CN110338058A - A kind of Growth anddevelopment test tube flowering clone and screening and in-vitro conservation method - Google Patents
A kind of Growth anddevelopment test tube flowering clone and screening and in-vitro conservation method Download PDFInfo
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- CN110338058A CN110338058A CN201910549232.5A CN201910549232A CN110338058A CN 110338058 A CN110338058 A CN 110338058A CN 201910549232 A CN201910549232 A CN 201910549232A CN 110338058 A CN110338058 A CN 110338058A
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- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 title claims abstract description 63
- 238000012360 testing method Methods 0.000 title claims abstract description 57
- 238000000338 in vitro Methods 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 41
- 238000012216 screening Methods 0.000 title claims abstract description 33
- 230000008774 maternal effect Effects 0.000 claims abstract description 30
- 239000000463 material Substances 0.000 claims abstract description 17
- 230000035755 proliferation Effects 0.000 claims abstract description 14
- 238000013377 clone selection method Methods 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 18
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- 239000000654 additive Substances 0.000 claims description 8
- 230000000996 additive effect Effects 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 6
- 241000345476 Agistemus Species 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 230000003321 amplification Effects 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 229960002523 mercuric chloride Drugs 0.000 claims description 3
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 231100000241 scar Toxicity 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000010008 shearing Methods 0.000 claims description 2
- 239000002304 perfume Substances 0.000 claims 1
- 238000012136 culture method Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 35
- 238000005520 cutting process Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 235000011449 Rosa Nutrition 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 240000008254 Rosa chinensis Species 0.000 description 1
- 235000000664 Rosa chinensis Nutrition 0.000 description 1
- 241001400675 Sympodium Species 0.000 description 1
- 244000223014 Syzygium aromaticum Species 0.000 description 1
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of Growth anddevelopment test tube flowering clone and screening and in-vitro conservation method, passes through and chooses donor maternal plant, choose maternal plant explant, obtain sterile maternal plant, carry out sterile seedling proliferation and test tube flowering Clone Selection；Obtain explant, Plantlet in vitro；And obtained test tube flowering clone.Materials are convenient, easily acquisition sterilizable material；The clonal screening of test tube flowering, proliferation and in-vitro culture method are simple and easy, can be the mass production of the subsequent in vitro plant microlandschaft art work, provide abundance and make scape plant；This patent especially screens the clonal material for capableing of test tube flowering, further increases the ornamental value and amusement interest of in vitro plant microlandschaft.
Description
Technical field
The present invention relates to fancy horticulture technical fields, make floristic enrich of scape in vitro plant microlandschaft and provide more
Selection more particularly to a kind of clonal screening of Growth anddevelopment test tube flowering and in-vitro conservation method.
Background technique
In vitro plant microlandschaft (Terrarium in vitro), being will be with ornamental value by Techniques of in Vitro Culture
It after plant micro, carries out making scape using artistic technique combination plant natural beauty in sterile chamber, and is equipped with and varies in color
Culture medium and accessories, what is finally constituted has the miniature artistic view of unique ornamental value.Such as to carry out in vitro plant microlandschaft
The exploitation of product, suitable ornamental plant screening and Plantlet in vitro, in case use when making scape in vitro, is primary research and development task.
' Herba Polygalae megalophae ' belongs to Rosa Growth anddevelopment cultivar, is perennial dwarf form shrub, how upright stem is, plant shape
Shorter and smaller compact, plant height is usually no more than 30cm；Plant stalk diameter is mostly in 4mm hereinafter, internode is shorter, and branches and leaves are more
It is very thin；Flower fasciation is more compact, is often arranged in sympodium, flower amount is larger, and pattern is light pink, 2~4cm of Hua Jingyue；Because of the Chinese rose
Short and small compact, the blade branch of kind plant shape and the petite lovely feature of flower, having, which becomes in vitro plant microlandschaft, makes scape plant
Inherent advantage, therefore how by the modern rose cultivars carry out in vitro culture, screening be capable of test tube flowering clone and be subject to from
Body saves, and is a technical problem to be solved urgently to one skilled in the art.
Summary of the invention
The object of the present invention is to provide a kind of Growth anddevelopment test tube flowering clone and screening and in-vitro conservation methods.This hair
It is bright to adopt the technical scheme that:
A kind of clonal screening technique of Growth anddevelopment test tube flowering chooses donor maternal plant；
It chooses maternal plant explant: choosing annual, diameter 2mm on the donor maternal plant, have the healthy and strong branch of axillary bud
As explant, the explant is handled, stem section shearing of the length about 1.5-2cm with leaf scar；
Carry out disinfection to maternal plant explant: the sterilization method is first sufficiently to flood explant with 75% alcohol, sterilizes 30s
Left and right；It uses aseptic water washing 3 times later, then with 0.1% mercuric chloride immersion treatment 8min；It is later same to use aseptic water washing 3 times,
Explant is placed on sterilized filter paper to blot partial moisture spare later；
It obtains sterile maternal plant: explant is inoculated on sterile maternal plant screening and culturing medium, an explant is inoculated in every bottle
Illumination cultivation is carried out, sterile maternal plant is obtained；
It carrying out sterile seedling proliferation and test tube flowering Clone Selection: sterile maternal plant is cut, each knife in left and right chamfers stem section,
The aseptic seedling for organizing the formation of wedge base by the sterile maternal plant of 1-2mm is formed, aseptic seedling is transferred to flowering asexual system sieve in time
Choosing and proliferated culture medium, for the proliferation of aseptic seedling and the screening of test tube flowering material, illumination cultivation will have after inoculation 7-8 weeks
There is the sterilizable material single plant of test tube flowering to be determined as test tube flowering clone.
Further, a kind of clonal screening technique of Growth anddevelopment test tube flowering, it is raw that the Growth anddevelopment chooses 2-3
Growth anddevelopment is donor maternal plant.
Further, a kind of clonal screening technique of Growth anddevelopment test tube flowering, the culture medium be MS+3% sucrose+
0.8% agar, wherein MS molysite additive amount is 1.25X, pH6.0.
Further, a kind of clonal screening technique of Growth anddevelopment test tube flowering, the flowering asexual system screening and increasing
Growing culture medium is+0.8% agar of MS+BA 0.5mg/L+IBA 0.1mg/L+3% sucrose, and wherein MS molysite additive amount is
1.25X pH6.0.
The present invention also provides a kind of clonal in-vitro conservation methods of Growth anddevelopment test tube flowering, obtain aseptic seedling explant
Body: taking test tube flowering clone aseptic seedling, and branch is divided into 2 parts, including there are the clump of about 3-5mm sprig branches base portion and to cut
At being about 5~7mm Agistemus exsertus；Plantlet in vitro: aseptic seedling explant is subjected to Plantlet in vitro, the clump branch base vertical access
Culture medium, the Agistemus exsertus horizontal access Plantlet in vitro culture medium, illumination cultivation.
Further, a kind of clonal in-vitro conservation method of Growth anddevelopment test tube flowering, Plantlet in vitro culture medium are MS+
+ 0.8% agar of BA 0.5mg/L+IBA 0.1mg/L+3% sucrose, wherein MS molysite additive amount is 1.25X, pH6.0.
Further, a kind of clonal in-vitro conservation method of Growth anddevelopment test tube flowering, the in-vitro conservation method
Can be used for sterile seedling proliferation, the aseptic seedling enrichment procedure and in-vitro conservation method difference are subculture frequency, if after
The continuous amplification for obtaining aseptic seedling, according to the method for above-mentioned preparation explant, subculture is primary within each month；If Plantlet in vitro is above-mentioned
Clone only needs more packing culture mediums, every 3 months subcultures in culture bottle primary.
The present invention also provides a kind of Growth anddevelopment test tube flowering clone, using a kind of above-mentioned Growth anddevelopment test tube flowering without
Property system's screening technique obtained test tube flowering clone is screened to " Herba Polygalae megalophae ".
The present invention also provides a kind of Growth anddevelopment test tube flowering clone, using a kind of above-mentioned Growth anddevelopment test tube flowering without
Property system's aseptic seedling enrichment procedure is to the obtained test tube flowering clone of " Herba Polygalae megalophae " sterile seedling proliferation.
The invention has the benefit that the clonal screening of Growth anddevelopment test tube flowering of the present invention and in-vitro conservation method take
Material is convenient, easily acquisition sterilizable material；The clonal screening of test tube flowering, proliferation and in-vitro culture method are simple and easy, after being
The mass production for continuing the in vitro plant microlandschaft art work provides abundance and makes scape plant；The present invention especially screens being capable of test tube flowering
Clonal material, further increase in vitro plant microlandschaft ornamental value and amusement interest.
Specific embodiment
Further describe technical solution of the present invention by taking Growth anddevelopment " Herba Polygalae megalophae " as an example below: (1) Growth anddevelopment is ' small
The selection of cloves ' in vitro culture material donor:
It is raw Growth anddevelopment " Herba Polygalae megalophae " to choose 2-3, no disease and pests harm, individual is healthy and strong, and the plant with kind typicalness makees
For donor maternal plant.
(2) selection of in vitro culture explant:
Month current year 3-4 annual, diameter about 2mm is chosen from the donor maternal plant that step (1) determines, has the strong of axillary bud
Vigorous branches item is as explant source.
(3) processing of ' Herba Polygalae megalophae ' explant:
' Herba Polygalae megalophae ' the healthy and strong branch of acquisition diameter in 2mm from the explant source that step (2) select；It is in vitro to reduce
The pollution rate of culture can remove axillary bud, blade and extra prickle on branch, and rinse 1-1.5h in tap water down-flow water；Disinfection
It according to an explant is that stem section of the length about 1.5-2cm with leaf scar is sheared by branch using dissecting scissors before processing,
Caution area division aspect upper and lower side, morphology upper end cut flat face, and morphology lower end cuts into inclined-plane.It is placed into ultra-clean work later
Sterilizing operation is carried out on platform.
(4) sterilizing operation of ' Herba Polygalae megalophae ' explant:
Sterilization method is first to be poured into obtain in the small beaker of explant equipped with step (3) with 75% alcohol, and sufficiently flood
Explant, disinfection 30s or so；It uses aseptic water washing 3 times, then with 0.1% mercuric chloride immersion treatment 8min, during which constantly shakes later
Small beaker is swung, to guarantee that each explant disinfection is thorough；Later same to use aseptic water washing 3 times, later by explant, it is poured on
Partial moisture is blotted on sterilized filter paper, it is spare.
(5) acquisition of ' Herba Polygalae megalophae ' sterile maternal plant:
By explant stem section after step (4) disinfection, sterile maternal plant screening and culturing medium (MS is inoculated into according to morphology upper and lower side
+ 0.8% agar of+3% sucrose, wherein MS molysite additive amount is 1.25X, pH6.0) on, an explant, illumination are inoculated in every bottle
Culture；After inoculation 10-12 days, observation explant is grown, and after axillary bud sprouting and pollution condition, discards pollution and without sprouting material
Material, remaining materials for later use obtain ' Herba Polygalae megalophae ' sterile maternal plant at this time.
(6) ' Herba Polygalae megalophae ' sterile seedling proliferation and test tube flowering Clone Selection:
After obtaining ' Herba Polygalae megalophae ' sterile maternal plant from step (5), the cutting method of sterile bud is as follows, and the stem section of sprouting is placed in
On aseptic filter paper, the fixed stem section of tweezers, with scalpel, each knife in left and right chamfers stem section, forms one and organizes shape by 1-2mm maternal plant
Above-mentioned seedling is transferred to the screening of flowering asexual system and proliferated culture medium (MS+BA 0.5mg/L by the aseptic seedling of wedgewise base portion in time
+ 0.8% agar of+IBA 0.1mg/L+3% sucrose, wherein MS molysite additive amount is 1.25X, pH6.0), the increasing for aseptic seedling
Grow the screening with test tube flowering material, illumination cultivation；After inoculation 7-8 weeks, sterile seedling proliferation and test tube flowering situation are observed, will be had
There is the sterilizable material single plant of test tube flowering to be determined as test tube flowering clone, and name XDX-KL1,2..... for protecting in vitro
It deposits, the material that test tube flowering is not observed is abandoned.
(7) the clonal Plantlet in vitro of ' Herba Polygalae megalophae ' test tube flowering:
By step (6) obtain test tube flowering Miao Cong handle in following manner, so as to expand rapidly on Plantlet in vitro
State test tube flowering clone:
Test tube flowering clone aseptic seedling is taken, is first picked the tissue of the extra callus of seedling base portion and blackening with scalpel
Remove, after aseptic seedling branch of growing thickly is divided from base portion by one clump, 2-3 branch, then cut in the lower part of every clump of branch, by branch
Item is divided into 2 parts, a part be there are the clump of about 3-5mm sprig branch base portion, it is spare；Another part is scattered branch, is cut
Except terminal bud on branch and extra blade；Remaining branch is formed into length by section dissection (having 2-3 section on i.e. each cutting shoots)
About 5~7mm Agistemus exsertus, it is spare.
Explant obtained by the above method is used equally for the clonal Plantlet in vitro of ' Herba Polygalae megalophae ' test tube flowering, wherein Cong Zhiji
Portion accesses culture medium vertically, and Agistemus exsertus horizontal accesses culture medium, can act as preferable aseptic seedling cultivation effect, culture medium
It is all the screening of flowering asexual system and the proliferated culture medium of step (6)；Proliferation and Plantlet in vitro difference are subculture frequency, if after
The continuous amplification for obtaining aseptic seedling, according to the method for above-mentioned preparation explant, subculture is primary within each month；If Plantlet in vitro is above-mentioned
Clone only needs more packing culture mediums, every 3 months subcultures in culture bottle primary；Above-mentioned culture is illumination cultivation.
Claims (9)
1. a kind of clonal screening technique of Growth anddevelopment test tube flowering, it is characterised in that:
Choose donor maternal plant；
It chooses maternal plant explant: choosing annual, diameter 2mm on the donor maternal plant, have the healthy and strong branch conduct of axillary bud
Explant handles the explant, stem section shearing of the length about 1.5-2cm with leaf scar；
Carry out disinfection to maternal plant explant: the sterilization method is first sufficiently to flood explant with 75% alcohol, and disinfection 30s is left
It is right；It uses aseptic water washing 3 times later, then with 0.1% mercuric chloride immersion treatment 8min；Later same to use aseptic water washing 3 times, it
Explant is placed on sterilized filter paper to blot partial moisture spare afterwards；
It obtains sterile maternal plant: explant is inoculated on sterile maternal plant screening and culturing medium, an explant is inoculated in every bottle and is carried out
Illumination cultivation obtains sterile maternal plant；
It carries out sterile seedling proliferation and test tube flowering Clone Selection: sterile maternal plant is cut, each knife in left and right chamfers stem section, is formed
One is organized the formation of the aseptic seedling of wedge base by the sterile maternal plant of 1-2mm, aseptic seedling is transferred in time flowering asexual system screening and
Proliferated culture medium, for the proliferation of aseptic seedling and the screening of test tube flowering material, illumination cultivation will have examination after inoculation 7-8 weeks
The sterilizable material single plant that pipe is bloomed is determined as test tube flowering clone.
2. a kind of clonal screening technique of Growth anddevelopment test tube flowering according to claim 1, it is characterised in that: described
It is donor maternal plant that Growth anddevelopment, which chooses the raw Growth anddevelopment of 2-3,.
3. a kind of clonal screening technique of Growth anddevelopment test tube flowering according to claim 1, it is characterised in that: described
Culture medium is+0.8% agar of MS+3% sucrose, and wherein MS molysite additive amount is 1.25X, pH6.0.
4. a kind of clonal screening technique of Growth anddevelopment test tube flowering according to claim 1, it is characterised in that: described
The screening of flowering asexual system and proliferated culture medium are+0.8% agar of MS+BA 0.5mg/L+IBA 0.1mg/L+3% sucrose, wherein
MS molysite additive amount is 1.25X, pH6.0.
5. a kind of clonal in-vitro conservation method of Growth anddevelopment test tube flowering, it is characterised in that:
It obtains aseptic seedling explant: taking test tube flowering clone aseptic seedling, branch is divided into 2 parts, including there are about 3-5mm
It the clump branch base portion of sprig and is cut into and is about 5~7mm Agistemus exsertus；
Plantlet in vitro: aseptic seedling explant is subjected to Plantlet in vitro, the clump branch base vertical accesses culture medium, and the tool saves stem
Section horizontal accesses Plantlet in vitro culture medium, illumination cultivation.
6. a kind of clonal in-vitro conservation method of Growth anddevelopment test tube flowering according to claim 5, it is characterised in that:
Plantlet in vitro culture medium is+0.8% agar of MS+BA 0.5mg/L+IBA 0.1mg/L+3% sucrose, wherein MS molysite additive amount
For 1.25X, pH6.0.
7. a kind of clonal in-vitro conservation method of Growth anddevelopment test tube flowering according to claim 5, it is characterised in that:
The in-vitro conservation method can also be used for sterile seedling proliferation, and the aseptic seedling enrichment procedure and the in-vitro conservation method are distinguished
In subculture frequency, if continuing the amplification of acquisition aseptic seedling, according to the method for above-mentioned preparation explant, each month subculture one
It is secondary；If the above-mentioned clone of Plantlet in vitro, only need more packing culture mediums, every 3 months subcultures in culture bottle primary.
8. a kind of Growth anddevelopment test tube flowering clone, it is characterised in that: using claim 1 screening technique to " small fourth
It is fragrant " the obtained test tube flowering clone of screening.
9. a kind of Growth anddevelopment test tube flowering clone, it is characterised in that: using claim 7 screening technique to " small fourth
The sterile obtained test tube flowering clone of seedling proliferation of perfume ".
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110839530A (en) * | 2019-11-25 | 2020-02-28 | 航天神舟生物科技集团有限公司 | Method for inducing flowering of Chinese rose in test tube |
| CN111642380A (en) * | 2019-12-03 | 2020-09-11 | 南阳师范学院 | Process flow of micro landscape artwork in test tube |
-
2019
- 2019-06-24 CN CN201910549232.5A patent/CN110338058A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| 孙朝辉等: "‘香槟’月季的组织培养和试管开花诱导", 《植物生理学报》 * |
| 朱逢玲: "几种微景观植物的快繁及微型化处理研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 焦晓琳: "离体植物微景观微型月季产品的研发", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 赵丽达: "适合离体微景观月季品种的筛选及应用", 《南阳师范学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110839530A (en) * | 2019-11-25 | 2020-02-28 | 航天神舟生物科技集团有限公司 | Method for inducing flowering of Chinese rose in test tube |
| CN111642380A (en) * | 2019-12-03 | 2020-09-11 | 南阳师范学院 | Process flow of micro landscape artwork in test tube |
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Application publication date: 20191018 |