquid.It cleans fermentor and matches circuits.Hair
Ferment terminates.
Claims (12)
1. fermentor, including tank body（1）, tank body top center position feed opening（2）, four detections are uniformly distributed around feed opening
Mouthful（3）, tank body lower section has identical with opacity tube internal diameter than turbid straight tube（4）, have the function of feeding simultaneously than turbid straight tube, it is more straight than turbid
It is furnished with the opacity tube of different turbidity beside pipe（15）, four detection mouths（3）Temp probe is respectively configured（5）, pH electrode（6）, molten
Oxygen electrode（7）, aerator（82）Band air filter（83）, feed supplement pipe（9）, feed supplement pipe pumps by wriggling feed supplement（10）It is mended
Material, feed supplement pump connection feed supplement bottle（11）, air bath temperature control device（12）Temperature control is carried out to tank body.
2. fermentor according to claim 1, it is characterised in that：The tank body（1）Material is glass or organic glass,
With a thickness of 3 ~ 5mm, volume is 10 ~ 20L, and shape is ball-type, and flat, flat diameter is 100 ~ 150mm, and glass transparent is easy to observe
Thalli growth situation, thickness is moderate, keeps strength of glass to improve simultaneously and turns the thermal efficiency, ball-type can be reduced dead angle in tank, flat energy
Tank body is set to keep stablizing.
3. fermentor according to claim 1, it is characterised in that：The feed opening（2）Right above tank body, protrusion
100 ~ 150mm of tank body, 45 ~ 50mm of internal diameter are open as ground, and ground outer diameter is 50 ~ 55mm, configure cotton plug（21）, stop outer
Boundary miscellaneous bacteria enters while keeping airing function.
4. fermentor according to claim 1, it is characterised in that：The detection mouth（3）It is four, surrounds feed opening
（2）It is uniformly distributed, protrudes 100 ~ 150mm of tank body, the ground of bore 24/29mm, configures cotton plug and rubber stopper, rubber stopper are divided into
Single hole rubber stopper and rubber stopper with double hole.
5. fermentor according to claim 1, it is characterised in that：The turbid straight tube of ratio（4）Positioned at tank body（1）Under
Side, is higher than flat 10 ~ 20mm, and level protrudes 50 ~ 60mm, thickness 1mm, 250 ~ 300mm of height, than turbid straight tube（4）The other end
Band tubing is connected, tongs are used（13）Clamp band tubing.
6. fermentor according to claim 1, it is characterised in that：The temp probe（5）, inserted by single hole rubber stopper
Enter detection mouth（3）, 0 ~ 100 DEG C of temperature detection range, accuracy ± 1 DEG C.
7. fermentor according to claim 1, it is characterised in that：The pH electrode（6）, it is inserted by single hole rubber stopper
Detection mouth（3）, detection range 1 ~ 14, accuracy ± 0.01.
8. fermentor according to claim 1, it is characterised in that：The dissolved oxygen electrode（7）, inserted by single hole rubber stopper
Enter detection mouth（3）, detection range 1 ~ 100, accuracy ± 0.01.
9. fermentor according to claim 1, it is characterised in that：The aerator（82）Aeration tube（81）And benefit
Expects pipe（9）It is inserted into rubber stopper with double hole simultaneously, rubber stopper with double hole is inserted into detection mouth（3）, aerator（82）With air filter
（83）, persistently it is aerated in use, power is in 0 ~ 1KW, feed supplement pump（10）Range of flow is in 0.1 ~ 100mL/min.
10. fermentor according to claim 1, it is characterised in that：The feed supplement bottle（11）, body is glass, is had
Rubber stopper with double hole is resistant to high-temperature sterilization, feed supplement bottle（11）Volume is in 50 ~ 1000mL.
11. fermentor according to claim 1, it is characterised in that：The air bath temperature control device（12）Fermentor is complete
Whole loading has the function of control fermentation jar temperature, and front is glass material, and thickness 5mm, is fluctuated by 4 ~ 60 DEG C of temperature-control range
Range ± 1 DEG C.
12. fermentor according to claim 1, it is characterised in that：The opacity tube（15）Positioned at air bath temperature control device
（12）Inner wall, position with than turbid straight tube（4）It is adjacent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721325584.5U CN208121101U (en) | 2017-10-16 | 2017-10-16 | Small-sized fermentation tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721325584.5U CN208121101U (en) | 2017-10-16 | 2017-10-16 | Small-sized fermentation tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208121101U true CN208121101U (en) | 2018-11-20 |
Family
ID=64208192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721325584.5U Active CN208121101U (en) | 2017-10-16 | 2017-10-16 | Small-sized fermentation tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208121101U (en) |
-
2017
- 2017-10-16 CN CN201721325584.5U patent/CN208121101U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Small fermentation tank Effective date of registration: 20231214 Granted publication date: 20181120 Pledgee: Societe Generale Limited by Share Ltd. Chongqing branch Pledgor: CHONGQING XIERKANG BLOOD PURIFICATION EQUIPMENT RESEARCH DEVELOPMENT CO.,LTD. Registration number: Y2023500000103 |