CN111642380A - Process flow of micro landscape artwork in test tube - Google Patents
Process flow of micro landscape artwork in test tube Download PDFInfo
- Publication number
- CN111642380A CN111642380A CN201911219602.5A CN201911219602A CN111642380A CN 111642380 A CN111642380 A CN 111642380A CN 201911219602 A CN201911219602 A CN 201911219602A CN 111642380 A CN111642380 A CN 111642380A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- sterile
- test tube
- micro
- artwork
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B44—DECORATIVE ARTS
- B44C—PRODUCING DECORATIVE EFFECTS; MOSAICS; TARSIA WORK; PAPERHANGING
- B44C5/00—Processes for producing special ornamental bodies
- B44C5/06—Natural ornaments; Imitations thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a process flow of micro landscape artwork in a test tube, which relates to the technical field of ornamental horticulture and can be used for producing the artwork with a certain theme and consistent effect in batch. The method comprises the following steps: step 1: obtaining aseptic seedlings; step 2: preparing a color culture medium; and step 3: pouring the sterilized and cooled color culture medium into sterile test tubes, and subpackaging each sterile test tube with a culture medium which occupies 1/4-1/3 to obtain a micro culture medium; and 4, step 4: carrying out pattern design of a plan view and an elevation view; and 5: manufacturing the micro landscape in the test tube, and inoculating the sterile seedlings and the matched plant materials with set quantity and height at a set position by using sterile forceps according to a plan view; according to the elevation map, adjusting the height of the plant to finish the landscaping of the plant; the test tube is sealed to form the test tube microlandscape artwork.
Description
Technical Field
The invention relates to the technical field of ornamental horticulture, in particular to a process flow of a micro landscape artwork in a test tube.
Background
The isolated plant micro-landscape refers to a micro-artistic landscape with unique ornamental value, which is finally formed by miniaturizing plants with ornamental value through an isolated culture technology, landscaping in a sterile container by combining an artistic method with natural beauty of the plants and matching culture media and accessories with different colors.
The landscape art in the prior art has the problems of difficult in-vitro preservation, poor consistency of art products in production and the like. The diversity and freedom of plant growth, and the specificity of isolated plants, are prone to the problem of inconsistent artwork effects from the same subject during mass production.
Disclosure of Invention
One object of the present invention is to provide a process for the micro landscape artwork in test tubes, so as to mass produce artwork with a certain theme and consistent effect.
Particularly, the invention provides a process flow of micro landscape artwork in a test tube, which comprises the following steps:
step 1: obtaining landscape plant aseptic seedlings which are cultured for multiple generations and stably proliferated in an increment way;
step 2: preparing a color culture medium;
and step 3: sterilizing the prepared color culture medium by high-pressure steam, pouring the sterilized and cooled color culture medium into sterile test tubes, subpackaging each sterile test tube with the culture medium occupying the height of 1/4-1/3, blowing off excessive water vapor on a super clean bench, and covering a cover when the culture medium is completely cooled and solidified to obtain a micro culture medium;
and 4, step 4: carrying out pattern design of a plan view and an elevation view, wherein the diameter of the plan view is the diameter of the micro culture medium, and the plan view contains position information, type information and quantity information of plants; the height of the elevation is 1/2-4/5 of the height of the micro culture medium, and the elevation contains height information and plant type requirements of plants;
and 5: manufacturing an in-vitro microscape, and placing the plane picture below the micro culture medium; according to the schematic of the plan, inoculating the sterile seedlings and the matched plant materials with set quantity and height at the set position by using sterile tweezers for plant cultivation; according to the display effect of the elevation map, adjusting the height of the plant to finish the plant landscaping; and sealing the test tube to ensure that the product is sterile so as to form the test tube micro landscape artwork.
Preferably, the step 1 specifically comprises the following steps:
step 101: taking annual branches of healthy, disease and insect pest-free adult landscaping plants with typical variety characteristics planted in fields;
step 102: and (3) explant disinfection treatment: removing skin thorn and leaf on the branch, shearing 1-1.5 cm stem with bud, washing with running water for 2h, submerging explant with 75% alcohol in a super clean bench, shaking for 40s, and using 0.1% HgCl2Soaking in the solution for 5min, washing with sterile water for 3 times, and drying with sterile filter paper;
step 103: obtaining and proliferating aseptic seedlings: inoculating the treated stem segments of the landscaping plants to a sterile bud induction culture medium, wherein the induction culture medium is MS + 3% sucrose +8g/L agar powder, one explant is inoculated in each bottle, and after 10 days, the side buds of the explants can be observed to germinate;
step 104: cutting off sterile 0.5-1 cm sprouts from explants, inoculating the cut sprouts into a multiplication culture medium for multiplication culture, wherein the multiplication culture medium is MS +1.0 mg/L6-BA +0.5mg/L IBA, and after 1 month, the bases of the sterile sprouts sprout to sprout new buds to gradually form cluster buds; cutting cluster buds, continuously culturing the same formula, and subculturing and proliferating once a month to ensure stable proliferation of aseptic seedlings;
step 105: cutting the cluster seedlings to obtain the single sterile seedlings for later use.
Preferably, in the step 2, the preparation of the color medium comprises the following specific steps: edible pigments with different concentrations and different colors are added into a basic culture medium required by the landscaping plant, the basic culture medium is 1/4MS + 3% sucrose +8g/L agar, and the range of the different concentrations is 40-600 mg/L.
Preferably, in the step 3, the sterile test tube has a diameter of 3-4cm and a height of 7-8 cm.
Preferably, the sterilized colored glass diamond is added into the micro-culture medium in a first mode and/or a second mode:
the first method is as follows: after the sterile test tube is subpackaged with the color culture medium in the step 3, immediately putting the glass diamond into the culture medium which is not solidified by using sterile forceps;
the second method comprises the following steps: after the medium had semi-solidified or completely solidified, the glass diamond was placed with sterile forceps.
Preferably, the plan view and the perspective view in step 4 are printed in a 1:1 ratio.
Preferably, the diameter of the plan view is 3-4cm, and the height of the elevation view is 4-5 cm.
The invention utilizes the plant in vitro culture technology to perform aseptic and plant proliferation induction on suitable varieties in a laboratory, and obtains a large amount of miniature aseptic seedlings as landscaping materials. By utilizing the characteristics of miniature individual and beautiful posture, in a test tube (a container with the diameter of 3-4cm and the height of 7-8cm and capable of being sealed) containing a colored miniature culture medium, other in-vitro culture intermediate materials (such as calluses, cluster buds, aseptic seedlings and the like which can be viewed by other species) are independently used or matched with the test tube, and the development of the test tube micro-landscape artwork is completed by combining the natural beauty of plants by using an art method.
The process flow combines the micro landscape object manufacture with the drawing, has a unified plan view to control the position of the material, and a vertical view to control the height of the material, solves the problem of poor consistency of the artistic product in production, and can produce the artwork with a certain theme and consistent effect by different producers in batch production.
The above, as well as additional purposes, advantages, and features of the present invention will become apparent to those of ordinary skill in the art upon examination of the following detailed description of specific embodiments of the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the following examples, the process flow of the test tube rose microlandscape artwork is specifically described by taking the miniature rose variety 'purple age' as an example. In other embodiments, other plant species that can be miniaturized may also be employed.
Step 1: obtaining the landscaping plant aseptic seedling which is cultured for multiple generations and cultured stably with added value. The specific steps of step 1 are shown in step 101 to step 105.
Step 101: taking annual branches of healthy, disease and insect pest-free adult landscaping plants with typical variety characteristics planted in fields.
Step 102: and (3) explant disinfection treatment: removing skin thorn and leaf on the branch, shearing 1-1.5 cm stem with bud, washing with running water for 2h, submerging explant with 75% alcohol in a super clean bench, shaking for 40s, and using 0.1% HgCl2Soaking in the solution for 5min, washing with sterile water for 3 times, and drying with sterile filter paper.
Step 103: obtaining and proliferating aseptic seedlings: inoculating the treated stem segments of the landscaping plants to a sterile bud induction culture medium which is MS + 3% sucrose +8g/L agar powder, inoculating one explant to each bottle, and observing the side bud germination of the explants after 10 days.
Step 104: cutting sterile 0.5-1 cm sprouts from explants, inoculating the cut sterile sprouts into a multiplication culture medium for multiplication culture, wherein the multiplication culture medium is MS +1.0 mg/L6-BA +0.5mg/L IBA, and after 1 month, the base parts of the sterile sprouts sprout to sprout new buds to gradually form cluster buds; cutting cluster buds, continuously culturing the same formula, and subculturing and proliferating once a month to ensure stable proliferation of the aseptic seedlings.
Step 105: cutting the cluster seedlings to obtain the single sterile seedlings for later use.
Step 2: a color medium was prepared.
The preparation method of the color culture medium comprises the following specific steps: edible pigments with different concentrations and different colors are added into a basic culture medium required by the landscaping plant, wherein the basic culture medium is 1/4MS, 3% sucrose and 8g/L agar, and the range of the different concentrations is 40-600 mg/L. The pigment color types are as follows: sapphire blue, greenish blue, capsanthin, green lemon, red velvet, dark purple and the like.
And step 3: and (3) sterilizing the prepared color culture medium by high-pressure steam, pouring the sterilized and cooled color culture medium into sterile test tubes, subpackaging each sterile test tube with the culture medium occupying the height of the container 1/4-1/3, blowing off excessive water vapor on a super clean bench, and covering a cover when the culture medium is completely cooled and solidified to obtain the micro culture medium.
Placing the prepared culture medium with various colors in a high-pressure steam sterilization pot, and sterilizing at 121 deg.C for 30 min. In the superclean bench, the sterilized culture medium is placed in a proper cooling mode, when the temperature is about 60 ℃, the culture medium is poured into sterile test tubes with the diameter of 3-4cm and the height of about 7-8cm, each test tube is filled with the culture medium occupying the height of 1/4-1/3, air is blown on the superclean bench for 30min, redundant water vapor is blown off, and after the culture medium is completely cooled and solidified, a cover is covered for later use.
In order to increase the enjoyment, interest and connotation of the culture medium, 1-9 colored glass diamonds with the diameter of 6-18 mm can be added into the colored micro culture medium. Firstly, various types of colored glass diamonds are cleaned and aired, wrapped by kraft paper, put into a sterilization pot, and sterilized at the high temperature of 121 ℃ for 30min for later use. Then, the sterilized colored glass diamond is added into the micro-culture medium in a mode I, a mode II or a combination of the mode I and the mode II.
The first method is as follows: and (3) after the sterile test tube is subpackaged with the color culture medium in the step 3, immediately putting the glass diamond into the culture medium which is not solidified by using sterile forceps, and creating the atmosphere of the diamond deeply hidden in the seabed. The second method comprises the following steps: after the medium has been semi-set or fully set, the glass diamond is placed with sterile forceps and the diamond can appear anywhere in the space containing the medium.
And 4, step 4: carrying out pattern design of a plan view and an elevation view, wherein the diameter of the plan view is the diameter of the micro culture medium, and the plan view contains position information, type information and quantity information of plants; the height of the elevation is 1/2-4/5 of the height of the micro culture medium, and the elevation contains the height information and plant type requirements of the plant. The plan view and the perspective view in step 4 are printed in a 1:1 ratio. Wherein, when the diameter of the sterile test tube is 3-4cm and the height is about 7-8cm, the diameter of a plan view is 3-4cm, and the height of an elevation view is 4-5 cm.
And 5: and (5) manufacturing the micro landscape in the test tube. The specific manufacturing steps are as follows.
Step 501: sterile Chinese rose seedlings and clustered buds or seedlings of dendrobium officinale are used as main landscaping materials of the test tube micro landscape in the purple era, and sterile Chinese rose seedlings with proper height and quantity and matched plant materials are selected from the sterile Chinese rose seedlings in the step 1 in advance according to the requirements of drawings for later use.
Step 502: the plan view is placed under the micro-culture medium. Because the bottom of the test tube is transparent, the logo of the plan view can be seen through the bottom of the test tube.
Step 503: according to the schematic of the plan view, inoculating the aseptic seedlings with set quantity and height and the matched plant materials at the set position by using aseptic tweezers to plant the plants;
step 504: according to the display effect of the elevation map, the height of the plant is adjusted to complete the plant landscaping;
step 505: and sealing the test tube to ensure that the product is sterile so as to form the test tube micro landscape artwork.
In summary, through the steps 1 to 5, the process flow of the invention combines the micro landscape entity production with the drawing, has a uniform plan view for controlling the material appearance position and a uniform elevation view for controlling the material height, solves the problem of poor consistency of the artistic product in production, and different producers can produce the artistic product with a certain theme with consistent effect in batch production. Under the condition of normal room temperature, normal illumination is realized, watering and fertilizing are not needed, and the optimal viewing period of the test tube micro landscape artwork reaches 6-12 months.
Thus, it should be appreciated by those skilled in the art that while a number of exemplary embodiments of the invention have been illustrated and described in detail herein, many other variations or modifications consistent with the principles of the invention may be directly determined or derived from the disclosure of the present invention without departing from the spirit and scope of the invention. Accordingly, the scope of the invention should be understood and interpreted to cover all such other variations or modifications.
Claims (7)
1. A process flow of micro landscape artwork in a test tube comprises the following steps:
step 1: obtaining landscape plant aseptic seedlings which are cultured for multiple generations and stably proliferated in an increment way;
step 2: preparing a color culture medium;
and step 3: sterilizing the prepared color culture medium by high-pressure steam, pouring the sterilized and cooled color culture medium into sterile test tubes, subpackaging each sterile test tube with the culture medium occupying the height of 1/4-1/3, blowing off excessive water vapor on a super clean bench, and covering a cover when the culture medium is completely cooled and solidified to obtain a micro culture medium;
and 4, step 4: carrying out pattern design of a plan view and an elevation view, wherein the diameter of the plan view is the diameter of the micro culture medium, and the plan view contains position information, type information and quantity information of plants; the height of the elevation is 1/2-4/5 of the height of the micro culture medium, and the elevation contains height information and plant type requirements of plants;
and 5: manufacturing an in-vitro microscape, and placing the plane picture below the micro culture medium; according to the schematic of the plan, inoculating the sterile seedlings and the matched plant materials with set quantity and height at the set position by using sterile tweezers for plant cultivation; according to the display effect of the elevation map, adjusting the height of the plant to finish the plant landscaping; and sealing the test tube to ensure that the product is sterile so as to form the test tube micro landscape artwork.
2. The process flow of in vitro micro landscape artwork according to claim 1, wherein the step 1 specifically comprises the following steps:
step 101: taking annual branches of healthy, disease and insect pest-free adult landscaping plants with typical variety characteristics planted in fields;
step 102: and (3) explant disinfection treatment: removing skin thorn and leaf on the branch, shearing 1-1.5 cm stem with bud, washing with running water for 2h, submerging explant with 75% alcohol in a super clean bench, shaking for 40s, and using 0.1% HgCl2Soaking in the solution for 5min, washing with sterile water for 3 times, and drying with sterile filter paper;
step 103: obtaining and proliferating aseptic seedlings: inoculating the treated stem segments of the landscaping plants to a sterile bud induction culture medium, wherein the induction culture medium is MS + 3% sucrose +8g/L agar powder, one explant is inoculated in each bottle, and after 10 days, the side buds of the explants can be observed to germinate;
step 104: cutting off sterile 0.5-1 cm sprouts from explants, inoculating the cut sprouts into a multiplication culture medium for multiplication culture, wherein the multiplication culture medium is MS +1.0 mg/L6-BA +0.5mg/L IBA, and after 1 month, the bases of the sterile sprouts sprout to sprout new buds to gradually form cluster buds; cutting cluster buds, continuously culturing the same formula, and subculturing and proliferating once a month to ensure stable proliferation of aseptic seedlings;
step 105: cutting the cluster seedlings to obtain the single sterile seedlings for later use.
3. The process flow of in vitro microlandscape artworks according to claim 1, wherein the step 2, the color culture medium preparation comprises the following specific steps: edible pigments with different concentrations and different colors are added into a basic culture medium required by the landscaping plant, the basic culture medium is 1/4MS + 3% sucrose +8g/L agar, and the range of the different concentrations is 40-600 mg/L.
4. The process flow of the micro landscape artwork in the test tube of claim 1, wherein in the step 3, the size of the sterile test tube is 3-4cm in diameter and 7-8cm in height.
5. The process flow of in vitro micro landscape artwork according to claim 1 or 4, wherein the sterilized colored glass diamond is added to the micro culture medium in a first way and/or a second way:
the first method is as follows: after the sterile test tube is subpackaged with the color culture medium in the step 3, immediately putting the glass diamond into the culture medium which is not solidified by using sterile forceps;
the second method comprises the following steps: after the medium had semi-solidified or completely solidified, the glass diamond was placed with sterile forceps.
6. The process flow of in vitro micro landscape artwork according to claim 1, wherein the plan view and the perspective view in step 4 are printed in a ratio of 1: 1.
7. The process flow of the in-vitro micro landscape artwork according to claim 1 or 4, wherein the diameter of the plan view is 3-4cm, and the height of the elevation view is 4-5 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911219602.5A CN111642380A (en) | 2019-12-03 | 2019-12-03 | Process flow of micro landscape artwork in test tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911219602.5A CN111642380A (en) | 2019-12-03 | 2019-12-03 | Process flow of micro landscape artwork in test tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111642380A true CN111642380A (en) | 2020-09-11 |
Family
ID=72340557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911219602.5A Pending CN111642380A (en) | 2019-12-03 | 2019-12-03 | Process flow of micro landscape artwork in test tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111642380A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1374237A (en) * | 2001-03-14 | 2002-10-16 | 杭州泛邦植物细胞工程有限公司 | Closed composite plant enjoying container |
| CN101278646A (en) * | 2008-05-26 | 2008-10-08 | 福建农林大学 | Method for producing sterilized miniascape |
| CN103858760A (en) * | 2014-02-26 | 2014-06-18 | 福建省农业科学院作物研究所 | Manufacturing method of test-tube type anoectohilus formosanus |
| CN104094854A (en) * | 2014-07-30 | 2014-10-15 | 陈小超 | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof |
| CN106489739A (en) * | 2016-11-25 | 2017-03-15 | 华南农业大学 | A kind of production method of Flos Carthami Rosa floribunda test tube flower |
| CN107410022A (en) * | 2017-05-16 | 2017-12-01 | 安徽梅兰园林景观工程有限公司 | A kind of cultural method of rose tube-flower |
| CN108308026A (en) * | 2018-03-09 | 2018-07-24 | 无锡南村花卉苗木专业合作社 | A kind of Growth anddevelopment tissue culture propagation |
| CN110178729A (en) * | 2019-05-20 | 2019-08-30 | 南阳师范学院 | A kind of process flow of in vitro plant text wound product |
| CN110338058A (en) * | 2019-06-24 | 2019-10-18 | 南阳师范学院 | A kind of Growth anddevelopment test tube flowering clone and screening and in-vitro conservation method |
-
2019
- 2019-12-03 CN CN201911219602.5A patent/CN111642380A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1374237A (en) * | 2001-03-14 | 2002-10-16 | 杭州泛邦植物细胞工程有限公司 | Closed composite plant enjoying container |
| CN101278646A (en) * | 2008-05-26 | 2008-10-08 | 福建农林大学 | Method for producing sterilized miniascape |
| CN103858760A (en) * | 2014-02-26 | 2014-06-18 | 福建省农业科学院作物研究所 | Manufacturing method of test-tube type anoectohilus formosanus |
| CN104094854A (en) * | 2014-07-30 | 2014-10-15 | 陈小超 | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof |
| CN106489739A (en) * | 2016-11-25 | 2017-03-15 | 华南农业大学 | A kind of production method of Flos Carthami Rosa floribunda test tube flower |
| CN107410022A (en) * | 2017-05-16 | 2017-12-01 | 安徽梅兰园林景观工程有限公司 | A kind of cultural method of rose tube-flower |
| CN108308026A (en) * | 2018-03-09 | 2018-07-24 | 无锡南村花卉苗木专业合作社 | A kind of Growth anddevelopment tissue culture propagation |
| CN110178729A (en) * | 2019-05-20 | 2019-08-30 | 南阳师范学院 | A kind of process flow of in vitro plant text wound product |
| CN110338058A (en) * | 2019-06-24 | 2019-10-18 | 南阳师范学院 | A kind of Growth anddevelopment test tube flowering clone and screening and in-vitro conservation method |
Non-Patent Citations (5)
| Title |
|---|
| 刘慧: "微型月季茎段组培快繁技术研究 ", 《北方园艺》 * |
| 常钰等: "《农业科普佳作选》", 31 May 1988, 农业出版社 * |
| 康冬茹等: "新型试管花卉姬松高效再生体系的研究 ", 《园艺学报》 * |
| 沈春修: "微型月季的组织培养技术研究 ", 《安徽农学通报(上半月刊)》 * |
| 耿晓东: "微型月季"金太阳"组织培养技术研究 ", 《江苏农业科学》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110547198B (en) | Method for rapidly propagating tillandsia through seed tissue culture | |
| CN102090337A (en) | Rapid propagation method of rhododendron latoucheae | |
| CN109287486B (en) | Paphiopedilum seed germination rate improving method and paphiopedilum cultivation method | |
| CN104585037A (en) | Tissue culture rapid-propagation method of beaucarnea recurvata | |
| CN115474546B (en) | Breeding method of columbin flowers | |
| CN114902961A (en) | Method for creating new ornamental pineapple variety by adopting EMS mutagenesis | |
| CN105028194A (en) | Tissue culture rapid propagation method of tillandsia | |
| CN104642141B (en) | Gerbera adventitious bud induction and plant regeneration method by using root as explant | |
| CN109287487B (en) | Seed germination rate improving method and cultivation method for paphiopedilum makino | |
| CN108739396B (en) | Rapid seedling method for stem segments of red honeysuckle | |
| CN106106178A (en) | A kind of method for tissue culture of confection Rhizoma Iridis Tectori | |
| CN102342246A (en) | Rhododendron decorum tissue-culture quick propagation method | |
| CN110663549B (en) | Sterile sowing and seedling raising method for dendrobium hybrid seeds | |
| CN110800609B (en) | Method for artificially and rapidly propagating rhynchophylla by utilizing embryogenic callus | |
| CN103416302A (en) | Method for culturing regeneration plant of somatic embryo of osmanthus fragrans Lour | |
| CN111642380A (en) | Process flow of micro landscape artwork in test tube | |
| CN106561453B (en) | The rapid propagation method of Campanumoea javanica | |
| CN109566417A (en) | A kind of method for tissue culture of cordyceps sinensis ginseng | |
| KR100717898B1 (en) | Methods of seed germination and bud dormancy-breaking of lady's slipper orchid, cypripedium macranthum sw | |
| CN105875410A (en) | Rapid propagation method for hybrid cymbidium seedlings of cymbidium sinense and cymbidium hookerianum | |
| CN115968783A (en) | Rapid propagation method of euphorbia pekinensis | |
| CN109717080A (en) | A method of improving Afriocan agapanthus cells,primordial subculture effect | |
| Chiu et al. | In vitro flowering and breeding of Erycina pusilla | |
| CN100409740C (en) | Medium for culturing grown-up cockscombin sealed vessel, and culture method for induction blossom | |
| CN104429964B (en) | A kind of purple chinese cymbidium tissue culture and rapid propagation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200911 |
|
| RJ01 | Rejection of invention patent application after publication |