CN101278646A - Method for producing sterilized miniascape - Google Patents
Method for producing sterilized miniascape Download PDFInfo
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- CN101278646A CN101278646A CNA2008100711313A CN200810071131A CN101278646A CN 101278646 A CN101278646 A CN 101278646A CN A2008100711313 A CNA2008100711313 A CN A2008100711313A CN 200810071131 A CN200810071131 A CN 200810071131A CN 101278646 A CN101278646 A CN 101278646A
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- miniascape
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- Y02P60/216—
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A method for manufacturing an asepsis bonsai relates to a manufacture method of an ornamental gardening bonsai; that is, an aseptic seedling cultivated by isolated culture and decorative material are placed in an aseptic transparent container; as the water and nutrient are provided by a special culture medium, the aseptic seedling in an environment isolated from the outside can gradually grow and even blossom. Only by being placed in an environment with the normal temperature and under common scattered light, the asepsis bonsai can grow and develop in the transparent artware bottle container. People can observe the whole process of rootage, germination and blossoming. The aseptic seedling does not have any diseases and insect pests, does not need daily maintenance, and is environment-friendly, sanitary and beautiful; the ornamental period reaches more than 6 months. The asepsis bonsai not only is a recreation item, but also can help middle and primary school students recognize and understand plants.
Description
Technical field
The present invention relates to a kind of preparation method of ornamental horticulture potted landscape, particularly aseptic seedling and the ornament materials with cultured in vitro places in the aseptic transparent container, and aseptic seedling is grown in the environment that is isolated from the outside or the preparation method of the sterilized miniascape of blooming.
Background technology
Sterilized miniascape is to utilize germ-free plant and ornament to form the handicraft of various miniature views in container.Advantages such as it need not any maintenance, has high novelty, and is elegant in appearance.Be a kind of art work of elegance, have the good prospect of marketing.
The kind of existing cultured in vitro test-tube plantlet success is a lot, and the kind of blooming in the blake bottle also has tens kinds.Wherein some kind flowering time in bottle is short, and some kind flowering rate is very low.Some kind test tubes flower is not had an ornamental value.So, really in bottle, to bloom, the florescence is long, the flowering rate height, the kind that sight is strong is few.The mini rose of Korea S also has only about one month flowering stage.Yet there are no report in aseptic container, making potted landscape.Only see resin solid contents such as in open container, adding quartzy mud, and plant is planted into wherein, make its short time survival.Owing to be open cultivation, will inevitably cause the evaporation of moisture, time length will be smelly, causes root mashed dead, so also need a mouthful normal management, this also is that quartzy mud potted flower is difficult to promote the one of the main reasons of opening on market.
Summary of the invention
The objective of the invention is to make sterilized miniascape, promptly aseptic seedling and various ornament materials are made into various sterilized miniascapes, put sterilized miniascape in aseptic transparent container, make in the environment that aseptic seedling is isolated from the outside, provide moisture and nutrient by special medium, gradually grow up, even bloom.
The preparation method of sterilized miniascape of the present invention comprises explant sterilization, medium preparation and potted landscape field planting, specifically comprises following steps:
1, explant sterilization: identical with the Plant Tissue Breeding routine disinfection.Explant after the washing powder surface clean, was soaked 30 seconds with 75% alcohol, and 0.1% mercuric chloride soaked 5-20 minute, 3-5 back inoculation of sterile water wash.Described explant such as seed, leaf bud, blade ...
2, medium preparation: according to culture medium prescription, take by weighing agar powder, white sugar earlier, adds boil in 300 milliliters the distilled water after, number adds other compositions in filling a prescription for another example; Increase the color of medium if desired, can also add various food colorings, the use total amount of pigment is no more than 80mg/L; More than the dissolving of various compositions mix back adding distil water to 1000 milliliter, transfer to pH5.8 with 3% NaOH or HCl again; Then, the medium branch is installed in the transparent vessel seal.Sterilization is 20 minutes under the 1.1kg/cm high pressure, and is standby after the cooled and solidified.Various compositions and consumption are as follows in the described medium:
Sequence number composition title consumption mg/L sequence number composition title consumption mg/L
1 KNO
3500～950 15 nicotinic acid 0.5
2 NH
4NO
3300～500 16 pyridoxine hydrochlorides 0.5
3 Mg (SO)
47H
2O 185～370 17 thiamine hydrochlorides 0.1
4 KH
2PO
385～170 18 inositols 100
5 CaCl
2.2H
2O 220～440 19 glycine 2
6 FeSO
47H
2O 27.8 20 white sugar 20000
7 Na
2-EDTA 37.3 21 agar powders 6000
8 MnSO
4.4H
2The misty purine 0.1～3 of O 22.3 22 6-benzyl glands
9 ZnSO
4.7H
2O 8.6 23 methyls 0.1～3
10 CoCl
2.6H
2O 0.025 24 15% paclobutrazol 0.01～5
11 CuSO
4.5H
2O 0.025 25 spermidine 0～5
12 NaMoO
4.2H
2O 0.25
13 KI 0.83
14 H
3BO
3 6.2
Above chemical reagent is drying solid thing, chemical pure; Mg/L represents the milligram number of contained medicine in 1 liter of medium.Wherein, the misty purine of 6-benzyl gland dissolves with 1N HCI.Methyl dissolves with 95% alcohol.
3, aseptic cultivation: the aseptic seedling and the ornament materials of sterilizing are inoculated in the transparent container under aseptic condition, seal then.Described joint filling material, but as preservative film or seal film or the material of disinfection filtering air, Described ornament materials: as artificial hillock, coral; The tower that pottery is done, booth, foot bridge, small potato, various animal; The bridge that plastics are done, flag, various personage ...The ornament materials autoclave sterilization that artificial hillock, coral and pottery are done, the ornament materials that plastics are done use after sterilizing with chemosterilant.
The technology of the present invention has following beneficial effect:
1. culture medium prescription of the present invention is compared with the MS medium, and its N content reduces, and P, K content improve.Help the flower bud differentiation of plant, promote to bloom (as shown in table 1).Misty purine of 6-benzyl gland and methyl are to promote plant cell division, differentiation.15% paclobutrazol can be controlled the size of plant and promote early flowering (as shown in table 2).Spermidine is the flower bud differentiation of promotion plant and blooms.
Food coloring is increase medium attractive in appearance.
The influence of the different medium of table 1 to blooming in the pinniform cockscomb vase
| Medium | Inoculation bud number | The strain number of blooming | Flowering rate (%) |
| MS | 60 | 10 | 16.7 |
| 1/2MS | 60 | 13 | 21.7 |
| Medium of the present invention | 60 | 54 | 90.0 |
Annotate: use medium of the present invention to compare with the 1/2MS medium with MS, flowering rate all is significantly improved, and flowering rate reaches 90%.
The influence of the different paclobutrazol concentration of table 2 to blooming in the pinniform cockscomb vase
*
| 15% paclobutrazol (mg/L) | Inoculation bud number (individual) | The strain number (strain) of blooming | Flowering rate (%) | Flowering time (my god) | Average plant height (cm) |
| 0 | 60 | 49 | 81.3 | 48 | 2.7 |
| 0.5 | 60 | 53 | 88.3 | 35 | 1.5 |
| 1 | 60 | 54 | 90.0 | 30 | 1.3 |
| 2 | 60 | 48 | 80.0 | 34 | 0.9 |
*Medium is a medium of the present invention, except the paclobutrazol difference; Flowering time is from being seeded into the bud time of occurrence.Result of the test shows: along with the concentration of paclobutrazol raises, the height of plant obviously reduces, and the time of blooming but has significantly and shifts to an earlier date.Concerning flowering rate, the optium concentration of paclobutrazol is 0.5mg/L and 1.0mg/L, and flowering rate is all more than 88.0%.
2. sterilized miniascape of the present invention only need be placed under normal temperature environment and the general scattered light, any damage by disease and insect can not occur, need not any maintenance usually, environmental protection, health, attractive in appearance.Viewing period is more than 6 months.
3. sterilized miniascape of the present invention is to produce under aseptic, controlled environment, is not subjected to the restriction of natural conditions, and the anniversary can produce.
4. sterilized miniascape of the present invention absorbs abundant nutrient medium in aseptic, transparent art bottle container, it is grown, even bloom.People can observe the taking root of plant, rudiment, bloom etc. in appreciation.The extracurricular activities project that this can be used as students in middle and primary schools allows them be familiar with plant, understands plant.
5. sterilized miniascape of the present invention is compared with present technology, and the most outstanding a bit narrows down to penjing art and art of landscape in aseptic, the transparent art bottle container exactly, and people can produce the various miniature arts work according to the design of oneself.This also provides an application space widely for biological technology industry.
6. this technology is high in technological content, and market prospects are good, is convenient to industrialization production.
Description of drawings:
Fig. 1 is the ornamental foliage plant sterilized miniascape, and the plant among the figure is red cage grass;
Fig. 2 is a kind of mixing sterilized miniascape, and the plant among the figure is for closing fruit taro, acacia rachii and cockscomb.
Specific embodiment:, be illustrated below in conjunction with embodiment in order fully to disclose the preparation method of sterilized miniascape of the present invention.
Example 1: a kind of ornamental foliage plant sterilized miniascape (described ornamental foliage plant such as red cage grass, close the fruit taro, oncidiumLuridum, dendrobium, Caladium bicolor are arranged ...), its preparation method is as follows:
1, explant sterilization: the explant (comprising seed, leaf bud, blade etc.) of plant after the washing powder surface clean, was soaked 30 seconds with 75% alcohol, and 0.1% mercuric chloride soaked 5-20 minute, 3-5 back inoculation of sterile water wash;
2, preparation medium: according to prescription, take by weighing agar powder, white sugar earlier, join boil in 300 milliliters the distilled water after, number adds other compositions in the prescription for another example, other adds red food coloring 3mg/L; More than the dissolving of various compositions mix back adding distil water to 1000 milliliter, again with behind 3% the NaOH or 1N HCl accent pH to 5.8, the medium branch installed in the transparent vessel seals.At 1.1kg/cm
2High pressure was sterilized 20 minutes down, and is standby after the cooled and solidified.Can be simultaneously during autoclaving with the ornament materials autoclaving of high temperature high voltage resistant.Described culture medium prescription is as follows:
Sequence number composition title consumption mg/L sequence number composition title consumption mg/L
1 KNO
3950 15 nicotinic acid 0.5
2 NH
4NO
3450 16 pyridoxine hydrochlorides 0.5
3 Mg (SO)
47H
2O 185 17 thiamine hydrochlorides 0.1
4 KH
2PO
385 18 inositols 100
5 CaCl
2.2H
2O 220 19 glycine 2
6 FeSO
47H
2O 27.8 20 white sugar 20000
7 Na
2-EDTA 37.3 21 agar powders 6000
8 MnSO
4.4H
2The misty purine 0.1 of O 22.3 22 6-benzyl glands
9 ZnSO
4.7H
2O 8.6 23 methyls 0.5
10 CoCl
2.6H
2O 0.025 24 15% paclobutrazol 0.01
11 CuSO
4.5H
2O 0.025 25 spermidine 0
12 NaMoO
4.2H
2O 0.25
13 KI 0.83
14 H
3BO
3 6.2
3. aseptic cultivation: according to shape, the size of container, the aseptic seedling that selection and container adapt, and ornament materials.According to the design that will make potted landscape is located, the ornament materials after will sterilizing earlier was placed in before also not solidifying fully behind the medium high pressure on the medium of design opposite position on clean work station.After medium solidifies fully, various aseptic seedling are inoculated on the medium of relevant position in the container, with bottle cap or fresh-keeping approaching transparent vessel are sealed at last.
As shown in Figure 1, a kind of ornamental foliage plant sterilized miniascape under room temperature and natural scattering light, just can be enjoyed the process of growth of plant, and viewing period can be more than 6 months.
Example 2: a kind of preparation method of decorative flower sterilized miniascape may further comprise the steps: (described decorative flower is as cockscomb, China pink, morning glory ...):
Step 1 and step 3 and embodiment one roughly the same, the method for preparation medium is as follows in the step 2:
According to culture medium prescription, take by weighing agar powder, white sugar earlier, join boil in 300 milliliters the distilled water after, number adds other compositions in the prescription for another example; Other adds lemon yellow food coloring 60mg/L; More than the dissolving of various compositions mix back adding distil water to 1000 milliliter, again with behind 3% the NaOH or 1N HCl accent PH to 5.8, the medium branch installed in the transparent vessel seals.At 1.1kg/cm
2High pressure was sterilized 20 minutes down, and is standby after the cooled and solidified.Can be simultaneously during autoclaving with the ornament materials autoclaving of high temperature high voltage resistant.Described culture medium prescription is as follows:
Sequence number composition title consumption mg/L sequence number composition title consumption mg/L
1 KNO
3500 15 nicotinic acid 0.5
2 NH
4NO
3300 16 pyridoxine hydrochlorides 0.5
3 Mg (SO)
47H
2O 370 17 thiamine hydrochlorides 0.1
4 KH
2PO
3170 18 inositols 100
5 CaCl
2.2H
2O 220 19 glycine 2
6 FeSO
47H
2O 27.8 20 white sugar 20000
7 Na
2-EDTA 37.3 21 agar powders 6000
8 MnSO
4.4H
2The misty purine 0.1 of O 22.3 22 6-benzyl glands
9 ZnSO
4.7H
2O 8.6 23 methyls 1
10 CoCl
2.6H
2O 0.025 24 15% paclobutrazol 3
11 CuSO
4.5H
2O 0.025 25 spermidine 1
12 NaMoO
4.2H
2O 0.25
13 KI 0.83
14 H
3BO
3 6.2
Example 3: a kind of mixing sterilized miniascape (described mixing sterilized miniascape, as shown in Figure 2, to close the really potted landscape of the aseptic seedling combined inoculation of taro, acacia rachii and cockscomb), preparation method may further comprise the steps:
Step 1 and step 3 and embodiment one roughly the same, the method for preparation medium is as follows in the step 2:
According to culture medium prescription, take by weighing agar powder, white sugar earlier, join boil in 300 milliliters the distilled water after, number adds other compositions in the prescription for another example; Other adds blue look food coloring 3mg/L, (perhaps do not add pigment can) yet, more than various compositions dissolvings mix back adding distil water to 1000 milliliter, again with behind 3% the NaOH or 1N HCl accent pH to 5.8, the medium branch installed in the transparent vessel seals.At 1.1kg/cm
2High pressure was sterilized 20 minutes down, and is standby after the cooled and solidified.Can be simultaneously during autoclaving with the ornament materials autoclaving of high temperature high voltage resistant.Described culture medium prescription is as follows:
Sequence number composition title consumption mg/L sequence number composition title consumption mg/L
1 KNO
3500 15 nicotinic acid 0.5
2 NH
4NO
3300 16 pyridoxine hydrochlorides 0.5
3 Mg (SO)
47H
2O 370 17 thiamine hydrochlorides 0.1
4 KH
2PO
3170 18 inositols 100
5 CaCl
2.2H
2O 220 19 glycine 2
6 FeSO
47H
2O 27.8 20 white sugar 20000
7 Na
2-EDTA 37.3 21 agar powders 6000
8 MnSO
4.4H
2The misty purine 0.5 of O 22.3 22 6-benzyl glands
9 ZnSO
4.7H
2O 8.6 23 methyls 2
10 CoCI
2.6H
2O 0.025 24 15% paclobutrazol 1
11 CuSO
4.5H
2O 0.025 25 spermidine 2
12 NaMoO
4.2H
2O 0.25
13 KI 0.83
14 H
3BO
3 6.2
Claims (5)
1, a kind of preparation method of sterilized miniascape comprises explant sterilization, medium preparation and aseptic cultivation, it is characterized in that
A, described culture medium preparation method according to culture medium prescription, take by weighing agar powder, white sugar earlier, add boil in 300 milliliters the distilled water after, number adds other compositions in the prescription for another example; Increase the color of medium if desired, can also add various food colorings in addition, the use total amount of pigment is no more than 80mg/L; More than the dissolving of various compositions mix back adding distil water to 1000 milliliter, transfer to pH5.8 with 3% NaOH or HCl again; Then, the medium branch is installed in the transparent vessel seal.Sterilization is 20 minutes under the 1.1kg/cm high pressure, and is standby after the cooled and solidified;
Various compositions and consumption are as follows in B, the described medium:
Sequence number composition title consumption mg/L sequence number composition title consumption mg/L
1 KNO
3500～950 15 nicotinic acid 0.5
2 NH
4NO
3300～500 16 pyridoxine hydrochlorides 0.5
3 Mg (SO)
47H
2O 185～370 17 thiamine hydrochlorides 0.1
4 KH
2PO
385～170 18 inositols 100
5 CaCl
2.2H
2O 220～440 19 glycine 2
6 FeSO
47H
2O 27.8 20 white sugar 20000
7 Na
2-EDTA 37.3 21 agar powders 6000
8 MnSO
4.4H
2The misty purine 0.1～3 of O 22.3 22 6-benzyl glands
9 ZnSO
4.7H
2O 8.6 23 methyls 0.1～3
10 CoCl
2.6H
2O 0.025 24 15% paclobutrazol 0.01～5
11 CuSO
4.5H
2O 0.025 25 spermidine 0～5
12 NaMoO
4.2H
2O 0.25
13 KI 0.83
14 H
3BO
3 6.2
C, described aseptic cultivation: the aseptic seedling and the ornament materials of sterilizing are inoculated in the transparent container under aseptic condition, seal then.
2, the preparation method of a kind of sterilized miniascape according to claim 1 is characterized in that adding various food colorings in medium.
3, the preparation method of a kind of sterilized miniascape according to claim 1 and 2 is characterized in that various compositions and consumption are as follows in the described medium:
Sequence number composition title consumption mg/L sequence number composition title consumption mg/L
1 KNO
3500 15 nicotinic acid 0.5
2 NH
4NO
3300 16 pyridoxine hydrochlorides 0.5
3 Mg (SO)
47H
2O 370 17 thiamine hydrochlorides 0.1
4 KH
2PO
3170 18 inositols 100
5 CaCI
2.2H
2O 220 19 glycine 2
6 FeSO
47H
2O 27.8 20 white sugar 20000
7 Na
2-EDTA 37.3 21 agar powders 6000
8 MnSO
4.4H
2The misty purine 0.1 of O 22.3 22 6-benzyl glands
9 ZnSO
4.7H
2O 8.6 23 methyls 1
10 CoCI
2.6H
2O 0.025 24 15% paclobutrazol 3
11 CuSO
4.5H
2O 0.025 25 spermidine 1
12 NaMoO
4.2H
2O 0.25
13 KI 0.83
14 H
3BO
3 6.2
4, the preparation method of a kind of sterilized miniascape according to claim 2 is characterized in that the use total amount of pigment is no more than 80mg/L.
5, the preparation method of a kind of sterilized miniascape according to claim 1, but it is characterized in that joint filling material is preservative film or seals film or the material of disinfection filtering air.
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|---|---|---|---|
| CN2008100711313A CN101278646B (en) | 2008-05-26 | 2008-05-26 | Method for producing sterilized miniascape |
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|---|---|---|---|
| CN2008100711313A CN101278646B (en) | 2008-05-26 | 2008-05-26 | Method for producing sterilized miniascape |
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|---|---|
| CN101278646A true CN101278646A (en) | 2008-10-08 |
| CN101278646B CN101278646B (en) | 2010-08-18 |
Family
ID=40011467
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| CN2008100711313A Expired - Fee Related CN101278646B (en) | 2008-05-26 | 2008-05-26 | Method for producing sterilized miniascape |
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| CN103858760A (en) * | 2014-02-26 | 2014-06-18 | 福建省农业科学院作物研究所 | Manufacturing method of test-tube type anoectohilus formosanus |
| CN104822256A (en) * | 2012-10-11 | 2015-08-05 | 瑞克斯旺种苗集团公司 | Vegetables, mushrooms or herbs in box |
| CN106234215A (en) * | 2016-07-29 | 2016-12-21 | 韩山师范学院 | A kind of colorful noctilucent culture medium and its preparation method and application is in miniature potted landscape |
| CN106888950A (en) * | 2017-02-23 | 2017-06-27 | 朱家伟 | A kind of agarose gel matrix and preparation method thereof |
| CN107556119A (en) * | 2017-10-24 | 2018-01-09 | 山西梅芝园艺有限公司 | A kind of special nutrient fluid suitable for bottle plant thing |
| WO2018068281A1 (en) * | 2016-10-14 | 2018-04-19 | 泉州市泉美生物科技有限公司 | Method for cultivating plant in transparent sealed container and medium therefor |
| CN111642380A (en) * | 2019-12-03 | 2020-09-11 | 南阳师范学院 | Process flow of micro landscape artwork in test tube |
| CN112042536A (en) * | 2020-09-09 | 2020-12-08 | 北京花乡花木集团有限公司 | Construction method of tissue culture in-bottle micro landscape and tissue culture in-bottle micro landscape |
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| CN1214703C (en) * | 1999-11-05 | 2005-08-17 | 付嘉林 | Production technology of packed potted flowers and trees |
| CN1473457A (en) * | 2002-09-20 | 2004-02-11 | 杭州泛邦植物细胞工程有限公司 | Aseptic prouduction process for medicinal plant fresh product |
| CN1176576C (en) * | 2003-04-28 | 2004-11-24 | 陕西师范大学 | Australian greening (Grany Smith) quick propagation method |
| CN100367846C (en) * | 2005-04-30 | 2008-02-13 | 浙江理工大学 | Highly effective revulsion induction method for pinellia tuber excised tuber |
| CN1806528A (en) * | 2006-02-13 | 2006-07-26 | 陈曦 | Sterile culture method living plant artwork |
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2008
- 2008-05-26 CN CN2008100711313A patent/CN101278646B/en not_active Expired - Fee Related
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| CN104822256A (en) * | 2012-10-11 | 2015-08-05 | 瑞克斯旺种苗集团公司 | Vegetables, mushrooms or herbs in box |
| CN103858760A (en) * | 2014-02-26 | 2014-06-18 | 福建省农业科学院作物研究所 | Manufacturing method of test-tube type anoectohilus formosanus |
| CN103858760B (en) * | 2014-02-26 | 2016-04-13 | 福建省农业科学院作物研究所 | The preparation method of a kind of roxburgh anoectochilus terminal bud test tube flowers |
| CN106234215A (en) * | 2016-07-29 | 2016-12-21 | 韩山师范学院 | A kind of colorful noctilucent culture medium and its preparation method and application is in miniature potted landscape |
| WO2018068281A1 (en) * | 2016-10-14 | 2018-04-19 | 泉州市泉美生物科技有限公司 | Method for cultivating plant in transparent sealed container and medium therefor |
| RU2695449C1 (en) * | 2016-10-14 | 2019-07-23 | Саншайн Хортикултуре Ко., Лтд. | Plant cultivation method in transparent sealed container |
| CN106888950A (en) * | 2017-02-23 | 2017-06-27 | 朱家伟 | A kind of agarose gel matrix and preparation method thereof |
| CN107556119A (en) * | 2017-10-24 | 2018-01-09 | 山西梅芝园艺有限公司 | A kind of special nutrient fluid suitable for bottle plant thing |
| CN111642380A (en) * | 2019-12-03 | 2020-09-11 | 南阳师范学院 | Process flow of micro landscape artwork in test tube |
| CN112042536A (en) * | 2020-09-09 | 2020-12-08 | 北京花乡花木集团有限公司 | Construction method of tissue culture in-bottle micro landscape and tissue culture in-bottle micro landscape |
| CN112335547A (en) * | 2020-11-11 | 2021-02-09 | 泉州市泉美生物科技有限公司 | Cultivation method of monarch tissue culture seedling micro-vase landscape |
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|---|---|
| CN101278646B (en) | 2010-08-18 |
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